pycom14g06960
NAC Family

inactive purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0007682
Physical Location & Seq
Reverse (-)
6907941 .. 6914564
6624 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g06960.4

Sequence Viewer

Length: 915 bp
ATGTCCTCTGTACTCGATCATGAAACCCCAGAATTTGTTATCTATCTTGGAGATGTCATCACAGCTAATAATATATTAATTAGAAATGCAAGCTTGTATTGGGATCAGGCAATTTCTCCAACAAGAGCCAGGGGTATTCCTTGGGCCAGTGTGTTTGGGAACCATGATGATGCAGCATTTGAGTGGCCGATTGAGTGGTTTTCGGCTCCTGGAATTCCTAAAATTCATTGTCCTGTAGCCAATTCATCATGCTCAGGGAAAGAAGACTGTAGCTTCCAGGGAACTCAACGTTTGGAGCTAATGAAAAATGAGATTGAGCTTAATATGCTATCACATTCTAGATATGGCCCGAAGGGGCTTTGGCCTAGTGTATCCAACTATGTCCTCCAAGTTTTGTCATCAGAAGATCCCAAGTCAGCTGTGGCATTTTTGTACTTCCTAGATTCTGGTGGTGGTTCCTACCCAGAAGTTATATCCAGCGCCCAAGCAGAATGGTTCCGGCTGAAAGCTCTAGAGATTAACCCTGATTCAAGTGTCCCAGAGATAATCTTCTGGCATATACCAAGTCGTGCATATGAGAAAGTTGCTCCAAAGTTCGGCATACACAAGCCTTGCGTGGGTTCACTTAACGAAGAGCAAGTAGCACCTCAAGAAGCTGAAATGGGTATGATGAAACTTTTGGCCAAAAGGAAATCTGCCAAGGCAATATTTGTAGGACACAACCATGGCTTAGACTGGTGCTGTCCCCATAAAGAATTGTGGCTATGTTTTGCTAGGCATACCGGTTATGGCGGTTATGGAAACTGGGATAGAGGAGCTAGAATTGTGGAAATCACTCAAAAGCCTTTCTCCGTTAAATCTTGGATAAGAATGGAGGATGGCAATGTCCATAGTGAAGTTGTCTTGAGTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0005575 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

305

Amino Acids

34.04

Weight (kDa)

6.0

Isoelectric Point (pI)

41.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 792
AclI AACGTT 1 cut(s) 289
AclWI GGATC 2 cut(s) 111, 401
AcoI YGGCCR 2 cut(s) 185, 681
AcsI RAATTY 3 cut(s) 32, 213, 222
AfaI GTAC 2 cut(s) 12, 434
AfiI CCNNNNNNNGG 2 cut(s) 596, 617
AgeI ACCGGT 1 cut(s) 782
AgsI TTSAA 1 cut(s) 531
AjnI CCWGG 3 cut(s) 128, 208, 276
AluBI AGCT 9 cut(s) 65, 93, 273, 298, 319, 419, 509, 656, 818
AluI AGCT 9 cut(s) 65, 93, 273, 298, 319, 419, 509, 656, 818
AlwI GGATC 2 cut(s) 111, 401
AoxI GGCC 5 cut(s) 144, 185, 346, 362, 681
ApeKI GCWGC 1 cut(s) 173
ApoI RAATTY 3 cut(s) 32, 213, 222
AseI ATTAAT 1 cut(s) 77
AsiGI ACCGGT 1 cut(s) 782
AspLEI GCGC 1 cut(s) 482
AspS9I GGNCC 2 cut(s) 144, 347
BalI TGGCCA 1 cut(s) 683
BbsI GAAGAC 1 cut(s) 270
BbvI GCAGC 1 cut(s) 185
BccI CCATC 1 cut(s) 872
BciT130I CCWGG 3 cut(s) 130, 210, 278
BciVI GTATCC 1 cut(s) 382
BfaI CTAG 6 cut(s) 339, 366, 440, 512, 774, 819
BfmI CTRYAG 2 cut(s) 234, 268
BfoI RGCGCY 1 cut(s) 483
BfuI GTATCC 1 cut(s) 382
BisI GCNGC 1 cut(s) 174
BlsI GCNGC 1 cut(s) 175
Bme1390I CCNGG 3 cut(s) 130, 210, 278
BmgT120I GGNCC 2 cut(s) 144, 347
BmiI GGNNCC 4 cut(s) 161, 207, 457, 497
BmrFI CCNGG 3 cut(s) 130, 210, 278
BmrI ACTGGG 1 cut(s) 814
BmsI GCATC 1 cut(s) 160
BmuI ACTGGG 1 cut(s) 814
BpiI GAAGAC 1 cut(s) 270
Bpu10I CCTNAGC 1 cut(s) 253
BpuEI CTTGAG 1 cut(s) 633
BsaJI CCNNGG 5 cut(s) 129, 140, 277, 699, 724
BsaWI WCCGGW 1 cut(s) 782
Bsc4I CCNNNNNNNGG 2 cut(s) 596, 617
Bse118I RCCGGY 1 cut(s) 782
Bse1I ACTGG 3 cut(s) 147, 740, 809
Bse3DI GCAATG 1 cut(s) 889
BseBI CCWGG 3 cut(s) 130, 210, 278
BseDI CCNNGG 5 cut(s) 129, 140, 277, 699, 724
BseGI GGATG 1 cut(s) 883
BseLI CCNNNNNNNGG 2 cut(s) 596, 617
BseMI GCAATG 1 cut(s) 889
BseMII CTCAG 1 cut(s) 267
BseNI ACTGG 3 cut(s) 147, 740, 809
BseRI GAGGAG 1 cut(s) 828
BseXI GCAGC 1 cut(s) 185
BshFI GGCC 5 cut(s) 146, 187, 348, 364, 683
BshTI ACCGGT 1 cut(s) 782
BsiSI CCGG 2 cut(s) 499, 783
BslFI GGGAC 3 cut(s) 521, 729, 894
BslI CCNNNNNNNGG 2 cut(s) 596, 617
BsmFI GGGAC 3 cut(s) 521, 729, 894
BsnI GGCC 5 cut(s) 146, 187, 348, 364, 683
Bsp143I GATC 3 cut(s) 16, 103, 406
Bsp19I CCATGG 1 cut(s) 724
BspACI CCGC 1 cut(s) 792
BspANI GGCC 5 cut(s) 146, 187, 348, 364, 683
BspCNI CTCAG 1 cut(s) 266
BspHI TCATGA 1 cut(s) 19
BspLI GGNNCC 4 cut(s) 161, 207, 457, 497
BspPI GGATC 2 cut(s) 111, 401
BspQI GCTCTTC 1 cut(s) 627
BsrDI GCAATG 1 cut(s) 889
BsrFI RCCGGY 1 cut(s) 782
BsrI ACTGG 3 cut(s) 147, 740, 809
BssAI RCCGGY 1 cut(s) 782
BssECI CCNNGG 5 cut(s) 129, 140, 277, 699, 724
BssMI GATC 3 cut(s) 16, 103, 406
BssT1I CCWWGG 3 cut(s) 140, 699, 724
Bst2UI CCWGG 3 cut(s) 130, 210, 278
Bst4CI ACNGT 1 cut(s) 269
Bst6I CTCTTC 1 cut(s) 627
BstC8I GCNNGC 1 cut(s) 91
BstDEI CTNAG 2 cut(s) 253, 730
BstDSI CCRYGG 1 cut(s) 724
BstF5I GGATG 1 cut(s) 883
BstH2I RGCGCY 1 cut(s) 483
BstHHI GCGC 1 cut(s) 482
BstKTI GATC 3 cut(s) 19, 106, 409
BstMBI GATC 3 cut(s) 16, 103, 406
BstMWI GCNNNNNNNGC 1 cut(s) 325
BstNI CCWGG 3 cut(s) 130, 210, 278
BstSCI CCNGG 3 cut(s) 128, 208, 276
BstSFI CTRYAG 2 cut(s) 234, 268
BstV1I GCAGC 1 cut(s) 185
BstV2I GAAGAC 1 cut(s) 270
BstX2I RGATCY 1 cut(s) 406
BstYI RGATCY 1 cut(s) 406
BsuI GTATCC 1 cut(s) 382
BsuRI GGCC 5 cut(s) 146, 187, 348, 364, 683
BtgI CCRYGG 1 cut(s) 724
BtsCI GGATG 1 cut(s) 883
BtsIMutI CAGTG 1 cut(s) 154
Cac8I GCNNGC 1 cut(s) 91
CciI TCATGA 1 cut(s) 19
CfoI GCGC 1 cut(s) 482
Cfr10I RCCGGY 1 cut(s) 782
Cfr13I GGNCC 2 cut(s) 144, 347
Csp6I GTAC 2 cut(s) 11, 433
CspAI ACCGGT 1 cut(s) 782
CviAII CATG 4 cut(s) 20, 164, 249, 725
CviQI GTAC 2 cut(s) 11, 433
DdeI CTNAG 2 cut(s) 253, 730
DpnI GATC 3 cut(s) 18, 105, 408
DpnII GATC 3 cut(s) 16, 103, 406
EaeI YGGCCR 2 cut(s) 185, 681
Eam1104I CTCTTC 1 cut(s) 627
EarI CTCTTC 1 cut(s) 627
Eco130I CCWWGG 3 cut(s) 140, 699, 724
EcoRI GAATTC 1 cut(s) 213
EcoRII CCWGG 3 cut(s) 128, 208, 276
EcoT14I CCWWGG 3 cut(s) 140, 699, 724
ErhI CCWWGG 3 cut(s) 140, 699, 724
FaeI CATG 4 cut(s) 23, 167, 252, 728
FaqI GGGAC 3 cut(s) 521, 729, 894
FatI CATG 4 cut(s) 19, 163, 248, 724
FauNDI CATATG 1 cut(s) 574
Fnu4HI GCNGC 1 cut(s) 174
FokI GGATG 1 cut(s) 890
Fsp4HI GCNGC 1 cut(s) 174
FspBI CTAG 6 cut(s) 339, 366, 440, 512, 774, 819
GlaI GCGC 1 cut(s) 481
GluI GCNGC 1 cut(s) 174
HaeII RGCGCY 1 cut(s) 483
HaeIII GGCC 5 cut(s) 146, 187, 348, 364, 683
HapII CCGG 2 cut(s) 499, 783
HhaI GCGC 1 cut(s) 482
Hin1II CATG 4 cut(s) 23, 167, 252, 728
Hin6I GCGC 1 cut(s) 480
HinP1I GCGC 1 cut(s) 480
HindIII AAGCTT 1 cut(s) 91
HinfI GANTC 3 cut(s) 443, 527, 907
HpaII CCGG 2 cut(s) 499, 783
Hpy166II GTNNAC 1 cut(s) 623
Hpy188I TCNGA 1 cut(s) 403
Hpy188III TCNNGA 5 cut(s) 20, 339, 512, 650, 904
Hpy8I GTNNAC 1 cut(s) 623
HpyAV CCTTC 1 cut(s) 346
HpyCH4III ACNGT 1 cut(s) 269
HpyCH4IV ACGT 1 cut(s) 289
HpyCH4V TGCA 3 cut(s) 89, 173, 572
HpyF10VI GCNNNNNNNGC 1 cut(s) 325
HpyF3I CTNAG 2 cut(s) 253, 730
HpySE526I ACGT 1 cut(s) 289
Hsp92II CATG 4 cut(s) 23, 167, 252, 728
HspAI GCGC 1 cut(s) 480
Kzo9I GATC 3 cut(s) 16, 103, 406
LguI GCTCTTC 1 cut(s) 627
LmnI GCTCC 4 cut(s) 211, 295, 592, 815
Lsp1109I GCAGC 1 cut(s) 185
LweI GCATC 1 cut(s) 160
MaeI CTAG 6 cut(s) 339, 366, 440, 512, 774, 819
MaeII ACGT 1 cut(s) 289
MalI GATC 3 cut(s) 18, 105, 408
MboI GATC 3 cut(s) 16, 103, 406
MboII GAAGA 4 cut(s) 275, 416, 541, 644
MflI RGATCY 1 cut(s) 406
MlsI TGGCCA 1 cut(s) 683
MluCI AATT 8 cut(s) 32, 78, 111, 213, 222, 241, 755, 822
MluNI TGGCCA 1 cut(s) 683
MmeI TCCRAC 2 cut(s) 143, 399
MnlI CCTC 5 cut(s) 16, 395, 657, 806, 868
Mox20I TGGCCA 1 cut(s) 683
MscI TGGCCA 1 cut(s) 683
MseI TTAA 5 cut(s) 77, 321, 519, 627, 855
MslI CAYNNNNRTG 3 cut(s) 168, 181, 723
Msp20I TGGCCA 1 cut(s) 683
MspA1I CMGCKG 1 cut(s) 419
MspI CCGG 2 cut(s) 499, 783
MspR9I CCNGG 3 cut(s) 130, 210, 278
MvaI CCWGG 3 cut(s) 130, 210, 278
MwoI GCNNNNNNNGC 1 cut(s) 325
NcoI CCATGG 1 cut(s) 724
NdeI CATATG 1 cut(s) 574
NdeII GATC 3 cut(s) 16, 103, 406
NlaIII CATG 4 cut(s) 23, 167, 252, 728
NlaIV GGNNCC 4 cut(s) 161, 207, 457, 497
PagI TCATGA 1 cut(s) 19
PciSI GCTCTTC 1 cut(s) 627
PfeI GAWTC 2 cut(s) 443, 527
PfoI TCCNGGA 1 cut(s) 208
PinAI ACCGGT 1 cut(s) 782
PkrI GCNGC 1 cut(s) 175
PshBI ATTAAT 1 cut(s) 77
Psp1406I AACGTT 1 cut(s) 289
Psp6I CCWGG 3 cut(s) 128, 208, 276
PspGI CCWGG 3 cut(s) 128, 208, 276
PspN4I GGNNCC 4 cut(s) 161, 207, 457, 497
PspPI GGNCC 2 cut(s) 144, 347
PsuI RGATCY 1 cut(s) 406
PvuII CAGCTG 1 cut(s) 419
RsaI GTAC 2 cut(s) 12, 434
RsaNI GTAC 2 cut(s) 11, 433
RseI CAYNNNNRTG 3 cut(s) 168, 181, 723
SapI GCTCTTC 1 cut(s) 627
SaqAI TTAA 5 cut(s) 77, 321, 519, 627, 855
SatI GCNGC 1 cut(s) 174
Sau3AI GATC 3 cut(s) 16, 103, 406
Sau96I GGNCC 2 cut(s) 144, 347
ScrFI CCNGG 3 cut(s) 130, 210, 278
SfaNI GCATC 1 cut(s) 160
SfcI CTRYAG 2 cut(s) 234, 268
SmiMI CAYNNNNRTG 3 cut(s) 168, 181, 723
SmlI CTYRAG 2 cut(s) 648, 904
SmoI CTYRAG 2 cut(s) 648, 904
Sse9I AATT 8 cut(s) 32, 78, 111, 213, 222, 241, 755, 822
SsiI CCGC 1 cut(s) 792
SspI AATATT 1 cut(s) 708
SspMI CTAG 6 cut(s) 339, 366, 440, 512, 774, 819
StyD4I CCNGG 3 cut(s) 128, 208, 276
StyI CCWWGG 3 cut(s) 140, 699, 724
TaaI ACNGT 1 cut(s) 269
TaiI ACGT 1 cut(s) 292
TaqI TCGA 1 cut(s) 15
TasI AATT 8 cut(s) 32, 78, 111, 213, 222, 241, 755, 822
TatI WGTACW 2 cut(s) 10, 432
TfiI GAWTC 2 cut(s) 443, 527
Tru1I TTAA 5 cut(s) 77, 321, 519, 627, 855
Tru9I TTAA 5 cut(s) 77, 321, 519, 627, 855
TscAI CASTG 1 cut(s) 154
TseI GCWGC 1 cut(s) 173
TspDTI ATGAA 5 cut(s) 36, 215, 234, 317, 686
TspGWI ACGGA 1 cut(s) 841
TspRI CASTG 1 cut(s) 154
VspI ATTAAT 1 cut(s) 77
XapI RAATTY 3 cut(s) 32, 213, 222
XbaI TCTAGA 2 cut(s) 338, 511
XcmI CCANNNNNNNNNTGG 1 cut(s) 418
XspI CTAG 6 cut(s) 339, 366, 440, 512, 774, 819
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.