Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002973

Physical Location & Seq

Genomic Coordinates Forward (+)

1850516 .. 1856022

Gene Structure 5507 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1030100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2775 bp

ATGTATGCGGTTGGGAGGCTAGGGAGTTATATATCCAGAGGGGTATACACCGTCTCCGGCCCGTTCCACCCTTTTGGTGGAGCTGTGGACATAATTGTAGTTGAACAACAAGATGGGAGCTTCAAATCTTCAGCGTGGAATGTGAAATTTGGGAAGTTTCAAGGGGTTTTGAAGACAAAGGAGAAGGTGGTTAACATTAATGTCAATGGGGAGGATGCTAGTTTCCACATGTATTTGGATCATAAAGGGGAAGCTTATTTTCTTAGGGAGGTTGATGCAGAAGAAGGGGAGGCTGTGTTGTATCCTTCGTCTTCGAGTGATGAGACGGATGAGCAATGTCGGGAAAACAGGCAGCCATTGAAGTCCATGAGTTGCAATTATGATGCTGAGAATTTGACAACAGTTGATCAGATTCAAACGCTTAACGGGAAGATTTTGTCCAGGACTAATTCCCGGAAGTCACAAATTTTTGGGCTATTTGGGAGCAGGTCGACGAAGGAGCGCAAGAGTAAGGAGGAAGAGGGGGTTGATAGTGAGGTGAGAAGGGTGGATTCATTGGAGCGGGCCGAGTTTGCGGCTAACCTTTTGGAGGTGAAGTGGTCCACTAGTCTTGCCACTAAGAAATTCGGGAAGGATAATGCTTCCCGTTTTTCGTCTCCTAATGTGTTAGTTAATGAAGATATGCAGATTGATGCCGAGGAAAGCCGGCTGAGCTCATCTCTGCCTGTGTCTAGAGTATCTAGTGATGTTGGTGAATCTTTTATTGCAGCAACCGAACACGATGGGAATGCAATTGCTGTCATATCCAATGTCACAGGGTCTGATCTTGAAATTCAAAGTTCTTTTGAGCTAGAAGCTTGCCCTGGCAAGCATTTGGATGAGAAAGATGTTGTATTACCTGGCCGTGACATTTCTCATACGGCGGGTGGGATAGATGGAGTCCAACCCTTTATTTATTGTGAAACATTGTACATTGCCAGAGAAAGAAGTGGGCAAGTTCATGTTCATGCCAGAACTGAGCTGCTATCTAAGGATTCAGTTACTGAGCAACATGCTGATGATACTTCCATGGAGGTACATTCAGTGGAGGCTACTGAAACTTGTGCACAAGAGACAGATGCACATTCATGCACAGACCGTAATATTGACCTTGAAGGGCCAGCAATAGTTCAACAATACAATATTCAGACAGTCCAGCGAAATTCATTACCTGGTTCAGTTAAAAAAGTTGAGTCACAGAGCATGTTTAGTTTAAACTACTCTGGTGATCAAGTTCAAGAAGAGAAAAATACAAAAGAGGAAGAAATCGGATCATGCCATTTACAAACCCCGTCTGAGTCCGTTATTGGTCATGTCATGACAGAAGCAGCAAGTGTGTTGCCAGTGGGATTAGAGGAAGAACAATTTCTCTTCAGTGACGATGAAATCAGAATCACCGAGGTTCAGTGCACGGAATCGTGTTCTTCATCATGTGTAGACAGAGAAAATAGTCTCTCGTGTAGTCCAAAAGACAGTACAAACTATGAATCATATTCATCTCCAGAAAAGTTTGTTCCAGAAGACCCCTCAATTGATTTTGAGAAATCAAATAGAAAACTGGAGGCAGCATCAGCTGCAATTGGTATTCCTAGAAATCGTAAGGCTGCTGTTGATAAAGAAGTCAGGAAGCTGGTCACGTCATTACCCAACATGTGGCCTCATACTGACAAGTTGAGCGCACTTGATCTTCATTATCCCTTGAGCTGTTCACTGGACTCAAATGTCAATCCCTTGAAGTCAATATGGCAAGGTAAAGATGATTTGAGCTGTAGAAAGTGGGACACAGAAGAAGAGAAAAAATTAGCACTGGAAACACCAAATATTGAGAATACTCAGGGTTCAGTGGAGCTCAAAGGTGGTCCTGCCATTCCTGCAGTTGGAGATCCATCTAAAGTAAATGTTGCCGCCAGTGGAAGTTGGAAACTCTGGCCTTTCGGTTCAAGGAGATCCAATTCCAGGAAAGCAATGCAGCCAGATGTGAATGGTGGTAAAAGTCCTGATGCTCAGAATGCTACAGAGAGCACCTTTGCCACCGATGGAGAGAAGAATATGCTTACAGATGGAGATATGAATATGCTTACAGATGGAGATAAGAATATGCTTACAGATGGGGATAAGAATATGCTTGCACCGAAGGGGATGAAGAAAATGGAGAGGGTACTCGCTCCAACATCTGAGCAGCTGGCGTCCTTAAAACTCAAAGAAGGGAAGAATACAGTAACCTTCACATTCTTTACTGCAATGCTGGGGAAGCAGCAGGTTGATGCCAGAATTTATCTGTGGAAATGGAACACTCGCATAGTGATCTCAGACGTGGATGGGACAATCACAAAGTCAGATGTTCTAGGTCAATTCATGCCCTTGGTTGGGGTAGATTGGTCACATATAGGTGTTACAAATTTGTTTTCTGCCATTAAGGAAAATGGATATCAATTGCTTTTTTTGAGTGCACGTGCCATTTCTCAAGCCTCTGTCACCAGACAATTTTTATTCAACCTTAAGCAGGATGGGAAGGCTTTACCAGATGGTCCCGTTGTTATTTCCCCAGATGGACTTTTTCCTTCTCTCTTTCGCGAAGTTATCAGGAGGGCTCCTCATGAATTCAAAATCGCATGCTTGGAGGATATCAAATCATTGTTCCCTTCTGATTGCAATCCATTCTATGCTGGTTTTGGAAACAGAGATACCGATGAGTTCAGCTACCTTAAGGTTGGAATCCCGACAAGGGAAAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006629 GO:0006643 GO:0006644 GO:0006664 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009058 GO:0009247 GO:0009987 GO:0016020 GO:0019374 GO:0019375 GO:0019637 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0046467 GO:0048046 GO:0071704 GO:0090407 GO:1901135 GO:1901137 GO:1901576 GO:1903509

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 map00561 map00564 map01100 map01110

Pfam Domains

Protein Families

LNS2 Lipin_N Lipin_mid

Protein Analysis

925

Amino Acids

102.02

Weight (kDa)

5.03

Isoelectric Point (pI)

44.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lipin_N	PF04571	12 - 97	4.2e-27	lipin, N-terminal conserved region
LNS2	PF08235	733 - 921	1.8e-83	LNS2 (Lipin/Ned1/Smp2)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0002973)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G42870 AT5G42870
fragaria_vesca	FvH4_2g34540 FvH4_2g34540 FvH4_2g34540 FvH4_2g34540 FvH4_2g34540 FvH4_2g34540 FvH4_2g34540 FvH4_2g34540 FvH4_2g34540 FvH4_2g34540 FvH4_2g34540 FvH4_2g34540 FvH4_2g34540 FvH4_2g34540 FvH4_2g34540 FvH4_2g34540 FvH4_2g34540 FvH4_2g34540
malus_domestica	MD15G1030100.v1.1 MD15G1084000.v1.1
prunus_persica	Prupe.1G437400_v2.0.a1 Prupe.1G437400_v2.0.a1 Prupe.1G437400_v2.0.a1
pyrus_communis	pycom08g08400 pycom15g07920
rosa_chinensis	RchiOBHm_Chr6g0307611 RchiOBHm_Chr6g0307781
rosa_laevigata	RLG00000010701
rosa_multiflora	Rmu_sc0000031.1_g000031
rosa_roxburghii	Rroxscaffold_7G00160890
rosa_rugosa	Rorug06G0360900
rosa_samantha	Rh6AG469900 Rh6BG480400 Rh6CG486100 Rh6DG472500
rosa_wichuraiana	Rw6G041030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1760
Acc36I	ACCTGC	2 cut(s)	477, 2287
AccB7I	CCANNNNNTGG	1 cut(s)	1692
AccBSI	CCGCTC	1 cut(s)	562
AccI	GTMKAC	3 cut(s)	45, 491, 1476
AccII	CGCG	1 cut(s)	2613
AciI	CCGC	5 cut(s)	8, 562, 575, 923, 1944
AclWI	GGATC	4 cut(s)	246, 1318, 1916, 1980
AcoI	YGGCCR	1 cut(s)	901
AcsI	RAATTY	9 cut(s)	146, 391, 465, 623, 831, 1201, 2310, 2437, 2639
AcuI	CTGAAG	2 cut(s)	114, 1396
AcvI	CACGTG	1 cut(s)	2492
AcyI	GRCGYC	1 cut(s)	2225
AfaI	GTAC	4 cut(s)	971, 1077, 1516, 2199
AfiI	CCNNNNNNNGG	9 cut(s)	77, 589, 1692, 1916, 1995, 2405, 2406, 2542, 2764
AflII	CTTAAG	2 cut(s)	2537, 2744
AflIII	ACRYGT	2 cut(s)	228, 1689
AhlI	ACTAGT	1 cut(s)	605
AjiI	CACGTC	2 cut(s)	1677, 2353
AjnI	CCWGG	5 cut(s)	440, 862, 898, 1210, 1994
Alw21I	GWGCWC	6 cut(s)	716, 1108, 1451, 1890, 2063, 2491
Alw26I	GTCTC	5 cut(s)	58, 317, 660, 1106, 1496
Alw44I	GTGCAC	3 cut(s)	1104, 1447, 2487
AlwI	GGATC	4 cut(s)	246, 1318, 1916, 1980
AlwNI	CAGNNNCTG	2 cut(s)	821, 1043
AoxI	GGCC	6 cut(s)	58, 564, 901, 1157, 1694, 1967
ApaLI	GTGCAC	3 cut(s)	1104, 1447, 2487
ApoI	RAATTY	9 cut(s)	146, 391, 465, 623, 831, 1201, 2310, 2437, 2639
AseI	ATTAAT	1 cut(s)	198
Asp700I	GAANNNNTTC	1 cut(s)	1404
AspLEI	GCGC	2 cut(s)	504, 1718
AspS9I	GGNCC	6 cut(s)	59, 564, 600, 1157, 1898, 2567
AsuC2I	CCSGG	1 cut(s)	454
AsuHPI	GGTGA	6 cut(s)	550, 604, 764, 1277, 1426, 2506
AvaII	GGWCC	3 cut(s)	600, 1898, 2567
BaeGI	GKGCMC	3 cut(s)	1108, 1451, 2491
BanII	GRGCYC	3 cut(s)	716, 1890, 2632
BauI	CACGAG	1 cut(s)	1495
BbrPI	CACGTG	1 cut(s)	2492
BbsI	GAAGAC	3 cut(s)	179, 303, 1566
Bbv12I	GWGCWC	6 cut(s)	716, 1108, 1451, 1890, 2063, 2491
BceAI	ACGGC	2 cut(s)	888, 936
BcgI	CGANNNNNNTGC	2 cut(s)	770, 804
BciT130I	CCWGG	5 cut(s)	442, 864, 900, 1212, 1996
BciVI	GTATCC	1 cut(s)	312
BclI	TGATCA	2 cut(s)	406, 1267
BcnI	CCSGG	1 cut(s)	454
BcoDI	GTCTC	5 cut(s)	58, 317, 660, 1106, 1496
BcuI	ACTAGT	1 cut(s)	605
BfaI	CTAG	8 cut(s)	20, 219, 606, 732, 741, 851, 1629, 2384
BfmI	CTRYAG	3 cut(s)	1807, 1911, 2052
BfrI	CTTAAG	2 cut(s)	2537, 2744
BfuAI	ACCTGC	2 cut(s)	477, 2287
BfuI	GTATCC	1 cut(s)	312
BlpI	GCTNAGC	1 cut(s)	710
Bme1390I	CCNGG	6 cut(s)	442, 454, 864, 900, 1212, 1996
Bme18I	GGWCC	3 cut(s)	600, 1898, 2567
BmgBI	CACGTC	2 cut(s)	1677, 2353
BmgT120I	GGNCC	6 cut(s)	59, 564, 600, 1157, 1898, 2567
BmiI	GGNNCC	2 cut(s)	2569, 2631
BmrFI	CCNGG	6 cut(s)	442, 454, 864, 900, 1212, 1996
BmsI	GCATC	8 cut(s)	205, 265, 373, 682, 1108, 1616, 2029, 2293
BpiI	GAAGAC	3 cut(s)	179, 303, 1566
BplI	GAGNNNNNCTC	4 cut(s)	703, 735, 2617, 2649
BpmI	CTGGAG	2 cut(s)	1524, 1619
Bpu1102I	GCTNAGC	1 cut(s)	710
BpuEI	CTTGAG	2 cut(s)	1759, 2487
BpuMI	CCSGG	1 cut(s)	454
BsaAI	YACGTR	1 cut(s)	2492
BsaBI	GATNNNNATC	1 cut(s)	2691
BsaHI	GRCGYC	1 cut(s)	2225
BsaJI	CCNNGG	5 cut(s)	696, 862, 1068, 1437, 2400
BsaXI	ACNNNNNCTCC	6 cut(s)	38, 68, 281, 311, 581, 611
Bsc4I	CCNNNNNNNGG	9 cut(s)	77, 589, 1692, 1916, 1995, 2405, 2406, 2542, 2764
Bse118I	RCCGGY	1 cut(s)	705
Bse1I	ACTGG	5 cut(s)	1382, 1602, 1755, 1851, 1947
Bse3DI	GCAATG	4 cut(s)	341, 972, 2010, 2286
Bse8I	GATNNNNATC	1 cut(s)	2691
BseBI	CCWGG	5 cut(s)	442, 864, 900, 1212, 1996
BseDI	CCNNGG	5 cut(s)	696, 862, 1068, 1437, 2400
BseGI	GGATG	6 cut(s)	220, 334, 883, 2184, 2362, 2551
BseJI	GATNNNNATC	1 cut(s)	2691
BseLI	CCNNNNNNNGG	9 cut(s)	77, 589, 1692, 1916, 1995, 2405, 2406, 2542, 2764
BseMI	GCAATG	4 cut(s)	341, 972, 2010, 2286
BseMII	CTCAG	9 cut(s)	378, 701, 1008, 1035, 1326, 1886, 2057, 2205, 2361
BseNI	ACTGG	5 cut(s)	1382, 1602, 1755, 1851, 1947
BseRI	GAGGAG	1 cut(s)	2622
BseSI	GKGCMC	3 cut(s)	1108, 1451, 2491
BseYI	CCCAGC	1 cut(s)	2284
Bsh1236I	CGCG	1 cut(s)	2613
BshFI	GGCC	6 cut(s)	60, 566, 903, 1159, 1696, 1969
BsiHKAI	GWGCWC	6 cut(s)	716, 1108, 1451, 1890, 2063, 2491
BsiSI	CCGG	3 cut(s)	57, 454, 706
BslFI	GGGAC	3 cut(s)	1832, 2374, 2553
BslI	CCNNNNNNNGG	9 cut(s)	77, 589, 1692, 1916, 1995, 2405, 2406, 2542, 2764
BsmAI	GTCTC	5 cut(s)	58, 317, 660, 1106, 1496
BsmBI	CGTCTC	3 cut(s)	58, 317, 660
BsmFI	GGGAC	3 cut(s)	1832, 2374, 2553
BsmI	GAATGC	2 cut(s)	793, 2053
BsnI	GGCC	6 cut(s)	60, 566, 903, 1159, 1696, 1969
Bsp1286I	GDGCHC	7 cut(s)	716, 1108, 1451, 1890, 2063, 2491, 2632
Bsp1407I	TGTACA	1 cut(s)	969
Bsp143I	GATC	9 cut(s)	238, 406, 823, 1267, 1310, 1723, 1921, 1985, 2343
Bsp1720I	GCTNAGC	1 cut(s)	710
Bsp19I	CCATGG	1 cut(s)	1068
Bsp68I	TCGCGA	1 cut(s)	2613
BspACI	CCGC	5 cut(s)	8, 562, 575, 923, 1944
BspANI	GGCC	6 cut(s)	60, 566, 903, 1159, 1696, 1969
BspCNI	CTCAG	9 cut(s)	379, 702, 1009, 1036, 1327, 1885, 2056, 2206, 2360
BspFNI	CGCG	1 cut(s)	2613
BspHI	TCATGA	2 cut(s)	1356, 2635
BspLI	GGNNCC	2 cut(s)	2569, 2631
BspMAI	CTGCAG	1 cut(s)	1915
BspMI	ACCTGC	2 cut(s)	477, 2287
BspPI	GGATC	4 cut(s)	246, 1318, 1916, 1980
BspTI	CTTAAG	2 cut(s)	2537, 2744
BsrBI	CCGCTC	1 cut(s)	562
BsrDI	GCAATG	4 cut(s)	341, 972, 2010, 2286
BsrFI	RCCGGY	1 cut(s)	705
BsrGI	TGTACA	1 cut(s)	969
BsrI	ACTGG	5 cut(s)	1382, 1602, 1755, 1851, 1947
BssAI	RCCGGY	1 cut(s)	705
BssECI	CCNNGG	5 cut(s)	696, 862, 1068, 1437, 2400
BssMI	GATC	9 cut(s)	238, 406, 823, 1267, 1310, 1723, 1921, 1985, 2343
BssNAI	GTATAC	1 cut(s)	46
BssNI	GRCGYC	1 cut(s)	2225
BssSI	CACGAG	1 cut(s)	1495
BssT1I	CCWWGG	2 cut(s)	1068, 2400
Bst1107I	GTATAC	1 cut(s)	46
Bst2BI	CACGAG	1 cut(s)	1495
Bst2UI	CCWGG	5 cut(s)	442, 864, 900, 1212, 1996
Bst4CI	ACNGT	6 cut(s)	52, 403, 1139, 1192, 1514, 2257
Bst6I	CTCTTC	4 cut(s)	513, 1275, 1415, 1824
BstACI	GRCGYC	1 cut(s)	2225
BstAFI	CTTAAG	2 cut(s)	2537, 2744
BstAPI	GCANNNNNTGC	1 cut(s)	1613
BstAUI	TGTACA	1 cut(s)	969
BstBAI	YACGTR	1 cut(s)	2492
BstC8I	GCNNGC	8 cut(s)	564, 707, 859, 869, 1161, 2166, 2223, 2653
BstDSI	CCRYGG	1 cut(s)	1068
BstENI	CCTNNNNNAGG	2 cut(s)	587, 2540
BstF5I	GGATG	6 cut(s)	220, 334, 883, 2184, 2362, 2551
BstFNI	CGCG	1 cut(s)	2613
BstHHI	GCGC	2 cut(s)	504, 1718
BstKTI	GATC	9 cut(s)	241, 409, 826, 1270, 1313, 1726, 1924, 1988, 2346
BstMAI	GTCTC	5 cut(s)	58, 317, 660, 1106, 1496
BstMBI	GATC	9 cut(s)	238, 406, 823, 1267, 1310, 1723, 1921, 1985, 2343
BstMWI	GCNNNNNNNGC	7 cut(s)	572, 711, 1610, 1613, 1910, 2048, 2290
BstNI	CCWGG	5 cut(s)	442, 864, 900, 1212, 1996
BstNSI	RCATGY	5 cut(s)	232, 1055, 1246, 1693, 2655
BstSCI	CCNGG	6 cut(s)	440, 452, 862, 898, 1210, 1994
BstSFI	CTRYAG	3 cut(s)	1807, 1911, 2052
BstSLI	GKGCMC	3 cut(s)	1108, 1451, 2491
BstUI	CGCG	1 cut(s)	2613
BstV2I	GAAGAC	3 cut(s)	179, 303, 1566
BstX2I	RGATCY	2 cut(s)	1921, 1985
BstXI	CCANNNNNNTGG	1 cut(s)	74
BstYI	RGATCY	2 cut(s)	1921, 1985
BstZ17I	GTATAC	1 cut(s)	46
BsuI	GTATCC	1 cut(s)	312
BsuRI	GGCC	6 cut(s)	60, 566, 903, 1159, 1696, 1969
BtgI	CCRYGG	1 cut(s)	1068
BtrI	CACGTC	2 cut(s)	1677, 2353
BtsCI	GGATG	6 cut(s)	220, 334, 883, 2184, 2362, 2551
BtsIMutI	CAGTG	8 cut(s)	1089, 1389, 1420, 1451, 1748, 1844, 1887, 1954
BtuMI	TCGCGA	1 cut(s)	2613
BveI	ACCTGC	2 cut(s)	477, 2287
Cac8I	GCNNGC	8 cut(s)	564, 707, 859, 869, 1161, 2166, 2223, 2653
CaiI	CAGNNNCTG	2 cut(s)	821, 1043
CciI	TCATGA	2 cut(s)	1356, 2635
CfoI	GCGC	2 cut(s)	504, 1718
Cfr10I	RCCGGY	1 cut(s)	705
Cfr13I	GGNCC	6 cut(s)	59, 564, 600, 1157, 1898, 2567
CseI	GACGC	1 cut(s)	2214
CsiI	ACCWGGT	1 cut(s)	1210
Csp6I	GTAC	4 cut(s)	970, 1076, 1515, 2198
CspCI	CAANNNNNGTGG	2 cut(s)	592, 627
CviQI	GTAC	4 cut(s)	970, 1076, 1515, 2198
DpnI	GATC	9 cut(s)	240, 408, 825, 1269, 1312, 1725, 1923, 1987, 2345
DpnII	GATC	9 cut(s)	238, 406, 823, 1267, 1310, 1723, 1921, 1985, 2343
DraI	TTTAAA	1 cut(s)	1254
DrdI	GACNNNNNNGTC	1 cut(s)	1760
DseDI	GACNNNNNNGTC	1 cut(s)	1760
EaeI	YGGCCR	1 cut(s)	901
Eam1104I	CTCTTC	4 cut(s)	513, 1275, 1415, 1824
EarI	CTCTTC	4 cut(s)	513, 1275, 1415, 1824
Ecl136II	GAGCTC	2 cut(s)	714, 1888
Eco130I	CCWWGG	2 cut(s)	1068, 2400
Eco24I	GRGCYC	3 cut(s)	716, 1890, 2632
Eco32I	GATATC	2 cut(s)	2468, 2665
Eco47I	GGWCC	3 cut(s)	600, 1898, 2567
Eco53kI	GAGCTC	2 cut(s)	714, 1888
Eco57I	CTGAAG	2 cut(s)	114, 1396
Eco72I	CACGTG	1 cut(s)	2492
EcoICRI	GAGCTC	2 cut(s)	714, 1888
EcoNI	CCTNNNNNAGG	2 cut(s)	587, 2540
EcoRI	GAATTC	1 cut(s)	2639
EcoRII	CCWGG	5 cut(s)	440, 862, 898, 1210, 1994
EcoRV	GATATC	2 cut(s)	2468, 2665
EcoT14I	CCWWGG	2 cut(s)	1068, 2400
EcoT38I	GRGCYC	3 cut(s)	716, 1890, 2632
ErhI	CCWWGG	2 cut(s)	1068, 2400
Esp3I	CGTCTC	3 cut(s)	58, 317, 660
FaqI	GGGAC	3 cut(s)	1832, 2374, 2553
FauI	CCCGC	2 cut(s)	555, 916
FbaI	TGATCA	2 cut(s)	406, 1267
FblI	GTMKAC	3 cut(s)	45, 491, 1476
FokI	GGATG	6 cut(s)	227, 341, 890, 2191, 2369, 2558
FriOI	GRGCYC	3 cut(s)	716, 1890, 2632
FspBI	CTAG	8 cut(s)	20, 219, 606, 732, 741, 851, 1629, 2384
GlaI	GCGC	2 cut(s)	503, 1717
GsaI	CCCAGC	1 cut(s)	2288
GsuI	CTGGAG	2 cut(s)	1524, 1619
HaeIII	GGCC	6 cut(s)	60, 566, 903, 1159, 1696, 1969
HapII	CCGG	3 cut(s)	57, 454, 706
HgaI	GACGC	1 cut(s)	2214
HhaI	GCGC	2 cut(s)	504, 1718
Hin1I	GRCGYC	1 cut(s)	2225
Hin6I	GCGC	2 cut(s)	502, 1716
HinP1I	GCGC	2 cut(s)	502, 1716
HincII	GTYRAC	2 cut(s)	193, 492
HindII	GTYRAC	2 cut(s)	193, 492
HindIII	AAGCTT	2 cut(s)	252, 855
HpaI	GTTAAC	1 cut(s)	193
HpaII	CCGG	3 cut(s)	57, 454, 706
HphI	GGTGA	6 cut(s)	550, 604, 764, 1277, 1426, 2506
Hpy99I	CGWCG	1 cut(s)	496
HpyCH4III	ACNGT	6 cut(s)	52, 403, 1139, 1192, 1514, 2257
HpyCH4IV	ACGT	3 cut(s)	1676, 2352, 2491
HpyF10VI	GCNNNNNNNGC	7 cut(s)	572, 711, 1610, 1613, 1910, 2048, 2290
HpySE526I	ACGT	3 cut(s)	1676, 2352, 2491
Hsp92I	GRCGYC	1 cut(s)	2225
HspAI	GCGC	2 cut(s)	502, 1716
KroI	GCCGGC	1 cut(s)	705
KroNI	GCCGGC	1 cut(s)	707
Ksp22I	TGATCA	2 cut(s)	406, 1267
KspAI	GTTAAC	1 cut(s)	193
Kzo9I	GATC	9 cut(s)	238, 406, 823, 1267, 1310, 1723, 1921, 1985, 2343
LmnI	GCTCC	8 cut(s)	80, 117, 483, 499, 559, 1885, 2209, 2635
LweI	GCATC	8 cut(s)	205, 265, 373, 682, 1108, 1616, 2029, 2293
MabI	ACCWGGT	1 cut(s)	1210
MaeI	CTAG	8 cut(s)	20, 219, 606, 732, 741, 851, 1629, 2384
MaeII	ACGT	3 cut(s)	1676, 2352, 2491
MalI	GATC	9 cut(s)	240, 408, 825, 1269, 1312, 1725, 1923, 1987, 2345
MbiI	CCGCTC	1 cut(s)	562
MboI	GATC	9 cut(s)	238, 406, 823, 1267, 1310, 1723, 1921, 1985, 2343
MfeI	CAATTG	4 cut(s)	792, 1569, 1617, 2471
MflI	RGATCY	2 cut(s)	1921, 1985
MhlI	GDGCHC	7 cut(s)	716, 1108, 1451, 1890, 2063, 2491, 2632
MlyI	GAGTC	4 cut(s)	948, 1241, 1346, 1748
MmeI	TCCRAC	5 cut(s)	967, 1897, 1937, 2231, 2731
MroNI	GCCGGC	1 cut(s)	705
MroXI	GAANNNNTTC	1 cut(s)	1404
MslI	CAYNNNNRTG	5 cut(s)	876, 1005, 1056, 1126, 2427
MspA1I	CMGCKG	2 cut(s)	1613, 2221
MspCI	CTTAAG	2 cut(s)	2537, 2744
MspI	CCGG	3 cut(s)	57, 454, 706
MspR9I	CCNGG	6 cut(s)	442, 454, 864, 900, 1212, 1996
MssI	GTTTAAAC	1 cut(s)	1254
MunI	CAATTG	4 cut(s)	792, 1569, 1617, 2471
Mva1269I	GAATGC	2 cut(s)	793, 2053
MvaI	CCWGG	5 cut(s)	442, 864, 900, 1212, 1996
MvnI	CGCG	1 cut(s)	2613
MwoI	GCNNNNNNNGC	7 cut(s)	572, 711, 1610, 1613, 1910, 2048, 2290
NaeI	GCCGGC	1 cut(s)	707
NciI	CCSGG	1 cut(s)	454
NcoI	CCATGG	1 cut(s)	1068
NdeII	GATC	9 cut(s)	238, 406, 823, 1267, 1310, 1723, 1921, 1985, 2343
NgoMIV	GCCGGC	1 cut(s)	705
NlaIV	GGNNCC	2 cut(s)	2569, 2631
NmeAIII	GCCGAG	2 cut(s)	592, 721
NmuCI	GTSAC	8 cut(s)	459, 811, 905, 1233, 1415, 1672, 2418, 2512
NruI	TCGCGA	1 cut(s)	2613
NspI	RCATGY	5 cut(s)	232, 1055, 1246, 1693, 2655
PaeI	GCATGC	1 cut(s)	2655
PagI	TCATGA	2 cut(s)	1356, 2635
PciI	ACATGT	2 cut(s)	228, 1689
PctI	GAATGC	2 cut(s)	793, 2053
PdiI	GCCGGC	1 cut(s)	707
PdmI	GAANNNNTTC	1 cut(s)	1404
PfeI	GAWTC	8 cut(s)	412, 551, 755, 1034, 1431, 1454, 1526, 2754
PflMI	CCANNNNNTGG	1 cut(s)	1692
PfoI	TCCNGGA	3 cut(s)	440, 452, 1994
PleI	GAGTC	4 cut(s)	947, 1240, 1345, 1748
PmaCI	CACGTG	1 cut(s)	2492
PmeI	GTTTAAAC	1 cut(s)	1254
PmlI	CACGTG	1 cut(s)	2492
PpsI	GAGTC	4 cut(s)	947, 1240, 1345, 1748
Ppu21I	YACGTR	1 cut(s)	2492
PscI	ACATGT	2 cut(s)	228, 1689
PshBI	ATTAAT	1 cut(s)	198
Psp124BI	GAGCTC	2 cut(s)	716, 1890
Psp6I	CCWGG	5 cut(s)	440, 862, 898, 1210, 1994
PspCI	CACGTG	1 cut(s)	2492
PspFI	CCCAGC	1 cut(s)	2284
PspGI	CCWGG	5 cut(s)	440, 862, 898, 1210, 1994
PspN4I	GGNNCC	2 cut(s)	2569, 2631
PspPI	GGNCC	6 cut(s)	59, 564, 600, 1157, 1898, 2567
PstI	CTGCAG	1 cut(s)	1915
PstNI	CAGNNNCTG	2 cut(s)	821, 1043
PsuI	RGATCY	2 cut(s)	1921, 1985
PvuII	CAGCTG	2 cut(s)	1613, 2221
RruI	TCGCGA	1 cut(s)	2613
RsaI	GTAC	4 cut(s)	971, 1077, 1516, 2199
RsaNI	GTAC	4 cut(s)	970, 1076, 1515, 2198
RseI	CAYNNNNRTG	5 cut(s)	876, 1005, 1056, 1126, 2427
SacI	GAGCTC	2 cut(s)	716, 1890
SalI	GTCGAC	1 cut(s)	490
Sau3AI	GATC	9 cut(s)	238, 406, 823, 1267, 1310, 1723, 1921, 1985, 2343
Sau96I	GGNCC	6 cut(s)	59, 564, 600, 1157, 1898, 2567
SchI	GAGTC	4 cut(s)	948, 1241, 1346, 1748
ScrFI	CCNGG	6 cut(s)	442, 454, 864, 900, 1212, 1996
SduI	GDGCHC	7 cut(s)	716, 1108, 1451, 1890, 2063, 2491, 2632
SexAI	ACCWGGT	1 cut(s)	1210
SfaNI	GCATC	8 cut(s)	205, 265, 373, 682, 1108, 1616, 2029, 2293
SfcI	CTRYAG	3 cut(s)	1807, 1911, 2052
SinI	GGWCC	3 cut(s)	600, 1898, 2567
SmiMI	CAYNNNNRTG	5 cut(s)	876, 1005, 1056, 1126, 2427
SmlI	CTYRAG	4 cut(s)	1738, 2502, 2537, 2744
SmoI	CTYRAG	4 cut(s)	1738, 2502, 2537, 2744
SpeI	ACTAGT	1 cut(s)	605
SphI	GCATGC	1 cut(s)	2655
SsiI	CCGC	5 cut(s)	8, 562, 575, 923, 1944
SspI	AATATT	3 cut(s)	1144, 1183, 1861
SspMI	CTAG	8 cut(s)	20, 219, 606, 732, 741, 851, 1629, 2384
SstI	GAGCTC	2 cut(s)	716, 1890
StyD4I	CCNGG	6 cut(s)	440, 452, 862, 898, 1210, 1994
StyI	CCWWGG	2 cut(s)	1068, 2400
TaaI	ACNGT	6 cut(s)	52, 403, 1139, 1192, 1514, 2257
TaiI	ACGT	3 cut(s)	1679, 2355, 2494
TaqI	TCGA	2 cut(s)	314, 491
TatI	WGTACW	2 cut(s)	969, 1514
TauI	GCSGC	2 cut(s)	578, 1946
TfiI	GAWTC	8 cut(s)	412, 551, 755, 1034, 1431, 1454, 1526, 2754
TscAI	CASTG	8 cut(s)	1089, 1389, 1420, 1451, 1755, 1851, 1887, 1954
TseFI	GTSAC	8 cut(s)	459, 811, 905, 1233, 1415, 1672, 2418, 2512
Tsp45I	GTSAC	8 cut(s)	459, 811, 905, 1233, 1415, 1672, 2418, 2512
TspGWI	ACGGA	3 cut(s)	341, 1330, 1466
TspRI	CASTG	8 cut(s)	1089, 1389, 1420, 1451, 1755, 1851, 1887, 1954
Van91I	CCANNNNNTGG	1 cut(s)	1692
Vha464I	CTTAAG	2 cut(s)	2537, 2744
VneI	GTGCAC	3 cut(s)	1104, 1447, 2487
VpaK11BI	GGWCC	3 cut(s)	600, 1898, 2567
VspI	ATTAAT	1 cut(s)	198
XagI	CCTNNNNNAGG	2 cut(s)	587, 2540
XapI	RAATTY	9 cut(s)	146, 391, 465, 623, 831, 1201, 2310, 2437, 2639
XbaI	TCTAGA	1 cut(s)	731
XceI	RCATGY	5 cut(s)	232, 1055, 1246, 1693, 2655
XcmI	CCANNNNNNNNNTGG	1 cut(s)	74
XmiI	GTMKAC	3 cut(s)	45, 491, 1476
XmnI	GAANNNNTTC	1 cut(s)	1404
XspI	CTAG	8 cut(s)	20, 219, 606, 732, 741, 851, 1629, 2384

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.