Rroxscaffold_5G00373160

Transferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0017651
Physical Location & Seq
Forward (+)
54427964 .. 54429274
1311 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00373160.1

Sequence Viewer

Length: 1311 bp
ATGGCTACGGTGCAGCTCGAAGTAATATCCAGAGAGCTCATCAAACCCTCCTCTCAAACTCCACATCACCTAAGAAACTACAACCTCTCCTACTTCGATCAGCTCTCTCCTCGTAAGTACATCCCGCTTGTTCTTTTCTATAACCCTTGCAATACCACTACTGCTAGCACTTTTGAGACATCCATTCTCCTCAAAAAATCTTTATCTGAAACCCTAGCCTTTTACTACCCATTTTCCGGAAAGCTCAAAGATCAAGAGTTCATCGACTGCAACGACGAGGGAATTTTGTTCTTAGAAGCAAAGGTCAAACTGAAGTTGTCTGAAATTCTTGAGAATCCGAAACATGAAATTTTGGACCTTTTATTTGCAGACAATTTGCAGTGGAATGATACAAATTTGAGTACTTTAGTTGCTGTACAAGTCAGCTACTTTGAGTGTGGAGGAATGGCATTAAGTGTGTGCATGTCACACAAAATTGGCGATGCAGCAACCATGGTCAATTTCGTCAATGACTGGGCTGCCAAGGCTTCAAACTCAGGTAAACAAGTTTCCCCTTTGCTCTATACACCCTCGATTTTTCCAAGGGGTGACTTACCACTTATGCCGCAGCCGGAATATTCCATCAAGAAAGAAAATTGTGCCTCCAGAAGGTTTGTATTCGATGCCCAAAAAATTGCGGCCCTCAAAGCCATTGTAGCTGACAAAGTGCAAAAGGCTACAAAGGTAGAGGTTGTGAGTGCTCTACTATTTAAGTGTGCAATTTCTGCCGCCAATGCAAGTTCATCAGGCACTACAAGACCAACCCTTTTTCTCCAAAATGTTAACCTTCGTACCAGGATGATTCCTCCGCTACCAGAAAACTATGTGGGTAACATCAGTTGGTTATTTCCAGTACCAAAAAGGGAGGACAGTTTGCATTGCTTCGTGGGTCAAATGAAAGAGGCCTTGTCAGATTTCTGCAACACATATGTTAAGAATGTAAGGGGTACAGAATTGGCCTTGAGCATTAGCAAAACCATGGAGAAGATGCGGACTATGTTCAAAAGCAGTAAGCAATGTGTGCAATACAATTTTAGTAGCTGGTGCAGGTTCCCAGTATACGAAGCAGATTTTGGGTGGGGAAAGCCTGTGTGGGTGAGTATTGGAGCTTGTCTTGACAAGAATGTTATAATTCTGATGGATAGTAAAAGTGGGGATGGGATCGAAGCTTTTGTCACTCTGGAAGAGCAAGACATGGCTGCTTTTGAGTGTGATGAGGAGTTACTAGCATTTGCTGCTCTAAATCCAACTCCATTAACAAGCCAAGTTTGA

Protein Analysis

436

Amino Acids

48.82

Weight (kDa)

6.27

Isoelectric Point (pI)

39.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 5 - 421 4.5e-86 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017651)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24660
malus_domestica MD14G1155800.v1.1
pyrus_communis pycom14g12950
rosa_chinensis RchiOBHm_Chr4g0431761
rosa_laevigata RLG00000006892
rosa_multiflora Rmu_sc0001459.1_g000022
rosa_roxburghii Rroxscaffold_5G00373160
rosa_rugosa Rorug04G0252200
rosa_samantha Rh4AG308600 Rh4BG316400
rosa_wichuraiana Rw4G026770 Rw4G026840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1169
Acc36I ACCTGC 1 cut(s) 1077
AccI GTMKAC 1 cut(s) 1098
AccIII TCCGGA 1 cut(s) 236
AciI CCGC 6 cut(s) 125, 605, 677, 768, 848, 1030
AclWI GGATC 1 cut(s) 1208
AcsI RAATTY 4 cut(s) 282, 324, 348, 394
AcuI CTGAAG 1 cut(s) 332
AfaI GTAC 6 cut(s) 119, 403, 417, 832, 894, 988
AfiI CCNNNNNNNGG 2 cut(s) 236, 648
AgsI TTSAA 2 cut(s) 531, 1042
AjnI CCWGG 1 cut(s) 833
AjuI GAANNNNNNNTTGG 2 cut(s) 1095, 1127
AluBI AGCT 9 cut(s) 16, 37, 103, 244, 426, 698, 1080, 1148, 1208
AluI AGCT 9 cut(s) 16, 37, 103, 244, 426, 698, 1080, 1148, 1208
Alw21I GWGCWC 2 cut(s) 39, 742
Alw26I GTCTC 1 cut(s) 170
AlwI GGATC 1 cut(s) 1208
Aor13HI TCCGGA 1 cut(s) 236
AoxI GGCC 3 cut(s) 678, 942, 996
ApeKI GCWGC 6 cut(s) 13, 485, 518, 607, 1238, 1274
ApoI RAATTY 4 cut(s) 282, 324, 348, 394
ArsI GACNNNNNNTTYG 4 cut(s) 347, 379, 1197, 1229
AspS9I GGNCC 2 cut(s) 355, 679
AsuHPI GGTGA 3 cut(s) 59, 599, 1147
AsuNHI GCTAGC 1 cut(s) 164
AvaII GGWCC 1 cut(s) 355
BaeI ACNNNNGTAYC 2 cut(s) 814, 847
BanII GRGCYC 1 cut(s) 39
Bbv12I GWGCWC 2 cut(s) 39, 742
BbvI GCAGC 6 cut(s) 25, 497, 505, 619, 1225, 1261
BccI CCATC 3 cut(s) 629, 1171, 1190
BciT130I CCWGG 1 cut(s) 835
BcoDI GTCTC 1 cut(s) 170
BfaI CTAG 3 cut(s) 165, 215, 1265
BfuAI ACCTGC 1 cut(s) 1077
BisI GCNGC 9 cut(s) 14, 486, 519, 605, 608, 678, 768, 1239, 1275
BlsI GCNGC 9 cut(s) 15, 487, 520, 606, 609, 679, 769, 1240, 1276
BmcAI AGTACT 1 cut(s) 403
Bme1390I CCNGG 1 cut(s) 835
Bme18I GGWCC 1 cut(s) 355
BmgT120I GGNCC 2 cut(s) 355, 679
BmiI GGNNCC 1 cut(s) 1091
BmrFI CCNGG 1 cut(s) 835
BmrI ACTGGG 2 cut(s) 523, 1088
BmsI GCATC 3 cut(s) 472, 652, 1017
BmtI GCTAGC 1 cut(s) 168
BmuI ACTGGG 2 cut(s) 523, 1088
BpmI CTGGAG 1 cut(s) 628
BpuEI CTTGAG 2 cut(s) 350, 1021
BsaJI CCNNGG 4 cut(s) 492, 522, 581, 1017
BsaWI WCCGGW 1 cut(s) 236
BsaXI ACNNNNNCTCC 2 cut(s) 71, 101
Bsc4I CCNNNNNNNGG 2 cut(s) 236, 648
Bse1I ACTGG 3 cut(s) 518, 890, 1094
Bse3DI GCAATG 2 cut(s) 916, 1061
BseAI TCCGGA 1 cut(s) 236
BseBI CCWGG 1 cut(s) 835
BseDI CCNNGG 4 cut(s) 492, 522, 581, 1017
BseGI GGATG 4 cut(s) 120, 179, 843, 1201
BseLI CCNNNNNNNGG 2 cut(s) 236, 648
BseMI GCAATG 2 cut(s) 916, 1061
BseMII CTCAG 1 cut(s) 549
BseNI ACTGG 3 cut(s) 518, 890, 1094
BseRI GAGGAG 4 cut(s) 40, 99, 179, 1271
BseXI GCAGC 6 cut(s) 25, 497, 505, 619, 1225, 1261
BsgI GTGCAG 2 cut(s) 32, 1105
BshFI GGCC 3 cut(s) 680, 944, 998
BsiHKAI GWGCWC 2 cut(s) 39, 742
BsiSI CCGG 2 cut(s) 237, 611
BslI CCNNNNNNNGG 2 cut(s) 236, 648
BsmAI GTCTC 1 cut(s) 170
BsnI GGCC 3 cut(s) 680, 944, 998
Bsp1286I GDGCHC 2 cut(s) 39, 742
Bsp13I TCCGGA 1 cut(s) 236
Bsp1407I TGTACA 1 cut(s) 415
Bsp143I GATC 3 cut(s) 97, 250, 1200
Bsp19I CCATGG 2 cut(s) 492, 1017
BspACI CCGC 6 cut(s) 125, 605, 677, 768, 848, 1030
BspANI GGCC 3 cut(s) 680, 944, 998
BspCNI CTCAG 1 cut(s) 548
BspEI TCCGGA 1 cut(s) 236
BspLI GGNNCC 1 cut(s) 1091
BspMI ACCTGC 1 cut(s) 1077
BspOI GCTAGC 1 cut(s) 168
BspPI GGATC 1 cut(s) 1208
BspQI GCTCTTC 1 cut(s) 1218
BsrDI GCAATG 2 cut(s) 916, 1061
BsrGI TGTACA 1 cut(s) 415
BsrI ACTGG 3 cut(s) 518, 890, 1094
BssECI CCNNGG 4 cut(s) 492, 522, 581, 1017
BssMI GATC 3 cut(s) 97, 250, 1200
BssNAI GTATAC 1 cut(s) 1099
BssT1I CCWWGG 4 cut(s) 492, 522, 581, 1017
Bst1107I GTATAC 1 cut(s) 1099
Bst2UI CCWGG 1 cut(s) 835
Bst4CI ACNGT 2 cut(s) 10, 911
Bst6I CTCTTC 1 cut(s) 1218
BstAPI GCANNNNNTGC 3 cut(s) 764, 1060, 1274
BstAUI TGTACA 1 cut(s) 415
BstC8I GCNNGC 1 cut(s) 166
BstDEI CTNAG 3 cut(s) 71, 292, 535
BstDSI CCRYGG 2 cut(s) 492, 1017
BstENI CCTNNNNNAGG 1 cut(s) 646
BstF5I GGATG 4 cut(s) 120, 179, 843, 1201
BstKTI GATC 3 cut(s) 100, 253, 1203
BstMAI GTCTC 1 cut(s) 170
BstMBI GATC 3 cut(s) 97, 250, 1200
BstMWI GCNNNNNNNGC 7 cut(s) 524, 686, 695, 764, 773, 1060, 1274
BstNI CCWGG 1 cut(s) 835
BstNSI RCATGY 1 cut(s) 466
BstSCI CCNGG 1 cut(s) 833
BstV1I GCAGC 6 cut(s) 25, 497, 505, 619, 1225, 1261
BstZ17I GTATAC 1 cut(s) 1099
BsuRI GGCC 3 cut(s) 680, 944, 998
BtgI CCRYGG 2 cut(s) 492, 1017
BtgZI GCGATG 1 cut(s) 495
BtsCI GGATG 4 cut(s) 120, 179, 843, 1201
BtsI GCAGTG 1 cut(s) 386
BtsIMutI CAGTG 1 cut(s) 386
BveI ACCTGC 1 cut(s) 1077
Cac8I GCNNGC 1 cut(s) 166
Cfr13I GGNCC 2 cut(s) 355, 679
Csp6I GTAC 6 cut(s) 118, 402, 416, 831, 893, 987
CviAII CATG 5 cut(s) 344, 463, 493, 1018, 1234
CviQI GTAC 6 cut(s) 118, 402, 416, 831, 893, 987
DdeI CTNAG 3 cut(s) 71, 292, 535
DpnI GATC 3 cut(s) 99, 252, 1202
DpnII GATC 3 cut(s) 97, 250, 1200
Eam1104I CTCTTC 1 cut(s) 1218
EarI CTCTTC 1 cut(s) 1218
Ecl136II GAGCTC 1 cut(s) 37
Eco130I CCWWGG 4 cut(s) 492, 522, 581, 1017
Eco147I AGGCCT 1 cut(s) 944
Eco24I GRGCYC 1 cut(s) 39
Eco47I GGWCC 1 cut(s) 355
Eco53kI GAGCTC 1 cut(s) 37
Eco57I CTGAAG 1 cut(s) 332
EcoICRI GAGCTC 1 cut(s) 37
EcoNI CCTNNNNNAGG 1 cut(s) 646
EcoRII CCWGG 1 cut(s) 833
EcoT14I CCWWGG 4 cut(s) 492, 522, 581, 1017
EcoT38I GRGCYC 1 cut(s) 39
ErhI CCWWGG 4 cut(s) 492, 522, 581, 1017
FaeI CATG 5 cut(s) 347, 466, 496, 1021, 1237
FatI CATG 5 cut(s) 343, 462, 492, 1017, 1233
FauI CCCGC 1 cut(s) 132
FauNDI CATATG 1 cut(s) 967
FblI GTMKAC 1 cut(s) 1098
Fnu4HI GCNGC 9 cut(s) 14, 486, 519, 605, 608, 678, 768, 1239, 1275
FokI GGATG 4 cut(s) 107, 166, 850, 1208
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 9 cut(s) 14, 486, 519, 605, 608, 678, 768, 1239, 1275
FspBI CTAG 3 cut(s) 165, 215, 1265
GluI GCNGC 9 cut(s) 14, 486, 519, 605, 608, 678, 768, 1239, 1275
GsuI CTGGAG 1 cut(s) 628
HaeIII GGCC 3 cut(s) 680, 944, 998
HapII CCGG 2 cut(s) 237, 611
Hin1II CATG 5 cut(s) 347, 466, 496, 1021, 1237
HincII GTYRAC 1 cut(s) 823
HindII GTYRAC 1 cut(s) 823
HindIII AAGCTT 1 cut(s) 1206
HinfI GANTC 2 cut(s) 334, 841
HpaI GTTAAC 1 cut(s) 823
HpaII CCGG 2 cut(s) 237, 611
HphI GGTGA 3 cut(s) 59, 599, 1147
Hpy166II GTNNAC 3 cut(s) 542, 823, 1099
Hpy188I TCNGA 5 cut(s) 208, 322, 339, 952, 1176
Hpy188III TCNNGA 8 cut(s) 30, 237, 254, 329, 625, 645, 1154, 1220
Hpy8I GTNNAC 3 cut(s) 542, 823, 1099
Hpy99I CGWCG 1 cut(s) 278
HpyAV CCTTC 2 cut(s) 642, 836
HpyCH4III ACNGT 2 cut(s) 10, 911
HpyF10VI GCNNNNNNNGC 7 cut(s) 524, 686, 695, 764, 773, 1060, 1274
HpyF3I CTNAG 3 cut(s) 71, 292, 535
Hsp92II CATG 5 cut(s) 347, 466, 496, 1021, 1237
Kpn2I TCCGGA 1 cut(s) 236
KspAI GTTAAC 1 cut(s) 823
Kzo9I GATC 3 cut(s) 97, 250, 1200
LguI GCTCTTC 1 cut(s) 1218
LmnI GCTCC 1 cut(s) 1145
Lsp1109I GCAGC 6 cut(s) 25, 497, 505, 619, 1225, 1261
LweI GCATC 3 cut(s) 472, 652, 1017
MaeI CTAG 3 cut(s) 165, 215, 1265
MaeIII GTNAC 5 cut(s) 465, 587, 869, 1213, 1260
MalI GATC 3 cut(s) 99, 252, 1202
MboI GATC 3 cut(s) 97, 250, 1200
MboII GAAGA 2 cut(s) 1036, 1235
MhlI GDGCHC 2 cut(s) 39, 742
MmeI TCCRAC 1 cut(s) 1310
MroI TCCGGA 1 cut(s) 236
MseI TTAA 5 cut(s) 452, 750, 822, 972, 1295
MspI CCGG 2 cut(s) 237, 611
MspR9I CCNGG 1 cut(s) 835
MvaI CCWGG 1 cut(s) 835
MwoI GCNNNNNNNGC 7 cut(s) 524, 686, 695, 764, 773, 1060, 1274
NcoI CCATGG 2 cut(s) 492, 1017
NdeI CATATG 1 cut(s) 967
NdeII GATC 3 cut(s) 97, 250, 1200
NheI GCTAGC 1 cut(s) 164
NlaIII CATG 5 cut(s) 347, 466, 496, 1021, 1237
NlaIV GGNNCC 1 cut(s) 1091
NmuCI GTSAC 3 cut(s) 465, 587, 1213
NspI RCATGY 1 cut(s) 466
PceI AGGCCT 1 cut(s) 944
PciSI GCTCTTC 1 cut(s) 1218
PcsI WCGNNNNNNNCGW 1 cut(s) 270
PfeI GAWTC 2 cut(s) 334, 841
PkrI GCNGC 9 cut(s) 15, 487, 520, 606, 609, 679, 769, 1240, 1276
PsiI TTATAA 1 cut(s) 1169
Psp124BI GAGCTC 1 cut(s) 39
Psp6I CCWGG 1 cut(s) 833
PspGI CCWGG 1 cut(s) 833
PspN4I GGNNCC 1 cut(s) 1091
PspPI GGNCC 2 cut(s) 355, 679
RsaI GTAC 6 cut(s) 119, 403, 417, 832, 894, 988
RsaNI GTAC 6 cut(s) 118, 402, 416, 831, 893, 987
SacI GAGCTC 1 cut(s) 39
SapI GCTCTTC 1 cut(s) 1218
SaqAI TTAA 5 cut(s) 452, 750, 822, 972, 1295
SatI GCNGC 9 cut(s) 14, 486, 519, 605, 608, 678, 768, 1239, 1275
Sau3AI GATC 3 cut(s) 97, 250, 1200
Sau96I GGNCC 2 cut(s) 355, 679
ScaI AGTACT 1 cut(s) 403
ScrFI CCNGG 1 cut(s) 835
SduI GDGCHC 2 cut(s) 39, 742
SfaNI GCATC 3 cut(s) 472, 652, 1017
SinI GGWCC 1 cut(s) 355
SmlI CTYRAG 2 cut(s) 329, 1000
SmoI CTYRAG 2 cut(s) 329, 1000
SseBI AGGCCT 1 cut(s) 944
SsiI CCGC 6 cut(s) 125, 605, 677, 768, 848, 1030
SspI AATATT 1 cut(s) 617
SspMI CTAG 3 cut(s) 165, 215, 1265
SstI GAGCTC 1 cut(s) 39
StuI AGGCCT 1 cut(s) 944
StyD4I CCNGG 1 cut(s) 833
StyI CCWWGG 4 cut(s) 492, 522, 581, 1017
TaaI ACNGT 2 cut(s) 10, 911
TaqI TCGA 6 cut(s) 18, 96, 264, 572, 660, 1203
TatI WGTACW 3 cut(s) 117, 401, 415
TauI GCSGC 3 cut(s) 607, 680, 770
TfiI GAWTC 2 cut(s) 334, 841
Tru1I TTAA 5 cut(s) 452, 750, 822, 972, 1295
Tru9I TTAA 5 cut(s) 452, 750, 822, 972, 1295
TscAI CASTG 1 cut(s) 386
TseFI GTSAC 3 cut(s) 465, 587, 1213
TseI GCWGC 6 cut(s) 13, 485, 518, 607, 1238, 1274
Tsp45I GTSAC 3 cut(s) 465, 587, 1213
TspDTI ATGAA 4 cut(s) 250, 360, 771, 950
TspRI CASTG 1 cut(s) 386
VpaK11BI GGWCC 1 cut(s) 355
XagI CCTNNNNNAGG 1 cut(s) 646
XapI RAATTY 4 cut(s) 282, 324, 348, 394
XceI RCATGY 1 cut(s) 466
XmiI GTMKAC 1 cut(s) 1098
XspI CTAG 3 cut(s) 165, 215, 1265
ZrmI AGTACT 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.