MD14G1156900.v1.1

Deoxycytidylate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0009957
Physical Location & Seq
Forward (+)
25108341 .. 25111448
3108 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1156900.v1.1.491

Sequence Viewer

Length: 678 bp
ATGAACTCCCGCGAGCTCGCCCTGGTCTCCACCGCCGCCGTTTTCGGTGCCCTGGCCTCTGCGCTCACTAGCCGTTTCTTCTTTTCAAACCCTAAGAGGCAGTCCCCCAAACTCGATTTGTCGAAAAACGGCGTCGTTTCCAGGAAATGCTCTTCTCAGAGCCCTTTCGACCCCTCAAAGCGCGAAGGATATTTATCATGGGATGATTATTTCATGGCAATTGCATTTTTATCAGCTGAAAGATCCAAAGATCCTAACAGGCAGGTTGGAGCATGCTTGGTGAGTGAAAATGGTGTAATTCTAGGAATTGGATATAATGGGTTCCCGAGAGGTTGTTCGGATGACAAGCTTCCTTGGGCAAAGAAATCCAGAACTGGGGATCCTTTGGAGACAAAGTATCCTTATGTTTGTCATGCTGAAGTCAATGCTATCCTAAACACGAATCATGCTTCTGCTGCAGGACAGAGGCTTTATGTGACCATGTTCCCCTGCAATGAGTGTGCCAAGATAATCATTCAGTCAGGAGTCGCCGAAGTTATATATTTTGTGGAAAAGAGGTTAAACAATCCAGAAGTTGCCTACATTGCTTCCCATAAACTACTATCATTGGCTGGTGTGAAAGTCAGGAAACATCAACCATACCTGAACCAAATTTTAGTCAAGTTTGAGGAGCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00240 ko01100 map00240 map01100
Pfam Domains
Protein Families
dCMP_cyt_deam_1

Protein Analysis

226

Amino Acids

24.91

Weight (kDa)

9.19

Isoelectric Point (pI)

42.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
dCMP_cyt_deam_1 PF00383 66 - 182 1.6e-24 Cytidine and deoxycytidylate deaminase zinc-binding region
MafB19-deam PF14437 66 - 186 7.9e-13 MafB19-like deaminase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 253
AccB1I GGYRCC 1 cut(s) 47
AccII CGCG 2 cut(s) 12, 183
AciI CCGC 3 cut(s) 10, 33, 36
AclWI GGATC 4 cut(s) 237, 245, 374, 387
AcsI RAATTY 1 cut(s) 651
AcuI CTGAAG 1 cut(s) 438
AcyI GRCGYC 1 cut(s) 132
AfiI CCNNNNNNNGG 1 cut(s) 375
AgsI TTSAA 1 cut(s) 87
AjnI CCWGG 3 cut(s) 21, 51, 140
AloI GAACNNNNNNTCC 2 cut(s) 364, 396
AluBI AGCT 3 cut(s) 16, 236, 349
AluI AGCT 3 cut(s) 16, 236, 349
Alw21I GWGCWC 1 cut(s) 18
Alw26I GTCTC 2 cut(s) 31, 383
AlwI GGATC 4 cut(s) 237, 245, 374, 387
Ama87I CYCGRG 1 cut(s) 325
AoxI GGCC 1 cut(s) 54
ApeKI GCWGC 1 cut(s) 455
ApoI RAATTY 1 cut(s) 651
AspLEI GCGC 2 cut(s) 64, 183
AsuHPI GGTGA 1 cut(s) 292
AvaI CYCGRG 1 cut(s) 325
BaeGI GKGCMC 1 cut(s) 52
BamHI GGATCC 1 cut(s) 379
BanI GGYRCC 1 cut(s) 47
BanII GRGCYC 3 cut(s) 18, 164, 675
Bbv12I GWGCWC 1 cut(s) 18
BbvI GCAGC 1 cut(s) 442
BceAI ACGGC 3 cut(s) 23, 57, 145
BciT130I CCWGG 3 cut(s) 23, 53, 142
BciVI GTATCC 1 cut(s) 408
BcoDI GTCTC 2 cut(s) 31, 383
BfaI CTAG 3 cut(s) 69, 302, 676
BfmI CTRYAG 1 cut(s) 456
BfuAI ACCTGC 1 cut(s) 253
BfuI GTATCC 1 cut(s) 408
BisI GCNGC 2 cut(s) 36, 456
BlsI GCNGC 2 cut(s) 37, 457
Bme1390I CCNGG 3 cut(s) 23, 53, 142
BmeT110I CYCGRG 1 cut(s) 325
BmiI GGNNCC 4 cut(s) 49, 323, 381, 672
BmrFI CCNGG 3 cut(s) 23, 53, 142
BmrI ACTGGG 1 cut(s) 384
BmuI ACTGGG 1 cut(s) 384
BsaBI GATNNNNATC 1 cut(s) 193
BsaHI GRCGYC 1 cut(s) 132
BsaI GGTCTC 1 cut(s) 31
BsaJI CCNNGG 3 cut(s) 21, 51, 353
BsaXI ACNNNNNCTCC 2 cut(s) 380, 410
Bsc4I CCNNNNNNNGG 1 cut(s) 375
Bse1I ACTGG 1 cut(s) 379
Bse3DI GCAATG 2 cut(s) 499, 582
Bse8I GATNNNNATC 1 cut(s) 193
BseBI CCWGG 3 cut(s) 23, 53, 142
BseDI CCNNGG 3 cut(s) 21, 51, 353
BseGI GGATG 2 cut(s) 208, 346
BseJI GATNNNNATC 1 cut(s) 193
BseLI CCNNNNNNNGG 1 cut(s) 375
BseMI GCAATG 2 cut(s) 499, 582
BseMII CTCAG 1 cut(s) 170
BseNI ACTGG 1 cut(s) 379
BseSI GKGCMC 1 cut(s) 52
BseXI GCAGC 1 cut(s) 442
Bsh1236I CGCG 2 cut(s) 12, 183
BshFI GGCC 1 cut(s) 56
BshNI GGYRCC 1 cut(s) 47
BsiHKAI GWGCWC 1 cut(s) 18
BsiHKCI CYCGRG 1 cut(s) 325
BslFI GGGAC 1 cut(s) 88
BslI CCNNNNNNNGG 1 cut(s) 375
BsmAI GTCTC 2 cut(s) 31, 383
BsmFI GGGAC 1 cut(s) 88
BsnI GGCC 1 cut(s) 56
Bso31I GGTCTC 1 cut(s) 31
BsoBI CYCGRG 1 cut(s) 325
Bsp1286I GDGCHC 4 cut(s) 18, 52, 164, 675
Bsp143I GATC 3 cut(s) 242, 250, 379
BspACI CCGC 3 cut(s) 10, 33, 36
BspANI GGCC 1 cut(s) 56
BspCNI CTCAG 1 cut(s) 169
BspFNI CGCG 2 cut(s) 12, 183
BspLI GGNNCC 4 cut(s) 49, 323, 381, 672
BspMAI CTGCAG 1 cut(s) 460
BspMI ACCTGC 1 cut(s) 253
BspPI GGATC 4 cut(s) 237, 245, 374, 387
BspQI GCTCTTC 1 cut(s) 157
BspT107I GGYRCC 1 cut(s) 47
BspTNI GGTCTC 1 cut(s) 31
BsrDI GCAATG 2 cut(s) 499, 582
BsrI ACTGG 1 cut(s) 379
BssECI CCNNGG 3 cut(s) 21, 51, 353
BssMI GATC 3 cut(s) 242, 250, 379
BssNI GRCGYC 1 cut(s) 132
BssT1I CCWWGG 1 cut(s) 353
Bst2UI CCWGG 3 cut(s) 23, 53, 142
Bst6I CTCTTC 1 cut(s) 157
BstACI GRCGYC 1 cut(s) 132
BstC8I GCNNGC 3 cut(s) 14, 18, 274
BstDEI CTNAG 2 cut(s) 93, 156
BstF5I GGATG 2 cut(s) 208, 346
BstFNI CGCG 2 cut(s) 12, 183
BstHHI GCGC 2 cut(s) 64, 183
BstKTI GATC 3 cut(s) 245, 253, 382
BstMAI GTCTC 2 cut(s) 31, 383
BstMBI GATC 3 cut(s) 242, 250, 379
BstMWI GCNNNNNNNGC 2 cut(s) 455, 584
BstNI CCWGG 3 cut(s) 23, 53, 142
BstNSI RCATGY 1 cut(s) 276
BstSCI CCNGG 3 cut(s) 21, 51, 140
BstSFI CTRYAG 1 cut(s) 456
BstSLI GKGCMC 1 cut(s) 52
BstUI CGCG 2 cut(s) 12, 183
BstV1I GCAGC 1 cut(s) 442
BstX2I RGATCY 3 cut(s) 242, 250, 379
BstYI RGATCY 3 cut(s) 242, 250, 379
BsuI GTATCC 1 cut(s) 408
BsuRI GGCC 1 cut(s) 56
BtsCI GGATG 2 cut(s) 208, 346
BveI ACCTGC 1 cut(s) 253
Cac8I GCNNGC 3 cut(s) 14, 18, 274
CfoI GCGC 2 cut(s) 64, 183
CseI GACGC 1 cut(s) 121
CviAII CATG 6 cut(s) 198, 214, 273, 413, 446, 481
CviJI RGCY 9 cut(s) 16, 56, 72, 162, 236, 349, 469, 611, 673
CviKI_1 RGCY 9 cut(s) 16, 56, 72, 162, 236, 349, 469, 611, 673
DdeI CTNAG 2 cut(s) 93, 156
DpnI GATC 3 cut(s) 244, 252, 381
DpnII GATC 3 cut(s) 242, 250, 379
Eam1104I CTCTTC 1 cut(s) 157
EarI CTCTTC 1 cut(s) 157
Ecl136II GAGCTC 1 cut(s) 16
Eco130I CCWWGG 1 cut(s) 353
Eco24I GRGCYC 3 cut(s) 18, 164, 675
Eco31I GGTCTC 1 cut(s) 31
Eco53kI GAGCTC 1 cut(s) 16
Eco57I CTGAAG 1 cut(s) 438
Eco88I CYCGRG 1 cut(s) 325
EcoICRI GAGCTC 1 cut(s) 16
EcoRII CCWGG 3 cut(s) 21, 51, 140
EcoT14I CCWWGG 1 cut(s) 353
EcoT38I GRGCYC 3 cut(s) 18, 164, 675
ErhI CCWWGG 1 cut(s) 353
FaeI CATG 6 cut(s) 201, 217, 276, 416, 449, 484
FaqI GGGAC 1 cut(s) 88
FatI CATG 6 cut(s) 197, 213, 272, 412, 445, 480
FauI CCCGC 1 cut(s) 17
Fnu4HI GCNGC 2 cut(s) 36, 456
FokI GGATG 2 cut(s) 215, 353
FriOI GRGCYC 3 cut(s) 18, 164, 675
Fsp4HI GCNGC 2 cut(s) 36, 456
FspBI CTAG 3 cut(s) 69, 302, 676
GlaI GCGC 2 cut(s) 63, 182
GluI GCNGC 2 cut(s) 36, 456
HaeIII GGCC 1 cut(s) 56
HgaI GACGC 1 cut(s) 121
HhaI GCGC 2 cut(s) 64, 183
Hin1I GRCGYC 1 cut(s) 132
Hin1II CATG 6 cut(s) 201, 217, 276, 416, 449, 484
Hin6I GCGC 2 cut(s) 62, 181
HinP1I GCGC 2 cut(s) 62, 181
HindIII AAGCTT 1 cut(s) 347
HinfI GANTC 2 cut(s) 442, 525
HphI GGTGA 1 cut(s) 292
Hpy188I TCNGA 2 cut(s) 159, 340
Hpy188III TCNNGA 5 cut(s) 325, 369, 522, 569, 625
Hpy99I CGWCG 1 cut(s) 137
HpyAV CCTTC 1 cut(s) 179
HpyCH4V TGCA 3 cut(s) 224, 458, 492
HpyF10VI GCNNNNNNNGC 2 cut(s) 455, 584
HpyF3I CTNAG 2 cut(s) 93, 156
Hsp92I GRCGYC 1 cut(s) 132
Hsp92II CATG 6 cut(s) 201, 217, 276, 416, 449, 484
HspAI GCGC 2 cut(s) 62, 181
Kzo9I GATC 3 cut(s) 242, 250, 379
LguI GCTCTTC 1 cut(s) 157
LmnI GCTCC 2 cut(s) 269, 670
Lsp1109I GCAGC 1 cut(s) 442
MaeI CTAG 3 cut(s) 69, 302, 676
MaeIII GTNAC 1 cut(s) 475
MalI GATC 3 cut(s) 244, 252, 381
MboI GATC 3 cut(s) 242, 250, 379
MboII GAAGA 2 cut(s) 70, 144
MfeI CAATTG 1 cut(s) 219
MflI RGATCY 3 cut(s) 242, 250, 379
MhlI GDGCHC 4 cut(s) 18, 52, 164, 675
MluCI AATT 4 cut(s) 219, 297, 306, 651
MlyI GAGTC 1 cut(s) 534
MmeI TCCRAC 1 cut(s) 247
MnlI CCTC 7 cut(s) 67, 90, 184, 323, 459, 549, 661
MseI TTAA 1 cut(s) 560
MspA1I CMGCKG 1 cut(s) 236
MspR9I CCNGG 3 cut(s) 23, 53, 142
MunI CAATTG 1 cut(s) 219
MvaI CCWGG 3 cut(s) 23, 53, 142
MvnI CGCG 2 cut(s) 12, 183
MwoI GCNNNNNNNGC 2 cut(s) 455, 584
NdeII GATC 3 cut(s) 242, 250, 379
NlaIII CATG 6 cut(s) 201, 217, 276, 416, 449, 484
NlaIV GGNNCC 4 cut(s) 49, 323, 381, 672
NmuCI GTSAC 1 cut(s) 475
NspI RCATGY 1 cut(s) 276
PaeI GCATGC 1 cut(s) 276
PciSI GCTCTTC 1 cut(s) 157
PfeI GAWTC 1 cut(s) 442
PfoI TCCNGGA 1 cut(s) 140
PkrI GCNGC 2 cut(s) 37, 457
PleI GAGTC 1 cut(s) 533
PpsI GAGTC 1 cut(s) 533
Psp124BI GAGCTC 1 cut(s) 18
Psp6I CCWGG 3 cut(s) 21, 51, 140
PspGI CCWGG 3 cut(s) 21, 51, 140
PspN4I GGNNCC 4 cut(s) 49, 323, 381, 672
PstI CTGCAG 1 cut(s) 460
PsuI RGATCY 3 cut(s) 242, 250, 379
PvuII CAGCTG 1 cut(s) 236
SacI GAGCTC 1 cut(s) 18
SapI GCTCTTC 1 cut(s) 157
SaqAI TTAA 1 cut(s) 560
SatI GCNGC 2 cut(s) 36, 456
Sau3AI GATC 3 cut(s) 242, 250, 379
SchI GAGTC 1 cut(s) 534
ScrFI CCNGG 3 cut(s) 23, 53, 142
SduI GDGCHC 4 cut(s) 18, 52, 164, 675
SetI ASST 7 cut(s) 18, 238, 267, 334, 351, 560, 645
SfcI CTRYAG 1 cut(s) 456
SphI GCATGC 1 cut(s) 276
Sse9I AATT 4 cut(s) 219, 297, 306, 651
SsiI CCGC 3 cut(s) 10, 33, 36
SspMI CTAG 3 cut(s) 69, 302, 676
SstI GAGCTC 1 cut(s) 18
StyD4I CCNGG 3 cut(s) 21, 51, 140
StyI CCWWGG 1 cut(s) 353
TaqI TCGA 3 cut(s) 114, 122, 168
TasI AATT 4 cut(s) 219, 297, 306, 651
TauI GCSGC 1 cut(s) 38
TfiI GAWTC 1 cut(s) 442
Tru1I TTAA 1 cut(s) 560
Tru9I TTAA 1 cut(s) 560
TseFI GTSAC 1 cut(s) 475
TseI GCWGC 1 cut(s) 455
Tsp45I GTSAC 1 cut(s) 475
TspDTI ATGAA 2 cut(s) 17, 202
XapI RAATTY 1 cut(s) 651
XceI RCATGY 1 cut(s) 276
XspI CTAG 3 cut(s) 69, 302, 676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.