Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0009824

Physical Location & Seq

Genomic Coordinates Forward (+)

5699404 .. 5701572

Gene Structure 2169 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1083200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2169 bp

ATGAACTTGGCCTGTTCTATCGGCCTCTCTGCGAAGCCCAACCTTCCCTATTTCCACTATCTCCTAAAATCTCACAGCGAAAGCAAAAATCCTAAACAGGCACTGCTTCTTTTTCGCCAGTTGTTTGAGTACAATTTGAAGCCCAATGATCTCACTTTCTCTCTGCTCATTAAATCCTGCACTTCTTCCTCCTCCTCAGGTTTTAATTCAGCGAGCTCAAAACTAGAAGCAAATCAGATTCATACCCACTTGCTGAAATCTGGTGTCGATCGATATGTATATGTGAGCACTGCTCTTCTTGATTTGTATATGAAATTGGGTTGTGTAAAGGATGCCCAGAAGGTGTTTGATGATATGCCTGACAGAGATGTTGTCTCATGGAATGCGTTGATTTGTGGGTTTTCACGAAATGGGTATGATTTTGAAGCGTTGAGATGCTTTGTTCGAATGTGTAGAGAGGGGCTTTGTCCTTGTCCGACGACTTTGGTTAGTTTGGTTCCGTCTTGTGGTAGGCAAGAGTTAGTTTTTATTGGGAAATGTGTTCATGGGTTTGGAATTAAGGCGGGCCTGGATTTGGATTCTAACGTGAAGAATGTGCTTACCTCGATGTACGCTAAATCTGCAGATTTGGAGGCGGCGGAGCTCTTGTTTGAAGAGATAGTTGACAAGAGTGTAGTTTCTTGGAATACTATGATTGGCGCTTATGGACAAAATGGTTTCTTTCATGAGGCAATGCTTGTCTTTAAACGAATGCAGGAGGAAAATGTTCCGGCCAATCTGGTGACCATGGTAAGCCTCCTGTCAGCAAATGCAAATCCAGAGTCTACCCATTGTCATGCCCTTAAAACTGGTCATGCAAATGATGCTTCCGTGATTACTTCGCTAATTTGCGAATATGCAACACGAGGGAACACAGAATCCGGAAAACTGCTTTACAAGTCATTGCCTCAGAAGAACTTGGTTTCCCTGACTGCAATTATTTCAAGCTATGCTGAGAAAGATAACATAGCTCAGGTACTGGAATGCTTTGCTCACTTGCAGCGGTTAGACATGAAACTAGACGCAGTTTCTATGGTTTGCATCCTTCATGGGATAAAAGTCCCTGTTGAAATTGGCCTTGGACTTGCTTTCCATGGTTATGGAATAAAGATTGGGCTGACGGCTGATTCTTTGGTTGCAAATGGTTTGATAAGCATGTACTCGAGGTTTGACAAGATAGAAGCCGCTTTTTCTTTATTCCTTGAGATGCAAGCAAAATCGTTGCTTACTTGGAATTCTTTGATATCGGGATGTGTACAAGTGGGAAGGTCAAGTGATGCCCTGGACTTGTTTATCCAAATGAAAATGTCCGGATACAGTCCAGATAGCATCACTATTTCTAGCTTACTATCTGGGTGTGGCCAAATTGGGTACTTGCGATTTGGGGAGAAACTTCATAACTATGTCCTCCGAAACAATCTTGAAGTGGAAGATTTTGTTGGAACCGCTCTTATAGACATGTACACCAAGTGTGGAAGAATAGAGCTTGCTGAAAGGGTGTTTAAGAGCATCCAAGAGCCATGTTTGGCATCGTGGAACTCAATCCTCTTGGGTTATAGCCACTACGGGTCAGAACATAGAGCTCTTGCTTGCTTCTTGAAAATGCAAGAGCAGGGGATAAAGCCCGATAATATCACATTTTTGGGAGTTTTAGCTGCTTGTACTCATGGAGGTTTTGTTGAGGAGGGAAGAAAATACTTCCAAATCATGAGGGAAGAGTTCAATATAGCGCCACGCTTGCAACACTGCGCTTGCATGGTTGGTCTCTTTGGCCGTGTGGGCTTCTTCCAGGAGGCATTGGTTTTCATCAGAGATATGGAGATGGAGCCTGACTTCGCAGTGTGGGGAGCCTTGCTGAATGCTTGTTGCATCCACCAAGAGGTCAAGCTTGGGGAGTACATAGCCAAAAAGTTGTTTCTCTTGGACAGTAAAAGTGGCGGGTTCTTCGTGCTGATGTCAAATCTTTACGCCGTTAAAGGGATGTGGGACGATGTAGCTAGGGTGAGGGAGATGATGAGAGGGACCGGAGGTGATGGTTGTTCAGGAGTGAGTGTTATTGAGACGAATGAAAATTTGTGCTTGAATAAACCTAGTTTGAATACAAATTTTGGGCAGATTCTGTATATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0016070 GO:0016787 GO:0016788 GO:0031425 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1900865 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko04973 map00500 map01100 map04973

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

723

Amino Acids

79.93

Weight (kDa)

6.33

Isoelectric Point (pI)

36.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR	PF01535	125 - 155	6e-06	PPR repeat
PPR_2	PF13041	224 - 269	2.3e-09	PPR repeat family
PPR	PF01535	226 - 256	2.8e-09	PPR repeat
PPR_2	PF13041	422 - 466	5.5e-12	PPR repeat family
PPR	PF01535	423 - 451	2.3e-07	PPR repeat
PPR_2	PF13041	520 - 567	2.3e-06	PPR repeat family
E_motif	PF20431	638 - 700	4e-12	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009824)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G04860
fragaria_vesca	FvH4_2g34460
malus_domestica	MD15G1083200.v1.1
prunus_persica	Prupe.1G435700_v2.0.a1 Prupe.1G435700_v2.0.a1 Prupe.1G435700_v2.0.a1 Prupe.1G435700_v2.0.a1 Prupe.1G435700_v2.0.a1 Prupe.1G435700_v2.0.a1
pyrus_communis	pycom15g07770
rosa_chinensis	RchiOBHm_Chr6g0307911
rosa_laevigata	RLG00000010687
rosa_multiflora	Rmu_sc0003252.1_g000018 Rmu_sc0032565.1_g000001
rosa_roxburghii	Rroxscaffold_7G00160770
rosa_rugosa	Rorug06G0361600
rosa_samantha	Rh6AG470700 Rh6BG481700 Rh6CG487100 Rh6DG473200
rosa_wichuraiana	Rw6G041100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	1487
AccI	GTMKAC	1 cut(s)	824
AccIII	TCCGGA	2 cut(s)	920, 1349
AciI	CCGC	7 cut(s)	563, 635, 638, 1042, 1224, 1485, 1977
AcoI	YGGCCR	3 cut(s)	771, 1399, 1810
AcsI	RAATTY	3 cut(s)	1273, 2110, 2143
AdeI	CACNNNGTG	1 cut(s)	1509
AfaI	GTAC	9 cut(s)	131, 611, 1017, 1199, 1296, 1412, 1502, 1702, 1937
AfiI	CCNNNNNNNGG	4 cut(s)	506, 574, 1918, 2016
AflIII	ACRYGT	1 cut(s)	1497
AjnI	CCWGG	3 cut(s)	567, 1320, 1827
AjuI	GAANNNNNNNTTGG	4 cut(s)	1101, 1133, 1461, 1493
AloI	GAACNNNNNNTCC	4 cut(s)	902, 934, 947, 979
Alw21I	GWGCWC	4 cut(s)	218, 290, 645, 1624
Alw26I	GTCTC	3 cut(s)	379, 1808, 2093
AlwNI	CAGNNNCTG	3 cut(s)	103, 1018, 2158
Ama87I	CYCGRG	1 cut(s)	1201
Aor13HI	TCCGGA	2 cut(s)	920, 1349
AoxI	GGCC	7 cut(s)	9, 22, 565, 771, 1114, 1399, 1810
ApeKI	GCWGC	2 cut(s)	1039, 1694
ApoI	RAATTY	3 cut(s)	1273, 2110, 2143
ArsI	GACNNNNNNTTYG	2 cut(s)	806, 838
Asp700I	GAANNNNTTC	1 cut(s)	765
AspLEI	GCGC	3 cut(s)	701, 1771, 1790
AspS9I	GGNCC	2 cut(s)	565, 2061
AsuHPI	GGTGA	3 cut(s)	793, 2053, 2081
AsuII	TTCGAA	1 cut(s)	445
AvaI	CYCGRG	1 cut(s)	1201
AvaII	GGWCC	1 cut(s)	2061
AxyI	CCTNAGG	1 cut(s)	196
BalI	TGGCCA	1 cut(s)	1401
BanII	GRGCYC	3 cut(s)	218, 645, 1624
BauI	CACGAG	1 cut(s)	903
Bbv12I	GWGCWC	4 cut(s)	218, 290, 645, 1624
BbvI	GCAGC	2 cut(s)	1051, 1681
BccI	CCATC	2 cut(s)	1855, 2066
BceAI	ACGGC	3 cut(s)	1176, 1797, 1994
BciT130I	CCWGG	3 cut(s)	569, 1322, 1829
BciVI	GTATCC	1 cut(s)	1346
BcoDI	GTCTC	3 cut(s)	379, 1808, 2093
BfaI	CTAG	5 cut(s)	224, 1058, 1380, 2037, 2130
BfmI	CTRYAG	1 cut(s)	621
BfoI	RGCGCY	2 cut(s)	702, 1772
BfuI	GTATCC	1 cut(s)	1346
BglI	GCCNNNNNGGC	1 cut(s)	1818
BisI	GCNGC	4 cut(s)	636, 1040, 1224, 1695
BlsI	GCNGC	4 cut(s)	637, 1041, 1225, 1696
Bme1390I	CCNGG	3 cut(s)	569, 1322, 1829
Bme18I	GGWCC	1 cut(s)	2061
BmeT110I	CYCGRG	1 cut(s)	1201
BmgT120I	GGNCC	2 cut(s)	565, 2061
BmiI	GGNNCC	5 cut(s)	498, 1483, 1866, 1888, 2062
BmrFI	CCNGG	3 cut(s)	569, 1322, 1829
BplI	GAGNNNNNCTC	2 cut(s)	277, 309
Bpu10I	CCTNAGC	1 cut(s)	1011
Bpu14I	TTCGAA	1 cut(s)	445
BpuEI	CTTGAG	1 cut(s)	1262
Bsa29I	ATCGAT	1 cut(s)	271
BsaI	GGTCTC	1 cut(s)	1808
BsaJI	CCNNGG	4 cut(s)	786, 1117, 1132, 1320
BsaWI	WCCGGW	3 cut(s)	920, 1349, 2063
BsaXI	ACNNNNNCTCC	6 cut(s)	632, 662, 1701, 1731, 2076, 2106
Bsc4I	CCNNNNNNNGG	4 cut(s)	506, 574, 1918, 2016
Bse1I	ACTGG	3 cut(s)	118, 853, 1023
Bse21I	CCTNAGG	1 cut(s)	196
Bse3DI	GCAATG	2 cut(s)	738, 941
BseAI	TCCGGA	2 cut(s)	920, 1349
BseBI	CCWGG	3 cut(s)	569, 1322, 1829
BseCI	ATCGAT	1 cut(s)	271
BseDI	CCNNGG	4 cut(s)	786, 1117, 1132, 1320
BseGI	GGATG	6 cut(s)	337, 1080, 1295, 1548, 1908, 2025
BseLI	CCNNNNNNNGG	4 cut(s)	506, 574, 1918, 2016
BseMI	GCAATG	2 cut(s)	738, 941
BseMII	CTCAG	4 cut(s)	210, 962, 984, 1025
BseNI	ACTGG	3 cut(s)	118, 853, 1023
BseRI	GAGGAG	3 cut(s)	181, 184, 1736
BseXI	GCAGC	2 cut(s)	1051, 1681
BsgI	GTGCAG	1 cut(s)	163
Bsh1285I	CGRYCG	1 cut(s)	271
BshFI	GGCC	7 cut(s)	11, 24, 567, 773, 1116, 1401, 1812
BshVI	ATCGAT	1 cut(s)	271
BsiEI	CGRYCG	1 cut(s)	271
BsiHKAI	GWGCWC	4 cut(s)	218, 290, 645, 1624
BsiHKCI	CYCGRG	1 cut(s)	1201
BsiSI	CCGG	4 cut(s)	770, 921, 1350, 2064
BslFI	GGGAC	3 cut(s)	1085, 2039, 2074
BslI	CCNNNNNNNGG	4 cut(s)	506, 574, 1918, 2016
BsmAI	GTCTC	3 cut(s)	379, 1808, 2093
BsmBI	CGTCTC	1 cut(s)	2093
BsmFI	GGGAC	3 cut(s)	1085, 2039, 2074
BsmI	GAATGC	4 cut(s)	388, 756, 1028, 1903
BsnI	GGCC	7 cut(s)	11, 24, 567, 773, 1116, 1401, 1812
Bso31I	GGTCTC	1 cut(s)	1808
BsoBI	CYCGRG	1 cut(s)	1201
Bsp119I	TTCGAA	1 cut(s)	445
Bsp1286I	GDGCHC	4 cut(s)	218, 290, 645, 1624
Bsp13I	TCCGGA	2 cut(s)	920, 1349
Bsp1407I	TGTACA	2 cut(s)	1294, 1500
Bsp143I	GATC	2 cut(s)	148, 268
Bsp19I	CCATGG	2 cut(s)	786, 1132
BspACI	CCGC	7 cut(s)	563, 635, 638, 1042, 1224, 1485, 1977
BspANI	GGCC	7 cut(s)	11, 24, 567, 773, 1116, 1401, 1812
BspCNI	CTCAG	4 cut(s)	209, 961, 985, 1024
BspDI	ATCGAT	1 cut(s)	271
BspEI	TCCGGA	2 cut(s)	920, 1349
BspHI	TCATGA	2 cut(s)	724, 1746
BspLI	GGNNCC	5 cut(s)	498, 1483, 1866, 1888, 2062
BspMAI	CTGCAG	1 cut(s)	625
BspQI	GCTCTTC	1 cut(s)	300
BspT104I	TTCGAA	1 cut(s)	445
BspTNI	GGTCTC	1 cut(s)	1808
BsrBI	CCGCTC	1 cut(s)	1487
BsrDI	GCAATG	2 cut(s)	738, 941
BsrGI	TGTACA	2 cut(s)	1294, 1500
BsrI	ACTGG	3 cut(s)	118, 853, 1023
BssECI	CCNNGG	4 cut(s)	786, 1117, 1132, 1320
BssMI	GATC	2 cut(s)	148, 268
BssSI	CACGAG	1 cut(s)	903
BssT1I	CCWWGG	3 cut(s)	786, 1117, 1132
Bst2BI	CACGAG	1 cut(s)	903
Bst2UI	CCWGG	3 cut(s)	569, 1322, 1829
Bst4CI	ACNGT	2 cut(s)	1358, 1967
Bst6I	CTCTTC	3 cut(s)	300, 648, 1749
BstAPI	GCANNNNNTGC	1 cut(s)	863
BstAUI	TGTACA	2 cut(s)	1294, 1500
BstBI	TTCGAA	1 cut(s)	445
BstC8I	GCNNGC	7 cut(s)	214, 565, 1251, 1527, 1630, 1778, 1792
BstDEI	CTNAG	4 cut(s)	196, 948, 993, 1011
BstDSI	CCRYGG	2 cut(s)	786, 1132
BstEII	GGTNACC	1 cut(s)	781
BstF5I	GGATG	6 cut(s)	337, 1080, 1295, 1548, 1908, 2025
BstH2I	RGCGCY	2 cut(s)	702, 1772
BstHHI	GCGC	3 cut(s)	701, 1771, 1790
BstKTI	GATC	2 cut(s)	151, 271
BstMAI	GTCTC	3 cut(s)	379, 1808, 2093
BstMBI	GATC	2 cut(s)	148, 268
BstMCI	CGRYCG	1 cut(s)	271
BstMWI	GCNNNNNNNGC	4 cut(s)	620, 863, 1777, 1818
BstNI	CCWGG	3 cut(s)	569, 1322, 1829
BstNSI	RCATGY	2 cut(s)	1198, 1501
BstPI	GGTNACC	1 cut(s)	781
BstSCI	CCNGG	3 cut(s)	567, 1320, 1827
BstSFI	CTRYAG	1 cut(s)	621
BstV1I	GCAGC	2 cut(s)	1051, 1681
BstXI	CCANNNNNNTGG	1 cut(s)	1139
Bsu15I	ATCGAT	1 cut(s)	271
Bsu36I	CCTNAGG	1 cut(s)	196
BsuI	GTATCC	1 cut(s)	1346
BsuRI	GGCC	7 cut(s)	11, 24, 567, 773, 1116, 1401, 1812
BsuTUI	ATCGAT	1 cut(s)	271
BtgI	CCRYGG	2 cut(s)	786, 1132
BtsCI	GGATG	6 cut(s)	337, 1080, 1295, 1548, 1908, 2025
BtsI	GCAGTG	4 cut(s)	101, 288, 1783, 1884
BtsIMutI	CAGTG	4 cut(s)	101, 288, 1783, 1884
Cac8I	GCNNGC	7 cut(s)	214, 565, 1251, 1527, 1630, 1778, 1792
CaiI	CAGNNNCTG	3 cut(s)	103, 1018, 2158
CciI	TCATGA	2 cut(s)	724, 1746
CfoI	GCGC	3 cut(s)	701, 1771, 1790
Cfr13I	GGNCC	2 cut(s)	565, 2061
ClaI	ATCGAT	1 cut(s)	271
CseI	GACGC	1 cut(s)	1070
Csp6I	GTAC	9 cut(s)	130, 610, 1016, 1198, 1295, 1411, 1501, 1701, 1936
CviQI	GTAC	9 cut(s)	130, 610, 1016, 1198, 1295, 1411, 1501, 1701, 1936
DdeI	CTNAG	4 cut(s)	196, 948, 993, 1011
DpnI	GATC	2 cut(s)	150, 270
DpnII	GATC	2 cut(s)	148, 268
DraI	TTTAAA	1 cut(s)	745
DraIII	CACNNNGTG	1 cut(s)	1509
EaeI	YGGCCR	3 cut(s)	771, 1399, 1810
Eam1104I	CTCTTC	3 cut(s)	300, 648, 1749
EarI	CTCTTC	3 cut(s)	300, 648, 1749
EciI	GGCGGA	1 cut(s)	653
Ecl136II	GAGCTC	3 cut(s)	216, 643, 1622
Eco130I	CCWWGG	3 cut(s)	786, 1117, 1132
Eco24I	GRGCYC	3 cut(s)	218, 645, 1624
Eco31I	GGTCTC	1 cut(s)	1808
Eco32I	GATATC	1 cut(s)	1284
Eco47I	GGWCC	1 cut(s)	2061
Eco53kI	GAGCTC	3 cut(s)	216, 643, 1622
Eco81I	CCTNAGG	1 cut(s)	196
Eco88I	CYCGRG	1 cut(s)	1201
Eco91I	GGTNACC	1 cut(s)	781
EcoICRI	GAGCTC	3 cut(s)	216, 643, 1622
EcoO65I	GGTNACC	1 cut(s)	781
EcoRI	GAATTC	1 cut(s)	1273
EcoRII	CCWGG	3 cut(s)	567, 1320, 1827
EcoRV	GATATC	1 cut(s)	1284
EcoT14I	CCWWGG	3 cut(s)	786, 1117, 1132
EcoT38I	GRGCYC	3 cut(s)	218, 645, 1624
ErhI	CCWWGG	3 cut(s)	786, 1117, 1132
Esp3I	CGTCTC	1 cut(s)	2093
FaqI	GGGAC	3 cut(s)	1085, 2039, 2074
FauI	CCCGC	2 cut(s)	556, 1970
FblI	GTMKAC	1 cut(s)	824
Fnu4HI	GCNGC	4 cut(s)	636, 1040, 1224, 1695
FokI	GGATG	6 cut(s)	344, 1067, 1302, 1535, 1895, 2032
FriOI	GRGCYC	3 cut(s)	218, 645, 1624
Fsp4HI	GCNGC	4 cut(s)	636, 1040, 1224, 1695
FspBI	CTAG	5 cut(s)	224, 1058, 1380, 2037, 2130
GlaI	GCGC	3 cut(s)	700, 1770, 1789
GluI	GCNGC	4 cut(s)	636, 1040, 1224, 1695
HaeII	RGCGCY	2 cut(s)	702, 1772
HaeIII	GGCC	7 cut(s)	11, 24, 567, 773, 1116, 1401, 1812
HapII	CCGG	4 cut(s)	770, 921, 1350, 2064
HgaI	GACGC	1 cut(s)	1070
HhaI	GCGC	3 cut(s)	701, 1771, 1790
Hin6I	GCGC	3 cut(s)	699, 1769, 1788
HinP1I	GCGC	3 cut(s)	699, 1769, 1788
HincII	GTYRAC	1 cut(s)	664
HindII	GTYRAC	1 cut(s)	664
HindIII	AAGCTT	1 cut(s)	1925
HinfI	GANTC	6 cut(s)	238, 578, 821, 917, 1166, 2155
HpaII	CCGG	4 cut(s)	770, 921, 1350, 2064
HphI	GGTGA	3 cut(s)	793, 2053, 2081
Hpy166II	GTNNAC	4 cut(s)	664, 825, 1295, 1503
Hpy188I	TCNGA	6 cut(s)	237, 477, 951, 1451, 1612, 1850
Hpy8I	GTNNAC	4 cut(s)	664, 825, 1295, 1503
Hpy99I	CGWCG	1 cut(s)	481
HpyAV	CCTTC	4 cut(s)	53, 334, 1094, 1299
HpyCH4III	ACNGT	2 cut(s)	1358, 1967
HpyCH4IV	ACGT	1 cut(s)	585
HpyF10VI	GCNNNNNNNGC	4 cut(s)	620, 863, 1777, 1818
HpyF3I	CTNAG	4 cut(s)	196, 948, 993, 1011
HpySE526I	ACGT	1 cut(s)	585
HspAI	GCGC	3 cut(s)	699, 1769, 1788
Kpn2I	TCCGGA	2 cut(s)	920, 1349
Kzo9I	GATC	2 cut(s)	148, 268
LguI	GCTCTTC	1 cut(s)	300
LmnI	GCTCC	3 cut(s)	640, 1864, 1886
Lsp1109I	GCAGC	2 cut(s)	1051, 1681
MaeI	CTAG	5 cut(s)	224, 1058, 1380, 2037, 2130
MaeII	ACGT	1 cut(s)	585
MaeIII	GTNAC	1 cut(s)	781
MalI	GATC	2 cut(s)	150, 270
MbiI	CCGCTC	1 cut(s)	1487
MboI	GATC	2 cut(s)	148, 268
MhlI	GDGCHC	4 cut(s)	218, 290, 645, 1624
MlsI	TGGCCA	1 cut(s)	1401
MluNI	TGGCCA	1 cut(s)	1401
MlyI	GAGTC	1 cut(s)	830
MmeI	TCCRAC	2 cut(s)	500, 1459
Mox20I	TGGCCA	1 cut(s)	1401
MroI	TCCGGA	2 cut(s)	920, 1349
MroXI	GAANNNNTTC	1 cut(s)	765
MscI	TGGCCA	1 cut(s)	1401
MseI	TTAA	7 cut(s)	171, 204, 558, 744, 843, 1542, 2013
MslI	CAYNNNNRTG	4 cut(s)	834, 858, 1137, 1440
Msp20I	TGGCCA	1 cut(s)	1401
MspA1I	CMGCKG	1 cut(s)	1042
MspI	CCGG	4 cut(s)	770, 921, 1350, 2064
MspR9I	CCNGG	3 cut(s)	569, 1322, 1829
Mva1269I	GAATGC	4 cut(s)	388, 756, 1028, 1903
MvaI	CCWGG	3 cut(s)	569, 1322, 1829
MwoI	GCNNNNNNNGC	4 cut(s)	620, 863, 1777, 1818
NcoI	CCATGG	2 cut(s)	786, 1132
NdeII	GATC	2 cut(s)	148, 268
NlaIV	GGNNCC	5 cut(s)	498, 1483, 1866, 1888, 2062
NmuCI	GTSAC	1 cut(s)	781
NspI	RCATGY	2 cut(s)	1198, 1501
NspV	TTCGAA	1 cut(s)	445
PaeR7I	CTCGAG	1 cut(s)	1201
PagI	TCATGA	2 cut(s)	724, 1746
PciI	ACATGT	1 cut(s)	1497
PciSI	GCTCTTC	1 cut(s)	300
PctI	GAATGC	4 cut(s)	388, 756, 1028, 1903
PdmI	GAANNNNTTC	1 cut(s)	765
PfeI	GAWTC	5 cut(s)	238, 578, 917, 1166, 2155
PfoI	TCCNGGA	1 cut(s)	1827
PkrI	GCNGC	4 cut(s)	637, 1041, 1225, 1696
Ple19I	CGATCG	1 cut(s)	271
PleI	GAGTC	1 cut(s)	829
PpsI	GAGTC	1 cut(s)	829
PscI	ACATGT	1 cut(s)	1497
Psp124BI	GAGCTC	3 cut(s)	218, 645, 1624
Psp6I	CCWGG	3 cut(s)	567, 1320, 1827
PspEI	GGTNACC	1 cut(s)	781
PspGI	CCWGG	3 cut(s)	567, 1320, 1827
PspN4I	GGNNCC	5 cut(s)	498, 1483, 1866, 1888, 2062
PspPI	GGNCC	2 cut(s)	565, 2061
PspXI	VCTCGAGB	1 cut(s)	1201
PstI	CTGCAG	1 cut(s)	625
PstNI	CAGNNNCTG	3 cut(s)	103, 1018, 2158
PvuI	CGATCG	1 cut(s)	271
RsaI	GTAC	9 cut(s)	131, 611, 1017, 1199, 1296, 1412, 1502, 1702, 1937
RsaNI	GTAC	9 cut(s)	130, 610, 1016, 1198, 1295, 1411, 1501, 1701, 1936
RseI	CAYNNNNRTG	4 cut(s)	834, 858, 1137, 1440
SacI	GAGCTC	3 cut(s)	218, 645, 1624
SapI	GCTCTTC	1 cut(s)	300
SaqAI	TTAA	7 cut(s)	171, 204, 558, 744, 843, 1542, 2013
SatI	GCNGC	4 cut(s)	636, 1040, 1224, 1695
Sau3AI	GATC	2 cut(s)	148, 268
Sau96I	GGNCC	2 cut(s)	565, 2061
SchI	GAGTC	1 cut(s)	830
ScrFI	CCNGG	3 cut(s)	569, 1322, 1829
SduI	GDGCHC	4 cut(s)	218, 290, 645, 1624
SfcI	CTRYAG	1 cut(s)	621
Sfr274I	CTCGAG	1 cut(s)	1201
SfuI	TTCGAA	1 cut(s)	445
SinI	GGWCC	1 cut(s)	2061
SlaI	CTCGAG	1 cut(s)	1201
SmiMI	CAYNNNNRTG	4 cut(s)	834, 858, 1137, 1440
SmlI	CTYRAG	2 cut(s)	1201, 1241
SmoI	CTYRAG	2 cut(s)	1201, 1241
SsiI	CCGC	7 cut(s)	563, 635, 638, 1042, 1224, 1485, 1977
SspMI	CTAG	5 cut(s)	224, 1058, 1380, 2037, 2130
SstI	GAGCTC	3 cut(s)	218, 645, 1624
StyD4I	CCNGG	3 cut(s)	567, 1320, 1827
StyI	CCWWGG	3 cut(s)	786, 1117, 1132
TaaI	ACNGT	2 cut(s)	1358, 1967
TaiI	ACGT	1 cut(s)	588
TaqI	TCGA	5 cut(s)	267, 271, 445, 605, 1202
TatI	WGTACW	6 cut(s)	129, 1197, 1294, 1500, 1700, 1935
TauI	GCSGC	2 cut(s)	638, 1226
TfiI	GAWTC	5 cut(s)	238, 578, 917, 1166, 2155
Tru1I	TTAA	7 cut(s)	171, 204, 558, 744, 843, 1542, 2013
Tru9I	TTAA	7 cut(s)	171, 204, 558, 744, 843, 1542, 2013
TscAI	CASTG	4 cut(s)	108, 295, 1790, 1884
TseFI	GTSAC	1 cut(s)	781
TseI	GCWGC	2 cut(s)	1039, 1694
Tsp45I	GTSAC	1 cut(s)	781
TspGWI	ACGGA	2 cut(s)	489, 859
TspRI	CASTG	4 cut(s)	108, 295, 1790, 1884
VpaK11BI	GGWCC	1 cut(s)	2061
XapI	RAATTY	3 cut(s)	1273, 2110, 2143
XceI	RCATGY	2 cut(s)	1198, 1501
XhoI	CTCGAG	1 cut(s)	1201
XmiI	GTMKAC	1 cut(s)	824
XmnI	GAANNNNTTC	1 cut(s)	765
XspI	CTAG	5 cut(s)	224, 1058, 1380, 2037, 2130

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.