RchiOBHm_Chr7g0189921

CBS domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0008035
Physical Location & Seq
Forward (+)
9223557 .. 9227792
4236 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16962

Sequence Viewer

Length: 1659 bp
ATGGCTAGTCAAGGCGGTTCTTCGAGGAGAAGTCTGTCTATGACGAACGCATCGTTGCAGGAGAAGAAGAAATCCTCTGAGAGTCATCATGGAGGTGGAGGCCATGATGCCGGCAGAAAATCGCTTTCGGCGTCTCGTCCTCCAGTGGGGTTAACCGGAGAGCGCACGGTAAAACGTTTGCGGCTATCAAAGGCTCTAACGGTACCTGAAAGTACTAGTATTCAAGAAGCTTGTCGTCGGATGGCTGCTCGTAGAGTTGATGCGTTGTTGTTAACCGACTCTAATGCATTGCTTTGTGGAATACTTACCGACAAGGATATAGCAACGAGAGTTATTGCACGCGAGCTTAATCTTGAGGAAACACTTGTTTCTAAAGTTATGACGAGGAACCCAATATTTGTACTTTCTGACACTCTTGCAGTCGAAGCCCTTCAGAAGATGGTTCAAGGAAAATTCAGACATTTGCCTGTGGTGGAGAATGGAGAGGTAATTGCTTTGCTAGATATAGCAAAGTGTTTGTATGATGCTATTGCTCGAATGGAAAGGGCAGCTGAAAAGGGAAAGGCTATTGCTGCTGCTGTGGAAGGTGTTGAAAAACATTGGGGCAGCTCTAATTCTGATGCAGGTTCCAATACATTTGTTGAAACACTTCGTGAAAAGTTGTTTAGGCCATCTTTGTCTACAATCATTCCGGAGAATACAAAGGTCTTGACAGTTTCGCCAACAGATACAGTTTTGGCAACAGCAAAGAAGATGCAGGAGATGCGAATTAGTTCTGCAGTTGTGACAGTTGATAACAAGCCACGAGGAATTCTTACTTCTAAGGATATTTTGATGCGGGTCGTATCACAAAATCTTCCTCCAAAGACCACCCTTGTAGAGAAGGTCATGACTCCAAATCCAGAATGTGCCTCGGTTGATACACCAATTGTTGATGCACTGCACACTATGCATGATGGAAAATTTTTGCACCTGCCTGTAGTAGACAGAGATGGAGGTATAGTTGCTGTTGTTGATGTAATTCATATCACTCATGCTGCTGTGGCCACTGTTGGAAATAGTGCTGGAGTGTTTAATGAGACCGCAAGTACTATGATGCAGAAGTTTTGGGATTCTGCCATGGCCTTAAGTCCAAATGATGAAGATGAGGAATGTCGAAGTGAAAATTCTTTAAGACTGGCTTCTGAGGGAGCAGAGACTAGGGGGTCACATCCCTTTCCATCACCAATTTTTCCAAATACTTTTGCTTTCAAGATTCAGGATAAGAAAGGCCGAATGCACAGATTTACTTGCAATATTCAGAGTTTGACTGATCTTATAACTTCCATTATTCATAGAATGGGAGATGACATTGACCGCCACAACCTTCCTCAGATATTGTATGAAGACGAAGATCGTGATAAAGTAATATTGGCATCTGATAGTGATCTCGCTGCAGCCGTGGACCATGCCAGGCAAGTTGGTTGGAAGGGCTTGAGATTGCATTTAGATTTCTCAAAAACACCTGGTCGTCGTCGAAGGGCTACAGCTGGTGGAACCATGGATTATGCTTATGCAGATTCTGCTTGGGCGTCAGCATACAGTGCCGTGGCAGCTGGGGCTGCAATTGCTGCTGGCTTTGGCGTTTTGGCATACTTGAAGAGAGCCGGCAATAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBS PB1

Protein Analysis

552

Amino Acids

59.83

Weight (kDa)

8.59

Isoelectric Point (pI)

44.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBS PF00571 63 - 110 1e-07 CBS domain
CBS PF00571 123 - 167 1.1e-08 CBS domain
CBS PF00571 233 - 281 1.9e-08 CBS domain
CBS PF00571 293 - 346 7.8e-09 CBS domain
PB1 PF00564 414 - 497 2.1e-15 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1319
AarI CACCTGC 1 cut(s) 981
AasI GACNNNNNNGTC 1 cut(s) 1204
Acc36I ACCTGC 2 cut(s) 614, 981
Acc65I GGTACC 1 cut(s) 202
AccB1I GGYRCC 1 cut(s) 202
AccI GTMKAC 2 cut(s) 680, 984
AccII CGCG 1 cut(s) 342
AccIII TCCGGA 1 cut(s) 691
AciI CCGC 5 cut(s) 15, 181, 838, 1083, 1357
AclI AACGTT 1 cut(s) 175
AcoI YGGCCR 1 cut(s) 1044
AcsI RAATTY 4 cut(s) 452, 810, 962, 1165
AcuI CTGAAG 1 cut(s) 416
AcyI GRCGYC 2 cut(s) 131, 1571
AdeI CACNNNGTG 1 cut(s) 653
AfaI GTAC 4 cut(s) 204, 214, 402, 1090
AfiI CCNNNNNNNGG 1 cut(s) 146
AflII CTTAAG 1 cut(s) 1126
AgsI TTSAA 6 cut(s) 224, 446, 593, 644, 1252, 1639
AhlI ACTAGT 1 cut(s) 215
AjnI CCWGG 2 cut(s) 1451, 1504
AluBI AGCT 6 cut(s) 230, 346, 551, 609, 1529, 1595
AluI AGCT 6 cut(s) 230, 346, 551, 609, 1529, 1595
Alw26I GTCTC 3 cut(s) 138, 1073, 1190
AlwNI CAGNNNCTG 1 cut(s) 1562
Aor13HI TCCGGA 1 cut(s) 691
AoxI GGCC 5 cut(s) 100, 668, 1044, 1122, 1270
ApoI RAATTY 4 cut(s) 452, 810, 962, 1165
Asp700I GAANNNNTTC 3 cut(s) 429, 648, 772
Asp718I GGTACC 1 cut(s) 202
AspLEI GCGC 1 cut(s) 165
AspS9I GGNCC 1 cut(s) 1444
AsuHPI GGTGA 1 cut(s) 1215
AvaII GGWCC 1 cut(s) 1444
BalI TGGCCA 1 cut(s) 1046
BanI GGYRCC 1 cut(s) 202
BauI CACGAG 1 cut(s) 804
BbsI GAAGAC 1 cut(s) 1392
BccI CCATC 6 cut(s) 235, 433, 679, 950, 986, 1228
BceAI ACGGC 2 cut(s) 1424, 1571
BcgI CGANNNNNNTGC 2 cut(s) 266, 300
BciT130I CCWGG 2 cut(s) 1453, 1506
BcoDI GTCTC 3 cut(s) 138, 1073, 1190
BcuI ACTAGT 1 cut(s) 215
BfaI CTAG 4 cut(s) 6, 216, 500, 1200
BfmI CTRYAG 4 cut(s) 777, 978, 1434, 1524
BfrI CTTAAG 1 cut(s) 1126
BfuAI ACCTGC 2 cut(s) 614, 981
BmcAI AGTACT 2 cut(s) 214, 1090
Bme1390I CCNGG 2 cut(s) 1453, 1506
Bme18I GGWCC 1 cut(s) 1444
BmgT120I GGNCC 1 cut(s) 1444
BmiI GGNNCC 4 cut(s) 204, 389, 628, 1537
BmrFI CCNGG 2 cut(s) 1453, 1506
BpiI GAAGAC 1 cut(s) 1392
BpmI CTGGAG 2 cut(s) 126, 1086
BpuEI CTTGAG 2 cut(s) 374, 1495
BsaBI GATNNNNATC 1 cut(s) 1425
BsaHI GRCGYC 2 cut(s) 131, 1571
BsaI GGTCTC 1 cut(s) 1073
BsaJI CCNNGG 5 cut(s) 912, 1119, 1440, 1539, 1587
BsaWI WCCGGW 2 cut(s) 155, 691
BsaXI ACNNNNNCTCC 2 cut(s) 987, 1017
Bsc4I CCNNNNNNNGG 1 cut(s) 146
Bse118I RCCGGY 2 cut(s) 110, 1646
Bse1I ACTGG 2 cut(s) 143, 1182
Bse3DI GCAATG 1 cut(s) 287
Bse8I GATNNNNATC 1 cut(s) 1425
BseAI TCCGGA 1 cut(s) 691
BseBI CCWGG 2 cut(s) 1453, 1506
BseDI CCNNGG 5 cut(s) 912, 1119, 1440, 1539, 1587
BseGI GGATG 2 cut(s) 246, 1210
BseJI GATNNNNATC 1 cut(s) 1425
BseLI CCNNNNNNNGG 1 cut(s) 146
BseMI GCAATG 1 cut(s) 287
BseMII CTCAG 3 cut(s) 69, 1176, 1385
BseNI ACTGG 2 cut(s) 143, 1182
BseRI GAGGAG 1 cut(s) 40
BseYI CCCAGC 1 cut(s) 1595
BsgI GTGCAG 1 cut(s) 926
Bsh1236I CGCG 1 cut(s) 342
BshFI GGCC 5 cut(s) 102, 670, 1046, 1124, 1272
BshNI GGYRCC 1 cut(s) 202
BsiSI CCGG 4 cut(s) 111, 156, 692, 1647
BslI CCNNNNNNNGG 1 cut(s) 146
BsmAI GTCTC 3 cut(s) 138, 1073, 1190
BsmBI CGTCTC 1 cut(s) 138
BsmI GAATGC 1 cut(s) 1281
BsnI GGCC 5 cut(s) 102, 670, 1046, 1124, 1272
Bso31I GGTCTC 1 cut(s) 1073
Bsp13I TCCGGA 1 cut(s) 691
Bsp143I GATC 3 cut(s) 1312, 1393, 1426
Bsp19I CCATGG 2 cut(s) 1119, 1539
BspACI CCGC 5 cut(s) 15, 181, 838, 1083, 1357
BspANI GGCC 5 cut(s) 102, 670, 1046, 1124, 1272
BspCNI CTCAG 3 cut(s) 70, 1177, 1384
BspEI TCCGGA 1 cut(s) 691
BspFNI CGCG 1 cut(s) 342
BspHI TCATGA 1 cut(s) 888
BspLI GGNNCC 4 cut(s) 204, 389, 628, 1537
BspMAI CTGCAG 2 cut(s) 781, 1438
BspMI ACCTGC 2 cut(s) 614, 981
BspT107I GGYRCC 1 cut(s) 202
BspTI CTTAAG 1 cut(s) 1126
BspTNI GGTCTC 1 cut(s) 1073
BsrDI GCAATG 1 cut(s) 287
BsrFI RCCGGY 2 cut(s) 110, 1646
BsrI ACTGG 2 cut(s) 143, 1182
BssAI RCCGGY 2 cut(s) 110, 1646
BssECI CCNNGG 5 cut(s) 912, 1119, 1440, 1539, 1587
BssMI GATC 3 cut(s) 1312, 1393, 1426
BssNI GRCGYC 2 cut(s) 131, 1571
BssSI CACGAG 1 cut(s) 804
BssT1I CCWWGG 2 cut(s) 1119, 1539
Bst2BI CACGAG 1 cut(s) 804
Bst2UI CCWGG 2 cut(s) 1453, 1506
Bst4CI ACNGT 7 cut(s) 169, 202, 715, 733, 790, 1051, 1583
Bst6I CTCTTC 1 cut(s) 1634
BstACI GRCGYC 2 cut(s) 131, 1571
BstAFI CTTAAG 1 cut(s) 1126
BstAPI GCANNNNNTGC 5 cut(s) 763, 949, 1562, 1583, 1610
BstC8I GCNNGC 5 cut(s) 112, 340, 344, 1615, 1648
BstDEI CTNAG 4 cut(s) 78, 822, 1185, 1371
BstDSI CCRYGG 4 cut(s) 1119, 1440, 1539, 1587
BstF5I GGATG 2 cut(s) 246, 1210
BstFNI CGCG 1 cut(s) 342
BstHHI GCGC 1 cut(s) 165
BstKTI GATC 3 cut(s) 1315, 1396, 1429
BstMAI GTCTC 3 cut(s) 138, 1073, 1190
BstMBI GATC 3 cut(s) 1312, 1393, 1426
BstNI CCWGG 2 cut(s) 1453, 1506
BstSCI CCNGG 2 cut(s) 1451, 1504
BstSFI CTRYAG 4 cut(s) 777, 978, 1434, 1524
BstUI CGCG 1 cut(s) 342
BstV2I GAAGAC 1 cut(s) 1392
BsuRI GGCC 5 cut(s) 102, 670, 1046, 1124, 1272
BtgI CCRYGG 4 cut(s) 1119, 1440, 1539, 1587
BtsCI GGATG 2 cut(s) 246, 1210
BtsI GCAGTG 1 cut(s) 938
BtsIMutI CAGTG 4 cut(s) 150, 938, 1047, 1588
BveI ACCTGC 2 cut(s) 614, 981
Cac8I GCNNGC 5 cut(s) 112, 340, 344, 1615, 1648
CaiI CAGNNNCTG 1 cut(s) 1562
CciI TCATGA 1 cut(s) 888
CfoI GCGC 1 cut(s) 165
Cfr10I RCCGGY 2 cut(s) 110, 1646
Cfr13I GGNCC 1 cut(s) 1444
CseI GACGC 2 cut(s) 120, 1560
CsiI ACCWGGT 1 cut(s) 1504
Csp6I GTAC 4 cut(s) 203, 213, 401, 1089
CviAII CATG 8 cut(s) 89, 104, 889, 953, 1034, 1120, 1448, 1540
CviQI GTAC 4 cut(s) 203, 213, 401, 1089
DdeI CTNAG 4 cut(s) 78, 822, 1185, 1371
DpnI GATC 3 cut(s) 1314, 1395, 1428
DpnII GATC 3 cut(s) 1312, 1393, 1426
DraIII CACNNNGTG 1 cut(s) 653
DrdI GACNNNNNNGTC 1 cut(s) 1204
DseDI GACNNNNNNGTC 1 cut(s) 1204
EaeI YGGCCR 1 cut(s) 1044
Eam1104I CTCTTC 1 cut(s) 1634
EarI CTCTTC 1 cut(s) 1634
Eco130I CCWWGG 2 cut(s) 1119, 1539
Eco31I GGTCTC 1 cut(s) 1073
Eco47I GGWCC 1 cut(s) 1444
Eco57I CTGAAG 1 cut(s) 416
EcoRI GAATTC 1 cut(s) 810
EcoRII CCWGG 2 cut(s) 1451, 1504
EcoT14I CCWWGG 2 cut(s) 1119, 1539
EcoT22I ATGCAT 2 cut(s) 289, 954
ErhI CCWWGG 2 cut(s) 1119, 1539
Esp3I CGTCTC 1 cut(s) 138
FaeI CATG 8 cut(s) 92, 107, 892, 956, 1037, 1123, 1451, 1543
FalI AAGNNNNNCTT 2 cut(s) 1165, 1197
FatI CATG 8 cut(s) 88, 103, 888, 952, 1033, 1119, 1447, 1539
FauI CCCGC 1 cut(s) 831
FblI GTMKAC 2 cut(s) 680, 984
FokI GGATG 2 cut(s) 253, 1197
FspBI CTAG 4 cut(s) 6, 216, 500, 1200
GlaI GCGC 1 cut(s) 164
GsaI CCCAGC 1 cut(s) 1599
GsuI CTGGAG 2 cut(s) 126, 1086
HaeIII GGCC 5 cut(s) 102, 670, 1046, 1124, 1272
HapII CCGG 4 cut(s) 111, 156, 692, 1647
HgaI GACGC 2 cut(s) 120, 1560
HhaI GCGC 1 cut(s) 165
Hin1I GRCGYC 2 cut(s) 131, 1571
Hin1II CATG 8 cut(s) 92, 107, 892, 956, 1037, 1123, 1451, 1543
Hin6I GCGC 1 cut(s) 163
HinP1I GCGC 1 cut(s) 163
HincII GTYRAC 2 cut(s) 153, 273
HindII GTYRAC 2 cut(s) 153, 273
HindIII AAGCTT 1 cut(s) 228
HinfI GANTC 6 cut(s) 82, 278, 892, 1112, 1255, 1559
HpaI GTTAAC 2 cut(s) 153, 273
HpaII CCGG 4 cut(s) 111, 156, 692, 1647
HphI GGTGA 1 cut(s) 1215
Hpy166II GTNNAC 5 cut(s) 153, 273, 681, 985, 1444
Hpy8I GTNNAC 5 cut(s) 153, 273, 681, 985, 1444
Hpy99I CGWCG 3 cut(s) 240, 1515, 1518
HpyAV CCTTC 6 cut(s) 440, 578, 877, 1376, 1462, 1512
HpyCH4III ACNGT 7 cut(s) 169, 202, 715, 733, 790, 1051, 1583
HpyCH4IV ACGT 1 cut(s) 175
HpyF3I CTNAG 4 cut(s) 78, 822, 1185, 1371
HpySE526I ACGT 1 cut(s) 175
Hsp92I GRCGYC 2 cut(s) 131, 1571
Hsp92II CATG 8 cut(s) 92, 107, 892, 956, 1037, 1123, 1451, 1543
HspAI GCGC 1 cut(s) 163
Kpn2I TCCGGA 1 cut(s) 691
KpnI GGTACC 1 cut(s) 206
KroI GCCGGC 2 cut(s) 110, 1646
KroNI GCCGGC 2 cut(s) 112, 1648
KspAI GTTAAC 2 cut(s) 153, 273
Kzo9I GATC 3 cut(s) 1312, 1393, 1426
LmnI GCTCC 1 cut(s) 1190
MabI ACCWGGT 1 cut(s) 1504
MaeI CTAG 4 cut(s) 6, 216, 500, 1200
MaeII ACGT 1 cut(s) 175
MaeIII GTNAC 2 cut(s) 784, 1206
MalI GATC 3 cut(s) 1314, 1395, 1428
MboI GATC 3 cut(s) 1312, 1393, 1426
MfeI CAATTG 2 cut(s) 927, 1605
MlsI TGGCCA 1 cut(s) 1046
MluNI TGGCCA 1 cut(s) 1046
MlyI GAGTC 3 cut(s) 91, 272, 886
MmeI TCCRAC 3 cut(s) 218, 1033, 1445
Mox20I TGGCCA 1 cut(s) 1046
Mph1103I ATGCAT 2 cut(s) 289, 954
MroI TCCGGA 1 cut(s) 691
MroNI GCCGGC 2 cut(s) 110, 1646
MroXI GAANNNNTTC 3 cut(s) 429, 648, 772
MscI TGGCCA 1 cut(s) 1046
MseI TTAA 6 cut(s) 152, 272, 348, 1074, 1127, 1172
MslI CAYNNNNRTG 1 cut(s) 93
Msp20I TGGCCA 1 cut(s) 1046
MspA1I CMGCKG 3 cut(s) 551, 1529, 1595
MspCI CTTAAG 1 cut(s) 1126
MspI CCGG 4 cut(s) 111, 156, 692, 1647
MspR9I CCNGG 2 cut(s) 1453, 1506
MunI CAATTG 2 cut(s) 927, 1605
Mva1269I GAATGC 1 cut(s) 1281
MvaI CCWGG 2 cut(s) 1453, 1506
MvnI CGCG 1 cut(s) 342
NaeI GCCGGC 2 cut(s) 112, 1648
NcoI CCATGG 2 cut(s) 1119, 1539
NdeII GATC 3 cut(s) 1312, 1393, 1426
NgoMIV GCCGGC 2 cut(s) 110, 1646
NlaIII CATG 8 cut(s) 92, 107, 892, 956, 1037, 1123, 1451, 1543
NlaIV GGNNCC 4 cut(s) 204, 389, 628, 1537
NmuCI GTSAC 2 cut(s) 784, 1206
NsiI ATGCAT 2 cut(s) 289, 954
PagI TCATGA 1 cut(s) 888
PaqCI CACCTGC 1 cut(s) 981
PcsI WCGNNNNNNNCGW 3 cut(s) 50, 128, 1437
PctI GAATGC 1 cut(s) 1281
PdiI GCCGGC 2 cut(s) 112, 1648
PdmI GAANNNNTTC 3 cut(s) 429, 648, 772
PfeI GAWTC 3 cut(s) 1112, 1255, 1559
PleI GAGTC 3 cut(s) 90, 272, 886
PpsI GAGTC 3 cut(s) 90, 272, 886
PsiI TTATAA 1 cut(s) 1319
Psp1406I AACGTT 1 cut(s) 175
Psp6I CCWGG 2 cut(s) 1451, 1504
PspFI CCCAGC 1 cut(s) 1595
PspGI CCWGG 2 cut(s) 1451, 1504
PspN4I GGNNCC 4 cut(s) 204, 389, 628, 1537
PspPI GGNCC 1 cut(s) 1444
PstI CTGCAG 2 cut(s) 781, 1438
PstNI CAGNNNCTG 1 cut(s) 1562
PvuII CAGCTG 3 cut(s) 551, 1529, 1595
RsaI GTAC 4 cut(s) 204, 214, 402, 1090
RsaNI GTAC 4 cut(s) 203, 213, 401, 1089
RseI CAYNNNNRTG 1 cut(s) 93
SaqAI TTAA 6 cut(s) 152, 272, 348, 1074, 1127, 1172
Sau3AI GATC 3 cut(s) 1312, 1393, 1426
Sau96I GGNCC 1 cut(s) 1444
ScaI AGTACT 2 cut(s) 214, 1090
SchI GAGTC 3 cut(s) 91, 272, 886
ScrFI CCNGG 2 cut(s) 1453, 1506
SexAI ACCWGGT 1 cut(s) 1504
SfcI CTRYAG 4 cut(s) 777, 978, 1434, 1524
SinI GGWCC 1 cut(s) 1444
SmiMI CAYNNNNRTG 1 cut(s) 93
SmlI CTYRAG 3 cut(s) 353, 1126, 1474
SmoI CTYRAG 3 cut(s) 353, 1126, 1474
SpeI ACTAGT 1 cut(s) 215
SsiI CCGC 5 cut(s) 15, 181, 838, 1083, 1357
SspI AATATT 3 cut(s) 396, 1297, 1410
SspMI CTAG 4 cut(s) 6, 216, 500, 1200
StyD4I CCNGG 2 cut(s) 1451, 1504
StyI CCWWGG 2 cut(s) 1119, 1539
TaaI ACNGT 7 cut(s) 169, 202, 715, 733, 790, 1051, 1583
TaiI ACGT 1 cut(s) 178
TaqI TCGA 5 cut(s) 23, 423, 535, 1156, 1516
TatI WGTACW 3 cut(s) 212, 400, 1088
TauI GCSGC 1 cut(s) 184
TfiI GAWTC 3 cut(s) 1112, 1255, 1559
Tru1I TTAA 6 cut(s) 152, 272, 348, 1074, 1127, 1172
Tru9I TTAA 6 cut(s) 152, 272, 348, 1074, 1127, 1172
TscAI CASTG 4 cut(s) 150, 945, 1054, 1588
TseFI GTSAC 2 cut(s) 784, 1206
Tsp45I GTSAC 2 cut(s) 784, 1206
TspDTI ATGAA 4 cut(s) 1013, 1155, 1322, 1398
TspRI CASTG 4 cut(s) 150, 945, 1054, 1588
Vha464I CTTAAG 1 cut(s) 1126
VpaK11BI GGWCC 1 cut(s) 1444
XapI RAATTY 4 cut(s) 452, 810, 962, 1165
XmiI GTMKAC 2 cut(s) 680, 984
XmnI GAANNNNTTC 3 cut(s) 429, 648, 772
XspI CTAG 4 cut(s) 6, 216, 500, 1200
ZrmI AGTACT 2 cut(s) 214, 1090
Zsp2I ATGCAT 2 cut(s) 289, 954
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.