pycom14g12950

Transferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0017651
Physical Location & Seq
Reverse (-)
15968649 .. 15969923
1275 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g12950.1

Sequence Viewer

Length: 1275 bp
ATGGAACTCGAAATAGTATCTAGAGAAATCATCAAACCCTCCTCTCCAACTCCATCTCACCTAAGAACTTTCAACTTCTGCTACTTTGATAAGATGGCTGGTCGTATGTACGTGCCGGTGGTTCTTTTCTACAACCCTGCGGCTGGCGCATGTGCTGTTGAAAAATCCAATCTGCTCAAGAAGTCTCTGTCGGAAACACTAGCCTTGTACTACCCATTTGCTGGAAGGGTCGTAGATGCAAAATATATTGACTGTACTGATGAGGGAGTGACATATTTGGAAGCGAAAATCCATGCCAAGTTGTCAGAAGTTCTTCGAAATCCTGACAATGAAACATTAGACCTTCTATTTGCAGACAATTTGCAATGGGAGGACACAAATCTGAGCACTTTACTGGCCGTTCAGGTTAGCTACTTTGAGTGTGGAGGAATGGCAATAAGCATGTGCATGTCACACAAACTTGGCGATGCCGCCACCCTGATCACCTTCATCAAAGACTGGGCTGCCATGGCTTCAAACTCAGGTGAACATTTGATCCCTCTATTCGATATAACCTCAATGTTCCCACAGGACGACATACCGATTGGTAAGGAACATGTTAATGATAAAGGGAAGCTTGCCACAAGAAGGTTTGTATTTGATGCCCTAAAAATTGCCGCCCTCAAGGCCATTGTTGCTCAGAAAGTGCATAACCCTACAAAGGTAGAGGTTGTGACTGCACTACTATTTAAGTGTGCAAGCTCAACCTCTGGTGGACGAGCAGTCCTTTGCCAAAATATCAACTTGCGTTCGAGGCTCGTCCCTCCCATGCAGGAAAATTTTGTGGGAAATTTGGCTTGGTTCTTTTCAGTCCCAAAACTAGAGGAGGATGGTGAGATAGAGCTGCCTGGCTTCGTGGGACAAATGAAAGAGGCTTTGGCTGAATTCTGCAATACATATGCGAGCAAGTTTAGAGGCAAAGAATGGTGCTTGAGCATTCAGCAACACATGAAGAAGGCTAAGGAATTGTCTGGGAGCGGCACAGCTAAATATATATGTAGCAGCTGGTGTAGGCTTCCGATATACGAAGTGGACTTTGGGTGGGGAAAGCCGGTGTGGGCGAGGATCGGCAGCCGGGCGGGGAAGAATGTCAGTATTCTACTGGATACCAGAAGCGGGGATGGGATTGAGGCCTTTGTGACGTTGGAAGAGCAAGACATGGCAAAGTTTGAGCGTAATGAGGAGTTACTAGCATTTGCTACTTTTAACCCAAATCCCATTGAGGAACTCTCGTAA

Protein Analysis

425

Amino Acids

47.24

Weight (kDa)

5.66

Isoelectric Point (pI)

38.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 1 - 409 1.2e-80 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017651)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24660
malus_domestica MD14G1155800.v1.1
pyrus_communis pycom14g12950
rosa_chinensis RchiOBHm_Chr4g0431761
rosa_laevigata RLG00000006892
rosa_multiflora Rmu_sc0001459.1_g000022
rosa_roxburghii Rroxscaffold_5G00373160
rosa_rugosa Rorug04G0252200
rosa_samantha Rh4AG308600 Rh4BG316400
rosa_wichuraiana Rw4G026770 Rw4G026840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 221
AccBSI CCGCTC 1 cut(s) 1017
AciI CCGC 6 cut(s) 140, 471, 657, 1017, 1118, 1155
AclWI GGATC 2 cut(s) 529, 1112
AcoI YGGCCR 1 cut(s) 396
AcsI RAATTY 3 cut(s) 817, 829, 923
AfaI GTAC 3 cut(s) 110, 209, 256
AfiI CCNNNNNNNGG 5 cut(s) 143, 221, 627, 700, 1155
AflIII ACRYGT 1 cut(s) 595
AgsI TTSAA 3 cut(s) 73, 161, 516
AhdI GACNNNNNGTC 1 cut(s) 761
AjnI CCWGG 1 cut(s) 886
AjuI GAANNNNNNNTTGG 6 cut(s) 820, 852, 899, 931, 1059, 1091
AloI GAACNNNNNNTCC 2 cut(s) 519, 551
AluBI AGCT 6 cut(s) 411, 616, 741, 883, 1025, 1044
AluI AGCT 6 cut(s) 411, 616, 741, 883, 1025, 1044
Alw21I GWGCWC 1 cut(s) 389
Alw26I GTCTC 1 cut(s) 189
AlwI GGATC 2 cut(s) 529, 1112
AoxI GGCC 3 cut(s) 396, 666, 1170
ApeKI GCWGC 4 cut(s) 503, 883, 1041, 1110
ApoI RAATTY 3 cut(s) 817, 829, 923
ArsI GACNNNNNNTTYG 2 cut(s) 332, 364
Asp700I GAANNNNTTC 1 cut(s) 312
AspLEI GCGC 1 cut(s) 149
AsuC2I CCSGG 1 cut(s) 1115
AsuHPI GGTGA 4 cut(s) 50, 475, 536, 884
AsuII TTCGAA 1 cut(s) 316
Bbv12I GWGCWC 1 cut(s) 389
BbvI GCAGC 4 cut(s) 490, 870, 1053, 1122
BccI CCATC 4 cut(s) 61, 88, 863, 1154
BceAI ACGGC 1 cut(s) 383
BciT130I CCWGG 1 cut(s) 888
BciVI GTATCC 1 cut(s) 1138
BclI TGATCA 1 cut(s) 480
BcnI CCSGG 1 cut(s) 1115
BcoDI GTCTC 1 cut(s) 189
BfaI CTAG 4 cut(s) 21, 200, 860, 1229
BfuI GTATCC 1 cut(s) 1138
BglI GCCNNNNNGGC 1 cut(s) 665
BisI GCNGC 8 cut(s) 141, 471, 504, 657, 884, 1018, 1042, 1111
BlsI GCNGC 8 cut(s) 142, 472, 505, 658, 885, 1019, 1043, 1112
Bme1390I CCNGG 2 cut(s) 888, 1115
BmeRI GACNNNNNGTC 1 cut(s) 761
BmrFI CCNGG 2 cut(s) 888, 1115
BmrI ACTGGG 1 cut(s) 508
BmsI GCATC 3 cut(s) 226, 457, 631
BmuI ACTGGG 1 cut(s) 508
BplI GAGNNNNNCTC 1 cut(s) 1253
Bpu10I CCTNAGC 1 cut(s) 999
Bpu14I TTCGAA 1 cut(s) 316
BpuEI CTTGAG 3 cut(s) 161, 647, 991
BpuMI CCSGG 1 cut(s) 1115
BsaAI YACGTR 1 cut(s) 112
BsaJI CCNNGG 1 cut(s) 507
Bsc4I CCNNNNNNNGG 5 cut(s) 143, 221, 627, 700, 1155
Bse118I RCCGGY 2 cut(s) 115, 1090
Bse1I ACTGG 3 cut(s) 399, 503, 1146
Bse3DI GCAATG 1 cut(s) 371
BseBI CCWGG 1 cut(s) 888
BseDI CCNNGG 1 cut(s) 507
BseGI GGATG 2 cut(s) 874, 1165
BseLI CCNNNNNNNGG 5 cut(s) 143, 221, 627, 700, 1155
BseMI GCAATG 1 cut(s) 371
BseMII CTCAG 3 cut(s) 374, 534, 692
BseNI ACTGG 3 cut(s) 399, 503, 1146
BseRI GAGGAG 3 cut(s) 31, 878, 1235
BseXI GCAGC 4 cut(s) 490, 870, 1053, 1122
BsgI GTGCAG 1 cut(s) 702
BshFI GGCC 3 cut(s) 398, 668, 1172
BsiHKAI GWGCWC 1 cut(s) 389
BsiSI CCGG 3 cut(s) 116, 1091, 1114
BslFI GGGAC 3 cut(s) 785, 836, 912
BslI CCNNNNNNNGG 5 cut(s) 143, 221, 627, 700, 1155
BsmAI GTCTC 1 cut(s) 189
BsmFI GGGAC 3 cut(s) 785, 836, 912
BsmI GAATGC 1 cut(s) 975
BsnI GGCC 3 cut(s) 398, 668, 1172
Bsp119I TTCGAA 1 cut(s) 316
Bsp1286I GDGCHC 1 cut(s) 389
Bsp143I GATC 3 cut(s) 480, 534, 1104
Bsp19I CCATGG 1 cut(s) 507
BspACI CCGC 6 cut(s) 140, 471, 657, 1017, 1118, 1155
BspANI GGCC 3 cut(s) 398, 668, 1172
BspCNI CTCAG 3 cut(s) 375, 533, 691
BspPI GGATC 2 cut(s) 529, 1112
BspQI GCTCTTC 1 cut(s) 1182
BspT104I TTCGAA 1 cut(s) 316
BsrBI CCGCTC 1 cut(s) 1017
BsrDI GCAATG 1 cut(s) 371
BsrFI RCCGGY 2 cut(s) 115, 1090
BsrI ACTGG 3 cut(s) 399, 503, 1146
BssAI RCCGGY 2 cut(s) 115, 1090
BssECI CCNNGG 1 cut(s) 507
BssMI GATC 3 cut(s) 480, 534, 1104
BssT1I CCWWGG 1 cut(s) 507
Bst2UI CCWGG 1 cut(s) 888
Bst4CI ACNGT 1 cut(s) 254
Bst6I CTCTTC 1 cut(s) 1182
BstBAI YACGTR 1 cut(s) 112
BstBI TTCGAA 1 cut(s) 316
BstC8I GCNNGC 4 cut(s) 145, 618, 739, 943
BstDEI CTNAG 5 cut(s) 62, 383, 520, 678, 999
BstDSI CCRYGG 1 cut(s) 507
BstF5I GGATG 2 cut(s) 874, 1165
BstHHI GCGC 1 cut(s) 149
BstKTI GATC 3 cut(s) 483, 537, 1107
BstMAI GTCTC 1 cut(s) 189
BstMBI GATC 3 cut(s) 480, 534, 1104
BstMWI GCNNNNNNNGC 5 cut(s) 146, 509, 665, 674, 793
BstNI CCWGG 1 cut(s) 888
BstNSI RCATGY 4 cut(s) 153, 445, 451, 599
BstSCI CCNGG 2 cut(s) 886, 1113
BstV1I GCAGC 4 cut(s) 490, 870, 1053, 1122
BsuI GTATCC 1 cut(s) 1138
BsuRI GGCC 3 cut(s) 398, 668, 1172
BtgI CCRYGG 1 cut(s) 507
BtgZI GCGATG 1 cut(s) 480
BtsCI GGATG 2 cut(s) 874, 1165
Cac8I GCNNGC 4 cut(s) 145, 618, 739, 943
CfoI GCGC 1 cut(s) 149
Cfr10I RCCGGY 2 cut(s) 115, 1090
Csp6I GTAC 3 cut(s) 109, 208, 255
CviAII CATG 9 cut(s) 150, 293, 442, 448, 508, 596, 808, 988, 1198
CviQI GTAC 3 cut(s) 109, 208, 255
DdeI CTNAG 5 cut(s) 62, 383, 520, 678, 999
DpnI GATC 3 cut(s) 482, 536, 1106
DpnII GATC 3 cut(s) 480, 534, 1104
DriI GACNNNNNGTC 1 cut(s) 761
EaeI YGGCCR 1 cut(s) 396
Eam1104I CTCTTC 1 cut(s) 1182
Eam1105I GACNNNNNGTC 1 cut(s) 761
EarI CTCTTC 1 cut(s) 1182
Eco130I CCWWGG 1 cut(s) 507
Eco147I AGGCCT 1 cut(s) 1172
EcoRI GAATTC 1 cut(s) 923
EcoRII CCWGG 1 cut(s) 886
EcoT14I CCWWGG 1 cut(s) 507
ErhI CCWWGG 1 cut(s) 507
FaeI CATG 9 cut(s) 153, 296, 445, 451, 511, 599, 811, 991, 1201
FalI AAGNNNNNCTT 2 cut(s) 600, 632
FaqI GGGAC 3 cut(s) 785, 836, 912
FatI CATG 9 cut(s) 149, 292, 441, 447, 507, 595, 807, 987, 1197
FauI CCCGC 2 cut(s) 1111, 1148
FauNDI CATATG 1 cut(s) 937
FbaI TGATCA 1 cut(s) 480
Fnu4HI GCNGC 8 cut(s) 141, 471, 504, 657, 884, 1018, 1042, 1111
FokI GGATG 2 cut(s) 881, 1172
Fsp4HI GCNGC 8 cut(s) 141, 471, 504, 657, 884, 1018, 1042, 1111
FspBI CTAG 4 cut(s) 21, 200, 860, 1229
GlaI GCGC 1 cut(s) 148
GluI GCNGC 8 cut(s) 141, 471, 504, 657, 884, 1018, 1042, 1111
HaeIII GGCC 3 cut(s) 398, 668, 1172
HapII CCGG 3 cut(s) 116, 1091, 1114
HhaI GCGC 1 cut(s) 149
Hin1II CATG 9 cut(s) 153, 296, 445, 451, 511, 599, 811, 991, 1201
Hin6I GCGC 1 cut(s) 147
HinP1I GCGC 1 cut(s) 147
HindIII AAGCTT 1 cut(s) 614
HpaII CCGG 3 cut(s) 116, 1091, 1114
HphI GGTGA 4 cut(s) 50, 475, 536, 884
Hpy166II GTNNAC 3 cut(s) 527, 755, 1072
Hpy188I TCNGA 5 cut(s) 193, 307, 384, 681, 1059
Hpy188III TCNNGA 3 cut(s) 21, 178, 323
Hpy8I GTNNAC 3 cut(s) 527, 755, 1072
HpyAV CCTTC 5 cut(s) 219, 353, 496, 621, 988
HpyCH4III ACNGT 1 cut(s) 254
HpyCH4IV ACGT 2 cut(s) 111, 1181
HpyCH4V TGCA 9 cut(s) 239, 353, 364, 447, 688, 719, 737, 811, 930
HpyF10VI GCNNNNNNNGC 5 cut(s) 146, 509, 665, 674, 793
HpyF3I CTNAG 5 cut(s) 62, 383, 520, 678, 999
HpySE526I ACGT 2 cut(s) 111, 1181
Hsp92II CATG 9 cut(s) 153, 296, 445, 451, 511, 599, 811, 991, 1201
HspAI GCGC 1 cut(s) 147
Ksp22I TGATCA 1 cut(s) 480
Kzo9I GATC 3 cut(s) 480, 534, 1104
LguI GCTCTTC 1 cut(s) 1182
LmnI GCTCC 1 cut(s) 1014
Lsp1109I GCAGC 4 cut(s) 490, 870, 1053, 1122
LweI GCATC 3 cut(s) 226, 457, 631
MaeI CTAG 4 cut(s) 21, 200, 860, 1229
MaeII ACGT 2 cut(s) 111, 1181
MaeIII GTNAC 5 cut(s) 268, 450, 712, 1177, 1224
MalI GATC 3 cut(s) 482, 536, 1106
MbiI CCGCTC 1 cut(s) 1017
MboI GATC 3 cut(s) 480, 534, 1104
MboII GAAGA 4 cut(s) 305, 1003, 1135, 1199
MhlI GDGCHC 1 cut(s) 389
MluCI AATT 6 cut(s) 358, 651, 817, 829, 923, 1004
MmeI TCCRAC 3 cut(s) 71, 171, 1164
MroXI GAANNNNTTC 1 cut(s) 312
MseI TTAA 3 cut(s) 600, 729, 1245
MslI CAYNNNNRTG 2 cut(s) 446, 600
MspA1I CMGCKG 1 cut(s) 1044
MspI CCGG 3 cut(s) 116, 1091, 1114
MspR9I CCNGG 2 cut(s) 888, 1115
Mva1269I GAATGC 1 cut(s) 975
MvaI CCWGG 1 cut(s) 888
MwoI GCNNNNNNNGC 5 cut(s) 146, 509, 665, 674, 793
NciI CCSGG 1 cut(s) 1115
NcoI CCATGG 1 cut(s) 507
NdeI CATATG 1 cut(s) 937
NdeII GATC 3 cut(s) 480, 534, 1104
NlaIII CATG 9 cut(s) 153, 296, 445, 451, 511, 599, 811, 991, 1201
NmuCI GTSAC 4 cut(s) 268, 450, 712, 1177
NspI RCATGY 4 cut(s) 153, 445, 451, 599
NspV TTCGAA 1 cut(s) 316
PceI AGGCCT 1 cut(s) 1172
PciI ACATGT 1 cut(s) 595
PciSI GCTCTTC 1 cut(s) 1182
PctI GAATGC 1 cut(s) 975
PdmI GAANNNNTTC 1 cut(s) 312
PflMI CCANNNNNTGG 1 cut(s) 221
PkrI GCNGC 8 cut(s) 142, 472, 505, 658, 885, 1019, 1043, 1112
Ppu21I YACGTR 1 cut(s) 112
PscI ACATGT 1 cut(s) 595
Psp6I CCWGG 1 cut(s) 886
PspGI CCWGG 1 cut(s) 886
PvuII CAGCTG 1 cut(s) 1044
RsaI GTAC 3 cut(s) 110, 209, 256
RsaNI GTAC 3 cut(s) 109, 208, 255
RseI CAYNNNNRTG 2 cut(s) 446, 600
SapI GCTCTTC 1 cut(s) 1182
SaqAI TTAA 3 cut(s) 600, 729, 1245
SatI GCNGC 8 cut(s) 141, 471, 504, 657, 884, 1018, 1042, 1111
Sau3AI GATC 3 cut(s) 480, 534, 1104
ScrFI CCNGG 2 cut(s) 888, 1115
SduI GDGCHC 1 cut(s) 389
SfaNI GCATC 3 cut(s) 226, 457, 631
SfuI TTCGAA 1 cut(s) 316
SmiMI CAYNNNNRTG 2 cut(s) 446, 600
SmlI CTYRAG 3 cut(s) 176, 662, 970
SmoI CTYRAG 3 cut(s) 176, 662, 970
Sse9I AATT 6 cut(s) 358, 651, 817, 829, 923, 1004
SseBI AGGCCT 1 cut(s) 1172
SsiI CCGC 6 cut(s) 140, 471, 657, 1017, 1118, 1155
SspMI CTAG 4 cut(s) 21, 200, 860, 1229
StuI AGGCCT 1 cut(s) 1172
StyD4I CCNGG 2 cut(s) 886, 1113
StyI CCWWGG 1 cut(s) 507
TaaI ACNGT 1 cut(s) 254
TaiI ACGT 2 cut(s) 114, 1184
TaqI TCGA 4 cut(s) 9, 316, 546, 791
TasI AATT 6 cut(s) 358, 651, 817, 829, 923, 1004
TatI WGTACW 2 cut(s) 207, 254
TauI GCSGC 4 cut(s) 143, 473, 659, 1020
Tru1I TTAA 3 cut(s) 600, 729, 1245
Tru9I TTAA 3 cut(s) 600, 729, 1245
TseFI GTSAC 4 cut(s) 268, 450, 712, 1177
TseI GCWGC 4 cut(s) 503, 883, 1041, 1110
Tsp45I GTSAC 4 cut(s) 268, 450, 712, 1177
TspDTI ATGAA 4 cut(s) 345, 478, 920, 1004
Van91I CCANNNNNTGG 1 cut(s) 221
XapI RAATTY 3 cut(s) 817, 829, 923
XbaI TCTAGA 1 cut(s) 20
XceI RCATGY 4 cut(s) 153, 445, 451, 599
XmnI GAANNNNTTC 1 cut(s) 312
XspI CTAG 4 cut(s) 21, 200, 860, 1229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.