pycom12g07220
NAC Family

inactive purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0007682
Physical Location & Seq
Reverse (-)
7479863 .. 7484970
5108 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g07220.3

Sequence Viewer

Length: 1143 bp
ATGCCGTCTGTCTGCTGGCTGGTGCCCAGAGTAACTCAAGTGATGATAGCAAAACTCTTCTACTTTCTGCTCATCTCCGCCGTCGCTCTACCGACCAAGGCGGAAGAGGGTTGCCATCAGCTACGGTCCACTGCACCGTTCAAGATCGCTCTGTTTGCCGACCTGCATTTCGGAGAGGACGCATGGACCCATTGGGGTCCACTCCAGGATGTGAAGTCTATCAAGGTCATGTCCTCTGTTCTCGACGATGAAACCCCAGATTTCGTTATCTATCTTGGAGATGTCATCACAGCCAACAATATAGCGATTAAAAATGCAAGCTTGTACTGGGATCAGGCAGTTTCTCCAACAAGAGCTAGGGGTATTCCTTGGGCAAGTGTGTTTGGAAACCACGATGATGCAGCATTTGTGTGGCCAATTGAGTGGTTTTCATCCCCTGGAATTCCTGAAATTCGTTGCCACGTAGCCAATTCATCATGCTCAGGAGAAGAAGAGTGTCACTTTGAAGGCACACAACGTCTGGAGTTAATGAAAAATGAGATTGAGCGTAACACGCTATCATACTCTCGATATGGTCCTAAAGAACTTTGGCCTAGTATATCCAACTATGTCCTCCAACTTTTGTCGTCCGACAATCCCAAGTCACCTGTGGCATTATTGTACTTCCTAGATTCTGGTGGTGGTTCCTACCCAGAAATTATATCCAGTGCCCAAGCAGAATGGTTCCGCCAGAAAGCTCTAGAGATTAACCCTGATTCAAGGGTGCCGGAGATAATCTTTTGGCATATACCAAGTCGTGCTTATAAGAAGGCGGCTCCATGGTTCGGTATACACAAGCCTTGTGTGGGTTCAATTAACAAGGAGAGAGTAGCAACTCAAGAAGCTGAAATGGGTATCATGAAACTTTTGGTTGAAAGGCCTTCTGCCAAGGCAATATTTGTAGGACACAACCATGGATTAGACTGGTGTTGCCCCTACGAAAAACTGTGGCTATGTTTTGCTCGGCATACCGGTTACGGTGGGTACGGAAACTGGAACAGAGGAGCTAGAATTGTGGAAATCACCGAGCAGCCTTTCTCTGTTAAATCTTGGATAAGAATGGAGGATGGCAGTGTACATAGTGAAGTTGTCTTGAGTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0005575 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

381

Amino Acids

42.83

Weight (kDa)

5.96

Isoelectric Point (pI)

47.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 804
Acc36I ACCTGC 1 cut(s) 171
AccB1I GGYRCC 2 cut(s) 22, 763
AccI GTMKAC 1 cut(s) 829
AciI CCGC 4 cut(s) 78, 101, 727, 812
AclWI GGATC 1 cut(s) 339
AcoI YGGCCR 1 cut(s) 413
AcsI RAATTY 2 cut(s) 441, 450
AfaI GTAC 4 cut(s) 326, 662, 1025, 1116
AfiI CCNNNNNNNGG 2 cut(s) 824, 845
AgeI ACCGGT 1 cut(s) 1010
AgsI TTSAA 5 cut(s) 142, 506, 759, 852, 914
AjnI CCWGG 2 cut(s) 204, 436
AluBI AGCT 6 cut(s) 121, 321, 356, 737, 884, 1046
AluI AGCT 6 cut(s) 121, 321, 356, 737, 884, 1046
AlwI GGATC 1 cut(s) 339
AoxI GGCC 3 cut(s) 413, 590, 917
ApeKI GCWGC 2 cut(s) 401, 1069
ApoI RAATTY 2 cut(s) 441, 450
ArsI GACNNNNNNTTYG 2 cut(s) 152, 184
AsiGI ACCGGT 1 cut(s) 1010
AspS9I GGNCC 4 cut(s) 126, 186, 197, 575
AsuHPI GGTGA 2 cut(s) 636, 1054
AvaII GGWCC 4 cut(s) 126, 186, 197, 575
BaeGI GKGCMC 2 cut(s) 27, 712
BalI TGGCCA 1 cut(s) 415
BanI GGYRCC 2 cut(s) 22, 763
BbvI GCAGC 2 cut(s) 413, 1081
BccI CCATC 2 cut(s) 123, 1100
BceAI ACGGC 1 cut(s) 65
BciT130I CCWGG 2 cut(s) 206, 438
BfaI CTAG 5 cut(s) 357, 594, 668, 740, 1047
BfuAI ACCTGC 1 cut(s) 171
BisI GCNGC 3 cut(s) 402, 813, 1070
BlsI GCNGC 3 cut(s) 403, 814, 1071
Bme1390I CCNGG 2 cut(s) 206, 438
Bme18I GGWCC 4 cut(s) 126, 186, 197, 575
BmgT120I GGNCC 4 cut(s) 126, 186, 197, 575
BmiI GGNNCC 7 cut(s) 24, 188, 198, 685, 725, 765, 816
BmrFI CCNGG 2 cut(s) 206, 438
BmrI ACTGGG 1 cut(s) 337
BmsI GCATC 1 cut(s) 388
BmuI ACTGGG 1 cut(s) 337
BpmI CTGGAG 2 cut(s) 188, 542
Bpu10I CCTNAGC 1 cut(s) 481
BpuEI CTTGAG 2 cut(s) 21, 861
BsaAI YACGTR 1 cut(s) 463
BsaJI CCNNGG 6 cut(s) 96, 368, 436, 818, 927, 952
BsaWI WCCGGW 1 cut(s) 1010
Bsc4I CCNNNNNNNGG 2 cut(s) 824, 845
Bse118I RCCGGY 1 cut(s) 1010
Bse1I ACTGG 4 cut(s) 332, 705, 968, 1037
BseBI CCWGG 2 cut(s) 206, 438
BseDI CCNNGG 6 cut(s) 96, 368, 436, 818, 927, 952
BseGI GGATG 3 cut(s) 214, 431, 1111
BseLI CCNNNNNNNGG 2 cut(s) 824, 845
BseMII CTCAG 1 cut(s) 495
BseNI ACTGG 4 cut(s) 332, 705, 968, 1037
BseRI GAGGAG 1 cut(s) 1056
BseSI GKGCMC 2 cut(s) 27, 712
BseXI GCAGC 2 cut(s) 413, 1081
BsgI GTGCAG 1 cut(s) 117
BshFI GGCC 3 cut(s) 415, 592, 919
BshNI GGYRCC 2 cut(s) 22, 763
BshTI ACCGGT 1 cut(s) 1010
BsiSI CCGG 2 cut(s) 767, 1011
BslI CCNNNNNNNGG 2 cut(s) 824, 845
BsnI GGCC 3 cut(s) 415, 592, 919
Bsp1286I GDGCHC 2 cut(s) 27, 712
Bsp1407I TGTACA 1 cut(s) 1114
Bsp143I GATC 2 cut(s) 144, 331
Bsp19I CCATGG 2 cut(s) 818, 952
BspACI CCGC 4 cut(s) 78, 101, 727, 812
BspANI GGCC 3 cut(s) 415, 592, 919
BspCNI CTCAG 1 cut(s) 494
BspHI TCATGA 1 cut(s) 897
BspLI GGNNCC 7 cut(s) 24, 188, 198, 685, 725, 765, 816
BspMI ACCTGC 1 cut(s) 171
BspPI GGATC 1 cut(s) 339
BspT107I GGYRCC 2 cut(s) 22, 763
BsrFI RCCGGY 1 cut(s) 1010
BsrGI TGTACA 1 cut(s) 1114
BsrI ACTGG 4 cut(s) 332, 705, 968, 1037
BssAI RCCGGY 1 cut(s) 1010
BssECI CCNNGG 6 cut(s) 96, 368, 436, 818, 927, 952
BssMI GATC 2 cut(s) 144, 331
BssNAI GTATAC 1 cut(s) 830
BssT1I CCWWGG 5 cut(s) 96, 368, 818, 927, 952
Bst1107I GTATAC 1 cut(s) 830
Bst2UI CCWGG 2 cut(s) 206, 438
Bst4CI ACNGT 4 cut(s) 126, 138, 987, 1019
Bst6I CTCTTC 3 cut(s) 62, 99, 486
BstAUI TGTACA 1 cut(s) 1114
BstBAI YACGTR 1 cut(s) 463
BstC8I GCNNGC 2 cut(s) 17, 319
BstDEI CTNAG 1 cut(s) 481
BstDSI CCRYGG 2 cut(s) 818, 952
BstF5I GGATG 3 cut(s) 214, 431, 1111
BstKTI GATC 2 cut(s) 147, 334
BstMBI GATC 2 cut(s) 144, 331
BstMWI GCNNNNNNNGC 2 cut(s) 155, 553
BstNI CCWGG 2 cut(s) 206, 438
BstSCI CCNGG 2 cut(s) 204, 436
BstSLI GKGCMC 2 cut(s) 27, 712
BstV1I GCAGC 2 cut(s) 413, 1081
BstXI CCANNNNNNTGG 1 cut(s) 423
BstZ17I GTATAC 1 cut(s) 830
BsuRI GGCC 3 cut(s) 415, 592, 919
BtgI CCRYGG 2 cut(s) 818, 952
BtsCI GGATG 3 cut(s) 214, 431, 1111
BtsI GCAGTG 2 cut(s) 129, 1117
BtsIMutI CAGTG 3 cut(s) 129, 712, 1117
BveI ACCTGC 1 cut(s) 171
Cac8I GCNNGC 2 cut(s) 17, 319
CciI TCATGA 1 cut(s) 897
Cfr10I RCCGGY 1 cut(s) 1010
Cfr13I GGNCC 4 cut(s) 126, 186, 197, 575
CseI GACGC 1 cut(s) 188
Csp6I GTAC 4 cut(s) 325, 661, 1024, 1115
CspAI ACCGGT 1 cut(s) 1010
CviAII CATG 6 cut(s) 183, 229, 477, 819, 898, 953
CviQI GTAC 4 cut(s) 325, 661, 1024, 1115
DdeI CTNAG 1 cut(s) 481
DpnI GATC 2 cut(s) 146, 333
DpnII GATC 2 cut(s) 144, 331
EaeI YGGCCR 1 cut(s) 413
Eam1104I CTCTTC 3 cut(s) 62, 99, 486
EarI CTCTTC 3 cut(s) 62, 99, 486
EciI GGCGGA 3 cut(s) 67, 116, 716
Eco130I CCWWGG 5 cut(s) 96, 368, 818, 927, 952
Eco147I AGGCCT 1 cut(s) 919
Eco47I GGWCC 4 cut(s) 126, 186, 197, 575
EcoRI GAATTC 1 cut(s) 441
EcoRII CCWGG 2 cut(s) 204, 436
EcoT14I CCWWGG 5 cut(s) 96, 368, 818, 927, 952
ErhI CCWWGG 5 cut(s) 96, 368, 818, 927, 952
FaeI CATG 6 cut(s) 186, 232, 480, 822, 901, 956
FalI AAGNNNNNCTT 2 cut(s) 784, 816
FatI CATG 6 cut(s) 182, 228, 476, 818, 897, 952
FblI GTMKAC 1 cut(s) 829
Fnu4HI GCNGC 3 cut(s) 402, 813, 1070
FokI GGATG 3 cut(s) 221, 418, 1118
Fsp4HI GCNGC 3 cut(s) 402, 813, 1070
FspBI CTAG 5 cut(s) 357, 594, 668, 740, 1047
GluI GCNGC 3 cut(s) 402, 813, 1070
GsuI CTGGAG 2 cut(s) 188, 542
HaeIII GGCC 3 cut(s) 415, 592, 919
HapII CCGG 2 cut(s) 767, 1011
HgaI GACGC 1 cut(s) 188
Hin1II CATG 6 cut(s) 186, 232, 480, 822, 901, 956
HindIII AAGCTT 1 cut(s) 319
HinfI GANTC 3 cut(s) 671, 755, 1135
HpaII CCGG 2 cut(s) 767, 1011
HphI GGTGA 2 cut(s) 636, 1054
Hpy166II GTNNAC 4 cut(s) 129, 200, 830, 1115
Hpy188I TCNGA 2 cut(s) 173, 631
Hpy8I GTNNAC 4 cut(s) 129, 200, 830, 1115
Hpy99I CGWCG 2 cut(s) 86, 248
HpyAV CCTTC 3 cut(s) 500, 802, 930
HpyCH4III ACNGT 4 cut(s) 126, 138, 987, 1019
HpyCH4IV ACGT 2 cut(s) 462, 517
HpyCH4V TGCA 4 cut(s) 134, 166, 317, 401
HpyF10VI GCNNNNNNNGC 2 cut(s) 155, 553
HpyF3I CTNAG 1 cut(s) 481
HpySE526I ACGT 2 cut(s) 462, 517
Hsp92II CATG 6 cut(s) 186, 232, 480, 822, 901, 956
Kzo9I GATC 2 cut(s) 144, 331
LmnI GCTCC 2 cut(s) 820, 1043
Lsp1109I GCAGC 2 cut(s) 413, 1081
LweI GCATC 1 cut(s) 388
MaeI CTAG 5 cut(s) 357, 594, 668, 740, 1047
MaeII ACGT 2 cut(s) 462, 517
MaeIII GTNAC 5 cut(s) 31, 497, 548, 642, 1013
MalI GATC 2 cut(s) 146, 333
MboI GATC 2 cut(s) 144, 331
MboII GAAGA 4 cut(s) 49, 116, 500, 503
MfeI CAATTG 1 cut(s) 417
MhlI GDGCHC 2 cut(s) 27, 712
MlsI TGGCCA 1 cut(s) 415
MluCI AATT 7 cut(s) 417, 441, 450, 469, 696, 852, 1050
MluNI TGGCCA 1 cut(s) 415
MmeI TCCRAC 4 cut(s) 371, 627, 640, 654
MnlI CCTC 6 cut(s) 100, 169, 244, 623, 1034, 1096
Mox20I TGGCCA 1 cut(s) 415
MscI TGGCCA 1 cut(s) 415
MseI TTAA 5 cut(s) 309, 527, 747, 855, 1083
MslI CAYNNNNRTG 3 cut(s) 396, 409, 951
Msp20I TGGCCA 1 cut(s) 415
MspI CCGG 2 cut(s) 767, 1011
MspR9I CCNGG 2 cut(s) 206, 438
MunI CAATTG 1 cut(s) 417
MvaI CCWGG 2 cut(s) 206, 438
MwoI GCNNNNNNNGC 2 cut(s) 155, 553
NcoI CCATGG 2 cut(s) 818, 952
NdeII GATC 2 cut(s) 144, 331
NlaIII CATG 6 cut(s) 186, 232, 480, 822, 901, 956
NlaIV GGNNCC 7 cut(s) 24, 188, 198, 685, 725, 765, 816
NmeAIII GCCGAG 1 cut(s) 982
NmuCI GTSAC 2 cut(s) 497, 642
PagI TCATGA 1 cut(s) 897
PceI AGGCCT 1 cut(s) 919
PfeI GAWTC 2 cut(s) 671, 755
PfoI TCCNGGA 1 cut(s) 204
PinAI ACCGGT 1 cut(s) 1010
PkrI GCNGC 3 cut(s) 403, 814, 1071
Ppu21I YACGTR 1 cut(s) 463
PsiI TTATAA 1 cut(s) 804
Psp6I CCWGG 2 cut(s) 204, 436
PspGI CCWGG 2 cut(s) 204, 436
PspN4I GGNNCC 7 cut(s) 24, 188, 198, 685, 725, 765, 816
PspPI GGNCC 4 cut(s) 126, 186, 197, 575
RsaI GTAC 4 cut(s) 326, 662, 1025, 1116
RsaNI GTAC 4 cut(s) 325, 661, 1024, 1115
RseI CAYNNNNRTG 3 cut(s) 396, 409, 951
SaqAI TTAA 5 cut(s) 309, 527, 747, 855, 1083
SatI GCNGC 3 cut(s) 402, 813, 1070
Sau3AI GATC 2 cut(s) 144, 331
Sau96I GGNCC 4 cut(s) 126, 186, 197, 575
ScrFI CCNGG 2 cut(s) 206, 438
SduI GDGCHC 2 cut(s) 27, 712
SfaNI GCATC 1 cut(s) 388
SinI GGWCC 4 cut(s) 126, 186, 197, 575
SmiMI CAYNNNNRTG 3 cut(s) 396, 409, 951
SmlI CTYRAG 3 cut(s) 36, 876, 1132
SmoI CTYRAG 3 cut(s) 36, 876, 1132
Sse9I AATT 7 cut(s) 417, 441, 450, 469, 696, 852, 1050
SseBI AGGCCT 1 cut(s) 919
SsiI CCGC 4 cut(s) 78, 101, 727, 812
SspI AATATT 1 cut(s) 936
SspMI CTAG 5 cut(s) 357, 594, 668, 740, 1047
StuI AGGCCT 1 cut(s) 919
StyD4I CCNGG 2 cut(s) 204, 436
StyI CCWWGG 5 cut(s) 96, 368, 818, 927, 952
TaaI ACNGT 4 cut(s) 126, 138, 987, 1019
TaiI ACGT 2 cut(s) 465, 520
TaqI TCGA 2 cut(s) 243, 568
TasI AATT 7 cut(s) 417, 441, 450, 469, 696, 852, 1050
TatI WGTACW 3 cut(s) 324, 660, 1114
TauI GCSGC 1 cut(s) 815
TfiI GAWTC 2 cut(s) 671, 755
Tru1I TTAA 5 cut(s) 309, 527, 747, 855, 1083
Tru9I TTAA 5 cut(s) 309, 527, 747, 855, 1083
TscAI CASTG 3 cut(s) 136, 712, 1117
TseFI GTSAC 2 cut(s) 497, 642
TseI GCWGC 2 cut(s) 401, 1069
Tsp45I GTSAC 2 cut(s) 497, 642
TspDTI ATGAA 5 cut(s) 264, 420, 462, 545, 914
TspGWI ACGGA 1 cut(s) 1041
TspRI CASTG 3 cut(s) 136, 712, 1117
VpaK11BI GGWCC 4 cut(s) 126, 186, 197, 575
XapI RAATTY 2 cut(s) 441, 450
XbaI TCTAGA 1 cut(s) 739
XcmI CCANNNNNNNNNTGG 1 cut(s) 646
XmiI GTMKAC 1 cut(s) 829
XspI CTAG 5 cut(s) 357, 594, 668, 740, 1047
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.