MD12G1085300.v1.1
NAC Family

inactive purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0007682
Physical Location & Seq
Reverse (-)
10431860 .. 10436153
4294 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1085300.v1.1.491

Sequence Viewer

Length: 927 bp
ATGTTCTTTTTTATGTTTCACATTTCTGGTTGTTGTCTTGAAGCTGATTTCGTTATCTATCTCGGAGATGTCATCACAGCCAACAATATAGCGATTAAAAATGCAAGCTTGTACTGGGATCAGGCAGTTTCTCCAACAAGAGCTAGGGGTATTCCTTGGGCAAGTGTGTTTGGAAACCACGATGATGCAGCATTTGTGTGGCCAATTGAGTGGTTTTCATCCCCTGGAATTCCTGAAATTCGTTGCCGTGTAGCCAATTCATCATGCTCAGAAGAAGAGTGTCGCTTTGAGGGCACACAACGTCTGGGGTTAATGAAAAATGAGATTGAGCGTAACACGCTATCATACTCTCGATATGGTCCTAAAGAACTTTGGCCTAGTATATCCAACTATGTCCTCCAACTTTTGTCGTCCGACAATCCCAAGTCACCTGTGGCATTTTTGTACTTCCTAGATTCTGGTGGTGGTTCCTACCCAGAAATTATATCCAGTGCCCAAGCAGAATGGTTCCGCCAGAAAGCTCTAGAGATGAACCCTGATTCAAGGGTGCCAGAGATAATCTTTTGGCATATACCAAGTCGTGCTTATAAGAAGGCGGCTCCATGGTTCAGTATACACAAGCCTTGTGTGGGTTCAATTAACAAGGAGAGAGTAGCAACTCAAGAAGCTGAAATGGGTATCATGAAACTTTTGGTTGAAAGGCCTTCTGCCAAGGCAATATTTGTAGGACACAACCATGGATTAGACTGGTGTTGCCCCTACGAAAAACTGTGGCTATGTTTTGCTCGGCATACCGGTTATGGTGGGTACGGAAACTGGGACAGAGGAGCTAGAATTGTGGAAATCACCGAGCAGCCTTTCTCTGTTAAATCTTGGATAAGAATGGAGGATGGTAGTGTACATAGTGAAGTTGTCTTGAGTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0005575 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

309

Amino Acids

34.97

Weight (kDa)

6.09

Isoelectric Point (pI)

45.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 14 - 246 2.5e-07 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 588
AccB1I GGYRCC 1 cut(s) 547
AccI GTMKAC 1 cut(s) 613
AciI CCGC 2 cut(s) 511, 596
AclWI GGATC 1 cut(s) 126
AcoI YGGCCR 1 cut(s) 200
AcsI RAATTY 2 cut(s) 228, 237
AfaI GTAC 4 cut(s) 113, 446, 809, 900
AfiI CCNNNNNNNGG 1 cut(s) 629
AgeI ACCGGT 1 cut(s) 794
AgsI TTSAA 4 cut(s) 41, 543, 636, 698
AjnI CCWGG 1 cut(s) 223
AluBI AGCT 6 cut(s) 44, 108, 143, 521, 668, 830
AluI AGCT 6 cut(s) 44, 108, 143, 521, 668, 830
AlwI GGATC 1 cut(s) 126
AoxI GGCC 3 cut(s) 200, 374, 701
ApeKI GCWGC 2 cut(s) 188, 853
ApoI RAATTY 2 cut(s) 228, 237
AsiGI ACCGGT 1 cut(s) 794
AspS9I GGNCC 1 cut(s) 359
AsuHPI GGTGA 2 cut(s) 420, 838
AvaII GGWCC 1 cut(s) 359
BaeGI GKGCMC 2 cut(s) 296, 496
BalI TGGCCA 1 cut(s) 202
BanI GGYRCC 1 cut(s) 547
BbvI GCAGC 2 cut(s) 200, 865
BccI CCATC 1 cut(s) 884
BceAI ACGGC 1 cut(s) 231
BciT130I CCWGG 1 cut(s) 225
BfaI CTAG 5 cut(s) 144, 378, 452, 524, 831
BisI GCNGC 3 cut(s) 189, 597, 854
BlsI GCNGC 3 cut(s) 190, 598, 855
Bme1390I CCNGG 1 cut(s) 225
Bme18I GGWCC 1 cut(s) 359
BmgT120I GGNCC 1 cut(s) 359
BmiI GGNNCC 4 cut(s) 469, 509, 549, 600
BmrFI CCNGG 1 cut(s) 225
BmrI ACTGGG 2 cut(s) 124, 826
BmsI GCATC 1 cut(s) 175
BmuI ACTGGG 2 cut(s) 124, 826
BpuEI CTTGAG 1 cut(s) 645
BsaJI CCNNGG 5 cut(s) 155, 223, 602, 711, 736
BsaWI WCCGGW 1 cut(s) 794
Bsc4I CCNNNNNNNGG 1 cut(s) 629
Bse118I RCCGGY 1 cut(s) 794
Bse1I ACTGG 4 cut(s) 119, 489, 752, 821
BseBI CCWGG 1 cut(s) 225
BseDI CCNNGG 5 cut(s) 155, 223, 602, 711, 736
BseGI GGATG 2 cut(s) 218, 895
BseLI CCNNNNNNNGG 1 cut(s) 629
BseMII CTCAG 1 cut(s) 282
BseNI ACTGG 4 cut(s) 119, 489, 752, 821
BseRI GAGGAG 1 cut(s) 840
BseSI GKGCMC 2 cut(s) 296, 496
BseXI GCAGC 2 cut(s) 200, 865
BshFI GGCC 3 cut(s) 202, 376, 703
BshNI GGYRCC 1 cut(s) 547
BshTI ACCGGT 1 cut(s) 794
BsiSI CCGG 1 cut(s) 795
BslFI GGGAC 1 cut(s) 833
BslI CCNNNNNNNGG 1 cut(s) 629
BsmFI GGGAC 1 cut(s) 833
BsnI GGCC 3 cut(s) 202, 376, 703
Bsp1286I GDGCHC 2 cut(s) 296, 496
Bsp1407I TGTACA 1 cut(s) 898
Bsp143I GATC 1 cut(s) 118
Bsp19I CCATGG 2 cut(s) 602, 736
BspACI CCGC 2 cut(s) 511, 596
BspANI GGCC 3 cut(s) 202, 376, 703
BspCNI CTCAG 1 cut(s) 281
BspHI TCATGA 1 cut(s) 681
BspLI GGNNCC 4 cut(s) 469, 509, 549, 600
BspPI GGATC 1 cut(s) 126
BspT107I GGYRCC 1 cut(s) 547
BsrFI RCCGGY 1 cut(s) 794
BsrGI TGTACA 1 cut(s) 898
BsrI ACTGG 4 cut(s) 119, 489, 752, 821
BssAI RCCGGY 1 cut(s) 794
BssECI CCNNGG 5 cut(s) 155, 223, 602, 711, 736
BssMI GATC 1 cut(s) 118
BssNAI GTATAC 1 cut(s) 614
BssT1I CCWWGG 4 cut(s) 155, 602, 711, 736
Bst1107I GTATAC 1 cut(s) 614
Bst2UI CCWGG 1 cut(s) 225
Bst4CI ACNGT 1 cut(s) 771
Bst6I CTCTTC 1 cut(s) 270
BstAUI TGTACA 1 cut(s) 898
BstC8I GCNNGC 1 cut(s) 106
BstDEI CTNAG 1 cut(s) 268
BstDSI CCRYGG 2 cut(s) 602, 736
BstF5I GGATG 2 cut(s) 218, 895
BstKTI GATC 1 cut(s) 121
BstMBI GATC 1 cut(s) 118
BstMWI GCNNNNNNNGC 2 cut(s) 291, 337
BstNI CCWGG 1 cut(s) 225
BstSCI CCNGG 1 cut(s) 223
BstSLI GKGCMC 2 cut(s) 296, 496
BstV1I GCAGC 2 cut(s) 200, 865
BstXI CCANNNNNNTGG 1 cut(s) 210
BstZ17I GTATAC 1 cut(s) 614
BsuRI GGCC 3 cut(s) 202, 376, 703
BtgI CCRYGG 2 cut(s) 602, 736
BtsCI GGATG 2 cut(s) 218, 895
BtsIMutI CAGTG 1 cut(s) 496
Cac8I GCNNGC 1 cut(s) 106
CciI TCATGA 1 cut(s) 681
Cfr10I RCCGGY 1 cut(s) 794
Cfr13I GGNCC 1 cut(s) 359
Csp6I GTAC 4 cut(s) 112, 445, 808, 899
CspAI ACCGGT 1 cut(s) 794
CviAII CATG 4 cut(s) 264, 603, 682, 737
CviQI GTAC 4 cut(s) 112, 445, 808, 899
DdeI CTNAG 1 cut(s) 268
DpnI GATC 1 cut(s) 120
DpnII GATC 1 cut(s) 118
EaeI YGGCCR 1 cut(s) 200
Eam1104I CTCTTC 1 cut(s) 270
EarI CTCTTC 1 cut(s) 270
EciI GGCGGA 1 cut(s) 500
Eco130I CCWWGG 4 cut(s) 155, 602, 711, 736
Eco147I AGGCCT 1 cut(s) 703
Eco47I GGWCC 1 cut(s) 359
EcoRI GAATTC 1 cut(s) 228
EcoRII CCWGG 1 cut(s) 223
EcoT14I CCWWGG 4 cut(s) 155, 602, 711, 736
ErhI CCWWGG 4 cut(s) 155, 602, 711, 736
FaeI CATG 4 cut(s) 267, 606, 685, 740
FalI AAGNNNNNCTT 2 cut(s) 568, 600
FaqI GGGAC 1 cut(s) 833
FatI CATG 4 cut(s) 263, 602, 681, 736
FblI GTMKAC 1 cut(s) 613
Fnu4HI GCNGC 3 cut(s) 189, 597, 854
FokI GGATG 2 cut(s) 205, 902
Fsp4HI GCNGC 3 cut(s) 189, 597, 854
FspBI CTAG 5 cut(s) 144, 378, 452, 524, 831
GluI GCNGC 3 cut(s) 189, 597, 854
HaeIII GGCC 3 cut(s) 202, 376, 703
HapII CCGG 1 cut(s) 795
Hin1II CATG 4 cut(s) 267, 606, 685, 740
HindIII AAGCTT 1 cut(s) 106
HinfI GANTC 3 cut(s) 455, 539, 919
HpaII CCGG 1 cut(s) 795
HphI GGTGA 2 cut(s) 420, 838
Hpy166II GTNNAC 2 cut(s) 614, 899
Hpy188I TCNGA 3 cut(s) 65, 271, 415
Hpy188III TCNNGA 7 cut(s) 38, 233, 351, 524, 662, 682, 916
Hpy8I GTNNAC 2 cut(s) 614, 899
HpyAV CCTTC 2 cut(s) 586, 714
HpyCH4III ACNGT 1 cut(s) 771
HpyCH4IV ACGT 1 cut(s) 301
HpyCH4V TGCA 2 cut(s) 104, 188
HpyF10VI GCNNNNNNNGC 2 cut(s) 291, 337
HpyF3I CTNAG 1 cut(s) 268
HpySE526I ACGT 1 cut(s) 301
Hsp92II CATG 4 cut(s) 267, 606, 685, 740
Kzo9I GATC 1 cut(s) 118
LmnI GCTCC 2 cut(s) 604, 827
Lsp1109I GCAGC 2 cut(s) 200, 865
LweI GCATC 1 cut(s) 175
MaeI CTAG 5 cut(s) 144, 378, 452, 524, 831
MaeII ACGT 1 cut(s) 301
MaeIII GTNAC 2 cut(s) 332, 426
MalI GATC 1 cut(s) 120
MboI GATC 1 cut(s) 118
MboII GAAGA 2 cut(s) 284, 287
MfeI CAATTG 1 cut(s) 204
MhlI GDGCHC 2 cut(s) 296, 496
MlsI TGGCCA 1 cut(s) 202
MluCI AATT 7 cut(s) 204, 228, 237, 256, 480, 636, 834
MluNI TGGCCA 1 cut(s) 202
MmeI TCCRAC 4 cut(s) 158, 411, 424, 438
MnlI CCTC 4 cut(s) 283, 407, 818, 880
Mox20I TGGCCA 1 cut(s) 202
MscI TGGCCA 1 cut(s) 202
MseI TTAA 4 cut(s) 96, 311, 639, 867
MslI CAYNNNNRTG 3 cut(s) 183, 196, 735
Msp20I TGGCCA 1 cut(s) 202
MspI CCGG 1 cut(s) 795
MspR9I CCNGG 1 cut(s) 225
MunI CAATTG 1 cut(s) 204
MvaI CCWGG 1 cut(s) 225
MwoI GCNNNNNNNGC 2 cut(s) 291, 337
NcoI CCATGG 2 cut(s) 602, 736
NdeII GATC 1 cut(s) 118
NlaIII CATG 4 cut(s) 267, 606, 685, 740
NlaIV GGNNCC 4 cut(s) 469, 509, 549, 600
NmeAIII GCCGAG 1 cut(s) 766
NmuCI GTSAC 1 cut(s) 426
PagI TCATGA 1 cut(s) 681
PceI AGGCCT 1 cut(s) 703
PfeI GAWTC 2 cut(s) 455, 539
PinAI ACCGGT 1 cut(s) 794
PkrI GCNGC 3 cut(s) 190, 598, 855
PsiI TTATAA 1 cut(s) 588
Psp6I CCWGG 1 cut(s) 223
PspGI CCWGG 1 cut(s) 223
PspN4I GGNNCC 4 cut(s) 469, 509, 549, 600
PspPI GGNCC 1 cut(s) 359
RsaI GTAC 4 cut(s) 113, 446, 809, 900
RsaNI GTAC 4 cut(s) 112, 445, 808, 899
RseI CAYNNNNRTG 3 cut(s) 183, 196, 735
SaqAI TTAA 4 cut(s) 96, 311, 639, 867
SatI GCNGC 3 cut(s) 189, 597, 854
Sau3AI GATC 1 cut(s) 118
Sau96I GGNCC 1 cut(s) 359
ScrFI CCNGG 1 cut(s) 225
SduI GDGCHC 2 cut(s) 296, 496
SetI ASST 8 cut(s) 46, 110, 145, 304, 433, 523, 670, 832
SfaNI GCATC 1 cut(s) 175
SinI GGWCC 1 cut(s) 359
SmiMI CAYNNNNRTG 3 cut(s) 183, 196, 735
SmlI CTYRAG 2 cut(s) 660, 916
SmoI CTYRAG 2 cut(s) 660, 916
Sse9I AATT 7 cut(s) 204, 228, 237, 256, 480, 636, 834
SseBI AGGCCT 1 cut(s) 703
SsiI CCGC 2 cut(s) 511, 596
SspI AATATT 1 cut(s) 720
SspMI CTAG 5 cut(s) 144, 378, 452, 524, 831
StuI AGGCCT 1 cut(s) 703
StyD4I CCNGG 1 cut(s) 223
StyI CCWWGG 4 cut(s) 155, 602, 711, 736
TaaI ACNGT 1 cut(s) 771
TaiI ACGT 1 cut(s) 304
TaqI TCGA 1 cut(s) 352
TasI AATT 7 cut(s) 204, 228, 237, 256, 480, 636, 834
TatI WGTACW 3 cut(s) 111, 444, 898
TauI GCSGC 1 cut(s) 599
TfiI GAWTC 2 cut(s) 455, 539
Tru1I TTAA 4 cut(s) 96, 311, 639, 867
Tru9I TTAA 4 cut(s) 96, 311, 639, 867
TscAI CASTG 1 cut(s) 496
TseFI GTSAC 1 cut(s) 426
TseI GCWGC 2 cut(s) 188, 853
Tsp45I GTSAC 1 cut(s) 426
TspDTI ATGAA 5 cut(s) 207, 249, 329, 545, 698
TspGWI ACGGA 1 cut(s) 825
TspRI CASTG 1 cut(s) 496
VpaK11BI GGWCC 1 cut(s) 359
XapI RAATTY 2 cut(s) 228, 237
XbaI TCTAGA 1 cut(s) 523
XcmI CCANNNNNNNNNTGG 1 cut(s) 430
XmiI GTMKAC 1 cut(s) 613
XspI CTAG 5 cut(s) 144, 378, 452, 524, 831
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.