Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0003357

Physical Location & Seq

Genomic Coordinates Reverse (-)

3865294 .. 3867564

Gene Structure 2271 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1057700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1134 bp

ATGGATGGAGTGTTGGTGAAAGAGGAGGAGGTTGTGACATGCACCGTCGGCTCATCGTCTTCGTCGTCCTCAAGCTTCTCGCCGCAGCCAATAGAGGGGCTGCACGAGGTGGGTCCGCCGCCGTTTCTCACGAAGACGTTTGAAATGGTGGAGGATCCTTCCACGGACGCCATTGTTTCCTGGAGCAAAGCTCGCAACAGCTTCGTTGTGTGGGACTATCACAAGTTCTCCGCCACTCTTCTTCCTCGATACTTCAAGCACAGTAACTTCTCCAGCTTCATTCGTCAGCTCAATACATATGGTTTCAGAAAGGTTGATCCGGACCGATGGGAATTCGCAAACGAGGGTTTTCTAGGAGGGCAGAGGCATTTGCTGAAGACCATTAAGAGGAGGAGACATGTCTCGCAGAATATGCAACAAGAAGCCGGAGGAGGAGCTTGTGTTGAATTGGGGCAGTATGGAGTAGAAACCGAGCTTGAAAGACTGAAAAGAGACCGAAACATTTTGATGACGGAGATTGTGAAGTTGAGGCAGCAACAACAAAATTCAAGGGAGCAAGTCATGGCAATGGAGGGCCGGTTGCTGACCACGGAGAAAAAACAGCAGCAGATGATGACTTTCCTTGCTAAAGCACTCAATAATCCATCTTTTCTACAAACCCTTGCTGAGAAGAATGCCAAGAATAGAGCACTGCACGGTATTGGTAGAAAGCGGAGACTGGCTGCTAGTCCCAGTCTTGAGAATCTGCAAGAAGCGCCTAGAATCGACGTTGTGGACTACTCTGCAAGCCAGGATCTGGGAGAGTTGGCAACTATGGAACCAGATATTGAGAACTTTTTCTCAACTGCTGCATTGGACAACAAATCGACCAGTGGTTTCAAAGACCGTAAACCGAGCTTAGTACCCGCAAGTACTGGTGGAGGCAACTTGGATTCTGTTGATGAGACCATATGGGAGGAGCTGTGGAGTGATGACCTCATTGGTGGCAACCCGGAGGAGGAAGCTGCTGCGGTGGGCGAGGAATCAGAAGTCGACGTTGAGGTGGAGGATTTGGTTGCAGAGCCTGCAGATTGGGGTGAGGACTTGCAAGACCTTGTTGATCAAATGGGTTATCTAATCCGGCCCAAGCCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0001067 GO:0001666 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006979 GO:0006986 GO:0008150 GO:0009266 GO:0009314 GO:0009408 GO:0009416 GO:0009628 GO:0009636 GO:0009642 GO:0009644 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010035 GO:0010200 GO:0010243 GO:0010286 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0033554 GO:0034605 GO:0034620 GO:0035966 GO:0035967 GO:0036293 GO:0036294 GO:0042221 GO:0042493 GO:0042542 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044212 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0046677 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0065007 GO:0070482 GO:0070887 GO:0071310 GO:0071453 GO:0071456 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HSF_DNA-bind

Protein Analysis

378

Amino Acids

42.23

Weight (kDa)

4.9

Isoelectric Point (pI)

56.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HSF_DNA-bind	PF00447	42 - 131	2.9e-29	HSF-type DNA-binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003357)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G26150 AT2G26150 AT2G26150 AT2G26150
fragaria_vesca	FvH4_2g31690 FvH4_2g31690 FvH4_2g31690 FvH4_2g31690 FvH4_2g31690 FvH4_2g31690 FvH4_2g31690
malus_domestica	MD08G1064100.v1.1 MD15G1057700.v1.1
prunus_persica	Prupe.1G410400_v2.0.a1 Prupe.1G410400_v2.0.a1 Prupe.1G410400_v2.0.a1 Prupe.1G410400_v2.0.a1 Prupe.1G410400_v2.0.a1 Prupe.1G410400_v2.0.a1 Prupe.1G410400_v2.0.a1 Prupe.1G410400_v2.0.a1
pyrus_communis	pycom08g05260 pycom15g05430
rosa_chinensis	RchiOBHm_Chr6g0311151
rosa_laevigata	RLG00000010432
rosa_multiflora	Rmu_sc0008967.1_g000010
rosa_roxburghii	Rroxscaffold_7G00157980
rosa_rugosa	Rorug06G0385800 Rorug06G0385900 Rorug06G0385900
rosa_samantha	Rh2CG294900 Rh6AG499900 Rh6BG510600 Rh6CG516300 Rh6DG502800
rosa_wichuraiana	Rw6G043540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	796
AccI	GTMKAC	1 cut(s)	1032
AccIII	TCCGGA	1 cut(s)	319
AciI	CCGC	7 cut(s)	83, 116, 119, 231, 712, 906, 1010
AclWI	GGATC	4 cut(s)	149, 162, 311, 801
AcsI	RAATTY	2 cut(s)	332, 544
AcuI	CTGAAG	1 cut(s)	395
AcyI	GRCGYC	1 cut(s)	168
AdeI	CACNNNGTG	1 cut(s)	109
AfaI	GTAC	2 cut(s)	903, 913
AfiI	CCNNNNNNNGG	4 cut(s)	95, 387, 796, 997
AflIII	ACRYGT	1 cut(s)	397
AgsI	TTSAA	6 cut(s)	143, 256, 446, 479, 549, 880
AjnI	CCWGG	2 cut(s)	179, 789
Alw21I	GWGCWC	1 cut(s)	691
Alw26I	GTCTC	5 cut(s)	388, 406, 486, 709, 938
AlwI	GGATC	4 cut(s)	149, 162, 311, 801
AlwNI	CAGNNNCTG	2 cut(s)	796, 1064
Aor13HI	TCCGGA	1 cut(s)	319
AoxI	GGCC	2 cut(s)	574, 1121
ApeKI	GCWGC	8 cut(s)	85, 100, 532, 604, 722, 848, 1004, 1007
ApoI	RAATTY	2 cut(s)	332, 544
Asp700I	GAANNNNTTC	1 cut(s)	836
AspLEI	GCGC	1 cut(s)	757
AspS9I	GGNCC	4 cut(s)	113, 322, 574, 1122
AsuC2I	CCSGG	1 cut(s)	992
AsuHPI	GGTGA	2 cut(s)	28, 1088
AvaII	GGWCC	2 cut(s)	113, 322
BamHI	GGATCC	1 cut(s)	154
BauI	CACGAG	1 cut(s)	104
BbsI	GAAGAC	3 cut(s)	51, 140, 383
Bbv12I	GWGCWC	1 cut(s)	691
BbvI	GCAGC	8 cut(s)	87, 97, 544, 616, 709, 835, 991, 994
BccI	CCATC	2 cut(s)	321, 652
BceAI	ACGGC	2 cut(s)	106, 1114
BcgI	CGANNNNNNTGC	2 cut(s)	184, 218
BciT130I	CCWGG	2 cut(s)	181, 791
BclI	TGATCA	1 cut(s)	1099
BcnI	CCSGG	1 cut(s)	992
BcoDI	GTCTC	5 cut(s)	388, 406, 486, 709, 938
BfaI	CTAG	3 cut(s)	353, 726, 759
BfmI	CTRYAG	1 cut(s)	1065
BfoI	RGCGCY	1 cut(s)	758
BmcAI	AGTACT	1 cut(s)	913
Bme1390I	CCNGG	3 cut(s)	181, 791, 992
Bme18I	GGWCC	2 cut(s)	113, 322
BmgT120I	GGNCC	4 cut(s)	113, 322, 574, 1122
BmiI	GGNNCC	3 cut(s)	114, 156, 819
BmrFI	CCNGG	3 cut(s)	181, 791, 992
BmrI	ACTGGG	1 cut(s)	726
BmuI	ACTGGG	1 cut(s)	726
BpiI	GAAGAC	3 cut(s)	51, 140, 383
BplI	GAGNNNNNCTC	2 cut(s)	175, 207
BpmI	CTGGAG	2 cut(s)	202, 256
BpuEI	CTTGAG	2 cut(s)	55, 758
BpuMI	CCSGG	1 cut(s)	992
BsaHI	GRCGYC	1 cut(s)	168
BsaI	GGTCTC	2 cut(s)	486, 938
BsaJI	CCNNGG	2 cut(s)	162, 588
BsaWI	WCCGGW	1 cut(s)	319
BsaXI	ACNNNNNCTCC	9 cut(s)	30, 212, 242, 426, 456, 563, 593, 947, 977
Bsc4I	CCNNNNNNNGG	4 cut(s)	95, 387, 796, 997
Bse118I	RCCGGY	1 cut(s)	576
Bse1I	ACTGG	4 cut(s)	723, 732, 870, 919
Bse3DI	GCAATG	1 cut(s)	573
BseAI	TCCGGA	1 cut(s)	319
BseBI	CCWGG	2 cut(s)	181, 791
BseDI	CCNNGG	2 cut(s)	162, 588
BseGI	GGATG	1 cut(s)	10
BseLI	CCNNNNNNNGG	4 cut(s)	95, 387, 796, 997
BseMI	GCAATG	1 cut(s)	573
BseMII	CTCAG	1 cut(s)	657
BseNI	ACTGG	4 cut(s)	723, 732, 870, 919
BseRI	GAGGAG	8 cut(s)	38, 41, 403, 406, 444, 447, 971, 1010
BseXI	GCAGC	8 cut(s)	87, 97, 544, 616, 709, 835, 991, 994
BsgI	GTGCAG	2 cut(s)	86, 677
BshFI	GGCC	2 cut(s)	576, 1123
BsiHKAI	GWGCWC	1 cut(s)	691
BsiSI	CCGG	5 cut(s)	320, 426, 577, 992, 1120
BslFI	GGGAC	2 cut(s)	227, 714
BslI	CCNNNNNNNGG	4 cut(s)	95, 387, 796, 997
BsmAI	GTCTC	5 cut(s)	388, 406, 486, 709, 938
BsmFI	GGGAC	2 cut(s)	227, 714
BsmI	GAATGC	1 cut(s)	679
BsnI	GGCC	2 cut(s)	576, 1123
Bso31I	GGTCTC	2 cut(s)	486, 938
Bsp1286I	GDGCHC	1 cut(s)	691
Bsp13I	TCCGGA	1 cut(s)	319
Bsp143I	GATC	4 cut(s)	154, 316, 793, 1099
BspACI	CCGC	7 cut(s)	83, 116, 119, 231, 712, 906, 1010
BspANI	GGCC	2 cut(s)	576, 1123
BspCNI	CTCAG	1 cut(s)	658
BspEI	TCCGGA	1 cut(s)	319
BspLI	GGNNCC	3 cut(s)	114, 156, 819
BspMAI	CTGCAG	1 cut(s)	1069
BspPI	GGATC	4 cut(s)	149, 162, 311, 801
BspTNI	GGTCTC	2 cut(s)	486, 938
BsrDI	GCAATG	1 cut(s)	573
BsrFI	RCCGGY	1 cut(s)	576
BsrI	ACTGG	4 cut(s)	723, 732, 870, 919
BssAI	RCCGGY	1 cut(s)	576
BssECI	CCNNGG	2 cut(s)	162, 588
BssMI	GATC	4 cut(s)	154, 316, 793, 1099
BssNI	GRCGYC	1 cut(s)	168
BssSI	CACGAG	1 cut(s)	104
Bst2BI	CACGAG	1 cut(s)	104
Bst2UI	CCWGG	2 cut(s)	181, 791
Bst4CI	ACNGT	4 cut(s)	46, 263, 698, 887
Bst6I	CTCTTC	1 cut(s)	243
BstACI	GRCGYC	1 cut(s)	168
BstAPI	GCANNNNNTGC	2 cut(s)	412, 1064
BstC8I	GCNNGC	3 cut(s)	193, 787, 1065
BstDEI	CTNAG	2 cut(s)	666, 898
BstDSI	CCRYGG	2 cut(s)	162, 588
BstF5I	GGATG	1 cut(s)	10
BstH2I	RGCGCY	1 cut(s)	758
BstHHI	GCGC	1 cut(s)	757
BstKTI	GATC	4 cut(s)	157, 319, 796, 1102
BstMAI	GTCTC	5 cut(s)	388, 406, 486, 709, 938
BstMBI	GATC	4 cut(s)	154, 316, 793, 1099
BstMWI	GCNNNNNNNGC	5 cut(s)	48, 192, 412, 754, 1064
BstNI	CCWGG	2 cut(s)	181, 791
BstNSI	RCATGY	2 cut(s)	42, 401
BstSCI	CCNGG	3 cut(s)	179, 789, 990
BstSFI	CTRYAG	1 cut(s)	1065
BstV1I	GCAGC	8 cut(s)	87, 97, 544, 616, 709, 835, 991, 994
BstV2I	GAAGAC	3 cut(s)	51, 140, 383
BstX2I	RGATCY	2 cut(s)	154, 793
BstYI	RGATCY	2 cut(s)	154, 793
BsuRI	GGCC	2 cut(s)	576, 1123
BtgI	CCRYGG	2 cut(s)	162, 588
BtsCI	GGATG	1 cut(s)	10
BtsI	GCAGTG	1 cut(s)	689
BtsIMutI	CAGTG	2 cut(s)	689, 877
Cac8I	GCNNGC	3 cut(s)	193, 787, 1065
CaiI	CAGNNNCTG	2 cut(s)	796, 1064
CfoI	GCGC	1 cut(s)	757
Cfr10I	RCCGGY	1 cut(s)	576
Cfr13I	GGNCC	4 cut(s)	113, 322, 574, 1122
CpoI	CGGWCCG	1 cut(s)	322
CseI	GACGC	1 cut(s)	176
Csp6I	GTAC	2 cut(s)	902, 912
CspI	CGGWCCG	1 cut(s)	322
CviAII	CATG	3 cut(s)	39, 398, 562
CviQI	GTAC	2 cut(s)	902, 912
DdeI	CTNAG	2 cut(s)	666, 898
DpnI	GATC	4 cut(s)	156, 318, 795, 1101
DpnII	GATC	4 cut(s)	154, 316, 793, 1099
DraIII	CACNNNGTG	1 cut(s)	109
Eam1104I	CTCTTC	1 cut(s)	243
EarI	CTCTTC	1 cut(s)	243
EciI	GGCGGA	2 cut(s)	105, 220
Eco31I	GGTCTC	2 cut(s)	486, 938
Eco47I	GGWCC	2 cut(s)	113, 322
Eco57I	CTGAAG	1 cut(s)	395
EcoRI	GAATTC	1 cut(s)	332
EcoRII	CCWGG	2 cut(s)	179, 789
FaeI	CATG	3 cut(s)	42, 401, 565
FaqI	GGGAC	2 cut(s)	227, 714
FatI	CATG	3 cut(s)	38, 397, 561
FauI	CCCGC	1 cut(s)	913
FauNDI	CATATG	2 cut(s)	298, 950
FbaI	TGATCA	1 cut(s)	1099
FblI	GTMKAC	1 cut(s)	1032
FokI	GGATG	1 cut(s)	17
FspBI	CTAG	3 cut(s)	353, 726, 759
GlaI	GCGC	1 cut(s)	756
GsuI	CTGGAG	2 cut(s)	202, 256
HaeII	RGCGCY	1 cut(s)	758
HaeIII	GGCC	2 cut(s)	576, 1123
HapII	CCGG	5 cut(s)	320, 426, 577, 992, 1120
HgaI	GACGC	1 cut(s)	176
HhaI	GCGC	1 cut(s)	757
Hin1I	GRCGYC	1 cut(s)	168
Hin1II	CATG	3 cut(s)	42, 401, 565
Hin6I	GCGC	1 cut(s)	755
HinP1I	GCGC	1 cut(s)	755
HincII	GTYRAC	1 cut(s)	1033
HindII	GTYRAC	1 cut(s)	1033
HindIII	AAGCTT	1 cut(s)	73
HinfI	GANTC	4 cut(s)	742, 762, 932, 1022
HpaII	CCGG	5 cut(s)	320, 426, 577, 992, 1120
HphI	GGTGA	2 cut(s)	28, 1088
Hpy166II	GTNNAC	3 cut(s)	775, 890, 1033
Hpy188I	TCNGA	2 cut(s)	308, 1027
Hpy188III	TCNNGA	3 cut(s)	130, 320, 737
Hpy8I	GTNNAC	3 cut(s)	775, 890, 1033
Hpy99I	CGWCG	4 cut(s)	50, 67, 770, 1037
HpyAV	CCTTC	1 cut(s)	168
HpyCH4III	ACNGT	4 cut(s)	46, 263, 698, 887
HpyCH4IV	ACGT	3 cut(s)	137, 768, 1035
HpyF10VI	GCNNNNNNNGC	5 cut(s)	48, 192, 412, 754, 1064
HpyF3I	CTNAG	2 cut(s)	666, 898
HpySE526I	ACGT	3 cut(s)	137, 768, 1035
Hsp92I	GRCGYC	1 cut(s)	168
Hsp92II	CATG	3 cut(s)	42, 401, 565
HspAI	GCGC	1 cut(s)	755
Kpn2I	TCCGGA	1 cut(s)	319
Ksp22I	TGATCA	1 cut(s)	1099
Kzo9I	GATC	4 cut(s)	154, 316, 793, 1099
LmnI	GCTCC	4 cut(s)	183, 434, 553, 958
Lsp1109I	GCAGC	8 cut(s)	87, 97, 544, 616, 709, 835, 991, 994
MaeI	CTAG	3 cut(s)	353, 726, 759
MaeII	ACGT	3 cut(s)	137, 768, 1035
MaeIII	GTNAC	2 cut(s)	34, 263
MalI	GATC	4 cut(s)	156, 318, 795, 1101
MboI	GATC	4 cut(s)	154, 316, 793, 1099
MboII	GAAGA	6 cut(s)	51, 145, 230, 233, 388, 682
MflI	RGATCY	2 cut(s)	154, 793
MhlI	GDGCHC	1 cut(s)	691
MluCI	AATT	3 cut(s)	332, 446, 544
MroI	TCCGGA	1 cut(s)	319
MroXI	GAANNNNTTC	1 cut(s)	836
MseI	TTAA	1 cut(s)	384
MslI	CAYNNNNRTG	2 cut(s)	506, 566
MspI	CCGG	5 cut(s)	320, 426, 577, 992, 1120
MspR9I	CCNGG	3 cut(s)	181, 791, 992
Mva1269I	GAATGC	1 cut(s)	679
MvaI	CCWGG	2 cut(s)	181, 791
MwoI	GCNNNNNNNGC	5 cut(s)	48, 192, 412, 754, 1064
NciI	CCSGG	1 cut(s)	992
NdeI	CATATG	2 cut(s)	298, 950
NdeII	GATC	4 cut(s)	154, 316, 793, 1099
NlaIII	CATG	3 cut(s)	42, 401, 565
NlaIV	GGNNCC	3 cut(s)	114, 156, 819
NmuCI	GTSAC	1 cut(s)	34
NspI	RCATGY	2 cut(s)	42, 401
PciI	ACATGT	1 cut(s)	397
PcsI	WCGNNNNNNNCGW	1 cut(s)	62
PctI	GAATGC	1 cut(s)	679
PdmI	GAANNNNTTC	1 cut(s)	836
PfeI	GAWTC	4 cut(s)	742, 762, 932, 1022
PflMI	CCANNNNNTGG	1 cut(s)	796
PfoI	TCCNGGA	1 cut(s)	179
PscI	ACATGT	1 cut(s)	397
Psp6I	CCWGG	2 cut(s)	179, 789
PspGI	CCWGG	2 cut(s)	179, 789
PspN4I	GGNNCC	3 cut(s)	114, 156, 819
PspPI	GGNCC	4 cut(s)	113, 322, 574, 1122
PstI	CTGCAG	1 cut(s)	1069
PstNI	CAGNNNCTG	2 cut(s)	796, 1064
PsuI	RGATCY	2 cut(s)	154, 793
RsaI	GTAC	2 cut(s)	903, 913
RsaNI	GTAC	2 cut(s)	902, 912
RseI	CAYNNNNRTG	2 cut(s)	506, 566
Rsr2I	CGGWCCG	1 cut(s)	322
RsrII	CGGWCCG	1 cut(s)	322
SalI	GTCGAC	1 cut(s)	1031
SaqAI	TTAA	1 cut(s)	384
Sau3AI	GATC	4 cut(s)	154, 316, 793, 1099
Sau96I	GGNCC	4 cut(s)	113, 322, 574, 1122
ScaI	AGTACT	1 cut(s)	913
ScrFI	CCNGG	3 cut(s)	181, 791, 992
SduI	GDGCHC	1 cut(s)	691
SfcI	CTRYAG	1 cut(s)	1065
SinI	GGWCC	2 cut(s)	113, 322
SmiMI	CAYNNNNRTG	2 cut(s)	506, 566
SmlI	CTYRAG	2 cut(s)	70, 737
SmoI	CTYRAG	2 cut(s)	70, 737
Sse9I	AATT	3 cut(s)	332, 446, 544
SsiI	CCGC	7 cut(s)	83, 116, 119, 231, 712, 906, 1010
SspMI	CTAG	3 cut(s)	353, 726, 759
StyD4I	CCNGG	3 cut(s)	179, 789, 990
TaaI	ACNGT	4 cut(s)	46, 263, 698, 887
TaiI	ACGT	3 cut(s)	140, 771, 1038
TaqI	TCGA	4 cut(s)	247, 765, 866, 1032
TaqII	GACCGA	2 cut(s)	339, 510
TasI	AATT	3 cut(s)	332, 446, 544
TatI	WGTACW	1 cut(s)	911
TauI	GCSGC	2 cut(s)	85, 121
TfiI	GAWTC	4 cut(s)	742, 762, 932, 1022
Tru1I	TTAA	1 cut(s)	384
Tru9I	TTAA	1 cut(s)	384
TscAI	CASTG	2 cut(s)	696, 877
TseFI	GTSAC	1 cut(s)	34
TseI	GCWGC	8 cut(s)	85, 100, 532, 604, 722, 848, 1004, 1007
Tsp45I	GTSAC	1 cut(s)	34
TspDTI	ATGAA	1 cut(s)	268
TspGWI	ACGGA	3 cut(s)	179, 527, 605
TspRI	CASTG	2 cut(s)	696, 877
Van91I	CCANNNNNTGG	1 cut(s)	796
VpaK11BI	GGWCC	2 cut(s)	113, 322
XapI	RAATTY	2 cut(s)	332, 544
XceI	RCATGY	2 cut(s)	42, 401
XmiI	GTMKAC	1 cut(s)	1032
XmnI	GAANNNNTTC	1 cut(s)	836
XspI	CTAG	3 cut(s)	353, 726, 759
ZrmI	AGTACT	1 cut(s)	913

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.