Rorug06G0489600

Germin-like protein subfamily 3 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0016129
Physical Location & Seq
Reverse (-)
60774505 .. 60778663
4159 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0489600.1

Sequence Viewer

Length: 2097 bp
ATGGCGCCGTCTTGGTGGTCGAAAGAGGACAGCCACCGAGGGACGCCGGTGGTGGTGAAGATGGAGAACCCGAACTGGTCCATGGTGGAGCTGGAGGGTCCGACCGACGAGGACTTCCTAATGGCGGATTCCCCGAGCCGAGCCCGAGGCAAAAACGCCAAGCAGTTCACTTGGGTCCTCCTCCTCAAGGCCCACCGCGCCGCCGGCTGCCTCACCTCCCTGGGCTCGGCAATGTTGGGCCTGGCCTCCGCCATACGACGCCGCGTCGCATCCGGAAGGACAGACACAGACACAGACAGCGAGCCACGCGGCGGCGGCGGCGGCAGAGGGCACGAGAACCCGGCGGTGAAGTCGAGATTCTATTTCTGCATCAAGCTGTTTCTGCTGCTCTCTCTGGTTCTGCTGGGGTTTGAGGTCGCCGCGTATTTTAAAGGGTGGCACTTTGGTGGAGCTCCACACCTGCAGCTTCAGTACTTGTGGGAGATGGAGTTTGATGGGGTTAAGGGGGCTTTCGATTGGGTCTATTCCAAATGGGTTTTGATTCGGGTCGGGTATCTCGCTCCGCCGCTTCAGTTTTTGGCCAATGCCTGTATTGTGCTCTTCATGGTTCAGAGCTTGGATAGGTTGATTCTGTGCCTGGGGTGTTTCTGGATCCGGTTCAAGAAGATTAAGCCGGTTCCGAAAGGCGGTCCTTTGGATCTTGAGAATGGCTACTTCCCTATGGTGCTAGTTCAGATCCCCATGTGCAATGAGAAAGAGGTTTATCAGCAGTCAATTGGTGCTGTGTGCAATCTAGACTGGCCCAAGTCGAGGTTGTTGGTTCAAGTTCTTGACGATTCGGATGACCCGACTACGCAGTTTATGATCAAAGAGGAGGTCCAGAAATGGCACAAGGAAGGGGCCAACATTTTGTATCGGCACAGAGTGATTAGAGATGGATACAAGGCTGGCAATCTCAAGTCTGCAATGAATTGTAGCTATGTCAAAGACTACGAGTTTGTTGCCATTTTCGACGCTGATTTTCAGCCCGCTCCTGATTTCCTCAAGAGAACAGTCCCACACTTCCAGGACAATGAAGAACTGGCGCTGGTCCAAGCTAGGTGGTCCTTTGTGAACAGGGATGAGAACCTACTCACAAGGTTGCAGAACATTAATTTGACATTCCACTTTGAAGTCGAACAACAAGTAAATAGTGTCTTCATTAATTTCTTTGGGTTCAACGGGACAGCTGGTGTGTGGAGGATAAAGGCTCTCGAGGACTCTGGTGGGTGGTTGGAGAGGACCACTGTTGAGGACATGGATATTGCTGTCCGTGCTCATCTTCATGGCTGGAAATTCATCTTCCTCAATGATGTTGAGTGCCAGTGCGAAGTACCTGAATCTTACGAAGCTTACCGGAAACAACAACACAGATGGCACTCGGGACCTATGCAGTTGTTTCGCCTATGTTTACCCGATATCATCAAATCCAAGATCAGCATTTGGAAGAAATTCAATTTGATTTTCCTCTTCTTTCTGCTCAGAAAGTTGATATTGCCCTTCTATTCTTTCACCCTATTTTGCATAATTCTTCCCATGACAATGTTCATCCCCGAAGCTGAGCTCCCAGCATGGGTTGTGTGCTACATCCCTGCAACCATGTCATTCCTCAATATCCTCCCTGCCCCAAAAGCCTTCCCTTTCATTGTTCCTTACCTTCTCTTTGAAAACACCATGTCAGTGACCAAGTTCAATGCTATGATATCCGGCCTATTCCAGCTCGGGAGTGCATACGAGTGGGTGGTTACCAAGAAGTCTGGGCGTTCCTCAGAGGGTGATCTTGTCTCCTTAGTCGAGAAAGAGCCAAAGCATCAGAGGGGGGTCTCGGTTCCAGACCTTGCCGAAATGAAGGAAGAAATTCGACAGCAGGAGCAAAAGGCCAAGAAGAAGAAGCATAACAGAATATACACAAAGGAGCTGGCATTGGCTTTCCTTCTTCTAACGGCTTCAGCTCGGAGCCTGCTGTCTGCTCAGGGCATCCATTTCTATTTCTTGTTGTTTCAGGGTATTTCATTCCTGCTGGTTGGTTTAGACTTGATTGGTGAGCAGGTTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

698

Amino Acids

79.94

Weight (kDa)

8.26

Isoelectric Point (pI)

38.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_tranf_2_3 PF13641 240 - 473 1.9e-15 Glycosyltransferase like family 2
Glycos_transf_2 PF00535 245 - 382 6.6e-12 Glycosyl transferase family 2
Glyco_transf_21 PF13506 329 - 473 1.7e-07 Glycosyl transferase family 21
Glyco_trans_2_3 PF13632 334 - 540 5.2e-22 Glycosyl transferase family group 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016129)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G61750
fragaria_vesca FvH4_5g09990
malus_domestica MD14G1144300.v1.1
prunus_persica Prupe.5G138500_v2.0.a1
pyrus_communis pycom14g11990
rosa_chinensis RchiOBHm_Chr7g0188271
rosa_laevigata RLG00000004712
rosa_multiflora Rmu_sc0008750.1_g000009
rosa_roxburghii Rroxscaffold_3G00266540
rosa_rugosa Rorug06G0489600
rosa_samantha Rh7AG094000 Rh7BG095400 Rh7CG095800 Rh7DG096000
rosa_wichuraiana Rw7G008100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 468
Acc36I ACCTGC 2 cut(s) 468, 2077
AccB1I GGYRCC 1 cut(s) 4
AccBSI CCGCTC 1 cut(s) 1031
AccII CGCG 4 cut(s) 198, 264, 309, 422
AccIII TCCGGA 1 cut(s) 272
AclWI GGATC 4 cut(s) 646, 659, 705, 730
AcoI YGGCCR 1 cut(s) 579
AcsI RAATTY 3 cut(s) 1334, 1490, 1896
AcuI CTGAAG 3 cut(s) 452, 554, 1971
AcyI GRCGYC 3 cut(s) 5, 44, 259
AdeI CACNNNGTG 1 cut(s) 925
AfaI GTAC 2 cut(s) 473, 1374
AfiI CCNNNNNNNGG 7 cut(s) 124, 187, 226, 311, 686, 810, 1612
AgsI TTSAA 7 cut(s) 661, 824, 1172, 1219, 1495, 1706, 1732
AhdI GACNNNNNGTC 1 cut(s) 263
AjnI CCWGG 4 cut(s) 219, 240, 636, 1065
AleI CACNNNNGTG 1 cut(s) 444
Alw21I GWGCWC 4 cut(s) 454, 600, 1318, 1605
Alw26I GTCTC 2 cut(s) 1828, 1867
AlwI GGATC 4 cut(s) 646, 659, 705, 730
Ama87I CYCGRG 5 cut(s) 133, 144, 1253, 1420, 1760
Aor13HI TCCGGA 1 cut(s) 272
AoxI GGCC 8 cut(s) 189, 238, 243, 579, 800, 900, 1747, 1917
ApeKI GCWGC 3 cut(s) 207, 385, 463
ApoI RAATTY 3 cut(s) 1334, 1490, 1896
ArsI GACNNNNNNTTYG 4 cut(s) 861, 893, 980, 1012
AseI ATTAAT 2 cut(s) 1152, 1203
Asp700I GAANNNNTTC 2 cut(s) 1490, 1896
AspLEI GCGC 3 cut(s) 7, 200, 1087
AsuC2I CCSGG 1 cut(s) 341
AsuHPI GGTGA 6 cut(s) 67, 205, 358, 1543, 1826, 2093
AvaI CYCGRG 5 cut(s) 133, 144, 1253, 1420, 1760
AvaII GGWCC 9 cut(s) 78, 98, 175, 689, 877, 1090, 1104, 1281, 1424
BaeGI GKGCMC 1 cut(s) 333
BalI TGGCCA 1 cut(s) 581
BamHI GGATCC 1 cut(s) 651
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 4 cut(s) 145, 227, 454, 1605
BauI CACGAG 1 cut(s) 332
BbsI GAAGAC 1 cut(s) 1189
Bbv12I GWGCWC 4 cut(s) 454, 600, 1318, 1605
BbvI GCAGC 3 cut(s) 194, 372, 475
BccI CCATC 5 cut(s) 55, 478, 488, 929, 1407
BceAI ACGGC 1 cut(s) 1998
BcgI CGANNNNNNTGC 4 cut(s) 983, 1017, 1421, 1455
BciT130I CCWGG 4 cut(s) 221, 242, 638, 1067
BciVI GTATCC 1 cut(s) 932
BclI TGATCA 1 cut(s) 864
BcnI CCSGG 1 cut(s) 341
BcoDI GTCTC 2 cut(s) 1828, 1867
BfaI CTAG 3 cut(s) 728, 794, 1098
BfmI CTRYAG 1 cut(s) 461
BfoI RGCGCY 2 cut(s) 8, 1088
BfuAI ACCTGC 2 cut(s) 468, 2077
BfuI GTATCC 1 cut(s) 932
BlpI GCTNAGC 1 cut(s) 1599
BmcAI AGTACT 1 cut(s) 473
Bme1390I CCNGG 5 cut(s) 221, 242, 341, 638, 1067
Bme18I GGWCC 9 cut(s) 78, 98, 175, 689, 877, 1090, 1104, 1281, 1424
BmeRI GACNNNNNGTC 1 cut(s) 263
BmeT110I CYCGRG 5 cut(s) 133, 144, 1253, 1420, 1760
BmiI GGNNCC 9 cut(s) 6, 99, 176, 653, 678, 901, 1425, 1869, 1997
BmrFI CCNGG 5 cut(s) 221, 242, 341, 638, 1067
BmsI GCATC 4 cut(s) 278, 378, 1858, 2025
BpiI GAAGAC 1 cut(s) 1189
BpmI CTGGAG 1 cut(s) 113
Bpu10I CCTNAGC 1 cut(s) 2010
Bpu1102I GCTNAGC 1 cut(s) 1599
BpuEI CTTGAG 4 cut(s) 170, 722, 941, 1028
BpuMI CCSGG 1 cut(s) 341
BsaHI GRCGYC 3 cut(s) 5, 44, 259
BsaI GGTCTC 1 cut(s) 1867
BsaJI CCNNGG 6 cut(s) 37, 81, 145, 219, 220, 637
BsaWI WCCGGW 3 cut(s) 272, 654, 1395
BsaXI ACNNNNNCTCC 2 cut(s) 473, 503
Bsc4I CCNNNNNNNGG 7 cut(s) 124, 187, 226, 311, 686, 810, 1612
Bse118I RCCGGY 3 cut(s) 46, 203, 673
Bse1I ACTGG 4 cut(s) 80, 803, 1086, 1363
Bse3DI GCAATG 3 cut(s) 237, 754, 972
BseAI TCCGGA 1 cut(s) 272
BseBI CCWGG 4 cut(s) 221, 242, 638, 1067
BseDI CCNNGG 6 cut(s) 37, 81, 145, 219, 220, 637
BseGI GGATG 6 cut(s) 269, 847, 1126, 1587, 1626, 2016
BseLI CCNNNNNNNGG 7 cut(s) 124, 187, 226, 311, 686, 810, 1612
BseMI GCAATG 3 cut(s) 237, 754, 972
BseMII CTCAG 4 cut(s) 1534, 1590, 1821, 2024
BseNI ACTGG 4 cut(s) 80, 803, 1086, 1363
BseRI GAGGAG 3 cut(s) 170, 173, 887
BseSI GKGCMC 1 cut(s) 333
BseXI GCAGC 3 cut(s) 194, 372, 475
BseYI CCCAGC 2 cut(s) 403, 1606
Bsh1236I CGCG 4 cut(s) 198, 264, 309, 422
Bsh1285I CGRYCG 1 cut(s) 105
BshFI GGCC 8 cut(s) 191, 240, 245, 581, 802, 902, 1749, 1919
BshNI GGYRCC 1 cut(s) 4
BsiEI CGRYCG 1 cut(s) 105
BsiHKAI GWGCWC 4 cut(s) 454, 600, 1318, 1605
BsiHKCI CYCGRG 5 cut(s) 133, 144, 1253, 1420, 1760
BsiSI CCGG 8 cut(s) 47, 204, 273, 341, 655, 674, 1396, 1746
BslFI GGGAC 4 cut(s) 55, 1040, 1237, 1437
BslI CCNNNNNNNGG 7 cut(s) 124, 187, 226, 311, 686, 810, 1612
BsmAI GTCTC 2 cut(s) 1828, 1867
BsmFI GGGAC 4 cut(s) 55, 1040, 1237, 1437
BsnI GGCC 8 cut(s) 191, 240, 245, 581, 802, 902, 1749, 1919
Bso31I GGTCTC 1 cut(s) 1867
BsoBI CYCGRG 5 cut(s) 133, 144, 1253, 1420, 1760
Bsp1286I GDGCHC 7 cut(s) 145, 227, 333, 454, 600, 1318, 1605
Bsp13I TCCGGA 1 cut(s) 272
Bsp143I GATC 6 cut(s) 651, 697, 735, 864, 1473, 1816
Bsp1720I GCTNAGC 1 cut(s) 1599
Bsp19I CCATGG 1 cut(s) 81
BspANI GGCC 8 cut(s) 191, 240, 245, 581, 802, 902, 1749, 1919
BspCNI CTCAG 4 cut(s) 1533, 1591, 1820, 2023
BspEI TCCGGA 1 cut(s) 272
BspFNI CGCG 4 cut(s) 198, 264, 309, 422
BspLI GGNNCC 9 cut(s) 6, 99, 176, 653, 678, 901, 1425, 1869, 1997
BspMAI CTGCAG 1 cut(s) 465
BspMI ACCTGC 2 cut(s) 468, 2077
BspPI GGATC 4 cut(s) 646, 659, 705, 730
BspQI GCTCTTC 1 cut(s) 605
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 1 cut(s) 1867
BsrBI CCGCTC 1 cut(s) 1031
BsrDI GCAATG 3 cut(s) 237, 754, 972
BsrFI RCCGGY 3 cut(s) 46, 203, 673
BsrI ACTGG 4 cut(s) 80, 803, 1086, 1363
BssAI RCCGGY 3 cut(s) 46, 203, 673
BssECI CCNNGG 6 cut(s) 37, 81, 145, 219, 220, 637
BssMI GATC 6 cut(s) 651, 697, 735, 864, 1473, 1816
BssNI GRCGYC 3 cut(s) 5, 44, 259
BssSI CACGAG 1 cut(s) 332
BssT1I CCWWGG 1 cut(s) 81
Bst2BI CACGAG 1 cut(s) 332
Bst2UI CCWGG 4 cut(s) 221, 242, 638, 1067
Bst4CI ACNGT 2 cut(s) 1054, 1288
Bst6I CTCTTC 2 cut(s) 605, 1514
BstACI GRCGYC 3 cut(s) 5, 44, 259
BstC8I GCNNGC 6 cut(s) 205, 302, 949, 1029, 1959, 2000
BstDEI CTNAG 5 cut(s) 1520, 1599, 1807, 1828, 2010
BstDSI CCRYGG 1 cut(s) 81
BstEII GGTNACC 1 cut(s) 1783
BstENI CCTNNNNNAGG 1 cut(s) 185
BstF5I GGATG 6 cut(s) 269, 847, 1126, 1587, 1626, 2016
BstFNI CGCG 4 cut(s) 198, 264, 309, 422
BstH2I RGCGCY 2 cut(s) 8, 1088
BstHHI GCGC 3 cut(s) 7, 200, 1087
BstKTI GATC 6 cut(s) 654, 700, 738, 867, 1476, 1819
BstMAI GTCTC 2 cut(s) 1828, 1867
BstMBI GATC 6 cut(s) 651, 697, 735, 864, 1473, 1816
BstMCI CGRYCG 1 cut(s) 105
BstMWI GCNNNNNNNGC 9 cut(s) 197, 204, 306, 315, 318, 321, 382, 1313, 1670
BstNI CCWGG 4 cut(s) 221, 242, 638, 1067
BstPI GGTNACC 1 cut(s) 1783
BstSCI CCNGG 5 cut(s) 219, 240, 339, 636, 1065
BstSFI CTRYAG 1 cut(s) 461
BstSLI GKGCMC 1 cut(s) 333
BstUI CGCG 4 cut(s) 198, 264, 309, 422
BstV1I GCAGC 3 cut(s) 194, 372, 475
BstV2I GAAGAC 1 cut(s) 1189
BstX2I RGATCY 3 cut(s) 651, 697, 735
BstYI RGATCY 3 cut(s) 651, 697, 735
BsuI GTATCC 1 cut(s) 932
BsuRI GGCC 8 cut(s) 191, 240, 245, 581, 802, 902, 1749, 1919
BtgI CCRYGG 1 cut(s) 81
BtsCI GGATG 6 cut(s) 269, 847, 1126, 1587, 1626, 2016
BtsIMutI CAGTG 3 cut(s) 1284, 1370, 1725
BveI ACCTGC 2 cut(s) 468, 2077
Cac8I GCNNGC 6 cut(s) 205, 302, 949, 1029, 1959, 2000
CfoI GCGC 3 cut(s) 7, 200, 1087
Cfr10I RCCGGY 3 cut(s) 46, 203, 673
CseI GACGC 4 cut(s) 52, 253, 267, 1022
Csp6I GTAC 2 cut(s) 472, 1373
CspCI CAANNNNNGTGG 2 cut(s) 1082, 1117
CviAII CATG 9 cut(s) 82, 604, 742, 1297, 1325, 1576, 1611, 1639, 1714
CviQI GTAC 2 cut(s) 472, 1373
DdeI CTNAG 5 cut(s) 1520, 1599, 1807, 1828, 2010
DinI GGCGCC 1 cut(s) 6
DpnI GATC 6 cut(s) 653, 699, 737, 866, 1475, 1818
DpnII GATC 6 cut(s) 651, 697, 735, 864, 1473, 1816
DraI TTTAAA 1 cut(s) 430
DraIII CACNNNGTG 1 cut(s) 925
DriI GACNNNNNGTC 1 cut(s) 263
EaeI YGGCCR 1 cut(s) 579
Eam1104I CTCTTC 2 cut(s) 605, 1514
Eam1105I GACNNNNNGTC 1 cut(s) 263
EarI CTCTTC 2 cut(s) 605, 1514
EciI GGCGGA 3 cut(s) 140, 238, 552
Ecl136II GAGCTC 2 cut(s) 452, 1603
Eco130I CCWWGG 1 cut(s) 81
Eco24I GRGCYC 4 cut(s) 145, 227, 454, 1605
Eco31I GGTCTC 1 cut(s) 1867
Eco32I GATATC 2 cut(s) 1459, 1743
Eco47I GGWCC 9 cut(s) 78, 98, 175, 689, 877, 1090, 1104, 1281, 1424
Eco53kI GAGCTC 2 cut(s) 452, 1603
Eco57I CTGAAG 3 cut(s) 452, 554, 1971
Eco88I CYCGRG 5 cut(s) 133, 144, 1253, 1420, 1760
Eco91I GGTNACC 1 cut(s) 1783
EcoICRI GAGCTC 2 cut(s) 452, 1603
EcoNI CCTNNNNNAGG 1 cut(s) 185
EcoO109I RGGNCCY 2 cut(s) 175, 1424
EcoO65I GGTNACC 1 cut(s) 1783
EcoRII CCWGG 4 cut(s) 219, 240, 636, 1065
EcoRV GATATC 2 cut(s) 1459, 1743
EcoT14I CCWWGG 1 cut(s) 81
EcoT38I GRGCYC 4 cut(s) 145, 227, 454, 1605
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 81
FaeI CATG 9 cut(s) 85, 607, 745, 1300, 1328, 1579, 1614, 1642, 1717
FaqI GGGAC 4 cut(s) 55, 1040, 1237, 1437
FatI CATG 9 cut(s) 81, 603, 741, 1296, 1324, 1575, 1610, 1638, 1713
FauI CCCGC 1 cut(s) 1036
FbaI TGATCA 1 cut(s) 864
FokI GGATG 6 cut(s) 256, 854, 1133, 1574, 1613, 2003
FriOI GRGCYC 4 cut(s) 145, 227, 454, 1605
FspBI CTAG 3 cut(s) 728, 794, 1098
GlaI GCGC 3 cut(s) 6, 199, 1086
GsaI CCCAGC 2 cut(s) 407, 1610
GsuI CTGGAG 1 cut(s) 113
HaeII RGCGCY 2 cut(s) 8, 1088
HaeIII GGCC 8 cut(s) 191, 240, 245, 581, 802, 902, 1749, 1919
HapII CCGG 8 cut(s) 47, 204, 273, 341, 655, 674, 1396, 1746
HgaI GACGC 4 cut(s) 52, 253, 267, 1022
HhaI GCGC 3 cut(s) 7, 200, 1087
Hin1I GRCGYC 3 cut(s) 5, 44, 259
Hin1II CATG 9 cut(s) 85, 607, 745, 1300, 1328, 1579, 1614, 1642, 1717
Hin6I GCGC 3 cut(s) 5, 198, 1085
HinP1I GCGC 3 cut(s) 5, 198, 1085
HindIII AAGCTT 1 cut(s) 1389
HinfI GANTC 7 cut(s) 128, 357, 541, 628, 836, 1259, 1379
HpaII CCGG 8 cut(s) 47, 204, 273, 341, 655, 674, 1396, 1746
HphI GGTGA 6 cut(s) 67, 205, 358, 1543, 1826, 2093
Hpy166II GTNNAC 3 cut(s) 168, 1114, 1451
Hpy188I TCNGA 9 cut(s) 102, 612, 681, 735, 841, 1523, 1810, 1854, 1995
Hpy8I GTNNAC 3 cut(s) 168, 1114, 1451
Hpy99I CGWCG 4 cut(s) 110, 261, 269, 1016
HpyAV CCTTC 7 cut(s) 270, 890, 1549, 1684, 1706, 1882, 1982
HpyCH4III ACNGT 2 cut(s) 1054, 1288
HpyF10VI GCNNNNNNNGC 9 cut(s) 197, 204, 306, 315, 318, 321, 382, 1313, 1670
HpyF3I CTNAG 5 cut(s) 1520, 1599, 1807, 1828, 2010
Hsp92I GRCGYC 3 cut(s) 5, 44, 259
Hsp92II CATG 9 cut(s) 85, 607, 745, 1300, 1328, 1579, 1614, 1642, 1717
HspAI GCGC 3 cut(s) 5, 198, 1085
KasI GGCGCC 1 cut(s) 4
Kpn2I TCCGGA 1 cut(s) 272
KroI GCCGGC 1 cut(s) 203
KroNI GCCGGC 1 cut(s) 205
Ksp22I TGATCA 1 cut(s) 864
Kzo9I GATC 6 cut(s) 651, 697, 735, 864, 1473, 1816
LguI GCTCTTC 1 cut(s) 605
LmnI GCTCC 9 cut(s) 88, 449, 457, 565, 1036, 1608, 1909, 1954, 1995
Lsp1109I GCAGC 3 cut(s) 194, 372, 475
LweI GCATC 4 cut(s) 278, 378, 1858, 2025
MaeI CTAG 3 cut(s) 728, 794, 1098
MaeIII GTNAC 2 cut(s) 1720, 1783
MalI GATC 6 cut(s) 653, 699, 737, 866, 1475, 1818
MbiI CCGCTC 1 cut(s) 1031
MboI GATC 6 cut(s) 651, 697, 735, 864, 1473, 1816
MfeI CAATTG 1 cut(s) 774
MflI RGATCY 3 cut(s) 651, 697, 735
MhlI GDGCHC 7 cut(s) 145, 227, 333, 454, 600, 1318, 1605
MlsI TGGCCA 1 cut(s) 581
MluCI AATT 9 cut(s) 774, 970, 1153, 1204, 1334, 1490, 1495, 1566, 1896
MluNI TGGCCA 1 cut(s) 581
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 1253
MmeI TCCRAC 2 cut(s) 125, 1254
Mox20I TGGCCA 1 cut(s) 581
MroI TCCGGA 1 cut(s) 272
MroNI GCCGGC 1 cut(s) 203
MroXI GAANNNNTTC 2 cut(s) 1490, 1896
MscI TGGCCA 1 cut(s) 581
MseI TTAA 5 cut(s) 429, 501, 669, 1152, 1203
MslI CAYNNNNRTG 5 cut(s) 444, 1323, 1580, 1718, 1774
Msp20I TGGCCA 1 cut(s) 581
MspA1I CMGCKG 1 cut(s) 1229
MspI CCGG 8 cut(s) 47, 204, 273, 341, 655, 674, 1396, 1746
MspR9I CCNGG 5 cut(s) 221, 242, 341, 638, 1067
MunI CAATTG 1 cut(s) 774
MvaI CCWGG 4 cut(s) 221, 242, 638, 1067
MvnI CGCG 4 cut(s) 198, 264, 309, 422
MwoI GCNNNNNNNGC 9 cut(s) 197, 204, 306, 315, 318, 321, 382, 1313, 1670
NaeI GCCGGC 1 cut(s) 205
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 1 cut(s) 341
NcoI CCATGG 1 cut(s) 81
NdeII GATC 6 cut(s) 651, 697, 735, 864, 1473, 1816
NgoMIV GCCGGC 1 cut(s) 203
NlaIII CATG 9 cut(s) 85, 607, 745, 1300, 1328, 1579, 1614, 1642, 1717
NlaIV GGNNCC 9 cut(s) 6, 99, 176, 653, 678, 901, 1425, 1869, 1997
NmeAIII GCCGAG 2 cut(s) 164, 206
NmuCI GTSAC 1 cut(s) 1720
OliI CACNNNNGTG 1 cut(s) 444
PaeR7I CTCGAG 1 cut(s) 1253
PaqCI CACCTGC 1 cut(s) 468
PasI CCCWGGG 1 cut(s) 220
PciSI GCTCTTC 1 cut(s) 605
PcsI WCGNNNNNNNCGW 1 cut(s) 845
PdiI GCCGGC 1 cut(s) 205
PdmI GAANNNNTTC 2 cut(s) 1490, 1896
PfeI GAWTC 6 cut(s) 128, 357, 541, 628, 836, 1379
PfoI TCCNGGA 1 cut(s) 1065
PleI GAGTC 1 cut(s) 1253
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 1253
PpuMI RGGWCCY 2 cut(s) 175, 1424
PshBI ATTAAT 2 cut(s) 1152, 1203
Psp124BI GAGCTC 2 cut(s) 454, 1605
Psp5II RGGWCCY 2 cut(s) 175, 1424
Psp6I CCWGG 4 cut(s) 219, 240, 636, 1065
PspEI GGTNACC 1 cut(s) 1783
PspFI CCCAGC 2 cut(s) 403, 1606
PspGI CCWGG 4 cut(s) 219, 240, 636, 1065
PspN4I GGNNCC 9 cut(s) 6, 99, 176, 653, 678, 901, 1425, 1869, 1997
PspPPI RGGWCCY 2 cut(s) 175, 1424
PstI CTGCAG 1 cut(s) 465
PsuI RGATCY 3 cut(s) 651, 697, 735
PvuII CAGCTG 1 cut(s) 1229
RsaI GTAC 2 cut(s) 473, 1374
RsaNI GTAC 2 cut(s) 472, 1373
RseI CAYNNNNRTG 5 cut(s) 444, 1323, 1580, 1718, 1774
SacI GAGCTC 2 cut(s) 454, 1605
SapI GCTCTTC 1 cut(s) 605
SaqAI TTAA 5 cut(s) 429, 501, 669, 1152, 1203
Sau3AI GATC 6 cut(s) 651, 697, 735, 864, 1473, 1816
ScaI AGTACT 1 cut(s) 473
SchI GAGTC 1 cut(s) 1253
ScrFI CCNGG 5 cut(s) 221, 242, 341, 638, 1067
SduI GDGCHC 7 cut(s) 145, 227, 333, 454, 600, 1318, 1605
SfaNI GCATC 4 cut(s) 278, 378, 1858, 2025
SfcI CTRYAG 1 cut(s) 461
SfoI GGCGCC 1 cut(s) 6
Sfr274I CTCGAG 1 cut(s) 1253
SgrAI CRCCGGYG 1 cut(s) 46
SinI GGWCC 9 cut(s) 78, 98, 175, 689, 877, 1090, 1104, 1281, 1424
SlaI CTCGAG 1 cut(s) 1253
SmiMI CAYNNNNRTG 5 cut(s) 444, 1323, 1580, 1718, 1774
SmlI CTYRAG 5 cut(s) 185, 701, 956, 1043, 1253
SmoI CTYRAG 5 cut(s) 185, 701, 956, 1043, 1253
Sse9I AATT 9 cut(s) 774, 970, 1153, 1204, 1334, 1490, 1495, 1566, 1896
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 3 cut(s) 728, 794, 1098
SstI GAGCTC 2 cut(s) 454, 1605
StyD4I CCNGG 5 cut(s) 219, 240, 339, 636, 1065
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 2 cut(s) 1054, 1288
TaqI TCGA 9 cut(s) 20, 353, 513, 809, 1011, 1176, 1254, 1833, 1900
TaqII GACCGA 1 cut(s) 119
TasI AATT 9 cut(s) 774, 970, 1153, 1204, 1334, 1490, 1495, 1566, 1896
TatI WGTACW 1 cut(s) 471
TauI GCSGC 9 cut(s) 203, 264, 312, 315, 318, 321, 324, 422, 568
TfiI GAWTC 6 cut(s) 128, 357, 541, 628, 836, 1379
Tru1I TTAA 5 cut(s) 429, 501, 669, 1152, 1203
Tru9I TTAA 5 cut(s) 429, 501, 669, 1152, 1203
TscAI CASTG 3 cut(s) 1291, 1370, 1725
TseFI GTSAC 1 cut(s) 1720
TseI GCWGC 3 cut(s) 207, 385, 463
Tsp45I GTSAC 1 cut(s) 1720
TspGWI ACGGA 1 cut(s) 1301
TspRI CASTG 3 cut(s) 1291, 1370, 1725
VpaK11BI GGWCC 9 cut(s) 78, 98, 175, 689, 877, 1090, 1104, 1281, 1424
VspI ATTAAT 2 cut(s) 1152, 1203
XagI CCTNNNNNAGG 1 cut(s) 185
XapI RAATTY 3 cut(s) 1334, 1490, 1896
XbaI TCTAGA 1 cut(s) 793
XcmI CCANNNNNNNNNTGG 1 cut(s) 88
XhoI CTCGAG 1 cut(s) 1253
XmnI GAANNNNTTC 2 cut(s) 1490, 1896
XspI CTAG 3 cut(s) 728, 794, 1098
ZrmI AGTACT 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.