MD14G1080700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0009266
Physical Location & Seq
Forward (+)
9218752 .. 9229939
11188 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1080700.v1.1.491

Sequence Viewer

Length: 768 bp
ATGTTTTTAATAGTGTGCCCAAAAAAGGGGTGCCCATGCCAAGATTTCTACGTGATTAGCCCATATACTCTAGGCCTCCTCTGTTACTCTGCGCTTTCAAGCGCTTCCTGGTTTGCGACCGAGCTCTGCTCACACACACACGCACTGAGCTGTGATTTGGTGGGACTCAACAACATTTCATTTGCAGTGACAAAGCGCACAAAAATGGCAACCACCTCTCGCTCATTGTACCGCAGACTGTGCCTGCGGTCCTTCTCCACAAGCACAAAGCCAACGCATCACAACAGCCACCAGCAGAACCACCAGTACATGGAACCCAATTCCTTCATTGGGTGTTGGGAGGCCCCCAAGGACCCCAAGGAGGCGGAGGCCAAGCTTGCACACCTCCGCCGAGACTACACCAAGCAGGTCAAGGAGGTCCGCAAGGAGTACATCAAGGAGGTCGAGCTCATGAGGCTTGATAAGCTTCTCAAGGACGAGGCTCGTAAAGAAGCCCTTAGGCTCCAGAACGAGGAGCGCAAGAGGCTCAAGGCGGAGGCTGCAAAGGTCCGCGCCCAACAGCGTGAGGTTGCCGAGCAGGAGTTCCAGCAAACGCTGTTGAAAGAAAGAGCAGAGAAGCTTGAGAATTGGAAGATGAAGGAAAAACGGAAAGAAGAGATGAAAAAAGACGAGAAAGAACTGCTGCGACGCAAGAGTTCCATGTGGATCAATGAACAAGAACTGGAAAAGAAGATTTTAGAAGCCATCGTTGATACCACACCCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

256

Amino Acids

30.0

Weight (kDa)

9.27

Isoelectric Point (pI)

48.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 397
AccB1I GGYRCC 1 cut(s) 30
AccB7I CCANNNNNTGG 1 cut(s) 310
AccII CGCG 1 cut(s) 552
AciI CCGC 7 cut(s) 232, 247, 365, 388, 421, 533, 550
AclWI GGATC 1 cut(s) 713
AfaI GTAC 3 cut(s) 230, 308, 431
AfeI AGCGCT 1 cut(s) 103
AfiI CCNNNNNNNGG 6 cut(s) 25, 26, 310, 330, 361, 511
AgsI TTSAA 2 cut(s) 99, 601
AjnI CCWGG 1 cut(s) 107
AluBI AGCT 6 cut(s) 124, 150, 376, 448, 466, 619
AluI AGCT 6 cut(s) 124, 150, 376, 448, 466, 619
Alw21I GWGCWC 2 cut(s) 126, 450
Alw26I GTCTC 1 cut(s) 387
AlwI GGATC 1 cut(s) 713
Aor51HI AGCGCT 1 cut(s) 103
AoxI GGCC 3 cut(s) 73, 342, 369
ApeKI GCWGC 2 cut(s) 539, 682
AspLEI GCGC 5 cut(s) 94, 104, 198, 519, 554
AspS9I GGNCC 5 cut(s) 249, 343, 352, 418, 547
AvaII GGWCC 4 cut(s) 249, 352, 418, 547
AxyI CCTNAGG 1 cut(s) 497
BaeGI GKGCMC 2 cut(s) 20, 35
BanI GGYRCC 1 cut(s) 30
BanII GRGCYC 2 cut(s) 126, 450
Bbv12I GWGCWC 2 cut(s) 126, 450
BbvI GCAGC 2 cut(s) 526, 669
BccI CCATC 1 cut(s) 752
BciT130I CCWGG 1 cut(s) 109
BcoDI GTCTC 1 cut(s) 387
BfaI CTAG 1 cut(s) 71
BfoI RGCGCY 1 cut(s) 105
BfuAI ACCTGC 1 cut(s) 397
BisI GCNGC 2 cut(s) 540, 683
BlsI GCNGC 2 cut(s) 541, 684
Bme1390I CCNGG 1 cut(s) 109
Bme18I GGWCC 4 cut(s) 249, 352, 418, 547
BmgT120I GGNCC 5 cut(s) 249, 343, 352, 418, 547
BmiI GGNNCC 5 cut(s) 32, 315, 345, 354, 503
BmrFI CCNGG 1 cut(s) 109
BmsI GCATC 1 cut(s) 286
BplI GAGNNNNNCTC 2 cut(s) 113, 145
BpmI CTGGAG 1 cut(s) 488
BpuEI CTTGAG 3 cut(s) 455, 512, 641
BsaAI YACGTR 1 cut(s) 52
BsaJI CCNNGG 2 cut(s) 348, 357
Bsc4I CCNNNNNNNGG 6 cut(s) 25, 26, 310, 330, 361, 511
Bse1I ACTGG 2 cut(s) 304, 726
Bse21I CCTNAGG 1 cut(s) 497
BseBI CCWGG 1 cut(s) 109
BseDI CCNNGG 2 cut(s) 348, 357
BseLI CCNNNNNNNGG 6 cut(s) 25, 26, 310, 330, 361, 511
BseMII CTCAG 1 cut(s) 137
BseNI ACTGG 2 cut(s) 304, 726
BseRI GAGGAG 2 cut(s) 68, 527
BseSI GKGCMC 2 cut(s) 20, 35
BseXI GCAGC 2 cut(s) 526, 669
Bsh1236I CGCG 1 cut(s) 552
Bsh1285I CGRYCG 1 cut(s) 120
BshFI GGCC 3 cut(s) 75, 344, 371
BshNI GGYRCC 1 cut(s) 30
BsiEI CGRYCG 1 cut(s) 120
BsiHKAI GWGCWC 2 cut(s) 126, 450
BslFI GGGAC 1 cut(s) 177
BslI CCNNNNNNNGG 6 cut(s) 25, 26, 310, 330, 361, 511
BsmAI GTCTC 1 cut(s) 387
BsmFI GGGAC 1 cut(s) 177
BsnI GGCC 3 cut(s) 75, 344, 371
Bsp1286I GDGCHC 4 cut(s) 20, 35, 126, 450
Bsp143I GATC 1 cut(s) 705
BspACI CCGC 7 cut(s) 232, 247, 365, 388, 421, 533, 550
BspANI GGCC 3 cut(s) 75, 344, 371
BspCNI CTCAG 1 cut(s) 138
BspFNI CGCG 1 cut(s) 552
BspHI TCATGA 1 cut(s) 450
BspLI GGNNCC 5 cut(s) 32, 315, 345, 354, 503
BspMI ACCTGC 1 cut(s) 397
BspPI GGATC 1 cut(s) 713
BspT107I GGYRCC 1 cut(s) 30
BsrI ACTGG 2 cut(s) 304, 726
BssECI CCNNGG 2 cut(s) 348, 357
BssMI GATC 1 cut(s) 705
BssT1I CCWWGG 2 cut(s) 348, 357
Bst2UI CCWGG 1 cut(s) 109
Bst4CI ACNGT 1 cut(s) 240
Bst6I CTCTTC 1 cut(s) 648
BstAPI GCANNNNNTGC 1 cut(s) 240
BstBAI YACGTR 1 cut(s) 52
BstC8I GCNNGC 2 cut(s) 245, 378
BstDEI CTNAG 2 cut(s) 146, 497
BstFNI CGCG 1 cut(s) 552
BstH2I RGCGCY 1 cut(s) 105
BstHHI GCGC 5 cut(s) 94, 104, 198, 519, 554
BstKTI GATC 1 cut(s) 708
BstMAI GTCTC 1 cut(s) 387
BstMBI GATC 1 cut(s) 705
BstMCI CGRYCG 1 cut(s) 120
BstMWI GCNNNNNNNGC 6 cut(s) 240, 377, 454, 463, 523, 539
BstNI CCWGG 1 cut(s) 109
BstSCI CCNGG 1 cut(s) 107
BstSLI GKGCMC 2 cut(s) 20, 35
BstUI CGCG 1 cut(s) 552
BstV1I GCAGC 2 cut(s) 526, 669
Bsu36I CCTNAGG 1 cut(s) 497
BsuRI GGCC 3 cut(s) 75, 344, 371
BtsI GCAGTG 1 cut(s) 192
BtsIMutI CAGTG 2 cut(s) 143, 192
BveI ACCTGC 1 cut(s) 397
Cac8I GCNNGC 2 cut(s) 245, 378
CciI TCATGA 1 cut(s) 450
CfoI GCGC 5 cut(s) 94, 104, 198, 519, 554
Cfr13I GGNCC 5 cut(s) 249, 343, 352, 418, 547
CseI GACGC 1 cut(s) 696
Csp6I GTAC 3 cut(s) 229, 307, 430
CviAII CATG 4 cut(s) 36, 310, 451, 700
CviQI GTAC 3 cut(s) 229, 307, 430
DdeI CTNAG 2 cut(s) 146, 497
DpnI GATC 1 cut(s) 707
DpnII GATC 1 cut(s) 705
Eam1104I CTCTTC 1 cut(s) 648
EarI CTCTTC 1 cut(s) 648
EciI GGCGGA 3 cut(s) 377, 380, 548
Ecl136II GAGCTC 2 cut(s) 124, 448
Eco130I CCWWGG 2 cut(s) 348, 357
Eco147I AGGCCT 1 cut(s) 75
Eco24I GRGCYC 2 cut(s) 126, 450
Eco47I GGWCC 4 cut(s) 249, 352, 418, 547
Eco47III AGCGCT 1 cut(s) 103
Eco53kI GAGCTC 2 cut(s) 124, 448
Eco81I CCTNAGG 1 cut(s) 497
EcoICRI GAGCTC 2 cut(s) 124, 448
EcoO109I RGGNCCY 2 cut(s) 343, 352
EcoRII CCWGG 1 cut(s) 107
EcoT14I CCWWGG 2 cut(s) 348, 357
EcoT38I GRGCYC 2 cut(s) 126, 450
ErhI CCWWGG 2 cut(s) 348, 357
FaeI CATG 4 cut(s) 39, 313, 454, 703
FaiI YATR 6 cut(s) 37, 64, 66, 311, 452, 701
FalI AAGNNNNNCTT 2 cut(s) 480, 512
FaqI GGGAC 1 cut(s) 177
FatI CATG 4 cut(s) 35, 309, 450, 699
Fnu4HI GCNGC 2 cut(s) 540, 683
FriOI GRGCYC 2 cut(s) 126, 450
Fsp4HI GCNGC 2 cut(s) 540, 683
FspBI CTAG 1 cut(s) 71
GlaI GCGC 5 cut(s) 93, 103, 197, 518, 553
GluI GCNGC 2 cut(s) 540, 683
GsuI CTGGAG 1 cut(s) 488
HaeII RGCGCY 1 cut(s) 105
HaeIII GGCC 3 cut(s) 75, 344, 371
HgaI GACGC 1 cut(s) 696
HhaI GCGC 5 cut(s) 94, 104, 198, 519, 554
Hin1II CATG 4 cut(s) 39, 313, 454, 703
Hin6I GCGC 5 cut(s) 92, 102, 196, 517, 552
HinP1I GCGC 5 cut(s) 92, 102, 196, 517, 552
HindIII AAGCTT 3 cut(s) 374, 464, 617
HinfI GANTC 1 cut(s) 165
Hpy188III TCNNGA 2 cut(s) 451, 505
Hpy99I CGWCG 1 cut(s) 690
HpyAV CCTTC 3 cut(s) 262, 334, 631
HpyCH4III ACNGT 1 cut(s) 240
HpyCH4IV ACGT 1 cut(s) 51
HpyCH4V TGCA 3 cut(s) 185, 380, 542
HpyF10VI GCNNNNNNNGC 6 cut(s) 240, 377, 454, 463, 523, 539
HpyF3I CTNAG 2 cut(s) 146, 497
HpySE526I ACGT 1 cut(s) 51
Hsp92II CATG 4 cut(s) 39, 313, 454, 703
HspAI GCGC 5 cut(s) 92, 102, 196, 517, 552
Kzo9I GATC 1 cut(s) 705
LmnI GCTCC 2 cut(s) 507, 514
Lsp1109I GCAGC 2 cut(s) 526, 669
LweI GCATC 1 cut(s) 286
MaeI CTAG 1 cut(s) 71
MaeII ACGT 1 cut(s) 51
MaeIII GTNAC 2 cut(s) 83, 187
MalI GATC 1 cut(s) 707
MboI GATC 1 cut(s) 705
MboII GAAGA 3 cut(s) 643, 665, 742
MhlI GDGCHC 4 cut(s) 20, 35, 126, 450
MluCI AATT 2 cut(s) 319, 625
MlyI GAGTC 1 cut(s) 159
MseI TTAA 1 cut(s) 8
MslI CAYNNNNRTG 1 cut(s) 203
MspR9I CCNGG 1 cut(s) 109
MvaI CCWGG 1 cut(s) 109
MvnI CGCG 1 cut(s) 552
MwoI GCNNNNNNNGC 6 cut(s) 240, 377, 454, 463, 523, 539
NdeII GATC 1 cut(s) 705
NlaIII CATG 4 cut(s) 39, 313, 454, 703
NlaIV GGNNCC 5 cut(s) 32, 315, 345, 354, 503
NmeAIII GCCGAG 2 cut(s) 416, 598
NmuCI GTSAC 1 cut(s) 187
PagI TCATGA 1 cut(s) 450
PceI AGGCCT 1 cut(s) 75
PflMI CCANNNNNTGG 1 cut(s) 310
PkrI GCNGC 2 cut(s) 541, 684
PleI GAGTC 1 cut(s) 159
PpsI GAGTC 1 cut(s) 159
Ppu21I YACGTR 1 cut(s) 52
PpuMI RGGWCCY 1 cut(s) 352
Psp124BI GAGCTC 2 cut(s) 126, 450
Psp5II RGGWCCY 1 cut(s) 352
Psp6I CCWGG 1 cut(s) 107
PspGI CCWGG 1 cut(s) 107
PspN4I GGNNCC 5 cut(s) 32, 315, 345, 354, 503
PspPI GGNCC 5 cut(s) 249, 343, 352, 418, 547
PspPPI RGGWCCY 1 cut(s) 352
PsrI GAACNNNNNNTAC 2 cut(s) 290, 322
RsaI GTAC 3 cut(s) 230, 308, 431
RsaNI GTAC 3 cut(s) 229, 307, 430
RseI CAYNNNNRTG 1 cut(s) 203
SacI GAGCTC 2 cut(s) 126, 450
SaqAI TTAA 1 cut(s) 8
SatI GCNGC 2 cut(s) 540, 683
Sau3AI GATC 1 cut(s) 705
Sau96I GGNCC 5 cut(s) 249, 343, 352, 418, 547
SchI GAGTC 1 cut(s) 159
ScrFI CCNGG 1 cut(s) 109
SduI GDGCHC 4 cut(s) 20, 35, 126, 450
SfaNI GCATC 1 cut(s) 286
SinI GGWCC 4 cut(s) 249, 352, 418, 547
SmiMI CAYNNNNRTG 1 cut(s) 203
SmlI CTYRAG 3 cut(s) 470, 527, 620
SmoI CTYRAG 3 cut(s) 470, 527, 620
Sse9I AATT 2 cut(s) 319, 625
SseBI AGGCCT 1 cut(s) 75
SsiI CCGC 7 cut(s) 232, 247, 365, 388, 421, 533, 550
SspMI CTAG 1 cut(s) 71
SstI GAGCTC 2 cut(s) 126, 450
StuI AGGCCT 1 cut(s) 75
StyD4I CCNGG 1 cut(s) 107
StyI CCWWGG 2 cut(s) 348, 357
TaaI ACNGT 1 cut(s) 240
TaiI ACGT 1 cut(s) 54
TaqI TCGA 1 cut(s) 444
TaqII GACCGA 1 cut(s) 134
TasI AATT 2 cut(s) 319, 625
TatI WGTACW 2 cut(s) 306, 429
Tru1I TTAA 1 cut(s) 8
Tru9I TTAA 1 cut(s) 8
TscAI CASTG 2 cut(s) 150, 192
TseFI GTSAC 1 cut(s) 187
TseI GCWGC 2 cut(s) 539, 682
Tsp45I GTSAC 1 cut(s) 187
TspDTI ATGAA 5 cut(s) 168, 316, 650, 674, 726
TspGWI ACGGA 1 cut(s) 661
TspRI CASTG 2 cut(s) 150, 192
Van91I CCANNNNNTGG 1 cut(s) 310
VpaK11BI GGWCC 4 cut(s) 249, 352, 418, 547
XspI CTAG 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.