MD14G1144300.v1.1

Germin-like protein subfamily 3 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0016129
Physical Location & Seq
Reverse (-)
23752565 .. 23753242
678 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1144300.v1.1.491

Sequence Viewer

Length: 678 bp
ATGAGTTCGTATTTTTCCTGCTGCATTATTTTTCTCTCTGTCGTCTGTTTCTGCACCAAAATCTGTTCTGCAGACTCAGATAACCTCCAGGACACTTGCCCTACGTCCCCGCCAGGAAAGCAAACCATCTTCATCAATGGCTTCCCATGCAAGAATCCAAACAGCATCGCTGCCCCCGACTTCAAAACCTCCAAGCTAACCGAACCTGGCAACACAGACAATTTCTTTGGCTCCTCAGTGACTCTAGTCACTGCTGCAGACTTTCCAGGCCTCAACACGCTTGGCCTCTCAATTGCGCGAACAGACTTGAGAATAGATGGCATGGTGAGCCTCCACTCCCACCCTCGGGCATCCGAACTGTTCTTTGTGAGCAAAGGAATTGTACTTGCTGGGTTTATTGACACCAAAAACCAGCCATTCCAACAGGTTCTTGCAGAAGGAGATGTGTTTGTTTTCCCAAAGGGTTTGCTTCATTTTTCTCTGAATTTTGGGTACGATTTCGCCACTGTTTTCTCAGTTTTCAACAGCCAGAATCCAGGGGTTGTGGAAATTTCTGGTGCCATATTTCAATCTGATCTGGATATGATTGACAGTATGACAAAGGGGTTTCTCACTCATCACATCGGAAATCTCACTCTTAACAAGCAGCAATTCAGAAAGTTAAATCCTATCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

226

Amino Acids

24.53

Weight (kDa)

5.9

Isoelectric Point (pI)

29.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 65 - 191 2.8e-34 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016129)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G61750
fragaria_vesca FvH4_5g09990
malus_domestica MD14G1144300.v1.1
prunus_persica Prupe.5G138500_v2.0.a1
pyrus_communis pycom14g11990
rosa_chinensis RchiOBHm_Chr7g0188271
rosa_laevigata RLG00000004712
rosa_multiflora Rmu_sc0008750.1_g000009
rosa_roxburghii Rroxscaffold_3G00266540
rosa_rugosa Rorug06G0489600
rosa_samantha Rh7AG094000 Rh7BG095400 Rh7CG095800 Rh7DG096000
rosa_wichuraiana Rw7G008100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 557
AccII CGCG 1 cut(s) 298
AciI CCGC 1 cut(s) 110
AcsI RAATTY 2 cut(s) 484, 549
AfaI GTAC 2 cut(s) 384, 494
AfiI CCNNNNNNNGG 2 cut(s) 345, 346
AgsI TTSAA 3 cut(s) 184, 523, 569
AjnI CCWGG 5 cut(s) 87, 112, 205, 265, 535
AluBI AGCT 1 cut(s) 196
AluI AGCT 1 cut(s) 196
Ama87I CYCGRG 1 cut(s) 345
AoxI GGCC 2 cut(s) 268, 283
ApeKI GCWGC 4 cut(s) 21, 170, 254, 646
ApoI RAATTY 2 cut(s) 484, 549
ArsI GACNNNNNNTTYG 2 cut(s) 209, 241
AspLEI GCGC 1 cut(s) 298
AsuHPI GGTGA 1 cut(s) 337
AvaI CYCGRG 1 cut(s) 345
BanI GGYRCC 1 cut(s) 557
BbvI GCAGC 4 cut(s) 8, 157, 241, 658
BccI CCATC 2 cut(s) 134, 311
BciT130I CCWGG 5 cut(s) 89, 114, 207, 267, 537
BfaI CTAG 1 cut(s) 245
BfmI CTRYAG 2 cut(s) 69, 255
BisI GCNGC 4 cut(s) 22, 171, 255, 647
BlsI GCNGC 4 cut(s) 23, 172, 256, 648
Bme1390I CCNGG 5 cut(s) 89, 114, 207, 267, 537
BmeT110I CYCGRG 1 cut(s) 345
BmiI GGNNCC 2 cut(s) 232, 559
BmrFI CCNGG 5 cut(s) 89, 114, 207, 267, 537
BmsI GCATC 2 cut(s) 174, 359
BoxI GACNNNNGTC 1 cut(s) 245
BpmI CTGGAG 1 cut(s) 71
BpuEI CTTGAG 1 cut(s) 328
BsaJI CCNNGG 2 cut(s) 344, 536
Bsc4I CCNNNNNNNGG 2 cut(s) 345, 346
BseBI CCWGG 5 cut(s) 89, 114, 207, 267, 537
BseDI CCNNGG 2 cut(s) 344, 536
BseGI GGATG 1 cut(s) 350
BseLI CCNNNNNNNGG 2 cut(s) 345, 346
BseMII CTCAG 3 cut(s) 90, 249, 528
BseRI GAGGAG 1 cut(s) 223
BseXI GCAGC 4 cut(s) 8, 157, 241, 658
BseYI CCCAGC 1 cut(s) 389
BsgI GTGCAG 1 cut(s) 37
Bsh1236I CGCG 1 cut(s) 298
BshFI GGCC 2 cut(s) 270, 285
BshNI GGYRCC 1 cut(s) 557
BsiHKCI CYCGRG 1 cut(s) 345
BslFI GGGAC 1 cut(s) 91
BslI CCNNNNNNNGG 2 cut(s) 345, 346
BsmFI GGGAC 1 cut(s) 91
BsnI GGCC 2 cut(s) 270, 285
BsoBI CYCGRG 1 cut(s) 345
Bsp143I GATC 1 cut(s) 574
BspACI CCGC 1 cut(s) 110
BspANI GGCC 2 cut(s) 270, 285
BspCNI CTCAG 3 cut(s) 89, 248, 527
BspFNI CGCG 1 cut(s) 298
BspLI GGNNCC 2 cut(s) 232, 559
BspMAI CTGCAG 2 cut(s) 73, 259
BspT107I GGYRCC 1 cut(s) 557
BssECI CCNNGG 2 cut(s) 344, 536
BssMI GATC 1 cut(s) 574
Bst2UI CCWGG 5 cut(s) 89, 114, 207, 267, 537
Bst4CI ACNGT 3 cut(s) 360, 508, 593
BstDEI CTNAG 3 cut(s) 76, 235, 514
BstF5I GGATG 1 cut(s) 350
BstFNI CGCG 1 cut(s) 298
BstHHI GCGC 1 cut(s) 298
BstKTI GATC 1 cut(s) 577
BstMBI GATC 1 cut(s) 574
BstMWI GCNNNNNNNGC 3 cut(s) 118, 147, 327
BstNI CCWGG 5 cut(s) 89, 114, 207, 267, 537
BstPAI GACNNNNGTC 1 cut(s) 245
BstSCI CCNGG 5 cut(s) 87, 112, 205, 265, 535
BstSFI CTRYAG 2 cut(s) 69, 255
BstUI CGCG 1 cut(s) 298
BstV1I GCAGC 4 cut(s) 8, 157, 241, 658
BsuRI GGCC 2 cut(s) 270, 285
BtgZI GCGATG 1 cut(s) 151
BtsCI GGATG 1 cut(s) 350
BtsI GCAGTG 1 cut(s) 249
BtsIMutI CAGTG 3 cut(s) 243, 249, 504
CfoI GCGC 1 cut(s) 298
Csp6I GTAC 2 cut(s) 383, 493
CviAII CATG 2 cut(s) 147, 322
CviJI RGCY 8 cut(s) 141, 196, 231, 270, 285, 330, 415, 528
CviKI_1 RGCY 8 cut(s) 141, 196, 231, 270, 285, 330, 415, 528
CviQI GTAC 2 cut(s) 383, 493
DdeI CTNAG 3 cut(s) 76, 235, 514
DpnI GATC 1 cut(s) 576
DpnII GATC 1 cut(s) 574
Eco147I AGGCCT 1 cut(s) 270
Eco88I CYCGRG 1 cut(s) 345
EcoRII CCWGG 5 cut(s) 87, 112, 205, 265, 535
FaeI CATG 2 cut(s) 150, 325
FaiI YATR 7 cut(s) 148, 323, 563, 584, 596, 674, 676
FaqI GGGAC 1 cut(s) 91
FatI CATG 2 cut(s) 146, 321
FauI CCCGC 1 cut(s) 117
FauNDI CATATG 1 cut(s) 674
Fnu4HI GCNGC 4 cut(s) 22, 171, 255, 647
FokI GGATG 1 cut(s) 337
Fsp4HI GCNGC 4 cut(s) 22, 171, 255, 647
FspBI CTAG 1 cut(s) 245
GlaI GCGC 1 cut(s) 297
GluI GCNGC 4 cut(s) 22, 171, 255, 647
GsaI CCCAGC 1 cut(s) 393
GsuI CTGGAG 1 cut(s) 71
HaeIII GGCC 2 cut(s) 270, 285
HhaI GCGC 1 cut(s) 298
Hin1II CATG 2 cut(s) 150, 325
Hin6I GCGC 1 cut(s) 296
HinP1I GCGC 1 cut(s) 296
HinfI GANTC 4 cut(s) 74, 154, 241, 532
HphI GGTGA 1 cut(s) 337
Hpy188I TCNGA 6 cut(s) 79, 355, 483, 574, 626, 656
Hpy188III TCNNGA 1 cut(s) 578
HpyAV CCTTC 1 cut(s) 431
HpyCH4III ACNGT 3 cut(s) 360, 508, 593
HpyCH4IV ACGT 1 cut(s) 104
HpyCH4V TGCA 6 cut(s) 24, 54, 71, 150, 257, 434
HpyF10VI GCNNNNNNNGC 3 cut(s) 118, 147, 327
HpyF3I CTNAG 3 cut(s) 76, 235, 514
HpySE526I ACGT 1 cut(s) 104
Hsp92II CATG 2 cut(s) 150, 325
HspAI GCGC 1 cut(s) 296
Kzo9I GATC 1 cut(s) 574
LmnI GCTCC 1 cut(s) 236
Lsp1109I GCAGC 4 cut(s) 8, 157, 241, 658
LweI GCATC 2 cut(s) 174, 359
MaeI CTAG 1 cut(s) 245
MaeII ACGT 1 cut(s) 104
MaeIII GTNAC 2 cut(s) 238, 247
MalI GATC 1 cut(s) 576
MboI GATC 1 cut(s) 574
MboII GAAGA 1 cut(s) 121
MfeI CAATTG 1 cut(s) 291
MluCI AATT 6 cut(s) 220, 291, 378, 484, 549, 650
MlyI GAGTC 2 cut(s) 68, 235
MmeI TCCRAC 1 cut(s) 445
MnlI CCTC 7 cut(s) 95, 199, 244, 281, 296, 341, 354
MseI TTAA 2 cut(s) 639, 662
MspR9I CCNGG 5 cut(s) 89, 114, 207, 267, 537
MunI CAATTG 1 cut(s) 291
MvaI CCWGG 5 cut(s) 89, 114, 207, 267, 537
MvnI CGCG 1 cut(s) 298
MwoI GCNNNNNNNGC 3 cut(s) 118, 147, 327
NdeI CATATG 1 cut(s) 674
NdeII GATC 1 cut(s) 574
NlaIII CATG 2 cut(s) 150, 325
NlaIV GGNNCC 2 cut(s) 232, 559
NmuCI GTSAC 2 cut(s) 238, 247
PceI AGGCCT 1 cut(s) 270
PcsI WCGNNNNNNNCGW 1 cut(s) 174
PfeI GAWTC 2 cut(s) 154, 532
PfoI TCCNGGA 1 cut(s) 87
PkrI GCNGC 4 cut(s) 23, 172, 256, 648
PleI GAGTC 2 cut(s) 68, 235
PpsI GAGTC 2 cut(s) 68, 235
PshAI GACNNNNGTC 1 cut(s) 245
Psp6I CCWGG 5 cut(s) 87, 112, 205, 265, 535
PspFI CCCAGC 1 cut(s) 389
PspGI CCWGG 5 cut(s) 87, 112, 205, 265, 535
PspN4I GGNNCC 2 cut(s) 232, 559
PstI CTGCAG 2 cut(s) 73, 259
RsaI GTAC 2 cut(s) 384, 494
RsaNI GTAC 2 cut(s) 383, 493
SaqAI TTAA 2 cut(s) 639, 662
SatI GCNGC 4 cut(s) 22, 171, 255, 647
Sau3AI GATC 1 cut(s) 574
SchI GAGTC 2 cut(s) 68, 235
ScrFI CCNGG 5 cut(s) 89, 114, 207, 267, 537
SetI ASST 6 cut(s) 87, 107, 191, 198, 208, 429
SfaNI GCATC 2 cut(s) 174, 359
SfcI CTRYAG 2 cut(s) 69, 255
SmlI CTYRAG 1 cut(s) 307
SmoI CTYRAG 1 cut(s) 307
Sse9I AATT 6 cut(s) 220, 291, 378, 484, 549, 650
SseBI AGGCCT 1 cut(s) 270
SsiI CCGC 1 cut(s) 110
SspMI CTAG 1 cut(s) 245
StuI AGGCCT 1 cut(s) 270
StyD4I CCNGG 5 cut(s) 87, 112, 205, 265, 535
TaaI ACNGT 3 cut(s) 360, 508, 593
TaiI ACGT 1 cut(s) 107
TasI AATT 6 cut(s) 220, 291, 378, 484, 549, 650
TatI WGTACW 1 cut(s) 382
TfiI GAWTC 2 cut(s) 154, 532
Tru1I TTAA 2 cut(s) 639, 662
Tru9I TTAA 2 cut(s) 639, 662
TscAI CASTG 3 cut(s) 243, 256, 511
TseFI GTSAC 2 cut(s) 238, 247
TseI GCWGC 4 cut(s) 21, 170, 254, 646
Tsp45I GTSAC 2 cut(s) 238, 247
TspDTI ATGAA 2 cut(s) 121, 461
TspRI CASTG 3 cut(s) 243, 256, 511
XapI RAATTY 2 cut(s) 484, 549
XspI CTAG 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.