Rroxscaffold_3G00263330

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0016330
Physical Location & Seq
Forward (+)
57291136 .. 57292833
1698 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00263330.1

Sequence Viewer

Length: 1698 bp
ATGATCAGAGCGACAACGGTCACCAAGAATAAAGCCGACGGCGCCGCCGCTCTGCTAAAAGCTTGCTCATCGCTCCGCCACCTGAAGCAAATCCAGGCCCACATTCTCCGCTCCAATCTCCACTGCAACGCCTCCTTAGCCTCCACTCTCATCTCTCTCTACGCCTCTCTCTCCTCCTCCTCCCCTCGCTACACCCACCATGTCTTCACCTCTTTCCCCAACCCAAACCTCTCTCTCTTCAACCACGCCATCAGGGCTTTCTCGAAGACCCACTCGCTTCGCTTCGAGGCGATGGTATTGTATAACCAAATGGTTCGGGTTGGAATAAGGCCTGATAACTATAGCTTCCCTTTTATGCTAAATTCGTGCGCCGCGAGGAGTGATCTTTGTGGAGGGGTGGAGGTCCATGGCCGCGTTGTTAAAACGGGGTTTGGGTTTTGTGTTCCGGTGTCGAATGCTCTGATTGATATGTATGGGAAATGTGGGGAGCTGGAGACCGCACGGAAGGTGTTTGATGAAATGCGTGAAAGAGATGTTGTTTCGTATAATGCGGTTTTAGGGGCACATGCCAGAGGAGGTGTGGATATGGAGAGTGCCGCTGATTTGTTCGAGGGAATGCAGGGGAGAAATGCTATATCTTGGAATGCGATGATTGTGGGGTATGTAAATAGTGGGGATGTGGTCTCGGCTAGGGATGTGTTTGATAGGATGCCGGAGAGGAATGTTGTTTCTTGGACTACAATGCTGGTGGGGTATACTAAAAATGGGTTTATGGATATGGCTAAGGATGTTTTTGATGAAATGCCTGAGCGAAGTTTGATTTGTTGGACGGCTGTGATATCAGGATACTCGCAAAATGGGAGGCCAAGTGAAGCACTTTCACTTTTCCGGAGAATGGAGAAATCAGGGGTAAAGCCGGATGCGTTTACCATGACAGCGTTGATCTCTGCATTGGCACAGTTGGGCCGTGCTGATCTGGCAAATTGGATTGGATCATATGTTGATCGAGCAGGCATAGAACAGAACGAACAAGTGCTCACTGCACTGGTTGACATGCATGCCAAGTGTGGGAATATGGAAGAAGCTTGTCGCGTTTTTGAGAAAATTCTTTGGAAGGATGCTTTCTCCTACAGTGCAATAATCACAGGTCTTGCTTCTCATGGTCATGGGATTAAAGCGCTTGAGATCTTCCGGAGGATGCTGGCAGAAAACATCGAACCTGATCACGTCACATTTGTTGGGGTATTGACTGCCTGTGGCCATGCAGGACTCGTCGAAGATGGAATGACCTATTGGGAAAGCATGATTCAAGATTACAATGTTCAGCCTGGTGCAGATCACTATGCTTGCGTGGTTGATATGCTGGGACGTGCTGGGAAGCTTAATGAGGCACACAATTTAGTCAAGGGCATGCCTATGGGTCCACAGCCTGGAGCATTGGGAGCTTTGCTTGCAGCATGTAGAACCTACGGAAATGTAGAGATTGCTGAAAGTATTGCTGAGAAGCTCTTCAAGTTAGAGCCTGAGAATACTGGAAATTATATGCTACTTTCTGGTATCTATGCATCAAAAGAGCGATGGGATGAAGCCAAAAAAGTGCGGGAAGCAATGAACAAGAAAAGTGGAATTAAGCTTCCTGGCTTTAGCTGGGTTGAAATTAGCAAAGGCCATTCAACTCAATTGAAGAACCAAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

565

Amino Acids

62.19

Weight (kDa)

7.6

Isoelectric Point (pI)

34.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 75 - 124 4.1e-06 PPR repeat family
PPR PF01535 152 - 178 1.7e-06 PPR repeat
PPR PF01535 212 - 242 2.1e-06 PPR repeat
PPR_2 PF13041 240 - 272 1.6e-06 PPR repeat family
PPR PF01535 243 - 271 8.5e-08 PPR repeat
PPR_2 PF13041 273 - 319 6.8e-14 PPR repeat family
PPR PF01535 275 - 304 1.3e-09 PPR repeat
PPR_long PF17177 275 - 369 9.2e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 373 - 420 6e-08 PPR repeat family
E_motif PF20431 490 - 552 7.4e-17 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016330)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g06640 FvH4_5g06640 FvH4_5g06640
malus_domestica MD06G1162200.v1.1 MD14G1167900.v1.1
prunus_persica Prupe.5G162800_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0192051
rosa_laevigata RLG00000004405
rosa_multiflora Rmu_ssc0000421.1_g000037
rosa_roxburghii Rroxscaffold_3G00263330
rosa_rugosa Rorug06G0512800.1
rosa_samantha Rh7BG124700 Rh7CG127400 Rh7DG126200
rosa_wichuraiana Rw7G010480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 41
AccB7I CCANNNNNTGG 1 cut(s) 1430
AccBSI CCGCTC 2 cut(s) 50, 111
AccI GTMKAC 1 cut(s) 755
AccII CGCG 3 cut(s) 374, 414, 1092
AccIII TCCGGA 2 cut(s) 888, 1191
AclWI GGATC 1 cut(s) 1000
AcoI YGGCCR 2 cut(s) 409, 1258
AcsI RAATTY 3 cut(s) 361, 1104, 1692
AcuI CTGAAG 1 cut(s) 104
AcyI GRCGYC 1 cut(s) 42
AfeI AGCGCT 1 cut(s) 1179
AfiI CCNNNNNNNGG 3 cut(s) 895, 1068, 1430
AgsI TTSAA 6 cut(s) 241, 1310, 1513, 1655, 1674, 1684
AjiI CACGTC 2 cut(s) 1228, 1370
AjnI CCWGG 4 cut(s) 93, 1327, 1429, 1636
AjuI GAANNNNNNNTTGG 2 cut(s) 1276, 1308
AluBI AGCT 9 cut(s) 62, 345, 490, 1085, 1381, 1445, 1507, 1633, 1647
AluI AGCT 9 cut(s) 62, 345, 490, 1085, 1381, 1445, 1507, 1633, 1647
Alw21I GWGCWC 1 cut(s) 1038
Alw26I GTCTC 2 cut(s) 488, 688
AlwI GGATC 1 cut(s) 1000
Aor13HI TCCGGA 2 cut(s) 888, 1191
Aor51HI AGCGCT 1 cut(s) 1179
AoxI GGCC 7 cut(s) 96, 329, 409, 863, 964, 1258, 1666
ApeKI GCWGC 1 cut(s) 1454
ApoI RAATTY 3 cut(s) 361, 1104, 1692
Asp700I GAANNNNTTC 1 cut(s) 1508
AspLEI GCGC 3 cut(s) 44, 371, 1180
AspS9I GGNCC 4 cut(s) 97, 403, 964, 1421
AsuHPI GGTGA 2 cut(s) 13, 199
AvaII GGWCC 2 cut(s) 403, 1421
BaeGI GKGCMC 1 cut(s) 565
BalI TGGCCA 1 cut(s) 1260
BanI GGYRCC 1 cut(s) 41
BbsI GAAGAC 2 cut(s) 196, 272
Bbv12I GWGCWC 1 cut(s) 1038
BbvI GCAGC 1 cut(s) 1466
BccI CCATC 4 cut(s) 257, 286, 1274, 1572
BceAI ACGGC 3 cut(s) 55, 846, 951
BciT130I CCWGG 4 cut(s) 95, 1329, 1431, 1638
BciVI GTATCC 1 cut(s) 839
BclI TGATCA 2 cut(s) 3, 1222
BcoDI GTCTC 2 cut(s) 488, 688
BfaI CTAG 1 cut(s) 690
BfmI CTRYAG 2 cut(s) 340, 1129
BfoI RGCGCY 2 cut(s) 45, 1181
BfuI GTATCC 1 cut(s) 839
BglI GCCNNNNNGGC 1 cut(s) 254
BglII AGATCT 1 cut(s) 1185
BisI GCNGC 6 cut(s) 45, 48, 372, 412, 597, 1455
BlsI GCNGC 6 cut(s) 46, 49, 373, 413, 598, 1456
Bme1390I CCNGG 4 cut(s) 95, 1329, 1431, 1638
Bme18I GGWCC 2 cut(s) 403, 1421
BmgBI CACGTC 2 cut(s) 1228, 1370
BmgT120I GGNCC 4 cut(s) 97, 403, 964, 1421
BmiI GGNNCC 2 cut(s) 43, 1422
BmrFI CCNGG 4 cut(s) 95, 1329, 1431, 1638
BmsI GCATC 5 cut(s) 699, 910, 1108, 1188, 1574
BoxI GACNNNNGTC 1 cut(s) 17
BpiI GAAGAC 2 cut(s) 196, 272
BpmI CTGGAG 2 cut(s) 512, 1452
Bpu10I CCTNAGC 3 cut(s) 136, 783, 807
BpuEI CTTGAG 1 cut(s) 1202
BsaHI GRCGYC 1 cut(s) 42
BsaI GGTCTC 2 cut(s) 488, 688
BsaJI CCNNGG 1 cut(s) 406
BsaWI WCCGGW 3 cut(s) 445, 888, 1191
BsaXI ACNNNNNCTCC 2 cut(s) 853, 883
Bsc4I CCNNNNNNNGG 3 cut(s) 895, 1068, 1430
Bse1I ACTGG 2 cut(s) 1050, 1537
Bse3DI GCAATG 1 cut(s) 1614
BseAI TCCGGA 2 cut(s) 888, 1191
BseBI CCWGG 4 cut(s) 95, 1329, 1431, 1638
BseDI CCNNGG 1 cut(s) 406
BseGI GGATG 8 cut(s) 682, 700, 714, 793, 925, 1123, 1203, 1588
BseLI CCNNNNNNNGG 3 cut(s) 895, 1068, 1430
BseMI GCAATG 1 cut(s) 1614
BseMII CTCAG 3 cut(s) 798, 1491, 1515
BseNI ACTGG 2 cut(s) 1050, 1537
BseRI GAGGAG 5 cut(s) 163, 166, 169, 391, 588
BseSI GKGCMC 1 cut(s) 565
BseXI GCAGC 1 cut(s) 1466
BseYI CCCAGC 3 cut(s) 1363, 1373, 1647
BsgI GTGCAG 2 cut(s) 1026, 1353
Bsh1236I CGCG 3 cut(s) 374, 414, 1092
BshFI GGCC 7 cut(s) 98, 331, 411, 865, 966, 1260, 1668
BshNI GGYRCC 1 cut(s) 41
BsiHKAI GWGCWC 1 cut(s) 1038
BsiSI CCGG 5 cut(s) 446, 713, 889, 917, 1192
BslFI GGGAC 1 cut(s) 1380
BslI CCNNNNNNNGG 3 cut(s) 895, 1068, 1430
BsmAI GTCTC 2 cut(s) 488, 688
BsmFI GGGAC 1 cut(s) 1380
BsmI GAATGC 3 cut(s) 460, 621, 649
BsnI GGCC 7 cut(s) 98, 331, 411, 865, 966, 1260, 1668
Bso31I GGTCTC 2 cut(s) 488, 688
Bsp1286I GDGCHC 2 cut(s) 565, 1038
Bsp13I TCCGGA 2 cut(s) 888, 1191
Bsp143I GATC 9 cut(s) 3, 382, 942, 973, 992, 1003, 1185, 1222, 1336
Bsp19I CCATGG 1 cut(s) 406
BspANI GGCC 7 cut(s) 98, 331, 411, 865, 966, 1260, 1668
BspCNI CTCAG 3 cut(s) 799, 1492, 1516
BspEI TCCGGA 2 cut(s) 888, 1191
BspFNI CGCG 3 cut(s) 374, 414, 1092
BspLI GGNNCC 2 cut(s) 43, 1422
BspPI GGATC 1 cut(s) 1000
BspQI GCTCTTC 1 cut(s) 1514
BspT107I GGYRCC 1 cut(s) 41
BspTNI GGTCTC 2 cut(s) 488, 688
BsrBI CCGCTC 2 cut(s) 50, 111
BsrDI GCAATG 1 cut(s) 1614
BsrI ACTGG 2 cut(s) 1050, 1537
BssECI CCNNGG 1 cut(s) 406
BssMI GATC 9 cut(s) 3, 382, 942, 973, 992, 1003, 1185, 1222, 1336
BssNAI GTATAC 1 cut(s) 756
BssNI GRCGYC 1 cut(s) 42
BssT1I CCWWGG 1 cut(s) 406
Bst1107I GTATAC 1 cut(s) 756
Bst2UI CCWGG 4 cut(s) 95, 1329, 1431, 1638
Bst4CI ACNGT 3 cut(s) 19, 960, 1133
Bst6I CTCTTC 2 cut(s) 242, 1514
BstACI GRCGYC 1 cut(s) 42
BstC8I GCNNGC 7 cut(s) 64, 1012, 1059, 1203, 1348, 1412, 1452
BstDEI CTNAG 5 cut(s) 136, 783, 807, 1500, 1524
BstDSI CCRYGG 1 cut(s) 406
BstEII GGTNACC 1 cut(s) 19
BstF5I GGATG 8 cut(s) 682, 700, 714, 793, 925, 1123, 1203, 1588
BstFNI CGCG 3 cut(s) 374, 414, 1092
BstH2I RGCGCY 2 cut(s) 45, 1181
BstHHI GCGC 3 cut(s) 44, 371, 1180
BstKTI GATC 9 cut(s) 6, 385, 945, 976, 995, 1006, 1188, 1225, 1339
BstMAI GTCTC 2 cut(s) 488, 688
BstMBI GATC 9 cut(s) 3, 382, 942, 973, 992, 1003, 1185, 1222, 1336
BstMWI GCNNNNNNNGC 6 cut(s) 41, 137, 254, 977, 1442, 1451
BstNI CCWGG 4 cut(s) 95, 1329, 1431, 1638
BstNSI RCATGY 5 cut(s) 569, 1057, 1061, 1414, 1461
BstPAI GACNNNNGTC 1 cut(s) 17
BstPI GGTNACC 1 cut(s) 19
BstSCI CCNGG 4 cut(s) 93, 1327, 1429, 1636
BstSFI CTRYAG 2 cut(s) 340, 1129
BstSLI GKGCMC 1 cut(s) 565
BstUI CGCG 3 cut(s) 374, 414, 1092
BstV1I GCAGC 1 cut(s) 1466
BstV2I GAAGAC 2 cut(s) 196, 272
BstX2I RGATCY 1 cut(s) 1185
BstYI RGATCY 1 cut(s) 1185
BstZ17I GTATAC 1 cut(s) 756
BsuI GTATCC 1 cut(s) 839
BsuRI GGCC 7 cut(s) 98, 331, 411, 865, 966, 1260, 1668
BtgI CCRYGG 1 cut(s) 406
BtgZI GCGATG 4 cut(s) 54, 305, 662, 1591
BtrI CACGTC 2 cut(s) 1228, 1370
BtsCI GGATG 8 cut(s) 682, 700, 714, 793, 925, 1123, 1203, 1588
BtsI GCAGTG 2 cut(s) 121, 1038
BtsIMutI CAGTG 4 cut(s) 121, 1038, 1043, 1138
Cac8I GCNNGC 7 cut(s) 64, 1012, 1059, 1203, 1348, 1412, 1452
CfoI GCGC 3 cut(s) 44, 371, 1180
Cfr13I GGNCC 4 cut(s) 97, 403, 964, 1421
CspCI CAANNNNNGTGG 4 cut(s) 729, 764, 1603, 1638
DdeI CTNAG 5 cut(s) 136, 783, 807, 1500, 1524
DinI GGCGCC 1 cut(s) 43
DpnI GATC 9 cut(s) 5, 384, 944, 975, 994, 1005, 1187, 1224, 1338
DpnII GATC 9 cut(s) 3, 382, 942, 973, 992, 1003, 1185, 1222, 1336
EaeI YGGCCR 2 cut(s) 409, 1258
Eam1104I CTCTTC 2 cut(s) 242, 1514
EarI CTCTTC 2 cut(s) 242, 1514
EciI GGCGGA 1 cut(s) 65
Eco130I CCWWGG 1 cut(s) 406
Eco147I AGGCCT 1 cut(s) 331
Eco31I GGTCTC 2 cut(s) 488, 688
Eco32I GATATC 1 cut(s) 840
Eco47I GGWCC 2 cut(s) 403, 1421
Eco47III AGCGCT 1 cut(s) 1179
Eco57I CTGAAG 1 cut(s) 104
Eco91I GGTNACC 1 cut(s) 19
EcoO65I GGTNACC 1 cut(s) 19
EcoRII CCWGG 4 cut(s) 93, 1327, 1429, 1636
EcoRV GATATC 1 cut(s) 840
EcoT14I CCWWGG 1 cut(s) 406
EcoT22I ATGCAT 2 cut(s) 1059, 1567
EgeI GGCGCC 1 cut(s) 43
EheI GGCGCC 1 cut(s) 43
ErhI CCWWGG 1 cut(s) 406
FaqI GGGAC 1 cut(s) 1380
FauI CCCGC 1 cut(s) 1593
FauNDI CATATG 1 cut(s) 997
FbaI TGATCA 2 cut(s) 3, 1222
FblI GTMKAC 1 cut(s) 755
Fnu4HI GCNGC 6 cut(s) 45, 48, 372, 412, 597, 1455
FokI GGATG 8 cut(s) 689, 707, 721, 800, 932, 1130, 1210, 1595
Fsp4HI GCNGC 6 cut(s) 45, 48, 372, 412, 597, 1455
FspBI CTAG 1 cut(s) 690
GlaI GCGC 3 cut(s) 43, 370, 1179
GluI GCNGC 6 cut(s) 45, 48, 372, 412, 597, 1455
GsaI CCCAGC 3 cut(s) 1367, 1377, 1651
GsuI CTGGAG 2 cut(s) 512, 1452
HaeII RGCGCY 2 cut(s) 45, 1181
HaeIII GGCC 7 cut(s) 98, 331, 411, 865, 966, 1260, 1668
HapII CCGG 5 cut(s) 446, 713, 889, 917, 1192
HhaI GCGC 3 cut(s) 44, 371, 1180
Hin1I GRCGYC 1 cut(s) 42
Hin6I GCGC 3 cut(s) 42, 369, 1178
HinP1I GCGC 3 cut(s) 42, 369, 1178
HincII GTYRAC 1 cut(s) 1051
HindII GTYRAC 1 cut(s) 1051
HindIII AAGCTT 4 cut(s) 60, 1083, 1379, 1631
HinfI GANTC 2 cut(s) 1269, 1306
HpaII CCGG 5 cut(s) 446, 713, 889, 917, 1192
HphI GGTGA 2 cut(s) 13, 199
Hpy166II GTNNAC 4 cut(s) 756, 927, 1051, 1424
Hpy188I TCNGA 2 cut(s) 8, 462
Hpy188III TCNNGA 5 cut(s) 262, 843, 889, 1192, 1310
Hpy8I GTNNAC 4 cut(s) 756, 927, 1051, 1424
Hpy99I CGWCG 2 cut(s) 41, 1277
HpyAV CCTTC 2 cut(s) 499, 1108
HpyCH4III ACNGT 3 cut(s) 19, 960, 1133
HpyCH4IV ACGT 2 cut(s) 1227, 1369
HpyF10VI GCNNNNNNNGC 6 cut(s) 41, 137, 254, 977, 1442, 1451
HpyF3I CTNAG 5 cut(s) 136, 783, 807, 1500, 1524
HpySE526I ACGT 2 cut(s) 1227, 1369
Hsp92I GRCGYC 1 cut(s) 42
HspAI GCGC 3 cut(s) 42, 369, 1178
KasI GGCGCC 1 cut(s) 41
Kpn2I TCCGGA 2 cut(s) 888, 1191
Ksp22I TGATCA 2 cut(s) 3, 1222
Kzo9I GATC 9 cut(s) 3, 382, 942, 973, 992, 1003, 1185, 1222, 1336
LguI GCTCTTC 1 cut(s) 1514
LmnI GCTCC 5 cut(s) 78, 116, 487, 1433, 1442
Lsp1109I GCAGC 1 cut(s) 1466
LweI GCATC 5 cut(s) 699, 910, 1108, 1188, 1574
MaeI CTAG 1 cut(s) 690
MaeII ACGT 2 cut(s) 1227, 1369
MaeIII GTNAC 2 cut(s) 19, 1228
MalI GATC 9 cut(s) 5, 384, 944, 975, 994, 1005, 1187, 1224, 1338
MbiI CCGCTC 2 cut(s) 50, 111
MboI GATC 9 cut(s) 3, 382, 942, 973, 992, 1003, 1185, 1222, 1336
MboII GAAGA 8 cut(s) 196, 229, 277, 1091, 1180, 1289, 1501, 1696
MfeI CAATTG 1 cut(s) 1679
MflI RGATCY 1 cut(s) 1185
MhlI GDGCHC 2 cut(s) 565, 1038
MlsI TGGCCA 1 cut(s) 1260
MluCI AATT 9 cut(s) 361, 982, 1104, 1396, 1537, 1626, 1656, 1679, 1692
MluNI TGGCCA 1 cut(s) 1260
Mly113I GGCGCC 1 cut(s) 42
MlyI GAGTC 1 cut(s) 1263
MmeI TCCRAC 2 cut(s) 301, 806
Mox20I TGGCCA 1 cut(s) 1260
Mph1103I ATGCAT 2 cut(s) 1059, 1567
MroI TCCGGA 2 cut(s) 888, 1191
MroXI GAANNNNTTC 1 cut(s) 1508
MscI TGGCCA 1 cut(s) 1260
MseI TTAA 5 cut(s) 420, 1173, 1383, 1629, 1696
MslI CAYNNNNRTG 2 cut(s) 1164, 1415
Msp20I TGGCCA 1 cut(s) 1260
MspA1I CMGCKG 1 cut(s) 599
MspI CCGG 5 cut(s) 446, 713, 889, 917, 1192
MspR9I CCNGG 4 cut(s) 95, 1329, 1431, 1638
MunI CAATTG 1 cut(s) 1679
Mva1269I GAATGC 3 cut(s) 460, 621, 649
MvaI CCWGG 4 cut(s) 95, 1329, 1431, 1638
MvnI CGCG 3 cut(s) 374, 414, 1092
MwoI GCNNNNNNNGC 6 cut(s) 41, 137, 254, 977, 1442, 1451
NarI GGCGCC 1 cut(s) 42
NcoI CCATGG 1 cut(s) 406
NdeI CATATG 1 cut(s) 997
NdeII GATC 9 cut(s) 3, 382, 942, 973, 992, 1003, 1185, 1222, 1336
NlaIV GGNNCC 2 cut(s) 43, 1422
NmeAIII GCCGAG 1 cut(s) 665
NmuCI GTSAC 2 cut(s) 19, 1228
NsiI ATGCAT 2 cut(s) 1059, 1567
NspI RCATGY 5 cut(s) 569, 1057, 1061, 1414, 1461
PaeI GCATGC 2 cut(s) 1061, 1414
PceI AGGCCT 1 cut(s) 331
PciSI GCTCTTC 1 cut(s) 1514
PcsI WCGNNNNNNNCGW 1 cut(s) 371
PctI GAATGC 3 cut(s) 460, 621, 649
PdmI GAANNNNTTC 1 cut(s) 1508
PfeI GAWTC 1 cut(s) 1306
PflMI CCANNNNNTGG 1 cut(s) 1430
PkrI GCNGC 6 cut(s) 46, 49, 373, 413, 598, 1456
PleI GAGTC 1 cut(s) 1263
PluTI GGCGCC 1 cut(s) 45
PpsI GAGTC 1 cut(s) 1263
PshAI GACNNNNGTC 1 cut(s) 17
Psp6I CCWGG 4 cut(s) 93, 1327, 1429, 1636
PspEI GGTNACC 1 cut(s) 19
PspFI CCCAGC 3 cut(s) 1363, 1373, 1647
PspGI CCWGG 4 cut(s) 93, 1327, 1429, 1636
PspN4I GGNNCC 2 cut(s) 43, 1422
PspPI GGNCC 4 cut(s) 97, 403, 964, 1421
PsuI RGATCY 1 cut(s) 1185
RseI CAYNNNNRTG 2 cut(s) 1164, 1415
SapI GCTCTTC 1 cut(s) 1514
SaqAI TTAA 5 cut(s) 420, 1173, 1383, 1629, 1696
SatI GCNGC 6 cut(s) 45, 48, 372, 412, 597, 1455
Sau3AI GATC 9 cut(s) 3, 382, 942, 973, 992, 1003, 1185, 1222, 1336
Sau96I GGNCC 4 cut(s) 97, 403, 964, 1421
SchI GAGTC 1 cut(s) 1263
ScrFI CCNGG 4 cut(s) 95, 1329, 1431, 1638
SduI GDGCHC 2 cut(s) 565, 1038
SfaNI GCATC 5 cut(s) 699, 910, 1108, 1188, 1574
SfcI CTRYAG 2 cut(s) 340, 1129
SfoI GGCGCC 1 cut(s) 43
SinI GGWCC 2 cut(s) 403, 1421
SmiMI CAYNNNNRTG 2 cut(s) 1164, 1415
SmlI CTYRAG 1 cut(s) 1181
SmoI CTYRAG 1 cut(s) 1181
SphI GCATGC 2 cut(s) 1061, 1414
Sse9I AATT 9 cut(s) 361, 982, 1104, 1396, 1537, 1626, 1656, 1679, 1692
SseBI AGGCCT 1 cut(s) 331
SspDI GGCGCC 1 cut(s) 41
SspMI CTAG 1 cut(s) 690
StuI AGGCCT 1 cut(s) 331
StyD4I CCNGG 4 cut(s) 93, 1327, 1429, 1636
StyI CCWWGG 1 cut(s) 406
TaaI ACNGT 3 cut(s) 19, 960, 1133
TaiI ACGT 2 cut(s) 1230, 1372
TaqI TCGA 7 cut(s) 263, 285, 452, 609, 1006, 1215, 1275
TasI AATT 9 cut(s) 361, 982, 1104, 1396, 1537, 1626, 1656, 1679, 1692
TauI GCSGC 5 cut(s) 47, 50, 374, 414, 599
TfiI GAWTC 1 cut(s) 1306
Tru1I TTAA 5 cut(s) 420, 1173, 1383, 1629, 1696
Tru9I TTAA 5 cut(s) 420, 1173, 1383, 1629, 1696
TscAI CASTG 4 cut(s) 128, 1045, 1050, 1138
TseFI GTSAC 2 cut(s) 19, 1228
TseI GCWGC 1 cut(s) 1454
Tsp45I GTSAC 2 cut(s) 19, 1228
TspDTI ATGAA 4 cut(s) 531, 813, 1599, 1625
TspGWI ACGGA 2 cut(s) 517, 1485
TspRI CASTG 4 cut(s) 128, 1045, 1050, 1138
Van91I CCANNNNNTGG 1 cut(s) 1430
VpaK11BI GGWCC 2 cut(s) 403, 1421
XapI RAATTY 3 cut(s) 361, 1104, 1692
XceI RCATGY 5 cut(s) 569, 1057, 1061, 1414, 1461
XcmI CCANNNNNNNNNTGG 1 cut(s) 577
XmiI GTMKAC 1 cut(s) 755
XmnI GAANNNNTTC 1 cut(s) 1508
XspI CTAG 1 cut(s) 690
Zsp2I ATGCAT 2 cut(s) 1059, 1567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.