MD14G1125000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0014055
Physical Location & Seq
Forward (+)
20024753 .. 20026219
1467 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1125000.v1.1.491

Sequence Viewer

Length: 969 bp
ATGCCCTTAACTATCAAGCTCACTTGGGCTCCCTCCACAAATCCCACATCGCATCTCCTCCCCTCAAAACTCCTCACCCCATCTGAATCCTCCTCTGCAATCTTCTTCCCCCGAGCTTCTACTCCTTCCCCACCTCATCGACCTCTCATAGCAAAATGCACGACTGGTGGCGCTGCCGGAGAGGGCGGCGGCTTGAAGAAGGCACTGAGTGGCATTGTGGGAGAGCAAGTGGAGGAGCTCTTGAAAAGAGAAGAGAACAGGGAGTTGCTCGATGGGTTGGAAAAGGCGTCTCAGAGAGTTGAGATTGCTAAGAGAGAGCTGGCTGAGATTGAAAGGCAGGAACTTGAGGCCAAGCTTGTGAGGGATTACATTGCTCAACTAGAAAGCAGAGCTTCTGAGATTGCAGAATGTCAAAAGGAGATATCAGAAGCAAAATCCATGGTTGAAGAAGCTGAACGTGCCCTCTCACAAGACGGAGATCAACTCGGCGGCGGATATTCTTTTGCAGCGACCGAAAATAAGGAAATCGACAAGGATAAAGAGAGATGGCAATCCATAAAAGCAGCCTCAGTTTCCGCCCTAGTCGGCACTGTTGCTGCATTACCCTTCTCCTTCACTCAGGTGGCAAGCATTTCTGAGCTGATCCTCCCTCTGGGAGTCACCTTCGCCAGCTGTGCTCTATTCGGAGTCACCTTCCGATACACAATACGAAGAGACTTGGACGATGCTCATCTCAAGACCGGAGCGCCTGCAGCATTCGGAGTTGTCAAAGGGCTTGCTACACTGGATGGCGGACAGCCTTTGGAACTGAATGCCGGTAGCTTCTTTTCACATGCTTTTGATGGCGCGATTTACGTGTCTCAGAGTCTTTTTATTTTTCTTTCTGCTGCTGTTGCTCTGGATTATTGTTTTAAGACAGGGCTATTGAGTCCTTTTCCTATCAAGAACTCTCTATCAGGATCCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

323

Amino Acids

34.48

Weight (kDa)

5.34

Isoelectric Point (pI)

46.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014055)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24090
fragaria_vesca FvH4_5g11010
malus_domestica MD06G1117600.v1.1 MD14G1125000.v1.1
prunus_persica Prupe.5G128700_v2.0.a1
pyrus_communis pycom14g11050
rosa_chinensis RchiOBHm_Chr7g0186801
rosa_laevigata RLG00000004814
rosa_multiflora Rmu_co8237483.1_g000001 Rmu_ssc0000024.1_g000042
rosa_roxburghii Rroxscaffold_3G00267810
rosa_rugosa Rorug06G0476800
rosa_samantha Rh7AG063700 Rh7BG080200 Rh7CG083800 Rh7DG084700
rosa_wichuraiana Rw7G007270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 848
AciI CCGC 6 cut(s) 186, 189, 489, 492, 576, 792
AclWI GGATC 3 cut(s) 637, 954, 967
AcyI GRCGYC 1 cut(s) 287
AdeI CACNNNGTG 1 cut(s) 209
AfiI CCNNNNNNNGG 1 cut(s) 652
AflIII ACRYGT 1 cut(s) 855
AgsI TTSAA 4 cut(s) 196, 244, 332, 446
AleI CACNNNNGTG 1 cut(s) 620
Alw21I GWGCWC 2 cut(s) 240, 679
Alw26I GTCTC 3 cut(s) 294, 708, 864
AlwI GGATC 3 cut(s) 637, 954, 967
Ama87I CYCGRG 1 cut(s) 111
AoxI GGCC 1 cut(s) 348
ApeKI GCWGC 6 cut(s) 173, 506, 563, 596, 752, 887
AspLEI GCGC 3 cut(s) 173, 748, 848
AsuHPI GGTGA 3 cut(s) 67, 652, 682
AvaI CYCGRG 1 cut(s) 111
BaeGI GKGCMC 1 cut(s) 463
BamHI GGATCC 1 cut(s) 959
BanII GRGCYC 2 cut(s) 31, 240
Bbv12I GWGCWC 2 cut(s) 240, 679
BbvI GCAGC 6 cut(s) 160, 518, 575, 583, 764, 874
BccI CCATC 5 cut(s) 88, 266, 540, 782, 836
BcoDI GTCTC 3 cut(s) 294, 708, 864
BfaI CTAG 2 cut(s) 380, 581
BfmI CTRYAG 1 cut(s) 750
BfoI RGCGCY 2 cut(s) 174, 749
BisI GCNGC 9 cut(s) 174, 187, 190, 490, 507, 564, 597, 753, 888
BlsI GCNGC 9 cut(s) 175, 188, 191, 491, 508, 565, 598, 754, 889
BmeT110I CYCGRG 1 cut(s) 111
BmiI GGNNCC 2 cut(s) 30, 961
BmsI GCATC 2 cut(s) 61, 715
BplI GAGNNNNNCTC 2 cut(s) 468, 500
BpuEI CTTGAG 2 cut(s) 365, 719
BsaAI YACGTR 1 cut(s) 856
BsaBI GATNNNNATC 2 cut(s) 550, 729
BsaHI GRCGYC 1 cut(s) 287
BsaJI CCNNGG 1 cut(s) 438
BsaWI WCCGGW 1 cut(s) 740
BsaXI ACNNNNNCTCC 4 cut(s) 13, 43, 213, 243
Bsc4I CCNNNNNNNGG 1 cut(s) 652
Bse118I RCCGGY 1 cut(s) 815
Bse1I ACTGG 2 cut(s) 169, 789
Bse3DI GCAATG 1 cut(s) 369
Bse8I GATNNNNATC 2 cut(s) 550, 729
BseDI CCNNGG 1 cut(s) 438
BseGI GGATG 1 cut(s) 793
BseJI GATNNNNATC 2 cut(s) 550, 729
BseLI CCNNNNNNNGG 1 cut(s) 652
BseMI GCAATG 1 cut(s) 369
BseMII CTCAG 8 cut(s) 197, 305, 315, 387, 582, 627, 632, 875
BseNI ACTGG 2 cut(s) 169, 789
BseRI GAGGAG 4 cut(s) 47, 62, 82, 248
BseSI GKGCMC 1 cut(s) 463
BseXI GCAGC 6 cut(s) 160, 518, 575, 583, 764, 874
Bsh1236I CGCG 1 cut(s) 848
Bsh1285I CGRYCG 1 cut(s) 513
BshFI GGCC 1 cut(s) 350
BsiEI CGRYCG 1 cut(s) 513
BsiHKAI GWGCWC 2 cut(s) 240, 679
BsiHKCI CYCGRG 1 cut(s) 111
BsiSI CCGG 3 cut(s) 177, 741, 816
BslI CCNNNNNNNGG 1 cut(s) 652
BsmAI GTCTC 3 cut(s) 294, 708, 864
BsmBI CGTCTC 1 cut(s) 294
BsmI GAATGC 2 cut(s) 755, 817
BsnI GGCC 1 cut(s) 350
BsoBI CYCGRG 1 cut(s) 111
Bsp1286I GDGCHC 4 cut(s) 31, 240, 463, 679
Bsp143I GATC 3 cut(s) 478, 642, 959
Bsp19I CCATGG 1 cut(s) 438
BspACI CCGC 6 cut(s) 186, 189, 489, 492, 576, 792
BspANI GGCC 1 cut(s) 350
BspCNI CTCAG 8 cut(s) 198, 304, 316, 388, 581, 628, 631, 874
BspFNI CGCG 1 cut(s) 848
BspLI GGNNCC 2 cut(s) 30, 961
BspMAI CTGCAG 1 cut(s) 754
BspPI GGATC 3 cut(s) 637, 954, 967
BsrDI GCAATG 1 cut(s) 369
BsrFI RCCGGY 1 cut(s) 815
BsrI ACTGG 2 cut(s) 169, 789
BssAI RCCGGY 1 cut(s) 815
BssECI CCNNGG 1 cut(s) 438
BssMI GATC 3 cut(s) 478, 642, 959
BssNI GRCGYC 1 cut(s) 287
BssT1I CCWWGG 1 cut(s) 438
Bst4CI ACNGT 1 cut(s) 592
Bst6I CTCTTC 2 cut(s) 246, 706
BstACI GRCGYC 1 cut(s) 287
BstBAI YACGTR 1 cut(s) 856
BstC8I GCNNGC 5 cut(s) 321, 628, 670, 750, 777
BstDEI CTNAG 9 cut(s) 206, 291, 309, 324, 396, 568, 618, 636, 861
BstDSI CCRYGG 1 cut(s) 438
BstF5I GGATG 1 cut(s) 793
BstFNI CGCG 1 cut(s) 848
BstH2I RGCGCY 2 cut(s) 174, 749
BstHHI GCGC 3 cut(s) 173, 748, 848
BstKTI GATC 3 cut(s) 481, 645, 962
BstMAI GTCTC 3 cut(s) 294, 708, 864
BstMBI GATC 3 cut(s) 478, 642, 959
BstMCI CGRYCG 1 cut(s) 513
BstMWI GCNNNNNNNGC 4 cut(s) 458, 674, 752, 893
BstNSI RCATGY 1 cut(s) 836
BstSFI CTRYAG 1 cut(s) 750
BstSLI GKGCMC 1 cut(s) 463
BstUI CGCG 1 cut(s) 848
BstV1I GCAGC 6 cut(s) 160, 518, 575, 583, 764, 874
BstX2I RGATCY 1 cut(s) 959
BstYI RGATCY 1 cut(s) 959
BsuRI GGCC 1 cut(s) 350
BtgI CCRYGG 1 cut(s) 438
BtgZI GCGATG 1 cut(s) 33
BtsCI GGATG 1 cut(s) 793
BtsIMutI CAGTG 3 cut(s) 203, 588, 782
Cac8I GCNNGC 5 cut(s) 321, 628, 670, 750, 777
CfoI GCGC 3 cut(s) 173, 748, 848
Cfr10I RCCGGY 1 cut(s) 815
CseI GACGC 1 cut(s) 276
CviAII CATG 2 cut(s) 439, 833
DdeI CTNAG 9 cut(s) 206, 291, 309, 324, 396, 568, 618, 636, 861
DpnI GATC 3 cut(s) 480, 644, 961
DpnII GATC 3 cut(s) 478, 642, 959
DraIII CACNNNGTG 1 cut(s) 209
Eam1104I CTCTTC 2 cut(s) 246, 706
EarI CTCTTC 2 cut(s) 246, 706
EciI GGCGGA 3 cut(s) 507, 565, 807
Ecl136II GAGCTC 1 cut(s) 238
Eco130I CCWWGG 1 cut(s) 438
Eco24I GRGCYC 2 cut(s) 31, 240
Eco32I GATATC 1 cut(s) 423
Eco53kI GAGCTC 1 cut(s) 238
Eco88I CYCGRG 1 cut(s) 111
EcoICRI GAGCTC 1 cut(s) 238
EcoRV GATATC 1 cut(s) 423
EcoT14I CCWWGG 1 cut(s) 438
EcoT38I GRGCYC 2 cut(s) 31, 240
ErhI CCWWGG 1 cut(s) 438
Esp3I CGTCTC 1 cut(s) 294
FaeI CATG 2 cut(s) 442, 836
FaiI YATR 4 cut(s) 149, 440, 557, 834
FalI AAGNNNNNCTT 2 cut(s) 376, 408
FatI CATG 2 cut(s) 438, 832
Fnu4HI GCNGC 9 cut(s) 174, 187, 190, 490, 507, 564, 597, 753, 888
FokI GGATG 1 cut(s) 800
FriOI GRGCYC 2 cut(s) 31, 240
Fsp4HI GCNGC 9 cut(s) 174, 187, 190, 490, 507, 564, 597, 753, 888
FspBI CTAG 2 cut(s) 380, 581
GlaI GCGC 3 cut(s) 172, 747, 847
GluI GCNGC 9 cut(s) 174, 187, 190, 490, 507, 564, 597, 753, 888
HaeII RGCGCY 2 cut(s) 174, 749
HaeIII GGCC 1 cut(s) 350
HapII CCGG 3 cut(s) 177, 741, 816
HgaI GACGC 1 cut(s) 276
HhaI GCGC 3 cut(s) 173, 748, 848
Hin1I GRCGYC 1 cut(s) 287
Hin1II CATG 2 cut(s) 442, 836
Hin6I GCGC 3 cut(s) 171, 746, 846
HinP1I GCGC 3 cut(s) 171, 746, 846
HindIII AAGCTT 1 cut(s) 353
HinfI GANTC 5 cut(s) 86, 657, 687, 865, 928
HpaII CCGG 3 cut(s) 177, 741, 816
HphI GGTGA 3 cut(s) 67, 652, 682
Hpy188I TCNGA 9 cut(s) 85, 294, 397, 427, 637, 686, 698, 761, 864
Hpy188III TCNNGA 5 cut(s) 241, 736, 899, 943, 957
HpyAV CCTTC 6 cut(s) 135, 193, 616, 622, 673, 703
HpyCH4III ACNGT 1 cut(s) 592
HpyCH4IV ACGT 2 cut(s) 457, 855
HpyCH4V TGCA 6 cut(s) 98, 159, 404, 506, 599, 752
HpyF10VI GCNNNNNNNGC 4 cut(s) 458, 674, 752, 893
HpyF3I CTNAG 9 cut(s) 206, 291, 309, 324, 396, 568, 618, 636, 861
HpySE526I ACGT 2 cut(s) 457, 855
Hsp92I GRCGYC 1 cut(s) 287
Hsp92II CATG 2 cut(s) 442, 836
HspAI GCGC 3 cut(s) 171, 746, 846
Kzo9I GATC 3 cut(s) 478, 642, 959
LmnI GCTCC 3 cut(s) 34, 235, 743
Lsp1109I GCAGC 6 cut(s) 160, 518, 575, 583, 764, 874
LweI GCATC 2 cut(s) 61, 715
MaeI CTAG 2 cut(s) 380, 581
MaeII ACGT 2 cut(s) 457, 855
MaeIII GTNAC 2 cut(s) 658, 688
MalI GATC 3 cut(s) 480, 644, 961
MboI GATC 3 cut(s) 478, 642, 959
MboII GAAGA 6 cut(s) 94, 97, 208, 263, 458, 723
MflI RGATCY 1 cut(s) 959
MhlI GDGCHC 4 cut(s) 31, 240, 463, 679
MluCI AATT 1 cut(s) 964
MlyI GAGTC 4 cut(s) 666, 696, 874, 937
MmeI TCCRAC 1 cut(s) 258
MseI TTAA 3 cut(s) 8, 912, 967
MslI CAYNNNNRTG 1 cut(s) 620
MspA1I CMGCKG 1 cut(s) 672
MspI CCGG 3 cut(s) 177, 741, 816
Mva1269I GAATGC 2 cut(s) 755, 817
MvnI CGCG 1 cut(s) 848
MwoI GCNNNNNNNGC 4 cut(s) 458, 674, 752, 893
NcoI CCATGG 1 cut(s) 438
NdeII GATC 3 cut(s) 478, 642, 959
NlaIII CATG 2 cut(s) 442, 836
NlaIV GGNNCC 2 cut(s) 30, 961
NmeAIII GCCGAG 1 cut(s) 465
NmuCI GTSAC 2 cut(s) 658, 688
NspI RCATGY 1 cut(s) 836
OliI CACNNNNGTG 1 cut(s) 620
PctI GAATGC 2 cut(s) 755, 817
PfeI GAWTC 1 cut(s) 86
PkrI GCNGC 9 cut(s) 175, 188, 191, 491, 508, 565, 598, 754, 889
PleI GAGTC 4 cut(s) 665, 695, 873, 936
PpsI GAGTC 4 cut(s) 665, 695, 873, 936
Ppu21I YACGTR 1 cut(s) 856
Psp124BI GAGCTC 1 cut(s) 240
PspN4I GGNNCC 2 cut(s) 30, 961
PstI CTGCAG 1 cut(s) 754
PsuI RGATCY 1 cut(s) 959
PvuII CAGCTG 1 cut(s) 672
RseI CAYNNNNRTG 1 cut(s) 620
SacI GAGCTC 1 cut(s) 240
SaqAI TTAA 3 cut(s) 8, 912, 967
SatI GCNGC 9 cut(s) 174, 187, 190, 490, 507, 564, 597, 753, 888
Sau3AI GATC 3 cut(s) 478, 642, 959
SchI GAGTC 4 cut(s) 666, 696, 874, 937
SduI GDGCHC 4 cut(s) 31, 240, 463, 679
SfaNI GCATC 2 cut(s) 61, 715
SfcI CTRYAG 1 cut(s) 750
SmiMI CAYNNNNRTG 1 cut(s) 620
SmlI CTYRAG 2 cut(s) 344, 734
SmoI CTYRAG 2 cut(s) 344, 734
Sse9I AATT 1 cut(s) 964
SsiI CCGC 6 cut(s) 186, 189, 489, 492, 576, 792
SspMI CTAG 2 cut(s) 380, 581
SstI GAGCTC 1 cut(s) 240
StyI CCWWGG 1 cut(s) 438
TaaI ACNGT 1 cut(s) 592
TaiI ACGT 2 cut(s) 460, 858
TaqI TCGA 3 cut(s) 139, 270, 528
TaqII GACCGA 1 cut(s) 527
TasI AATT 1 cut(s) 964
TauI GCSGC 3 cut(s) 189, 192, 492
TfiI GAWTC 1 cut(s) 86
Tru1I TTAA 3 cut(s) 8, 912, 967
Tru9I TTAA 3 cut(s) 8, 912, 967
TscAI CASTG 3 cut(s) 210, 595, 789
TseFI GTSAC 2 cut(s) 658, 688
TseI GCWGC 6 cut(s) 173, 506, 563, 596, 752, 887
Tsp45I GTSAC 2 cut(s) 658, 688
TspGWI ACGGA 1 cut(s) 489
TspRI CASTG 3 cut(s) 210, 595, 789
XceI RCATGY 1 cut(s) 836
XspI CTAG 2 cut(s) 380, 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.