MD14G1167900.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0016330
Physical Location & Seq
Reverse (-)
26106279 .. 26108051
1773 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1167900.v1.1.491

Sequence Viewer

Length: 1704 bp
ATGAACACAGCAACGGTCGCCAAAAACCATGCCAACAACAAAGCAGACGGAGCCGCCTCTCTGCTAAAATCCTGCACATCGCTCCGCCACCTCAAACAAATCCACGCCCACATCGTCCGTTCAAACCTCCACGGGAACGCCTCCTTAGTCTCCACTCTCATCTCTCTCTACGCCTCTCTCTCCTCCTCGCCTCGTTATACCCACCTCGTCTTCACCTCTTTCCCCAACCCAAATCTATCCCTCTTCAACCACGCTGTCAGAGCTTTCTCTAAGATCTCCGTGCTCTGTTCCGAGGCGGTGAGTTTGTATTCGCAGATGGTCTCGGTTGGGATTAGACCCGATAACTATACGTACCCTTTCGTGCTTAATTCGTGCGCTGCGATGAGTGATCTCTGTGGTGGGATTGAAGTCCATGGACGCATTGTGAAAATGGGTTTTGGGTTTTGTGTTCCGGTTTCGAACGCTTTGATTGATATGTATGGGAAATGTGGGGAGGTGTTGAGGGCGCGGAAGGTGTTTGATGAAATGTATGAGAGAGATGTTGTTTCTTATAATGCGGTTTTGGGGGCTCATGCGAGAGGTGGGGTGGATATGGAGAGTGCTTTGGTTTTGTTTGAGGGGATGTTAGGAAAAAATGTGATATCTTGGAACGCCATGGTTGTAGGTTATGTAAATAGTGGGGATCTGGTTTCGGCAAGGGCTGTGTTTGATAGGATGCCTGAGAGGAATGCTGTTTCTTGGACTACAATGTTGGTGGGGTATACTAAAAGCGGTCTTATGGATAAGGCTAAGTGTCTCTTTGAAGAAATGCCTGAGCAAGGTTTGATTTGTTGGACGGCTATGATATCAGGGTATTCACAAAATGGGAGGTCAAGTGAAGCGCTTTCACTTTTCCGGAGAATGGAAAGGGCACGAGTAAAGCCAGATGCTTTCACCATGACAGCGGTGATCTCTGCATTGGCACAGTTGGGTCGTGCAGATCTGGCGAACTGGATTGGAACATATGTGGATAGAGAAGGCATAGAACAGAATGAACAAGTGCTTACTGCACTAGTTGACATGCATGCCAAATGTGGGAATATGGAAGAGGCATGCGCCATATTTGAGAAGATCCCCCAAAAAGATGTTTTTTCTTACAGTGCAATAATCACAGGTCTTGCTTCAAATGGTCATGGGGTTAAAGCGCTGGAGATCTTCCAGAGAATGCTGGCAGAAGATATTGAACCTGATCGGGTTACATTTGTTGGGGTATTCACGGCCTGTTGCCATGCAGGACTTGTTGAAGATGGAATGAGGTACTGGGAAAGCATGGTTAAAGATTACAACATTGAGCCTGATGCTGATCACTATACTTGCATAGTTGATATGCTGGGACGAGCTGGGAAACTCAATGAGGCCTGCGATTTGGTCAAGGGAATGCCTATGGGCCCACAGCCAGGAGCATTAGGAGCCCTTCTTGCTGCATGTAGAACATATGGAAATGTAGAGATTGCAGAAAGTGTTGCTGAGAAACTCTTCAGTTTAGAACCTGGGAATACTGGAAATTATATGCTACTTTCAAGCATTTATGCGTCGAAAGAACAATGGGATGAAGCTAGGAAAGTGCGGGAAGCAATGAACGGGAGAAATGCACTTAAGCTTCCTGGGTGCAGTTGGGTTGAAATTAGCAAAGGTCATGCAGCTCAAACGACAAACCAAACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

568

Amino Acids

61.95

Weight (kDa)

6.29

Isoelectric Point (pI)

34.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 243 - 275 2.8e-06 PPR repeat family
PPR PF01535 245 - 275 1.3e-07 PPR repeat
PPR_2 PF13041 276 - 321 9.3e-12 PPR repeat family
PPR PF01535 277 - 305 6.8e-09 PPR repeat
PPR_2 PF13041 374 - 423 4.2e-10 PPR repeat family
E_motif PF20431 492 - 554 2.6e-18 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016330)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g06640 FvH4_5g06640 FvH4_5g06640
malus_domestica MD06G1162200.v1.1 MD14G1167900.v1.1
prunus_persica Prupe.5G162800_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0192051
rosa_laevigata RLG00000004405
rosa_multiflora Rmu_ssc0000421.1_g000037
rosa_roxburghii Rroxscaffold_3G00263330
rosa_rugosa Rorug06G0512800.1
rosa_samantha Rh7BG124700 Rh7CG127400 Rh7DG126200
rosa_wichuraiana Rw7G010480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 552
AccI GTMKAC 1 cut(s) 761
AccII CGCG 1 cut(s) 508
AccIII TCCGGA 1 cut(s) 894
AciI CCGC 8 cut(s) 54, 85, 296, 508, 557, 771, 944, 1606
AclWI GGATC 2 cut(s) 690, 1105
AcuI CTGAAG 1 cut(s) 1501
AfaI GTAC 2 cut(s) 353, 1298
AfeI AGCGCT 2 cut(s) 882, 1185
AfiI CCNNNNNNNGG 4 cut(s) 818, 901, 1074, 1436
AflII CTTAAG 1 cut(s) 1634
AgsI TTSAA 9 cut(s) 123, 247, 407, 803, 1164, 1223, 1283, 1560, 1661
AhlI ACTAGT 1 cut(s) 1051
AjnI CCWGG 3 cut(s) 1435, 1528, 1642
AluBI AGCT 5 cut(s) 263, 1379, 1595, 1639, 1682
AluI AGCT 5 cut(s) 263, 1379, 1595, 1639, 1682
Alw21I GWGCWC 1 cut(s) 285
Alw26I GTCTC 3 cut(s) 154, 325, 800
AlwI GGATC 2 cut(s) 690, 1105
Aor13HI TCCGGA 1 cut(s) 894
Aor51HI AGCGCT 2 cut(s) 882, 1185
AoxI GGCC 3 cut(s) 1257, 1395, 1426
ApaI GGGCCC 1 cut(s) 1430
ApeKI GCWGC 3 cut(s) 377, 1460, 1679
Asp700I GAANNNNTTC 1 cut(s) 1514
AspLEI GCGC 5 cut(s) 377, 508, 883, 1097, 1186
AspS9I GGNCC 2 cut(s) 1426, 1427
AsuHPI GGTGA 4 cut(s) 205, 310, 925, 958
AsuII TTCGAA 1 cut(s) 458
BaeGI GKGCMC 2 cut(s) 913, 1430
BanII GRGCYC 3 cut(s) 571, 1430, 1453
BauI CACGAG 1 cut(s) 912
BbsI GAAGAC 1 cut(s) 202
Bbv12I GWGCWC 1 cut(s) 285
BbvI GCAGC 3 cut(s) 364, 1447, 1691
BccI CCATC 2 cut(s) 310, 1280
BceAI ACGGC 2 cut(s) 852, 1272
BciT130I CCWGG 3 cut(s) 1437, 1530, 1644
BclI TGATCA 1 cut(s) 1342
BcoDI GTCTC 3 cut(s) 154, 325, 800
BcuI ACTAGT 1 cut(s) 1051
BfaI CTAG 2 cut(s) 1052, 1596
BfoI RGCGCY 2 cut(s) 884, 1187
BfrI CTTAAG 1 cut(s) 1634
BglII AGATCT 3 cut(s) 273, 979, 1191
BisI GCNGC 4 cut(s) 54, 378, 1461, 1680
BlsI GCNGC 4 cut(s) 55, 379, 1462, 1681
Bme1390I CCNGG 3 cut(s) 1437, 1530, 1644
BmgT120I GGNCC 2 cut(s) 1426, 1427
BmiI GGNNCC 3 cut(s) 52, 1428, 1450
BmrFI CCNGG 3 cut(s) 1437, 1530, 1644
BmrI ACTGGG 1 cut(s) 1309
BmsI GCATC 3 cut(s) 705, 916, 1327
BmuI ACTGGG 1 cut(s) 1309
BpiI GAAGAC 1 cut(s) 202
BpmI CTGGAG 1 cut(s) 1208
Bpu10I CCTNAGC 1 cut(s) 813
Bpu14I TTCGAA 1 cut(s) 458
BsaAI YACGTR 1 cut(s) 351
BsaBI GATNNNNATC 1 cut(s) 1341
BsaI GGTCTC 1 cut(s) 325
BsaJI CCNNGG 6 cut(s) 130, 291, 412, 654, 1529, 1643
BsaWI WCCGGW 2 cut(s) 451, 894
BsaXI ACNNNNNCTCC 2 cut(s) 859, 889
Bsc4I CCNNNNNNNGG 4 cut(s) 818, 901, 1074, 1436
Bse1I ACTGG 3 cut(s) 995, 1304, 1543
Bse3DI GCAATG 1 cut(s) 1620
Bse8I GATNNNNATC 1 cut(s) 1341
BseAI TCCGGA 1 cut(s) 894
BseBI CCWGG 3 cut(s) 1437, 1530, 1644
BseDI CCNNGG 6 cut(s) 130, 291, 412, 654, 1529, 1643
BseGI GGATG 3 cut(s) 627, 720, 1594
BseJI GATNNNNATC 1 cut(s) 1341
BseLI CCNNNNNNNGG 4 cut(s) 818, 901, 1074, 1436
BseMI GCAATG 1 cut(s) 1620
BseMII CTCAG 3 cut(s) 711, 804, 1497
BseNI ACTGG 3 cut(s) 995, 1304, 1543
BseRI GAGGAG 2 cut(s) 172, 175
BseSI GKGCMC 2 cut(s) 913, 1430
BseXI GCAGC 3 cut(s) 364, 1447, 1691
BseYI CCCAGC 2 cut(s) 1369, 1379
BsgI GTGCAG 4 cut(s) 58, 996, 1032, 1669
Bsh1236I CGCG 1 cut(s) 508
Bsh1285I CGRYCG 1 cut(s) 18
BshFI GGCC 3 cut(s) 1259, 1397, 1428
BsiEI CGRYCG 1 cut(s) 18
BsiHKAI GWGCWC 1 cut(s) 285
BsiSI CCGG 2 cut(s) 452, 895
BslFI GGGAC 1 cut(s) 1386
BslI CCNNNNNNNGG 4 cut(s) 818, 901, 1074, 1436
BsmAI GTCTC 3 cut(s) 154, 325, 800
BsmFI GGGAC 1 cut(s) 1386
BsmI GAATGC 3 cut(s) 733, 1209, 1422
BsnI GGCC 3 cut(s) 1259, 1397, 1428
Bso31I GGTCTC 1 cut(s) 325
Bsp119I TTCGAA 1 cut(s) 458
Bsp120I GGGCCC 1 cut(s) 1426
Bsp1286I GDGCHC 5 cut(s) 285, 571, 913, 1430, 1453
Bsp13I TCCGGA 1 cut(s) 894
Bsp143I GATC 9 cut(s) 273, 388, 682, 948, 979, 1110, 1191, 1228, 1342
Bsp19I CCATGG 2 cut(s) 412, 654
BspACI CCGC 8 cut(s) 54, 85, 296, 508, 557, 771, 944, 1606
BspANI GGCC 3 cut(s) 1259, 1397, 1428
BspCNI CTCAG 3 cut(s) 712, 805, 1498
BspEI TCCGGA 1 cut(s) 894
BspFNI CGCG 1 cut(s) 508
BspLI GGNNCC 3 cut(s) 52, 1428, 1450
BspPI GGATC 2 cut(s) 690, 1105
BspT104I TTCGAA 1 cut(s) 458
BspTI CTTAAG 1 cut(s) 1634
BspTNI GGTCTC 1 cut(s) 325
BsrDI GCAATG 1 cut(s) 1620
BsrI ACTGG 3 cut(s) 995, 1304, 1543
BssECI CCNNGG 6 cut(s) 130, 291, 412, 654, 1529, 1643
BssMI GATC 9 cut(s) 273, 388, 682, 948, 979, 1110, 1191, 1228, 1342
BssNAI GTATAC 1 cut(s) 762
BssSI CACGAG 1 cut(s) 912
BssT1I CCWWGG 2 cut(s) 412, 654
Bst1107I GTATAC 1 cut(s) 762
Bst2BI CACGAG 1 cut(s) 912
Bst2UI CCWGG 3 cut(s) 1437, 1530, 1644
Bst4CI ACNGT 3 cut(s) 16, 966, 1139
Bst6I CTCTTC 3 cut(s) 248, 1080, 1520
BstAFI CTTAAG 1 cut(s) 1634
BstBAI YACGTR 1 cut(s) 351
BstBI TTCGAA 1 cut(s) 458
BstC8I GCNNGC 4 cut(s) 1065, 1093, 1209, 1399
BstDEI CTNAG 7 cut(s) 145, 270, 720, 789, 813, 1506, 1701
BstDSI CCRYGG 3 cut(s) 130, 412, 654
BstENI CCTNNNNNAGG 1 cut(s) 816
BstF5I GGATG 3 cut(s) 627, 720, 1594
BstFNI CGCG 1 cut(s) 508
BstH2I RGCGCY 2 cut(s) 884, 1187
BstHHI GCGC 5 cut(s) 377, 508, 883, 1097, 1186
BstKTI GATC 9 cut(s) 276, 391, 685, 951, 982, 1113, 1194, 1231, 1345
BstMAI GTCTC 3 cut(s) 154, 325, 800
BstMBI GATC 9 cut(s) 273, 388, 682, 948, 979, 1110, 1191, 1228, 1342
BstMCI CGRYCG 1 cut(s) 18
BstMWI GCNNNNNNNGC 6 cut(s) 17, 50, 260, 983, 1448, 1457
BstNI CCWGG 3 cut(s) 1437, 1530, 1644
BstNSI RCATGY 4 cut(s) 1063, 1067, 1095, 1467
BstSCI CCNGG 3 cut(s) 1435, 1528, 1642
BstSLI GKGCMC 2 cut(s) 913, 1430
BstSNI TACGTA 1 cut(s) 351
BstUI CGCG 1 cut(s) 508
BstV1I GCAGC 3 cut(s) 364, 1447, 1691
BstV2I GAAGAC 1 cut(s) 202
BstX2I RGATCY 5 cut(s) 273, 682, 979, 1110, 1191
BstYI RGATCY 5 cut(s) 273, 682, 979, 1110, 1191
BstZ17I GTATAC 1 cut(s) 762
BsuRI GGCC 3 cut(s) 1259, 1397, 1428
BtgI CCRYGG 3 cut(s) 130, 412, 654
BtgZI GCGATG 2 cut(s) 63, 395
BtsCI GGATG 3 cut(s) 627, 720, 1594
BtsIMutI CAGTG 1 cut(s) 1144
Cac8I GCNNGC 4 cut(s) 1065, 1093, 1209, 1399
CfoI GCGC 5 cut(s) 377, 508, 883, 1097, 1186
Cfr13I GGNCC 2 cut(s) 1426, 1427
CseI GACGC 2 cut(s) 426, 1560
Csp6I GTAC 2 cut(s) 352, 1297
CspCI CAANNNNNGTGG 2 cut(s) 735, 770
CviQI GTAC 2 cut(s) 352, 1297
DdeI CTNAG 7 cut(s) 145, 270, 720, 789, 813, 1506, 1701
DpnI GATC 9 cut(s) 275, 390, 684, 950, 981, 1112, 1193, 1230, 1344
DpnII GATC 9 cut(s) 273, 388, 682, 948, 979, 1110, 1191, 1228, 1342
Eam1104I CTCTTC 3 cut(s) 248, 1080, 1520
EarI CTCTTC 3 cut(s) 248, 1080, 1520
EciI GGCGGA 1 cut(s) 74
Eco105I TACGTA 1 cut(s) 351
Eco130I CCWWGG 2 cut(s) 412, 654
Eco147I AGGCCT 1 cut(s) 1397
Eco24I GRGCYC 3 cut(s) 571, 1430, 1453
Eco31I GGTCTC 1 cut(s) 325
Eco32I GATATC 2 cut(s) 642, 846
Eco47III AGCGCT 2 cut(s) 882, 1185
Eco57I CTGAAG 1 cut(s) 1501
EcoNI CCTNNNNNAGG 1 cut(s) 816
EcoRII CCWGG 3 cut(s) 1435, 1528, 1642
EcoRV GATATC 2 cut(s) 642, 846
EcoT14I CCWWGG 2 cut(s) 412, 654
EcoT22I ATGCAT 1 cut(s) 1065
EcoT38I GRGCYC 3 cut(s) 571, 1430, 1453
ErhI CCWWGG 2 cut(s) 412, 654
FalI AAGNNNNNCTT 2 cut(s) 782, 814
FaqI GGGAC 1 cut(s) 1386
FauI CCCGC 1 cut(s) 1599
FauNDI CATATG 2 cut(s) 1003, 1474
FbaI TGATCA 1 cut(s) 1342
FblI GTMKAC 1 cut(s) 761
Fnu4HI GCNGC 4 cut(s) 54, 378, 1461, 1680
FokI GGATG 3 cut(s) 634, 727, 1601
FriOI GRGCYC 3 cut(s) 571, 1430, 1453
Fsp4HI GCNGC 4 cut(s) 54, 378, 1461, 1680
FspBI CTAG 2 cut(s) 1052, 1596
GlaI GCGC 5 cut(s) 376, 507, 882, 1096, 1185
GluI GCNGC 4 cut(s) 54, 378, 1461, 1680
GsaI CCCAGC 2 cut(s) 1373, 1383
GsuI CTGGAG 1 cut(s) 1208
HaeII RGCGCY 2 cut(s) 884, 1187
HaeIII GGCC 3 cut(s) 1259, 1397, 1428
HapII CCGG 2 cut(s) 452, 895
HgaI GACGC 2 cut(s) 426, 1560
HhaI GCGC 5 cut(s) 377, 508, 883, 1097, 1186
Hin6I GCGC 5 cut(s) 375, 506, 881, 1095, 1184
HinP1I GCGC 5 cut(s) 375, 506, 881, 1095, 1184
HincII GTYRAC 1 cut(s) 1057
HindII GTYRAC 1 cut(s) 1057
HindIII AAGCTT 1 cut(s) 1637
HpaII CCGG 2 cut(s) 452, 895
HphI GGTGA 4 cut(s) 205, 310, 925, 958
Hpy166II GTNNAC 2 cut(s) 762, 1057
Hpy188I TCNGA 2 cut(s) 260, 292
Hpy188III TCNNGA 2 cut(s) 895, 1198
Hpy8I GTNNAC 2 cut(s) 762, 1057
Hpy99I CGWCG 1 cut(s) 1576
HpyAV CCTTC 3 cut(s) 505, 1010, 1463
HpyCH4III ACNGT 3 cut(s) 16, 966, 1139
HpyCH4IV ACGT 1 cut(s) 350
HpyF10VI GCNNNNNNNGC 6 cut(s) 17, 50, 260, 983, 1448, 1457
HpyF3I CTNAG 7 cut(s) 145, 270, 720, 789, 813, 1506, 1701
HpySE526I ACGT 1 cut(s) 350
HspAI GCGC 5 cut(s) 375, 506, 881, 1095, 1184
Kpn2I TCCGGA 1 cut(s) 894
Ksp22I TGATCA 1 cut(s) 1342
Kzo9I GATC 9 cut(s) 273, 388, 682, 948, 979, 1110, 1191, 1228, 1342
LmnI GCTCC 4 cut(s) 50, 87, 1439, 1448
Lsp1109I GCAGC 3 cut(s) 364, 1447, 1691
LweI GCATC 3 cut(s) 705, 916, 1327
MaeI CTAG 2 cut(s) 1052, 1596
MaeII ACGT 1 cut(s) 350
MaeIII GTNAC 1 cut(s) 1234
MalI GATC 9 cut(s) 275, 390, 684, 950, 981, 1112, 1193, 1230, 1344
MboI GATC 9 cut(s) 273, 388, 682, 948, 979, 1110, 1191, 1228, 1342
MboII GAAGA 9 cut(s) 202, 235, 815, 1097, 1120, 1186, 1226, 1295, 1507
MflI RGATCY 5 cut(s) 273, 682, 979, 1110, 1191
MhlI GDGCHC 5 cut(s) 285, 571, 913, 1430, 1453
MluCI AATT 3 cut(s) 367, 1543, 1662
MmeI TCCRAC 1 cut(s) 812
Mph1103I ATGCAT 1 cut(s) 1065
MroI TCCGGA 1 cut(s) 894
MroXI GAANNNNTTC 1 cut(s) 1514
MseI TTAA 4 cut(s) 366, 1179, 1314, 1635
MspA1I CMGCKG 1 cut(s) 944
MspCI CTTAAG 1 cut(s) 1634
MspI CCGG 2 cut(s) 452, 895
MspR9I CCNGG 3 cut(s) 1437, 1530, 1644
Mva1269I GAATGC 3 cut(s) 733, 1209, 1422
MvaI CCWGG 3 cut(s) 1437, 1530, 1644
MvnI CGCG 1 cut(s) 508
MwoI GCNNNNNNNGC 6 cut(s) 17, 50, 260, 983, 1448, 1457
NcoI CCATGG 2 cut(s) 412, 654
NdeI CATATG 2 cut(s) 1003, 1474
NdeII GATC 9 cut(s) 273, 388, 682, 948, 979, 1110, 1191, 1228, 1342
NlaIV GGNNCC 3 cut(s) 52, 1428, 1450
NsiI ATGCAT 1 cut(s) 1065
NspI RCATGY 4 cut(s) 1063, 1067, 1095, 1467
NspV TTCGAA 1 cut(s) 458
PaeI GCATGC 2 cut(s) 1067, 1095
PceI AGGCCT 1 cut(s) 1397
PcsI WCGNNNNNNNCGW 2 cut(s) 111, 377
PctI GAATGC 3 cut(s) 733, 1209, 1422
PdmI GAANNNNTTC 1 cut(s) 1514
PkrI GCNGC 4 cut(s) 55, 379, 1462, 1681
Ppu21I YACGTR 1 cut(s) 351
PsiI TTATAA 1 cut(s) 552
Psp6I CCWGG 3 cut(s) 1435, 1528, 1642
PspFI CCCAGC 2 cut(s) 1369, 1379
PspGI CCWGG 3 cut(s) 1435, 1528, 1642
PspN4I GGNNCC 3 cut(s) 52, 1428, 1450
PspOMI GGGCCC 1 cut(s) 1426
PspPI GGNCC 2 cut(s) 1426, 1427
PsuI RGATCY 5 cut(s) 273, 682, 979, 1110, 1191
RsaI GTAC 2 cut(s) 353, 1298
RsaNI GTAC 2 cut(s) 352, 1297
SaqAI TTAA 4 cut(s) 366, 1179, 1314, 1635
SatI GCNGC 4 cut(s) 54, 378, 1461, 1680
Sau3AI GATC 9 cut(s) 273, 388, 682, 948, 979, 1110, 1191, 1228, 1342
Sau96I GGNCC 2 cut(s) 1426, 1427
ScrFI CCNGG 3 cut(s) 1437, 1530, 1644
SduI GDGCHC 5 cut(s) 285, 571, 913, 1430, 1453
SfaNI GCATC 3 cut(s) 705, 916, 1327
SfuI TTCGAA 1 cut(s) 458
SmlI CTYRAG 1 cut(s) 1634
SmoI CTYRAG 1 cut(s) 1634
SnaBI TACGTA 1 cut(s) 351
SpeI ACTAGT 1 cut(s) 1051
SphI GCATGC 2 cut(s) 1067, 1095
Sse9I AATT 3 cut(s) 367, 1543, 1662
SseBI AGGCCT 1 cut(s) 1397
SsiI CCGC 8 cut(s) 54, 85, 296, 508, 557, 771, 944, 1606
SspMI CTAG 2 cut(s) 1052, 1596
StuI AGGCCT 1 cut(s) 1397
StyD4I CCNGG 3 cut(s) 1435, 1528, 1642
StyI CCWWGG 2 cut(s) 412, 654
TaaI ACNGT 3 cut(s) 16, 966, 1139
TaiI ACGT 1 cut(s) 353
TaqI TCGA 2 cut(s) 458, 1574
TasI AATT 3 cut(s) 367, 1543, 1662
TauI GCSGC 1 cut(s) 56
Tru1I TTAA 4 cut(s) 366, 1179, 1314, 1635
Tru9I TTAA 4 cut(s) 366, 1179, 1314, 1635
TscAI CASTG 1 cut(s) 1144
TseI GCWGC 3 cut(s) 377, 1460, 1679
TspDTI ATGAA 5 cut(s) 17, 537, 1047, 1605, 1631
TspGWI ACGGA 3 cut(s) 63, 107, 268
TspRI CASTG 1 cut(s) 1144
Vha464I CTTAAG 1 cut(s) 1634
XagI CCTNNNNNAGG 1 cut(s) 816
XceI RCATGY 4 cut(s) 1063, 1067, 1095, 1467
XmiI GTMKAC 1 cut(s) 761
XmnI GAANNNNTTC 1 cut(s) 1514
XspI CTAG 2 cut(s) 1052, 1596
Zsp2I ATGCAT 1 cut(s) 1065
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.