Rw6G037630

Vacuolar protein sorting-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0012830
Physical Location & Seq
Reverse (-)
61908724 .. 61914721
5998 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G037630.1

Sequence Viewer

Length: 2301 bp
ATGCTACTGGACGGAATCGAAGACGAGGACAAATGGCTCGCAGAAGGCATCGCCGGCATTCAACACCACGCCTTCTACATGCATCGAGCTCTGGACGCTAACAATCTCAGAGACGCTCTCAAATACTCGGCTCAGATGCTCTCCGAGCTCCGAACCTCGAGGCTCTCGCCTCACAAATACTACGAACTCTATATGCGAGCGTTCGATGAGTTGAGGAAGCTGGAGATGTTCTTCAAGGACGAGAGCAGGCATGGCGTTTCGATCATTGATCTCTACGAGCTTGTTCAGCACGCTGGCAACATTCTACCGAGATTGTATCTCCTATGTACGGTAGGATCTGTGTACATTAAATCAAAGGAAGCACCTGCCAAAGACGTGCTTAAAGACCTTGTGGAAATGTGTCGTGCCGTTCAACATCCGATTCGGGGGCTCTTTCTGAGGAGTTACCTTTCTCAAGTCAGTAGAGATAAGTTACCTGATCTCGGTTCCGAGTATGAAGGAGGTTCTGACACTGTTACGGATGCCGTAGATTTTGTGCTACAGAATTTTACAGAGATGAATAAGCTTTGGGTTCGAATGCAGTATCAGGGACCTGGTCGGGCGAGAGAGAAGCATGAAAAGGAAAGAAGCGAACTTCGTGATCTAGTAGGGAAAAATCTCCTTGTTCTCAGTCAGATAGAGGGTGTGGAACTCCAAATGTACAAAGACACTGTTCTTCCCAGAGTATTAGAGCAGGTTATCAACTGTAAAGATGAGCTGGCCCAGTATTATTTGATGGATTGCCTGATCCAGGTCTTTCCGGATGAGTACCACTTGCAGACACTGGAGACATTATTGGGTGCTTGCCCCCAGCTTCAGCCAACAGTGGATATCAAGACAGTGCTATCTCAATTAATGGACAGGTTATCAAATTATGCTGCTTCAAGTGCAGATGTATTACCCGAATTTCTTCAAGTCGAAGCTTTCGCTAAATTAAGTAGTGCTATTGGGAGGGTGATAGAAGCACAAGTTGACATGCCTATTGTTGGAGCCATATCTTTGTACGTCTCTCTTCTTACATTCACACTCCGTGGAGCATGTGTTAGGAAGCTACCGGGAAACACTAAGGTTGAAGACAAGAGAGCCATAAAGCAGGTTGTCGCACTATTAAGTGCTCCTTTGGAAAAGTACAATGACATTGTCACAGCCCTCACACTTTCTAATTATCCTCGAGTTATGGATCACCTTGATAATGGAACAAATAAAGTCATGGCAATGGTTATCATTCAAAGCATTATGAAAAACGACTCTTGTATCTCCACTGCTGATAAGGTGGAGGTATTGTTTGAATTAATCAAAGGGCTGATCAAGGATTTGGATGGTACTTCTGATGATGAGCTTGACGAGGAGGATTTTCATGATGAGCAAAATTCTGTTGCTCGCCTTATACACATGCTTTATAATGATGATCCAGAAGAAATGTTAAAGATCATATGTACGGTAAAGAAGCACATAATGAATGGAGGGCCAAGGCGCTTACCTTTTACTGTTCCTCCTCTTGTTTTTTCTGCACTTGGGGCGACTGCAAGGTCAGATGGAGAGGTAGTTGGAGAAGATATGCCTGCCACGCCGAAGACAGTTTTTCAGTTTCTAAATCAGACTGTAGACGCTCTTTCCTCTATTCCGTCTCCTGAACTGGCATTAAGGCTGTACTTACATTGTGCTGAGGCTGCCAACGACTGTGATCTTGAACCTGTTGCTTATGAATTCTTCACTCAGGCATTTGTACTATATGAAGAAGAAATCGCGGATTCCAAAGCTCAAGTGACTGCAATACATCTCATTATTGGGGCTCTTCAGAGGATGAATGTGTTTGGCACTGAAAATAGGGATACTTTAACACACAAGGCCACAGGGTATTCTGCAAAGCTTTTGAAGAAACCTGACCAGTGCAGAGCAGTGTATGCATGTTCACACCTGTTTTGGGTTGATGATCAGGATGGTGTTAAAGATGGAGAAAGGGTCTTGCTTTGTTTAAAGCGTGCCTTGAGAATTGCAAATGCTGCTCAACAAATGGCCAGTGTAACTCGGGGTAGCAGTGGGCCAGTGACACTCTTTGTTGAAATACTAAACAAGTACCTCTACTTTTTTGAGAAAGGAAACCCCCAGATTACCAGTGCTGCAATTCAGGGATTGGTGGAGTTAATTACAACCGAGTTGCAAAGTGACTCTAGTAATGTGAAACCAGCCTCTGATGCATTTTTTGCCAGCACATTGCGGTACATACAGTTCCAGAAAACAGAAAGGCGGGGCAATGGGTGA

Protein Analysis

766

Amino Acids

86.13

Weight (kDa)

5.28

Isoelectric Point (pI)

39.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps35 PF03635 12 - 731 2e-273 Vacuolar protein sorting-associated protein 35
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012830)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75850 AT1G75850
fragaria_vesca FvH4_2g38180 FvH4_2g38180
malus_domestica MD15G1010200.v1.1
prunus_persica Prupe.1G362700_v2.0.a1
pyrus_communis pycom15g00840
rosa_chinensis RchiOBHm_Chr6g0303301
rosa_laevigata RLG00000011094
rosa_multiflora Rmu_sc0006346.1_g000001 Rmu_sc0019430.1_g000001
rosa_roxburghii Rroxscaffold_7G00164890
rosa_rugosa Rorug06G0320200
rosa_samantha Rh6AG434600 Rh6BG468500 Rh6CG446600 Rh6DG433300
rosa_wichuraiana Rw6G037630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1440
AarI CACCTGC 1 cut(s) 373
AasI GACNNNNNNGTC 1 cut(s) 1567
AbsI CCTCGAGG 1 cut(s) 157
Acc36I ACCTGC 3 cut(s) 373, 724, 1123
AccI GTMKAC 1 cut(s) 1644
AccII CGCG 1 cut(s) 1787
AccIII TCCGGA 1 cut(s) 799
AciI CCGC 3 cut(s) 1787, 2257, 2287
AclWI GGATC 4 cut(s) 343, 781, 1227, 1442
AcoI YGGCCR 1 cut(s) 2055
AcsI RAATTY 4 cut(s) 544, 944, 1408, 1745
AcuI CTGAAG 2 cut(s) 839, 1820
AdeI CACNNNGTG 1 cut(s) 1070
AfiI CCNNNNNNNGG 6 cut(s) 328, 425, 482, 790, 1025, 1963
AjiI CACGTC 1 cut(s) 376
AjnI CCWGG 2 cut(s) 592, 789
Alw21I GWGCWC 3 cut(s) 91, 150, 1156
Alw26I GTCTC 4 cut(s) 105, 821, 1051, 1671
AlwI GGATC 4 cut(s) 343, 781, 1227, 1442
AlwNI CAGNNNCTG 2 cut(s) 823, 2231
Ama87I CYCGRG 3 cut(s) 157, 1209, 2067
Aor13HI TCCGGA 1 cut(s) 799
AoxI GGCC 5 cut(s) 759, 1505, 1887, 2055, 2081
ApeKI GCWGC 4 cut(s) 917, 1709, 2042, 2159
ApoI RAATTY 4 cut(s) 544, 944, 1408, 1745
AseI ATTAAT 2 cut(s) 893, 1331
Asp700I GAANNNNTTC 1 cut(s) 948
AspLEI GCGC 1 cut(s) 1515
AspS9I GGNCC 4 cut(s) 590, 760, 1505, 2081
AsuC2I CCSGG 1 cut(s) 1095
AsuHPI GGTGA 2 cut(s) 1006, 1214
AsuII TTCGAA 1 cut(s) 574
AvaI CYCGRG 3 cut(s) 157, 1209, 2067
AvaII GGWCC 1 cut(s) 590
BaeI ACNNNNGTAYC 2 cut(s) 1276, 1309
BalI TGGCCA 1 cut(s) 2057
BanII GRGCYC 4 cut(s) 91, 150, 432, 1834
BbsI GAAGAC 3 cut(s) 27, 1119, 1619
Bbv12I GWGCWC 3 cut(s) 91, 150, 1156
BbvCI CCTCAGC 1 cut(s) 1704
BbvI GCAGC 4 cut(s) 904, 1696, 2029, 2146
BccI CCATC 5 cut(s) 769, 1352, 1568, 1973, 1985
BceAI ACGGC 2 cut(s) 392, 509
BciT130I CCWGG 2 cut(s) 594, 791
BciVI GTATCC 1 cut(s) 1864
BclI TGATCA 2 cut(s) 1344, 1972
BcnI CCSGG 1 cut(s) 1095
BcoDI GTCTC 4 cut(s) 105, 821, 1051, 1671
BfaI CTAG 2 cut(s) 644, 2211
BfmI CTRYAG 2 cut(s) 539, 1641
BfoI RGCGCY 1 cut(s) 1516
BfuAI ACCTGC 3 cut(s) 373, 724, 1123
BfuI GTATCC 1 cut(s) 1864
BisI GCNGC 4 cut(s) 918, 1710, 2043, 2160
BlsI GCNGC 4 cut(s) 919, 1711, 2044, 2161
Bme1390I CCNGG 3 cut(s) 594, 791, 1095
Bme18I GGWCC 1 cut(s) 590
BmeT110I CYCGRG 3 cut(s) 157, 1209, 2067
BmgBI CACGTC 1 cut(s) 376
BmgT120I GGNCC 4 cut(s) 590, 760, 1505, 2081
BmiI GGNNCC 3 cut(s) 487, 591, 1030
BmrFI CCNGG 3 cut(s) 594, 791, 1095
BmrI ACTGGG 1 cut(s) 757
BmsI GCATC 5 cut(s) 57, 91, 126, 511, 2224
BmuI ACTGGG 1 cut(s) 757
BpiI GAAGAC 3 cut(s) 27, 1119, 1619
BplI GAGNNNNNCTC 2 cut(s) 102, 134
BpmI CTGGAG 2 cut(s) 242, 845
Bpu10I CCTNAGC 1 cut(s) 1704
Bpu14I TTCGAA 1 cut(s) 574
BpuEI CTTGAG 3 cut(s) 438, 1785, 2047
BpuMI CCSGG 1 cut(s) 1095
BsaJI CCNNGG 2 cut(s) 1069, 1508
BsaWI WCCGGW 1 cut(s) 799
Bsc4I CCNNNNNNNGG 6 cut(s) 328, 425, 482, 790, 1025, 1963
Bse118I RCCGGY 1 cut(s) 53
Bse1I ACTGG 8 cut(s) 12, 763, 828, 1680, 1927, 2058, 2084, 2154
Bse3DI GCAATG 3 cut(s) 1260, 2252, 2299
BseAI TCCGGA 1 cut(s) 799
BseBI CCWGG 2 cut(s) 594, 791
BseDI CCNNGG 2 cut(s) 1069, 1508
BseGI GGATG 6 cut(s) 415, 526, 808, 1363, 1848, 1984
BseLI CCNNNNNNNGG 6 cut(s) 328, 425, 482, 790, 1025, 1963
BseMI GCAATG 3 cut(s) 1260, 2252, 2299
BseMII CTCAG 6 cut(s) 121, 146, 428, 682, 1695, 1769
BseNI ACTGG 8 cut(s) 12, 763, 828, 1680, 1927, 2058, 2084, 2154
BseRI GAGGAG 3 cut(s) 454, 1400, 1524
BseXI GCAGC 4 cut(s) 904, 1696, 2029, 2146
BseYI CCCAGC 1 cut(s) 849
BsgI GTGCAG 3 cut(s) 948, 1533, 1951
Bsh1236I CGCG 1 cut(s) 1787
BshFI GGCC 5 cut(s) 761, 1507, 1889, 2057, 2083
BsiHKAI GWGCWC 3 cut(s) 91, 150, 1156
BsiHKCI CYCGRG 3 cut(s) 157, 1209, 2067
BsiSI CCGG 3 cut(s) 54, 800, 1094
BslFI GGGAC 1 cut(s) 603
BslI CCNNNNNNNGG 6 cut(s) 328, 425, 482, 790, 1025, 1963
BsmAI GTCTC 4 cut(s) 105, 821, 1051, 1671
BsmBI CGTCTC 3 cut(s) 105, 1051, 1671
BsmFI GGGAC 1 cut(s) 603
BsmI GAATGC 2 cut(s) 57, 582
BsnI GGCC 5 cut(s) 761, 1507, 1889, 2057, 2083
BsoBI CYCGRG 3 cut(s) 157, 1209, 2067
Bsp119I TTCGAA 1 cut(s) 574
Bsp1286I GDGCHC 5 cut(s) 91, 150, 432, 1156, 1834
Bsp13I TCCGGA 1 cut(s) 799
Bsp1407I TGTACA 2 cut(s) 342, 699
BspACI CCGC 3 cut(s) 1787, 2257, 2287
BspANI GGCC 5 cut(s) 761, 1507, 1889, 2057, 2083
BspCNI CTCAG 6 cut(s) 120, 145, 429, 681, 1696, 1768
BspEI TCCGGA 1 cut(s) 799
BspFNI CGCG 1 cut(s) 1787
BspHI TCATGA 1 cut(s) 1396
BspLI GGNNCC 3 cut(s) 487, 591, 1030
BspMI ACCTGC 3 cut(s) 373, 724, 1123
BspPI GGATC 4 cut(s) 343, 781, 1227, 1442
BspQI GCTCTTC 1 cut(s) 1839
BspT104I TTCGAA 1 cut(s) 574
BsrDI GCAATG 3 cut(s) 1260, 2252, 2299
BsrFI RCCGGY 1 cut(s) 53
BsrGI TGTACA 2 cut(s) 342, 699
BsrI ACTGG 8 cut(s) 12, 763, 828, 1680, 1927, 2058, 2084, 2154
BssAI RCCGGY 1 cut(s) 53
BssECI CCNNGG 2 cut(s) 1069, 1508
BssT1I CCWWGG 1 cut(s) 1508
Bst2UI CCWGG 2 cut(s) 594, 791
Bst6I CTCTTC 2 cut(s) 1056, 1839
BstAPI GCANNNNNTGC 3 cut(s) 1943, 2042, 2243
BstAUI TGTACA 2 cut(s) 342, 699
BstBI TTCGAA 1 cut(s) 574
BstDEI CTNAG 7 cut(s) 107, 132, 437, 668, 1104, 1704, 1755
BstDSI CCRYGG 1 cut(s) 1069
BstENI CCTNNNNNAGG 1 cut(s) 788
BstF5I GGATG 6 cut(s) 415, 526, 808, 1363, 1848, 1984
BstFNI CGCG 1 cut(s) 1787
BstH2I RGCGCY 1 cut(s) 1516
BstHHI GCGC 1 cut(s) 1515
BstMAI GTCTC 4 cut(s) 105, 821, 1051, 1671
BstNI CCWGG 2 cut(s) 594, 791
BstNSI RCATGY 5 cut(s) 82, 1018, 1080, 1435, 1950
BstSCI CCNGG 3 cut(s) 592, 789, 1093
BstSFI CTRYAG 2 cut(s) 539, 1641
BstUI CGCG 1 cut(s) 1787
BstV1I GCAGC 4 cut(s) 904, 1696, 2029, 2146
BstV2I GAAGAC 3 cut(s) 27, 1119, 1619
BstX2I RGATCY 1 cut(s) 335
BstYI RGATCY 1 cut(s) 335
BsuI GTATCC 1 cut(s) 1864
BsuRI GGCC 5 cut(s) 761, 1507, 1889, 2057, 2083
BtgI CCRYGG 1 cut(s) 1069
BtgZI GCGATG 1 cut(s) 34
BtrI CACGTC 1 cut(s) 376
BtsCI GGATG 6 cut(s) 415, 526, 808, 1363, 1848, 1984
BtsI GCAGTG 3 cut(s) 1299, 1944, 2083
BveI ACCTGC 3 cut(s) 373, 724, 1123
CaiI CAGNNNCTG 2 cut(s) 823, 2231
CciI TCATGA 1 cut(s) 1396
CfoI GCGC 1 cut(s) 1515
Cfr10I RCCGGY 1 cut(s) 53
Cfr13I GGNCC 4 cut(s) 590, 760, 1505, 2081
CseI GACGC 3 cut(s) 104, 122, 1655
CsiI ACCWGGT 1 cut(s) 592
CviAII CATG 9 cut(s) 79, 251, 614, 1015, 1077, 1249, 1397, 1432, 1947
DdeI CTNAG 7 cut(s) 107, 132, 437, 668, 1104, 1704, 1755
DraI TTTAAA 1 cut(s) 2016
DraIII CACNNNGTG 1 cut(s) 1070
DrdI GACNNNNNNGTC 1 cut(s) 1567
DseDI GACNNNNNNGTC 1 cut(s) 1567
EaeI YGGCCR 1 cut(s) 2055
Eam1104I CTCTTC 2 cut(s) 1056, 1839
EarI CTCTTC 2 cut(s) 1056, 1839
Ecl136II GAGCTC 2 cut(s) 89, 148
Eco130I CCWWGG 1 cut(s) 1508
Eco24I GRGCYC 4 cut(s) 91, 150, 432, 1834
Eco32I GATATC 1 cut(s) 871
Eco47I GGWCC 1 cut(s) 590
Eco53kI GAGCTC 2 cut(s) 89, 148
Eco57I CTGAAG 2 cut(s) 839, 1820
Eco88I CYCGRG 3 cut(s) 157, 1209, 2067
EcoICRI GAGCTC 2 cut(s) 89, 148
EcoNI CCTNNNNNAGG 1 cut(s) 788
EcoO109I RGGNCCY 1 cut(s) 590
EcoRI GAATTC 1 cut(s) 1745
EcoRII CCWGG 2 cut(s) 592, 789
EcoRV GATATC 1 cut(s) 871
EcoT14I CCWWGG 1 cut(s) 1508
EcoT22I ATGCAT 3 cut(s) 84, 1948, 2239
EcoT38I GRGCYC 4 cut(s) 91, 150, 432, 1834
ErhI CCWWGG 1 cut(s) 1508
Esp3I CGTCTC 3 cut(s) 105, 1051, 1671
FaeI CATG 9 cut(s) 82, 254, 617, 1018, 1080, 1252, 1400, 1435, 1950
FalI AAGNNNNNCTT 6 cut(s) 363, 395, 1141, 1173, 2009, 2041
FaqI GGGAC 1 cut(s) 603
FatI CATG 9 cut(s) 78, 250, 613, 1014, 1076, 1248, 1396, 1431, 1946
FauI CCCGC 1 cut(s) 2280
FauNDI CATATG 1 cut(s) 1472
FbaI TGATCA 2 cut(s) 1344, 1972
FblI GTMKAC 1 cut(s) 1644
Fnu4HI GCNGC 4 cut(s) 918, 1710, 2043, 2160
FokI GGATG 6 cut(s) 402, 533, 815, 1370, 1855, 1991
FriOI GRGCYC 4 cut(s) 91, 150, 432, 1834
Fsp4HI GCNGC 4 cut(s) 918, 1710, 2043, 2160
FspBI CTAG 2 cut(s) 644, 2211
GlaI GCGC 1 cut(s) 1514
GluI GCNGC 4 cut(s) 918, 1710, 2043, 2160
GsaI CCCAGC 1 cut(s) 853
GsuI CTGGAG 2 cut(s) 242, 845
HaeII RGCGCY 1 cut(s) 1516
HaeIII GGCC 5 cut(s) 761, 1507, 1889, 2057, 2083
HapII CCGG 3 cut(s) 54, 800, 1094
HgaI GACGC 3 cut(s) 104, 122, 1655
HhaI GCGC 1 cut(s) 1515
Hin1II CATG 9 cut(s) 82, 254, 617, 1018, 1080, 1252, 1400, 1435, 1950
Hin6I GCGC 1 cut(s) 1513
HinP1I GCGC 1 cut(s) 1513
HincII GTYRAC 1 cut(s) 1012
HindII GTYRAC 1 cut(s) 1012
HindIII AAGCTT 3 cut(s) 563, 960, 1907
HinfI GANTC 5 cut(s) 15, 421, 1286, 1790, 2207
HpaII CCGG 3 cut(s) 54, 800, 1094
HphI GGTGA 2 cut(s) 1006, 1214
Hpy166II GTNNAC 4 cut(s) 343, 1012, 1645, 1952
Hpy8I GTNNAC 4 cut(s) 343, 1012, 1645, 1952
HpyAV CCTTC 3 cut(s) 38, 82, 491
HpyCH4IV ACGT 2 cut(s) 375, 1044
HpyF3I CTNAG 7 cut(s) 107, 132, 437, 668, 1104, 1704, 1755
HpySE526I ACGT 2 cut(s) 375, 1044
Hsp92II CATG 9 cut(s) 82, 254, 617, 1018, 1080, 1252, 1400, 1435, 1950
HspAI GCGC 1 cut(s) 1513
Kpn2I TCCGGA 1 cut(s) 799
KroI GCCGGC 1 cut(s) 53
KroNI GCCGGC 1 cut(s) 55
Ksp22I TGATCA 2 cut(s) 1344, 1972
LguI GCTCTTC 1 cut(s) 1839
LmnI GCTCC 4 cut(s) 153, 1028, 1073, 1159
Lsp1109I GCAGC 4 cut(s) 904, 1696, 2029, 2146
LweI GCATC 5 cut(s) 57, 91, 126, 511, 2224
MabI ACCWGGT 1 cut(s) 592
MaeI CTAG 2 cut(s) 644, 2211
MaeII ACGT 2 cut(s) 375, 1044
MaeIII GTNAC 8 cut(s) 443, 471, 514, 1180, 1804, 2062, 2086, 2204
MflI RGATCY 1 cut(s) 335
MhlI GDGCHC 5 cut(s) 91, 150, 432, 1156, 1834
MlsI TGGCCA 1 cut(s) 2057
MluNI TGGCCA 1 cut(s) 2057
MlyI GAGTC 2 cut(s) 1280, 2201
MmeI TCCRAC 2 cut(s) 1006, 1567
Mox20I TGGCCA 1 cut(s) 2057
Mph1103I ATGCAT 3 cut(s) 84, 1948, 2239
MroI TCCGGA 1 cut(s) 799
MroNI GCCGGC 1 cut(s) 53
MroXI GAANNNNTTC 1 cut(s) 948
MscI TGGCCA 1 cut(s) 2057
MslI CAYNNNNRTG 1 cut(s) 1253
Msp20I TGGCCA 1 cut(s) 2057
MspI CCGG 3 cut(s) 54, 800, 1094
MspR9I CCNGG 3 cut(s) 594, 791, 1095
Mva1269I GAATGC 2 cut(s) 57, 582
MvaI CCWGG 2 cut(s) 594, 791
MvnI CGCG 1 cut(s) 1787
NaeI GCCGGC 1 cut(s) 55
NciI CCSGG 1 cut(s) 1095
NdeI CATATG 1 cut(s) 1472
NgoMIV GCCGGC 1 cut(s) 53
NlaIII CATG 9 cut(s) 82, 254, 617, 1018, 1080, 1252, 1400, 1435, 1950
NlaIV GGNNCC 3 cut(s) 487, 591, 1030
NmeAIII GCCGAG 1 cut(s) 107
NmuCI GTSAC 4 cut(s) 1180, 1804, 2086, 2204
NsiI ATGCAT 3 cut(s) 84, 1948, 2239
NspI RCATGY 5 cut(s) 82, 1018, 1080, 1435, 1950
NspV TTCGAA 1 cut(s) 574
PaeR7I CTCGAG 2 cut(s) 157, 1209
PagI TCATGA 1 cut(s) 1396
PaqCI CACCTGC 1 cut(s) 373
PciSI GCTCTTC 1 cut(s) 1839
PctI GAATGC 2 cut(s) 57, 582
PdiI GCCGGC 1 cut(s) 55
PdmI GAANNNNTTC 1 cut(s) 948
PfeI GAWTC 3 cut(s) 15, 421, 1790
PflFI GACNNNGTC 2 cut(s) 594, 1178
PkrI GCNGC 4 cut(s) 919, 1711, 2044, 2161
PleI GAGTC 2 cut(s) 1280, 2201
PpsI GAGTC 2 cut(s) 1280, 2201
PpuMI RGGWCCY 1 cut(s) 590
PshBI ATTAAT 2 cut(s) 893, 1331
PsiI TTATAA 1 cut(s) 1440
Psp124BI GAGCTC 2 cut(s) 91, 150
Psp5II RGGWCCY 1 cut(s) 590
Psp6I CCWGG 2 cut(s) 592, 789
PspFI CCCAGC 1 cut(s) 849
PspGI CCWGG 2 cut(s) 592, 789
PspN4I GGNNCC 3 cut(s) 487, 591, 1030
PspPI GGNCC 4 cut(s) 590, 760, 1505, 2081
PspPPI RGGWCCY 1 cut(s) 590
PspXI VCTCGAGB 2 cut(s) 157, 1209
PsrI GAACNNNNNNTAC 2 cut(s) 2252, 2284
PstNI CAGNNNCTG 2 cut(s) 823, 2231
PsuI RGATCY 1 cut(s) 335
PsyI GACNNNGTC 2 cut(s) 594, 1178
RseI CAYNNNNRTG 1 cut(s) 1253
SacI GAGCTC 2 cut(s) 91, 150
SapI GCTCTTC 1 cut(s) 1839
SatI GCNGC 4 cut(s) 918, 1710, 2043, 2160
Sau96I GGNCC 4 cut(s) 590, 760, 1505, 2081
SchI GAGTC 2 cut(s) 1280, 2201
ScrFI CCNGG 3 cut(s) 594, 791, 1095
SduI GDGCHC 5 cut(s) 91, 150, 432, 1156, 1834
SexAI ACCWGGT 1 cut(s) 592
SfaNI GCATC 5 cut(s) 57, 91, 126, 511, 2224
SfcI CTRYAG 2 cut(s) 539, 1641
Sfr274I CTCGAG 2 cut(s) 157, 1209
SfuI TTCGAA 1 cut(s) 574
SinI GGWCC 1 cut(s) 590
SlaI CTCGAG 2 cut(s) 157, 1209
SmiMI CAYNNNNRTG 1 cut(s) 1253
SmlI CTYRAG 5 cut(s) 157, 453, 1209, 1800, 2026
SmoI CTYRAG 5 cut(s) 157, 453, 1209, 1800, 2026
SsiI CCGC 3 cut(s) 1787, 2257, 2287
SspMI CTAG 2 cut(s) 644, 2211
SstI GAGCTC 2 cut(s) 91, 150
StyD4I CCNGG 3 cut(s) 592, 789, 1093
StyI CCWWGG 1 cut(s) 1508
TaiI ACGT 2 cut(s) 378, 1047
TaqI TCGA 8 cut(s) 18, 85, 158, 204, 260, 574, 957, 1210
TatI WGTACW 5 cut(s) 342, 699, 1167, 1689, 1765
TfiI GAWTC 3 cut(s) 15, 421, 1790
TseFI GTSAC 4 cut(s) 1180, 1804, 2086, 2204
TseI GCWGC 4 cut(s) 917, 1709, 2042, 2159
Tsp45I GTSAC 4 cut(s) 1180, 1804, 2086, 2204
TspDTI ATGAA 9 cut(s) 510, 572, 630, 1292, 1385, 1511, 1758, 1788, 1859
TspGWI ACGGA 4 cut(s) 27, 533, 1058, 1653
Tth111I GACNNNGTC 2 cut(s) 594, 1178
VpaK11BI GGWCC 1 cut(s) 590
VspI ATTAAT 2 cut(s) 893, 1331
XagI CCTNNNNNAGG 1 cut(s) 788
XapI RAATTY 4 cut(s) 544, 944, 1408, 1745
XceI RCATGY 5 cut(s) 82, 1018, 1080, 1435, 1950
XhoI CTCGAG 2 cut(s) 157, 1209
XmiI GTMKAC 1 cut(s) 1644
XmnI GAANNNNTTC 1 cut(s) 948
XspI CTAG 2 cut(s) 644, 2211
Zsp2I ATGCAT 3 cut(s) 84, 1948, 2239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.