Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002973

Physical Location & Seq

Genomic Coordinates Reverse (-)

5805978 .. 5812197

Gene Structure 6220 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1084000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2883 bp

ATGTATGCGGTTGGGAGGCTAGGGAGTTATATATCCCGAGGCGTTGTACACGCCTCCGGCCCGTTCCACCCGTTTGGTGGAGCTGTGGATATAATTGTAGTTGAACAACAAGATGGGAGCTTCAAATCGTCGGCGTGGAATGTGAAATTTGGTAAGTTTCAAGGGGTTTTGAAGACAAAGGAGAAGGTGGTAAACATTAATGTCAATGGGGAGGATGCTAGTTTCCATATGTATTTGGATCATAAAGGAGAAGCTTATTTTCTTAGGGAGGTTGATGCAGAGGAAGGGGAAGCTGTGTTGTATCCTTCGTCTTCGAGTGATGAGACGGATGAGCAATGTCGAGAAAACGGGCAGCCATTGAAGTCCATGAGTTGCAATTATGATGCTGAGAATTTGACACCAGTTGGTCAGATTGAAACGCCTAATGGGAAGATTTTGTCCAGGACTAATTCCCGGAGGTCAAGAATTTTAGGGTTATTTGGGAGCAGGTCGATGAAGGAGCGCAAGAGTAAGGAGGAAGAGGGGGTTGATAGCGGGGTGAGTAGGGTGGATTCGTTGGAGGGGGTGGAGTTTGCAGCAAACCTTTTGGAAGTGAATTGGTCCACTAGTCTTGCCATTAAGAAACCCGGGAAGGACAATGCTTCCCCTTTTTTGTCTCCTAATATGTTGGTTAATGAAGATATGCAGATTGATGCCGAGCAAAGCCAGTTGAGCTTGTCTCTGCCTGTGTCTAGAGTATCTAGCGACGTTGATGAATCTATTATTGCGGCAACTGAACATGATGGGAATGCAATGGCTGTCATATCCAATGCCACAGGTTCTGATCCGGAAATTCAAAGTTTGGTTGAGCTAGAAGCTTGCCCTGGCAAGCATTTGGATGAGAAAGATGTTGTATTACCTGGCTGTGGCATTTCTCATACAGCCGGTGGGATAGATCGAGTCCAACCCTTTATTTATTGTGAAACATTGTACATTGCCAGAGAAGGAAGTGAGCAAGTTCATGCTGAAACAGTGCATGCCAGAACTGAGCTGCTGTCTAAGGATTCAGTTACTGAGCAACATGCTGATGATACTTCCGTAGAGGTACAATCAGTGGAGGCTCCTGAAATTTGTTTACAAGAGACAGATACACATTCATGCACAGACCGTAATGCGAGCCGTGAAGGGCCAGCAATAGTTCAACCATGCAATAGTCAAACAGTCCAGCAAAATTCATTACCTGATTCGGTTCAAAAAGTTGAGTCACAGAGCATGTCTAGTTTAAACACCTATCGTGATCAAGTTCAAGAGGAGGAAAACATAGAAGAGGAAGATGTCGCATCAAGTGATTTACAAACCCCTTGTAAGTCCGTTAATGGCCATGTTGTGACAGAAGCAGCAAGTGTGTTGCCAGTGAGACTAGAGGAAGAACAATTCCTCTTCAGTGATGATGAAATCAGAGTCACTGAGGTTCAGTGCATGGAGTCACATTTTCCATCATGTGTAGGCGGAGAAAATTGTCTCTCGTGCAGTCCAAAAGACAGTACAAATTATGAATCATATCCATCTCTGGAAAAGTTTATTCCAGAAGATCCCTCAACTGATTTTCAGAAATCAACTGGAAAACTGAAGGCAGCATCAGCTGCAGTTGGTATTCCGAGAAATCATACGGCTGCTGCTGGCAAGGAAGTTGGGAGGCTGGTCGAATCCTTACCTATCATGTGGCCTCAGACTGACAAGTTGACTGCATTTGATGTTCATTATCCATTAAGCCATTCTCTGGACTCAAATGTCCATCCCTTGAAGTCGATATGGCAATGTAAAGATGATTTGAGCTTTAGAAAGTTTGACAGAGAAGAAGAGCAACAATTAGCACTGGAAACACCAGATAGCGAGAATGCTCCAGGTTCAGCGGAGCTCAAAGATGGTCCTACCAGTCCTGCAGTTGGAGATCTATCCAAAGCAAATGTTGCCGCCAGTGGAAGTTGGAAACTCTGGCCTTTCGGTTTTAGGGGATCAAATTCCAGGACAGCAATGCAGCCAGATGTGAATGGCAGTAAAAGTCCTGAAGCTGAGAATGCTTCAGAGAGCACCGTTGGCACAGATGGAGATAAGAATATGCTTACACCCAAGGGGATGAAGAGAATGGAAAGGGCACTCGCTCCAATGTCTGAGCAGCTGGCTTCCTTGAAACTGAAGGAAGGGAAGAATACAGTAACCTTCACATTCTTTACTGCAATGCTGGGGAAGCAGCAGGTTGATGCCAGAATATATCTGTGGAAATGGAACACACGCATAGTGATCTCAGATGTGGATGGGACAATCACAAAGTCAGATGTTCTAGGTCAATTCATGCCCTTGGTTGGGGTAGATTGGTCACATGTAGGTGTTACACATTTGTTTTCAGCCATTAAGGAAAATGGATATCAATTGCTTTTTTTGAGTGCACGTGCCATTTCTCAAGCCTCTCTCACCAGACAATTTTTATTCAACCTTAAGCAGGATGGGAAGGCTTTACCAGATGGTCCCGTTGTTATTTCCCCAGATGGACTTTTTCCTTCTCTCTTTCGCGAAGTTATCAGGAGAGCTCCTCATGAATTCAAGATCGCATGCTTAGAGGATATCAAATCATTGTTCCCTTCTGATTGCAATCCATTCTATGCTGGTTTTGGAAACAGAGATACCGATGAGTTTAGCTACCTTAAGGTTGGAATCCCGAAAGGAAAAATATTCATTATTAATCCCCAGGGTGAGGTTGCTGTGAACCGCCGAGTTGACACAAAGTCATATAGCTCCCTTCATGCTCTTGTGAATGGCATGTTTCCATCCACGAACTCATCTGAGCAGGAGGACTTTAATACGTGGAATTTCTGGAAACTCCCCCTGCCCGATATCATTGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006629 GO:0006643 GO:0006644 GO:0006664 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009058 GO:0009247 GO:0009987 GO:0016020 GO:0019374 GO:0019375 GO:0019637 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0046467 GO:0048046 GO:0071704 GO:0090407 GO:1901135 GO:1901137 GO:1901576 GO:1903509

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 map00561 map00564 map01100 map01110

Pfam Domains

Protein Families

LNS2 Lipin_N Lipin_mid

Protein Analysis

961

Amino Acids

105.42

Weight (kDa)

4.88

Isoelectric Point (pI)

44.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lipin_N	PF04571	12 - 97	1.2e-26	lipin, N-terminal conserved region
LNS2	PF08235	712 - 935	2.7e-95	LNS2 (Lipin/Ned1/Smp2)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002973)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G42870 AT5G42870
fragaria_vesca	FvH4_2g34540 FvH4_2g34540 FvH4_2g34540 FvH4_2g34540 FvH4_2g34540 FvH4_2g34540 FvH4_2g34540 FvH4_2g34540 FvH4_2g34540 FvH4_2g34540 FvH4_2g34540 FvH4_2g34540 FvH4_2g34540 FvH4_2g34540 FvH4_2g34540 FvH4_2g34540 FvH4_2g34540 FvH4_2g34540
malus_domestica	MD15G1030100.v1.1 MD15G1084000.v1.1
prunus_persica	Prupe.1G437400_v2.0.a1 Prupe.1G437400_v2.0.a1 Prupe.1G437400_v2.0.a1
pyrus_communis	pycom08g08400 pycom15g07920
rosa_chinensis	RchiOBHm_Chr6g0307611 RchiOBHm_Chr6g0307781
rosa_laevigata	RLG00000010701
rosa_multiflora	Rmu_sc0000031.1_g000031
rosa_roxburghii	Rroxscaffold_7G00160890
rosa_rugosa	Rorug06G0360900
rosa_samantha	Rh6AG469900 Rh6BG480400 Rh6CG486100 Rh6DG472500
rosa_wichuraiana	Rw6G041030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1769
Acc36I	ACCTGC	2 cut(s)	477, 2224
AccB7I	CCANNNNNTGG	1 cut(s)	1759
AccII	CGCG	1 cut(s)	2550
AccIII	TCCGGA	1 cut(s)	826
AciI	CCGC	7 cut(s)	8, 534, 767, 1488, 1892, 1953, 2746
AclWI	GGATC	4 cut(s)	246, 818, 1565, 2002
AcoI	YGGCCR	1 cut(s)	1357
AcsI	RAATTY	9 cut(s)	146, 391, 465, 831, 1107, 1210, 1999, 2576, 2845
AcuI	CTGAAG	5 cut(s)	1405, 1628, 2046, 2067, 2195
AcvI	CACGTG	1 cut(s)	2429
AfaI	GTAC	4 cut(s)	48, 971, 1086, 1525
AfiI	CCNNNNNNNGG	8 cut(s)	77, 905, 1759, 1925, 2342, 2343, 2479, 2731
AflII	CTTAAG	2 cut(s)	2474, 2681
AflIII	ACRYGT	1 cut(s)	2359
AhdI	GACNNNNNGTC	1 cut(s)	2761
AhlI	ACTAGT	1 cut(s)	605
AjnI	CCWGG	6 cut(s)	440, 862, 898, 1882, 2003, 2724
Alw21I	GWGCWC	4 cut(s)	1899, 2072, 2428, 2569
Alw26I	GTCTC	6 cut(s)	317, 660, 723, 1115, 1390, 1505
Alw44I	GTGCAC	1 cut(s)	2424
AlwI	GGATC	4 cut(s)	246, 818, 1565, 2002
AlwNI	CAGNNNCTG	2 cut(s)	821, 1052
Ama87I	CYCGRG	2 cut(s)	36, 626
Aor13HI	TCCGGA	1 cut(s)	826
AoxI	GGCC	5 cut(s)	58, 1166, 1357, 1703, 1976
ApaLI	GTGCAC	1 cut(s)	2424
ApoI	RAATTY	9 cut(s)	146, 391, 465, 831, 1107, 1210, 1999, 2576, 2845
AseI	ATTAAT	2 cut(s)	198, 2718
AspLEI	GCGC	1 cut(s)	504
AspS9I	GGNCC	5 cut(s)	59, 600, 1166, 1907, 2504
AsuC2I	CCSGG	3 cut(s)	454, 627, 628
AsuHPI	GGTGA	3 cut(s)	550, 2443, 2741
AvaI	CYCGRG	2 cut(s)	36, 626
AvaII	GGWCC	3 cut(s)	600, 1907, 2504
BaeGI	GKGCMC	2 cut(s)	2137, 2428
BaeI	ACNNNNGTAYC	2 cut(s)	1062, 1095
BalI	TGGCCA	1 cut(s)	1359
BanII	GRGCYC	2 cut(s)	1899, 2569
BauI	CACGAG	1 cut(s)	1504
BbrPI	CACGTG	1 cut(s)	2429
BbsI	GAAGAC	2 cut(s)	179, 303
Bbv12I	GWGCWC	4 cut(s)	1899, 2072, 2428, 2569
BceAI	ACGGC	2 cut(s)	1143, 1665
BciT130I	CCWGG	6 cut(s)	442, 864, 900, 1884, 2005, 2726
BciVI	GTATCC	1 cut(s)	312
BclI	TGATCA	1 cut(s)	1276
BcnI	CCSGG	3 cut(s)	454, 627, 628
BcoDI	GTCTC	6 cut(s)	317, 660, 723, 1115, 1390, 1505
BcuI	ACTAGT	1 cut(s)	605
BfaI	CTAG	9 cut(s)	20, 219, 606, 732, 741, 851, 1257, 1400, 2321
BfmI	CTRYAG	2 cut(s)	1623, 1920
BfrI	CTTAAG	2 cut(s)	2474, 2681
BfuAI	ACCTGC	2 cut(s)	477, 2224
BfuI	GTATCC	1 cut(s)	312
BglII	AGATCT	1 cut(s)	1930
Bme1390I	CCNGG	9 cut(s)	442, 454, 627, 628, 864, 900, 1884, 2005, 2726
Bme18I	GGWCC	3 cut(s)	600, 1907, 2504
BmeRI	GACNNNNNGTC	1 cut(s)	2761
BmeT110I	CYCGRG	2 cut(s)	36, 626
BmgT120I	GGNCC	5 cut(s)	59, 600, 1166, 1907, 2504
BmiI	GGNNCC	2 cut(s)	1101, 2506
BmrFI	CCNGG	9 cut(s)	442, 454, 627, 628, 864, 900, 1884, 2005, 2726
BmsI	GCATC	7 cut(s)	205, 265, 373, 682, 1328, 1625, 2230
BpiI	GAAGAC	2 cut(s)	179, 303
BplI	GAGNNNNNCTC	4 cut(s)	703, 735, 2554, 2586
BpmI	CTGGAG	1 cut(s)	1866
BpuEI	CTTGAG	1 cut(s)	2424
BpuMI	CCSGG	3 cut(s)	454, 627, 628
BsaAI	YACGTR	2 cut(s)	2429, 2841
BsaBI	GATNNNNATC	1 cut(s)	2628
BsaJI	CCNNGG	7 cut(s)	37, 626, 862, 2109, 2337, 2724, 2725
BsaWI	WCCGGW	1 cut(s)	826
Bsc4I	CCNNNNNNNGG	8 cut(s)	77, 905, 1759, 1925, 2342, 2343, 2479, 2731
Bse118I	RCCGGY	1 cut(s)	923
Bse1I	ACTGG	7 cut(s)	401, 706, 1391, 1603, 1860, 1914, 1956
Bse3DI	GCAATG	6 cut(s)	341, 798, 972, 1802, 2019, 2223
Bse8I	GATNNNNATC	1 cut(s)	2628
BseAI	TCCGGA	1 cut(s)	826
BseBI	CCWGG	6 cut(s)	442, 864, 900, 1884, 2005, 2726
BseDI	CCNNGG	7 cut(s)	37, 626, 862, 2109, 2337, 2724, 2725
BseGI	GGATG	8 cut(s)	220, 334, 883, 1774, 2121, 2299, 2488, 2804
BseJI	GATNNNNATC	1 cut(s)	2628
BseLI	CCNNNNNNNGG	8 cut(s)	77, 905, 1759, 1925, 2342, 2343, 2479, 2731
BseMI	GCAATG	6 cut(s)	341, 798, 972, 1802, 2019, 2223
BseMII	CTCAG	9 cut(s)	378, 1017, 1044, 1437, 1721, 2043, 2142, 2298, 2811
BseNI	ACTGG	7 cut(s)	401, 706, 1391, 1603, 1860, 1914, 1956
BseRI	GAGGAG	2 cut(s)	1304, 2559
BseSI	GKGCMC	2 cut(s)	2137, 2428
BseYI	CCCAGC	1 cut(s)	2221
BsgI	GTGCAG	1 cut(s)	1528
Bsh1236I	CGCG	1 cut(s)	2550
BshFI	GGCC	5 cut(s)	60, 1168, 1359, 1705, 1978
BsiHKAI	GWGCWC	4 cut(s)	1899, 2072, 2428, 2569
BsiHKCI	CYCGRG	2 cut(s)	36, 626
BsiSI	CCGG	5 cut(s)	57, 454, 627, 827, 924
BslFI	GGGAC	2 cut(s)	2311, 2490
BslI	CCNNNNNNNGG	8 cut(s)	77, 905, 1759, 1925, 2342, 2343, 2479, 2731
BsmAI	GTCTC	6 cut(s)	317, 660, 723, 1115, 1390, 1505
BsmBI	CGTCTC	1 cut(s)	317
BsmFI	GGGAC	2 cut(s)	2311, 2490
BsmI	GAATGC	3 cut(s)	793, 1882, 2062
BsnI	GGCC	5 cut(s)	60, 1168, 1359, 1705, 1978
BsoBI	CYCGRG	2 cut(s)	36, 626
Bsp1286I	GDGCHC	5 cut(s)	1899, 2072, 2137, 2428, 2569
Bsp13I	TCCGGA	1 cut(s)	826
Bsp1407I	TGTACA	2 cut(s)	46, 969
Bsp143I	GATC	9 cut(s)	238, 823, 934, 1276, 1570, 1930, 1994, 2280, 2583
Bsp68I	TCGCGA	1 cut(s)	2550
BspACI	CCGC	7 cut(s)	8, 534, 767, 1488, 1892, 1953, 2746
BspANI	GGCC	5 cut(s)	60, 1168, 1359, 1705, 1978
BspCNI	CTCAG	9 cut(s)	379, 1018, 1045, 1438, 1720, 2044, 2143, 2297, 2812
BspEI	TCCGGA	1 cut(s)	826
BspFNI	CGCG	1 cut(s)	2550
BspHI	TCATGA	1 cut(s)	2572
BspLI	GGNNCC	2 cut(s)	1101, 2506
BspMAI	CTGCAG	2 cut(s)	1627, 1924
BspMI	ACCTGC	2 cut(s)	477, 2224
BspPI	GGATC	4 cut(s)	246, 818, 1565, 2002
BspQI	GCTCTTC	1 cut(s)	1833
BspTI	CTTAAG	2 cut(s)	2474, 2681
BsrDI	GCAATG	6 cut(s)	341, 798, 972, 1802, 2019, 2223
BsrFI	RCCGGY	1 cut(s)	923
BsrGI	TGTACA	2 cut(s)	46, 969
BsrI	ACTGG	7 cut(s)	401, 706, 1391, 1603, 1860, 1914, 1956
BssAI	RCCGGY	1 cut(s)	923
BssECI	CCNNGG	7 cut(s)	37, 626, 862, 2109, 2337, 2724, 2725
BssMI	GATC	9 cut(s)	238, 823, 934, 1276, 1570, 1930, 1994, 2280, 2583
BssSI	CACGAG	1 cut(s)	1504
BssT1I	CCWWGG	2 cut(s)	2109, 2337
Bst2BI	CACGAG	1 cut(s)	1504
Bst2UI	CCWGG	6 cut(s)	442, 864, 900, 1884, 2005, 2726
Bst4CI	ACNGT	6 cut(s)	1012, 1148, 1201, 1523, 2074, 2194
Bst6I	CTCTTC	5 cut(s)	513, 1299, 1424, 1833, 2114
BstAFI	CTTAAG	2 cut(s)	2474, 2681
BstAPI	GCANNNNNTGC	2 cut(s)	1622, 1949
BstAUI	TGTACA	2 cut(s)	46, 969
BstBAI	YACGTR	2 cut(s)	2429, 2841
BstC8I	GCNNGC	8 cut(s)	859, 869, 1017, 1156, 1170, 1660, 2160, 2590
BstENI	CCTNNNNNAGG	1 cut(s)	2477
BstF5I	GGATG	8 cut(s)	220, 334, 883, 1774, 2121, 2299, 2488, 2804
BstFNI	CGCG	1 cut(s)	2550
BstHHI	GCGC	1 cut(s)	504
BstKTI	GATC	9 cut(s)	241, 826, 937, 1279, 1573, 1933, 1997, 2283, 2586
BstMAI	GTCTC	6 cut(s)	317, 660, 723, 1115, 1390, 1505
BstMBI	GATC	9 cut(s)	238, 823, 934, 1276, 1570, 1930, 1994, 2280, 2583
BstMWI	GCNNNNNNNGC	7 cut(s)	711, 1619, 1622, 1949, 2057, 2076, 2227
BstNI	CCWGG	6 cut(s)	442, 864, 900, 1884, 2005, 2726
BstNSI	RCATGY	6 cut(s)	1019, 1064, 1255, 2363, 2592, 2800
BstSCI	CCNGG	9 cut(s)	440, 452, 625, 626, 862, 898, 1882, 2003, 2724
BstSFI	CTRYAG	2 cut(s)	1623, 1920
BstSLI	GKGCMC	2 cut(s)	2137, 2428
BstUI	CGCG	1 cut(s)	2550
BstV2I	GAAGAC	2 cut(s)	179, 303
BstX2I	RGATCY	2 cut(s)	1570, 1930
BstXI	CCANNNNNNTGG	1 cut(s)	74
BstYI	RGATCY	2 cut(s)	1570, 1930
BsuI	GTATCC	1 cut(s)	312
BsuRI	GGCC	5 cut(s)	60, 1168, 1359, 1705, 1978
BtsCI	GGATG	8 cut(s)	220, 334, 883, 1774, 2121, 2299, 2488, 2804
BtsIMutI	CAGTG	8 cut(s)	1017, 1098, 1398, 1429, 1443, 1460, 1853, 1963
BtuMI	TCGCGA	1 cut(s)	2550
BveI	ACCTGC	2 cut(s)	477, 2224
Cac8I	GCNNGC	8 cut(s)	859, 869, 1017, 1156, 1170, 1660, 2160, 2590
CaiI	CAGNNNCTG	2 cut(s)	821, 1052
CciI	TCATGA	1 cut(s)	2572
CfoI	GCGC	1 cut(s)	504
Cfr10I	RCCGGY	1 cut(s)	923
Cfr13I	GGNCC	5 cut(s)	59, 600, 1166, 1907, 2504
Cfr9I	CCCGGG	1 cut(s)	626
Csp6I	GTAC	4 cut(s)	47, 970, 1085, 1524
CspCI	CAANNNNNGTGG	2 cut(s)	592, 627
CviQI	GTAC	4 cut(s)	47, 970, 1085, 1524
DpnI	GATC	9 cut(s)	240, 825, 936, 1278, 1572, 1932, 1996, 2282, 2585
DpnII	GATC	9 cut(s)	238, 823, 934, 1276, 1570, 1930, 1994, 2280, 2583
DraI	TTTAAA	1 cut(s)	1263
DrdI	GACNNNNNNGTC	1 cut(s)	1769
DriI	GACNNNNNGTC	1 cut(s)	2761
DseDI	GACNNNNNNGTC	1 cut(s)	1769
EaeI	YGGCCR	1 cut(s)	1357
Eam1104I	CTCTTC	5 cut(s)	513, 1299, 1424, 1833, 2114
Eam1105I	GACNNNNNGTC	1 cut(s)	2761
EarI	CTCTTC	5 cut(s)	513, 1299, 1424, 1833, 2114
EciI	GGCGGA	1 cut(s)	1503
Ecl136II	GAGCTC	2 cut(s)	1897, 2567
Eco130I	CCWWGG	2 cut(s)	2109, 2337
Eco24I	GRGCYC	2 cut(s)	1899, 2569
Eco32I	GATATC	3 cut(s)	2405, 2602, 2872
Eco47I	GGWCC	3 cut(s)	600, 1907, 2504
Eco53kI	GAGCTC	2 cut(s)	1897, 2567
Eco57I	CTGAAG	5 cut(s)	1405, 1628, 2046, 2067, 2195
Eco72I	CACGTG	1 cut(s)	2429
Eco88I	CYCGRG	2 cut(s)	36, 626
EcoICRI	GAGCTC	2 cut(s)	1897, 2567
EcoNI	CCTNNNNNAGG	1 cut(s)	2477
EcoRI	GAATTC	1 cut(s)	2576
EcoRII	CCWGG	6 cut(s)	440, 862, 898, 1882, 2003, 2724
EcoRV	GATATC	3 cut(s)	2405, 2602, 2872
EcoT14I	CCWWGG	2 cut(s)	2109, 2337
EcoT38I	GRGCYC	2 cut(s)	1899, 2569
ErhI	CCWWGG	2 cut(s)	2109, 2337
Esp3I	CGTCTC	1 cut(s)	317
FaqI	GGGAC	2 cut(s)	2311, 2490
FauI	CCCGC	1 cut(s)	527
FauNDI	CATATG	1 cut(s)	228
FbaI	TGATCA	1 cut(s)	1276
FokI	GGATG	8 cut(s)	227, 341, 890, 1761, 2128, 2306, 2495, 2791
FriOI	GRGCYC	2 cut(s)	1899, 2569
FspBI	CTAG	9 cut(s)	20, 219, 606, 732, 741, 851, 1257, 1400, 2321
GlaI	GCGC	1 cut(s)	503
GsaI	CCCAGC	1 cut(s)	2225
GsuI	CTGGAG	1 cut(s)	1866
HaeIII	GGCC	5 cut(s)	60, 1168, 1359, 1705, 1978
HapII	CCGG	5 cut(s)	57, 454, 627, 827, 924
HhaI	GCGC	1 cut(s)	504
Hin6I	GCGC	1 cut(s)	502
HinP1I	GCGC	1 cut(s)	502
HincII	GTYRAC	2 cut(s)	1722, 2755
HindII	GTYRAC	2 cut(s)	1722, 2755
HindIII	AAGCTT	2 cut(s)	252, 855
HpaII	CCGG	5 cut(s)	57, 454, 627, 827, 924
HphI	GGTGA	3 cut(s)	550, 2443, 2741
Hpy166II	GTNNAC	8 cut(s)	49, 193, 603, 1115, 1722, 2426, 2743, 2755
Hpy8I	GTNNAC	8 cut(s)	49, 193, 603, 1115, 1722, 2426, 2743, 2755
Hpy99I	CGWCG	2 cut(s)	133, 749
HpyCH4III	ACNGT	6 cut(s)	1012, 1148, 1201, 1523, 2074, 2194
HpyCH4IV	ACGT	3 cut(s)	747, 2428, 2840
HpyF10VI	GCNNNNNNNGC	7 cut(s)	711, 1619, 1622, 1949, 2057, 2076, 2227
HpySE526I	ACGT	3 cut(s)	747, 2428, 2840
HspAI	GCGC	1 cut(s)	502
Kpn2I	TCCGGA	1 cut(s)	826
Ksp22I	TGATCA	1 cut(s)	1276
Kzo9I	GATC	9 cut(s)	238, 823, 934, 1276, 1570, 1930, 1994, 2280, 2583
LguI	GCTCTTC	1 cut(s)	1833
LweI	GCATC	7 cut(s)	205, 265, 373, 682, 1328, 1625, 2230
MaeI	CTAG	9 cut(s)	20, 219, 606, 732, 741, 851, 1257, 1400, 2321
MaeII	ACGT	3 cut(s)	747, 2428, 2840
MaeIII	GTNAC	8 cut(s)	1048, 1242, 1366, 1441, 1464, 2194, 2355, 2368
MalI	GATC	9 cut(s)	240, 825, 936, 1278, 1572, 1932, 1996, 2282, 2585
MboI	GATC	9 cut(s)	238, 823, 934, 1276, 1570, 1930, 1994, 2280, 2583
MfeI	CAATTG	1 cut(s)	2408
MflI	RGATCY	2 cut(s)	1570, 1930
MhlI	GDGCHC	5 cut(s)	1899, 2072, 2137, 2428, 2569
MlsI	TGGCCA	1 cut(s)	1359
MluNI	TGGCCA	1 cut(s)	1359
MlyI	GAGTC	5 cut(s)	948, 1250, 1449, 1472, 1757
MmeI	TCCRAC	5 cut(s)	537, 967, 1906, 1946, 2668
Mox20I	TGGCCA	1 cut(s)	1359
MroI	TCCGGA	1 cut(s)	826
MscI	TGGCCA	1 cut(s)	1359
MslI	CAYNNNNRTG	4 cut(s)	876, 1065, 1135, 2364
Msp20I	TGGCCA	1 cut(s)	1359
MspA1I	CMGCKG	3 cut(s)	1622, 1892, 2158
MspCI	CTTAAG	2 cut(s)	2474, 2681
MspI	CCGG	5 cut(s)	57, 454, 627, 827, 924
MspR9I	CCNGG	9 cut(s)	442, 454, 627, 628, 864, 900, 1884, 2005, 2726
MssI	GTTTAAAC	1 cut(s)	1263
MunI	CAATTG	1 cut(s)	2408
Mva1269I	GAATGC	3 cut(s)	793, 1882, 2062
MvaI	CCWGG	6 cut(s)	442, 864, 900, 1884, 2005, 2726
MvnI	CGCG	1 cut(s)	2550
MwoI	GCNNNNNNNGC	7 cut(s)	711, 1619, 1622, 1949, 2057, 2076, 2227
NciI	CCSGG	3 cut(s)	454, 627, 628
NdeI	CATATG	1 cut(s)	228
NdeII	GATC	9 cut(s)	238, 823, 934, 1276, 1570, 1930, 1994, 2280, 2583
NlaIV	GGNNCC	2 cut(s)	1101, 2506
NmeAIII	GCCGAG	2 cut(s)	721, 2774
NmuCI	GTSAC	5 cut(s)	1242, 1366, 1441, 1464, 2355
NruI	TCGCGA	1 cut(s)	2550
NspI	RCATGY	6 cut(s)	1019, 1064, 1255, 2363, 2592, 2800
PaeI	GCATGC	2 cut(s)	1019, 2592
PagI	TCATGA	1 cut(s)	2572
PasI	CCCWGGG	1 cut(s)	2725
PciI	ACATGT	1 cut(s)	2359
PciSI	GCTCTTC	1 cut(s)	1833
PctI	GAATGC	3 cut(s)	793, 1882, 2062
PfeI	GAWTC	7 cut(s)	551, 755, 1043, 1223, 1535, 1685, 2691
PflMI	CCANNNNNTGG	1 cut(s)	1759
PfoI	TCCNGGA	3 cut(s)	440, 452, 2003
PleI	GAGTC	5 cut(s)	947, 1249, 1448, 1471, 1757
PmaCI	CACGTG	1 cut(s)	2429
PmeI	GTTTAAAC	1 cut(s)	1263
PmlI	CACGTG	1 cut(s)	2429
PpsI	GAGTC	5 cut(s)	947, 1249, 1448, 1471, 1757
Ppu21I	YACGTR	2 cut(s)	2429, 2841
PscI	ACATGT	1 cut(s)	2359
PshBI	ATTAAT	2 cut(s)	198, 2718
Psp124BI	GAGCTC	2 cut(s)	1899, 2569
Psp6I	CCWGG	6 cut(s)	440, 862, 898, 1882, 2003, 2724
PspCI	CACGTG	1 cut(s)	2429
PspFI	CCCAGC	1 cut(s)	2221
PspGI	CCWGG	6 cut(s)	440, 862, 898, 1882, 2003, 2724
PspN4I	GGNNCC	2 cut(s)	1101, 2506
PspPI	GGNCC	5 cut(s)	59, 600, 1166, 1907, 2504
PstI	CTGCAG	2 cut(s)	1627, 1924
PstNI	CAGNNNCTG	2 cut(s)	821, 1052
PsuI	RGATCY	2 cut(s)	1570, 1930
PvuII	CAGCTG	2 cut(s)	1622, 2158
RruI	TCGCGA	1 cut(s)	2550
RsaI	GTAC	4 cut(s)	48, 971, 1086, 1525
RsaNI	GTAC	4 cut(s)	47, 970, 1085, 1524
RseI	CAYNNNNRTG	4 cut(s)	876, 1065, 1135, 2364
SacI	GAGCTC	2 cut(s)	1899, 2569
SapI	GCTCTTC	1 cut(s)	1833
Sau3AI	GATC	9 cut(s)	238, 823, 934, 1276, 1570, 1930, 1994, 2280, 2583
Sau96I	GGNCC	5 cut(s)	59, 600, 1166, 1907, 2504
SchI	GAGTC	5 cut(s)	948, 1250, 1449, 1472, 1757
ScrFI	CCNGG	9 cut(s)	442, 454, 627, 628, 864, 900, 1884, 2005, 2726
SduI	GDGCHC	5 cut(s)	1899, 2072, 2137, 2428, 2569
SfaNI	GCATC	7 cut(s)	205, 265, 373, 682, 1328, 1625, 2230
SfcI	CTRYAG	2 cut(s)	1623, 1920
SinI	GGWCC	3 cut(s)	600, 1907, 2504
SmaI	CCCGGG	1 cut(s)	628
SmiMI	CAYNNNNRTG	4 cut(s)	876, 1065, 1135, 2364
SmlI	CTYRAG	3 cut(s)	2439, 2474, 2681
SmoI	CTYRAG	3 cut(s)	2439, 2474, 2681
SpeI	ACTAGT	1 cut(s)	605
SphI	GCATGC	2 cut(s)	1019, 2592
SsiI	CCGC	7 cut(s)	8, 534, 767, 1488, 1892, 1953, 2746
SspI	AATATT	1 cut(s)	2709
SspMI	CTAG	9 cut(s)	20, 219, 606, 732, 741, 851, 1257, 1400, 2321
SstI	GAGCTC	2 cut(s)	1899, 2569
StyD4I	CCNGG	9 cut(s)	440, 452, 625, 626, 862, 898, 1882, 2003, 2724
StyI	CCWWGG	2 cut(s)	2109, 2337
TaaI	ACNGT	6 cut(s)	1012, 1148, 1201, 1523, 2074, 2194
TaiI	ACGT	3 cut(s)	750, 2431, 2843
TaqI	TCGA	6 cut(s)	314, 340, 491, 937, 1683, 1787
TatI	WGTACW	3 cut(s)	46, 969, 1523
TauI	GCSGC	2 cut(s)	770, 1955
TfiI	GAWTC	7 cut(s)	551, 755, 1043, 1223, 1535, 1685, 2691
TscAI	CASTG	8 cut(s)	1017, 1098, 1398, 1429, 1450, 1460, 1860, 1963
TseFI	GTSAC	5 cut(s)	1242, 1366, 1441, 1464, 2355
Tsp45I	GTSAC	5 cut(s)	1242, 1366, 1441, 1464, 2355
TspGWI	ACGGA	3 cut(s)	341, 1066, 1339
TspMI	CCCGGG	1 cut(s)	626
TspRI	CASTG	8 cut(s)	1017, 1098, 1398, 1429, 1450, 1460, 1860, 1963
Van91I	CCANNNNNTGG	1 cut(s)	1759
Vha464I	CTTAAG	2 cut(s)	2474, 2681
VneI	GTGCAC	1 cut(s)	2424
VpaK11BI	GGWCC	3 cut(s)	600, 1907, 2504
VspI	ATTAAT	2 cut(s)	198, 2718
XagI	CCTNNNNNAGG	1 cut(s)	2477
XapI	RAATTY	9 cut(s)	146, 391, 465, 831, 1107, 1210, 1999, 2576, 2845
XbaI	TCTAGA	1 cut(s)	731
XceI	RCATGY	6 cut(s)	1019, 1064, 1255, 2363, 2592, 2800
XcmI	CCANNNNNNNNNTGG	1 cut(s)	74
XmaI	CCCGGG	1 cut(s)	626
XspI	CTAG	9 cut(s)	20, 219, 606, 732, 741, 851, 1257, 1400, 2321

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.