Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0007650

Physical Location & Seq

Genomic Coordinates Reverse (-)

4855707 .. 4867087

Gene Structure 11381 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1071100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2676 bp

ATGAGAGAGAACACGAGAGAGAACATGTCGAACCGAAACTCTCACCCCCGGCCAGCCGGAAGCAAGGGTTTCCCAAAACCCCAAAAGGTCTTCGTCCCCAAGAATCCAGACCACAACAATCCAATCCCTCGAGAGCCCAATCCCAACCCCACGCTCTCCACCTCACTCCGGCAATCGCTCTCCAAGCAGCAATCCAATCCCGAAACTTCCCCCGCTGCTTCGGCACCGCCGTCGGGGAGCAGGGTTCGGATGGGGGAGAAAGGAGAGTGGGTGCCGAGCAGAGCTCAGGGTGGCAATTTCGTAAGTTACTTACCTCAGGACGAGGCGGTGGCGGCTGGGCTGGGCGCCAACGAAGGTGGATTGGACGCCCTGGAGTCGCAAAGAGTGGTGGATCTTCTTAACAGAGAGCTCTCTCGCTTGCTGAAGTTAAACCCAAAGGAGTTTTGGCGACAAGTGGCTAGTGATGCATCGTTGCATGAATTTCTGGATAGCTTCCTACAGTTTAGGAGCAGGTGGTATGATTTCGCTCATCGCGGCGCCAAGGAATTGGTGGCGGGGGTCATTGTTGGGGAGTTCGAATTGAGCCGGCGTGTTTTTATGGTGTTATATCGAATATCTTCTAATAGGGATCCTGGCGCTCGTCTTGCTGATATTCTCAGTCCAAAAGATCATGAAGTTCTTTTGCAGGAGAAGAAGTTGCTGGGCTTGCCTACGTTGTTAGACATATGTGCTATATATGGCCATGAAAATGAAGATTTGACCAGAGTGCTGGTTGGCAATGCAGTGAAAGCTCATACTAGGATCCATGACAATTTGACTGCAGTTGCATCACATTTCCTTAGCATTGTCCAAACAATGTACCAACGTAGCAGCTCAGCCTTGGAGACCCTTTTTTCCCATGGAAACTCTGGAGTACATGGATCCAGCCGTCTTCTTGCTGATTTATTGGAAGTTATGGACTTCATTAACGATGCAATTGTATCCATGGATGCTTTCCTAACTGCATACGAACCATCCGCTGTCTTCTTTTTGTGCCCTGTTGAAACCAGTCATGGGAACGAGGGATTACTAAGTACTCTAGCAAGGTTGCATGATTCATTGCTTCCATCTTTGCAGCGTGGGTTTCGAATAATTTCAGAAGACGGAGAAGATAAAATGGTGTCTAATATTGCTTTCAGTTTGAAGATGTTATCATTGAGAATTGTCAGATTTGGTTGGAAACTATTGGATTTATGCTATCTAAGTGATGATGCCTTCAAAGATAACCTCCCCATTCCGGCTGCTGCGGAGATGTTTCCAGCCAAAGTGGAGGATCTTTTCATTAGAGCAGATATACTGGTTCAAACTTTAAGAGAGATTAATGGAATTTCCGTATGTGCTCAGGAGAACCAGAATAGGCAAACATTTCTTCAAAACGTTGAAAAGAACTTCAACATTATGAGCAAAATGGAGAATTTACAAAATACTGGGTGGATAATCATGGATGATGAGCAACTGGGCTACGTATATGTAATACTTACGTCTACTCAGAAACCCATAGTTACGCAGCAGCCCAGTACGAAAGCCGCTTTGACGAACAAAAAGGTGCAGATAGATGAAGATGTTGCAATTGTGGAGTCACGAATCAGTCAAATAAAGGACCTCTTCCCTGAATATGGAAAAGGTTTTTTAGCTGCTTGTCTTGAAGCCTATAACCAAAATCCTGAGGAGGTTATTCAGAGGATTCTTGAAGGAACTCTTCATGAAGATCTGCAGTCCTTGGATACGTCATTAGAGACGATGCCAACACCCAAGACTGCGACCGTTAGTAAGAACGATAAAGGGAAAGAAAAGCTAGTTGAGTTTACAGCACCACACGCCACTGGTACAGCAGCCGTTGTGAGGGATAAACAAAATGGTGGCCCTTTGGTTTCATCATCCTCATCACAAGGAAGGTTTGTTAGGAAGTCTAAAGCAGACATGCCTGACACTGATACACTGGATGACAGAAATGAGAAATACATTGCAAAAACTGCTGCTCTTATTTCACAGTTTGAGTATGACGATGAGTATGATGACTCCTTCGATGATCTGGGTCTGAGTGTTGCAGATTCAGGAGTGGGGGAAAGCGAGATATTTAGTCAAAAAAGCAGCTCAATTACGGGTGGGCCGTGGGAAACACAAACTGGAAGTTCATCCCAGGATGCAGCTAATTCCAAATGGGGCTCCAGAAAGAAGCCCCAATATTATGTCAAGGATGGTAAGAATTACAGTTACAAAGTTGCAGGTTCGGTTGCAGTTGCAAATGCTGGTGAAGCATCATTAATTACTGAAGCACAACAAGAAATGATTCATGGCCTTGGTCGTGGCGGCAATCTTCCTCTTGGCGCGGTTAAGAAGCTGACAGAATACTCGGAGGAGCAAGACAAACAATTTGACAATTCTCAGGCCGAAGAGAGGGGTCAGATGGATGGGAGAGGGAGAGGGTTTAGTGGGAATGCTAGGGGCCGAGGAAGGAGAGGTGAGGAGCAAGATAATAGACAAAACGAGAACGGTGACGGTGAAGACGGAGAGAATGCCGGAAATCAAAGGGGGAGGAGGGGAAGGAGAGGAGGAGGCAGAGGGGGAGGGGGAAGAAATAATTTCAGGAAGGACCGGGCCATGAATAAGCACTTTTCTGGTTTAGGTGGCTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006281 GO:0006304 GO:0006307 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034641 GO:0035510 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0099053 GO:1901360 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CUE

Protein Analysis

892

Amino Acids

98.32

Weight (kDa)

5.85

Isoelectric Point (pI)

39.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CUE	PF02845	542 - 579	3.4e-10	CUE domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007650)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G27752 AT1G27752
fragaria_vesca	FvH4_2g33210 FvH4_2g33210 FvH4_2g33210 FvH4_2g33210
malus_domestica	MD03G1160700.v1.1 MD08G1085900.v1.1 MD15G1071100.v1.1
prunus_persica	Prupe.1G423800_v2.0.a1 Prupe.1G423800_v2.0.a1 Prupe.1G423800_v2.0.a1 Prupe.1G423800_v2.0.a1
pyrus_communis	pycom08g07060 pycom15g06690
rosa_chinensis	RchiOBHm_Chr6g0309461
rosa_laevigata	RLG00000013072
rosa_multiflora	Rmu_sc0014034.1_g000003
rosa_rugosa	Rorug06G0372200
rosa_samantha	Rh6AG485300 Rh6BG494600 Rh6CG499600 Rh6DG485700
rosa_wichuraiana	Rw6G042270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	501
Acc36I	ACCTGC	2 cut(s)	501, 2255
AccB1I	GGYRCC	4 cut(s)	223, 271, 344, 536
AccI	GTMKAC	1 cut(s)	1523
AccII	CGCG	2 cut(s)	534, 2369
AclI	AACGTT	1 cut(s)	1416
AclWI	GGATC	8 cut(s)	399, 623, 636, 796, 809, 915, 928, 1320
AcoI	YGGCCR	2 cut(s)	50, 739
AcsI	RAATTY	3 cut(s)	479, 1365, 1453
AcuI	CTGAAG	2 cut(s)	443, 2331
AcyI	GRCGYC	3 cut(s)	345, 366, 537
AfaI	GTAC	5 cut(s)	860, 915, 1075, 1558, 1867
AfiI	CCNNNNNNNGG	7 cut(s)	168, 233, 1053, 1276, 1655, 1881, 2436
AflIII	ACRYGT	1 cut(s)	24
AgsI	TTSAA	9 cut(s)	1043, 1183, 1258, 1343, 1412, 1421, 1432, 1685, 1730
AjnI	CCWGG	3 cut(s)	369, 631, 2178
Alw21I	GWGCWC	3 cut(s)	286, 411, 1381
Alw26I	GTCTC	2 cut(s)	878, 1769
AlwI	GGATC	8 cut(s)	399, 623, 636, 796, 809, 915, 928, 1320
Ama87I	CYCGRG	1 cut(s)	129
AoxI	GGCC	8 cut(s)	50, 739, 1900, 2147, 2335, 2427, 2485, 2637
ApoI	RAATTY	3 cut(s)	479, 1365, 1453
ArsI	GACNNNNNNTTYG	2 cut(s)	2396, 2428
AseI	ATTAAT	2 cut(s)	1359, 2303
Asp700I	GAANNNNTTC	3 cut(s)	616, 1132, 2328
AspLEI	GCGC	4 cut(s)	347, 539, 638, 2369
AspS9I	GGNCC	6 cut(s)	1639, 1901, 2147, 2485, 2632, 2637
AsuC2I	CCSGG	2 cut(s)	49, 2636
AsuHPI	GGTGA	5 cut(s)	35, 2303, 2513, 2546, 2552
AsuII	TTCGAA	2 cut(s)	576, 1126
AvaI	CYCGRG	1 cut(s)	129
AvaII	GGWCC	2 cut(s)	1639, 2632
AxyI	CCTNAGG	2 cut(s)	315, 1704
BaeGI	GKGCMC	1 cut(s)	1037
BalI	TGGCCA	1 cut(s)	741
BamHI	GGATCC	3 cut(s)	628, 801, 920
BanI	GGYRCC	4 cut(s)	223, 271, 344, 536
BanII	GRGCYC	4 cut(s)	138, 286, 411, 2207
BauI	CACGAG	1 cut(s)	13
BbsI	GAAGAC	5 cut(s)	82, 923, 1015, 1146, 2550
Bbv12I	GWGCWC	3 cut(s)	286, 411, 1381
BccI	CCATC	6 cut(s)	244, 1021, 1114, 2231, 2440, 2444
BceAI	ACGGC	4 cut(s)	214, 912, 1859, 2134
BcgI	CGANNNNNNTGC	2 cut(s)	213, 247
BciT130I	CCWGG	3 cut(s)	371, 633, 2180
BciVI	GTATCC	2 cut(s)	991, 1756
BcnI	CCSGG	2 cut(s)	49, 2636
BcoDI	GTCTC	2 cut(s)	878, 1769
BfaI	CTAG	6 cut(s)	459, 798, 1079, 1835, 2481, 2674
BfmI	CTRYAG	3 cut(s)	497, 819, 1751
BfoI	RGCGCY	3 cut(s)	348, 540, 639
BfuAI	ACCTGC	2 cut(s)	501, 2255
BfuI	GTATCC	2 cut(s)	991, 1756
BglII	AGATCT	1 cut(s)	1747
BlpI	GCTNAGC	1 cut(s)	874
BmcAI	AGTACT	1 cut(s)	1075
Bme1390I	CCNGG	5 cut(s)	49, 371, 633, 2180, 2636
Bme18I	GGWCC	2 cut(s)	1639, 2632
BmeT110I	CYCGRG	1 cut(s)	129
BmgT120I	GGNCC	6 cut(s)	1639, 1901, 2147, 2485, 2632, 2637
BmiI	GGNNCC	9 cut(s)	225, 273, 346, 538, 630, 803, 922, 2206, 2486
BmrFI	CCNGG	5 cut(s)	49, 371, 633, 2180, 2636
BmrI	ACTGGG	3 cut(s)	1476, 1505, 1548
BmsI	GCATC	9 cut(s)	454, 476, 836, 961, 979, 1240, 1770, 2173, 2306
BmuI	ACTGGG	3 cut(s)	1476, 1505, 1548
BpiI	GAAGAC	5 cut(s)	82, 923, 1015, 1146, 2550
BplI	GAGNNNNNCTC	2 cut(s)	268, 300
BpmI	CTGGAG	3 cut(s)	392, 930, 2191
Bpu10I	CCTNAGC	3 cut(s)	285, 839, 1380
Bpu1102I	GCTNAGC	1 cut(s)	874
Bpu14I	TTCGAA	2 cut(s)	576, 1126
BpuMI	CCSGG	2 cut(s)	49, 2636
BsaAI	YACGTR	1 cut(s)	1504
BsaHI	GRCGYC	3 cut(s)	345, 366, 537
BsaI	GGTCTC	1 cut(s)	878
BsaXI	ACNNNNNCTCC	4 cut(s)	255, 285, 680, 710
Bsc4I	CCNNNNNNNGG	7 cut(s)	168, 233, 1053, 1276, 1655, 1881, 2436
Bse118I	RCCGGY	1 cut(s)	585
Bse1I	ACTGG	8 cut(s)	1047, 1341, 1471, 1500, 1554, 1867, 1983, 2170
Bse21I	CCTNAGG	2 cut(s)	315, 1704
Bse3DI	GCAATG	3 cut(s)	784, 1097, 2001
BseBI	CCWGG	3 cut(s)	371, 633, 2180
BseLI	CCNNNNNNNGG	7 cut(s)	168, 233, 1053, 1276, 1655, 1881, 2436
BseMI	GCAATG	3 cut(s)	784, 1097, 2001
BseMII	CTCAG	9 cut(s)	299, 329, 670, 888, 1394, 1541, 1695, 2069, 2438
BseNI	ACTGG	8 cut(s)	1047, 1341, 1471, 1500, 1554, 1867, 1983, 2170
BseRI	GAGGAG	6 cut(s)	1721, 2411, 2519, 2590, 2604, 2607
BseSI	GKGCMC	1 cut(s)	1037
BseYI	CCCAGC	3 cut(s)	335, 340, 700
BsgI	GTGCAG	1 cut(s)	1607
Bsh1236I	CGCG	2 cut(s)	534, 2369
Bsh1285I	CGRYCG	1 cut(s)	1803
BshFI	GGCC	8 cut(s)	52, 741, 1902, 2149, 2337, 2429, 2487, 2639
BshNI	GGYRCC	4 cut(s)	223, 271, 344, 536
BsiEI	CGRYCG	1 cut(s)	1803
BsiHKAI	GWGCWC	3 cut(s)	286, 411, 1381
BsiHKCI	CYCGRG	1 cut(s)	129
BsiSI	CCGG	7 cut(s)	49, 57, 169, 586, 1277, 2559, 2635
BslFI	GGGAC	1 cut(s)	80
BslI	CCNNNNNNNGG	7 cut(s)	168, 233, 1053, 1276, 1655, 1881, 2436
BsmAI	GTCTC	2 cut(s)	878, 1769
BsmBI	CGTCTC	1 cut(s)	1769
BsmFI	GGGAC	1 cut(s)	80
BsmI	GAATGC	2 cut(s)	2482, 2560
BsnI	GGCC	8 cut(s)	52, 741, 1902, 2149, 2337, 2429, 2487, 2639
Bso31I	GGTCTC	1 cut(s)	878
BsoBI	CYCGRG	1 cut(s)	129
Bsp119I	TTCGAA	2 cut(s)	576, 1126
Bsp1286I	GDGCHC	6 cut(s)	138, 286, 411, 1037, 1381, 2207
Bsp143I	GATC	8 cut(s)	391, 628, 667, 801, 920, 1312, 1747, 2068
Bsp1720I	GCTNAGC	1 cut(s)	874
Bsp19I	CCATGG	2 cut(s)	898, 984
BspANI	GGCC	8 cut(s)	52, 741, 1902, 2149, 2337, 2429, 2487, 2639
BspCNI	CTCAG	9 cut(s)	298, 328, 669, 887, 1393, 1540, 1696, 2070, 2437
BspFNI	CGCG	2 cut(s)	534, 2369
BspHI	TCATGA	2 cut(s)	670, 1741
BspLI	GGNNCC	9 cut(s)	225, 273, 346, 538, 630, 803, 922, 2206, 2486
BspMAI	CTGCAG	2 cut(s)	823, 1755
BspMI	ACCTGC	2 cut(s)	501, 2255
BspPI	GGATC	8 cut(s)	399, 623, 636, 796, 809, 915, 928, 1320
BspT104I	TTCGAA	2 cut(s)	576, 1126
BspT107I	GGYRCC	4 cut(s)	223, 271, 344, 536
BspTNI	GGTCTC	1 cut(s)	878
BsrDI	GCAATG	3 cut(s)	784, 1097, 2001
BsrFI	RCCGGY	1 cut(s)	585
BsrI	ACTGG	8 cut(s)	1047, 1341, 1471, 1500, 1554, 1867, 1983, 2170
BssAI	RCCGGY	1 cut(s)	585
BssMI	GATC	8 cut(s)	391, 628, 667, 801, 920, 1312, 1747, 2068
BssNI	GRCGYC	3 cut(s)	345, 366, 537
BssSI	CACGAG	1 cut(s)	13
BssT1I	CCWWGG	6 cut(s)	540, 879, 898, 984, 1758, 2338
Bst2BI	CACGAG	1 cut(s)	13
Bst2UI	CCWGG	3 cut(s)	371, 633, 2180
Bst4CI	ACNGT	6 cut(s)	501, 1804, 2031, 2252, 2534, 2540
Bst6I	CTCTTC	3 cut(s)	1649, 1743, 2427
BstACI	GRCGYC	3 cut(s)	345, 366, 537
BstAPI	GCANNNNNTGC	1 cut(s)	2012
BstBAI	YACGTR	1 cut(s)	1504
BstBI	TTCGAA	2 cut(s)	576, 1126
BstC8I	GCNNGC	4 cut(s)	54, 419, 587, 707
BstDSI	CCRYGG	3 cut(s)	898, 984, 2150
BstFNI	CGCG	2 cut(s)	534, 2369
BstH2I	RGCGCY	3 cut(s)	348, 540, 639
BstHHI	GCGC	4 cut(s)	347, 539, 638, 2369
BstKTI	GATC	8 cut(s)	394, 631, 670, 804, 923, 1315, 1750, 2071
BstMAI	GTCTC	2 cut(s)	878, 1769
BstMBI	GATC	8 cut(s)	391, 628, 667, 801, 920, 1312, 1747, 2068
BstMCI	CGRYCG	1 cut(s)	1803
BstNI	CCWGG	3 cut(s)	371, 633, 2180
BstNSI	RCATGY	2 cut(s)	28, 1963
BstSCI	CCNGG	5 cut(s)	47, 369, 631, 2178, 2634
BstSFI	CTRYAG	3 cut(s)	497, 819, 1751
BstSLI	GKGCMC	1 cut(s)	1037
BstSNI	TACGTA	1 cut(s)	1504
BstUI	CGCG	2 cut(s)	534, 2369
BstV2I	GAAGAC	5 cut(s)	82, 923, 1015, 1146, 2550
BstX2I	RGATCY	6 cut(s)	391, 628, 801, 920, 1312, 1747
BstXI	CCANNNNNNTGG	2 cut(s)	547, 769
BstYI	RGATCY	6 cut(s)	391, 628, 801, 920, 1312, 1747
Bsu36I	CCTNAGG	2 cut(s)	315, 1704
BsuI	GTATCC	2 cut(s)	991, 1756
BsuRI	GGCC	8 cut(s)	52, 741, 1902, 2149, 2337, 2429, 2487, 2639
BtgI	CCRYGG	3 cut(s)	898, 984, 2150
BtgZI	GCGATG	1 cut(s)	515
BtsI	GCAGTG	1 cut(s)	789
BtsIMutI	CAGTG	4 cut(s)	789, 1860, 1968, 1976
BveI	ACCTGC	2 cut(s)	501, 2255
Cac8I	GCNNGC	4 cut(s)	54, 419, 587, 707
CciI	TCATGA	2 cut(s)	670, 1741
CfoI	GCGC	4 cut(s)	347, 539, 638, 2369
Cfr10I	RCCGGY	1 cut(s)	585
Cfr13I	GGNCC	6 cut(s)	1639, 1901, 2147, 2485, 2632, 2637
CseI	GACGC	1 cut(s)	374
Csp6I	GTAC	5 cut(s)	859, 914, 1074, 1557, 1866
CspCI	CAANNNNNGTGG	2 cut(s)	337, 372
CviQI	GTAC	5 cut(s)	859, 914, 1074, 1557, 1866
DinI	GGCGCC	2 cut(s)	346, 538
DpnI	GATC	8 cut(s)	393, 630, 669, 803, 922, 1314, 1749, 2070
DpnII	GATC	8 cut(s)	391, 628, 667, 801, 920, 1312, 1747, 2068
EaeI	YGGCCR	2 cut(s)	50, 739
Eam1104I	CTCTTC	3 cut(s)	1649, 1743, 2427
EarI	CTCTTC	3 cut(s)	1649, 1743, 2427
Ecl136II	GAGCTC	2 cut(s)	284, 409
Eco105I	TACGTA	1 cut(s)	1504
Eco130I	CCWWGG	6 cut(s)	540, 879, 898, 984, 1758, 2338
Eco24I	GRGCYC	4 cut(s)	138, 286, 411, 2207
Eco31I	GGTCTC	1 cut(s)	878
Eco47I	GGWCC	2 cut(s)	1639, 2632
Eco53kI	GAGCTC	2 cut(s)	284, 409
Eco57I	CTGAAG	2 cut(s)	443, 2331
Eco81I	CCTNAGG	2 cut(s)	315, 1704
Eco88I	CYCGRG	1 cut(s)	129
EcoICRI	GAGCTC	2 cut(s)	284, 409
EcoO109I	RGGNCCY	1 cut(s)	1639
EcoRII	CCWGG	3 cut(s)	369, 631, 2178
EcoT14I	CCWWGG	6 cut(s)	540, 879, 898, 984, 1758, 2338
EcoT22I	ATGCAT	1 cut(s)	469
EcoT38I	GRGCYC	4 cut(s)	138, 286, 411, 2207
EgeI	GGCGCC	2 cut(s)	346, 538
EheI	GGCGCC	2 cut(s)	346, 538
ErhI	CCWWGG	6 cut(s)	540, 879, 898, 984, 1758, 2338
Esp3I	CGTCTC	1 cut(s)	1769
FalI	AAGNNNNNCTT	4 cut(s)	1628, 1660, 1722, 1754
FaqI	GGGAC	1 cut(s)	80
FauI	CCCGC	2 cut(s)	220, 547
FauNDI	CATATG	1 cut(s)	725
FblI	GTMKAC	1 cut(s)	1523
FriOI	GRGCYC	4 cut(s)	138, 286, 411, 2207
FspBI	CTAG	6 cut(s)	459, 798, 1079, 1835, 2481, 2674
GlaI	GCGC	4 cut(s)	346, 538, 637, 2368
GsaI	CCCAGC	3 cut(s)	339, 344, 704
GsuI	CTGGAG	3 cut(s)	392, 930, 2191
HaeII	RGCGCY	3 cut(s)	348, 540, 639
HaeIII	GGCC	8 cut(s)	52, 741, 1902, 2149, 2337, 2429, 2487, 2639
HapII	CCGG	7 cut(s)	49, 57, 169, 586, 1277, 2559, 2635
HgaI	GACGC	1 cut(s)	374
HhaI	GCGC	4 cut(s)	347, 539, 638, 2369
Hin1I	GRCGYC	3 cut(s)	345, 366, 537
Hin6I	GCGC	4 cut(s)	345, 537, 636, 2367
HinP1I	GCGC	4 cut(s)	345, 537, 636, 2367
HinfI	GANTC	9 cut(s)	103, 374, 1094, 1616, 1623, 1723, 2057, 2090, 2329
HpaII	CCGG	7 cut(s)	49, 57, 169, 586, 1277, 2559, 2635
HphI	GGTGA	5 cut(s)	35, 2303, 2513, 2546, 2552
Hpy166II	GTNNAC	2 cut(s)	1524, 1845
Hpy188I	TCNGA	8 cut(s)	249, 1138, 1208, 1530, 1719, 2079, 2395, 2445
Hpy8I	GTNNAC	2 cut(s)	1524, 1845
Hpy99I	CGWCG	1 cut(s)	235
HpyAV	CCTTC	8 cut(s)	347, 1264, 1724, 1926, 2071, 2487, 2577, 2623
HpyCH4III	ACNGT	6 cut(s)	501, 1804, 2031, 2252, 2534, 2540
HpyCH4IV	ACGT	6 cut(s)	713, 865, 1416, 1503, 1520, 1766
HpySE526I	ACGT	6 cut(s)	713, 865, 1416, 1503, 1520, 1766
Hsp92I	GRCGYC	3 cut(s)	345, 366, 537
HspAI	GCGC	4 cut(s)	345, 537, 636, 2367
KasI	GGCGCC	2 cut(s)	344, 536
KroI	GCCGGC	1 cut(s)	585
KroNI	GCCGGC	1 cut(s)	587
Kzo9I	GATC	8 cut(s)	391, 628, 667, 801, 920, 1312, 1747, 2068
LmnI	GCTCC	5 cut(s)	237, 507, 2210, 2398, 2506
LweI	GCATC	9 cut(s)	454, 476, 836, 961, 979, 1240, 1770, 2173, 2306
MaeI	CTAG	6 cut(s)	459, 798, 1079, 1835, 2481, 2674
MaeII	ACGT	6 cut(s)	713, 865, 1416, 1503, 1520, 1766
MaeIII	GTNAC	5 cut(s)	305, 1540, 1617, 2252, 2534
MalI	GATC	8 cut(s)	393, 630, 669, 803, 922, 1314, 1749, 2070
MboI	GATC	8 cut(s)	391, 628, 667, 801, 920, 1312, 1747, 2068
MfeI	CAATTG	2 cut(s)	975, 1608
MflI	RGATCY	6 cut(s)	391, 628, 801, 920, 1312, 1747
MhlI	GDGCHC	6 cut(s)	138, 286, 411, 1037, 1381, 2207
MlsI	TGGCCA	1 cut(s)	741
MluNI	TGGCCA	1 cut(s)	741
Mly113I	GGCGCC	2 cut(s)	345, 537
MlyI	GAGTC	3 cut(s)	383, 1625, 2051
MmeI	TCCRAC	1 cut(s)	1196
Mox20I	TGGCCA	1 cut(s)	741
Mph1103I	ATGCAT	1 cut(s)	469
MroNI	GCCGGC	1 cut(s)	585
MroXI	GAANNNNTTC	3 cut(s)	616, 1132, 2328
MscI	TGGCCA	1 cut(s)	741
MseI	TTAA	7 cut(s)	399, 428, 966, 1349, 1359, 2303, 2373
MslI	CAYNNNNRTG	1 cut(s)	747
Msp20I	TGGCCA	1 cut(s)	741
MspA1I	CMGCKG	2 cut(s)	215, 1019
MspI	CCGG	7 cut(s)	49, 57, 169, 586, 1277, 2559, 2635
MspR9I	CCNGG	5 cut(s)	49, 371, 633, 2180, 2636
MunI	CAATTG	2 cut(s)	975, 1608
Mva1269I	GAATGC	2 cut(s)	2482, 2560
MvaI	CCWGG	3 cut(s)	371, 633, 2180
MvnI	CGCG	2 cut(s)	534, 2369
NaeI	GCCGGC	1 cut(s)	587
NarI	GGCGCC	2 cut(s)	345, 537
NciI	CCSGG	2 cut(s)	49, 2636
NcoI	CCATGG	2 cut(s)	898, 984
NdeI	CATATG	1 cut(s)	725
NdeII	GATC	8 cut(s)	391, 628, 667, 801, 920, 1312, 1747, 2068
NgoMIV	GCCGGC	1 cut(s)	585
NlaIV	GGNNCC	9 cut(s)	225, 273, 346, 538, 630, 803, 922, 2206, 2486
NmeAIII	GCCGAG	2 cut(s)	300, 2513
NmuCI	GTSAC	2 cut(s)	1617, 2534
NsiI	ATGCAT	1 cut(s)	469
NspI	RCATGY	2 cut(s)	28, 1963
NspV	TTCGAA	2 cut(s)	576, 1126
PaeR7I	CTCGAG	1 cut(s)	129
PagI	TCATGA	2 cut(s)	670, 1741
PaqCI	CACCTGC	1 cut(s)	501
PciI	ACATGT	1 cut(s)	24
PcsI	WCGNNNNNNNCGW	2 cut(s)	227, 2147
PctI	GAATGC	2 cut(s)	2482, 2560
PdiI	GCCGGC	1 cut(s)	587
PdmI	GAANNNNTTC	3 cut(s)	616, 1132, 2328
PfeI	GAWTC	6 cut(s)	103, 1094, 1623, 1723, 2090, 2329
PleI	GAGTC	3 cut(s)	382, 1624, 2051
PluTI	GGCGCC	2 cut(s)	348, 540
PpsI	GAGTC	3 cut(s)	382, 1624, 2051
Ppu21I	YACGTR	1 cut(s)	1504
PpuMI	RGGWCCY	1 cut(s)	1639
PscI	ACATGT	1 cut(s)	24
PshBI	ATTAAT	2 cut(s)	1359, 2303
Psp124BI	GAGCTC	2 cut(s)	286, 411
Psp1406I	AACGTT	1 cut(s)	1416
Psp5II	RGGWCCY	1 cut(s)	1639
Psp6I	CCWGG	3 cut(s)	369, 631, 2178
PspFI	CCCAGC	3 cut(s)	335, 340, 700
PspGI	CCWGG	3 cut(s)	369, 631, 2178
PspN4I	GGNNCC	9 cut(s)	225, 273, 346, 538, 630, 803, 922, 2206, 2486
PspPI	GGNCC	6 cut(s)	1639, 1901, 2147, 2485, 2632, 2637
PspPPI	RGGWCCY	1 cut(s)	1639
PstI	CTGCAG	2 cut(s)	823, 1755
PsuI	RGATCY	6 cut(s)	391, 628, 801, 920, 1312, 1747
RsaI	GTAC	5 cut(s)	860, 915, 1075, 1558, 1867
RsaNI	GTAC	5 cut(s)	859, 914, 1074, 1557, 1866
RseI	CAYNNNNRTG	1 cut(s)	747
SacI	GAGCTC	2 cut(s)	286, 411
SaqAI	TTAA	7 cut(s)	399, 428, 966, 1349, 1359, 2303, 2373
Sau3AI	GATC	8 cut(s)	391, 628, 667, 801, 920, 1312, 1747, 2068
Sau96I	GGNCC	6 cut(s)	1639, 1901, 2147, 2485, 2632, 2637
ScaI	AGTACT	1 cut(s)	1075
SchI	GAGTC	3 cut(s)	383, 1625, 2051
ScrFI	CCNGG	5 cut(s)	49, 371, 633, 2180, 2636
SduI	GDGCHC	6 cut(s)	138, 286, 411, 1037, 1381, 2207
SfaNI	GCATC	9 cut(s)	454, 476, 836, 961, 979, 1240, 1770, 2173, 2306
SfcI	CTRYAG	3 cut(s)	497, 819, 1751
SfoI	GGCGCC	2 cut(s)	346, 538
Sfr274I	CTCGAG	1 cut(s)	129
SfuI	TTCGAA	2 cut(s)	576, 1126
SinI	GGWCC	2 cut(s)	1639, 2632
SlaI	CTCGAG	1 cut(s)	129
SmiMI	CAYNNNNRTG	1 cut(s)	747
SmlI	CTYRAG	1 cut(s)	129
SmoI	CTYRAG	1 cut(s)	129
SnaBI	TACGTA	1 cut(s)	1504
SspDI	GGCGCC	2 cut(s)	344, 536
SspI	AATATT	2 cut(s)	1168, 2225
SspMI	CTAG	6 cut(s)	459, 798, 1079, 1835, 2481, 2674
SstI	GAGCTC	2 cut(s)	286, 411
StyD4I	CCNGG	5 cut(s)	47, 369, 631, 2178, 2634
StyI	CCWWGG	6 cut(s)	540, 879, 898, 984, 1758, 2338
TaaI	ACNGT	6 cut(s)	501, 1804, 2031, 2252, 2534, 2540
TaiI	ACGT	6 cut(s)	716, 868, 1419, 1506, 1523, 1769
TaqI	TCGA	6 cut(s)	29, 130, 576, 610, 1126, 2064
TatI	WGTACW	2 cut(s)	913, 1073
TauI	GCSGC	4 cut(s)	335, 537, 1568, 2352
TfiI	GAWTC	6 cut(s)	103, 1094, 1623, 1723, 2090, 2329
Tru1I	TTAA	7 cut(s)	399, 428, 966, 1349, 1359, 2303, 2373
Tru9I	TTAA	7 cut(s)	399, 428, 966, 1349, 1359, 2303, 2373
TscAI	CASTG	4 cut(s)	789, 1867, 1975, 1983
TseFI	GTSAC	2 cut(s)	1617, 2534
Tsp45I	GTSAC	2 cut(s)	1617, 2534
TspGWI	ACGGA	3 cut(s)	1158, 1360, 2562
TspRI	CASTG	4 cut(s)	789, 1867, 1975, 1983
VpaK11BI	GGWCC	2 cut(s)	1639, 2632
VspI	ATTAAT	2 cut(s)	1359, 2303
XapI	RAATTY	3 cut(s)	479, 1365, 1453
XceI	RCATGY	2 cut(s)	28, 1963
XcmI	CCANNNNNNNNNTGG	3 cut(s)	441, 547, 905
XhoI	CTCGAG	1 cut(s)	129
XmiI	GTMKAC	1 cut(s)	1523
XmnI	GAANNNNTTC	3 cut(s)	616, 1132, 2328
XspI	CTAG	6 cut(s)	459, 798, 1079, 1835, 2481, 2674
ZrmI	AGTACT	1 cut(s)	1075
Zsp2I	ATGCAT	1 cut(s)	469

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.