Rroxscaffold_3G00266540

Germin-like protein subfamily 3 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0016129
Physical Location & Seq
Forward (+)
59787368 .. 59788042
675 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00266540.1

Sequence Viewer

Length: 675 bp
ATGAATAGTCCATCTTTTGCTTGCTGCTTTGTCTTTCTATCAGTGATCTTCTGTTTCTGCACTAAAATCTCCTTGGCGGATTCCGATAATCTCCAGGACACATGCCCTACATCCCAGCTAGCAAAACAAACCATCTTCATCAATGGCTTCCTTTGCAAGAATCCAAACGACATCAGCTCCCAAGATTTCAAGACTACCAAACTGACCCAACCGGGCGACACAGACAACTTCTCTGGCTCCGCAGTAACCATTGTCACTGCTGCAGAGTTTCCAGGCTTAAACACTCTTGGCCTCTCAATTGCAAGAACTGACTTGAGAGTTGATGGTCTGGTGAGGCTCCACTCCCACCCAAGAGCTACGGAAATGTTCTTTGTCAGCAAAGGCCTCGTGCTAGTGGGGTTCATCGACACATCAAACCAGCTATTTGGGACGTTTCTTAAGGAAGGAGACGTGTTCGTGTTCCCCAGGGGCCTGCTTCACTTTTGTTTGAATCCTGGCTATGGCACTGCCACTGGTTTATCGGTGTATAATAGCCAGAATCCAGGGGTGGTGACCGCTTCTGATGTCATGTTTGAGCCTAAGCCGGATGTGATAAAGAAGCTTACAAAAGGGTTTGTCACTGGTCACCTTAGAAATGCTACTCTAAGGGAATTTCAAGTTTTTAACATGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

224

Amino Acids

24.49

Weight (kDa)

6.88

Isoelectric Point (pI)

17.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 67 - 192 4.6e-32 Cupin
Cupin_2 PF07883 113 - 176 7.5e-06 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016129)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G61750
fragaria_vesca FvH4_5g09990
malus_domestica MD14G1144300.v1.1
prunus_persica Prupe.5G138500_v2.0.a1
pyrus_communis pycom14g11990
rosa_chinensis RchiOBHm_Chr7g0188271
rosa_laevigata RLG00000004712
rosa_multiflora Rmu_sc0008750.1_g000009
rosa_roxburghii Rroxscaffold_3G00266540
rosa_rugosa Rorug06G0489600
rosa_samantha Rh7AG094000 Rh7BG095400 Rh7CG095800 Rh7DG096000
rosa_wichuraiana Rw7G008100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 77, 240, 555
AcsI RAATTY 1 cut(s) 650
AfiI CCNNNNNNNGG 1 cut(s) 500
AflII CTTAAG 1 cut(s) 437
AflIII ACRYGT 1 cut(s) 450
AgsI TTSAA 3 cut(s) 190, 490, 656
AjiI CACGTC 1 cut(s) 451
AjnI CCWGG 5 cut(s) 93, 271, 464, 493, 541
AluBI AGCT 5 cut(s) 118, 177, 356, 421, 601
AluI AGCT 5 cut(s) 118, 177, 356, 421, 601
Alw26I GTCTC 1 cut(s) 441
AoxI GGCC 3 cut(s) 289, 382, 469
ApeKI GCWGC 2 cut(s) 24, 260
ApoI RAATTY 1 cut(s) 650
AspS9I GGNCC 1 cut(s) 469
AsuC2I CCSGG 1 cut(s) 213
AsuHPI GGTGA 3 cut(s) 343, 562, 617
AsuNHI GCTAGC 1 cut(s) 118
BauI CACGAG 1 cut(s) 386
BbvI GCAGC 2 cut(s) 11, 247
BccI CCATC 3 cut(s) 19, 140, 317
BcgI CGANNNNNNTGC 2 cut(s) 367, 401
BciT130I CCWGG 5 cut(s) 95, 273, 466, 495, 543
BcnI CCSGG 1 cut(s) 213
BcoDI GTCTC 1 cut(s) 441
BfaI CTAG 2 cut(s) 119, 392
BfmI CTRYAG 1 cut(s) 261
BfrI CTTAAG 1 cut(s) 437
BisI GCNGC 2 cut(s) 25, 261
BlsI GCNGC 2 cut(s) 26, 262
Bme1390I CCNGG 6 cut(s) 95, 213, 273, 466, 495, 543
BmgBI CACGTC 1 cut(s) 451
BmgT120I GGNCC 1 cut(s) 469
BmiI GGNNCC 3 cut(s) 238, 338, 470
BmrFI CCNGG 6 cut(s) 95, 213, 273, 466, 495, 543
BmtI GCTAGC 1 cut(s) 122
BpmI CTGGAG 1 cut(s) 77
Bpu10I CCTNAGC 1 cut(s) 579
BpuEI CTTGAG 1 cut(s) 334
BpuMI CCSGG 1 cut(s) 213
BsaJI CCNNGG 4 cut(s) 72, 464, 465, 542
BsaXI ACNNNNNCTCC 2 cut(s) 161, 191
Bsc4I CCNNNNNNNGG 1 cut(s) 500
Bse1I ACTGG 2 cut(s) 517, 625
BseBI CCWGG 5 cut(s) 95, 273, 466, 495, 543
BseDI CCNNGG 4 cut(s) 72, 464, 465, 542
BseGI GGATG 2 cut(s) 110, 592
BseLI CCNNNNNNNGG 1 cut(s) 500
BseNI ACTGG 2 cut(s) 517, 625
BseXI GCAGC 2 cut(s) 11, 247
BseYI CCCAGC 1 cut(s) 114
BsgI GTGCAG 1 cut(s) 43
BshFI GGCC 3 cut(s) 291, 384, 471
BsiSI CCGG 2 cut(s) 212, 584
BslFI GGGAC 1 cut(s) 442
BslI CCNNNNNNNGG 1 cut(s) 500
BsmAI GTCTC 1 cut(s) 441
BsmBI CGTCTC 1 cut(s) 441
BsmFI GGGAC 1 cut(s) 442
BsnI GGCC 3 cut(s) 291, 384, 471
Bsp143I GATC 1 cut(s) 45
BspACI CCGC 3 cut(s) 77, 240, 555
BspANI GGCC 3 cut(s) 291, 384, 471
BspLI GGNNCC 3 cut(s) 238, 338, 470
BspMAI CTGCAG 1 cut(s) 265
BspOI GCTAGC 1 cut(s) 122
BspTI CTTAAG 1 cut(s) 437
BsrI ACTGG 2 cut(s) 517, 625
BssECI CCNNGG 4 cut(s) 72, 464, 465, 542
BssMI GATC 1 cut(s) 45
BssSI CACGAG 1 cut(s) 386
BssT1I CCWWGG 1 cut(s) 72
Bst2BI CACGAG 1 cut(s) 386
Bst2UI CCWGG 5 cut(s) 95, 273, 466, 495, 543
BstAFI CTTAAG 1 cut(s) 437
BstC8I GCNNGC 3 cut(s) 22, 120, 473
BstDEI CTNAG 3 cut(s) 579, 629, 644
BstEII GGTNACC 2 cut(s) 550, 623
BstF5I GGATG 2 cut(s) 110, 592
BstKTI GATC 1 cut(s) 48
BstMAI GTCTC 1 cut(s) 441
BstMBI GATC 1 cut(s) 45
BstMWI GCNNNNNNNGC 1 cut(s) 153
BstNI CCWGG 5 cut(s) 95, 273, 466, 495, 543
BstNSI RCATGY 2 cut(s) 105, 670
BstPI GGTNACC 2 cut(s) 550, 623
BstSCI CCNGG 6 cut(s) 93, 211, 271, 464, 493, 541
BstSFI CTRYAG 1 cut(s) 261
BstV1I GCAGC 2 cut(s) 11, 247
BstXI CCANNNNNNTGG 1 cut(s) 425
BsuRI GGCC 3 cut(s) 291, 384, 471
BtrI CACGTC 1 cut(s) 451
BtsCI GGATG 2 cut(s) 110, 592
BtsI GCAGTG 2 cut(s) 255, 504
BtsIMutI CAGTG 5 cut(s) 48, 255, 504, 510, 618
Cac8I GCNNGC 3 cut(s) 22, 120, 473
Cfr13I GGNCC 1 cut(s) 469
CviAII CATG 3 cut(s) 102, 568, 667
DdeI CTNAG 3 cut(s) 579, 629, 644
DpnI GATC 1 cut(s) 47
DpnII GATC 1 cut(s) 45
EciI GGCGGA 1 cut(s) 92
Eco130I CCWWGG 1 cut(s) 72
Eco147I AGGCCT 1 cut(s) 384
Eco91I GGTNACC 2 cut(s) 550, 623
EcoO109I RGGNCCY 1 cut(s) 469
EcoO65I GGTNACC 2 cut(s) 550, 623
EcoRII CCWGG 5 cut(s) 93, 271, 464, 493, 541
EcoT14I CCWWGG 1 cut(s) 72
ErhI CCWWGG 1 cut(s) 72
Esp3I CGTCTC 1 cut(s) 441
FaeI CATG 3 cut(s) 105, 571, 670
FaiI YATR 5 cut(s) 103, 501, 528, 569, 668
FaqI GGGAC 1 cut(s) 442
FatI CATG 3 cut(s) 101, 567, 666
Fnu4HI GCNGC 2 cut(s) 25, 261
FokI GGATG 2 cut(s) 97, 599
Fsp4HI GCNGC 2 cut(s) 25, 261
FspBI CTAG 2 cut(s) 119, 392
GluI GCNGC 2 cut(s) 25, 261
GsaI CCCAGC 1 cut(s) 118
GsuI CTGGAG 1 cut(s) 77
HaeIII GGCC 3 cut(s) 291, 384, 471
HapII CCGG 2 cut(s) 212, 584
Hin1II CATG 3 cut(s) 105, 571, 670
HindIII AAGCTT 1 cut(s) 599
HinfI GANTC 4 cut(s) 80, 160, 490, 538
HpaII CCGG 2 cut(s) 212, 584
HphI GGTGA 3 cut(s) 343, 562, 617
Hpy188I TCNGA 2 cut(s) 85, 562
Hpy188III TCNNGA 1 cut(s) 190
HpyAV CCTTC 1 cut(s) 437
HpyCH4IV ACGT 2 cut(s) 431, 450
HpyCH4V TGCA 5 cut(s) 60, 156, 263, 302, 670
HpyF10VI GCNNNNNNNGC 1 cut(s) 153
HpyF3I CTNAG 3 cut(s) 579, 629, 644
HpySE526I ACGT 2 cut(s) 431, 450
Hsp92II CATG 3 cut(s) 105, 571, 670
Kzo9I GATC 1 cut(s) 45
LmnI GCTCC 3 cut(s) 182, 242, 342
Lsp1109I GCAGC 2 cut(s) 11, 247
MaeI CTAG 2 cut(s) 119, 392
MaeII ACGT 2 cut(s) 431, 450
MaeIII GTNAC 5 cut(s) 244, 253, 550, 616, 623
MalI GATC 1 cut(s) 47
MboI GATC 1 cut(s) 45
MboII GAAGA 2 cut(s) 40, 127
MfeI CAATTG 1 cut(s) 297
MluCI AATT 2 cut(s) 297, 650
MnlI CCTC 3 cut(s) 302, 327, 395
MseI TTAA 3 cut(s) 278, 438, 663
MspCI CTTAAG 1 cut(s) 437
MspI CCGG 2 cut(s) 212, 584
MspR9I CCNGG 6 cut(s) 95, 213, 273, 466, 495, 543
MunI CAATTG 1 cut(s) 297
MvaI CCWGG 5 cut(s) 95, 273, 466, 495, 543
MwoI GCNNNNNNNGC 1 cut(s) 153
NciI CCSGG 1 cut(s) 213
NdeII GATC 1 cut(s) 45
NheI GCTAGC 1 cut(s) 118
NlaIII CATG 3 cut(s) 105, 571, 670
NlaIV GGNNCC 3 cut(s) 238, 338, 470
NmuCI GTSAC 4 cut(s) 253, 550, 616, 623
NspI RCATGY 2 cut(s) 105, 670
PasI CCCWGGG 1 cut(s) 465
PceI AGGCCT 1 cut(s) 384
PfeI GAWTC 4 cut(s) 80, 160, 490, 538
PfoI TCCNGGA 1 cut(s) 93
PkrI GCNGC 2 cut(s) 26, 262
Psp6I CCWGG 5 cut(s) 93, 271, 464, 493, 541
PspEI GGTNACC 2 cut(s) 550, 623
PspFI CCCAGC 1 cut(s) 114
PspGI CCWGG 5 cut(s) 93, 271, 464, 493, 541
PspN4I GGNNCC 3 cut(s) 238, 338, 470
PspPI GGNCC 1 cut(s) 469
PstI CTGCAG 1 cut(s) 265
SaqAI TTAA 3 cut(s) 278, 438, 663
SatI GCNGC 2 cut(s) 25, 261
Sau3AI GATC 1 cut(s) 45
Sau96I GGNCC 1 cut(s) 469
ScrFI CCNGG 6 cut(s) 95, 213, 273, 466, 495, 543
SetI ASST 8 cut(s) 120, 179, 358, 423, 434, 453, 603, 630
SfcI CTRYAG 1 cut(s) 261
SmlI CTYRAG 2 cut(s) 313, 437
SmoI CTYRAG 2 cut(s) 313, 437
Sse9I AATT 2 cut(s) 297, 650
SseBI AGGCCT 1 cut(s) 384
SsiI CCGC 3 cut(s) 77, 240, 555
SspMI CTAG 2 cut(s) 119, 392
StuI AGGCCT 1 cut(s) 384
StyD4I CCNGG 6 cut(s) 93, 211, 271, 464, 493, 541
StyI CCWWGG 1 cut(s) 72
TaiI ACGT 2 cut(s) 434, 453
TaqI TCGA 1 cut(s) 405
TasI AATT 2 cut(s) 297, 650
TfiI GAWTC 4 cut(s) 80, 160, 490, 538
Tru1I TTAA 3 cut(s) 278, 438, 663
Tru9I TTAA 3 cut(s) 278, 438, 663
TscAI CASTG 5 cut(s) 48, 262, 511, 517, 625
TseFI GTSAC 4 cut(s) 253, 550, 616, 623
TseI GCWGC 2 cut(s) 24, 260
Tsp45I GTSAC 4 cut(s) 253, 550, 616, 623
TspDTI ATGAA 3 cut(s) 17, 127, 391
TspGWI ACGGA 1 cut(s) 374
TspRI CASTG 5 cut(s) 48, 262, 511, 517, 625
Vha464I CTTAAG 1 cut(s) 437
XapI RAATTY 1 cut(s) 650
XceI RCATGY 2 cut(s) 105, 670
XspI CTAG 2 cut(s) 119, 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.