MD14G1123700.v1.1
NAC Family

inactive receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0015660
Physical Location & Seq
Reverse (-)
19857397 .. 19859187
1791 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1123700.v1.1.491

Sequence Viewer

Length: 1035 bp
ATGCTAACCCTAAGAGCAAAACCTAGAAATAAGAGCCCCAAACATGACGACCTAATAAAAAGCTACGCCAGCTCCTTCGCCGACTATGAAGACTGCATCGTCGGTTTCATGGACGATCTGCCACTTGTCTTCTGTGGGCACGAGAAGAAGTGTTTGGGTTGCGGGGAAAGGCTTGCCCACCTGACACTGAGGAGTGTGCTGAGAGGTTCGGTGGGTGTGATCGGAGAGAGCAAGCTGGGGATGACTGAGAAGGTGGTGCTGTTGGGAGGTAGGGTTTGTGCCGTGAAGAGGTTCAGGAATGTGAGCTCTGGGAAGCGTGAGTTTGGGAAGAGAATTGAGCACTTGGCTCAAGTGAGTCAGAAGTGTGAGTATCTTCTGCCTGTCATTGCTTATTTGTACGCTAAGAGGATCAAGTTCGTTCTCTCTGATTATAAACCCATGGGAAGCCTCGCCGACTTGCTTGCCGGTGCTAGGCAGCATGGCCACACAGCATTGGAATGGAACCAAAGGCTCACCATAGTTAATCATATTACACGAGCAATTGCTTTCATCCACGGACAATATCCTCCATACGAGAAGAAGATGAAGATGGACGTACATGGGAGCATTAGGCCATGCAATGTCATGGTGAACATGGACTTCTCGGCCTGCTTGTCGGATTACGGATTCACCCAATTGGCGCAGCCGGTAGAAAGTTCAAACACATGGCAGCTGATGAAGTCGCCCAGCTGGCAACTGGAAAATAGTTTCTGTGATGAACTGAGTCAGAAGAGTGACATTTACAATTTTGGGGTGATACTGCTGGACATATTGGCAGAACCAATGGGTTTGGGAATGACAAGAGGCATAAATGAAAAGCAATCAAACATGAAATTAGAAGGTGGTTTGGAGTTTTTTGAGTTTCCAGTGAAGGAAGGGAAAGAGAGGAGGCAAGTGTCAATGGTTTTGCATATTGCTTTGGCATGTACAAGTGCCAAACCAGAGGCTAGGCCTTCAATAGAGGAGATAGCTTTTAATATCTCGAAGATATTGTAA

Protein Analysis

345

Amino Acids

38.65

Weight (kDa)

8.89

Isoelectric Point (pI)

45.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 73 - 336 4.6e-10 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 90 - 337 8.8e-13 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015660)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g26430
malus_domestica MD06G1104800.v1.1 MD14G1123700.v1.1
prunus_persica Prupe.5G117400_v2.0.a1
pyrus_communis pycom06g09930
rosa_chinensis RchiOBHm_Chr7g0195111
rosa_laevigata RLG00000004145
rosa_multiflora Rmu_co8510033.1_g000001 Rmu_sc0002381.1_g000031
rosa_roxburghii Rroxscaffold_3G00260770
rosa_rugosa Rorug07G0020900
rosa_samantha Rh7AG148200 Rh7BG149000 Rh7CG152400 Rh7DG149300
rosa_wichuraiana Rw7G012650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 432
AasI GACNNNNNNGTC 1 cut(s) 98
AciI CCGC 1 cut(s) 162
AclWI GGATC 1 cut(s) 416
AcoI YGGCCR 1 cut(s) 481
AfaI GTAC 3 cut(s) 398, 597, 967
AfiI CCNNNNNNNGG 2 cut(s) 288, 471
AgsI TTSAA 2 cut(s) 699, 996
AjuI GAANNNNNNNTTGG 2 cut(s) 137, 169
AluBI AGCT 7 cut(s) 63, 72, 235, 306, 712, 729, 1010
AluI AGCT 7 cut(s) 63, 72, 235, 306, 712, 729, 1010
Alw21I GWGCWC 2 cut(s) 308, 342
AlwI GGATC 1 cut(s) 416
AoxI GGCC 4 cut(s) 481, 611, 645, 989
ApeKI GCWGC 3 cut(s) 475, 682, 709
Asp700I GAANNNNTTC 1 cut(s) 290
AspLEI GCGC 1 cut(s) 682
AsuHPI GGTGA 4 cut(s) 505, 640, 661, 805
BaeGI GKGCMC 1 cut(s) 141
BalI TGGCCA 1 cut(s) 483
BanII GRGCYC 2 cut(s) 38, 308
BauI CACGAG 2 cut(s) 140, 534
BbsI GAAGAC 2 cut(s) 96, 121
Bbv12I GWGCWC 2 cut(s) 308, 342
BbvI GCAGC 3 cut(s) 487, 694, 721
BccI CCATC 1 cut(s) 583
BceAI ACGGC 1 cut(s) 266
BcgI CGANNNNNNTGC 2 cut(s) 443, 477
BfaI CTAG 3 cut(s) 24, 471, 987
BglI GCCNNNNNGGC 1 cut(s) 730
BisI GCNGC 3 cut(s) 476, 683, 710
BlsI GCNGC 3 cut(s) 477, 684, 711
BmiI GGNNCC 1 cut(s) 503
BmsI GCATC 1 cut(s) 105
BpiI GAAGAC 2 cut(s) 96, 121
BpuEI CTTGAG 1 cut(s) 333
BsaJI CCNNGG 2 cut(s) 438, 553
BsaXI ACNNNNNCTCC 4 cut(s) 56, 86, 258, 288
Bsc4I CCNNNNNNNGG 2 cut(s) 288, 471
Bse118I RCCGGY 2 cut(s) 464, 685
Bse1I ACTGG 2 cut(s) 741, 905
Bse3DI GCAATG 2 cut(s) 384, 625
BseDI CCNNGG 2 cut(s) 438, 553
BseGI GGATG 2 cut(s) 246, 549
BseLI CCNNNNNNNGG 2 cut(s) 288, 471
BseMI GCAATG 2 cut(s) 384, 625
BseMII CTCAG 4 cut(s) 179, 191, 237, 752
BseNI ACTGG 2 cut(s) 741, 905
BseRI GAGGAG 3 cut(s) 205, 940, 1016
BseSI GKGCMC 1 cut(s) 141
BseXI GCAGC 3 cut(s) 487, 694, 721
BseYI CCCAGC 2 cut(s) 235, 725
BshFI GGCC 4 cut(s) 483, 613, 647, 991
BsiHKAI GWGCWC 2 cut(s) 308, 342
BsiSI CCGG 2 cut(s) 465, 686
BslI CCNNNNNNNGG 2 cut(s) 288, 471
BsnI GGCC 4 cut(s) 483, 613, 647, 991
Bsp1286I GDGCHC 4 cut(s) 38, 141, 308, 342
Bsp1407I TGTACA 1 cut(s) 965
Bsp143I GATC 3 cut(s) 115, 219, 408
Bsp19I CCATGG 1 cut(s) 438
BspACI CCGC 1 cut(s) 162
BspANI GGCC 4 cut(s) 483, 613, 647, 991
BspCNI CTCAG 4 cut(s) 180, 192, 238, 753
BspLI GGNNCC 1 cut(s) 503
BspPI GGATC 1 cut(s) 416
BsrDI GCAATG 2 cut(s) 384, 625
BsrFI RCCGGY 2 cut(s) 464, 685
BsrGI TGTACA 1 cut(s) 965
BsrI ACTGG 2 cut(s) 741, 905
BssAI RCCGGY 2 cut(s) 464, 685
BssECI CCNNGG 2 cut(s) 438, 553
BssMI GATC 3 cut(s) 115, 219, 408
BssSI CACGAG 2 cut(s) 140, 534
BssT1I CCWWGG 1 cut(s) 438
Bst2BI CACGAG 2 cut(s) 140, 534
Bst6I CTCTTC 3 cut(s) 281, 323, 764
BstAUI TGTACA 1 cut(s) 965
BstC8I GCNNGC 6 cut(s) 70, 174, 233, 462, 649, 731
BstDEI CTNAG 6 cut(s) 11, 188, 200, 246, 402, 761
BstDSI CCRYGG 2 cut(s) 438, 553
BstF5I GGATG 2 cut(s) 246, 549
BstHHI GCGC 1 cut(s) 682
BstKTI GATC 3 cut(s) 118, 222, 411
BstMBI GATC 3 cut(s) 115, 219, 408
BstMWI GCNNNNNNNGC 2 cut(s) 69, 730
BstNSI RCATGY 1 cut(s) 966
BstSLI GKGCMC 1 cut(s) 141
BstV1I GCAGC 3 cut(s) 487, 694, 721
BstV2I GAAGAC 2 cut(s) 96, 121
BsuRI GGCC 4 cut(s) 483, 613, 647, 991
BtgI CCRYGG 2 cut(s) 438, 553
BtsCI GGATG 2 cut(s) 246, 549
BtsIMutI CAGTG 2 cut(s) 185, 912
Cac8I GCNNGC 6 cut(s) 70, 174, 233, 462, 649, 731
CfoI GCGC 1 cut(s) 682
Cfr10I RCCGGY 2 cut(s) 464, 685
Csp6I GTAC 3 cut(s) 397, 596, 966
CviQI GTAC 3 cut(s) 397, 596, 966
DdeI CTNAG 6 cut(s) 11, 188, 200, 246, 402, 761
DpnI GATC 3 cut(s) 117, 221, 410
DpnII GATC 3 cut(s) 115, 219, 408
DrdI GACNNNNNNGTC 1 cut(s) 98
DseDI GACNNNNNNGTC 1 cut(s) 98
EaeI YGGCCR 1 cut(s) 481
Eam1104I CTCTTC 3 cut(s) 281, 323, 764
EarI CTCTTC 3 cut(s) 281, 323, 764
Ecl136II GAGCTC 1 cut(s) 306
Eco130I CCWWGG 1 cut(s) 438
Eco147I AGGCCT 1 cut(s) 991
Eco24I GRGCYC 2 cut(s) 38, 308
Eco53kI GAGCTC 1 cut(s) 306
EcoICRI GAGCTC 1 cut(s) 306
EcoT14I CCWWGG 1 cut(s) 438
EcoT38I GRGCYC 2 cut(s) 38, 308
ErhI CCWWGG 1 cut(s) 438
FauI CCCGC 1 cut(s) 155
Fnu4HI GCNGC 3 cut(s) 476, 683, 710
FokI GGATG 2 cut(s) 253, 536
FriOI GRGCYC 2 cut(s) 38, 308
Fsp4HI GCNGC 3 cut(s) 476, 683, 710
FspBI CTAG 3 cut(s) 24, 471, 987
GlaI GCGC 1 cut(s) 681
GluI GCNGC 3 cut(s) 476, 683, 710
GsaI CCCAGC 2 cut(s) 239, 729
HaeIII GGCC 4 cut(s) 483, 613, 647, 991
HapII CCGG 2 cut(s) 465, 686
HhaI GCGC 1 cut(s) 682
Hin6I GCGC 1 cut(s) 680
HinP1I GCGC 1 cut(s) 680
HinfI GANTC 3 cut(s) 355, 666, 763
HpaII CCGG 2 cut(s) 465, 686
HphI GGTGA 4 cut(s) 505, 640, 661, 805
Hpy166II GTNNAC 1 cut(s) 631
Hpy188I TCNGA 5 cut(s) 224, 360, 427, 658, 768
Hpy188III TCNNGA 2 cut(s) 295, 1021
Hpy8I GTNNAC 1 cut(s) 631
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 6 cut(s) 85, 244, 872, 904, 908, 1002
HpyCH4IV ACGT 1 cut(s) 594
HpyCH4V TGCA 3 cut(s) 96, 618, 949
HpyF10VI GCNNNNNNNGC 2 cut(s) 69, 730
HpyF3I CTNAG 6 cut(s) 11, 188, 200, 246, 402, 761
HpySE526I ACGT 1 cut(s) 594
HspAI GCGC 1 cut(s) 680
Kzo9I GATC 3 cut(s) 115, 219, 408
LmnI GCTCC 2 cut(s) 77, 603
Lsp1109I GCAGC 3 cut(s) 487, 694, 721
LweI GCATC 1 cut(s) 105
MaeI CTAG 3 cut(s) 24, 471, 987
MaeII ACGT 1 cut(s) 594
MaeIII GTNAC 1 cut(s) 773
MalI GATC 3 cut(s) 117, 221, 410
MboI GATC 3 cut(s) 115, 219, 408
MfeI CAATTG 2 cut(s) 540, 674
MhlI GDGCHC 4 cut(s) 38, 141, 308, 342
MlsI TGGCCA 1 cut(s) 483
MluCI AATT 5 cut(s) 333, 540, 674, 784, 872
MluNI TGGCCA 1 cut(s) 483
MlyI GAGTC 2 cut(s) 364, 772
MmeI TCCRAC 1 cut(s) 636
Mox20I TGGCCA 1 cut(s) 483
MroXI GAANNNNTTC 1 cut(s) 290
MscI TGGCCA 1 cut(s) 483
MseI TTAA 2 cut(s) 522, 1014
MslI CAYNNNNRTG 1 cut(s) 496
Msp20I TGGCCA 1 cut(s) 483
MspA1I CMGCKG 2 cut(s) 712, 729
MspI CCGG 2 cut(s) 465, 686
MunI CAATTG 2 cut(s) 540, 674
MwoI GCNNNNNNNGC 2 cut(s) 69, 730
NcoI CCATGG 1 cut(s) 438
NdeII GATC 3 cut(s) 115, 219, 408
NlaIV GGNNCC 1 cut(s) 503
NmeAIII GCCGAG 1 cut(s) 623
NmuCI GTSAC 1 cut(s) 773
NspI RCATGY 1 cut(s) 966
PceI AGGCCT 1 cut(s) 991
PdmI GAANNNNTTC 1 cut(s) 290
PfeI GAWTC 1 cut(s) 666
PkrI GCNGC 3 cut(s) 477, 684, 711
PleI GAGTC 2 cut(s) 363, 771
PpsI GAGTC 2 cut(s) 363, 771
PsiI TTATAA 1 cut(s) 432
Psp124BI GAGCTC 1 cut(s) 308
PspFI CCCAGC 2 cut(s) 235, 725
PspN4I GGNNCC 1 cut(s) 503
PvuII CAGCTG 2 cut(s) 712, 729
RsaI GTAC 3 cut(s) 398, 597, 967
RsaNI GTAC 3 cut(s) 397, 596, 966
RseI CAYNNNNRTG 1 cut(s) 496
SacI GAGCTC 1 cut(s) 308
SaqAI TTAA 2 cut(s) 522, 1014
SatI GCNGC 3 cut(s) 476, 683, 710
Sau3AI GATC 3 cut(s) 115, 219, 408
SchI GAGTC 2 cut(s) 364, 772
SduI GDGCHC 4 cut(s) 38, 141, 308, 342
SfaNI GCATC 1 cut(s) 105
SmiMI CAYNNNNRTG 1 cut(s) 496
SmlI CTYRAG 1 cut(s) 348
SmoI CTYRAG 1 cut(s) 348
Sse9I AATT 5 cut(s) 333, 540, 674, 784, 872
SseBI AGGCCT 1 cut(s) 991
SsiI CCGC 1 cut(s) 162
SspMI CTAG 3 cut(s) 24, 471, 987
SstI GAGCTC 1 cut(s) 308
StuI AGGCCT 1 cut(s) 991
StyI CCWWGG 1 cut(s) 438
TaiI ACGT 1 cut(s) 597
TaqI TCGA 1 cut(s) 1022
TasI AATT 5 cut(s) 333, 540, 674, 784, 872
TatI WGTACW 1 cut(s) 965
TfiI GAWTC 1 cut(s) 666
Tru1I TTAA 2 cut(s) 522, 1014
Tru9I TTAA 2 cut(s) 522, 1014
TscAI CASTG 2 cut(s) 192, 912
TseFI GTSAC 1 cut(s) 773
TseI GCWGC 3 cut(s) 475, 682, 709
Tsp45I GTSAC 1 cut(s) 773
TspDTI ATGAA 8 cut(s) 97, 102, 538, 599, 731, 771, 867, 884
TspGWI ACGGA 2 cut(s) 570, 678
TspRI CASTG 2 cut(s) 192, 912
XceI RCATGY 1 cut(s) 966
XcmI CCANNNNNNNNNTGG 1 cut(s) 733
XmnI GAANNNNTTC 1 cut(s) 290
XspI CTAG 3 cut(s) 24, 471, 987
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.