FvH4_6g24730
NAC Family

inactive purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0007682
Physical Location & Seq
Reverse (-)
18730648 .. 18734988
4341 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g24730.t4

Sequence Viewer

Length: 1149 bp
ATGATGATGATGATGGGAAGAAGGCAGAGACTGTTGTGCTGCTTCACCATCTTTACATGTGTGTATCTCTACACCCTTGTCCTCGCTCTGCCAACCAAACCGGACGGCGATCACCGTCTGCTACGCCCCGGTGGCGAGCCTTTCAAGATCGCTCTCTTTGCCGACCTGCATTTCGGAGAGGCCGCCTGGACCGACTGGGGTCCACTCCAAGATGTTAACTCCGTCAGAGTCATGTCCACTGTGCTTGATCATGAATCCCCAGATTTTGTTATCTATCTTGGAGATGTTGTCACGGCCAACAATATAGCAATAAAAAATGCAAGCTTGTATTGGGATCAGGCAATTTCTCCAACAAGAGCCAGAGGAATACCTTGGGCCAGTGTTTTTGGAAACCATGATGATGCAGCATTTGAGTGGCCAATGGAGTGGTTTTCAGGTCCTGGAATTCCTGAACTTCGTTGCTCTGTTGCCAATTCCTCTTGCTCAGTAGGAGAAGAAGAGTGTAGCTTCAGAGGCACACAACGTCTGGAGTTAATGAAAAATGAGATTGTACTTAGCTCTCTTTCATACTCTCAATACGGTCCTAAAGAACTTTGGCCTAGTGTTTCCAACTATGTCCTCCAAGTTTCTTCATCAAACAATCCAGGGTCACCTGTGGCAGTTTTATATTTCCTAGATTCTGGTGGTGGTTCCTACCCAGAAGTTTTATCAAGTGCCCAAGCAGAATGGTTCCAGCAGAAAGCTTTAGAGATTAACCCTGATACAAGGGTGCCTGAGCTTATCTTTTGGCATATTCCAAGTCGTGCTTACAAGAAAGTGGCTCCAAGATTTGGTATACACAAGCCTTGTGTTGGTTCAATTAACAAGGAGAGAGTAGCAACTCAAGATGCTGAAACTGGTATCATGAAACTTCTGGCTGAAAGAGTTTCTGTCAAGGCAATATTTGTAGGACACAACCATGGATTGGACTGGTGCTGCCCTTATGAGAAACTGTGGCTATGCTTTGCTAGGCATACTGGTTATGGTGGCTATGGAAACTGGACTAGAGGAGCTAGAATTGTAGAAATCACCCAGAAGCCTTTCTCTATTAAATCTTGGATAAGAATGGAGGATGGTAGTGTTCGTAGTGAACTTGTCTTGAGTCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0005575 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

383

Amino Acids

42.84

Weight (kDa)

6.32

Isoelectric Point (pI)

32.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 48 - 177 1.6e-08 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 174
AccB1I GGYRCC 1 cut(s) 769
AccB7I CCANNNNNTGG 2 cut(s) 830, 964
AccI GTMKAC 1 cut(s) 835
AciI CCGC 1 cut(s) 183
AclWI GGATC 1 cut(s) 342
AcoI YGGCCR 2 cut(s) 294, 416
AcsI RAATTY 1 cut(s) 444
AcuI CTGAAG 1 cut(s) 493
AfaI GTAC 1 cut(s) 552
AfiI CCNNNNNNNGG 3 cut(s) 830, 851, 964
AflIII ACRYGT 1 cut(s) 56
AgsI TTSAA 2 cut(s) 145, 858
AjnI CCWGG 3 cut(s) 185, 439, 643
AluBI AGCT 6 cut(s) 324, 507, 558, 743, 778, 1052
AluI AGCT 6 cut(s) 324, 507, 558, 743, 778, 1052
Alw26I GTCTC 1 cut(s) 22
AlwI GGATC 1 cut(s) 342
AlwNI CAGNNNCTG 2 cut(s) 31, 440
AoxI GGCC 5 cut(s) 180, 294, 375, 416, 596
ApeKI GCWGC 3 cut(s) 39, 404, 975
ApoI RAATTY 1 cut(s) 444
ArsI GACNNNNNNTTYG 2 cut(s) 155, 187
Asp700I GAANNNNTTC 1 cut(s) 1079
AspS9I GGNCC 5 cut(s) 189, 200, 375, 437, 581
AsuC2I CCSGG 1 cut(s) 129
AsuHPI GGTGA 4 cut(s) 37, 104, 642, 1060
AvaII GGWCC 4 cut(s) 189, 200, 437, 581
BaeGI GKGCMC 1 cut(s) 718
BaeI ACNNNNGTAYC 2 cut(s) 753, 786
BalI TGGCCA 1 cut(s) 418
BanI GGYRCC 1 cut(s) 769
BbvI GCAGC 3 cut(s) 26, 416, 962
BccI CCATC 3 cut(s) 7, 56, 1106
BceAI ACGGC 2 cut(s) 121, 309
BciT130I CCWGG 3 cut(s) 187, 441, 645
BclI TGATCA 1 cut(s) 247
BcnI CCSGG 1 cut(s) 129
BcoDI GTCTC 1 cut(s) 22
BfaI CTAG 5 cut(s) 600, 674, 1008, 1044, 1053
BfuAI ACCTGC 1 cut(s) 174
BglI GCCNNNNNGGC 1 cut(s) 132
BisI GCNGC 4 cut(s) 40, 183, 405, 976
BlsI GCNGC 4 cut(s) 41, 184, 406, 977
Bme1390I CCNGG 4 cut(s) 129, 187, 441, 645
Bme18I GGWCC 4 cut(s) 189, 200, 437, 581
BmgT120I GGNCC 5 cut(s) 189, 200, 375, 437, 581
BmiI GGNNCC 5 cut(s) 201, 691, 731, 771, 822
BmrFI CCNGG 4 cut(s) 129, 187, 441, 645
BmrI ACTGGG 1 cut(s) 205
BmsI GCATC 2 cut(s) 391, 877
BmuI ACTGGG 1 cut(s) 205
BoxI GACNNNNGTC 1 cut(s) 198
BpmI CTGGAG 1 cut(s) 548
Bpu10I CCTNAGC 1 cut(s) 774
BpuEI CTTGAG 1 cut(s) 867
BpuMI CCSGG 1 cut(s) 129
BsaJI CCNNGG 4 cut(s) 127, 371, 644, 958
BsaWI WCCGGW 1 cut(s) 100
BsaXI ACNNNNNCTCC 2 cut(s) 273, 303
Bsc4I CCNNNNNNNGG 3 cut(s) 830, 851, 964
Bse1I ACTGG 6 cut(s) 200, 378, 901, 974, 1021, 1043
BseBI CCWGG 3 cut(s) 187, 441, 645
BseDI CCNNGG 4 cut(s) 127, 371, 644, 958
BseGI GGATG 1 cut(s) 1117
BseLI CCNNNNNNNGG 3 cut(s) 830, 851, 964
BseMII CTCAG 2 cut(s) 498, 765
BseNI ACTGG 6 cut(s) 200, 378, 901, 974, 1021, 1043
BseRI GAGGAG 1 cut(s) 1062
BseSI GKGCMC 1 cut(s) 718
BseXI GCAGC 3 cut(s) 26, 416, 962
BshFI GGCC 5 cut(s) 182, 296, 377, 418, 598
BshNI GGYRCC 1 cut(s) 769
BsiSI CCGG 2 cut(s) 101, 129
BslI CCNNNNNNNGG 3 cut(s) 830, 851, 964
BsmAI GTCTC 1 cut(s) 22
BsnI GGCC 5 cut(s) 182, 296, 377, 418, 598
Bsp1286I GDGCHC 1 cut(s) 718
Bsp143I GATC 4 cut(s) 109, 147, 247, 334
Bsp19I CCATGG 1 cut(s) 958
BspACI CCGC 1 cut(s) 183
BspANI GGCC 5 cut(s) 182, 296, 377, 418, 598
BspCNI CTCAG 2 cut(s) 497, 766
BspHI TCATGA 2 cut(s) 250, 903
BspLI GGNNCC 5 cut(s) 201, 691, 731, 771, 822
BspMI ACCTGC 1 cut(s) 174
BspPI GGATC 1 cut(s) 342
BspT107I GGYRCC 1 cut(s) 769
BsrI ACTGG 6 cut(s) 200, 378, 901, 974, 1021, 1043
BssECI CCNNGG 4 cut(s) 127, 371, 644, 958
BssMI GATC 4 cut(s) 109, 147, 247, 334
BssNAI GTATAC 1 cut(s) 836
BssT1I CCWWGG 2 cut(s) 371, 958
Bst1107I GTATAC 1 cut(s) 836
Bst2UI CCWGG 3 cut(s) 187, 441, 645
Bst4CI ACNGT 5 cut(s) 33, 116, 241, 581, 993
Bst6I CTCTTC 1 cut(s) 492
BstC8I GCNNGC 2 cut(s) 137, 322
BstDEI CTNAG 4 cut(s) 484, 554, 774, 1146
BstDSI CCRYGG 1 cut(s) 958
BstEII GGTNACC 1 cut(s) 648
BstF5I GGATG 1 cut(s) 1117
BstKTI GATC 4 cut(s) 112, 150, 250, 337
BstMAI GTCTC 1 cut(s) 22
BstMBI GATC 4 cut(s) 109, 147, 247, 334
BstMWI GCNNNNNNNGC 3 cut(s) 132, 158, 513
BstNI CCWGG 3 cut(s) 187, 441, 645
BstNSI RCATGY 1 cut(s) 60
BstPAI GACNNNNGTC 1 cut(s) 198
BstPI GGTNACC 1 cut(s) 648
BstSCI CCNGG 4 cut(s) 127, 185, 439, 643
BstSLI GKGCMC 1 cut(s) 718
BstV1I GCAGC 3 cut(s) 26, 416, 962
BstXI CCANNNNNNTGG 1 cut(s) 426
BstZ17I GTATAC 1 cut(s) 836
BsuRI GGCC 5 cut(s) 182, 296, 377, 418, 598
BtgI CCRYGG 1 cut(s) 958
BtsCI GGATG 1 cut(s) 1117
BtsIMutI CAGTG 2 cut(s) 237, 385
BveI ACCTGC 1 cut(s) 174
Cac8I GCNNGC 2 cut(s) 137, 322
CaiI CAGNNNCTG 2 cut(s) 31, 440
CciI TCATGA 2 cut(s) 250, 903
Cfr13I GGNCC 5 cut(s) 189, 200, 375, 437, 581
Csp6I GTAC 1 cut(s) 551
CspCI CAANNNNNGTGG 2 cut(s) 226, 261
CviAII CATG 6 cut(s) 57, 232, 251, 395, 904, 959
CviQI GTAC 1 cut(s) 551
DdeI CTNAG 4 cut(s) 484, 554, 774, 1146
DpnI GATC 4 cut(s) 111, 149, 249, 336
DpnII GATC 4 cut(s) 109, 147, 247, 334
EaeI YGGCCR 2 cut(s) 294, 416
Eam1104I CTCTTC 1 cut(s) 492
EarI CTCTTC 1 cut(s) 492
Eco130I CCWWGG 2 cut(s) 371, 958
Eco47I GGWCC 4 cut(s) 189, 200, 437, 581
Eco57I CTGAAG 1 cut(s) 493
Eco91I GGTNACC 1 cut(s) 648
EcoO109I RGGNCCY 1 cut(s) 437
EcoO65I GGTNACC 1 cut(s) 648
EcoRI GAATTC 1 cut(s) 444
EcoRII CCWGG 3 cut(s) 185, 439, 643
EcoT14I CCWWGG 2 cut(s) 371, 958
ErhI CCWWGG 2 cut(s) 371, 958
FaeI CATG 6 cut(s) 60, 235, 254, 398, 907, 962
FalI AAGNNNNNCTT 2 cut(s) 790, 822
FatI CATG 6 cut(s) 56, 231, 250, 394, 903, 958
FbaI TGATCA 1 cut(s) 247
FblI GTMKAC 1 cut(s) 835
Fnu4HI GCNGC 4 cut(s) 40, 183, 405, 976
FokI GGATG 1 cut(s) 1124
Fsp4HI GCNGC 4 cut(s) 40, 183, 405, 976
FspBI CTAG 5 cut(s) 600, 674, 1008, 1044, 1053
GluI GCNGC 4 cut(s) 40, 183, 405, 976
GsuI CTGGAG 1 cut(s) 548
HaeIII GGCC 5 cut(s) 182, 296, 377, 418, 598
HapII CCGG 2 cut(s) 101, 129
Hin1II CATG 6 cut(s) 60, 235, 254, 398, 907, 962
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HindIII AAGCTT 2 cut(s) 322, 741
HinfI GANTC 4 cut(s) 228, 254, 677, 1141
HpaI GTTAAC 1 cut(s) 217
HpaII CCGG 2 cut(s) 101, 129
HphI GGTGA 4 cut(s) 37, 104, 642, 1060
Hpy166II GTNNAC 5 cut(s) 203, 217, 237, 836, 1130
Hpy188I TCNGA 3 cut(s) 176, 227, 512
Hpy188III TCNNGA 7 cut(s) 145, 251, 449, 527, 884, 904, 1138
Hpy8I GTNNAC 5 cut(s) 203, 217, 237, 836, 1130
HpyAV CCTTC 1 cut(s) 15
HpyCH4III ACNGT 5 cut(s) 33, 116, 241, 581, 993
HpyCH4IV ACGT 1 cut(s) 523
HpyCH4V TGCA 3 cut(s) 169, 320, 404
HpyF10VI GCNNNNNNNGC 3 cut(s) 132, 158, 513
HpyF3I CTNAG 4 cut(s) 484, 554, 774, 1146
HpySE526I ACGT 1 cut(s) 523
Hsp92II CATG 6 cut(s) 60, 235, 254, 398, 907, 962
Ksp22I TGATCA 1 cut(s) 247
KspAI GTTAAC 1 cut(s) 217
Kzo9I GATC 4 cut(s) 109, 147, 247, 334
LmnI GCTCC 2 cut(s) 826, 1049
Lsp1109I GCAGC 3 cut(s) 26, 416, 962
LweI GCATC 2 cut(s) 391, 877
MaeI CTAG 5 cut(s) 600, 674, 1008, 1044, 1053
MaeII ACGT 1 cut(s) 523
MaeIII GTNAC 2 cut(s) 289, 648
MalI GATC 4 cut(s) 111, 149, 249, 336
MboI GATC 4 cut(s) 109, 147, 247, 334
MboII GAAGA 4 cut(s) 30, 506, 509, 621
MhlI GDGCHC 1 cut(s) 718
MlsI TGGCCA 1 cut(s) 418
MluCI AATT 5 cut(s) 342, 444, 472, 858, 1056
MluNI TGGCCA 1 cut(s) 418
MlyI GAGTC 1 cut(s) 237
MmeI TCCRAC 2 cut(s) 374, 633
MnlI CCTC 8 cut(s) 92, 172, 356, 487, 506, 629, 1040, 1102
Mox20I TGGCCA 1 cut(s) 418
MroXI GAANNNNTTC 1 cut(s) 1079
MscI TGGCCA 1 cut(s) 418
MseI TTAA 5 cut(s) 216, 533, 753, 861, 1089
MslI CAYNNNNRTG 3 cut(s) 399, 412, 957
Msp20I TGGCCA 1 cut(s) 418
MspI CCGG 2 cut(s) 101, 129
MspR9I CCNGG 4 cut(s) 129, 187, 441, 645
MvaI CCWGG 3 cut(s) 187, 441, 645
MwoI GCNNNNNNNGC 3 cut(s) 132, 158, 513
NciI CCSGG 1 cut(s) 129
NcoI CCATGG 1 cut(s) 958
NdeII GATC 4 cut(s) 109, 147, 247, 334
NlaIII CATG 6 cut(s) 60, 235, 254, 398, 907, 962
NlaIV GGNNCC 5 cut(s) 201, 691, 731, 771, 822
NmuCI GTSAC 2 cut(s) 289, 648
NspI RCATGY 1 cut(s) 60
PagI TCATGA 2 cut(s) 250, 903
PciI ACATGT 1 cut(s) 56
PdmI GAANNNNTTC 1 cut(s) 1079
PfeI GAWTC 2 cut(s) 254, 677
PflMI CCANNNNNTGG 2 cut(s) 830, 964
PfoI TCCNGGA 1 cut(s) 439
PkrI GCNGC 4 cut(s) 41, 184, 406, 977
PleI GAGTC 1 cut(s) 236
PpsI GAGTC 1 cut(s) 236
PpuMI RGGWCCY 1 cut(s) 437
PscI ACATGT 1 cut(s) 56
PshAI GACNNNNGTC 1 cut(s) 198
Psp5II RGGWCCY 1 cut(s) 437
Psp6I CCWGG 3 cut(s) 185, 439, 643
PspEI GGTNACC 1 cut(s) 648
PspGI CCWGG 3 cut(s) 185, 439, 643
PspN4I GGNNCC 5 cut(s) 201, 691, 731, 771, 822
PspPI GGNCC 5 cut(s) 189, 200, 375, 437, 581
PspPPI RGGWCCY 1 cut(s) 437
PstNI CAGNNNCTG 2 cut(s) 31, 440
RsaI GTAC 1 cut(s) 552
RsaNI GTAC 1 cut(s) 551
RseI CAYNNNNRTG 3 cut(s) 399, 412, 957
SaqAI TTAA 5 cut(s) 216, 533, 753, 861, 1089
SatI GCNGC 4 cut(s) 40, 183, 405, 976
Sau3AI GATC 4 cut(s) 109, 147, 247, 334
Sau96I GGNCC 5 cut(s) 189, 200, 375, 437, 581
SchI GAGTC 1 cut(s) 237
ScrFI CCNGG 4 cut(s) 129, 187, 441, 645
SduI GDGCHC 1 cut(s) 718
SfaNI GCATC 2 cut(s) 391, 877
SinI GGWCC 4 cut(s) 189, 200, 437, 581
SmiMI CAYNNNNRTG 3 cut(s) 399, 412, 957
SmlI CTYRAG 2 cut(s) 882, 1138
SmoI CTYRAG 2 cut(s) 882, 1138
Sse9I AATT 5 cut(s) 342, 444, 472, 858, 1056
SsiI CCGC 1 cut(s) 183
SspI AATATT 1 cut(s) 942
SspMI CTAG 5 cut(s) 600, 674, 1008, 1044, 1053
StyD4I CCNGG 4 cut(s) 127, 185, 439, 643
StyI CCWWGG 2 cut(s) 371, 958
TaaI ACNGT 5 cut(s) 33, 116, 241, 581, 993
TaiI ACGT 1 cut(s) 526
TaqII GACCGA 1 cut(s) 206
TasI AATT 5 cut(s) 342, 444, 472, 858, 1056
TatI WGTACW 1 cut(s) 550
TauI GCSGC 1 cut(s) 185
TfiI GAWTC 2 cut(s) 254, 677
Tru1I TTAA 5 cut(s) 216, 533, 753, 861, 1089
Tru9I TTAA 5 cut(s) 216, 533, 753, 861, 1089
TscAI CASTG 2 cut(s) 244, 385
TseFI GTSAC 2 cut(s) 289, 648
TseI GCWGC 3 cut(s) 39, 404, 975
Tsp45I GTSAC 2 cut(s) 289, 648
TspDTI ATGAA 5 cut(s) 267, 551, 555, 621, 920
TspGWI ACGGA 1 cut(s) 211
TspRI CASTG 2 cut(s) 244, 385
Van91I CCANNNNNTGG 2 cut(s) 830, 964
VpaK11BI GGWCC 4 cut(s) 189, 200, 437, 581
XapI RAATTY 1 cut(s) 444
XceI RCATGY 1 cut(s) 60
XmiI GTMKAC 1 cut(s) 835
XmnI GAANNNNTTC 1 cut(s) 1079
XspI CTAG 5 cut(s) 600, 674, 1008, 1044, 1053
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.