Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0012830

Physical Location & Seq

Genomic Coordinates Forward (+)

547249 .. 553650

Gene Structure 6402 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1010200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2379 bp

ATGAGATCGGACGTAATCGGAGACGAAGAGAAATGGCTGGCGGAAGGCATCGCCGGCATTCAACACCATGCCTTCTACATGCATCGCGCTCTGGACGCCAACAATCTCAGAGACGCCCTCAAATTCTCGGCCCTGATGCTGTCGGAGCTCAGAACCTCTCGGCTTTCGCCTCACAAATACTACGATCTCTATATGCGAGCTTTCGATGAGCTGAGGAGGCTGGAGATGTTCTTCAAGGACGAGAGCAGACACGGCGTTTCGATCATCGATCTCTACGAACTTGTTCAGCATGCCGGCAACATTTTACCAAGATTGTATCTGCTATGTACGGTAGGATCCGTGTACATTAAATCTAAGGAAGCGCCAGCCAAGGACGTACTTAAAGATCTTGTCGAAATGTGTCGCGGCGTTCAGCATCCAATTCGAGGGCTCTTTTTAAGAAGCTATCTTTCTCAAGTCAGCAGAGACAAGTTGCCTGATATTGGTTCTGAGTATGAAGGGGATGCTGACACTGTCATGGATGCTGTAGATTTTGTGCTACAGAATTTTACAGAAATGAATAAGCTTTGGGTTCGAATGCAGTATCAGGGACCTGGTCATGTGAGAGAGAAGCGCGAAAAGGAAAGAAGCGAACTTCGTGATCTTGTTGGGAAGAATCTCCATGTTCTCAGTCAGATAGAGGGTGTGGAACTTGAAATGTACAAAGCCACTGTTCTTCCTAGAGTATTAGAACAGGTTATCAACTGTAAAGATGAGCTGGCCCAATATTATTTGATGGATTGCATAATCCAGGTCTTTCCAGATGAGTACCACTTGCAGACGCTGGAGACATTGTTGGCTGCCTTCCCCCAGCTTCAGCCAACAGTTGATATCAAGACTGTGTTATCTCAATTAATGGAGAGGTTATCAAACTATGCTGCGTCAAGTACAGATGTATTACCTGAATTTCTTCAAGTGGAAGCCTTCGCTAAATTAAGCAGTGCCATTGGGTGGGTGATAGAAGCACAAACGGACATGCCTATTGTTGGAGCCATATCTTTGTATGTCTCTCTTCTTACATTTACCCTCCGTGTTCATCCTGATCGCCTTGATTATGTGGATCAAGTACTGGGAGCATGTGTTAAGAAGCTATCCGGAGAAACCAAACTTGAAGACCATAGAGCAACAAAACAGGTTGTTGCACTGTTAAGTGCTCCCTTGGAGAAATACGACGACATTGTCACAGCCCTCACACTTTCTAATTATCCTCGAGTTATGGATTATCTTGATAATGGAACAAATAAAGTCATGGCAATGGTCATTATCCAAAGCATTATGAAGAACAACTCTTGTATCTCCACAGCTGATAAGGTGGAGGTGTTGTTTGAATTAATTAAAGGGCTCATCAAGGACTTGGATTCTACTTCTGCAGATGAGCTTGATGAAGAGGATTTTGCGGATGAACAAAATTCTGTTGCTCGCCTTATACACATGCTTTATAATGATGATCCAGAAGAAATGTTTAAGATCTTATGTACGGTCAAGAAGCATATAATGGGTGGAGGACCAAAGCGCCTACCTTTTACTGTACCTCCTCTTATATTATCTGCACTCAAGTTGGTTAGGCGGCTGCAAGGTCAGGATGGAGAGGTTGTGGGAGAAGAAATGCCTGCCACACCGAAGAAAATTTTTCAGACTTTAAATCAGATCATTGAATCTCTTTCCTCAGTTCCGTCTCCCGAACTGGCATTAAGATTGTACTTGGAATGTGCTGAGGCTTCTAATGACTGTGATCTGGAACCTGTTGCTTATGAATTCTTCACTCAGGCATTTATATTATACGAAGAAGAAGTTGCGGATTCCAAAGCCCAGGTGACTGCAATACATCTCATTATTGGGACTCTTCAGAGGATGAATGTATTTGGCGTTGAGAATCGGGATACTTTAACACACAAGGCTACTGGATATTCAGCAAAGCTTTTGAAGAAACCTGATCAGTGCCGAGCGGTGTATGCATGTTCACATCTTTTTTGGGTAGATGATCAGGATGGTGTTAAAGATGGAGAAAGGGTCTTGCTTTGTTTGAAACGCGCATTGAGAATTGCAAATGCTGCTCAACAGATGGCCAGCGCAACGCGGGGTAGCAGCGGGCCAGTCCCAGTCACACTCTTTGTTGAGATACTGAACAAGTACCTCTACTATTTTGAGAAAGGAAACCCCCAGATTACAAGTGCTGCAATCCAGGGATTAGTGGATTTAATTAAAAATGAGATGCAAGGTGACTCTGCAAACGCGAATCCAGCCCCAGATGCATTTTTCGCCAGCACATTGCGGTACATACAGTTCCAAAAACAGAAAGGTGGGGTGATGGGCGAGAAATATGCCTCTATCAAGGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0006810 GO:0006886 GO:0008104 GO:0008150 GO:0008565 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016192 GO:0016197 GO:0016482 GO:0030904 GO:0031410 GO:0031982 GO:0032991 GO:0033036 GO:0034613 GO:0042147 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071705 GO:0097708 GO:0098796

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

Vps35

Protein Analysis

793

Amino Acids

89.31

Weight (kDa)

5.3

Isoelectric Point (pI)

41.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Vps35	PF03635	12 - 749	5e-288	Vacuolar protein sorting-associated protein 35

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0012830)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G75850 AT1G75850
fragaria_vesca	FvH4_2g38180 FvH4_2g38180
malus_domestica	MD15G1010200.v1.1
prunus_persica	Prupe.1G362700_v2.0.a1
pyrus_communis	pycom15g00840
rosa_chinensis	RchiOBHm_Chr6g0303301
rosa_laevigata	RLG00000011094
rosa_multiflora	Rmu_sc0006346.1_g000001 Rmu_sc0019430.1_g000001
rosa_roxburghii	Rroxscaffold_7G00164890
rosa_rugosa	Rorug06G0320200
rosa_samantha	Rh6AG434600 Rh6BG468500 Rh6CG446600 Rh6DG433300
rosa_wichuraiana	Rw6G037630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1479
AasI	GACNNNNNNGTC	1 cut(s)	1217
AccB7I	CCANNNNNTGG	1 cut(s)	990
AccBSI	CCGCTC	1 cut(s)	1985
AccII	CGCG	6 cut(s)	87, 405, 615, 2070, 2116, 2273
AccIII	TCCGGA	1 cut(s)	1133
AciI	CCGC	9 cut(s)	41, 405, 1436, 1606, 1835, 1985, 2116, 2127, 2311
AclWI	GGATC	4 cut(s)	330, 343, 1107, 1481
AcoI	YGGCCR	1 cut(s)	2103
AcsI	RAATTY	6 cut(s)	122, 544, 944, 1447, 1665, 1793
AcuI	CTGAAG	2 cut(s)	839, 1868
AcyI	GRCGYC	2 cut(s)	96, 114
AfiI	CCNNNNNNNGG	4 cut(s)	425, 482, 990, 1025
AgsI	TTSAA	9 cut(s)	62, 235, 695, 953, 1151, 1367, 1694, 1963, 2065
AjnI	CCWGG	4 cut(s)	592, 789, 1848, 2220
Alw21I	GWGCWC	2 cut(s)	150, 1195
Alw26I	GTCTC	6 cut(s)	15, 105, 459, 821, 1051, 1719
AlwI	GGATC	4 cut(s)	330, 343, 1107, 1481
AlwNI	CAGNNNCTG	1 cut(s)	823
Ama87I	CYCGRG	1 cut(s)	1248
Aor13HI	TCCGGA	1 cut(s)	1133
AoxI	GGCC	4 cut(s)	129, 759, 2103, 2129
ApeKI	GCWGC	6 cut(s)	839, 917, 1609, 2090, 2124, 2213
ApoI	RAATTY	6 cut(s)	122, 544, 944, 1447, 1665, 1793
AseI	ATTAAT	2 cut(s)	893, 1370
Asp700I	GAANNNNTTC	2 cut(s)	282, 948
AspLEI	GCGC	6 cut(s)	89, 364, 615, 1554, 2072, 2111
AspS9I	GGNCC	5 cut(s)	130, 590, 760, 1544, 2129
AsuHPI	GGTGA	4 cut(s)	1006, 1864, 2270, 2356
AsuII	TTCGAA	1 cut(s)	574
AvaI	CYCGRG	1 cut(s)	1248
AvaII	GGWCC	2 cut(s)	590, 1544
BaeI	ACNNNNGTAYC	2 cut(s)	1315, 1348
BalI	TGGCCA	1 cut(s)	2105
BamHI	GGATCC	1 cut(s)	335
BanII	GRGCYC	3 cut(s)	150, 432, 1383
BbsI	GAAGAC	1 cut(s)	1158
Bbv12I	GWGCWC	2 cut(s)	150, 1195
BbvCI	CCTCAGC	2 cut(s)	212, 1752
BbvI	GCAGC	6 cut(s)	826, 904, 1596, 2077, 2136, 2200
BccI	CCATC	6 cut(s)	769, 1616, 2021, 2033, 2095, 2341
BceAI	ACGGC	1 cut(s)	268
BcgI	CGANNNNNNTGC	4 cut(s)	404, 438, 2342, 2376
BciT130I	CCWGG	4 cut(s)	594, 791, 1850, 2222
BciVI	GTATCC	1 cut(s)	1912
BclI	TGATCA	2 cut(s)	1972, 2020
BcoDI	GTCTC	6 cut(s)	15, 105, 459, 821, 1051, 1719
BfaI	CTAG	1 cut(s)	720
BfmI	CTRYAG	3 cut(s)	525, 539, 1407
BfoI	RGCGCY	2 cut(s)	365, 1555
BfuI	GTATCC	1 cut(s)	1912
BglII	AGATCT	2 cut(s)	385, 1506
BisI	GCNGC	8 cut(s)	406, 840, 918, 1607, 1610, 2091, 2125, 2214
BlsI	GCNGC	8 cut(s)	407, 841, 919, 1608, 1611, 2092, 2126, 2215
BmcAI	AGTACT	1 cut(s)	1107
Bme1390I	CCNGG	4 cut(s)	594, 791, 1850, 2222
Bme18I	GGWCC	2 cut(s)	590, 1544
BmeT110I	CYCGRG	1 cut(s)	1248
BmgT120I	GGNCC	5 cut(s)	130, 590, 760, 1544, 2129
BmiI	GGNNCC	4 cut(s)	337, 591, 1030, 1779
BmrFI	CCNGG	4 cut(s)	594, 791, 1850, 2222
BmrI	ACTGGG	2 cut(s)	1118, 2132
BmsI	GCATC	8 cut(s)	57, 91, 126, 424, 493, 511, 2241, 2278
BmuI	ACTGGG	2 cut(s)	1118, 2132
BpiI	GAAGAC	1 cut(s)	1158
BplI	GAGNNNNNCTC	2 cut(s)	102, 134
BpmI	CTGGAG	2 cut(s)	242, 845
Bpu10I	CCTNAGC	2 cut(s)	212, 1752
Bpu14I	TTCGAA	1 cut(s)	574
BpuEI	CTTGAG	2 cut(s)	438, 1577
Bsa29I	ATCGAT	1 cut(s)	267
BsaHI	GRCGYC	2 cut(s)	96, 114
BsaJI	CCNNGG	4 cut(s)	369, 1197, 1848, 2221
BsaWI	WCCGGW	1 cut(s)	1133
Bsc4I	CCNNNNNNNGG	4 cut(s)	425, 482, 990, 1025
Bse118I	RCCGGY	2 cut(s)	53, 293
Bse1I	ACTGG	5 cut(s)	1113, 1728, 1945, 2132, 2138
Bse3DI	GCAATG	2 cut(s)	1299, 2306
BseAI	TCCGGA	1 cut(s)	1133
BseBI	CCWGG	4 cut(s)	594, 791, 1850, 2222
BseCI	ATCGAT	1 cut(s)	267
BseDI	CCNNGG	4 cut(s)	369, 1197, 1848, 2221
BseGI	GGATG	8 cut(s)	415, 508, 526, 1075, 1444, 1627, 1896, 2032
BseLI	CCNNNNNNNGG	4 cut(s)	425, 482, 990, 1025
BseMI	GCAATG	2 cut(s)	1299, 2306
BseMII	CTCAG	8 cut(s)	121, 163, 203, 480, 682, 1719, 1743, 1817
BseNI	ACTGG	5 cut(s)	1113, 1728, 1945, 2132, 2138
BseRI	GAGGAG	2 cut(s)	229, 1563
BseXI	GCAGC	6 cut(s)	826, 904, 1596, 2077, 2136, 2200
BseYI	CCCAGC	1 cut(s)	849
BsgI	GTGCAG	1 cut(s)	1572
Bsh1236I	CGCG	6 cut(s)	87, 405, 615, 2070, 2116, 2273
BshFI	GGCC	4 cut(s)	131, 761, 2105, 2131
BshVI	ATCGAT	1 cut(s)	267
BsiHKAI	GWGCWC	2 cut(s)	150, 1195
BsiHKCI	CYCGRG	1 cut(s)	1248
BsiSI	CCGG	3 cut(s)	54, 294, 1134
BslFI	GGGAC	3 cut(s)	603, 1891, 2120
BslI	CCNNNNNNNGG	4 cut(s)	425, 482, 990, 1025
BsmAI	GTCTC	6 cut(s)	15, 105, 459, 821, 1051, 1719
BsmBI	CGTCTC	3 cut(s)	15, 105, 1719
BsmFI	GGGAC	3 cut(s)	603, 1891, 2120
BsmI	GAATGC	2 cut(s)	57, 582
BsnI	GGCC	4 cut(s)	131, 761, 2105, 2131
BsoBI	CYCGRG	1 cut(s)	1248
Bsp119I	TTCGAA	1 cut(s)	574
Bsp1286I	GDGCHC	4 cut(s)	150, 432, 1195, 1383
Bsp13I	TCCGGA	1 cut(s)	1133
Bsp1407I	TGTACA	2 cut(s)	342, 699
BspACI	CCGC	9 cut(s)	41, 405, 1436, 1606, 1835, 1985, 2116, 2127, 2311
BspANI	GGCC	4 cut(s)	131, 761, 2105, 2131
BspCNI	CTCAG	8 cut(s)	120, 162, 204, 481, 681, 1718, 1744, 1816
BspDI	ATCGAT	1 cut(s)	267
BspEI	TCCGGA	1 cut(s)	1133
BspFNI	CGCG	6 cut(s)	87, 405, 615, 2070, 2116, 2273
BspLI	GGNNCC	4 cut(s)	337, 591, 1030, 1779
BspMAI	CTGCAG	1 cut(s)	1411
BspPI	GGATC	4 cut(s)	330, 343, 1107, 1481
BspT104I	TTCGAA	1 cut(s)	574
BsrBI	CCGCTC	1 cut(s)	1985
BsrDI	GCAATG	2 cut(s)	1299, 2306
BsrFI	RCCGGY	2 cut(s)	53, 293
BsrGI	TGTACA	2 cut(s)	342, 699
BsrI	ACTGG	5 cut(s)	1113, 1728, 1945, 2132, 2138
BssAI	RCCGGY	2 cut(s)	53, 293
BssECI	CCNNGG	4 cut(s)	369, 1197, 1848, 2221
BssNI	GRCGYC	2 cut(s)	96, 114
BssT1I	CCWWGG	2 cut(s)	369, 1197
Bst2UI	CCWGG	4 cut(s)	594, 791, 1850, 2222
Bst6I	CTCTTC	4 cut(s)	21, 1056, 1419, 1887
BstACI	GRCGYC	2 cut(s)	96, 114
BstAPI	GCANNNNNTGC	1 cut(s)	2090
BstAUI	TGTACA	2 cut(s)	342, 699
BstBI	TTCGAA	1 cut(s)	574
BstDEI	CTNAG	9 cut(s)	107, 149, 212, 354, 489, 668, 1705, 1752, 1803
BstF5I	GGATG	8 cut(s)	415, 508, 526, 1075, 1444, 1627, 1896, 2032
BstFNI	CGCG	6 cut(s)	87, 405, 615, 2070, 2116, 2273
BstH2I	RGCGCY	2 cut(s)	365, 1555
BstHHI	GCGC	6 cut(s)	89, 364, 615, 1554, 2072, 2111
BstMAI	GTCTC	6 cut(s)	15, 105, 459, 821, 1051, 1719
BstNI	CCWGG	4 cut(s)	594, 791, 1850, 2222
BstNSI	RCATGY	6 cut(s)	82, 293, 1018, 1119, 1474, 1998
BstSCI	CCNGG	4 cut(s)	592, 789, 1848, 2220
BstSFI	CTRYAG	3 cut(s)	525, 539, 1407
BstUI	CGCG	6 cut(s)	87, 405, 615, 2070, 2116, 2273
BstV1I	GCAGC	6 cut(s)	826, 904, 1596, 2077, 2136, 2200
BstV2I	GAAGAC	1 cut(s)	1158
BstX2I	RGATCY	3 cut(s)	335, 385, 1506
BstYI	RGATCY	3 cut(s)	335, 385, 1506
Bsu15I	ATCGAT	1 cut(s)	267
BsuI	GTATCC	1 cut(s)	1912
BsuRI	GGCC	4 cut(s)	131, 761, 2105, 2131
BsuTUI	ATCGAT	1 cut(s)	267
BtgZI	GCGATG	2 cut(s)	34, 68
BtsCI	GGATG	8 cut(s)	415, 508, 526, 1075, 1444, 1627, 1896, 2032
BtsI	GCAGTG	1 cut(s)	985
BtsIMutI	CAGTG	5 cut(s)	510, 708, 985, 1181, 1982
CaiI	CAGNNNCTG	1 cut(s)	823
CfoI	GCGC	6 cut(s)	89, 364, 615, 1554, 2072, 2111
Cfr10I	RCCGGY	2 cut(s)	53, 293
Cfr13I	GGNCC	5 cut(s)	130, 590, 760, 1544, 2129
ClaI	ATCGAT	1 cut(s)	267
CseI	GACGC	4 cut(s)	104, 122, 829, 909
CsiI	ACCWGGT	1 cut(s)	592
DdeI	CTNAG	9 cut(s)	107, 149, 212, 354, 489, 668, 1705, 1752, 1803
DraI	TTTAAA	1 cut(s)	1680
DrdI	GACNNNNNNGTC	1 cut(s)	1217
DseDI	GACNNNNNNGTC	1 cut(s)	1217
EaeI	YGGCCR	1 cut(s)	2103
Eam1104I	CTCTTC	4 cut(s)	21, 1056, 1419, 1887
EarI	CTCTTC	4 cut(s)	21, 1056, 1419, 1887
EciI	GGCGGA	1 cut(s)	56
Ecl136II	GAGCTC	1 cut(s)	148
Eco130I	CCWWGG	2 cut(s)	369, 1197
Eco24I	GRGCYC	3 cut(s)	150, 432, 1383
Eco32I	GATATC	1 cut(s)	871
Eco47I	GGWCC	2 cut(s)	590, 1544
Eco53kI	GAGCTC	1 cut(s)	148
Eco57I	CTGAAG	2 cut(s)	839, 1868
Eco88I	CYCGRG	1 cut(s)	1248
EcoICRI	GAGCTC	1 cut(s)	148
EcoO109I	RGGNCCY	1 cut(s)	590
EcoRI	GAATTC	1 cut(s)	1793
EcoRII	CCWGG	4 cut(s)	592, 789, 1848, 2220
EcoRV	GATATC	1 cut(s)	871
EcoT14I	CCWWGG	2 cut(s)	369, 1197
EcoT22I	ATGCAT	3 cut(s)	84, 1996, 2293
EcoT38I	GRGCYC	3 cut(s)	150, 432, 1383
ErhI	CCWWGG	2 cut(s)	369, 1197
Esp3I	CGTCTC	3 cut(s)	15, 105, 1719
FaqI	GGGAC	3 cut(s)	603, 1891, 2120
FauI	CCCGC	2 cut(s)	2109, 2120
FbaI	TGATCA	2 cut(s)	1972, 2020
Fnu4HI	GCNGC	8 cut(s)	406, 840, 918, 1607, 1610, 2091, 2125, 2214
FokI	GGATG	8 cut(s)	402, 515, 533, 1062, 1451, 1634, 1903, 2039
FriOI	GRGCYC	3 cut(s)	150, 432, 1383
Fsp4HI	GCNGC	8 cut(s)	406, 840, 918, 1607, 1610, 2091, 2125, 2214
FspBI	CTAG	1 cut(s)	720
GlaI	GCGC	6 cut(s)	88, 363, 614, 1553, 2071, 2110
GluI	GCNGC	8 cut(s)	406, 840, 918, 1607, 1610, 2091, 2125, 2214
GsaI	CCCAGC	1 cut(s)	853
GsuI	CTGGAG	2 cut(s)	242, 845
HaeII	RGCGCY	2 cut(s)	365, 1555
HaeIII	GGCC	4 cut(s)	131, 761, 2105, 2131
HapII	CCGG	3 cut(s)	54, 294, 1134
HgaI	GACGC	4 cut(s)	104, 122, 829, 909
HhaI	GCGC	6 cut(s)	89, 364, 615, 1554, 2072, 2111
Hin1I	GRCGYC	2 cut(s)	96, 114
Hin6I	GCGC	6 cut(s)	87, 362, 613, 1552, 2070, 2109
HinP1I	GCGC	6 cut(s)	87, 362, 613, 1552, 2070, 2109
HindIII	AAGCTT	2 cut(s)	563, 1955
HinfI	GANTC	8 cut(s)	655, 1397, 1694, 1838, 1879, 1912, 2261, 2275
HpaII	CCGG	3 cut(s)	54, 294, 1134
HphI	GGTGA	4 cut(s)	1006, 1864, 2270, 2356
Hpy166II	GTNNAC	2 cut(s)	343, 2000
Hpy8I	GTNNAC	2 cut(s)	343, 2000
Hpy99I	CGWCG	1 cut(s)	1214
HpyAV	CCTTC	5 cut(s)	38, 82, 491, 853, 973
HpyCH4IV	ACGT	2 cut(s)	12, 375
HpyF3I	CTNAG	9 cut(s)	107, 149, 212, 354, 489, 668, 1705, 1752, 1803
HpySE526I	ACGT	2 cut(s)	12, 375
Hsp92I	GRCGYC	2 cut(s)	96, 114
HspAI	GCGC	6 cut(s)	87, 362, 613, 1552, 2070, 2109
Kpn2I	TCCGGA	1 cut(s)	1133
KroI	GCCGGC	2 cut(s)	53, 293
KroNI	GCCGGC	2 cut(s)	55, 295
Ksp22I	TGATCA	2 cut(s)	1972, 2020
LmnI	GCTCC	4 cut(s)	145, 1028, 1112, 1198
Lsp1109I	GCAGC	6 cut(s)	826, 904, 1596, 2077, 2136, 2200
LweI	GCATC	8 cut(s)	57, 91, 126, 424, 493, 511, 2241, 2278
MabI	ACCWGGT	1 cut(s)	592
MaeI	CTAG	1 cut(s)	720
MaeII	ACGT	2 cut(s)	12, 375
MaeIII	GTNAC	4 cut(s)	1219, 1852, 2140, 2258
MbiI	CCGCTC	1 cut(s)	1985
MflI	RGATCY	3 cut(s)	335, 385, 1506
MhlI	GDGCHC	4 cut(s)	150, 432, 1195, 1383
MlsI	TGGCCA	1 cut(s)	2105
MluNI	TGGCCA	1 cut(s)	2105
MlyI	GAGTC	2 cut(s)	1873, 2255
MmeI	TCCRAC	2 cut(s)	123, 1006
Mox20I	TGGCCA	1 cut(s)	2105
Mph1103I	ATGCAT	3 cut(s)	84, 1996, 2293
MroI	TCCGGA	1 cut(s)	1133
MroNI	GCCGGC	2 cut(s)	53, 293
MroXI	GAANNNNTTC	2 cut(s)	282, 948
MscI	TGGCCA	1 cut(s)	2105
MslI	CAYNNNNRTG	2 cut(s)	515, 1292
Msp20I	TGGCCA	1 cut(s)	2105
MspA1I	CMGCKG	2 cut(s)	1343, 2127
MspI	CCGG	3 cut(s)	54, 294, 1134
MspR9I	CCNGG	4 cut(s)	594, 791, 1850, 2222
Mva1269I	GAATGC	2 cut(s)	57, 582
MvaI	CCWGG	4 cut(s)	594, 791, 1850, 2222
MvnI	CGCG	6 cut(s)	87, 405, 615, 2070, 2116, 2273
NaeI	GCCGGC	2 cut(s)	55, 295
NgoMIV	GCCGGC	2 cut(s)	53, 293
NlaIV	GGNNCC	4 cut(s)	337, 591, 1030, 1779
NmeAIII	GCCGAG	3 cut(s)	107, 139, 2006
NmuCI	GTSAC	4 cut(s)	1219, 1852, 2140, 2258
NsiI	ATGCAT	3 cut(s)	84, 1996, 2293
NspI	RCATGY	6 cut(s)	82, 293, 1018, 1119, 1474, 1998
NspV	TTCGAA	1 cut(s)	574
PacI	TTAATTAA	2 cut(s)	1374, 2241
PaeI	GCATGC	1 cut(s)	293
PaeR7I	CTCGAG	1 cut(s)	1248
PcsI	WCGNNNNNNNCGW	1 cut(s)	273
PctI	GAATGC	2 cut(s)	57, 582
PdiI	GCCGGC	2 cut(s)	55, 295
PdmI	GAANNNNTTC	2 cut(s)	282, 948
PfeI	GAWTC	6 cut(s)	655, 1397, 1694, 1838, 1912, 2275
PflFI	GACNNNGTC	3 cut(s)	512, 594, 1217
PflMI	CCANNNNNTGG	1 cut(s)	990
PkrI	GCNGC	8 cut(s)	407, 841, 919, 1608, 1611, 2092, 2126, 2215
PleI	GAGTC	2 cut(s)	1873, 2255
PpsI	GAGTC	2 cut(s)	1873, 2255
PpuMI	RGGWCCY	1 cut(s)	590
PshBI	ATTAAT	2 cut(s)	893, 1370
PsiI	TTATAA	1 cut(s)	1479
Psp124BI	GAGCTC	1 cut(s)	150
Psp5II	RGGWCCY	1 cut(s)	590
Psp6I	CCWGG	4 cut(s)	592, 789, 1848, 2220
PspFI	CCCAGC	1 cut(s)	849
PspGI	CCWGG	4 cut(s)	592, 789, 1848, 2220
PspN4I	GGNNCC	4 cut(s)	337, 591, 1030, 1779
PspPI	GGNCC	5 cut(s)	130, 590, 760, 1544, 2129
PspPPI	RGGWCCY	1 cut(s)	590
PspXI	VCTCGAGB	1 cut(s)	1248
PsrI	GAACNNNNNNTAC	2 cut(s)	2306, 2338
PstI	CTGCAG	1 cut(s)	1411
PstNI	CAGNNNCTG	1 cut(s)	823
PsuI	RGATCY	3 cut(s)	335, 385, 1506
PsyI	GACNNNGTC	3 cut(s)	512, 594, 1217
PvuII	CAGCTG	1 cut(s)	1343
RseI	CAYNNNNRTG	2 cut(s)	515, 1292
SacI	GAGCTC	1 cut(s)	150
SatI	GCNGC	8 cut(s)	406, 840, 918, 1607, 1610, 2091, 2125, 2214
Sau96I	GGNCC	5 cut(s)	130, 590, 760, 1544, 2129
ScaI	AGTACT	1 cut(s)	1107
SchI	GAGTC	2 cut(s)	1873, 2255
ScrFI	CCNGG	4 cut(s)	594, 791, 1850, 2222
SduI	GDGCHC	4 cut(s)	150, 432, 1195, 1383
SexAI	ACCWGGT	1 cut(s)	592
SfaNI	GCATC	8 cut(s)	57, 91, 126, 424, 493, 511, 2241, 2278
SfcI	CTRYAG	3 cut(s)	525, 539, 1407
Sfr274I	CTCGAG	1 cut(s)	1248
SfuI	TTCGAA	1 cut(s)	574
SinI	GGWCC	2 cut(s)	590, 1544
SlaI	CTCGAG	1 cut(s)	1248
SmiMI	CAYNNNNRTG	2 cut(s)	515, 1292
SmlI	CTYRAG	3 cut(s)	453, 1248, 1592
SmoI	CTYRAG	3 cut(s)	453, 1248, 1592
SphI	GCATGC	1 cut(s)	293
SsiI	CCGC	9 cut(s)	41, 405, 1436, 1606, 1835, 1985, 2116, 2127, 2311
SspI	AATATT	1 cut(s)	767
SspMI	CTAG	1 cut(s)	720
SstI	GAGCTC	1 cut(s)	150
StyD4I	CCNGG	4 cut(s)	592, 789, 1848, 2220
StyI	CCWWGG	2 cut(s)	369, 1197
TaiI	ACGT	2 cut(s)	15, 378
TaqI	TCGA	7 cut(s)	204, 260, 267, 393, 424, 574, 1249
TatI	WGTACW	5 cut(s)	342, 699, 926, 1105, 1737
TauI	GCSGC	2 cut(s)	408, 1609
TfiI	GAWTC	6 cut(s)	655, 1397, 1694, 1838, 1912, 2275
TscAI	CASTG	5 cut(s)	517, 715, 985, 1188, 1982
TseFI	GTSAC	4 cut(s)	1219, 1852, 2140, 2258
TseI	GCWGC	6 cut(s)	839, 917, 1609, 2090, 2124, 2213
Tsp45I	GTSAC	4 cut(s)	1219, 1852, 2140, 2258
TspDTI	ATGAA	8 cut(s)	510, 572, 1064, 1331, 1437, 1455, 1806, 1907
TspGWI	ACGGA	4 cut(s)	328, 1025, 1058, 1701
TspRI	CASTG	5 cut(s)	517, 715, 985, 1188, 1982
Tth111I	GACNNNGTC	3 cut(s)	512, 594, 1217
Van91I	CCANNNNNTGG	1 cut(s)	990
VpaK11BI	GGWCC	2 cut(s)	590, 1544
VspI	ATTAAT	2 cut(s)	893, 1370
XapI	RAATTY	6 cut(s)	122, 544, 944, 1447, 1665, 1793
XceI	RCATGY	6 cut(s)	82, 293, 1018, 1119, 1474, 1998
XhoI	CTCGAG	1 cut(s)	1248
XmnI	GAANNNNTTC	2 cut(s)	282, 948
XspI	CTAG	1 cut(s)	720
ZrmI	AGTACT	1 cut(s)	1107
Zsp2I	ATGCAT	3 cut(s)	84, 1996, 2293

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.