Rw4G026840

Transferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0017651
Physical Location & Seq
Forward (+)
53103619 .. 53104917
1299 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G026840.1

Sequence Viewer

Length: 1299 bp
ATGGCTACGGTGCAGCTGGAAGTAATATCCAGAGAGCTCATCAAACCCTCCTCTCAAACTCCACATCACCTAAGAAACTACGACCTCTCCTACTTCGATCAGCTCTCTCCTCGTAAGTACATCCCGCTTATTCTTTTCTATAACCCTTGCAATACCACTTTTGAGACATCCATTCTCCTCAAAAAATCTTTATCTGAAACCCTAGCCTTTTACTACCCATTTGCCGGAAAGGTCAAAGATCAAGAGTTCATCGACTGCAACGACGAGGGAATTTTGTTCTTAGAAGCAAAGGTCAAACTGAAGTTGTCTGAAATTCTTGAGAATCCGAAACATGAAATTTTGGACCTTTTATTTGCAGACAACTTGCAGTGGAATGATACGAATTTGAGTACTTTACTTGCTGTACAAGTCAGCTACTTTGATTGTGGGGGAATGGCATTAAGTGTGTGCATGTCACACAAAATTGGCGATGCAGCAACCATGGTCAATTTCATCAATGACTGGGCCGCCAAGGCTTCAAACTCAGGTAAACAAGTTTCCCCTTTGCTCTATACACCCTCGATTTTTCCAAGGGGTGACTTACCACTTATGCCGCAGCCGGAATATTCCATCAAGAAAGAAAATTGTGCCTCCAGAAGGTTTGTATTCGATGCCCAAAAAATTGCGGTCCTCAAAGCCATTGTAGCTGACAAAGTGCAAAAGGCTACAAAGGTAGAGGTTGTGAGTGCTCTACTATTTAAGTGTGCAATTTCTGCCGCCAATGCAAGTTCATCAGGTACTACAAGACCAATCCTTTTTCTCCAAAATGTTAACCTTCGTACCAGGATGATTCCTCCGCTACCAGAAAACTATGTGGGTAACATCAGTTGGGTATTTCCAGTACCAAAAAGGGAGGACAGTTTGCATTGCTTGGTGGGTCAAATGAAAGAGGCCTTGTCAGATTTCTGCAACACATATGTTAAGAATGTAAGGGGTACAGAATTGGCCTTGAGCATTAGCAAAACCATGGAGAAGATGCGGGCTATGTTCAAAAGCAGTAAGCAATGTGTGCAATACAATTGTAGTAGCTGGTGCAGGTTCCCAGTATACGAAGCAGATTTTGGGTGGGGAAAGCCTGTGTGGGTGAGTATTGGGGCTTGTCTTGACAAGAATGTTATAATTCTGATGGATAGTAAAAGTGGGGATGGGATCGAAGCTTTTGTGACTCTGGAAGAGCAAGACATGGCTGCTTTTGAGTGTGATGAGGAGTTACTAGCATTTGCTGCTCTAAATCCAACTCCATTAACAAGTCAAGTTTGA

Protein Analysis

432

Amino Acids

48.38

Weight (kDa)

6.05

Isoelectric Point (pI)

41.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 5 - 417 1.8e-85 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017651)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24660
malus_domestica MD14G1155800.v1.1
pyrus_communis pycom14g12950
rosa_chinensis RchiOBHm_Chr4g0431761
rosa_laevigata RLG00000006892
rosa_multiflora Rmu_sc0001459.1_g000022
rosa_roxburghii Rroxscaffold_5G00373160
rosa_rugosa Rorug04G0252200
rosa_samantha Rh4AG308600 Rh4BG316400
rosa_wichuraiana Rw4G026770 Rw4G026840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1157
Acc36I ACCTGC 1 cut(s) 1065
AccI GTMKAC 1 cut(s) 1086
AciI CCGC 7 cut(s) 125, 507, 593, 665, 756, 836, 1018
AclWI GGATC 1 cut(s) 1196
AcsI RAATTY 4 cut(s) 270, 312, 336, 382
AcuI CTGAAG 1 cut(s) 320
AfaI GTAC 7 cut(s) 119, 391, 405, 778, 820, 882, 976
AfiI CCNNNNNNNGG 2 cut(s) 224, 636
AgsI TTSAA 2 cut(s) 519, 1030
AjnI CCWGG 1 cut(s) 821
AjuI GAANNNNNNNTTGG 2 cut(s) 1083, 1115
AluBI AGCT 7 cut(s) 16, 37, 103, 414, 686, 1068, 1196
AluI AGCT 7 cut(s) 16, 37, 103, 414, 686, 1068, 1196
Alw21I GWGCWC 2 cut(s) 39, 730
Alw26I GTCTC 1 cut(s) 158
AlwI GGATC 1 cut(s) 1196
AoxI GGCC 3 cut(s) 504, 930, 984
ApeKI GCWGC 5 cut(s) 13, 473, 595, 1226, 1262
ApoI RAATTY 4 cut(s) 270, 312, 336, 382
ArsI GACNNNNNNTTYG 2 cut(s) 335, 367
AspS9I GGNCC 3 cut(s) 343, 504, 667
AsuHPI GGTGA 3 cut(s) 59, 587, 1135
AvaII GGWCC 2 cut(s) 343, 667
BaeI ACNNNNGTAYC 2 cut(s) 802, 835
BanII GRGCYC 1 cut(s) 39
Bbv12I GWGCWC 2 cut(s) 39, 730
BbvI GCAGC 5 cut(s) 25, 485, 607, 1213, 1249
BccI CCATC 3 cut(s) 617, 1159, 1178
BciT130I CCWGG 1 cut(s) 823
BcoDI GTCTC 1 cut(s) 158
BfaI CTAG 2 cut(s) 203, 1253
BfuAI ACCTGC 1 cut(s) 1065
BglI GCCNNNNNGGC 1 cut(s) 512
BisI GCNGC 8 cut(s) 14, 474, 507, 593, 596, 756, 1227, 1263
BlsI GCNGC 8 cut(s) 15, 475, 508, 594, 597, 757, 1228, 1264
BmcAI AGTACT 1 cut(s) 391
Bme1390I CCNGG 1 cut(s) 823
Bme18I GGWCC 2 cut(s) 343, 667
BmgT120I GGNCC 3 cut(s) 343, 504, 667
BmiI GGNNCC 1 cut(s) 1079
BmrFI CCNGG 1 cut(s) 823
BmrI ACTGGG 2 cut(s) 511, 1076
BmsI GCATC 3 cut(s) 460, 640, 1005
BmuI ACTGGG 2 cut(s) 511, 1076
BpmI CTGGAG 1 cut(s) 616
BpuEI CTTGAG 2 cut(s) 338, 1009
BsaJI CCNNGG 4 cut(s) 480, 510, 569, 1005
BsaXI ACNNNNNCTCC 2 cut(s) 71, 101
Bsc4I CCNNNNNNNGG 2 cut(s) 224, 636
Bse1I ACTGG 3 cut(s) 506, 878, 1082
Bse3DI GCAATG 2 cut(s) 904, 1049
BseBI CCWGG 1 cut(s) 823
BseDI CCNNGG 4 cut(s) 480, 510, 569, 1005
BseGI GGATG 4 cut(s) 120, 167, 831, 1189
BseLI CCNNNNNNNGG 2 cut(s) 224, 636
BseMI GCAATG 2 cut(s) 904, 1049
BseMII CTCAG 1 cut(s) 537
BseNI ACTGG 3 cut(s) 506, 878, 1082
BseRI GAGGAG 4 cut(s) 40, 99, 167, 1259
BseXI GCAGC 5 cut(s) 25, 485, 607, 1213, 1249
BsgI GTGCAG 2 cut(s) 32, 1093
BshFI GGCC 3 cut(s) 506, 932, 986
BsiHKAI GWGCWC 2 cut(s) 39, 730
BsiSI CCGG 2 cut(s) 225, 599
BslI CCNNNNNNNGG 2 cut(s) 224, 636
BsmAI GTCTC 1 cut(s) 158
BsnI GGCC 3 cut(s) 506, 932, 986
Bsp1286I GDGCHC 2 cut(s) 39, 730
Bsp1407I TGTACA 1 cut(s) 403
Bsp143I GATC 3 cut(s) 97, 238, 1188
Bsp19I CCATGG 2 cut(s) 480, 1005
BspACI CCGC 7 cut(s) 125, 507, 593, 665, 756, 836, 1018
BspANI GGCC 3 cut(s) 506, 932, 986
BspCNI CTCAG 1 cut(s) 536
BspLI GGNNCC 1 cut(s) 1079
BspMI ACCTGC 1 cut(s) 1065
BspPI GGATC 1 cut(s) 1196
BspQI GCTCTTC 1 cut(s) 1206
BsrDI GCAATG 2 cut(s) 904, 1049
BsrGI TGTACA 1 cut(s) 403
BsrI ACTGG 3 cut(s) 506, 878, 1082
BssECI CCNNGG 4 cut(s) 480, 510, 569, 1005
BssMI GATC 3 cut(s) 97, 238, 1188
BssNAI GTATAC 1 cut(s) 1087
BssT1I CCWWGG 4 cut(s) 480, 510, 569, 1005
Bst1107I GTATAC 1 cut(s) 1087
Bst2UI CCWGG 1 cut(s) 823
Bst4CI ACNGT 2 cut(s) 10, 899
Bst6I CTCTTC 1 cut(s) 1206
BstAPI GCANNNNNTGC 3 cut(s) 752, 1048, 1262
BstAUI TGTACA 1 cut(s) 403
BstC8I GCNNGC 1 cut(s) 1020
BstDEI CTNAG 3 cut(s) 71, 280, 523
BstDSI CCRYGG 2 cut(s) 480, 1005
BstENI CCTNNNNNAGG 1 cut(s) 634
BstF5I GGATG 4 cut(s) 120, 167, 831, 1189
BstKTI GATC 3 cut(s) 100, 241, 1191
BstMAI GTCTC 1 cut(s) 158
BstMBI GATC 3 cut(s) 97, 238, 1188
BstMWI GCNNNNNNNGC 6 cut(s) 512, 683, 752, 761, 1048, 1262
BstNI CCWGG 1 cut(s) 823
BstNSI RCATGY 1 cut(s) 454
BstSCI CCNGG 1 cut(s) 821
BstV1I GCAGC 5 cut(s) 25, 485, 607, 1213, 1249
BstZ17I GTATAC 1 cut(s) 1087
BsuRI GGCC 3 cut(s) 506, 932, 986
BtgI CCRYGG 2 cut(s) 480, 1005
BtgZI GCGATG 1 cut(s) 483
BtsCI GGATG 4 cut(s) 120, 167, 831, 1189
BtsI GCAGTG 1 cut(s) 374
BtsIMutI CAGTG 1 cut(s) 374
BveI ACCTGC 1 cut(s) 1065
Cac8I GCNNGC 1 cut(s) 1020
Cfr13I GGNCC 3 cut(s) 343, 504, 667
Csp6I GTAC 7 cut(s) 118, 390, 404, 777, 819, 881, 975
CviAII CATG 5 cut(s) 332, 451, 481, 1006, 1222
CviQI GTAC 7 cut(s) 118, 390, 404, 777, 819, 881, 975
DdeI CTNAG 3 cut(s) 71, 280, 523
DpnI GATC 3 cut(s) 99, 240, 1190
DpnII GATC 3 cut(s) 97, 238, 1188
Eam1104I CTCTTC 1 cut(s) 1206
EarI CTCTTC 1 cut(s) 1206
Ecl136II GAGCTC 1 cut(s) 37
Eco130I CCWWGG 4 cut(s) 480, 510, 569, 1005
Eco147I AGGCCT 1 cut(s) 932
Eco24I GRGCYC 1 cut(s) 39
Eco47I GGWCC 2 cut(s) 343, 667
Eco53kI GAGCTC 1 cut(s) 37
Eco57I CTGAAG 1 cut(s) 320
EcoICRI GAGCTC 1 cut(s) 37
EcoNI CCTNNNNNAGG 1 cut(s) 634
EcoRII CCWGG 1 cut(s) 821
EcoT14I CCWWGG 4 cut(s) 480, 510, 569, 1005
EcoT38I GRGCYC 1 cut(s) 39
ErhI CCWWGG 4 cut(s) 480, 510, 569, 1005
FaeI CATG 5 cut(s) 335, 454, 484, 1009, 1225
FatI CATG 5 cut(s) 331, 450, 480, 1005, 1221
FauI CCCGC 2 cut(s) 132, 1011
FauNDI CATATG 1 cut(s) 955
FblI GTMKAC 1 cut(s) 1086
Fnu4HI GCNGC 8 cut(s) 14, 474, 507, 593, 596, 756, 1227, 1263
FokI GGATG 4 cut(s) 107, 154, 838, 1196
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 8 cut(s) 14, 474, 507, 593, 596, 756, 1227, 1263
FspBI CTAG 2 cut(s) 203, 1253
GluI GCNGC 8 cut(s) 14, 474, 507, 593, 596, 756, 1227, 1263
GsuI CTGGAG 1 cut(s) 616
HaeIII GGCC 3 cut(s) 506, 932, 986
HapII CCGG 2 cut(s) 225, 599
Hin1II CATG 5 cut(s) 335, 454, 484, 1009, 1225
HincII GTYRAC 1 cut(s) 811
HindII GTYRAC 1 cut(s) 811
HindIII AAGCTT 1 cut(s) 1194
HinfI GANTC 3 cut(s) 322, 829, 1204
HpaI GTTAAC 1 cut(s) 811
HpaII CCGG 2 cut(s) 225, 599
HphI GGTGA 3 cut(s) 59, 587, 1135
Hpy166II GTNNAC 3 cut(s) 530, 811, 1087
Hpy188I TCNGA 5 cut(s) 196, 310, 327, 940, 1164
Hpy188III TCNNGA 7 cut(s) 30, 242, 317, 613, 633, 1142, 1208
Hpy8I GTNNAC 3 cut(s) 530, 811, 1087
Hpy99I CGWCG 1 cut(s) 266
HpyAV CCTTC 2 cut(s) 630, 824
HpyCH4III ACNGT 2 cut(s) 10, 899
HpyF10VI GCNNNNNNNGC 6 cut(s) 512, 683, 752, 761, 1048, 1262
HpyF3I CTNAG 3 cut(s) 71, 280, 523
Hsp92II CATG 5 cut(s) 335, 454, 484, 1009, 1225
KspAI GTTAAC 1 cut(s) 811
Kzo9I GATC 3 cut(s) 97, 238, 1188
LguI GCTCTTC 1 cut(s) 1206
Lsp1109I GCAGC 5 cut(s) 25, 485, 607, 1213, 1249
LweI GCATC 3 cut(s) 460, 640, 1005
MaeI CTAG 2 cut(s) 203, 1253
MaeIII GTNAC 5 cut(s) 453, 575, 857, 1201, 1248
MalI GATC 3 cut(s) 99, 240, 1190
MboI GATC 3 cut(s) 97, 238, 1188
MboII GAAGA 2 cut(s) 1024, 1223
MfeI CAATTG 1 cut(s) 1057
MhlI GDGCHC 2 cut(s) 39, 730
MlyI GAGTC 1 cut(s) 1198
MmeI TCCRAC 1 cut(s) 1298
MseI TTAA 5 cut(s) 440, 738, 810, 960, 1283
MspA1I CMGCKG 1 cut(s) 16
MspI CCGG 2 cut(s) 225, 599
MspR9I CCNGG 1 cut(s) 823
MunI CAATTG 1 cut(s) 1057
MvaI CCWGG 1 cut(s) 823
MwoI GCNNNNNNNGC 6 cut(s) 512, 683, 752, 761, 1048, 1262
NcoI CCATGG 2 cut(s) 480, 1005
NdeI CATATG 1 cut(s) 955
NdeII GATC 3 cut(s) 97, 238, 1188
NlaIII CATG 5 cut(s) 335, 454, 484, 1009, 1225
NlaIV GGNNCC 1 cut(s) 1079
NmuCI GTSAC 3 cut(s) 453, 575, 1201
NspI RCATGY 1 cut(s) 454
PceI AGGCCT 1 cut(s) 932
PciSI GCTCTTC 1 cut(s) 1206
PcsI WCGNNNNNNNCGW 1 cut(s) 258
PfeI GAWTC 2 cut(s) 322, 829
PkrI GCNGC 8 cut(s) 15, 475, 508, 594, 597, 757, 1228, 1264
PleI GAGTC 1 cut(s) 1198
PpsI GAGTC 1 cut(s) 1198
PsiI TTATAA 1 cut(s) 1157
Psp124BI GAGCTC 1 cut(s) 39
Psp6I CCWGG 1 cut(s) 821
PspGI CCWGG 1 cut(s) 821
PspN4I GGNNCC 1 cut(s) 1079
PspPI GGNCC 3 cut(s) 343, 504, 667
PvuII CAGCTG 1 cut(s) 16
RsaI GTAC 7 cut(s) 119, 391, 405, 778, 820, 882, 976
RsaNI GTAC 7 cut(s) 118, 390, 404, 777, 819, 881, 975
SacI GAGCTC 1 cut(s) 39
SapI GCTCTTC 1 cut(s) 1206
SaqAI TTAA 5 cut(s) 440, 738, 810, 960, 1283
SatI GCNGC 8 cut(s) 14, 474, 507, 593, 596, 756, 1227, 1263
Sau3AI GATC 3 cut(s) 97, 238, 1188
Sau96I GGNCC 3 cut(s) 343, 504, 667
ScaI AGTACT 1 cut(s) 391
SchI GAGTC 1 cut(s) 1198
ScrFI CCNGG 1 cut(s) 823
SduI GDGCHC 2 cut(s) 39, 730
SfaNI GCATC 3 cut(s) 460, 640, 1005
SinI GGWCC 2 cut(s) 343, 667
SmlI CTYRAG 2 cut(s) 317, 988
SmoI CTYRAG 2 cut(s) 317, 988
SseBI AGGCCT 1 cut(s) 932
SsiI CCGC 7 cut(s) 125, 507, 593, 665, 756, 836, 1018
SspI AATATT 1 cut(s) 605
SspMI CTAG 2 cut(s) 203, 1253
SstI GAGCTC 1 cut(s) 39
StuI AGGCCT 1 cut(s) 932
StyD4I CCNGG 1 cut(s) 821
StyI CCWWGG 4 cut(s) 480, 510, 569, 1005
TaaI ACNGT 2 cut(s) 10, 899
TaqI TCGA 5 cut(s) 96, 252, 560, 648, 1191
TatI WGTACW 3 cut(s) 117, 389, 403
TauI GCSGC 3 cut(s) 509, 595, 758
TfiI GAWTC 2 cut(s) 322, 829
Tru1I TTAA 5 cut(s) 440, 738, 810, 960, 1283
Tru9I TTAA 5 cut(s) 440, 738, 810, 960, 1283
TscAI CASTG 1 cut(s) 374
TseFI GTSAC 3 cut(s) 453, 575, 1201
TseI GCWGC 5 cut(s) 13, 473, 595, 1226, 1262
Tsp45I GTSAC 3 cut(s) 453, 575, 1201
TspDTI ATGAA 5 cut(s) 238, 348, 481, 759, 938
TspRI CASTG 1 cut(s) 374
VpaK11BI GGWCC 2 cut(s) 343, 667
XagI CCTNNNNNAGG 1 cut(s) 634
XapI RAATTY 4 cut(s) 270, 312, 336, 382
XceI RCATGY 1 cut(s) 454
XmiI GTMKAC 1 cut(s) 1086
XspI CTAG 2 cut(s) 203, 1253
ZrmI AGTACT 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.