MD14G1152800.v1.1

Belongs to the short-chain dehydrogenases reductases (SDR) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0014930
Physical Location & Seq
Forward (+)
24684819 .. 24686687
1869 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1152800.v1.1.491

Sequence Viewer

Length: 1050 bp
ATGGATATTATTAACAAGGTGCTCAACGTTACAATTCCTCCCATGTCCCTCGCCTGGTTGCTCTTCGTTCTGCCAACCTATCTCTTCTGCAAGCTTCTCCTCTCCGCCGCACGACATGTTTTTAGTGAAGATGTGGGCGGAAAAGTCATACTCATTACCGGAGCTTCCTCCGGCATAGGAGAGCATCTGGCATATGAGTATGCTAGAAGAGGAGCTTGTCTAGCTCTTGTTGCCAGAAGAGAAAACCGACTCCGTGACGTTGCCAACACAGCCATTTGCTTTGGTTCCCCAGATGTCCTCGCAATCACTGCAGATGTTTCAAGGGTTGAAGATTGTAAGCGCTTTGTTGATGTAACAGTGAATCATTTTGGACGTTTGGATCACCTAGTGAACAATGCTGGGATTGTTCCTGTCTGCTTGTTTGAAGAAACCACTGATGTCACTAACTTAGCGCCCGCCATGGACATAAACTTTTGGGGCTCAGTATATGGCACCTACTTTGCCATTCCACACCTTAAAAGGAGCGGAGGGAGGATCGTAGCGATCACTTCCTCTGCCGGATGGTTGCGTGCACCACGATTAAGCATGTATGGTGCTACAAAAGCAGCAGCGATAGCCTTTTACGAGACGTTGAAAGTTGAAATTGGGAGGGAAGTTGGAATAACAATCGTGACTCCGGGAATGATTGAATCGGAAATTTCGCAAGGAAAATTCTTATCCAAGGAAGGCCAAGTGGTATTAGATCAAGAATTGAGAGATCTTGAAGTAAGCATAATACCGATCTTGCCGGTGAAGGAGGCCGCCAAATCAATCGTGGACAGCGCATGCCGAGGAGAAAATTATTTGACAGTACCAGCTTGGATTTGGCAGTCATTTTACTGGAAGGTGTTTTTCCCTGAGATACTGGAGTGGTGTAACCGTACGCTTCTTATAAGTAAAGCGGGCAATGACGAGAGGGATACGCTGAGTAAGAAGCTCCTGTACCTATCTGGCCTGAAGCAGCATCTGTATCCAGAGACGGTTCGAGACCCTCATGTTAAAGAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short

Protein Analysis

350

Amino Acids

38.79

Weight (kDa)

6.67

Isoelectric Point (pI)

37.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 48 - 236 5.7e-44 short chain dehydrogenase
KR PF08659 50 - 209 2e-06 KR domain
adh_short_C2 PF13561 54 - 236 5.3e-29 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014930)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50770
fragaria_vesca FvH4_5g08730
malus_domestica MD14G1152800.v1.1
prunus_persica Prupe.5G147800_v2.0.a1
pyrus_communis pycom14g12720 pycom14g12730
rosa_chinensis RchiOBHm_Chr7g0189551
rosa_laevigata RLG00000004601
rosa_multiflora Rmu_sc0001924.1_g000012
rosa_roxburghii Rroxscaffold_3G00265310
rosa_rugosa Rorug06G0498900
rosa_samantha Rh7AG103900 Rh7BG106600 Rh7CG108700 Rh7DG106000
rosa_wichuraiana Rw7G008970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 932
AccB1I GGYRCC 1 cut(s) 491
AccBSI CCGCTC 1 cut(s) 525
AciI CCGC 7 cut(s) 105, 108, 138, 456, 525, 801, 941
AclI AACGTT 1 cut(s) 27
AclWI GGATC 2 cut(s) 387, 542
AcsI RAATTY 2 cut(s) 696, 710
AcuI CTGAAG 1 cut(s) 1016
AdeI CACNNNGTG 1 cut(s) 388
AfaI GTAC 3 cut(s) 852, 922, 983
AfeI AGCGCT 1 cut(s) 341
AfiI CCNNNNNNNGG 1 cut(s) 54
AflIII ACRYGT 1 cut(s) 115
AgsI TTSAA 7 cut(s) 321, 329, 425, 634, 641, 689, 764
AjnI CCWGG 1 cut(s) 53
AluBI AGCT 6 cut(s) 94, 164, 215, 224, 857, 976
AluI AGCT 6 cut(s) 94, 164, 215, 224, 857, 976
Alw21I GWGCWC 2 cut(s) 24, 574
Alw26I GTCTC 3 cut(s) 620, 1010, 1020
Alw44I GTGCAC 1 cut(s) 570
AlwI GGATC 2 cut(s) 387, 542
AlwNI CAGNNNCTG 1 cut(s) 1006
Aor51HI AGCGCT 1 cut(s) 341
AoxI GGCC 3 cut(s) 727, 798, 991
ApaLI GTGCAC 1 cut(s) 570
ApeKI GCWGC 3 cut(s) 605, 608, 1000
ApoI RAATTY 2 cut(s) 696, 710
AspLEI GCGC 3 cut(s) 342, 454, 824
AsuC2I CCSGG 1 cut(s) 678
AsuHPI GGTGA 2 cut(s) 374, 802
BaeGI GKGCMC 1 cut(s) 574
BaeI ACNNNNGTAYC 2 cut(s) 893, 926
BanI GGYRCC 1 cut(s) 491
BanII GRGCYC 1 cut(s) 482
BarI GAAGNNNNNNTAC 2 cut(s) 965, 997
Bbv12I GWGCWC 2 cut(s) 24, 574
BbvI GCAGC 3 cut(s) 617, 620, 1012
BccI CCATC 1 cut(s) 555
BciT130I CCWGG 1 cut(s) 55
BciVI GTATCC 2 cut(s) 952, 1020
BcnI CCSGG 1 cut(s) 678
BcoDI GTCTC 3 cut(s) 620, 1010, 1020
BfaI CTAG 3 cut(s) 204, 221, 386
BfmI CTRYAG 1 cut(s) 309
BfoI RGCGCY 2 cut(s) 343, 455
BfuI GTATCC 2 cut(s) 952, 1020
BglII AGATCT 1 cut(s) 757
BisI GCNGC 5 cut(s) 108, 606, 609, 801, 1001
BlsI GCNGC 5 cut(s) 109, 607, 610, 802, 1002
Bme1390I CCNGG 2 cut(s) 55, 678
BmiI GGNNCC 2 cut(s) 286, 493
BmrFI CCNGG 2 cut(s) 55, 678
BmsI GCATC 2 cut(s) 193, 1012
BpmI CTGGAG 1 cut(s) 926
BpuMI CCSGG 1 cut(s) 678
BsaI GGTCTC 1 cut(s) 1020
BsaJI CCNNGG 3 cut(s) 459, 720, 829
BsaWI WCCGGW 1 cut(s) 158
BsaXI ACNNNNNCTCC 2 cut(s) 22, 52
Bsc4I CCNNNNNNNGG 1 cut(s) 54
Bse118I RCCGGY 1 cut(s) 787
Bse1I ACTGG 2 cut(s) 884, 909
Bse3DI GCAATG 1 cut(s) 952
BseBI CCWGG 1 cut(s) 55
BseDI CCNNGG 3 cut(s) 459, 720, 829
BseGI GGATG 1 cut(s) 566
BseLI CCNNNNNNNGG 1 cut(s) 54
BseMI GCAATG 1 cut(s) 952
BseMII CTCAG 3 cut(s) 495, 888, 956
BseNI ACTGG 2 cut(s) 884, 909
BseRI GAGGAG 3 cut(s) 89, 225, 846
BseSI GKGCMC 1 cut(s) 574
BseXI GCAGC 3 cut(s) 617, 620, 1012
BseYI CCCAGC 1 cut(s) 398
BshFI GGCC 3 cut(s) 729, 800, 993
BshNI GGYRCC 1 cut(s) 491
BsiHKAI GWGCWC 2 cut(s) 24, 574
BsiSI CCGG 5 cut(s) 159, 171, 558, 677, 788
BsiWI CGTACG 1 cut(s) 920
BslFI GGGAC 1 cut(s) 31
BslI CCNNNNNNNGG 1 cut(s) 54
BsmAI GTCTC 3 cut(s) 620, 1010, 1020
BsmBI CGTCTC 2 cut(s) 620, 1010
BsmFI GGGAC 1 cut(s) 31
BsnI GGCC 3 cut(s) 729, 800, 993
Bso31I GGTCTC 1 cut(s) 1020
Bsp1286I GDGCHC 3 cut(s) 24, 482, 574
Bsp143I GATC 6 cut(s) 379, 534, 543, 742, 757, 780
Bsp19I CCATGG 1 cut(s) 459
BspACI CCGC 7 cut(s) 105, 108, 138, 456, 525, 801, 941
BspANI GGCC 3 cut(s) 729, 800, 993
BspCNI CTCAG 3 cut(s) 494, 889, 957
BspLI GGNNCC 2 cut(s) 286, 493
BspMAI CTGCAG 1 cut(s) 313
BspPI GGATC 2 cut(s) 387, 542
BspQI GCTCTTC 1 cut(s) 68
BspT107I GGYRCC 1 cut(s) 491
BspTNI GGTCTC 1 cut(s) 1020
BsrBI CCGCTC 1 cut(s) 525
BsrDI GCAATG 1 cut(s) 952
BsrFI RCCGGY 1 cut(s) 787
BsrI ACTGG 2 cut(s) 884, 909
BssAI RCCGGY 1 cut(s) 787
BssECI CCNNGG 3 cut(s) 459, 720, 829
BssMI GATC 6 cut(s) 379, 534, 543, 742, 757, 780
BssT1I CCWWGG 2 cut(s) 459, 720
Bst2UI CCWGG 1 cut(s) 55
Bst4CI ACNGT 4 cut(s) 358, 850, 920, 1021
Bst6I CTCTTC 4 cut(s) 68, 89, 202, 232
BstAPI GCANNNNNTGC 1 cut(s) 308
BstC8I GCNNGC 5 cut(s) 92, 456, 570, 826, 943
BstDEI CTNAG 4 cut(s) 448, 481, 897, 965
BstDSI CCRYGG 1 cut(s) 459
BstF5I GGATG 1 cut(s) 566
BstH2I RGCGCY 2 cut(s) 343, 455
BstHHI GCGC 3 cut(s) 342, 454, 824
BstKTI GATC 6 cut(s) 382, 537, 546, 745, 760, 783
BstMAI GTCTC 3 cut(s) 620, 1010, 1020
BstMBI GATC 6 cut(s) 379, 534, 543, 742, 757, 780
BstMWI GCNNNNNNNGC 6 cut(s) 221, 230, 269, 308, 602, 614
BstNI CCWGG 1 cut(s) 55
BstNSI RCATGY 3 cut(s) 119, 589, 828
BstSCI CCNGG 2 cut(s) 53, 676
BstSFI CTRYAG 1 cut(s) 309
BstSLI GKGCMC 1 cut(s) 574
BstV1I GCAGC 3 cut(s) 617, 620, 1012
BstX2I RGATCY 1 cut(s) 757
BstYI RGATCY 1 cut(s) 757
BsuI GTATCC 2 cut(s) 952, 1020
BsuRI GGCC 3 cut(s) 729, 800, 993
BtgI CCRYGG 1 cut(s) 459
BtsCI GGATG 1 cut(s) 566
BtsI GCAGTG 1 cut(s) 306
BtsIMutI CAGTG 3 cut(s) 306, 363, 432
Cac8I GCNNGC 5 cut(s) 92, 456, 570, 826, 943
CaiI CAGNNNCTG 1 cut(s) 1006
CfoI GCGC 3 cut(s) 342, 454, 824
Cfr10I RCCGGY 1 cut(s) 787
Csp6I GTAC 3 cut(s) 851, 921, 982
CviAII CATG 6 cut(s) 43, 116, 460, 586, 825, 1034
CviQI GTAC 3 cut(s) 851, 921, 982
DdeI CTNAG 4 cut(s) 448, 481, 897, 965
DpnI GATC 6 cut(s) 381, 536, 545, 744, 759, 782
DpnII GATC 6 cut(s) 379, 534, 543, 742, 757, 780
DraIII CACNNNGTG 1 cut(s) 388
Eam1104I CTCTTC 4 cut(s) 68, 89, 202, 232
EarI CTCTTC 4 cut(s) 68, 89, 202, 232
EciI GGCGGA 2 cut(s) 94, 153
Eco130I CCWWGG 2 cut(s) 459, 720
Eco24I GRGCYC 1 cut(s) 482
Eco31I GGTCTC 1 cut(s) 1020
Eco47III AGCGCT 1 cut(s) 341
Eco57I CTGAAG 1 cut(s) 1016
EcoRII CCWGG 1 cut(s) 53
EcoT14I CCWWGG 2 cut(s) 459, 720
EcoT38I GRGCYC 1 cut(s) 482
ErhI CCWWGG 2 cut(s) 459, 720
Esp3I CGTCTC 2 cut(s) 620, 1010
FaeI CATG 6 cut(s) 46, 119, 463, 589, 828, 1037
FalI AAGNNNNNCTT 2 cut(s) 199, 231
FaqI GGGAC 1 cut(s) 31
FatI CATG 6 cut(s) 42, 115, 459, 585, 824, 1033
FauI CCCGC 2 cut(s) 463, 934
FauNDI CATATG 1 cut(s) 193
Fnu4HI GCNGC 5 cut(s) 108, 606, 609, 801, 1001
FokI GGATG 1 cut(s) 573
FriOI GRGCYC 1 cut(s) 482
Fsp4HI GCNGC 5 cut(s) 108, 606, 609, 801, 1001
FspBI CTAG 3 cut(s) 204, 221, 386
GlaI GCGC 3 cut(s) 341, 453, 823
GluI GCNGC 5 cut(s) 108, 606, 609, 801, 1001
GsaI CCCAGC 1 cut(s) 402
GsuI CTGGAG 1 cut(s) 926
HaeII RGCGCY 2 cut(s) 343, 455
HaeIII GGCC 3 cut(s) 729, 800, 993
HapII CCGG 5 cut(s) 159, 171, 558, 677, 788
HhaI GCGC 3 cut(s) 342, 454, 824
Hin1II CATG 6 cut(s) 46, 119, 463, 589, 828, 1037
Hin6I GCGC 3 cut(s) 340, 452, 822
HinP1I GCGC 3 cut(s) 340, 452, 822
HindIII AAGCTT 1 cut(s) 92
HinfI GANTC 4 cut(s) 249, 361, 673, 689
HpaII CCGG 5 cut(s) 159, 171, 558, 677, 788
HphI GGTGA 2 cut(s) 374, 802
Hpy166II GTNNAC 3 cut(s) 391, 572, 817
Hpy188I TCNGA 1 cut(s) 694
Hpy188III TCNNGA 5 cut(s) 670, 746, 761, 1013, 1025
Hpy8I GTNNAC 3 cut(s) 391, 572, 817
HpyAV CCTTC 3 cut(s) 719, 787, 877
HpyCH4III ACNGT 4 cut(s) 358, 850, 920, 1021
HpyCH4IV ACGT 4 cut(s) 27, 258, 373, 629
HpyCH4V TGCA 3 cut(s) 90, 311, 572
HpyF10VI GCNNNNNNNGC 6 cut(s) 221, 230, 269, 308, 602, 614
HpyF3I CTNAG 4 cut(s) 448, 481, 897, 965
HpySE526I ACGT 4 cut(s) 27, 258, 373, 629
Hsp92II CATG 6 cut(s) 46, 119, 463, 589, 828, 1037
HspAI GCGC 3 cut(s) 340, 452, 822
Kzo9I GATC 6 cut(s) 379, 534, 543, 742, 757, 780
LguI GCTCTTC 1 cut(s) 68
LmnI GCTCC 4 cut(s) 161, 212, 522, 981
Lsp1109I GCAGC 3 cut(s) 617, 620, 1012
LweI GCATC 2 cut(s) 193, 1012
MaeI CTAG 3 cut(s) 204, 221, 386
MaeII ACGT 4 cut(s) 27, 258, 373, 629
MaeIII GTNAC 6 cut(s) 28, 254, 352, 439, 670, 914
MalI GATC 6 cut(s) 381, 536, 545, 744, 759, 782
MbiI CCGCTC 1 cut(s) 525
MboI GATC 6 cut(s) 379, 534, 543, 742, 757, 780
MboII GAAGA 7 cut(s) 55, 76, 140, 219, 249, 341, 437
MflI RGATCY 1 cut(s) 757
MhlI GDGCHC 3 cut(s) 24, 482, 574
MluCI AATT 6 cut(s) 33, 642, 696, 710, 749, 838
MlyI GAGTC 2 cut(s) 243, 667
MmeI TCCRAC 1 cut(s) 637
MseI TTAA 4 cut(s) 12, 516, 581, 1038
MspI CCGG 5 cut(s) 159, 171, 558, 677, 788
MspR9I CCNGG 2 cut(s) 55, 678
MvaI CCWGG 1 cut(s) 55
MwoI GCNNNNNNNGC 6 cut(s) 221, 230, 269, 308, 602, 614
NciI CCSGG 1 cut(s) 678
NcoI CCATGG 1 cut(s) 459
NdeI CATATG 1 cut(s) 193
NdeII GATC 6 cut(s) 379, 534, 543, 742, 757, 780
NlaIII CATG 6 cut(s) 46, 119, 463, 589, 828, 1037
NlaIV GGNNCC 2 cut(s) 286, 493
NmeAIII GCCGAG 1 cut(s) 854
NmuCI GTSAC 3 cut(s) 254, 439, 670
NspI RCATGY 3 cut(s) 119, 589, 828
PaeI GCATGC 1 cut(s) 828
PciI ACATGT 1 cut(s) 115
PciSI GCTCTTC 1 cut(s) 68
PfeI GAWTC 2 cut(s) 361, 689
Pfl23II CGTACG 1 cut(s) 920
PfoI TCCNGGA 1 cut(s) 676
PkrI GCNGC 5 cut(s) 109, 607, 610, 802, 1002
PleI GAGTC 2 cut(s) 243, 667
PpsI GAGTC 2 cut(s) 243, 667
PscI ACATGT 1 cut(s) 115
PsiI TTATAA 1 cut(s) 932
Psp1406I AACGTT 1 cut(s) 27
Psp6I CCWGG 1 cut(s) 53
PspFI CCCAGC 1 cut(s) 398
PspGI CCWGG 1 cut(s) 53
PspLI CGTACG 1 cut(s) 920
PspN4I GGNNCC 2 cut(s) 286, 493
PstI CTGCAG 1 cut(s) 313
PstNI CAGNNNCTG 1 cut(s) 1006
PsuI RGATCY 1 cut(s) 757
RsaI GTAC 3 cut(s) 852, 922, 983
RsaNI GTAC 3 cut(s) 851, 921, 982
SapI GCTCTTC 1 cut(s) 68
SaqAI TTAA 4 cut(s) 12, 516, 581, 1038
SatI GCNGC 5 cut(s) 108, 606, 609, 801, 1001
Sau3AI GATC 6 cut(s) 379, 534, 543, 742, 757, 780
SchI GAGTC 2 cut(s) 243, 667
ScrFI CCNGG 2 cut(s) 55, 678
SduI GDGCHC 3 cut(s) 24, 482, 574
SfaNI GCATC 2 cut(s) 193, 1012
SfcI CTRYAG 1 cut(s) 309
SphI GCATGC 1 cut(s) 828
Sse9I AATT 6 cut(s) 33, 642, 696, 710, 749, 838
SsiI CCGC 7 cut(s) 105, 108, 138, 456, 525, 801, 941
SspMI CTAG 3 cut(s) 204, 221, 386
StyD4I CCNGG 2 cut(s) 53, 676
StyI CCWWGG 2 cut(s) 459, 720
TaaI ACNGT 4 cut(s) 358, 850, 920, 1021
TaiI ACGT 4 cut(s) 30, 261, 376, 632
TaqI TCGA 1 cut(s) 1024
TasI AATT 6 cut(s) 33, 642, 696, 710, 749, 838
TauI GCSGC 2 cut(s) 110, 803
TfiI GAWTC 2 cut(s) 361, 689
Tru1I TTAA 4 cut(s) 12, 516, 581, 1038
Tru9I TTAA 4 cut(s) 12, 516, 581, 1038
TscAI CASTG 3 cut(s) 313, 363, 439
TseFI GTSAC 3 cut(s) 254, 439, 670
TseI GCWGC 3 cut(s) 605, 608, 1000
Tsp45I GTSAC 3 cut(s) 254, 439, 670
TspGWI ACGGA 1 cut(s) 242
TspRI CASTG 3 cut(s) 313, 363, 439
VneI GTGCAC 1 cut(s) 570
XapI RAATTY 2 cut(s) 696, 710
XceI RCATGY 3 cut(s) 119, 589, 828
XcmI CCANNNNNNNNNTGG 2 cut(s) 811, 861
XspI CTAG 3 cut(s) 204, 221, 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.