MD14G1162500.v1.1

chitin binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004481
Physical Location & Seq
Forward (+)
25699513 .. 25701965
2453 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1162500.v1.1.491

Sequence Viewer

Length: 1023 bp
ATGGCGACCTCGGCGTTCAAATCAACGACGAAGCGGACGCCGATCGGAGCGTCCAAGGCTCCGCCGGAAGACTCGGCTGCGTTTGATCGGAAGTCGTCTCACCGGCGCTCCCGCAGCCTGAGCTGCTTCTCTCGCGGGTTGCCGGAGCGGACGCCGGCGGCTGACGATTACGATGATAATCCGGTTCCCAGCAGAGGGAGGTTCGTCAACACGGTCAGAGGCTCGGGATTCCCGGAGATCAGCCTCGACGACCTCGCCGTCGAGCTATTCGACTCCTCTGCTGACCGGAGCCGCTCGGTTGCGCGGAGCTCCGAAGCTACTCCTGCTGGCAGTGCCTCGCAGAGGCGTGGGAGGTCGGTGTCGAGGCACGGCCCGAGAGTTGGTGGTGGTGGTGGTGGTGATGTGAGGGGCGGTACTGGTAATAATTCAGCTGGAGGAAGGGTAGTTTCGGAAAGTAAGGGTAATTCAAGCCGAAGGCGATCGGTTTCGGTTGCCCGGTATCAGAAGAGCGATTCTGAGAGTGATCTAGATCATACCCACAATCGCAGCACCATTAAGTCGAGGAACTTCGTTAGTGGAAACAACCAGACACCCTTGTCCCGTAAGTCAACAGATTCAAATTTTAGACCAGCTTTGAGACGATCTCTTAGCCAGAAAGATTTTAAGTCCCATGATGGTTACTCTAGCCAGTCTTCGGTCCTAACTGATGACGAGGGGACGGATGCTTATTCCAACAAAAATGGGGTTGAGAAAACTATCCGAGCTGTATATTCAGAGAAGAAGGCAGAGCACCCTGCTGGTAATGATGTTAAAAGTGGACTTTATGAAGTAATGCGGAAAGAACTTAGACATGCTGTGGAAGAGATCCGGTCAGAACTTGAAATTGCAAATGAGAAAACTAAATCCGCTGTTTCAGCACCTGGTGATTTACGTTCTAACAGTAAAGATGTACTTCAGGCTGTTTCTTCTTCTATCAGGAAGAATTACTCCTCCAAATTGGAACAGGTATTCACGTTACTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

341

Amino Acids

36.81

Weight (kDa)

9.93

Isoelectric Point (pI)

65.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 257, 595
AccBSI CCGCTC 2 cut(s) 148, 294
AccII CGCG 2 cut(s) 135, 304
AclWI GGATC 1 cut(s) 859
AcsI RAATTY 1 cut(s) 619
AcuI CTGAAG 1 cut(s) 938
AcyI GRCGYC 2 cut(s) 38, 152
AdeI CACNNNGTG 1 cut(s) 923
AfaI GTAC 2 cut(s) 415, 951
AfiI CCNNNNNNNGG 5 cut(s) 194, 195, 342, 380, 694
AgsI TTSAA 4 cut(s) 19, 468, 618, 881
AjnI CCWGG 1 cut(s) 919
AluBI AGCT 7 cut(s) 123, 265, 309, 317, 431, 632, 764
AluI AGCT 7 cut(s) 123, 265, 309, 317, 431, 632, 764
Alw21I GWGCWC 2 cut(s) 311, 792
Alw26I GTCTC 2 cut(s) 102, 631
AlwI GGATC 1 cut(s) 859
AlwNI CAGNNNCTG 1 cut(s) 920
Ama87I CYCGRG 2 cut(s) 223, 373
AoxI GGCC 1 cut(s) 370
ApeKI GCWGC 4 cut(s) 77, 114, 123, 546
ApoI RAATTY 1 cut(s) 619
AspLEI GCGC 2 cut(s) 108, 304
AspS9I GGNCC 2 cut(s) 371, 697
AsuC2I CCSGG 2 cut(s) 233, 496
AsuHPI GGTGA 3 cut(s) 92, 410, 935
AvaI CYCGRG 2 cut(s) 223, 373
AvaII GGWCC 1 cut(s) 697
BanII GRGCYC 1 cut(s) 311
BbsI GAAGAC 2 cut(s) 75, 684
Bbv12I GWGCWC 2 cut(s) 311, 792
BbvI GCAGC 4 cut(s) 64, 110, 126, 558
BccI CCATC 1 cut(s) 668
BceAI ACGGC 2 cut(s) 242, 385
BcgI CGANNNNNNTGC 2 cut(s) 260, 294
BciT130I CCWGG 1 cut(s) 921
BcnI CCSGG 2 cut(s) 233, 496
BcoDI GTCTC 2 cut(s) 102, 631
BfaI CTAG 2 cut(s) 527, 684
BfoI RGCGCY 1 cut(s) 109
BisI GCNGC 6 cut(s) 78, 115, 124, 159, 292, 547
BlsI GCNGC 6 cut(s) 79, 116, 125, 160, 293, 548
Bme1390I CCNGG 3 cut(s) 233, 496, 921
Bme18I GGWCC 1 cut(s) 697
BmeT110I CYCGRG 2 cut(s) 223, 373
BmgT120I GGNCC 2 cut(s) 371, 697
BmiI GGNNCC 3 cut(s) 60, 186, 290
BmrFI CCNGG 3 cut(s) 233, 496, 921
BmsI GCATC 1 cut(s) 712
BpiI GAAGAC 2 cut(s) 75, 684
BplI GAGNNNNNCTC 2 cut(s) 628, 660
BpmI CTGGAG 1 cut(s) 453
Bpu10I CCTNAGC 1 cut(s) 119
BpuMI CCSGG 2 cut(s) 233, 496
BsaBI GATNNNNATC 2 cut(s) 177, 528
BsaHI GRCGYC 2 cut(s) 38, 152
BsaJI CCNNGG 2 cut(s) 9, 54
BsaWI WCCGGW 3 cut(s) 181, 285, 867
BsaXI ACNNNNNCTCC 4 cut(s) 92, 122, 426, 456
Bsc4I CCNNNNNNNGG 5 cut(s) 194, 195, 342, 380, 694
Bse118I RCCGGY 2 cut(s) 102, 154
Bse1I ACTGG 2 cut(s) 421, 688
Bse8I GATNNNNATC 2 cut(s) 177, 528
BseBI CCWGG 1 cut(s) 921
BseDI CCNNGG 2 cut(s) 9, 54
BseGI GGATG 1 cut(s) 727
BseJI GATNNNNATC 2 cut(s) 177, 528
BseLI CCNNNNNNNGG 5 cut(s) 194, 195, 342, 380, 694
BseMII CTCAG 2 cut(s) 110, 507
BseNI ACTGG 2 cut(s) 421, 688
BseRI GAGGAG 2 cut(s) 265, 979
BseXI GCAGC 4 cut(s) 64, 110, 126, 558
BseYI CCCAGC 1 cut(s) 188
Bsh1236I CGCG 2 cut(s) 135, 304
Bsh1285I CGRYCG 2 cut(s) 45, 482
BshFI GGCC 1 cut(s) 372
BsiEI CGRYCG 2 cut(s) 45, 482
BsiHKAI GWGCWC 2 cut(s) 311, 792
BsiHKCI CYCGRG 2 cut(s) 223, 373
BsiSI CCGG 9 cut(s) 65, 103, 143, 155, 182, 233, 286, 496, 868
BslFI GGGAC 3 cut(s) 583, 652, 730
BslI CCNNNNNNNGG 5 cut(s) 194, 195, 342, 380, 694
BsmAI GTCTC 2 cut(s) 102, 631
BsmBI CGTCTC 2 cut(s) 102, 631
BsmFI GGGAC 3 cut(s) 583, 652, 730
BsnI GGCC 1 cut(s) 372
BsoBI CYCGRG 2 cut(s) 223, 373
Bsp1286I GDGCHC 2 cut(s) 311, 792
Bsp143I GATC 8 cut(s) 42, 85, 237, 479, 523, 529, 641, 864
BspANI GGCC 1 cut(s) 372
BspCNI CTCAG 2 cut(s) 111, 508
BspFNI CGCG 2 cut(s) 135, 304
BspLI GGNNCC 3 cut(s) 60, 186, 290
BspPI GGATC 1 cut(s) 859
BspQI GCTCTTC 1 cut(s) 500
BsrBI CCGCTC 2 cut(s) 148, 294
BsrFI RCCGGY 2 cut(s) 102, 154
BsrI ACTGG 2 cut(s) 421, 688
BssAI RCCGGY 2 cut(s) 102, 154
BssECI CCNNGG 2 cut(s) 9, 54
BssMI GATC 8 cut(s) 42, 85, 237, 479, 523, 529, 641, 864
BssNI GRCGYC 2 cut(s) 38, 152
BssT1I CCWWGG 1 cut(s) 54
Bst2UI CCWGG 1 cut(s) 921
Bst4CI ACNGT 2 cut(s) 214, 941
Bst6I CTCTTC 2 cut(s) 500, 855
BstACI GRCGYC 2 cut(s) 38, 152
BstC8I GCNNGC 2 cut(s) 156, 328
BstDEI CTNAG 4 cut(s) 119, 516, 647, 845
BstENI CCTNNNNNAGG 1 cut(s) 340
BstF5I GGATG 1 cut(s) 727
BstFNI CGCG 2 cut(s) 135, 304
BstH2I RGCGCY 1 cut(s) 109
BstHHI GCGC 2 cut(s) 108, 304
BstKTI GATC 8 cut(s) 45, 88, 240, 482, 526, 532, 644, 867
BstMAI GTCTC 2 cut(s) 102, 631
BstMBI GATC 8 cut(s) 42, 85, 237, 479, 523, 529, 641, 864
BstMCI CGRYCG 2 cut(s) 45, 482
BstMWI GCNNNNNNNGC 9 cut(s) 11, 56, 114, 120, 123, 132, 323, 332, 914
BstNI CCWGG 1 cut(s) 921
BstNSI RCATGY 1 cut(s) 854
BstSCI CCNGG 3 cut(s) 231, 494, 919
BstUI CGCG 2 cut(s) 135, 304
BstV1I GCAGC 4 cut(s) 64, 110, 126, 558
BstV2I GAAGAC 2 cut(s) 75, 684
BstX2I RGATCY 1 cut(s) 864
BstYI RGATCY 1 cut(s) 864
BsuRI GGCC 1 cut(s) 372
BtsCI GGATG 1 cut(s) 727
BtsI GCAGTG 1 cut(s) 337
BtsIMutI CAGTG 1 cut(s) 337
Cac8I GCNNGC 2 cut(s) 156, 328
CaiI CAGNNNCTG 1 cut(s) 920
CfoI GCGC 2 cut(s) 108, 304
Cfr10I RCCGGY 2 cut(s) 102, 154
Cfr13I GGNCC 2 cut(s) 371, 697
CseI GACGC 3 cut(s) 39, 46, 160
CsiI ACCWGGT 1 cut(s) 919
Csp6I GTAC 2 cut(s) 414, 950
CviAII CATG 2 cut(s) 671, 851
CviQI GTAC 2 cut(s) 414, 950
DdeI CTNAG 4 cut(s) 119, 516, 647, 845
DpnI GATC 8 cut(s) 44, 87, 239, 481, 525, 531, 643, 866
DpnII GATC 8 cut(s) 42, 85, 237, 479, 523, 529, 641, 864
DraIII CACNNNGTG 1 cut(s) 923
DrdI GACNNNNNNGTC 2 cut(s) 257, 595
DseDI GACNNNNNNGTC 2 cut(s) 257, 595
Eam1104I CTCTTC 2 cut(s) 500, 855
EarI CTCTTC 2 cut(s) 500, 855
EciI GGCGGA 1 cut(s) 51
Ecl136II GAGCTC 1 cut(s) 309
Eco130I CCWWGG 1 cut(s) 54
Eco24I GRGCYC 1 cut(s) 311
Eco47I GGWCC 1 cut(s) 697
Eco53kI GAGCTC 1 cut(s) 309
Eco57I CTGAAG 1 cut(s) 938
Eco88I CYCGRG 2 cut(s) 223, 373
EcoICRI GAGCTC 1 cut(s) 309
EcoNI CCTNNNNNAGG 1 cut(s) 340
EcoRII CCWGG 1 cut(s) 919
EcoT14I CCWWGG 1 cut(s) 54
EcoT38I GRGCYC 1 cut(s) 311
ErhI CCWWGG 1 cut(s) 54
Esp3I CGTCTC 2 cut(s) 102, 631
FaeI CATG 2 cut(s) 674, 854
FaiI YATR 6 cut(s) 534, 672, 769, 825, 852, 1021
FalI AAGNNNNNCTT 2 cut(s) 936, 968
FaqI GGGAC 3 cut(s) 583, 652, 730
FatI CATG 2 cut(s) 670, 850
FauI CCCGC 2 cut(s) 119, 128
Fnu4HI GCNGC 6 cut(s) 78, 115, 124, 159, 292, 547
FokI GGATG 1 cut(s) 734
FriOI GRGCYC 1 cut(s) 311
Fsp4HI GCNGC 6 cut(s) 78, 115, 124, 159, 292, 547
FspBI CTAG 2 cut(s) 527, 684
GlaI GCGC 2 cut(s) 107, 303
GluI GCNGC 6 cut(s) 78, 115, 124, 159, 292, 547
GsaI CCCAGC 1 cut(s) 192
GsuI CTGGAG 1 cut(s) 453
HaeII RGCGCY 1 cut(s) 109
HaeIII GGCC 1 cut(s) 372
HapII CCGG 9 cut(s) 65, 103, 143, 155, 182, 233, 286, 496, 868
HgaI GACGC 3 cut(s) 39, 46, 160
HhaI GCGC 2 cut(s) 108, 304
Hin1I GRCGYC 2 cut(s) 38, 152
Hin1II CATG 2 cut(s) 674, 854
Hin6I GCGC 2 cut(s) 106, 302
HinP1I GCGC 2 cut(s) 106, 302
HincII GTYRAC 2 cut(s) 208, 609
HindII GTYRAC 2 cut(s) 208, 609
HinfI GANTC 5 cut(s) 71, 228, 272, 512, 614
HpaII CCGG 9 cut(s) 65, 103, 143, 155, 182, 233, 286, 496, 868
HphI GGTGA 3 cut(s) 92, 410, 935
Hpy166II GTNNAC 3 cut(s) 208, 609, 818
Hpy188III TCNNGA 3 cut(s) 225, 527, 976
Hpy8I GTNNAC 3 cut(s) 208, 609, 818
Hpy99I CGWCG 3 cut(s) 31, 251, 263
HpyAV CCTTC 3 cut(s) 432, 468, 775
HpyCH4III ACNGT 2 cut(s) 214, 941
HpyCH4IV ACGT 2 cut(s) 931, 1013
HpyCH4V TGCA 1 cut(s) 887
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 56, 114, 120, 123, 132, 323, 332, 914
HpyF3I CTNAG 4 cut(s) 119, 516, 647, 845
HpySE526I ACGT 2 cut(s) 931, 1013
Hsp92I GRCGYC 2 cut(s) 38, 152
Hsp92II CATG 2 cut(s) 674, 854
HspAI GCGC 2 cut(s) 106, 302
KroI GCCGGC 1 cut(s) 154
KroNI GCCGGC 1 cut(s) 156
Kzo9I GATC 8 cut(s) 42, 85, 237, 479, 523, 529, 641, 864
LguI GCTCTTC 1 cut(s) 500
LmnI GCTCC 7 cut(s) 47, 64, 113, 145, 288, 306, 314
Lsp1109I GCAGC 4 cut(s) 64, 110, 126, 558
LweI GCATC 1 cut(s) 712
MabI ACCWGGT 1 cut(s) 919
MaeI CTAG 2 cut(s) 527, 684
MaeII ACGT 2 cut(s) 931, 1013
MaeIII GTNAC 2 cut(s) 677, 1014
MalI GATC 8 cut(s) 44, 87, 239, 481, 525, 531, 643, 866
MbiI CCGCTC 2 cut(s) 148, 294
MboI GATC 8 cut(s) 42, 85, 237, 479, 523, 529, 641, 864
MboII GAAGA 8 cut(s) 80, 517, 684, 790, 872, 957, 960, 991
MflI RGATCY 1 cut(s) 864
MhlI GDGCHC 2 cut(s) 311, 792
MluCI AATT 6 cut(s) 424, 463, 619, 882, 982, 995
MlyI GAGTC 2 cut(s) 65, 266
MmeI TCCRAC 1 cut(s) 756
MreI CGCCGGCG 1 cut(s) 154
MroNI GCCGGC 1 cut(s) 154
MseI TTAA 3 cut(s) 555, 663, 810
MspA1I CMGCKG 2 cut(s) 431, 908
MspI CCGG 9 cut(s) 65, 103, 143, 155, 182, 233, 286, 496, 868
MspR9I CCNGG 3 cut(s) 233, 496, 921
MvaI CCWGG 1 cut(s) 921
MvnI CGCG 2 cut(s) 135, 304
MwoI GCNNNNNNNGC 9 cut(s) 11, 56, 114, 120, 123, 132, 323, 332, 914
NaeI GCCGGC 1 cut(s) 156
NciI CCSGG 2 cut(s) 233, 496
NdeII GATC 8 cut(s) 42, 85, 237, 479, 523, 529, 641, 864
NgoMIV GCCGGC 1 cut(s) 154
NlaIII CATG 2 cut(s) 674, 854
NlaIV GGNNCC 3 cut(s) 60, 186, 290
NmeAIII GCCGAG 1 cut(s) 53
NspI RCATGY 1 cut(s) 854
PciSI GCTCTTC 1 cut(s) 500
PcsI WCGNNNNNNNCGW 2 cut(s) 255, 267
PdiI GCCGGC 1 cut(s) 156
PfeI GAWTC 3 cut(s) 228, 512, 614
PfoI TCCNGGA 1 cut(s) 231
PkrI GCNGC 6 cut(s) 79, 116, 125, 160, 293, 548
Ple19I CGATCG 2 cut(s) 45, 482
PleI GAGTC 2 cut(s) 65, 266
PpsI GAGTC 2 cut(s) 65, 266
Psp124BI GAGCTC 1 cut(s) 311
Psp6I CCWGG 1 cut(s) 919
PspFI CCCAGC 1 cut(s) 188
PspGI CCWGG 1 cut(s) 919
PspN4I GGNNCC 3 cut(s) 60, 186, 290
PspPI GGNCC 2 cut(s) 371, 697
PstNI CAGNNNCTG 1 cut(s) 920
PsuI RGATCY 1 cut(s) 864
PvuI CGATCG 2 cut(s) 45, 482
PvuII CAGCTG 1 cut(s) 431
RsaI GTAC 2 cut(s) 415, 951
RsaNI GTAC 2 cut(s) 414, 950
SacI GAGCTC 1 cut(s) 311
SapI GCTCTTC 1 cut(s) 500
SaqAI TTAA 3 cut(s) 555, 663, 810
SatI GCNGC 6 cut(s) 78, 115, 124, 159, 292, 547
Sau3AI GATC 8 cut(s) 42, 85, 237, 479, 523, 529, 641, 864
Sau96I GGNCC 2 cut(s) 371, 697
SchI GAGTC 2 cut(s) 65, 266
ScrFI CCNGG 3 cut(s) 233, 496, 921
SduI GDGCHC 2 cut(s) 311, 792
SexAI ACCWGGT 1 cut(s) 919
SfaNI GCATC 1 cut(s) 712
SgrAI CRCCGGYG 2 cut(s) 102, 154
SinI GGWCC 1 cut(s) 697
Sse9I AATT 6 cut(s) 424, 463, 619, 882, 982, 995
SspMI CTAG 2 cut(s) 527, 684
SstI GAGCTC 1 cut(s) 311
StyD4I CCNGG 3 cut(s) 231, 494, 919
StyI CCWWGG 1 cut(s) 54
TaaI ACNGT 2 cut(s) 214, 941
TaiI ACGT 2 cut(s) 934, 1016
TaqI TCGA 5 cut(s) 246, 261, 270, 362, 560
TaqII GACCGA 1 cut(s) 685
TasI AATT 6 cut(s) 424, 463, 619, 882, 982, 995
TatI WGTACW 1 cut(s) 949
TauI GCSGC 2 cut(s) 161, 294
TfiI GAWTC 3 cut(s) 228, 512, 614
Tru1I TTAA 3 cut(s) 555, 663, 810
Tru9I TTAA 3 cut(s) 555, 663, 810
TscAI CASTG 1 cut(s) 337
TseI GCWGC 4 cut(s) 77, 114, 123, 546
TspDTI ATGAA 1 cut(s) 840
TspGWI ACGGA 1 cut(s) 734
TspRI CASTG 1 cut(s) 337
VpaK11BI GGWCC 1 cut(s) 697
XagI CCTNNNNNAGG 1 cut(s) 340
XapI RAATTY 1 cut(s) 619
XbaI TCTAGA 1 cut(s) 526
XceI RCATGY 1 cut(s) 854
XspI CTAG 2 cut(s) 527, 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.