MD14G1155800.v1.1

Transferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0017651
Physical Location & Seq
Reverse (-)
24997548 .. 24998822
1275 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1155800.v1.1.491

Sequence Viewer

Length: 1275 bp
ATGGAACTCGAAATAGTATCTAGAGAAATCATCAAACCCTCCTCTCCAACTCCATCTCACCTAAGAACTTTCAACTTCTGCTACTTTAATAAGATGGCTGGTCGTATGTACGTGCCGGTGGTTCTTTTCTACAACCCTGCGGGTGGCGCATGTGCTGTTGAAAAATCCAATCTGCTCAAGAAGTCTCTCTCGGAAACACTAGCCTTGTACTACCCATTTGCTGGAAGGATCGTAGATGCAGAATATATTGACTGTACTGATGAGGGAGTGACATATTTGGAAGCGAAAATCCATGCCAAGTTGTCAGAAGTTCTCCGAAATCCTGACAATGAAACATTAGACCTTTTATTTGCAGACAATTTGCAATGGGAGGACACAAATCTGAGCACTTTACTTGCCGTTCAGGTTAGCTACTTTGAGTGTGGAGGAATGGCAATAAGCATGTGCATGTCACACAAACTTGGCGATGCGGCCACCCTGATCACCTTCATCAAAGACTGGTCTGCCATGGCTTCAAACTCAGGTGAACATGTGATCCCTCTATTCGATTTAACCTCAATGTTCCCACAGGACGACCTACCGATTGGTAAGGAACATGTTAATGATAAAGGGAAGCTTGCCACAAGAAGGTTTGTATTTGATGCCCTAAAAATTGCCGCCCTCAAGGCCATTGTTGCTCAGAAAGTGCAAAACCCTACAAGGGTAGAGGTTGTGACTGCACTACTATTTAAGTGTGCAAGCTCAACCTCTGGTGGACGAGCAGTCCTTTGCCAAAATATCAACTTGCGTTCGAGGCTCGTCCCTCCGTTGCCGGAAAATTTTGTAGGAAATTTGGCTTGGTTCTTTCCAGTACCAAAACTAGAGGAGGATGGTGAGATAGAGTTGCCTGGCTTCGTGGGACAAATGAAAGAGGCTTTGGCTGAATTCTGCAATACATATGCGAGCAAGTTTAGAGGCAAAGAATGGCGCTTGAGCATTAAGCAACACATGGAGAAGGCTAAGGAATTGTTTGGGAGCAACACAGCTAAATATACATGTAGCAGCTGGTGTAGGCTTCGAATATACGAAGTGGACTTTGGGTGGGGAAAGCCGGTGTGGGCGAGGATCGGCAGCCGTGCGGGGAAGAATGTCAGTATTCTAATGGATACAAGAAGTGGGGATGGGATTGAGGCCTTTGTGACGTTGGAAGAGCAAGACATGGCAAAGTTTGAGCGTAATGAGGAGTTACTAGCATTTGCTACTTTTAACCCAAATCCCATTGAGGAACTCTCGTGA

Protein Analysis

425

Amino Acids

47.46

Weight (kDa)

5.6

Isoelectric Point (pI)

40.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 1 - 409 2.3e-79 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017651)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24660
malus_domestica MD14G1155800.v1.1
pyrus_communis pycom14g12950
rosa_chinensis RchiOBHm_Chr4g0431761
rosa_laevigata RLG00000006892
rosa_multiflora Rmu_sc0001459.1_g000022
rosa_roxburghii Rroxscaffold_5G00373160
rosa_rugosa Rorug04G0252200
rosa_samantha Rh4AG308600 Rh4BG316400
rosa_wichuraiana Rw4G026770 Rw4G026840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 221
AciI CCGC 4 cut(s) 140, 470, 657, 1118
AclWI GGATC 3 cut(s) 236, 529, 1112
AcoI YGGCCR 1 cut(s) 471
AcsI RAATTY 3 cut(s) 817, 829, 923
AfaI GTAC 4 cut(s) 110, 209, 256, 852
AfiI CCNNNNNNNGG 4 cut(s) 143, 221, 627, 700
AflIII ACRYGT 3 cut(s) 529, 595, 1034
AgsI TTSAA 3 cut(s) 73, 161, 516
AhdI GACNNNNNGTC 1 cut(s) 761
AjnI CCWGG 1 cut(s) 886
AjuI GAANNNNNNNTTGG 6 cut(s) 820, 852, 899, 931, 1059, 1091
AloI GAACNNNNNNTCC 2 cut(s) 519, 551
AluBI AGCT 5 cut(s) 411, 616, 741, 1025, 1044
AluI AGCT 5 cut(s) 411, 616, 741, 1025, 1044
Alw21I GWGCWC 1 cut(s) 389
Alw26I GTCTC 1 cut(s) 189
AlwI GGATC 3 cut(s) 236, 529, 1112
AoxI GGCC 3 cut(s) 471, 666, 1170
ApeKI GCWGC 2 cut(s) 1041, 1110
ApoI RAATTY 3 cut(s) 817, 829, 923
ArsI GACNNNNNNTTYG 2 cut(s) 332, 364
AspLEI GCGC 2 cut(s) 149, 969
AsuHPI GGTGA 4 cut(s) 50, 475, 536, 884
AsuII TTCGAA 1 cut(s) 1057
BauI CACGAG 1 cut(s) 1270
Bbv12I GWGCWC 1 cut(s) 389
BbvI GCAGC 2 cut(s) 1053, 1122
BccI CCATC 4 cut(s) 61, 88, 863, 1154
BceAI ACGGC 2 cut(s) 383, 1098
BciT130I CCWGG 1 cut(s) 888
BciVI GTATCC 1 cut(s) 1138
BclI TGATCA 1 cut(s) 480
BcoDI GTCTC 1 cut(s) 189
BfaI CTAG 4 cut(s) 21, 200, 860, 1229
BfoI RGCGCY 1 cut(s) 970
BfuI GTATCC 1 cut(s) 1138
BglI GCCNNNNNGGC 1 cut(s) 665
BisI GCNGC 4 cut(s) 471, 657, 1042, 1111
BlsI GCNGC 4 cut(s) 472, 658, 1043, 1112
Bme1390I CCNGG 1 cut(s) 888
BmeRI GACNNNNNGTC 1 cut(s) 761
BmrFI CCNGG 1 cut(s) 888
BmsI GCATC 3 cut(s) 226, 457, 631
BplI GAGNNNNNCTC 1 cut(s) 1253
Bpu10I CCTNAGC 1 cut(s) 999
Bpu14I TTCGAA 1 cut(s) 1057
BpuEI CTTGAG 3 cut(s) 161, 647, 991
BsaAI YACGTR 1 cut(s) 112
BsaJI CCNNGG 1 cut(s) 507
Bsc4I CCNNNNNNNGG 4 cut(s) 143, 221, 627, 700
Bse118I RCCGGY 2 cut(s) 115, 1090
Bse1I ACTGG 2 cut(s) 503, 848
Bse3DI GCAATG 1 cut(s) 371
BseBI CCWGG 1 cut(s) 888
BseDI CCNNGG 1 cut(s) 507
BseGI GGATG 2 cut(s) 874, 1165
BseLI CCNNNNNNNGG 4 cut(s) 143, 221, 627, 700
BseMI GCAATG 1 cut(s) 371
BseMII CTCAG 3 cut(s) 374, 534, 692
BseNI ACTGG 2 cut(s) 503, 848
BseRI GAGGAG 3 cut(s) 31, 878, 1235
BseXI GCAGC 2 cut(s) 1053, 1122
BsgI GTGCAG 1 cut(s) 702
BshFI GGCC 3 cut(s) 473, 668, 1172
BsiHKAI GWGCWC 1 cut(s) 389
BsiSI CCGG 3 cut(s) 116, 812, 1091
BslFI GGGAC 2 cut(s) 785, 912
BslI CCNNNNNNNGG 4 cut(s) 143, 221, 627, 700
BsmAI GTCTC 1 cut(s) 189
BsmFI GGGAC 2 cut(s) 785, 912
BsnI GGCC 3 cut(s) 473, 668, 1172
Bsp119I TTCGAA 1 cut(s) 1057
Bsp1286I GDGCHC 1 cut(s) 389
Bsp143I GATC 4 cut(s) 228, 480, 534, 1104
Bsp19I CCATGG 1 cut(s) 507
BspACI CCGC 4 cut(s) 140, 470, 657, 1118
BspANI GGCC 3 cut(s) 473, 668, 1172
BspCNI CTCAG 3 cut(s) 375, 533, 691
BspPI GGATC 3 cut(s) 236, 529, 1112
BspQI GCTCTTC 1 cut(s) 1182
BspT104I TTCGAA 1 cut(s) 1057
BsrDI GCAATG 1 cut(s) 371
BsrFI RCCGGY 2 cut(s) 115, 1090
BsrI ACTGG 2 cut(s) 503, 848
BssAI RCCGGY 2 cut(s) 115, 1090
BssECI CCNNGG 1 cut(s) 507
BssMI GATC 4 cut(s) 228, 480, 534, 1104
BssSI CACGAG 1 cut(s) 1270
BssT1I CCWWGG 1 cut(s) 507
Bst2BI CACGAG 1 cut(s) 1270
Bst2UI CCWGG 1 cut(s) 888
Bst4CI ACNGT 1 cut(s) 254
Bst6I CTCTTC 1 cut(s) 1182
BstBAI YACGTR 1 cut(s) 112
BstBI TTCGAA 1 cut(s) 1057
BstC8I GCNNGC 3 cut(s) 618, 739, 943
BstDEI CTNAG 5 cut(s) 62, 383, 520, 678, 999
BstDSI CCRYGG 1 cut(s) 507
BstF5I GGATG 2 cut(s) 874, 1165
BstH2I RGCGCY 1 cut(s) 970
BstHHI GCGC 2 cut(s) 149, 969
BstKTI GATC 4 cut(s) 231, 483, 537, 1107
BstMAI GTCTC 1 cut(s) 189
BstMBI GATC 4 cut(s) 228, 480, 534, 1104
BstMWI GCNNNNNNNGC 4 cut(s) 146, 665, 674, 793
BstNI CCWGG 1 cut(s) 888
BstNSI RCATGY 6 cut(s) 153, 445, 451, 533, 599, 1038
BstSCI CCNGG 1 cut(s) 886
BstV1I GCAGC 2 cut(s) 1053, 1122
BsuI GTATCC 1 cut(s) 1138
BsuRI GGCC 3 cut(s) 473, 668, 1172
BtgI CCRYGG 1 cut(s) 507
BtgZI GCGATG 1 cut(s) 480
BtsCI GGATG 2 cut(s) 874, 1165
Cac8I GCNNGC 3 cut(s) 618, 739, 943
CfoI GCGC 2 cut(s) 149, 969
Cfr10I RCCGGY 2 cut(s) 115, 1090
Csp6I GTAC 4 cut(s) 109, 208, 255, 851
CviQI GTAC 4 cut(s) 109, 208, 255, 851
DdeI CTNAG 5 cut(s) 62, 383, 520, 678, 999
DpnI GATC 4 cut(s) 230, 482, 536, 1106
DpnII GATC 4 cut(s) 228, 480, 534, 1104
DriI GACNNNNNGTC 1 cut(s) 761
EaeI YGGCCR 1 cut(s) 471
Eam1104I CTCTTC 1 cut(s) 1182
Eam1105I GACNNNNNGTC 1 cut(s) 761
EarI CTCTTC 1 cut(s) 1182
Eco130I CCWWGG 1 cut(s) 507
Eco147I AGGCCT 1 cut(s) 1172
EcoRI GAATTC 1 cut(s) 923
EcoRII CCWGG 1 cut(s) 886
EcoT14I CCWWGG 1 cut(s) 507
ErhI CCWWGG 1 cut(s) 507
FalI AAGNNNNNCTT 2 cut(s) 600, 632
FaqI GGGAC 2 cut(s) 785, 912
FauI CCCGC 2 cut(s) 133, 1111
FauNDI CATATG 1 cut(s) 937
FbaI TGATCA 1 cut(s) 480
Fnu4HI GCNGC 4 cut(s) 471, 657, 1042, 1111
FokI GGATG 2 cut(s) 881, 1172
Fsp4HI GCNGC 4 cut(s) 471, 657, 1042, 1111
FspBI CTAG 4 cut(s) 21, 200, 860, 1229
GlaI GCGC 2 cut(s) 148, 968
GluI GCNGC 4 cut(s) 471, 657, 1042, 1111
HaeII RGCGCY 1 cut(s) 970
HaeIII GGCC 3 cut(s) 473, 668, 1172
HapII CCGG 3 cut(s) 116, 812, 1091
HhaI GCGC 2 cut(s) 149, 969
Hin6I GCGC 2 cut(s) 147, 967
HinP1I GCGC 2 cut(s) 147, 967
HindIII AAGCTT 1 cut(s) 614
HpaII CCGG 3 cut(s) 116, 812, 1091
HphI GGTGA 4 cut(s) 50, 475, 536, 884
Hpy166II GTNNAC 3 cut(s) 527, 755, 1072
Hpy188I TCNGA 5 cut(s) 193, 307, 317, 384, 681
Hpy188III TCNNGA 4 cut(s) 21, 178, 323, 1272
Hpy8I GTNNAC 3 cut(s) 527, 755, 1072
HpyAV CCTTC 4 cut(s) 219, 496, 621, 988
HpyCH4III ACNGT 1 cut(s) 254
HpyCH4IV ACGT 2 cut(s) 111, 1181
HpyCH4V TGCA 8 cut(s) 239, 353, 364, 447, 688, 719, 737, 930
HpyF10VI GCNNNNNNNGC 4 cut(s) 146, 665, 674, 793
HpyF3I CTNAG 5 cut(s) 62, 383, 520, 678, 999
HpySE526I ACGT 2 cut(s) 111, 1181
HspAI GCGC 2 cut(s) 147, 967
Ksp22I TGATCA 1 cut(s) 480
Kzo9I GATC 4 cut(s) 228, 480, 534, 1104
LguI GCTCTTC 1 cut(s) 1182
LmnI GCTCC 1 cut(s) 1014
Lsp1109I GCAGC 2 cut(s) 1053, 1122
LweI GCATC 3 cut(s) 226, 457, 631
MaeI CTAG 4 cut(s) 21, 200, 860, 1229
MaeII ACGT 2 cut(s) 111, 1181
MaeIII GTNAC 5 cut(s) 268, 450, 712, 1177, 1224
MalI GATC 4 cut(s) 230, 482, 536, 1106
MboI GATC 4 cut(s) 228, 480, 534, 1104
MboII GAAGA 2 cut(s) 1135, 1199
MhlI GDGCHC 1 cut(s) 389
MluCI AATT 6 cut(s) 358, 651, 817, 829, 923, 1004
MmeI TCCRAC 2 cut(s) 71, 1164
MseI TTAA 6 cut(s) 87, 551, 600, 729, 978, 1245
MslI CAYNNNNRTG 2 cut(s) 446, 600
MspA1I CMGCKG 1 cut(s) 1044
MspI CCGG 3 cut(s) 116, 812, 1091
MspR9I CCNGG 1 cut(s) 888
MvaI CCWGG 1 cut(s) 888
MwoI GCNNNNNNNGC 4 cut(s) 146, 665, 674, 793
NcoI CCATGG 1 cut(s) 507
NdeI CATATG 1 cut(s) 937
NdeII GATC 4 cut(s) 228, 480, 534, 1104
NmuCI GTSAC 4 cut(s) 268, 450, 712, 1177
NspI RCATGY 6 cut(s) 153, 445, 451, 533, 599, 1038
NspV TTCGAA 1 cut(s) 1057
PceI AGGCCT 1 cut(s) 1172
PciI ACATGT 3 cut(s) 529, 595, 1034
PciSI GCTCTTC 1 cut(s) 1182
PflMI CCANNNNNTGG 1 cut(s) 221
PkrI GCNGC 4 cut(s) 472, 658, 1043, 1112
Ppu21I YACGTR 1 cut(s) 112
PscI ACATGT 3 cut(s) 529, 595, 1034
Psp6I CCWGG 1 cut(s) 886
PspGI CCWGG 1 cut(s) 886
PvuII CAGCTG 1 cut(s) 1044
RsaI GTAC 4 cut(s) 110, 209, 256, 852
RsaNI GTAC 4 cut(s) 109, 208, 255, 851
RseI CAYNNNNRTG 2 cut(s) 446, 600
SapI GCTCTTC 1 cut(s) 1182
SaqAI TTAA 6 cut(s) 87, 551, 600, 729, 978, 1245
SatI GCNGC 4 cut(s) 471, 657, 1042, 1111
Sau3AI GATC 4 cut(s) 228, 480, 534, 1104
ScrFI CCNGG 1 cut(s) 888
SduI GDGCHC 1 cut(s) 389
SfaNI GCATC 3 cut(s) 226, 457, 631
SfuI TTCGAA 1 cut(s) 1057
SmiMI CAYNNNNRTG 2 cut(s) 446, 600
SmlI CTYRAG 3 cut(s) 176, 662, 970
SmoI CTYRAG 3 cut(s) 176, 662, 970
Sse9I AATT 6 cut(s) 358, 651, 817, 829, 923, 1004
SseBI AGGCCT 1 cut(s) 1172
SsiI CCGC 4 cut(s) 140, 470, 657, 1118
SspMI CTAG 4 cut(s) 21, 200, 860, 1229
StuI AGGCCT 1 cut(s) 1172
StyD4I CCNGG 1 cut(s) 886
StyI CCWWGG 1 cut(s) 507
TaaI ACNGT 1 cut(s) 254
TaiI ACGT 2 cut(s) 114, 1184
TaqI TCGA 4 cut(s) 9, 546, 791, 1057
TasI AATT 6 cut(s) 358, 651, 817, 829, 923, 1004
TatI WGTACW 2 cut(s) 207, 254
TauI GCSGC 2 cut(s) 473, 659
Tru1I TTAA 6 cut(s) 87, 551, 600, 729, 978, 1245
Tru9I TTAA 6 cut(s) 87, 551, 600, 729, 978, 1245
TseFI GTSAC 4 cut(s) 268, 450, 712, 1177
TseI GCWGC 2 cut(s) 1041, 1110
Tsp45I GTSAC 4 cut(s) 268, 450, 712, 1177
TspDTI ATGAA 3 cut(s) 345, 478, 920
TspGWI ACGGA 1 cut(s) 795
Van91I CCANNNNNTGG 1 cut(s) 221
XapI RAATTY 3 cut(s) 817, 829, 923
XbaI TCTAGA 1 cut(s) 20
XceI RCATGY 6 cut(s) 153, 445, 451, 533, 599, 1038
XspI CTAG 4 cut(s) 21, 200, 860, 1229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.