Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0008035

Physical Location & Seq

Genomic Coordinates Forward (+)

24992492 .. 24996738

Gene Structure 4247 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1155700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1641 bp

ATGGCTAGTCAAGGCGGTTCTTCGAGGAGAAGTCTGTCATTGACGAACACATCGTTGCAGGACAAGAAGAAACCCTCTGATGCTAGTAACGGAGGCCCTGATTCTGCCCGAAAATCTTTCACAGCTTCTCGTTCAATGGGGCTAACTGGAGAGCGCACGGTGAAACGGTTGCGGCTATCAAAGGCTCTGACGGTACCTGAAAATACCAGTGTTCAAGAAGCTTGTCGTCGGATGGCTGCTCGTAGAGTCGATGCTTTGTTGTTAACGGACTCTAATGCATTGCTCTGTGGAATACTTACTGACAAGGATATAGCGACAAGGGTTATTTCTCGTGAGCTTGATCTTGAAGAAACCCTTGTTTCTAAAGTTATGACAAGGAACCCGATATTTGTTCTTTCAGACACTCTTGCTGTTGAGGCCCTTCAGAAAATGGTGCAAGGGAAATTCAGACATTTGCCTGTTGTGGAGAATGGAGAGGTCATTGCTTTACTTGATATAGCAAAGTGTTTATATGATGCCATTGCTCGAATGGAAAGAGCAGCTGAAAAGGGAAAGGCTATTGCTGCTGCTGTGGAAGGTGTTGAAAAACATTGGGGCACATCTGGTTCTGGTTCCAATACATTCGTTGAGACACTTCGAGAGCGGTTGTTTAGACCATCCTTGTCAACAATCATTCCGGAGAGTACAAAGGTTTTAATAGTTTCACCAACAGATACAGTTTTGATGACAACAAAGAAGATGCAGGAAATGCGAGTAAGCTCGGCAGTTGTGACGATTGACAACAAGCCACGAGGAATTCTTACCTCAAAAGATATCTTAATGCGCGTGATATCACAAAATCTTCCTCCGAAGACCACTCTTGTAGAGAAGGTCATGACTCCAAATCCAGAATGTGCTACAGTTGATACACCAATCGTTGATGCGTTGCATACCATGCATGATGGAAAGTTTTTACACATTCCTGTTCTCGACAGAGATGGAGCTGTAGTTGCCGTTGTTGATGTAATTCATATCACTCATGCTGCTGTGGCCACTGTTGGAAATACTGCTGGAGCGAGTAATGAGGCTGCAACCAATATGATGCAAAAGTTTTGGGACTCTGCCATGGCCTTGAGTCCCAATGATGATGACGATGAGTCTCGGAGTGAAGGTTCTTTGAAACTGGCTTCTGAGAGTGCAGTGACAGGGAGATCGCTTCCCTTTCCACCGTCCAATTTTCCAAATACGTTTGCTTTCAAACTTCAAGATAAAAGGGGCCGAATGCATAGATTCACTTGCAACATTCAGAATTTGACAGAACTTATAACCTCCATCATTCAGAGAATGGGAGATGACATTGACCGCAACAACCTTCCTCAAATTTTGTATGAAGATGAGGACCGTGATAAAGTTATATTGGCATCAGATAGTGATCTTGTAGCGGCTGTGGATCATGCAAAGCAAGTTGGTTGGAAGGGTTTGAGACTGCATTTGGATTATTCAGGAACACGCAGTCGCCGGCGAAGGGGTTCAGCTGCAGGAAATGTGGATTATGCATATGCAGATTCTGCATGGGCATCAGCATACAATGCTGCGGCAGCTGGGGCTGCTCTTGCTGTTGGCTTAGGCGTTTTAGCGTACCTGAAGAGATCCGGTAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBS PB1

Protein Analysis

547

Amino Acids

59.23

Weight (kDa)

8.78

Isoelectric Point (pI)

44.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBS	PF00571	60 - 107	6.1e-08	CBS domain
CBS	PF00571	120 - 164	1e-08	CBS domain
CBS	PF00571	228 - 276	3.1e-06	CBS domain
CBS	PF00571	288 - 341	1.5e-07	CBS domain
PB1	PF00564	409 - 492	2.2e-15	PB1 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008035)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63490 AT5G63490
fragaria_vesca	FvH4_5g08490 FvH4_5g08490 FvH4_5g08490 FvH4_5g08490 FvH4_5g08490 FvH4_5g08490
malus_domestica	MD06G1140500.v1.1 MD14G1155700.v1.1
prunus_persica	Prupe.5G149700_v2.0.a1
pyrus_communis	pycom14g12940
rosa_chinensis	RchiOBHm_Chr7g0189921
rosa_laevigata	RLG00000004577
rosa_multiflora	Rmu_sc0014588.1_g000001
rosa_roxburghii	Rroxscaffold_3G00265050
rosa_rugosa	Rorug06G0500900 Rorug06G0501000 Rorug06G0501100
rosa_samantha	Rh7AG106200 Rh7BG108900 Rh7CG111000 Rh7DG109400
rosa_wichuraiana	Rw7G009220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1304
Acc65I	GGTACC	1 cut(s)	193
AccB1I	GGYRCC	1 cut(s)	193
AccBSI	CCGCTC	1 cut(s)	643
AccII	CGCG	1 cut(s)	825
AccIII	TCCGGA	1 cut(s)	676
AciI	CCGC	6 cut(s)	15, 172, 643, 1342, 1421, 1574
AclWI	GGATC	2 cut(s)	1437, 1623
AcoI	YGGCCR	1 cut(s)	1029
AcsI	RAATTY	4 cut(s)	443, 795, 1288, 1359
AcuI	CTGAAG	1 cut(s)	407
AfaI	GTAC	3 cut(s)	195, 685, 1619
AfiI	CCNNNNNNNGG	1 cut(s)	1504
AgsI	TTSAA	7 cut(s)	135, 215, 347, 584, 1159, 1237, 1244
AhdI	GACNNNNNGTC	1 cut(s)	1135
AloI	GAACNNNNNNTCC	2 cut(s)	1135, 1167
AluBI	AGCT	8 cut(s)	125, 221, 337, 542, 759, 983, 1514, 1580
AluI	AGCT	8 cut(s)	125, 221, 337, 542, 759, 983, 1514, 1580
Alw26I	GTCTC	3 cut(s)	623, 1143, 1456
AlwI	GGATC	2 cut(s)	1437, 1623
AlwNI	CAGNNNCTG	1 cut(s)	1547
Aor13HI	TCCGGA	1 cut(s)	676
AoxI	GGCC	5 cut(s)	94, 417, 1029, 1107, 1255
ApoI	RAATTY	4 cut(s)	443, 795, 1288, 1359
Asp700I	GAANNNNTTC	1 cut(s)	1507
Asp718I	GGTACC	1 cut(s)	193
AspLEI	GCGC	2 cut(s)	156, 825
AspS9I	GGNCC	4 cut(s)	95, 418, 1255, 1378
AsuHPI	GGTGA	2 cut(s)	172, 696
AvaII	GGWCC	1 cut(s)	1378
BaeGI	GKGCMC	1 cut(s)	599
BalI	TGGCCA	1 cut(s)	1031
BanI	GGYRCC	1 cut(s)	193
BauI	CACGAG	2 cut(s)	330, 789
BbsI	GAAGAC	1 cut(s)	857
BccI	CCATC	5 cut(s)	226, 664, 935, 971, 1319
BceAI	ACGGC	1 cut(s)	977
BcoDI	GTCTC	3 cut(s)	623, 1143, 1456
BfaI	CTAG	2 cut(s)	6, 84
BfmI	CTRYAG	3 cut(s)	897, 984, 1515
Bme18I	GGWCC	1 cut(s)	1378
BmeRI	GACNNNNNGTC	1 cut(s)	1135
BmgT120I	GGNCC	4 cut(s)	95, 418, 1255, 1378
BmiI	GGNNCC	4 cut(s)	195, 380, 613, 1256
BmsI	GCATC	8 cut(s)	70, 241, 505, 729, 910, 1071, 1409, 1565
BpiI	GAAGAC	1 cut(s)	857
BpmI	CTGGAG	2 cut(s)	168, 1071
Bpu10I	CCTNAGC	1 cut(s)	1603
BpuEI	CTTGAG	1 cut(s)	1132
BsaBI	GATNNNNATC	1 cut(s)	1410
BsaJI	CCNNGG	1 cut(s)	1104
BsaWI	WCCGGW	2 cut(s)	676, 1631
BsaXI	ACNNNNNCTCC	4 cut(s)	972, 1002, 1135, 1165
Bsc4I	CCNNNNNNNGG	1 cut(s)	1504
Bse118I	RCCGGY	1 cut(s)	1497
Bse1I	ACTGG	3 cut(s)	151, 207, 1167
Bse3DI	GCAATG	3 cut(s)	278, 480, 519
Bse8I	GATNNNNATC	1 cut(s)	1410
BseAI	TCCGGA	1 cut(s)	676
BseDI	CCNNGG	1 cut(s)	1104
BseGI	GGATG	2 cut(s)	237, 656
BseJI	GATNNNNATC	1 cut(s)	1410
BseLI	CCNNNNNNNGG	1 cut(s)	1504
BseMI	GCAATG	3 cut(s)	278, 480, 519
BseMII	CTCAG	1 cut(s)	1161
BseNI	ACTGG	3 cut(s)	151, 207, 1167
BseRI	GAGGAG	1 cut(s)	40
BseSI	GKGCMC	1 cut(s)	599
BseYI	CCCAGC	1 cut(s)	1580
BsgI	GTGCAG	1 cut(s)	1197
Bsh1236I	CGCG	1 cut(s)	825
BshFI	GGCC	5 cut(s)	96, 419, 1031, 1109, 1257
BshNI	GGYRCC	1 cut(s)	193
BsiSI	CCGG	3 cut(s)	677, 1498, 1632
BslFI	GGGAC	2 cut(s)	1101, 1109
BslI	CCNNNNNNNGG	1 cut(s)	1504
BsmAI	GTCTC	3 cut(s)	623, 1143, 1456
BsmFI	GGGAC	2 cut(s)	1101, 1109
BsmI	GAATGC	1 cut(s)	1266
BsnI	GGCC	5 cut(s)	96, 419, 1031, 1109, 1257
Bsp1286I	GDGCHC	1 cut(s)	599
Bsp13I	TCCGGA	1 cut(s)	676
Bsp143I	GATC	5 cut(s)	340, 1190, 1411, 1429, 1628
Bsp19I	CCATGG	1 cut(s)	1104
BspACI	CCGC	6 cut(s)	15, 172, 643, 1342, 1421, 1574
BspANI	GGCC	5 cut(s)	96, 419, 1031, 1109, 1257
BspCNI	CTCAG	1 cut(s)	1162
BspEI	TCCGGA	1 cut(s)	676
BspFNI	CGCG	1 cut(s)	825
BspHI	TCATGA	1 cut(s)	873
BspLI	GGNNCC	4 cut(s)	195, 380, 613, 1256
BspMAI	CTGCAG	1 cut(s)	1519
BspPI	GGATC	2 cut(s)	1437, 1623
BspT107I	GGYRCC	1 cut(s)	193
BsrBI	CCGCTC	1 cut(s)	643
BsrDI	GCAATG	3 cut(s)	278, 480, 519
BsrFI	RCCGGY	1 cut(s)	1497
BsrI	ACTGG	3 cut(s)	151, 207, 1167
BssAI	RCCGGY	1 cut(s)	1497
BssECI	CCNNGG	1 cut(s)	1104
BssMI	GATC	5 cut(s)	340, 1190, 1411, 1429, 1628
BssSI	CACGAG	2 cut(s)	330, 789
BssT1I	CCWWGG	1 cut(s)	1104
Bst2BI	CACGAG	2 cut(s)	330, 789
Bst4CI	ACNGT	8 cut(s)	160, 168, 193, 718, 901, 1036, 1209, 1382
Bst6I	CTCTTC	1 cut(s)	1619
BstAPI	GCANNNNNTGC	4 cut(s)	748, 934, 1547, 1568
BstC8I	GCNNGC	1 cut(s)	1499
BstDEI	CTNAG	2 cut(s)	1170, 1603
BstDSI	CCRYGG	1 cut(s)	1104
BstF5I	GGATG	2 cut(s)	237, 656
BstFNI	CGCG	1 cut(s)	825
BstHHI	GCGC	2 cut(s)	156, 825
BstKTI	GATC	5 cut(s)	343, 1193, 1414, 1432, 1631
BstMAI	GTCTC	3 cut(s)	623, 1143, 1456
BstMBI	GATC	5 cut(s)	340, 1190, 1411, 1429, 1628
BstSFI	CTRYAG	3 cut(s)	897, 984, 1515
BstSLI	GKGCMC	1 cut(s)	599
BstUI	CGCG	1 cut(s)	825
BstV2I	GAAGAC	1 cut(s)	857
BstX2I	RGATCY	1 cut(s)	1628
BstYI	RGATCY	1 cut(s)	1628
BsuRI	GGCC	5 cut(s)	96, 419, 1031, 1109, 1257
BtgI	CCRYGG	1 cut(s)	1104
BtsCI	GGATG	2 cut(s)	237, 656
BtsI	GCAGTG	1 cut(s)	1185
BtsIMutI	CAGTG	3 cut(s)	214, 1032, 1185
Cac8I	GCNNGC	1 cut(s)	1499
CaiI	CAGNNNCTG	1 cut(s)	1547
CciI	TCATGA	1 cut(s)	873
CfoI	GCGC	2 cut(s)	156, 825
Cfr10I	RCCGGY	1 cut(s)	1497
Cfr13I	GGNCC	4 cut(s)	95, 418, 1255, 1378
Csp6I	GTAC	3 cut(s)	194, 684, 1618
CviAII	CATG	7 cut(s)	874, 934, 938, 1019, 1105, 1433, 1551
CviQI	GTAC	3 cut(s)	194, 684, 1618
DdeI	CTNAG	2 cut(s)	1170, 1603
DpnI	GATC	5 cut(s)	342, 1192, 1413, 1431, 1630
DpnII	GATC	5 cut(s)	340, 1190, 1411, 1429, 1628
DriI	GACNNNNNGTC	1 cut(s)	1135
EaeI	YGGCCR	1 cut(s)	1029
Eam1104I	CTCTTC	1 cut(s)	1619
Eam1105I	GACNNNNNGTC	1 cut(s)	1135
EarI	CTCTTC	1 cut(s)	1619
Eco130I	CCWWGG	1 cut(s)	1104
Eco32I	GATATC	2 cut(s)	814, 831
Eco47I	GGWCC	1 cut(s)	1378
Eco57I	CTGAAG	1 cut(s)	407
EcoO109I	RGGNCCY	2 cut(s)	95, 418
EcoRI	GAATTC	1 cut(s)	795
EcoRV	GATATC	2 cut(s)	814, 831
EcoT14I	CCWWGG	1 cut(s)	1104
EcoT22I	ATGCAT	4 cut(s)	280, 939, 1266, 1537
ErhI	CCWWGG	1 cut(s)	1104
FaeI	CATG	7 cut(s)	877, 937, 941, 1022, 1108, 1436, 1554
FalI	AAGNNNNNCTT	2 cut(s)	339, 371
FaqI	GGGAC	2 cut(s)	1101, 1109
FatI	CATG	7 cut(s)	873, 933, 937, 1018, 1104, 1432, 1550
FauNDI	CATATG	1 cut(s)	1537
FokI	GGATG	2 cut(s)	244, 643
FspBI	CTAG	2 cut(s)	6, 84
GlaI	GCGC	2 cut(s)	155, 824
GsaI	CCCAGC	1 cut(s)	1584
GsuI	CTGGAG	2 cut(s)	168, 1071
HaeIII	GGCC	5 cut(s)	96, 419, 1031, 1109, 1257
HapII	CCGG	3 cut(s)	677, 1498, 1632
HhaI	GCGC	2 cut(s)	156, 825
Hin1II	CATG	7 cut(s)	877, 937, 941, 1022, 1108, 1436, 1554
Hin6I	GCGC	2 cut(s)	154, 823
HinP1I	GCGC	2 cut(s)	154, 823
HincII	GTYRAC	2 cut(s)	264, 666
HindII	GTYRAC	2 cut(s)	264, 666
HindIII	AAGCTT	1 cut(s)	219
HinfI	GANTC	9 cut(s)	101, 246, 269, 877, 1097, 1114, 1136, 1269, 1544
HpaI	GTTAAC	1 cut(s)	264
HpaII	CCGG	3 cut(s)	677, 1498, 1632
HphI	GGTGA	2 cut(s)	172, 696
Hpy166II	GTNNAC	2 cut(s)	264, 666
Hpy8I	GTNNAC	2 cut(s)	264, 666
Hpy99I	CGWCG	1 cut(s)	231
HpyAV	CCTTC	7 cut(s)	431, 569, 862, 1142, 1361, 1447, 1497
HpyCH4III	ACNGT	8 cut(s)	160, 168, 193, 718, 901, 1036, 1209, 1382
HpyCH4IV	ACGT	1 cut(s)	1226
HpyF3I	CTNAG	2 cut(s)	1170, 1603
HpySE526I	ACGT	1 cut(s)	1226
Hsp92II	CATG	7 cut(s)	877, 937, 941, 1022, 1108, 1436, 1554
HspAI	GCGC	2 cut(s)	154, 823
Kpn2I	TCCGGA	1 cut(s)	676
KpnI	GGTACC	1 cut(s)	197
KroI	GCCGGC	1 cut(s)	1497
KroNI	GCCGGC	1 cut(s)	1499
KspAI	GTTAAC	1 cut(s)	264
Kzo9I	GATC	5 cut(s)	340, 1190, 1411, 1429, 1628
LmnI	GCTCC	2 cut(s)	980, 1052
LweI	GCATC	8 cut(s)	70, 241, 505, 729, 910, 1071, 1409, 1565
MaeI	CTAG	2 cut(s)	6, 84
MaeII	ACGT	1 cut(s)	1226
MaeIII	GTNAC	4 cut(s)	86, 769, 1180, 1634
MalI	GATC	5 cut(s)	342, 1192, 1413, 1431, 1630
MbiI	CCGCTC	1 cut(s)	643
MboI	GATC	5 cut(s)	340, 1190, 1411, 1429, 1628
MboII	GAAGA	8 cut(s)	12, 79, 359, 748, 833, 862, 1382, 1636
MflI	RGATCY	1 cut(s)	1628
MhlI	GDGCHC	1 cut(s)	599
MlsI	TGGCCA	1 cut(s)	1031
MluCI	AATT	6 cut(s)	443, 795, 1005, 1213, 1288, 1359
MluNI	TGGCCA	1 cut(s)	1031
MlyI	GAGTC	6 cut(s)	255, 263, 871, 1091, 1123, 1145
MmeI	TCCRAC	3 cut(s)	209, 1018, 1430
Mox20I	TGGCCA	1 cut(s)	1031
Mph1103I	ATGCAT	4 cut(s)	280, 939, 1266, 1537
MreI	CGCCGGCG	1 cut(s)	1497
MroI	TCCGGA	1 cut(s)	676
MroNI	GCCGGC	1 cut(s)	1497
MroXI	GAANNNNTTC	1 cut(s)	1507
MscI	TGGCCA	1 cut(s)	1031
MseI	TTAA	3 cut(s)	263, 695, 818
Msp20I	TGGCCA	1 cut(s)	1031
MspA1I	CMGCKG	3 cut(s)	542, 1514, 1580
MspI	CCGG	3 cut(s)	677, 1498, 1632
Mva1269I	GAATGC	1 cut(s)	1266
MvnI	CGCG	1 cut(s)	825
NaeI	GCCGGC	1 cut(s)	1499
NcoI	CCATGG	1 cut(s)	1104
NdeI	CATATG	1 cut(s)	1537
NdeII	GATC	5 cut(s)	340, 1190, 1411, 1429, 1628
NgoMIV	GCCGGC	1 cut(s)	1497
NlaIII	CATG	7 cut(s)	877, 937, 941, 1022, 1108, 1436, 1554
NlaIV	GGNNCC	4 cut(s)	195, 380, 613, 1256
NmeAIII	GCCGAG	1 cut(s)	740
NmuCI	GTSAC	2 cut(s)	769, 1180
NsiI	ATGCAT	4 cut(s)	280, 939, 1266, 1537
PagI	TCATGA	1 cut(s)	873
PcsI	WCGNNNNNNNCGW	1 cut(s)	50
PctI	GAATGC	1 cut(s)	1266
PdiI	GCCGGC	1 cut(s)	1499
PdmI	GAANNNNTTC	1 cut(s)	1507
PfeI	GAWTC	3 cut(s)	101, 1269, 1544
PleI	GAGTC	6 cut(s)	254, 263, 871, 1091, 1122, 1144
PpsI	GAGTC	6 cut(s)	254, 263, 871, 1091, 1122, 1144
PsiI	TTATAA	1 cut(s)	1304
PspFI	CCCAGC	1 cut(s)	1580
PspN4I	GGNNCC	4 cut(s)	195, 380, 613, 1256
PspPI	GGNCC	4 cut(s)	95, 418, 1255, 1378
PstI	CTGCAG	1 cut(s)	1519
PstNI	CAGNNNCTG	1 cut(s)	1547
PsuI	RGATCY	1 cut(s)	1628
PvuII	CAGCTG	3 cut(s)	542, 1514, 1580
RsaI	GTAC	3 cut(s)	195, 685, 1619
RsaNI	GTAC	3 cut(s)	194, 684, 1618
SaqAI	TTAA	3 cut(s)	263, 695, 818
Sau3AI	GATC	5 cut(s)	340, 1190, 1411, 1429, 1628
Sau96I	GGNCC	4 cut(s)	95, 418, 1255, 1378
SchI	GAGTC	6 cut(s)	255, 263, 871, 1091, 1123, 1145
SduI	GDGCHC	1 cut(s)	599
SfaNI	GCATC	8 cut(s)	70, 241, 505, 729, 910, 1071, 1409, 1565
SfcI	CTRYAG	3 cut(s)	897, 984, 1515
SgrAI	CRCCGGYG	1 cut(s)	1497
SinI	GGWCC	1 cut(s)	1378
SmlI	CTYRAG	1 cut(s)	1111
SmoI	CTYRAG	1 cut(s)	1111
Sse9I	AATT	6 cut(s)	443, 795, 1005, 1213, 1288, 1359
SsiI	CCGC	6 cut(s)	15, 172, 643, 1342, 1421, 1574
SspMI	CTAG	2 cut(s)	6, 84
StyI	CCWWGG	1 cut(s)	1104
TaaI	ACNGT	8 cut(s)	160, 168, 193, 718, 901, 1036, 1209, 1382
TaiI	ACGT	1 cut(s)	1229
TaqI	TCGA	5 cut(s)	23, 249, 526, 637, 969
TasI	AATT	6 cut(s)	443, 795, 1005, 1213, 1288, 1359
TatI	WGTACW	1 cut(s)	683
TauI	GCSGC	3 cut(s)	175, 1424, 1577
TfiI	GAWTC	3 cut(s)	101, 1269, 1544
Tru1I	TTAA	3 cut(s)	263, 695, 818
Tru9I	TTAA	3 cut(s)	263, 695, 818
TscAI	CASTG	3 cut(s)	214, 1039, 1185
TseFI	GTSAC	2 cut(s)	769, 1180
Tsp45I	GTSAC	2 cut(s)	769, 1180
TspDTI	ATGAA	2 cut(s)	998, 1383
TspGWI	ACGGA	2 cut(s)	105, 281
TspRI	CASTG	3 cut(s)	214, 1039, 1185
VpaK11BI	GGWCC	1 cut(s)	1378
XapI	RAATTY	4 cut(s)	443, 795, 1288, 1359
XcmI	CCANNNNNNNNNTGG	1 cut(s)	526
XmnI	GAANNNNTTC	1 cut(s)	1507
XspI	CTAG	2 cut(s)	6, 84
Zsp2I	ATGCAT	4 cut(s)	280, 939, 1266, 1537

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.