Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0005872

Physical Location & Seq

Genomic Coordinates Reverse (-)

27779083 .. 27783752

Gene Structure 4670 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1185600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1734 bp

ATGTTGCCGCGCAAATGCGCGACATGGGTTCTTCTCTTCTTTCTGATCTCTCCCACCCTCATAAACTTATCGACCTCCGCATCGCCGTCCGATCCCCGTTGGGCAGCTAAGAAGCGACGCCTCAGAGATAAAGTCCGTAAGATGTTTTATCATGCATACGACAACTACATGATACATGCATTTCCGCATGATGAGCTAAAGCCACTGAGTAAAAGTTTCACTGACTCTCTTAGTGAACTTGGAAATCTGAAGCTTGAACACCTACCACAAGGCTACAGTGGATCGGCTCTGAGTCTTATAGAATCATTGTCCAGCCTTGTCATTATGGGAAACAACACTGAATTTGAAAGCGCAATTATTTGGCTTGCTGACAATTTGAAGTTCAATGTTGATGTGAGGGTAAATCTTTTTGAGTGCAACATAAGAGTGCTTGGAGGACTTGTTTCTGCTCATTTACTTGCAAGTGATTCTTCAAACAGGCTGGGTCAAGGAGTTTACAAGAATCAGCTGCTTACTTTGGCCGAGGACTTAGGGAAACGCTTCCTACCTGCATTTAACACGCCGACGGGGTTGCCGTATGCATGGATTAACTTAAAGTATGGAGTGATGGAGAATGAGACTACTGAAACAAGCACTTCAGGATGTGGTTCCCTAATTCTGGAAATGGGAGCATTGTCCCGATTGACTGGAGACCCCAAATACGAATCTGCAGCTTTACGTGCTCTTCGTAAATTATGGGGCATGCGTAGTTCCTTGAATCTACTTGGTACAACGCTGGATGTATCAACTGGGGAATGGCTTGAGTACTCATCTGGGATTGGAGCTGGGGTTGATTCATTTTATGAGTATCTATTTAAGGCCCACATACTTTTCGGAAAGGAGGAGTTCTGGAGAATGTTTCATTCTGCTTATATTGCTGTGCAGAAATATTTCAGACATGGTCCATGGTACCATGAAGCTGATATGAGGACAGGAAAAGCAACTTATTGGCAGCTTACTAGCCTTCAGGCATTTTGGCCTGGCCTACAGGCTGGGAATCTTAAACTTTCCGCAAATTCTTCACACCGTGAATTTTTCCATGTATGGGAAAAGTTTGGAGTAATACCAGAAAGGTATTTGTTGGACCATCAAATGTTGCATCCCACAGAAAAGTATTATCCTCTGCGCCCTGAATTGGCTGAGTCAACGTTCTACTTGTATCAAGCAACTAAAGACCCATGGTACATTGAAGTGGGGGAGTCAATTGTAAATTCTCTGAACTTATACACAAAAGTGGAAGGAGGTTTTGCTAGCATTAGAGATGTCACAACTATGCAGTTGGAAGATCATCAGCATAGTTTCTTCCTTGCTGAGACGTGCAAGTATCTATTTCTTTTGTTCGACGATTCATTTTTGGTTGAACAAAACTATATTTTCACAACCGAGGGTCACCCCCTTCCAGTGTTGAGTAGTTGGCATGACAGGCTTCCTGAGACTTACATCCATAGTAACCGGACTTCTCTCAAGGCCGGAAATCAAGCACGACGGACAAGTGCAATGTCTATGCAAGTATGTCCTACGACGATGAATTCGTGGGAATGCGGCGGCCAACAAGCTGAGAGTGCTTGCCACGTCCCAGATGCTCGTAGTGATCACAGGTGTCTAACAGACGATGAATGTGGAGTTGACTCAACTAATTGTAGACGAAGATCGTGTAGCATCGCTGGCTACTGCGGGCTATGGTTGTTCATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0003674 GO:0003824 GO:0004553 GO:0004559 GO:0004571 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0006491 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009100 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0012505 GO:0015923 GO:0015924 GO:0016020 GO:0016787 GO:0016798 GO:0019538 GO:0019941 GO:0030163 GO:0030433 GO:0031090 GO:0031984 GO:0033554 GO:0034976 GO:0036503 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051603 GO:0051716 GO:0071704 GO:0098588 GO:0098791 GO:1901135 GO:1901564 GO:1901565 GO:1901575 GO:1901698

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

Glyco_hydro_47

Protein Analysis

578

Amino Acids

65.64

Weight (kDa)

6.38

Isoelectric Point (pI)

43.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_47	PF01532	48 - 481	1.9e-112	Glycosyl hydrolase family 47

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005872)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G27520
fragaria_vesca	FvH4_5g16460 FvH4_5g16460 FvH4_5g16460 FvH4_5g16460 FvH4_5g16460 FvH4_5g16460 FvH4_5g16460 FvH4_5g16460
malus_domestica	MD14G1185600.v1.1
prunus_persica	Prupe.5G178500_v2.0.a1 Prupe.5G178500_v2.0.a1 Prupe.5G178500_v2.0.a1 Prupe.5G178500_v2.0.a1 Prupe.5G178500_v2.0.a1 Prupe.5G178500_v2.0.a1
pyrus_communis	pycom14g15450
rosa_chinensis	RchiOBHm_Chr7g0179631
rosa_laevigata	RLG00000005335
rosa_multiflora	Rmu_sc0005329.1_g000027
rosa_roxburghii	Rroxscaffold_3G00222510 Rroxscaffold_3G00273580
rosa_rugosa	Rorug06G0426800
rosa_samantha	Rh7AG026300 Rh7BG025400 Rh7CG027500 Rh7DG027100
rosa_wichuraiana	Rw7G002110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	556
Acc65I	GGTACC	1 cut(s)	948
AccB1I	GGYRCC	1 cut(s)	948
AccI	GTMKAC	1 cut(s)	1681
AccII	CGCG	2 cut(s)	10, 20
AciI	CCGC	7 cut(s)	8, 78, 185, 1050, 1581, 1584, 1713
AclI	AACGTT	1 cut(s)	1187
AclWI	GGATC	2 cut(s)	86, 289
AcoI	YGGCCR	2 cut(s)	519, 1585
AcsI	RAATTY	5 cut(s)	341, 1054, 1070, 1249, 1567
AcuI	CTGAAG	3 cut(s)	269, 621, 989
AcyI	GRCGYC	1 cut(s)	118
AdeI	CACNNNGTG	1 cut(s)	1067
AfaI	GTAC	4 cut(s)	769, 806, 950, 1223
AfiI	CCNNNNNNNGG	3 cut(s)	658, 1084, 1174
AgsI	TTSAA	8 cut(s)	257, 347, 379, 385, 474, 757, 1229, 1400
AjiI	CACGTC	2 cut(s)	1356, 1612
AjnI	CCWGG	1 cut(s)	1018
AleI	CACNNNNGTG	1 cut(s)	1271
AluBI	AGCT	9 cut(s)	107, 196, 253, 508, 713, 824, 959, 994, 1595
AluI	AGCT	9 cut(s)	107, 196, 253, 508, 713, 824, 959, 994, 1595
Alw21I	GWGCWC	1 cut(s)	724
Alw26I	GTCTC	4 cut(s)	611, 684, 1346, 1466
AlwI	GGATC	2 cut(s)	86, 289
AoxI	GGCC	6 cut(s)	519, 858, 1016, 1021, 1506, 1585
ApeKI	GCWGC	4 cut(s)	104, 508, 710, 991
ApoI	RAATTY	5 cut(s)	341, 1054, 1070, 1249, 1567
ArsI	GACNNNNNNTTYG	2 cut(s)	1552, 1584
Asp700I	GAANNNNTTC	2 cut(s)	539, 929
Asp718I	GGTACC	1 cut(s)	948
AspLEI	GCGC	4 cut(s)	12, 20, 353, 1167
AspS9I	GGNCC	3 cut(s)	859, 941, 1123
AsuHPI	GGTGA	1 cut(s)	1421
AsuNHI	GCTAGC	1 cut(s)	1289
AvaII	GGWCC	2 cut(s)	941, 1123
BanI	GGYRCC	1 cut(s)	948
BarI	GAAGNNNNNNTAC	2 cut(s)	1480, 1512
Bbv12I	GWGCWC	1 cut(s)	724
BbvI	GCAGC	4 cut(s)	116, 495, 722, 1003
BccI	CCATC	2 cut(s)	601, 1134
BceAI	ACGGC	2 cut(s)	70, 559
BcgI	CGANNNNNNTGC	2 cut(s)	553, 587
BciT130I	CCWGG	1 cut(s)	1020
BclI	TGATCA	1 cut(s)	1630
BcoDI	GTCTC	4 cut(s)	611, 684, 1346, 1466
BfaI	CTAG	2 cut(s)	999, 1290
BfmI	CTRYAG	3 cut(s)	274, 708, 1025
BfuAI	ACCTGC	1 cut(s)	556
BisI	GCNGC	7 cut(s)	8, 105, 509, 711, 992, 1582, 1585
BlsI	GCNGC	7 cut(s)	9, 106, 510, 712, 993, 1583, 1586
BmcAI	AGTACT	1 cut(s)	806
Bme1390I	CCNGG	1 cut(s)	1020
Bme18I	GGWCC	2 cut(s)	941, 1123
BmgBI	CACGTC	2 cut(s)	1356, 1612
BmgT120I	GGNCC	3 cut(s)	859, 941, 1123
BmiI	GGNNCC	2 cut(s)	649, 950
BmrFI	CCNGG	1 cut(s)	1020
BmrI	ACTGGG	1 cut(s)	798
BmsI	GCATC	4 cut(s)	89, 1147, 1609, 1707
BmtI	GCTAGC	1 cut(s)	1293
BmuI	ACTGGG	1 cut(s)	798
BpmI	CTGGAG	2 cut(s)	708, 910
BpuEI	CTTGAG	2 cut(s)	821, 1487
BsaAI	YACGTR	1 cut(s)	719
BsaHI	GRCGYC	1 cut(s)	118
BsaI	GGTCTC	1 cut(s)	684
BsaJI	CCNNGG	4 cut(s)	522, 944, 1217, 1422
BsaWI	WCCGGW	1 cut(s)	1491
BsaXI	ACNNNNNCTCC	4 cut(s)	426, 456, 813, 843
Bsc4I	CCNNNNNNNGG	3 cut(s)	658, 1084, 1174
Bse1I	ACTGG	3 cut(s)	691, 793, 1439
Bse3DI	GCAATG	1 cut(s)	1542
BseBI	CCWGG	1 cut(s)	1020
BseDI	CCNNGG	4 cut(s)	522, 944, 1217, 1422
BseGI	GGATG	4 cut(s)	647, 784, 1138, 1479
BseLI	CCNNNNNNNGG	3 cut(s)	658, 1084, 1174
BseMI	GCAATG	1 cut(s)	1542
BseMII	CTCAG	7 cut(s)	136, 197, 281, 1170, 1341, 1461, 1587
BseNI	ACTGG	3 cut(s)	691, 793, 1439
BseRI	GAGGAG	1 cut(s)	896
BseXI	GCAGC	4 cut(s)	116, 495, 722, 1003
BseYI	CCCAGC	3 cut(s)	481, 824, 1031
BsgI	GTGCAG	1 cut(s)	941
Bsh1236I	CGCG	2 cut(s)	10, 20
BshFI	GGCC	6 cut(s)	521, 860, 1018, 1023, 1508, 1587
BshNI	GGYRCC	1 cut(s)	948
BsiHKAI	GWGCWC	1 cut(s)	724
BsiSI	CCGG	2 cut(s)	1492, 1509
BslFI	GGGAC	2 cut(s)	661, 1598
BslI	CCNNNNNNNGG	3 cut(s)	658, 1084, 1174
BsmAI	GTCTC	4 cut(s)	611, 684, 1346, 1466
BsmBI	CGTCTC	1 cut(s)	1346
BsmFI	GGGAC	2 cut(s)	661, 1598
BsmI	GAATGC	1 cut(s)	1583
BsnI	GGCC	6 cut(s)	521, 860, 1018, 1023, 1508, 1587
Bso31I	GGTCTC	1 cut(s)	684
Bsp1286I	GDGCHC	1 cut(s)	724
Bsp143I	GATC	6 cut(s)	45, 91, 281, 1324, 1630, 1688
Bsp19I	CCATGG	2 cut(s)	944, 1217
BspACI	CCGC	7 cut(s)	8, 78, 185, 1050, 1581, 1584, 1713
BspANI	GGCC	6 cut(s)	521, 860, 1018, 1023, 1508, 1587
BspCNI	CTCAG	7 cut(s)	135, 198, 282, 1171, 1342, 1462, 1588
BspFNI	CGCG	2 cut(s)	10, 20
BspLI	GGNNCC	2 cut(s)	649, 950
BspMAI	CTGCAG	1 cut(s)	712
BspMI	ACCTGC	1 cut(s)	556
BspOI	GCTAGC	1 cut(s)	1293
BspPI	GGATC	2 cut(s)	86, 289
BspQI	GCTCTTC	1 cut(s)	729
BspT107I	GGYRCC	1 cut(s)	948
BspTNI	GGTCTC	1 cut(s)	684
BsrDI	GCAATG	1 cut(s)	1542
BsrI	ACTGG	3 cut(s)	691, 793, 1439
BssECI	CCNNGG	4 cut(s)	522, 944, 1217, 1422
BssMI	GATC	6 cut(s)	45, 91, 281, 1324, 1630, 1688
BssNI	GRCGYC	1 cut(s)	118
BssT1I	CCWWGG	2 cut(s)	944, 1217
Bst2UI	CCWGG	1 cut(s)	1020
Bst4CI	ACNGT	2 cut(s)	278, 1067
Bst6I	CTCTTC	2 cut(s)	41, 729
BstACI	GRCGYC	1 cut(s)	118
BstBAI	YACGTR	1 cut(s)	719
BstC8I	GCNNGC	6 cut(s)	366, 743, 1291, 1606, 1705, 1715
BstDSI	CCRYGG	2 cut(s)	944, 1217
BstEII	GGTNACC	1 cut(s)	1427
BstF5I	GGATG	4 cut(s)	647, 784, 1138, 1479
BstFNI	CGCG	2 cut(s)	10, 20
BstHHI	GCGC	4 cut(s)	12, 20, 353, 1167
BstKTI	GATC	6 cut(s)	48, 94, 284, 1327, 1633, 1691
BstMAI	GTCTC	4 cut(s)	611, 684, 1346, 1466
BstMBI	GATC	6 cut(s)	45, 91, 281, 1324, 1630, 1688
BstMWI	GCNNNNNNNGC	6 cut(s)	193, 719, 914, 1462, 1601, 1704
BstNI	CCWGG	1 cut(s)	1020
BstNSI	RCATGY	2 cut(s)	179, 745
BstPI	GGTNACC	1 cut(s)	1427
BstSCI	CCNGG	1 cut(s)	1018
BstSFI	CTRYAG	3 cut(s)	274, 708, 1025
BstUI	CGCG	2 cut(s)	10, 20
BstV1I	GCAGC	4 cut(s)	116, 495, 722, 1003
BsuRI	GGCC	6 cut(s)	521, 860, 1018, 1023, 1508, 1587
BtgI	CCRYGG	2 cut(s)	944, 1217
BtgZI	GCGATG	2 cut(s)	66, 1684
BtrI	CACGTC	2 cut(s)	1356, 1612
BtsCI	GGATG	4 cut(s)	647, 784, 1138, 1479
BtsIMutI	CAGTG	5 cut(s)	203, 219, 283, 336, 1446
BveI	ACCTGC	1 cut(s)	556
Cac8I	GCNNGC	6 cut(s)	366, 743, 1291, 1606, 1705, 1715
CfoI	GCGC	4 cut(s)	12, 20, 353, 1167
Cfr13I	GGNCC	3 cut(s)	859, 941, 1123
CseI	GACGC	1 cut(s)	126
Csp6I	GTAC	4 cut(s)	768, 805, 949, 1222
CviQI	GTAC	4 cut(s)	768, 805, 949, 1222
DpnI	GATC	6 cut(s)	47, 93, 283, 1326, 1632, 1690
DpnII	GATC	6 cut(s)	45, 91, 281, 1324, 1630, 1688
DraIII	CACNNNGTG	1 cut(s)	1067
EaeI	YGGCCR	2 cut(s)	519, 1585
Eam1104I	CTCTTC	2 cut(s)	41, 729
EarI	CTCTTC	2 cut(s)	41, 729
Eco130I	CCWWGG	2 cut(s)	944, 1217
Eco31I	GGTCTC	1 cut(s)	684
Eco47I	GGWCC	2 cut(s)	941, 1123
Eco57I	CTGAAG	3 cut(s)	269, 621, 989
Eco91I	GGTNACC	1 cut(s)	1427
EcoO65I	GGTNACC	1 cut(s)	1427
EcoRI	GAATTC	1 cut(s)	1567
EcoRII	CCWGG	1 cut(s)	1018
EcoT14I	CCWWGG	2 cut(s)	944, 1217
EcoT22I	ATGCAT	3 cut(s)	157, 181, 583
ErhI	CCWWGG	2 cut(s)	944, 1217
Esp3I	CGTCTC	1 cut(s)	1346
FalI	AAGNNNNNCTT	2 cut(s)	454, 486
FaqI	GGGAC	2 cut(s)	661, 1598
FauI	CCCGC	1 cut(s)	1706
FauNDI	CATATG	1 cut(s)	1730
FbaI	TGATCA	1 cut(s)	1630
FblI	GTMKAC	1 cut(s)	1681
Fnu4HI	GCNGC	7 cut(s)	8, 105, 509, 711, 992, 1582, 1585
FokI	GGATG	4 cut(s)	654, 791, 1125, 1466
Fsp4HI	GCNGC	7 cut(s)	8, 105, 509, 711, 992, 1582, 1585
FspBI	CTAG	2 cut(s)	999, 1290
GlaI	GCGC	4 cut(s)	11, 19, 352, 1166
GluI	GCNGC	7 cut(s)	8, 105, 509, 711, 992, 1582, 1585
GsaI	CCCAGC	3 cut(s)	485, 828, 1035
GsuI	CTGGAG	2 cut(s)	708, 910
HaeIII	GGCC	6 cut(s)	521, 860, 1018, 1023, 1508, 1587
HapII	CCGG	2 cut(s)	1492, 1509
HgaI	GACGC	1 cut(s)	126
HhaI	GCGC	4 cut(s)	12, 20, 353, 1167
Hin1I	GRCGYC	1 cut(s)	118
Hin6I	GCGC	4 cut(s)	10, 18, 351, 1165
HinP1I	GCGC	4 cut(s)	10, 18, 351, 1165
HincII	GTYRAC	2 cut(s)	1185, 1666
HindII	GTYRAC	2 cut(s)	1185, 1666
HindIII	AAGCTT	1 cut(s)	251
HpaII	CCGG	2 cut(s)	1492, 1509
HphI	GGTGA	1 cut(s)	1421
Hpy166II	GTNNAC	5 cut(s)	236, 496, 1185, 1666, 1682
Hpy188I	TCNGA	8 cut(s)	45, 91, 125, 249, 291, 875, 935, 1257
Hpy188III	TCNNGA	5 cut(s)	639, 659, 678, 889, 1469
Hpy8I	GTNNAC	5 cut(s)	236, 496, 1185, 1666, 1682
Hpy99I	CGWCG	5 cut(s)	120, 568, 1385, 1527, 1564
HpyAV	CCTTC	3 cut(s)	1013, 1271, 1445
HpyCH4III	ACNGT	2 cut(s)	278, 1067
HpyCH4IV	ACGT	4 cut(s)	718, 1187, 1355, 1611
HpyF10VI	GCNNNNNNNGC	6 cut(s)	193, 719, 914, 1462, 1601, 1704
HpySE526I	ACGT	4 cut(s)	718, 1187, 1355, 1611
Hsp92I	GRCGYC	1 cut(s)	118
HspAI	GCGC	4 cut(s)	10, 18, 351, 1165
KpnI	GGTACC	1 cut(s)	952
Ksp22I	TGATCA	1 cut(s)	1630
Kzo9I	GATC	6 cut(s)	45, 91, 281, 1324, 1630, 1688
LguI	GCTCTTC	1 cut(s)	729
LmnI	GCTCC	2 cut(s)	668, 821
Lsp1109I	GCAGC	4 cut(s)	116, 495, 722, 1003
LweI	GCATC	4 cut(s)	89, 1147, 1609, 1707
MaeI	CTAG	2 cut(s)	999, 1290
MaeII	ACGT	4 cut(s)	718, 1187, 1355, 1611
MaeIII	GTNAC	3 cut(s)	1303, 1427, 1487
MalI	GATC	6 cut(s)	47, 93, 283, 1326, 1632, 1690
MboI	GATC	6 cut(s)	45, 91, 281, 1324, 1630, 1688
MboII	GAAGA	8 cut(s)	23, 28, 462, 716, 1050, 1333, 1334, 1698
MfeI	CAATTG	1 cut(s)	1242
MhlI	GDGCHC	1 cut(s)	724
MlyI	GAGTC	5 cut(s)	218, 301, 1190, 1247, 1661
MmeI	TCCRAC	2 cut(s)	1101, 1299
Mph1103I	ATGCAT	3 cut(s)	157, 181, 583
MroXI	GAANNNNTTC	2 cut(s)	539, 929
MseI	TTAA	5 cut(s)	555, 588, 593, 855, 1041
MslI	CAYNNNNRTG	4 cut(s)	425, 1229, 1271, 1310
MspA1I	CMGCKG	1 cut(s)	508
MspI	CCGG	2 cut(s)	1492, 1509
MspR9I	CCNGG	1 cut(s)	1020
MunI	CAATTG	1 cut(s)	1242
Mva1269I	GAATGC	1 cut(s)	1583
MvaI	CCWGG	1 cut(s)	1020
MvnI	CGCG	2 cut(s)	10, 20
MwoI	GCNNNNNNNGC	6 cut(s)	193, 719, 914, 1462, 1601, 1704
NcoI	CCATGG	2 cut(s)	944, 1217
NdeI	CATATG	1 cut(s)	1730
NdeII	GATC	6 cut(s)	45, 91, 281, 1324, 1630, 1688
NheI	GCTAGC	1 cut(s)	1289
NlaIV	GGNNCC	2 cut(s)	649, 950
NmeAIII	GCCGAG	1 cut(s)	547
NmuCI	GTSAC	2 cut(s)	1303, 1427
NsiI	ATGCAT	3 cut(s)	157, 181, 583
NspI	RCATGY	2 cut(s)	179, 745
OliI	CACNNNNGTG	1 cut(s)	1271
PaeI	GCATGC	1 cut(s)	745
PciSI	GCTCTTC	1 cut(s)	729
PcsI	WCGNNNNNNNCGW	3 cut(s)	572, 724, 1568
PctI	GAATGC	1 cut(s)	1583
PdmI	GAANNNNTTC	2 cut(s)	539, 929
PfeI	GAWTC	8 cut(s)	302, 467, 502, 704, 757, 833, 1036, 1385
PflFI	GACNNNGTC	1 cut(s)	939
PkrI	GCNGC	7 cut(s)	9, 106, 510, 712, 993, 1583, 1586
PleI	GAGTC	5 cut(s)	218, 300, 1189, 1246, 1661
PpsI	GAGTC	5 cut(s)	218, 300, 1189, 1246, 1661
Ppu21I	YACGTR	1 cut(s)	719
Psp1406I	AACGTT	1 cut(s)	1187
Psp6I	CCWGG	1 cut(s)	1018
PspEI	GGTNACC	1 cut(s)	1427
PspFI	CCCAGC	3 cut(s)	481, 824, 1031
PspGI	CCWGG	1 cut(s)	1018
PspN4I	GGNNCC	2 cut(s)	649, 950
PspPI	GGNCC	3 cut(s)	859, 941, 1123
PstI	CTGCAG	1 cut(s)	712
PsyI	GACNNNGTC	1 cut(s)	939
PvuII	CAGCTG	1 cut(s)	508
RsaI	GTAC	4 cut(s)	769, 806, 950, 1223
RsaNI	GTAC	4 cut(s)	768, 805, 949, 1222
RseI	CAYNNNNRTG	4 cut(s)	425, 1229, 1271, 1310
SapI	GCTCTTC	1 cut(s)	729
SaqAI	TTAA	5 cut(s)	555, 588, 593, 855, 1041
SatI	GCNGC	7 cut(s)	8, 105, 509, 711, 992, 1582, 1585
Sau3AI	GATC	6 cut(s)	45, 91, 281, 1324, 1630, 1688
Sau96I	GGNCC	3 cut(s)	859, 941, 1123
ScaI	AGTACT	1 cut(s)	806
SchI	GAGTC	5 cut(s)	218, 301, 1190, 1247, 1661
ScrFI	CCNGG	1 cut(s)	1020
SduI	GDGCHC	1 cut(s)	724
SfaNI	GCATC	4 cut(s)	89, 1147, 1609, 1707
SfcI	CTRYAG	3 cut(s)	274, 708, 1025
SinI	GGWCC	2 cut(s)	941, 1123
SmiMI	CAYNNNNRTG	4 cut(s)	425, 1229, 1271, 1310
SmlI	CTYRAG	2 cut(s)	800, 1502
SmoI	CTYRAG	2 cut(s)	800, 1502
SphI	GCATGC	1 cut(s)	745
SsiI	CCGC	7 cut(s)	8, 78, 185, 1050, 1581, 1584, 1713
SspI	AATATT	1 cut(s)	929
SspMI	CTAG	2 cut(s)	999, 1290
StyD4I	CCNGG	1 cut(s)	1018
StyI	CCWWGG	2 cut(s)	944, 1217
TaaI	ACNGT	2 cut(s)	278, 1067
TaiI	ACGT	4 cut(s)	721, 1190, 1358, 1614
TaqI	TCGA	2 cut(s)	71, 1380
TatI	WGTACW	1 cut(s)	804
TauI	GCSGC	3 cut(s)	10, 1584, 1587
TfiI	GAWTC	8 cut(s)	302, 467, 502, 704, 757, 833, 1036, 1385
Tru1I	TTAA	5 cut(s)	555, 588, 593, 855, 1041
Tru9I	TTAA	5 cut(s)	555, 588, 593, 855, 1041
TscAI	CASTG	5 cut(s)	210, 226, 283, 343, 1446
TseFI	GTSAC	2 cut(s)	1303, 1427
TseI	GCWGC	4 cut(s)	104, 508, 710, 991
Tsp45I	GTSAC	2 cut(s)	1303, 1427
TspDTI	ATGAA	7 cut(s)	825, 890, 969, 1377, 1580, 1668, 1717
TspGWI	ACGGA	2 cut(s)	125, 1540
TspRI	CASTG	5 cut(s)	210, 226, 283, 343, 1446
Tth111I	GACNNNGTC	1 cut(s)	939
VpaK11BI	GGWCC	2 cut(s)	941, 1123
XapI	RAATTY	5 cut(s)	341, 1054, 1070, 1249, 1567
XceI	RCATGY	2 cut(s)	179, 745
XmiI	GTMKAC	1 cut(s)	1681
XmnI	GAANNNNTTC	2 cut(s)	539, 929
XspI	CTAG	2 cut(s)	999, 1290
ZrmI	AGTACT	1 cut(s)	806
Zsp2I	ATGCAT	3 cut(s)	157, 181, 583

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.