MD14G1161500.v1.1

Protein of unknown function (DUF3537)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0014739
Physical Location & Seq
Forward (+)
25622321 .. 25625361
3041 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1161500.v1.1.491

Sequence Viewer

Length: 1296 bp
ATGGCAATCCAAATCCCACTGATAGACACATCAGAGCAAAACCCACCTGAAGATGCCAAACTTGATCACTCCCTCCACAGATTTGAGACATTTCTGAGACTCTTTGGCTTCTGCCAATACTCTTTCTGGAGCTTCATCCTCTCCTGGCTTGCATTTCTCCTTGTGGGTGTTGCAGTTCCTATCATGCTTGCCGAAATCATTGATTGCGCCGATTGCGAAAAGTATGAGATCAAAAGGATTGAGCTTGAGATTCTGGTTTCTGTGTCTCTGGTGGCTGCCATTTCTTTGGGTTGTGTTTCTCACAACATCAGAAAGTATGGAGTGAGGAAGTTCTTGTTTGTGGATAGGTACCATGGCCTTATGCTTCTGTTTCGTGATGAGTACGTTCAAAGGATCAACAAATTCTACCGCTTGCTGGCTGCGTGGGTGTTACCTTGCTTCCTTCTGAAGGCGGCTCGAGAAATCACTATTGTTCTCGTTCTTCATCGCGATCCATGGTGGAAATCTGTTGTCGTACTGCTTGCTGTGCTCGTGTCGTGGACGTATTCGAGCATCATTCTCCTATTAGGCAGTGCTTTGTTCAACTTGGTTTGCAATTTGCATATGATTCACTTTGAGAACTATGGAAAACTTTTAGAAAGGGACTTGGATGTTTCTGCATACATTGACGAACATATTCGTCTAACGCATTACCTATCGAAGATCAGCCACAGATTCCGAATCTATCTTTTTCTTCAGCTCTTAGTTGTGACAGCAAGCCAGTTTGTGGCTCTACTGCAGACAACATCAAACAGCGGGACCATCAACCTCATCAATGGCGGCGATTTTGTAGTCTCTTCCATTGTTGAGGTAGGTGGGCTAGTTATATGCCTGCATGCAGCTTCGAAGATTTCACACAGAGCTCAGGCGCTTGCATCGGTGACTAGCAGATGGCATGCTATGGTCACTTGCAGCTCCAGCGATGCGTCTCAACCAGGAACCAATAATAGCTCCGGAAACTTGGGAATCACCAACAGTTCCGGGAACTTGGAGATTTCTTATGCTGCTTCACTGCCGATAAGCTACTCAAAAACTGATCTGGGGGCAGTTGACTATGTTCCAACGCCTACAAATACACAATTGGCCTCATACATGTCCCTCTATCAGAAAAGACAATCTTTTGTGACCTACATGCAGTCCAACCCTAGTGGAATTACCCTCTATGGATGGACGATTGACCGGGGACTCGTTGCCACCATCTGCTTCATTGAGGTCACACTGGCTATGTTCGTGCTCGGGAAGACGATAACGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3537

Protein Analysis

432

Amino Acids

48.38

Weight (kDa)

7.62

Isoelectric Point (pI)

31.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3537 PF12056 24 - 416 1.7e-138 Protein of unknown function (DUF3537)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014739)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g07730
malus_domestica MD14G1161500.v1.1
prunus_persica Prupe.5G154400_v2.0.a1
pyrus_communis pycom14g13440
rosa_chinensis RchiOBHm_Chr7g0190691
rosa_laevigata RLG00000004503
rosa_multiflora Rmu_sc0006709.1_g000002 Rmu_sc0014053.1_g000002 Rmu_sc0014053.1_g000003 Rmu_sc0026963.1_g000001
rosa_roxburghii Rroxscaffold_3G00264400
rosa_rugosa Rorug06G0506500
rosa_samantha Rh7AG113000 Rh7BG115500 Rh7CG118000 Rh7DG116900
rosa_wichuraiana Rw7G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 348
AccB1I GGYRCC 1 cut(s) 348
AccB7I CCANNNNNTGG 1 cut(s) 766
AccII CGCG 1 cut(s) 489
AccIII TCCGGA 1 cut(s) 992
AciI CCGC 4 cut(s) 409, 452, 795, 819
AclI AACGTT 1 cut(s) 1289
AclWI GGATC 2 cut(s) 401, 485
AcsI RAATTY 1 cut(s) 401
AcuI CTGAAG 3 cut(s) 69, 467, 719
AfaI GTAC 3 cut(s) 350, 383, 516
AfiI CCNNNNNNNGG 3 cut(s) 415, 448, 766
AflIII ACRYGT 1 cut(s) 1131
AgsI TTSAA 2 cut(s) 389, 583
AjnI CCWGG 2 cut(s) 143, 973
AluBI AGCT 8 cut(s) 132, 244, 739, 881, 902, 954, 990, 1062
AluI AGCT 8 cut(s) 132, 244, 739, 881, 902, 954, 990, 1062
Alw21I GWGCWC 3 cut(s) 531, 904, 1275
Alw26I GTCTC 5 cut(s) 80, 91, 270, 838, 972
AlwI GGATC 2 cut(s) 401, 485
Ama87I CYCGRG 2 cut(s) 456, 1274
Aor13HI TCCGGA 1 cut(s) 992
AoxI GGCC 2 cut(s) 355, 1122
ApeKI GCWGC 5 cut(s) 275, 419, 878, 951, 1043
ApoI RAATTY 1 cut(s) 401
Asp700I GAANNNNTTC 1 cut(s) 675
Asp718I GGTACC 1 cut(s) 348
AspLEI GCGC 2 cut(s) 209, 910
AspS9I GGNCC 1 cut(s) 798
AsuC2I CCSGG 2 cut(s) 1021, 1220
AsuHPI GGTGA 2 cut(s) 931, 1000
AsuII TTCGAA 1 cut(s) 884
AvaI CYCGRG 2 cut(s) 456, 1274
AvaII GGWCC 1 cut(s) 798
BanI GGYRCC 1 cut(s) 348
BanII GRGCYC 1 cut(s) 904
BauI CACGAG 1 cut(s) 530
BbsI GAAGAC 1 cut(s) 1286
Bbv12I GWGCWC 3 cut(s) 531, 904, 1275
BbvI GCAGC 5 cut(s) 262, 406, 890, 963, 1030
BccI CCATC 4 cut(s) 809, 924, 1200, 1244
BciT130I CCWGG 2 cut(s) 145, 975
BclI TGATCA 1 cut(s) 64
BcnI CCSGG 2 cut(s) 1021, 1220
BcoDI GTCTC 5 cut(s) 80, 91, 270, 838, 972
BfaI CTAG 3 cut(s) 860, 924, 1185
BfmI CTRYAG 1 cut(s) 776
BfoI RGCGCY 1 cut(s) 911
BisI GCNGC 7 cut(s) 276, 420, 453, 820, 879, 952, 1044
BlsI GCNGC 7 cut(s) 277, 421, 454, 821, 880, 953, 1045
Bme1390I CCNGG 4 cut(s) 145, 975, 1021, 1220
Bme18I GGWCC 1 cut(s) 798
BmeT110I CYCGRG 2 cut(s) 456, 1274
BmgT120I GGNCC 1 cut(s) 798
BmiI GGNNCC 3 cut(s) 350, 799, 979
BmrFI CCNGG 4 cut(s) 145, 975, 1021, 1220
BmsI GCATC 4 cut(s) 43, 561, 923, 952
BpiI GAAGAC 1 cut(s) 1286
BpmI CTGGAG 2 cut(s) 148, 940
Bpu10I CCTNAGC 1 cut(s) 903
Bpu14I TTCGAA 1 cut(s) 884
BpuEI CTTGAG 1 cut(s) 266
BpuMI CCSGG 2 cut(s) 1021, 1220
BsaJI CCNNGG 3 cut(s) 352, 494, 1219
BsaWI WCCGGW 1 cut(s) 992
Bsc4I CCNNNNNNNGG 3 cut(s) 415, 448, 766
Bse1I ACTGG 2 cut(s) 760, 1263
BseAI TCCGGA 1 cut(s) 992
BseBI CCWGG 2 cut(s) 145, 975
BseDI CCNNGG 3 cut(s) 352, 494, 1219
BseGI GGATG 3 cut(s) 135, 655, 1211
BseLI CCNNNNNNNGG 3 cut(s) 415, 448, 766
BseMII CTCAG 2 cut(s) 86, 917
BseNI ACTGG 2 cut(s) 760, 1263
BseXI GCAGC 5 cut(s) 262, 406, 890, 963, 1030
Bsh1236I CGCG 1 cut(s) 489
BshFI GGCC 2 cut(s) 357, 1124
BshNI GGYRCC 1 cut(s) 348
BsiHKAI GWGCWC 3 cut(s) 531, 904, 1275
BsiHKCI CYCGRG 2 cut(s) 456, 1274
BsiSI CCGG 3 cut(s) 993, 1020, 1219
BslFI GGGAC 4 cut(s) 656, 811, 1120, 1236
BslI CCNNNNNNNGG 3 cut(s) 415, 448, 766
BsmAI GTCTC 5 cut(s) 80, 91, 270, 838, 972
BsmBI CGTCTC 1 cut(s) 972
BsmFI GGGAC 4 cut(s) 656, 811, 1120, 1236
BsnI GGCC 2 cut(s) 357, 1124
BsoBI CYCGRG 2 cut(s) 456, 1274
Bsp119I TTCGAA 1 cut(s) 884
Bsp1286I GDGCHC 3 cut(s) 531, 904, 1275
Bsp13I TCCGGA 1 cut(s) 992
Bsp143I GATC 6 cut(s) 64, 228, 393, 490, 702, 1075
Bsp19I CCATGG 2 cut(s) 352, 494
Bsp68I TCGCGA 1 cut(s) 489
BspACI CCGC 4 cut(s) 409, 452, 795, 819
BspANI GGCC 2 cut(s) 357, 1124
BspCNI CTCAG 2 cut(s) 87, 916
BspEI TCCGGA 1 cut(s) 992
BspFNI CGCG 1 cut(s) 489
BspLI GGNNCC 3 cut(s) 350, 799, 979
BspMAI CTGCAG 1 cut(s) 780
BspPI GGATC 2 cut(s) 401, 485
BspT104I TTCGAA 1 cut(s) 884
BspT107I GGYRCC 1 cut(s) 348
BsrI ACTGG 2 cut(s) 760, 1263
BssECI CCNNGG 3 cut(s) 352, 494, 1219
BssMI GATC 6 cut(s) 64, 228, 393, 490, 702, 1075
BssSI CACGAG 1 cut(s) 530
BssT1I CCWWGG 2 cut(s) 352, 494
Bst2BI CACGAG 1 cut(s) 530
Bst2UI CCWGG 2 cut(s) 145, 975
Bst4CI ACNGT 1 cut(s) 1016
Bst6I CTCTTC 1 cut(s) 841
BstBI TTCGAA 1 cut(s) 884
BstDEI CTNAG 3 cut(s) 95, 742, 903
BstDSI CCRYGG 2 cut(s) 352, 494
BstENI CCTNNNNNAGG 1 cut(s) 446
BstF5I GGATG 3 cut(s) 135, 655, 1211
BstFNI CGCG 1 cut(s) 489
BstH2I RGCGCY 1 cut(s) 911
BstHHI GCGC 2 cut(s) 209, 910
BstKTI GATC 6 cut(s) 67, 231, 396, 493, 705, 1078
BstMAI GTCTC 5 cut(s) 80, 91, 270, 838, 972
BstMBI GATC 6 cut(s) 64, 228, 393, 490, 702, 1075
BstMWI GCNNNNNNNGC 3 cut(s) 213, 526, 957
BstNI CCWGG 2 cut(s) 145, 975
BstNSI RCATGY 4 cut(s) 878, 938, 1135, 1174
BstSCI CCNGG 4 cut(s) 143, 973, 1019, 1218
BstSFI CTRYAG 1 cut(s) 776
BstUI CGCG 1 cut(s) 489
BstV1I GCAGC 5 cut(s) 262, 406, 890, 963, 1030
BstV2I GAAGAC 1 cut(s) 1286
BstXI CCANNNNNNTGG 1 cut(s) 286
BsuRI GGCC 2 cut(s) 357, 1124
BtgI CCRYGG 2 cut(s) 352, 494
BtgZI GCGATG 2 cut(s) 470, 975
BtsCI GGATG 3 cut(s) 135, 655, 1211
BtsI GCAGTG 2 cut(s) 577, 1049
BtsIMutI CAGTG 4 cut(s) 17, 577, 1049, 1256
BtuMI TCGCGA 1 cut(s) 489
CfoI GCGC 2 cut(s) 209, 910
Cfr13I GGNCC 1 cut(s) 798
CseI GACGC 1 cut(s) 954
Csp6I GTAC 3 cut(s) 349, 382, 515
CspCI CAANNNNNGTGG 2 cut(s) 1168, 1203
CviAII CATG 7 cut(s) 184, 353, 495, 875, 935, 1132, 1171
CviQI GTAC 3 cut(s) 349, 382, 515
DdeI CTNAG 3 cut(s) 95, 742, 903
DpnI GATC 6 cut(s) 66, 230, 395, 492, 704, 1077
DpnII GATC 6 cut(s) 64, 228, 393, 490, 702, 1075
Eam1104I CTCTTC 1 cut(s) 841
EarI CTCTTC 1 cut(s) 841
Ecl136II GAGCTC 1 cut(s) 902
Eco130I CCWWGG 2 cut(s) 352, 494
Eco24I GRGCYC 1 cut(s) 904
Eco47I GGWCC 1 cut(s) 798
Eco53kI GAGCTC 1 cut(s) 902
Eco57I CTGAAG 3 cut(s) 69, 467, 719
Eco88I CYCGRG 2 cut(s) 456, 1274
EcoICRI GAGCTC 1 cut(s) 902
EcoNI CCTNNNNNAGG 1 cut(s) 446
EcoRII CCWGG 2 cut(s) 143, 973
EcoT14I CCWWGG 2 cut(s) 352, 494
EcoT38I GRGCYC 1 cut(s) 904
ErhI CCWWGG 2 cut(s) 352, 494
Esp3I CGTCTC 1 cut(s) 972
FaeI CATG 7 cut(s) 187, 356, 498, 878, 938, 1135, 1174
FalI AAGNNNNNCTT 2 cut(s) 1141, 1173
FaqI GGGAC 4 cut(s) 656, 811, 1120, 1236
FatI CATG 7 cut(s) 183, 352, 494, 874, 934, 1131, 1170
FauI CCCGC 1 cut(s) 788
FauNDI CATATG 1 cut(s) 603
FbaI TGATCA 1 cut(s) 64
Fnu4HI GCNGC 7 cut(s) 276, 420, 453, 820, 879, 952, 1044
FokI GGATG 3 cut(s) 122, 662, 1218
FriOI GRGCYC 1 cut(s) 904
Fsp4HI GCNGC 7 cut(s) 276, 420, 453, 820, 879, 952, 1044
FspBI CTAG 3 cut(s) 860, 924, 1185
GlaI GCGC 2 cut(s) 208, 909
GluI GCNGC 7 cut(s) 276, 420, 453, 820, 879, 952, 1044
GsuI CTGGAG 2 cut(s) 148, 940
HaeII RGCGCY 1 cut(s) 911
HaeIII GGCC 2 cut(s) 357, 1124
HapII CCGG 3 cut(s) 993, 1020, 1219
HgaI GACGC 1 cut(s) 954
HhaI GCGC 2 cut(s) 209, 910
Hin1II CATG 7 cut(s) 187, 356, 498, 878, 938, 1135, 1174
Hin6I GCGC 2 cut(s) 207, 908
HinP1I GCGC 2 cut(s) 207, 908
HincII GTYRAC 1 cut(s) 1090
HindII GTYRAC 1 cut(s) 1090
HinfI GANTC 7 cut(s) 99, 250, 607, 714, 720, 1005, 1224
HpaII CCGG 3 cut(s) 993, 1020, 1219
HphI GGTGA 2 cut(s) 931, 1000
Hpy166II GTNNAC 2 cut(s) 540, 1090
Hpy188I TCNGA 7 cut(s) 34, 96, 311, 447, 719, 1146, 1295
Hpy188III TCNNGA 6 cut(s) 127, 374, 458, 488, 993, 1276
Hpy8I GTNNAC 2 cut(s) 540, 1090
HpyAV CCTTC 2 cut(s) 442, 452
HpyCH4III ACNGT 1 cut(s) 1016
HpyCH4IV ACGT 3 cut(s) 384, 542, 1289
HpyF10VI GCNNNNNNNGC 3 cut(s) 213, 526, 957
HpyF3I CTNAG 3 cut(s) 95, 742, 903
HpySE526I ACGT 3 cut(s) 384, 542, 1289
Hsp92II CATG 7 cut(s) 187, 356, 498, 878, 938, 1135, 1174
HspAI GCGC 2 cut(s) 207, 908
Kpn2I TCCGGA 1 cut(s) 992
KpnI GGTACC 1 cut(s) 352
Ksp22I TGATCA 1 cut(s) 64
Kzo9I GATC 6 cut(s) 64, 228, 393, 490, 702, 1075
LmnI GCTCC 3 cut(s) 129, 959, 995
Lsp1109I GCAGC 5 cut(s) 262, 406, 890, 963, 1030
LweI GCATC 4 cut(s) 43, 561, 923, 952
MaeI CTAG 3 cut(s) 860, 924, 1185
MaeII ACGT 3 cut(s) 384, 542, 1289
MaeIII GTNAC 6 cut(s) 429, 748, 919, 943, 1162, 1252
MalI GATC 6 cut(s) 66, 230, 395, 492, 704, 1077
MboI GATC 6 cut(s) 64, 228, 393, 490, 702, 1075
MboII GAAGA 7 cut(s) 62, 473, 712, 725, 828, 898, 1291
MfeI CAATTG 1 cut(s) 1118
MhlI GDGCHC 3 cut(s) 531, 904, 1275
MluCI AATT 4 cut(s) 401, 595, 1118, 1191
MlyI GAGTC 2 cut(s) 93, 1218
MmeI TCCRAC 2 cut(s) 1124, 1203
MnlI CCTC 9 cut(s) 83, 149, 318, 818, 841, 1135, 1148, 1208, 1243
MroI TCCGGA 1 cut(s) 992
MroXI GAANNNNTTC 1 cut(s) 675
MspA1I CMGCKG 1 cut(s) 795
MspI CCGG 3 cut(s) 993, 1020, 1219
MspR9I CCNGG 4 cut(s) 145, 975, 1021, 1220
MunI CAATTG 1 cut(s) 1118
MvaI CCWGG 2 cut(s) 145, 975
MvnI CGCG 1 cut(s) 489
MwoI GCNNNNNNNGC 3 cut(s) 213, 526, 957
NciI CCSGG 2 cut(s) 1021, 1220
NcoI CCATGG 2 cut(s) 352, 494
NdeI CATATG 1 cut(s) 603
NdeII GATC 6 cut(s) 64, 228, 393, 490, 702, 1075
NlaIII CATG 7 cut(s) 187, 356, 498, 878, 938, 1135, 1174
NlaIV GGNNCC 3 cut(s) 350, 799, 979
NmuCI GTSAC 5 cut(s) 748, 919, 943, 1162, 1252
NruI TCGCGA 1 cut(s) 489
NspI RCATGY 4 cut(s) 878, 938, 1135, 1174
NspV TTCGAA 1 cut(s) 884
PaeI GCATGC 2 cut(s) 878, 938
PaeR7I CTCGAG 1 cut(s) 456
PciI ACATGT 1 cut(s) 1131
PdmI GAANNNNTTC 1 cut(s) 675
PfeI GAWTC 5 cut(s) 250, 607, 714, 720, 1005
PflMI CCANNNNNTGG 1 cut(s) 766
PfoI TCCNGGA 1 cut(s) 1019
PkrI GCNGC 7 cut(s) 277, 421, 454, 821, 880, 953, 1045
PleI GAGTC 2 cut(s) 93, 1218
PpsI GAGTC 2 cut(s) 93, 1218
PscI ACATGT 1 cut(s) 1131
Psp124BI GAGCTC 1 cut(s) 904
Psp1406I AACGTT 1 cut(s) 1289
Psp6I CCWGG 2 cut(s) 143, 973
PspGI CCWGG 2 cut(s) 143, 973
PspN4I GGNNCC 3 cut(s) 350, 799, 979
PspPI GGNCC 1 cut(s) 798
PstI CTGCAG 1 cut(s) 780
RruI TCGCGA 1 cut(s) 489
RsaI GTAC 3 cut(s) 350, 383, 516
RsaNI GTAC 3 cut(s) 349, 382, 515
SacI GAGCTC 1 cut(s) 904
SatI GCNGC 7 cut(s) 276, 420, 453, 820, 879, 952, 1044
Sau3AI GATC 6 cut(s) 64, 228, 393, 490, 702, 1075
Sau96I GGNCC 1 cut(s) 798
SchI GAGTC 2 cut(s) 93, 1218
ScrFI CCNGG 4 cut(s) 145, 975, 1021, 1220
SduI GDGCHC 3 cut(s) 531, 904, 1275
SfaNI GCATC 4 cut(s) 43, 561, 923, 952
SfcI CTRYAG 1 cut(s) 776
Sfr274I CTCGAG 1 cut(s) 456
SfuI TTCGAA 1 cut(s) 884
SinI GGWCC 1 cut(s) 798
SlaI CTCGAG 1 cut(s) 456
SmlI CTYRAG 2 cut(s) 245, 456
SmoI CTYRAG 2 cut(s) 245, 456
SphI GCATGC 2 cut(s) 878, 938
Sse9I AATT 4 cut(s) 401, 595, 1118, 1191
SsiI CCGC 4 cut(s) 409, 452, 795, 819
SspMI CTAG 3 cut(s) 860, 924, 1185
SstI GAGCTC 1 cut(s) 904
StyD4I CCNGG 4 cut(s) 143, 973, 1019, 1218
StyI CCWWGG 2 cut(s) 352, 494
TaaI ACNGT 1 cut(s) 1016
TaiI ACGT 3 cut(s) 387, 545, 1292
TaqI TCGA 4 cut(s) 457, 548, 698, 884
TasI AATT 4 cut(s) 401, 595, 1118, 1191
TauI GCSGC 2 cut(s) 455, 822
TfiI GAWTC 5 cut(s) 250, 607, 714, 720, 1005
TscAI CASTG 4 cut(s) 24, 577, 1056, 1263
TseFI GTSAC 5 cut(s) 748, 919, 943, 1162, 1252
TseI GCWGC 5 cut(s) 275, 419, 878, 951, 1043
Tsp45I GTSAC 5 cut(s) 748, 919, 943, 1162, 1252
TspDTI ATGAA 3 cut(s) 124, 473, 1234
TspRI CASTG 4 cut(s) 24, 577, 1056, 1263
Van91I CCANNNNNTGG 1 cut(s) 766
VpaK11BI GGWCC 1 cut(s) 798
XagI CCTNNNNNAGG 1 cut(s) 446
XapI RAATTY 1 cut(s) 401
XceI RCATGY 4 cut(s) 878, 938, 1135, 1174
XhoI CTCGAG 1 cut(s) 456
XmnI GAANNNNTTC 1 cut(s) 675
XspI CTAG 3 cut(s) 860, 924, 1185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.