RLG00000011094

Vacuolar protein sorting-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0012830
Physical Location & Seq
Forward (+)
6752377 .. 6758392
6016 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011094

Sequence Viewer

Length: 2373 bp
ATGCTACTGGACGGAATCGAAGACGAGGACAAATGGCTCGCAGAAGGCATCGCCGGCATTCAACACCACGCCTTCTACATGCATCGAGCTCTGGACGCGAACAATCTCAGAGACGCTCTCAAATACTCGGCTCAGATGCTCTCCGAGCTCCGAACCTCGAGGCTCTCGCCTCACAAATACTACGAACTCTATATGCGAGCGTTCGATGAGTTGAGGAAGCTGGAGATGTTCTTCAAGGACGAGAGCAGGCATGGCGTTTCGATCATTGATCTCTACGAGCTTGTTCAGCACGCTGGCAACATTTTACCGAGATTGTATCTCCTATGTACGGTAGGATCTGTGTACATTAAATCGAAGGAAGCACCTGCCAAGGACGTGCTTAAAGACCTTGTGGAAATGTGTCGTGCTGTTCAACATCCGATTCGGGGGCTCTTTCTAAGGAGTTACCTTTCTCAAGTCAGTAGAGATAAGTTACCTGATCTCGGTTCCGAGTATGAAGGAGGTTCTGACACTGTTACGGATGCCGTAGATTTTGTGCTACAGAATTTTACAGAGATGAATAAGCTTTGGGTTCGAATGCAGTATCAGGGACCTGGTCGGGCGAGAGAGAAGCATGAAAAGGAAAGAAGCGAACTTCGTGATCTAGTAGGGAAAAATCTCCATGTTCTCAGTCAGATAGAGGGTGTGGAACTCCAAATGTACAAAGACACTGTTCTTCCCAGAGTATTAGAGCAGGTTATCAACTGTAAAGATGAGCTGGCCCAGTATTATTTGATGGATTGCCTGATCCAGGTCTTTCCGGATGAATACCACTTGCAGACACTGGAGACGTTATTGGGTGCTTGCCCCCAGCTTCAGCCAACAGTGGATATCAAGACAGTGCTATCTCAATTAATGGACAGGTTATCAAATTATGCTGCTTCAAGTACAGATGTATTACCCGAATTTCTTCAAGTCGAAGCTTTCGCTAAATTAAGTAGTGCTATTGGGAGGGTGATAGAAGCACAAGTTGACATGCCTATTGTTGGAGCCATATCTTTGTACGTCTCTCTTCTTACATTCACACTCCGTGTTCATCCTGATCGTCTAGATTATGTGGATCAAGTACTGGGAGCATGTGTTAGGAAGCTACCCGGAAACACTAAGGTTGAAGACAAGAGAGCCATAAAGCAGGTTGTCGCACTATTAAGTGCTCCTTTGGAAAAGTACAATGACATTGTCACAGCCCTCACACTTTCTAATTATCCTCGAGTTATGGATCACCTTGATAATGGAACAAATAAAGTCATGGCAATGGTTATCATTCAAAGCATTATGAAAAACGACTCTTGTATCTCCACTGCTGATAAGGTGGAGGTATTGTTTGAATTAATCAAAGGGCTGATCAAGGATTTGGATGGTACTTCTGATGATGAGCTTGACGAGGAGGATTTTCATGATGAGCAAAATTCTGTTGCTCGCCTTATACACATGCTTTATAATGATGATCCAGAAGAAATGTTAAAGATCATATGTACGGTAAAGAAGCACATAATGAATGGAGGGCCAAAGCGCTTACCTTTTACTGTTCCTCCTCTTGTATTTTCTGCACTTGGGTTGGTTAGGCGACTGCAAGGTCAGGATGGAGAGGTAGTTGGAGAAGATATGCCTGCCACACCGAAGACAGTTTTTCAGTTTCTAAATCAGACTGTAGACGCTCTTTCCTCTATTCCGTCTCCTGAACTGGCATTAAGGCTGTACTTACATTGCGCTGAGGCTGCCAACGACTGTGATCTTGAACCTGTTGCGTATGAATTCTTCACTCAGGCATTTGTACTATATGAAGAAGAAATCGCGGATTCCAAAGCTCAAGTGACTGCAATACATCTCATTATTGGGGCTCTTCAGAGGATGAATGTGTTTGGCACTGAAAATAGGGATACTTTAACACACAAGGCCACAGGGTATTCTGCAAAGCTTTTGAAGAAACCTGACCAGTGCAGAGCAGTGTATGCATGTTCACACCTGTTTTGGGTTGATGATCAGGATGGTGTTAAAGATGGAGAAAGGGTCTTGCTTTGTTTAAAGCGTGCCTTGAGAATTGCAAATGCTGCTCAACAAATGGTCAGTGTAACTCGGGGTAGCAGTGGGCCAGTGACACTCTTTGTTGAAATACTAAACAAGTACCTCTACTTTTTTGAGAAAGGAAACCCCCAGATTACCAGTGCTGCAATTCAGGGATTGGTGGAGTTAATTACAACCGAGTTGCAAAGCGACTCTAGTAATGTGAAACCAGCCTCTGATGCATTTTTTGCCAGCACATTGCGGTACATACAGTTCCAGAAACAGAAAGGCGGGGCAATGGGTGAAAAATATGCATCTATCAAGGTCTGA

Protein Analysis

791

Amino Acids

88.98

Weight (kDa)

5.35

Isoelectric Point (pI)

38.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps35 PF03635 12 - 747 2.7e-289 Vacuolar protein sorting-associated protein 35
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012830)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75850 AT1G75850
fragaria_vesca FvH4_2g38180 FvH4_2g38180
malus_domestica MD15G1010200.v1.1
prunus_persica Prupe.1G362700_v2.0.a1
pyrus_communis pycom15g00840
rosa_chinensis RchiOBHm_Chr6g0303301
rosa_laevigata RLG00000011094
rosa_multiflora Rmu_sc0006346.1_g000001 Rmu_sc0019430.1_g000001
rosa_roxburghii Rroxscaffold_7G00164890
rosa_rugosa Rorug06G0320200
rosa_samantha Rh6AG434600 Rh6BG468500 Rh6CG446600 Rh6DG433300
rosa_wichuraiana Rw6G037630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1479
AarI CACCTGC 1 cut(s) 373
AasI GACNNNNNNGTC 1 cut(s) 1614
AbsI CCTCGAGG 1 cut(s) 157
Acc36I ACCTGC 3 cut(s) 373, 724, 1162
AccI GTMKAC 1 cut(s) 1692
AccII CGCG 2 cut(s) 98, 1835
AccIII TCCGGA 1 cut(s) 799
AciI CCGC 3 cut(s) 1835, 2305, 2334
AclWI GGATC 5 cut(s) 343, 781, 1107, 1266, 1481
AcsI RAATTY 4 cut(s) 544, 944, 1447, 1793
AcuI CTGAAG 2 cut(s) 839, 1868
AdeI CACNNNGTG 1 cut(s) 1070
AfeI AGCGCT 1 cut(s) 1553
AfiI CCNNNNNNNGG 6 cut(s) 328, 425, 482, 790, 1025, 2011
AjiI CACGTC 1 cut(s) 376
AjnI CCWGG 2 cut(s) 592, 789
Alw21I GWGCWC 3 cut(s) 91, 150, 1195
Alw26I GTCTC 4 cut(s) 105, 821, 1051, 1719
AlwI GGATC 5 cut(s) 343, 781, 1107, 1266, 1481
AlwNI CAGNNNCTG 2 cut(s) 823, 2279
Ama87I CYCGRG 3 cut(s) 157, 1248, 2115
Aor13HI TCCGGA 1 cut(s) 799
Aor51HI AGCGCT 1 cut(s) 1553
AoxI GGCC 4 cut(s) 759, 1544, 1935, 2129
ApeKI GCWGC 4 cut(s) 917, 1757, 2090, 2207
ApoI RAATTY 4 cut(s) 544, 944, 1447, 1793
AseI ATTAAT 2 cut(s) 893, 1370
Asp700I GAANNNNTTC 1 cut(s) 948
AspLEI GCGC 2 cut(s) 1554, 1751
AspS9I GGNCC 4 cut(s) 590, 760, 1544, 2129
AsuC2I CCSGG 1 cut(s) 1134
AsuHPI GGTGA 3 cut(s) 1006, 1253, 2357
AsuII TTCGAA 1 cut(s) 574
AvaI CYCGRG 3 cut(s) 157, 1248, 2115
AvaII GGWCC 1 cut(s) 590
BaeI ACNNNNGTAYC 2 cut(s) 1315, 1348
BanII GRGCYC 4 cut(s) 91, 150, 432, 1882
BarI GAAGNNNNNNTAC 2 cut(s) 1781, 1813
BbsI GAAGAC 3 cut(s) 27, 1158, 1667
Bbv12I GWGCWC 3 cut(s) 91, 150, 1195
BbvCI CCTCAGC 1 cut(s) 1752
BbvI GCAGC 4 cut(s) 904, 1744, 2077, 2194
BccI CCATC 5 cut(s) 769, 1391, 1616, 2021, 2033
BceAI ACGGC 1 cut(s) 509
BciT130I CCWGG 2 cut(s) 594, 791
BciVI GTATCC 1 cut(s) 1912
BclI TGATCA 2 cut(s) 1383, 2020
BcnI CCSGG 1 cut(s) 1134
BcoDI GTCTC 4 cut(s) 105, 821, 1051, 1719
BfaI CTAG 3 cut(s) 644, 1088, 2259
BfmI CTRYAG 2 cut(s) 539, 1689
BfoI RGCGCY 1 cut(s) 1555
BfuAI ACCTGC 3 cut(s) 373, 724, 1162
BfuI GTATCC 1 cut(s) 1912
BisI GCNGC 4 cut(s) 918, 1758, 2091, 2208
BlsI GCNGC 4 cut(s) 919, 1759, 2092, 2209
BmcAI AGTACT 1 cut(s) 1107
Bme1390I CCNGG 3 cut(s) 594, 791, 1134
Bme18I GGWCC 1 cut(s) 590
BmeT110I CYCGRG 3 cut(s) 157, 1248, 2115
BmgBI CACGTC 1 cut(s) 376
BmgT120I GGNCC 4 cut(s) 590, 760, 1544, 2129
BmiI GGNNCC 3 cut(s) 487, 591, 1030
BmrFI CCNGG 3 cut(s) 594, 791, 1134
BmrI ACTGGG 2 cut(s) 757, 1118
BmsI GCATC 6 cut(s) 57, 91, 126, 511, 2272, 2366
BmuI ACTGGG 2 cut(s) 757, 1118
BpiI GAAGAC 3 cut(s) 27, 1158, 1667
BplI GAGNNNNNCTC 2 cut(s) 102, 134
BpmI CTGGAG 2 cut(s) 242, 845
Bpu10I CCTNAGC 1 cut(s) 1752
Bpu14I TTCGAA 1 cut(s) 574
BpuEI CTTGAG 3 cut(s) 438, 1833, 2095
BpuMI CCSGG 1 cut(s) 1134
BsaJI CCNNGG 1 cut(s) 369
BsaWI WCCGGW 1 cut(s) 799
Bsc4I CCNNNNNNNGG 6 cut(s) 328, 425, 482, 790, 1025, 2011
Bse118I RCCGGY 1 cut(s) 53
Bse1I ACTGG 8 cut(s) 12, 763, 828, 1113, 1728, 1975, 2132, 2202
Bse3DI GCAATG 4 cut(s) 1299, 1744, 2300, 2346
BseAI TCCGGA 1 cut(s) 799
BseBI CCWGG 2 cut(s) 594, 791
BseDI CCNNGG 1 cut(s) 369
BseGI GGATG 8 cut(s) 415, 526, 808, 1075, 1402, 1627, 1896, 2032
BseLI CCNNNNNNNGG 6 cut(s) 328, 425, 482, 790, 1025, 2011
BseMI GCAATG 4 cut(s) 1299, 1744, 2300, 2346
BseMII CTCAG 5 cut(s) 121, 146, 682, 1743, 1817
BseNI ACTGG 8 cut(s) 12, 763, 828, 1113, 1728, 1975, 2132, 2202
BseRI GAGGAG 2 cut(s) 1439, 1563
BseXI GCAGC 4 cut(s) 904, 1744, 2077, 2194
BseYI CCCAGC 1 cut(s) 849
BsgI GTGCAG 2 cut(s) 1572, 1999
Bsh1236I CGCG 2 cut(s) 98, 1835
BshFI GGCC 4 cut(s) 761, 1546, 1937, 2131
BsiHKAI GWGCWC 3 cut(s) 91, 150, 1195
BsiHKCI CYCGRG 3 cut(s) 157, 1248, 2115
BsiSI CCGG 3 cut(s) 54, 800, 1134
BslFI GGGAC 1 cut(s) 603
BslI CCNNNNNNNGG 6 cut(s) 328, 425, 482, 790, 1025, 2011
BsmAI GTCTC 4 cut(s) 105, 821, 1051, 1719
BsmBI CGTCTC 4 cut(s) 105, 821, 1051, 1719
BsmFI GGGAC 1 cut(s) 603
BsmI GAATGC 2 cut(s) 57, 582
BsnI GGCC 4 cut(s) 761, 1546, 1937, 2131
BsoBI CYCGRG 3 cut(s) 157, 1248, 2115
Bsp119I TTCGAA 1 cut(s) 574
Bsp1286I GDGCHC 5 cut(s) 91, 150, 432, 1195, 1882
Bsp13I TCCGGA 1 cut(s) 799
Bsp1407I TGTACA 2 cut(s) 342, 699
BspACI CCGC 3 cut(s) 1835, 2305, 2334
BspANI GGCC 4 cut(s) 761, 1546, 1937, 2131
BspCNI CTCAG 5 cut(s) 120, 145, 681, 1744, 1816
BspEI TCCGGA 1 cut(s) 799
BspFNI CGCG 2 cut(s) 98, 1835
BspHI TCATGA 1 cut(s) 1435
BspLI GGNNCC 3 cut(s) 487, 591, 1030
BspMI ACCTGC 3 cut(s) 373, 724, 1162
BspPI GGATC 5 cut(s) 343, 781, 1107, 1266, 1481
BspQI GCTCTTC 1 cut(s) 1887
BspT104I TTCGAA 1 cut(s) 574
BsrDI GCAATG 4 cut(s) 1299, 1744, 2300, 2346
BsrFI RCCGGY 1 cut(s) 53
BsrGI TGTACA 2 cut(s) 342, 699
BsrI ACTGG 8 cut(s) 12, 763, 828, 1113, 1728, 1975, 2132, 2202
BssAI RCCGGY 1 cut(s) 53
BssECI CCNNGG 1 cut(s) 369
BssT1I CCWWGG 1 cut(s) 369
Bst2UI CCWGG 2 cut(s) 594, 791
Bst6I CTCTTC 2 cut(s) 1056, 1887
BstAPI GCANNNNNTGC 3 cut(s) 1991, 2090, 2291
BstAUI TGTACA 2 cut(s) 342, 699
BstBI TTCGAA 1 cut(s) 574
BstDEI CTNAG 7 cut(s) 107, 132, 437, 668, 1143, 1752, 1803
BstENI CCTNNNNNAGG 1 cut(s) 788
BstF5I GGATG 8 cut(s) 415, 526, 808, 1075, 1402, 1627, 1896, 2032
BstFNI CGCG 2 cut(s) 98, 1835
BstH2I RGCGCY 1 cut(s) 1555
BstHHI GCGC 2 cut(s) 1554, 1751
BstMAI GTCTC 4 cut(s) 105, 821, 1051, 1719
BstNI CCWGG 2 cut(s) 594, 791
BstNSI RCATGY 5 cut(s) 82, 1018, 1119, 1474, 1998
BstSCI CCNGG 3 cut(s) 592, 789, 1132
BstSFI CTRYAG 2 cut(s) 539, 1689
BstUI CGCG 2 cut(s) 98, 1835
BstV1I GCAGC 4 cut(s) 904, 1744, 2077, 2194
BstV2I GAAGAC 3 cut(s) 27, 1158, 1667
BstX2I RGATCY 1 cut(s) 335
BstYI RGATCY 1 cut(s) 335
BsuI GTATCC 1 cut(s) 1912
BsuRI GGCC 4 cut(s) 761, 1546, 1937, 2131
BtgZI GCGATG 1 cut(s) 34
BtrI CACGTC 1 cut(s) 376
BtsCI GGATG 8 cut(s) 415, 526, 808, 1075, 1402, 1627, 1896, 2032
BtsI GCAGTG 3 cut(s) 1338, 1992, 2131
BveI ACCTGC 3 cut(s) 373, 724, 1162
CaiI CAGNNNCTG 2 cut(s) 823, 2279
CciI TCATGA 1 cut(s) 1435
CfoI GCGC 2 cut(s) 1554, 1751
Cfr10I RCCGGY 1 cut(s) 53
Cfr13I GGNCC 4 cut(s) 590, 760, 1544, 2129
CseI GACGC 3 cut(s) 104, 122, 1703
CsiI ACCWGGT 1 cut(s) 592
DdeI CTNAG 7 cut(s) 107, 132, 437, 668, 1143, 1752, 1803
DraI TTTAAA 1 cut(s) 2064
DraIII CACNNNGTG 1 cut(s) 1070
DrdI GACNNNNNNGTC 1 cut(s) 1614
DseDI GACNNNNNNGTC 1 cut(s) 1614
Eam1104I CTCTTC 2 cut(s) 1056, 1887
EarI CTCTTC 2 cut(s) 1056, 1887
Ecl136II GAGCTC 2 cut(s) 89, 148
Eco130I CCWWGG 1 cut(s) 369
Eco24I GRGCYC 4 cut(s) 91, 150, 432, 1882
Eco32I GATATC 1 cut(s) 871
Eco47I GGWCC 1 cut(s) 590
Eco47III AGCGCT 1 cut(s) 1553
Eco53kI GAGCTC 2 cut(s) 89, 148
Eco57I CTGAAG 2 cut(s) 839, 1868
Eco88I CYCGRG 3 cut(s) 157, 1248, 2115
EcoICRI GAGCTC 2 cut(s) 89, 148
EcoNI CCTNNNNNAGG 1 cut(s) 788
EcoO109I RGGNCCY 1 cut(s) 590
EcoRI GAATTC 1 cut(s) 1793
EcoRII CCWGG 2 cut(s) 592, 789
EcoRV GATATC 1 cut(s) 871
EcoT14I CCWWGG 1 cut(s) 369
EcoT22I ATGCAT 4 cut(s) 84, 1996, 2287, 2359
EcoT38I GRGCYC 4 cut(s) 91, 150, 432, 1882
ErhI CCWWGG 1 cut(s) 369
Esp3I CGTCTC 4 cut(s) 105, 821, 1051, 1719
FalI AAGNNNNNCTT 4 cut(s) 1180, 1212, 2057, 2089
FaqI GGGAC 1 cut(s) 603
FauI CCCGC 1 cut(s) 2327
FauNDI CATATG 1 cut(s) 1511
FbaI TGATCA 2 cut(s) 1383, 2020
FblI GTMKAC 1 cut(s) 1692
Fnu4HI GCNGC 4 cut(s) 918, 1758, 2091, 2208
FokI GGATG 8 cut(s) 402, 533, 815, 1062, 1409, 1634, 1903, 2039
FriOI GRGCYC 4 cut(s) 91, 150, 432, 1882
Fsp4HI GCNGC 4 cut(s) 918, 1758, 2091, 2208
FspBI CTAG 3 cut(s) 644, 1088, 2259
GlaI GCGC 2 cut(s) 1553, 1750
GluI GCNGC 4 cut(s) 918, 1758, 2091, 2208
GsaI CCCAGC 1 cut(s) 853
GsuI CTGGAG 2 cut(s) 242, 845
HaeII RGCGCY 1 cut(s) 1555
HaeIII GGCC 4 cut(s) 761, 1546, 1937, 2131
HapII CCGG 3 cut(s) 54, 800, 1134
HgaI GACGC 3 cut(s) 104, 122, 1703
HhaI GCGC 2 cut(s) 1554, 1751
Hin6I GCGC 2 cut(s) 1552, 1749
HinP1I GCGC 2 cut(s) 1552, 1749
HincII GTYRAC 1 cut(s) 1012
HindII GTYRAC 1 cut(s) 1012
HindIII AAGCTT 3 cut(s) 563, 960, 1955
HinfI GANTC 5 cut(s) 15, 421, 1325, 1838, 2255
HpaII CCGG 3 cut(s) 54, 800, 1134
HphI GGTGA 3 cut(s) 1006, 1253, 2357
Hpy166II GTNNAC 4 cut(s) 343, 1012, 1693, 2000
Hpy8I GTNNAC 4 cut(s) 343, 1012, 1693, 2000
HpyAV CCTTC 4 cut(s) 38, 82, 349, 491
HpyCH4IV ACGT 3 cut(s) 375, 830, 1044
HpyF3I CTNAG 7 cut(s) 107, 132, 437, 668, 1143, 1752, 1803
HpySE526I ACGT 3 cut(s) 375, 830, 1044
HspAI GCGC 2 cut(s) 1552, 1749
Kpn2I TCCGGA 1 cut(s) 799
KroI GCCGGC 1 cut(s) 53
KroNI GCCGGC 1 cut(s) 55
Ksp22I TGATCA 2 cut(s) 1383, 2020
LguI GCTCTTC 1 cut(s) 1887
LmnI GCTCC 4 cut(s) 153, 1028, 1112, 1198
Lsp1109I GCAGC 4 cut(s) 904, 1744, 2077, 2194
LweI GCATC 6 cut(s) 57, 91, 126, 511, 2272, 2366
MabI ACCWGGT 1 cut(s) 592
MaeI CTAG 3 cut(s) 644, 1088, 2259
MaeII ACGT 3 cut(s) 375, 830, 1044
MaeIII GTNAC 7 cut(s) 443, 471, 514, 1219, 1852, 2110, 2134
MflI RGATCY 1 cut(s) 335
MhlI GDGCHC 5 cut(s) 91, 150, 432, 1195, 1882
MlyI GAGTC 2 cut(s) 1319, 2249
MmeI TCCRAC 2 cut(s) 1006, 1615
Mph1103I ATGCAT 4 cut(s) 84, 1996, 2287, 2359
MroI TCCGGA 1 cut(s) 799
MroNI GCCGGC 1 cut(s) 53
MroXI GAANNNNTTC 1 cut(s) 948
MslI CAYNNNNRTG 1 cut(s) 1292
MspI CCGG 3 cut(s) 54, 800, 1134
MspR9I CCNGG 3 cut(s) 594, 791, 1134
Mva1269I GAATGC 2 cut(s) 57, 582
MvaI CCWGG 2 cut(s) 594, 791
MvnI CGCG 2 cut(s) 98, 1835
NaeI GCCGGC 1 cut(s) 55
NciI CCSGG 1 cut(s) 1134
NdeI CATATG 1 cut(s) 1511
NgoMIV GCCGGC 1 cut(s) 53
NlaIV GGNNCC 3 cut(s) 487, 591, 1030
NmeAIII GCCGAG 1 cut(s) 107
NmuCI GTSAC 3 cut(s) 1219, 1852, 2134
NsiI ATGCAT 4 cut(s) 84, 1996, 2287, 2359
NspI RCATGY 5 cut(s) 82, 1018, 1119, 1474, 1998
NspV TTCGAA 1 cut(s) 574
PaeR7I CTCGAG 2 cut(s) 157, 1248
PagI TCATGA 1 cut(s) 1435
PaqCI CACCTGC 1 cut(s) 373
PciSI GCTCTTC 1 cut(s) 1887
PctI GAATGC 2 cut(s) 57, 582
PdiI GCCGGC 1 cut(s) 55
PdmI GAANNNNTTC 1 cut(s) 948
PfeI GAWTC 3 cut(s) 15, 421, 1838
PflFI GACNNNGTC 2 cut(s) 594, 1217
PkrI GCNGC 4 cut(s) 919, 1759, 2092, 2209
PleI GAGTC 2 cut(s) 1319, 2249
PpsI GAGTC 2 cut(s) 1319, 2249
PpuMI RGGWCCY 1 cut(s) 590
PshBI ATTAAT 2 cut(s) 893, 1370
PsiI TTATAA 1 cut(s) 1479
Psp124BI GAGCTC 2 cut(s) 91, 150
Psp5II RGGWCCY 1 cut(s) 590
Psp6I CCWGG 2 cut(s) 592, 789
PspFI CCCAGC 1 cut(s) 849
PspGI CCWGG 2 cut(s) 592, 789
PspN4I GGNNCC 3 cut(s) 487, 591, 1030
PspPI GGNCC 4 cut(s) 590, 760, 1544, 2129
PspPPI RGGWCCY 1 cut(s) 590
PspXI VCTCGAGB 2 cut(s) 157, 1248
PsrI GAACNNNNNNTAC 2 cut(s) 2300, 2332
PstNI CAGNNNCTG 2 cut(s) 823, 2279
PsuI RGATCY 1 cut(s) 335
PsyI GACNNNGTC 2 cut(s) 594, 1217
RseI CAYNNNNRTG 1 cut(s) 1292
SacI GAGCTC 2 cut(s) 91, 150
SapI GCTCTTC 1 cut(s) 1887
SatI GCNGC 4 cut(s) 918, 1758, 2091, 2208
Sau96I GGNCC 4 cut(s) 590, 760, 1544, 2129
ScaI AGTACT 1 cut(s) 1107
SchI GAGTC 2 cut(s) 1319, 2249
ScrFI CCNGG 3 cut(s) 594, 791, 1134
SduI GDGCHC 5 cut(s) 91, 150, 432, 1195, 1882
SexAI ACCWGGT 1 cut(s) 592
SfaNI GCATC 6 cut(s) 57, 91, 126, 511, 2272, 2366
SfcI CTRYAG 2 cut(s) 539, 1689
Sfr274I CTCGAG 2 cut(s) 157, 1248
SfuI TTCGAA 1 cut(s) 574
SinI GGWCC 1 cut(s) 590
SlaI CTCGAG 2 cut(s) 157, 1248
SmiMI CAYNNNNRTG 1 cut(s) 1292
SmlI CTYRAG 5 cut(s) 157, 453, 1248, 1848, 2074
SmoI CTYRAG 5 cut(s) 157, 453, 1248, 1848, 2074
SsiI CCGC 3 cut(s) 1835, 2305, 2334
SspMI CTAG 3 cut(s) 644, 1088, 2259
SstI GAGCTC 2 cut(s) 91, 150
StyD4I CCNGG 3 cut(s) 592, 789, 1132
StyI CCWWGG 1 cut(s) 369
TaiI ACGT 3 cut(s) 378, 833, 1047
TaqI TCGA 9 cut(s) 18, 85, 158, 204, 260, 353, 574, 957, 1249
TatI WGTACW 7 cut(s) 342, 699, 926, 1105, 1206, 1737, 1813
TfiI GAWTC 3 cut(s) 15, 421, 1838
TseFI GTSAC 3 cut(s) 1219, 1852, 2134
TseI GCWGC 4 cut(s) 917, 1757, 2090, 2207
Tsp45I GTSAC 3 cut(s) 1219, 1852, 2134
TspGWI ACGGA 4 cut(s) 27, 533, 1058, 1701
Tth111I GACNNNGTC 2 cut(s) 594, 1217
VpaK11BI GGWCC 1 cut(s) 590
VspI ATTAAT 2 cut(s) 893, 1370
XagI CCTNNNNNAGG 1 cut(s) 788
XapI RAATTY 4 cut(s) 544, 944, 1447, 1793
XbaI TCTAGA 1 cut(s) 1087
XceI RCATGY 5 cut(s) 82, 1018, 1119, 1474, 1998
XhoI CTCGAG 2 cut(s) 157, 1248
XmiI GTMKAC 1 cut(s) 1692
XmnI GAANNNNTTC 1 cut(s) 948
XspI CTAG 3 cut(s) 644, 1088, 2259
ZrmI AGTACT 1 cut(s) 1107
Zsp2I ATGCAT 4 cut(s) 84, 1996, 2287, 2359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.