MD14G1225900.v1.1

Probable lipid transfer

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0016222
Physical Location & Seq
Forward (+)
30662438 .. 30663413
976 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1225900.v1.1.491

Sequence Viewer

Length: 594 bp
ATGGCTTCCCCTAACAGTTGTCTGCTTCAAAATCATTTCGCCATTTCCTCTCTGACCCTACTCCTCTTTCTCATTTCCCTTCCGCCATCCGTCCTCTCACAAAACCCTACCATTGCGCAATGCACCACGCAGCTTCTTCCGCTGGCCGCTTGTGCACCATTTGTGCAAGGCAGTGCCATGTCACCTGCACAGTCATGCTGTGTCAACCTCAAGCTGCTATATAGCCAGCAGCCAGAGTGCCTTTGCCTTTTGCTCAATGGCACTACTTTGAGCACCTTCCTTATTAACACGACTCGCGCTCTCCAGCTTCCCGTTGTTTGCAGCCTTCAAGTCGACATCTCTACTTGTCCAGGGGTACCTGTGCCTCCTAGTTCACCTAGTTCTCAAGATCCCCCCCTTCGGAATAACACCAGCTCATCTGCTGCTAATTCTACAATTGCTGCCACTCCAATGCCTCAAACGGCACCAATCCCCACCACGATGGGGTTAGGGTTTGGGAGAACTACAAATGCTGGTACCAAATTGAAGATGGGAAGTTATCTTGTAGTGGCTTTCGCCGTGGCAGTTTTTCCAGTGACCGGAGTTCTATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2

Protein Analysis

198

Amino Acids

20.45

Weight (kDa)

8.43

Isoelectric Point (pI)

68.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 32 - 116 1.4e-15 Probable lipid transfer
Tryp_alpha_amyl PF00234 41 - 113 2.4e-09 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016222)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73890
fragaria_vesca FvH4_5g12310
malus_domestica MD06G1215100.v1.1 MD14G1225900.v1.1
prunus_persica Prupe.5G220400_v2.0.a1
pyrus_communis pycom06g19170
rosa_chinensis RchiOBHm_Chr7g0185251
rosa_laevigata RLG00000004927
rosa_roxburghii Rroxscaffold_3G00269110
rosa_rugosa Rorug06G0467300
rosa_samantha Rh7AG075500 Rh7BG071800 Rh7CG073000 Rh7DG072400
rosa_wichuraiana Rw7G006020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 193
Acc16I TGCGCA 1 cut(s) 117
Acc36I ACCTGC 1 cut(s) 193
Acc65I GGTACC 2 cut(s) 355, 515
AccB1I GGYRCC 3 cut(s) 355, 463, 515
AccI GTMKAC 1 cut(s) 333
AccII CGCG 1 cut(s) 297
AciI CCGC 3 cut(s) 83, 140, 147
AclWI GGATC 1 cut(s) 383
AcoI YGGCCR 1 cut(s) 144
AfaI GTAC 2 cut(s) 357, 517
AfiI CCNNNNNNNGG 3 cut(s) 399, 483, 578
AgsI TTSAA 3 cut(s) 29, 329, 526
AjnI CCWGG 1 cut(s) 349
AluBI AGCT 4 cut(s) 133, 214, 307, 414
AluI AGCT 4 cut(s) 133, 214, 307, 414
Alw21I GWGCWC 2 cut(s) 157, 275
Alw44I GTGCAC 1 cut(s) 153
AlwI GGATC 1 cut(s) 383
AoxI GGCC 1 cut(s) 144
ApaLI GTGCAC 1 cut(s) 153
ApeKI GCWGC 6 cut(s) 130, 214, 229, 321, 422, 440
Asp718I GGTACC 2 cut(s) 355, 515
AspLEI GCGC 2 cut(s) 118, 299
AsuHPI GGTGA 2 cut(s) 174, 366
BaeGI GKGCMC 1 cut(s) 157
BanI GGYRCC 3 cut(s) 355, 463, 515
Bbv12I GWGCWC 2 cut(s) 157, 275
BbvI GCAGC 6 cut(s) 142, 201, 241, 333, 409, 427
BccI CCATC 3 cut(s) 94, 475, 523
BceAI ACGGC 2 cut(s) 477, 542
BciT130I CCWGG 1 cut(s) 351
BfaI CTAG 2 cut(s) 369, 378
BfuAI ACCTGC 1 cut(s) 193
BisI GCNGC 7 cut(s) 131, 147, 215, 230, 322, 423, 441
BlsI GCNGC 7 cut(s) 132, 148, 216, 231, 323, 424, 442
Bme1390I CCNGG 1 cut(s) 351
BmiI GGNNCC 3 cut(s) 357, 465, 517
BmrFI CCNGG 1 cut(s) 351
BpmI CTGGAG 1 cut(s) 287
BpuEI CTTGAG 2 cut(s) 194, 369
BsaJI CCNNGG 2 cut(s) 350, 558
BsaWI WCCGGW 1 cut(s) 578
Bsc4I CCNNNNNNNGG 3 cut(s) 399, 483, 578
Bse1I ACTGG 1 cut(s) 572
Bse3DI GCAATG 2 cut(s) 111, 125
BseBI CCWGG 1 cut(s) 351
BseDI CCNNGG 2 cut(s) 350, 558
BseGI GGATG 1 cut(s) 86
BseLI CCNNNNNNNGG 3 cut(s) 399, 483, 578
BseMI GCAATG 2 cut(s) 111, 125
BseNI ACTGG 1 cut(s) 572
BseRI GAGGAG 1 cut(s) 53
BseSI GKGCMC 1 cut(s) 157
BseXI GCAGC 6 cut(s) 142, 201, 241, 333, 409, 427
BsgI GTGCAG 1 cut(s) 171
Bsh1236I CGCG 1 cut(s) 297
BshFI GGCC 1 cut(s) 146
BshNI GGYRCC 3 cut(s) 355, 463, 515
BsiHKAI GWGCWC 2 cut(s) 157, 275
BsiSI CCGG 1 cut(s) 579
BslI CCNNNNNNNGG 3 cut(s) 399, 483, 578
BsnI GGCC 1 cut(s) 146
Bsp1286I GDGCHC 2 cut(s) 157, 275
Bsp143I GATC 1 cut(s) 388
BspACI CCGC 3 cut(s) 83, 140, 147
BspANI GGCC 1 cut(s) 146
BspFNI CGCG 1 cut(s) 297
BspLI GGNNCC 3 cut(s) 357, 465, 517
BspMI ACCTGC 1 cut(s) 193
BspPI GGATC 1 cut(s) 383
BspT107I GGYRCC 3 cut(s) 355, 463, 515
BsrDI GCAATG 2 cut(s) 111, 125
BsrI ACTGG 1 cut(s) 572
BssECI CCNNGG 2 cut(s) 350, 558
BssMI GATC 1 cut(s) 388
Bst2UI CCWGG 1 cut(s) 351
Bst4CI ACNGT 2 cut(s) 17, 192
BstC8I GCNNGC 2 cut(s) 144, 227
BstDSI CCRYGG 1 cut(s) 558
BstF5I GGATG 1 cut(s) 86
BstFNI CGCG 1 cut(s) 297
BstHHI GCGC 2 cut(s) 118, 299
BstKTI GATC 1 cut(s) 391
BstMBI GATC 1 cut(s) 388
BstMWI GCNNNNNNNGC 2 cut(s) 139, 152
BstNI CCWGG 1 cut(s) 351
BstSCI CCNGG 1 cut(s) 349
BstSLI GKGCMC 1 cut(s) 157
BstUI CGCG 1 cut(s) 297
BstV1I GCAGC 6 cut(s) 142, 201, 241, 333, 409, 427
BstX2I RGATCY 1 cut(s) 388
BstXI CCANNNNNNTGG 1 cut(s) 481
BstYI RGATCY 1 cut(s) 388
BsuRI GGCC 1 cut(s) 146
BtgI CCRYGG 1 cut(s) 558
BtsCI GGATG 1 cut(s) 86
BtsI GCAGTG 1 cut(s) 178
BtsIMutI CAGTG 2 cut(s) 178, 579
BveI ACCTGC 1 cut(s) 193
Cac8I GCNNGC 2 cut(s) 144, 227
CfoI GCGC 2 cut(s) 118, 299
Csp6I GTAC 2 cut(s) 356, 516
CviAII CATG 2 cut(s) 178, 195
CviQI GTAC 2 cut(s) 356, 516
DpnI GATC 1 cut(s) 390
DpnII GATC 1 cut(s) 388
EaeI YGGCCR 1 cut(s) 144
EciI GGCGGA 1 cut(s) 72
EcoRII CCWGG 1 cut(s) 349
FaeI CATG 2 cut(s) 181, 198
FaiI YATR 4 cut(s) 179, 196, 220, 222
FatI CATG 2 cut(s) 177, 194
FblI GTMKAC 1 cut(s) 333
Fnu4HI GCNGC 7 cut(s) 131, 147, 215, 230, 322, 423, 441
FokI GGATG 1 cut(s) 73
Fsp4HI GCNGC 7 cut(s) 131, 147, 215, 230, 322, 423, 441
FspBI CTAG 2 cut(s) 369, 378
FspI TGCGCA 1 cut(s) 117
GlaI GCGC 2 cut(s) 117, 298
GluI GCNGC 7 cut(s) 131, 147, 215, 230, 322, 423, 441
GsuI CTGGAG 1 cut(s) 287
HaeIII GGCC 1 cut(s) 146
HapII CCGG 1 cut(s) 579
HhaI GCGC 2 cut(s) 118, 299
Hin1II CATG 2 cut(s) 181, 198
Hin6I GCGC 2 cut(s) 116, 297
HinP1I GCGC 2 cut(s) 116, 297
HincII GTYRAC 2 cut(s) 205, 334
HindII GTYRAC 2 cut(s) 205, 334
HinfI GANTC 1 cut(s) 292
HpaII CCGG 1 cut(s) 579
HphI GGTGA 2 cut(s) 174, 366
Hpy166II GTNNAC 4 cut(s) 155, 205, 334, 374
Hpy188I TCNGA 2 cut(s) 54, 402
Hpy188III TCNNGA 1 cut(s) 386
Hpy8I GTNNAC 4 cut(s) 155, 205, 334, 374
HpyAV CCTTC 4 cut(s) 89, 286, 335, 407
HpyCH4III ACNGT 2 cut(s) 17, 192
HpyCH4V TGCA 5 cut(s) 123, 155, 166, 188, 321
HpyF10VI GCNNNNNNNGC 2 cut(s) 139, 152
Hsp92II CATG 2 cut(s) 181, 198
HspAI GCGC 2 cut(s) 116, 297
KpnI GGTACC 2 cut(s) 359, 519
Kzo9I GATC 1 cut(s) 388
Lsp1109I GCAGC 6 cut(s) 142, 201, 241, 333, 409, 427
MaeI CTAG 2 cut(s) 369, 378
MaeIII GTNAC 2 cut(s) 180, 574
MalI GATC 1 cut(s) 390
MboI GATC 1 cut(s) 388
MboII GAAGA 2 cut(s) 128, 538
MfeI CAATTG 1 cut(s) 435
MflI RGATCY 1 cut(s) 388
MhlI GDGCHC 2 cut(s) 157, 275
MluCI AATT 3 cut(s) 427, 435, 521
MlyI GAGTC 1 cut(s) 286
MnlI CCTC 6 cut(s) 58, 74, 104, 218, 375, 465
MseI TTAA 2 cut(s) 285, 592
MslI CAYNNNNRTG 3 cut(s) 193, 449, 479
MspA1I CMGCKG 1 cut(s) 142
MspI CCGG 1 cut(s) 579
MspR9I CCNGG 1 cut(s) 351
MunI CAATTG 1 cut(s) 435
MvaI CCWGG 1 cut(s) 351
MvnI CGCG 1 cut(s) 297
MwoI GCNNNNNNNGC 2 cut(s) 139, 152
NdeII GATC 1 cut(s) 388
NlaIII CATG 2 cut(s) 181, 198
NlaIV GGNNCC 3 cut(s) 357, 465, 517
NmuCI GTSAC 2 cut(s) 180, 574
NsbI TGCGCA 1 cut(s) 117
PaqCI CACCTGC 1 cut(s) 193
PkrI GCNGC 7 cut(s) 132, 148, 216, 231, 323, 424, 442
PleI GAGTC 1 cut(s) 286
PpsI GAGTC 1 cut(s) 286
Psp6I CCWGG 1 cut(s) 349
PspGI CCWGG 1 cut(s) 349
PspN4I GGNNCC 3 cut(s) 357, 465, 517
PsuI RGATCY 1 cut(s) 388
RsaI GTAC 2 cut(s) 357, 517
RsaNI GTAC 2 cut(s) 356, 516
RseI CAYNNNNRTG 3 cut(s) 193, 449, 479
SalI GTCGAC 1 cut(s) 332
SaqAI TTAA 2 cut(s) 285, 592
SatI GCNGC 7 cut(s) 131, 147, 215, 230, 322, 423, 441
Sau3AI GATC 1 cut(s) 388
SchI GAGTC 1 cut(s) 286
ScrFI CCNGG 1 cut(s) 351
SduI GDGCHC 2 cut(s) 157, 275
SetI ASST 9 cut(s) 135, 187, 210, 216, 278, 309, 361, 379, 416
SmiMI CAYNNNNRTG 3 cut(s) 193, 449, 479
SmlI CTYRAG 2 cut(s) 209, 384
SmoI CTYRAG 2 cut(s) 209, 384
Sse9I AATT 3 cut(s) 427, 435, 521
SsiI CCGC 3 cut(s) 83, 140, 147
SspMI CTAG 2 cut(s) 369, 378
StyD4I CCNGG 1 cut(s) 349
TaaI ACNGT 2 cut(s) 17, 192
TaqI TCGA 1 cut(s) 333
TasI AATT 3 cut(s) 427, 435, 521
TauI GCSGC 1 cut(s) 149
Tru1I TTAA 2 cut(s) 285, 592
Tru9I TTAA 2 cut(s) 285, 592
TscAI CASTG 2 cut(s) 178, 579
TseFI GTSAC 2 cut(s) 180, 574
TseI GCWGC 6 cut(s) 130, 214, 229, 321, 422, 440
Tsp45I GTSAC 2 cut(s) 180, 574
TspGWI ACGGA 1 cut(s) 79
TspRI CASTG 2 cut(s) 178, 579
VneI GTGCAC 1 cut(s) 153
XcmI CCANNNNNNNNNTGG 1 cut(s) 526
XmiI GTMKAC 1 cut(s) 333
XspI CTAG 2 cut(s) 369, 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.