MD14G1204500.v1.1

transcription, DNA-templated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0015629
Physical Location & Seq
Forward (+)
29245311 .. 29246506
1196 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1204500.v1.1.491

Sequence Viewer

Length: 522 bp
ATGATCAGTCCTTTTTCTTCTGCATTTTTAATTCAAGTTTCGAATCAATTATTTATTTCTTTTGCGTTCCAAGCACCCTTGACAGTAATATCAGTTGCCTCGGCTGTGGAAGTGTTGGATGCGCTGAATGCCGAGTGGTCATTCATTGATATCATACTTGCTGCAATTGATCTTCCTATAGAGGCAAGCATGAGCATGCTCAACTATATAATGCAGGATCTGCATTTCAAACACATTCCAGTTATCATGCTGGCGACGCAGGATGAAAGCCGTTTCCTTTTCAATTTCTTAAAATTTGGGGCCACTGATTATCTGGTGAAGCCACTGTGCATTGATCAAATACTAAACTTGTGGATGCACACTTGGAGGCAGAGGAAGCAACCTAAGGGGCTGGAGGCGCCGCAGAAGAACATTGGTCTGGTGGTTTCGTCTGAAGCAACGATGAACAACATCTTGGTCTTGGATGACACAGAAGAAGAGTTTGCAGAGGATCCAAAGCCAGCTATGTGTATCAAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04712 map04712
Pfam Domains
Protein Families
CCT Response_reg

Protein Analysis

174

Amino Acids

19.57

Weight (kDa)

4.74

Isoelectric Point (pI)

49.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Response_reg PF00072 26 - 116 1.7e-08 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015629)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g14580 FvH4_5g14580
malus_domestica MD14G1204500.v1.1
prunus_persica Prupe.5G197800_v2.0.a1 Prupe.5G197800_v2.0.a1
pyrus_communis pycom14g16900
rosa_chinensis RchiOBHm_Chr7g0181971 RchiOBHm_Chr7g0181991
rosa_laevigata RLG00000005163
rosa_roxburghii Rroxscaffold_3G00271640
rosa_samantha Rh7AG047300 Rh7BG047200 Rh7CG047500 Rh7DG046500
rosa_wichuraiana Rw0G019680 Rw7G003920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 397
AciI CCGC 1 cut(s) 401
AclWI GGATC 3 cut(s) 225, 485, 498
AcsI RAATTY 1 cut(s) 293
AcuI CTGAAG 1 cut(s) 453
AcyI GRCGYC 1 cut(s) 398
AgsI TTSAA 3 cut(s) 35, 229, 283
AjuI GAANNNNNNNTTGG 2 cut(s) 437, 469
AluBI AGCT 1 cut(s) 503
AluI AGCT 1 cut(s) 503
AlwI GGATC 3 cut(s) 225, 485, 498
AlwNI CAGNNNCTG 1 cut(s) 220
AoxI GGCC 1 cut(s) 300
ApeKI GCWGC 1 cut(s) 161
ApoI RAATTY 1 cut(s) 293
AspLEI GCGC 2 cut(s) 124, 400
AspS9I GGNCC 1 cut(s) 300
AsuHPI GGTGA 1 cut(s) 328
AsuII TTCGAA 1 cut(s) 41
AxyI CCTNAGG 1 cut(s) 384
BamHI GGATCC 1 cut(s) 490
BanI GGYRCC 1 cut(s) 397
BbvI GCAGC 1 cut(s) 148
BceAI ACGGC 1 cut(s) 255
BclI TGATCA 2 cut(s) 3, 334
BfmI CTRYAG 1 cut(s) 177
BfoI RGCGCY 1 cut(s) 401
BisI GCNGC 2 cut(s) 162, 401
BlsI GCNGC 2 cut(s) 163, 402
BmgT120I GGNCC 1 cut(s) 300
BmiI GGNNCC 3 cut(s) 301, 399, 492
BmsI GCATC 2 cut(s) 109, 345
BpmI CTGGAG 1 cut(s) 413
Bpu14I TTCGAA 1 cut(s) 41
BsaHI GRCGYC 1 cut(s) 398
BsaJI CCNNGG 1 cut(s) 99
Bse1I ACTGG 1 cut(s) 239
Bse21I CCTNAGG 1 cut(s) 384
BseDI CCNNGG 1 cut(s) 99
BseGI GGATG 4 cut(s) 124, 268, 360, 469
BseNI ACTGG 1 cut(s) 239
BseXI GCAGC 1 cut(s) 148
BshFI GGCC 1 cut(s) 302
BshNI GGYRCC 1 cut(s) 397
BsmI GAATGC 1 cut(s) 133
BsnI GGCC 1 cut(s) 302
Bsp119I TTCGAA 1 cut(s) 41
Bsp143I GATC 5 cut(s) 3, 169, 217, 334, 490
BspACI CCGC 1 cut(s) 401
BspANI GGCC 1 cut(s) 302
BspLI GGNNCC 3 cut(s) 301, 399, 492
BspPI GGATC 3 cut(s) 225, 485, 498
BspT104I TTCGAA 1 cut(s) 41
BspT107I GGYRCC 1 cut(s) 397
BsrI ACTGG 1 cut(s) 239
BssECI CCNNGG 1 cut(s) 99
BssMI GATC 5 cut(s) 3, 169, 217, 334, 490
BssNI GRCGYC 1 cut(s) 398
Bst4CI ACNGT 2 cut(s) 85, 327
Bst6I CTCTTC 1 cut(s) 471
BstACI GRCGYC 1 cut(s) 398
BstAPI GCANNNNNTGC 1 cut(s) 220
BstBI TTCGAA 1 cut(s) 41
BstC8I GCNNGC 4 cut(s) 187, 197, 252, 501
BstDEI CTNAG 1 cut(s) 384
BstF5I GGATG 4 cut(s) 124, 268, 360, 469
BstH2I RGCGCY 1 cut(s) 401
BstHHI GCGC 2 cut(s) 124, 400
BstKTI GATC 5 cut(s) 6, 172, 220, 337, 493
BstMBI GATC 5 cut(s) 3, 169, 217, 334, 490
BstMWI GCNNNNNNNGC 6 cut(s) 71, 128, 220, 256, 376, 397
BstNSI RCATGY 1 cut(s) 199
BstSFI CTRYAG 1 cut(s) 177
BstV1I GCAGC 1 cut(s) 148
BstX2I RGATCY 2 cut(s) 217, 490
BstYI RGATCY 2 cut(s) 217, 490
Bsu36I CCTNAGG 1 cut(s) 384
BsuRI GGCC 1 cut(s) 302
BtsCI GGATG 4 cut(s) 124, 268, 360, 469
BtsIMutI CAGTG 2 cut(s) 303, 323
Cac8I GCNNGC 4 cut(s) 187, 197, 252, 501
CaiI CAGNNNCTG 1 cut(s) 220
CfoI GCGC 2 cut(s) 124, 400
Cfr13I GGNCC 1 cut(s) 300
CseI GACGC 1 cut(s) 265
CviAII CATG 3 cut(s) 190, 196, 247
CviJI RGCY 7 cut(s) 104, 270, 302, 322, 391, 499, 503
CviKI_1 RGCY 7 cut(s) 104, 270, 302, 322, 391, 499, 503
DdeI CTNAG 1 cut(s) 384
DinI GGCGCC 1 cut(s) 399
DpnI GATC 5 cut(s) 5, 171, 219, 336, 492
DpnII GATC 5 cut(s) 3, 169, 217, 334, 490
Eam1104I CTCTTC 1 cut(s) 471
EarI CTCTTC 1 cut(s) 471
Eco32I GATATC 1 cut(s) 151
Eco57I CTGAAG 1 cut(s) 453
Eco81I CCTNAGG 1 cut(s) 384
EcoRV GATATC 1 cut(s) 151
EgeI GGCGCC 1 cut(s) 399
EheI GGCGCC 1 cut(s) 399
FaeI CATG 3 cut(s) 193, 199, 250
FaiI YATR 8 cut(s) 155, 179, 191, 197, 207, 209, 248, 506
FatI CATG 3 cut(s) 189, 195, 246
FbaI TGATCA 2 cut(s) 3, 334
Fnu4HI GCNGC 2 cut(s) 162, 401
FokI GGATG 4 cut(s) 131, 275, 367, 476
Fsp4HI GCNGC 2 cut(s) 162, 401
GlaI GCGC 2 cut(s) 123, 399
GluI GCNGC 2 cut(s) 162, 401
GsuI CTGGAG 1 cut(s) 413
HaeII RGCGCY 1 cut(s) 401
HaeIII GGCC 1 cut(s) 302
HgaI GACGC 1 cut(s) 265
HhaI GCGC 2 cut(s) 124, 400
Hin1I GRCGYC 1 cut(s) 398
Hin1II CATG 3 cut(s) 193, 199, 250
Hin6I GCGC 2 cut(s) 122, 398
HinP1I GCGC 2 cut(s) 122, 398
HinfI GANTC 1 cut(s) 43
HphI GGTGA 1 cut(s) 328
Hpy188I TCNGA 1 cut(s) 433
Hpy99I CGWCG 1 cut(s) 259
HpyCH4III ACNGT 2 cut(s) 85, 327
HpyCH4V TGCA 7 cut(s) 23, 164, 214, 223, 330, 358, 485
HpyF10VI GCNNNNNNNGC 6 cut(s) 71, 128, 220, 256, 376, 397
HpyF3I CTNAG 1 cut(s) 384
Hsp92I GRCGYC 1 cut(s) 398
Hsp92II CATG 3 cut(s) 193, 199, 250
HspAI GCGC 2 cut(s) 122, 398
KasI GGCGCC 1 cut(s) 397
Ksp22I TGATCA 2 cut(s) 3, 334
Kzo9I GATC 5 cut(s) 3, 169, 217, 334, 490
LpnPI CCDG 8 cut(s) 200, 236, 245, 252, 299, 377, 404, 513
Lsp1109I GCAGC 1 cut(s) 148
LweI GCATC 2 cut(s) 109, 345
MalI GATC 5 cut(s) 5, 171, 219, 336, 492
MboI GATC 5 cut(s) 3, 169, 217, 334, 490
MboII GAAGA 5 cut(s) 9, 164, 418, 485, 488
MfeI CAATTG 1 cut(s) 165
MflI RGATCY 2 cut(s) 217, 490
MluCI AATT 5 cut(s) 30, 47, 165, 283, 293
Mly113I GGCGCC 1 cut(s) 398
MmeI TCCRAC 1 cut(s) 96
MnlI CCTC 6 cut(s) 109, 175, 360, 366, 388, 481
MseI TTAA 2 cut(s) 29, 290
MslI CAYNNNNRTG 1 cut(s) 194
MunI CAATTG 1 cut(s) 165
Mva1269I GAATGC 1 cut(s) 133
MwoI GCNNNNNNNGC 6 cut(s) 71, 128, 220, 256, 376, 397
NarI GGCGCC 1 cut(s) 398
NdeII GATC 5 cut(s) 3, 169, 217, 334, 490
NlaIII CATG 3 cut(s) 193, 199, 250
NlaIV GGNNCC 3 cut(s) 301, 399, 492
NmeAIII GCCGAG 2 cut(s) 80, 157
NspI RCATGY 1 cut(s) 199
NspV TTCGAA 1 cut(s) 41
PaeI GCATGC 1 cut(s) 199
PctI GAATGC 1 cut(s) 133
PfeI GAWTC 1 cut(s) 43
PkrI GCNGC 2 cut(s) 163, 402
PluTI GGCGCC 1 cut(s) 401
PspN4I GGNNCC 3 cut(s) 301, 399, 492
PspPI GGNCC 1 cut(s) 300
PstNI CAGNNNCTG 1 cut(s) 220
PsuI RGATCY 2 cut(s) 217, 490
RseI CAYNNNNRTG 1 cut(s) 194
SaqAI TTAA 2 cut(s) 29, 290
SatI GCNGC 2 cut(s) 162, 401
Sau3AI GATC 5 cut(s) 3, 169, 217, 334, 490
Sau96I GGNCC 1 cut(s) 300
SetI ASST 2 cut(s) 385, 505
SfaNI GCATC 2 cut(s) 109, 345
SfcI CTRYAG 1 cut(s) 177
SfoI GGCGCC 1 cut(s) 399
SfuI TTCGAA 1 cut(s) 41
SmiMI CAYNNNNRTG 1 cut(s) 194
SphI GCATGC 1 cut(s) 199
Sse9I AATT 5 cut(s) 30, 47, 165, 283, 293
SsiI CCGC 1 cut(s) 401
SspDI GGCGCC 1 cut(s) 397
TaaI ACNGT 2 cut(s) 85, 327
TaqI TCGA 1 cut(s) 41
TasI AATT 5 cut(s) 30, 47, 165, 283, 293
TauI GCSGC 1 cut(s) 403
TfiI GAWTC 1 cut(s) 43
Tru1I TTAA 2 cut(s) 29, 290
Tru9I TTAA 2 cut(s) 29, 290
TscAI CASTG 2 cut(s) 310, 330
TseI GCWGC 1 cut(s) 161
TspDTI ATGAA 3 cut(s) 133, 279, 458
TspRI CASTG 2 cut(s) 310, 330
XapI RAATTY 1 cut(s) 293
XceI RCATGY 1 cut(s) 199
XcmI CCANNNNNNNNNTGG 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.