MD15G1118800.v1.1

Carbon catabolite repressor protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005330
Physical Location & Seq
Forward (+)
8531611 .. 8537436
5826 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1118800.v1.1.491

Sequence Viewer

Length: 2457 bp
ATGTTTGTGACTAGGGACCCGGTGACTGCCATCCGGGAAACCGTTACCTTCCTTCCCGACAAAATGAGACACGCTCCTCCTCCTTCCCTCCAATTCCTCGCCGCTGCCGCCACCAACTCCGCCGTCACCGCCATGTCTTCGCGCCCTCCTCGAGGTGGTCGAAACCCGTGGCAGAGAGGCTTCTCCAACCGCCCCTACTCAGGCGGCGGTAGAAGCAACTACGTTACCGGAGACTCCCACTTCCGATCAGTCCGCGACGCCAATCTAGGGTCCCGGCAAGGCGATTCCGGACCCTTCCCCAACCAAACCTCCTATCGTCGGACTCCGCCGCCGTCTTTCGATCTCAACTACTTCCGCGGTCCTCCGCAGCTCGATCACAATCAACCGTACCGGCCGAATCAGCAATTTCGGCCTCCCCATCAGTTTCGGCCGCCTCAGCAATTCCGGCCTCGGCCGAAACCCTTGGACTATCGGAACTGGGAATTTGCCAAAGCGACGCCGTCCCCTACTTGTGAGAGGTTTACGGTGTTGTCATATAATATACTGGCTGATTACTTGGCAAACGATCACCGGAGTAAGCTATACTATCACATACCTCATCATATGATGGACTGGCAGTGGAGAAAGCGAAATTTGATTTTCGAGCTCGGGTTGTGGTCACCCGACATAATGTGCCTTCAGGAAGTTGATAAATTTGAGGACCTTGAGGAGGAGTTTAAGCTTAAAGGGTACATCGGCATTTGGAAGATGAGGACTGGTAATCCAGTTGATGGATGTGCAATTTTTTGGCGATCATCAAGATTCAAGTTGGTCCATGAGGAATCCATCGAGTTCAATAAGCTTGGACTTCGGGATAATGTTGCTCAAATATGCGTGCTGGAGTCAATGCGTCAGAATAGCACCAAAAATGTTGCAGCTCTACCAACAAGCTCAACAGGTACTAATAAAGTTGTCATTTGTAACATTCATGTGCTTTACAATCCTAGACGGGGAGAAATTAAGCTTGGCCAGGTCAGGGTTCTTTTGGATAGGGCTCATTCTGTTTCCAAAATCTGGAATGATGCTCCAATTGTTCTTTGTGGGGATTTCAATTGTACACCTAAGAGTCCGTTATACAACTTTATTTCAGAACAGAAGTTAGATTTGTCTGGAGTTGACAGAGATAAGGTATCTGGACAAGCTTCAGCTCAAATTTATGCACCAAGGTTATATGATCATAATTATGGGGCTGGGCCTGCTAGTCATATAGCTCAAGGTCCACCAATTGTAGATGGGAGGGAAGCTGGACTGAACTCGGGCAATCAGTCATTGGATATCCAGAACCCAAATAATCCAGAAAGTAGTGCAGAGAGTGTTCCATTAGTGAACAACTTGTCTCAGCCTCAAAGTACTGACATCTCAGACGTTTTTGATAAGTCTTGCCCCAATGTGCATTTTGGAAAAGATGACGGTTCCAAAGATGTTGATGTGAACCAAGAAACTAAGCAGAACTCTTTAGATGTCTCTGAGGTTGAAACTGGATCAGCATTCCATGTGACTGAGATGGGATACAAAGAAAAAGTGAATTTCACCTCTAATTCAAATCTTGGATTATCAAGCAGACATTTGAATTCAGATGTTTCCCTAGAGAAAGAAAGGATGAACTGTGATATCCATAAACATCTCTCTACGAGGGACAATGATTCTATTAGGGAAGGGATTGATCCAGATTCTGTGGTTCCTATCGACTCAGAAGTTCCTTTATCTAAGCTATCTAACCAGGCTTCCATTACCGATGCTACTAAAGGCTCTTCATTGGAAAACTTAGGGCATATGTCTTCCAATCCAGTTTCTGGTGTCGAGGAGGAAAGGCCTTCAGCCTCATATCAAGTGGACATATCATGTCCATCTGCTAGTGCTGACCTTGAATTGCAGAAAAATATGGAAAAGTTATCTCTTAGTGTGCTAGATGAAGCCATTCCGGCAGATGAAAATATAGCTGAAGATGATAAAACGTTTATATCTGCATTACACGATACGGAAGATGCCTTTCCATCTGATTTTACTTCTGTTACTCCTCCCAACTCAGCATTTTCAGCATCACCCAATGCGGAGAGAACCTATTATGATCCATCACTATGGACTCCAATGGAAATAGAAACTGCCACAGGCAATGCGGAACACACTCTTTTAGAACATCCTCTGAAGCTTAAGAGCACATATACTGAAGTTGAGGATTGTTCTGGCACCAGAGATTCAAGTGGGGAGCCCCTGGTGACCAGTTATAATAAATTATTCTTGGGCACGGTTGATTACATCTGGTGTTCCGAAGGTCTGCAGACTGTCAGGGTGCTTGCTCCTATTCCGAAGCAAGCTATGCAGTGGACTTCAGGGTTCCCAACCAAGAAATGGGGTAGTGATCATATTGCATTGGCTTCTGAGTTGGCATTCATCAACAATTCTGACCAATCATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

819

Amino Acids

91.24

Weight (kDa)

5.59

Isoelectric Point (pI)

44.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 219 - 405 4.7e-10 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2265
AbsI CCTCGAGG 1 cut(s) 150
AccB1I GGYRCC 1 cut(s) 2225
AccB7I CCANNNNNTGG 4 cut(s) 770, 1053, 1832, 2388
AccII CGCG 3 cut(s) 142, 255, 357
AccIII TCCGGA 1 cut(s) 287
AclI AACGTT 1 cut(s) 1994
AclWI GGATC 3 cut(s) 1528, 1697, 2102
AcoI YGGCCR 4 cut(s) 392, 428, 452, 1006
AcsI RAATTY 6 cut(s) 482, 631, 692, 1191, 1564, 1609
AcuI CTGAAG 7 cut(s) 662, 1167, 1839, 2001, 2204, 2226, 2352
AcyI GRCGYC 2 cut(s) 258, 497
AfaI GTAC 5 cut(s) 389, 731, 940, 1096, 1390
AflII CTTAAG 1 cut(s) 2189
AgsI TTSAA 8 cut(s) 805, 835, 1090, 1514, 1581, 1609, 1907, 2238
AjnI CCWGG 3 cut(s) 1008, 1758, 2250
AjuI GAANNNNNNNTTGG 2 cut(s) 987, 1019
Alw21I GWGCWC 2 cut(s) 648, 2198
Alw26I GTCTC 4 cut(s) 61, 225, 1380, 1507
AlwI GGATC 3 cut(s) 1528, 1697, 2102
AlwNI CAGNNNCTG 2 cut(s) 1712, 1832
Ama87I CYCGRG 3 cut(s) 150, 647, 1294
Aor13HI TCCGGA 1 cut(s) 287
AoxI GGCC 8 cut(s) 392, 410, 428, 446, 452, 1006, 1232, 1850
ApeKI GCWGC 3 cut(s) 104, 367, 914
ApoI RAATTY 6 cut(s) 482, 631, 692, 1191, 1564, 1609
Asp700I GAANNNNTTC 1 cut(s) 1956
AspLEI GCGC 1 cut(s) 144
AspS9I GGNCC 8 cut(s) 16, 270, 290, 359, 700, 811, 1232, 1256
AsuC2I CCSGG 3 cut(s) 20, 35, 274
AsuHPI GGTGA 7 cut(s) 34, 118, 560, 651, 1561, 2073, 2266
AvaI CYCGRG 3 cut(s) 150, 647, 1294
AvaII GGWCC 7 cut(s) 16, 270, 290, 359, 700, 811, 1256
BaeGI GKGCMC 1 cut(s) 2285
BalI TGGCCA 1 cut(s) 1008
BanI GGYRCC 1 cut(s) 2225
BanII GRGCYC 3 cut(s) 648, 1036, 2250
BbsI GAAGAC 2 cut(s) 129, 1809
Bbv12I GWGCWC 2 cut(s) 648, 2198
BbvCI CCTCAGC 1 cut(s) 435
BbvI GCAGC 3 cut(s) 91, 379, 926
BceAI ACGGC 3 cut(s) 107, 316, 484
BciT130I CCWGG 3 cut(s) 1010, 1760, 2252
BciVI GTATCC 1 cut(s) 1541
BclI TGATCA 2 cut(s) 1213, 2398
BcnI CCSGG 3 cut(s) 20, 35, 274
BcoDI GTCTC 4 cut(s) 61, 225, 1380, 1507
BfaI CTAG 7 cut(s) 12, 266, 984, 1239, 1625, 1893, 1946
BfmI CTRYAG 1 cut(s) 2315
BfrI CTTAAG 1 cut(s) 2189
BfuI GTATCC 1 cut(s) 1541
BglI GCCNNNNNGGC 1 cut(s) 1961
BisI GCNGC 8 cut(s) 102, 105, 108, 205, 329, 368, 431, 915
BlsI GCNGC 8 cut(s) 103, 106, 109, 206, 330, 369, 432, 916
BmcAI AGTACT 1 cut(s) 1390
Bme1390I CCNGG 6 cut(s) 20, 35, 274, 1010, 1760, 2252
Bme18I GGWCC 7 cut(s) 16, 270, 290, 359, 700, 811, 1256
BmeT110I CYCGRG 3 cut(s) 150, 647, 1294
BmgT120I GGNCC 8 cut(s) 16, 270, 290, 359, 700, 811, 1232, 1256
BmrFI CCNGG 6 cut(s) 20, 35, 274, 1010, 1760, 2252
BmrI ACTGGG 1 cut(s) 487
BmsI GCATC 4 cut(s) 1051, 1765, 2014, 2087
BmuI ACTGGG 1 cut(s) 487
BpiI GAAGAC 2 cut(s) 129, 1809
BplI GAGNNNNNCTC 2 cut(s) 58, 90
BpmI CTGGAG 2 cut(s) 899, 1170
Bpu10I CCTNAGC 1 cut(s) 435
BpuEI CTTGAG 2 cut(s) 725, 1236
BpuMI CCSGG 3 cut(s) 20, 35, 274
BsaBI GATNNNNATC 1 cut(s) 378
BsaHI GRCGYC 2 cut(s) 258, 497
BsaJI CCNNGG 6 cut(s) 167, 355, 449, 462, 1202, 2250
BsaWI WCCGGW 3 cut(s) 227, 287, 570
BsaXI ACNNNNNCTCC 2 cut(s) 293, 323
Bse118I RCCGGY 1 cut(s) 390
Bse1I ACTGG 8 cut(s) 482, 549, 617, 760, 764, 1522, 1826, 2259
Bse3DI GCAATG 1 cut(s) 2158
Bse8I GATNNNNATC 1 cut(s) 378
BseAI TCCGGA 1 cut(s) 287
BseBI CCWGG 3 cut(s) 1010, 1760, 2252
BseDI CCNNGG 6 cut(s) 167, 355, 449, 462, 1202, 2250
BseGI GGATG 4 cut(s) 30, 779, 1644, 2176
BseJI GATNNNNATC 1 cut(s) 378
BseMI GCAATG 1 cut(s) 2158
BseMII CTCAG 9 cut(s) 213, 449, 1391, 1413, 1497, 1530, 1743, 2079, 2409
BseNI ACTGG 8 cut(s) 482, 549, 617, 760, 764, 1522, 1826, 2259
BseRI GAGGAG 7 cut(s) 66, 69, 138, 722, 725, 1856, 2046
BseSI GKGCMC 1 cut(s) 2285
BseX3I CGGCCG 3 cut(s) 392, 428, 452
BseXI GCAGC 3 cut(s) 91, 379, 926
BseYI CCCAGC 1 cut(s) 1229
BsgI GTGCAG 1 cut(s) 1365
Bsh1236I CGCG 3 cut(s) 142, 255, 357
Bsh1285I CGRYCG 3 cut(s) 395, 431, 455
BshFI GGCC 8 cut(s) 394, 412, 430, 448, 454, 1008, 1234, 1852
BshNI GGYRCC 1 cut(s) 2225
BsiEI CGRYCG 3 cut(s) 395, 431, 455
BsiHKAI GWGCWC 2 cut(s) 648, 2198
BsiHKCI CYCGRG 3 cut(s) 150, 647, 1294
BsiSI CCGG 9 cut(s) 20, 34, 228, 274, 288, 391, 445, 571, 1961
BslFI GGGAC 4 cut(s) 29, 256, 487, 1688
BsmAI GTCTC 4 cut(s) 61, 225, 1380, 1507
BsmFI GGGAC 4 cut(s) 29, 256, 487, 1688
BsmI GAATGC 2 cut(s) 1526, 2426
BsnI GGCC 8 cut(s) 394, 412, 430, 448, 454, 1008, 1234, 1852
BsoBI CYCGRG 3 cut(s) 150, 647, 1294
Bsp1286I GDGCHC 5 cut(s) 648, 1036, 2198, 2250, 2285
Bsp13I TCCGGA 1 cut(s) 287
Bsp1407I TGTACA 1 cut(s) 1094
BspANI GGCC 8 cut(s) 394, 412, 430, 448, 454, 1008, 1234, 1852
BspCNI CTCAG 9 cut(s) 212, 448, 1390, 1412, 1498, 1531, 1742, 2078, 2410
BspEI TCCGGA 1 cut(s) 287
BspFNI CGCG 3 cut(s) 142, 255, 357
BspMAI CTGCAG 1 cut(s) 2319
BspPI GGATC 3 cut(s) 1528, 1697, 2102
BspQI GCTCTTC 1 cut(s) 1795
BspT107I GGYRCC 1 cut(s) 2225
BspTI CTTAAG 1 cut(s) 2189
BsrDI GCAATG 1 cut(s) 2158
BsrFI RCCGGY 1 cut(s) 390
BsrGI TGTACA 1 cut(s) 1094
BsrI ACTGG 8 cut(s) 482, 549, 617, 760, 764, 1522, 1826, 2259
BssAI RCCGGY 1 cut(s) 390
BssECI CCNNGG 6 cut(s) 167, 355, 449, 462, 1202, 2250
BssNI GRCGYC 2 cut(s) 258, 497
BssT1I CCWWGG 2 cut(s) 462, 1202
Bst2UI CCWGG 3 cut(s) 1010, 1760, 2252
Bst4CI ACNGT 7 cut(s) 43, 387, 526, 1451, 1646, 2287, 2323
Bst6I CTCTTC 1 cut(s) 1795
BstACI GRCGYC 2 cut(s) 258, 497
BstAFI CTTAAG 1 cut(s) 2189
BstAPI GCANNNNNTGC 1 cut(s) 2356
BstAUI TGTACA 1 cut(s) 1094
BstC8I GCNNGC 4 cut(s) 875, 1236, 2334, 2352
BstDSI CCRYGG 2 cut(s) 167, 355
BstEII GGTNACC 2 cut(s) 657, 2254
BstENI CCTNNNNNAGG 2 cut(s) 150, 707
BstF5I GGATG 4 cut(s) 30, 779, 1644, 2176
BstFNI CGCG 3 cut(s) 142, 255, 357
BstHHI GCGC 1 cut(s) 144
BstMAI GTCTC 4 cut(s) 61, 225, 1380, 1507
BstMCI CGRYCG 3 cut(s) 395, 431, 455
BstNI CCWGG 3 cut(s) 1010, 1760, 2252
BstPI GGTNACC 2 cut(s) 657, 2254
BstSCI CCNGG 6 cut(s) 18, 33, 272, 1008, 1758, 2250
BstSFI CTRYAG 1 cut(s) 2315
BstSLI GKGCMC 1 cut(s) 2285
BstUI CGCG 3 cut(s) 142, 255, 357
BstV1I GCAGC 3 cut(s) 91, 379, 926
BstV2I GAAGAC 2 cut(s) 129, 1809
BstXI CCANNNNNNTGG 1 cut(s) 2118
BstZI CGGCCG 3 cut(s) 392, 428, 452
BsuI GTATCC 1 cut(s) 1541
BsuRI GGCC 8 cut(s) 394, 412, 430, 448, 454, 1008, 1234, 1852
BtgI CCRYGG 2 cut(s) 167, 355
BtsCI GGATG 4 cut(s) 30, 779, 1644, 2176
BtsI GCAGTG 2 cut(s) 623, 2366
BtsIMutI CAGTG 2 cut(s) 623, 2366
Cac8I GCNNGC 4 cut(s) 875, 1236, 2334, 2352
CaiI CAGNNNCTG 2 cut(s) 1712, 1832
CfoI GCGC 1 cut(s) 144
Cfr10I RCCGGY 1 cut(s) 390
Cfr13I GGNCC 8 cut(s) 16, 270, 290, 359, 700, 811, 1232, 1256
Cfr42I CCGCGG 1 cut(s) 358
CseI GACGC 3 cut(s) 266, 505, 878
Csp6I GTAC 5 cut(s) 388, 730, 939, 1095, 1389
CviAII CATG 5 cut(s) 133, 815, 968, 1532, 1881
CviQI GTAC 5 cut(s) 388, 730, 939, 1095, 1389
EaeI YGGCCR 4 cut(s) 392, 428, 452, 1006
EagI CGGCCG 3 cut(s) 392, 428, 452
Eam1104I CTCTTC 1 cut(s) 1795
EarI CTCTTC 1 cut(s) 1795
EciI GGCGGA 2 cut(s) 109, 315
Ecl136II GAGCTC 1 cut(s) 646
EclXI CGGCCG 3 cut(s) 392, 428, 452
Eco130I CCWWGG 2 cut(s) 462, 1202
Eco147I AGGCCT 1 cut(s) 1852
Eco24I GRGCYC 3 cut(s) 648, 1036, 2250
Eco32I GATATC 2 cut(s) 1315, 1651
Eco47I GGWCC 7 cut(s) 16, 270, 290, 359, 700, 811, 1256
Eco52I CGGCCG 3 cut(s) 392, 428, 452
Eco53kI GAGCTC 1 cut(s) 646
Eco57I CTGAAG 7 cut(s) 662, 1167, 1839, 2001, 2204, 2226, 2352
Eco88I CYCGRG 3 cut(s) 150, 647, 1294
Eco91I GGTNACC 2 cut(s) 657, 2254
EcoICRI GAGCTC 1 cut(s) 646
EcoNI CCTNNNNNAGG 2 cut(s) 150, 707
EcoO109I RGGNCCY 3 cut(s) 16, 270, 700
EcoO65I GGTNACC 2 cut(s) 657, 2254
EcoRI GAATTC 1 cut(s) 1609
EcoRII CCWGG 3 cut(s) 1008, 1758, 2250
EcoRV GATATC 2 cut(s) 1315, 1651
EcoT14I CCWWGG 2 cut(s) 462, 1202
EcoT38I GRGCYC 3 cut(s) 648, 1036, 2250
ErhI CCWWGG 2 cut(s) 462, 1202
FaeI CATG 5 cut(s) 136, 818, 971, 1535, 1884
FaqI GGGAC 4 cut(s) 29, 256, 487, 1688
FatI CATG 5 cut(s) 132, 814, 967, 1531, 1880
FauNDI CATATG 2 cut(s) 603, 1812
FbaI TGATCA 2 cut(s) 1213, 2398
Fnu4HI GCNGC 8 cut(s) 102, 105, 108, 205, 329, 368, 431, 915
FokI GGATG 4 cut(s) 17, 786, 1651, 2163
FriOI GRGCYC 3 cut(s) 648, 1036, 2250
Fsp4HI GCNGC 8 cut(s) 102, 105, 108, 205, 329, 368, 431, 915
FspBI CTAG 7 cut(s) 12, 266, 984, 1239, 1625, 1893, 1946
GlaI GCGC 1 cut(s) 143
GluI GCNGC 8 cut(s) 102, 105, 108, 205, 329, 368, 431, 915
GsaI CCCAGC 1 cut(s) 1233
GsuI CTGGAG 2 cut(s) 899, 1170
HaeIII GGCC 8 cut(s) 394, 412, 430, 448, 454, 1008, 1234, 1852
HapII CCGG 9 cut(s) 20, 34, 228, 274, 288, 391, 445, 571, 1961
HgaI GACGC 3 cut(s) 266, 505, 878
HhaI GCGC 1 cut(s) 144
Hin1I GRCGYC 2 cut(s) 258, 497
Hin1II CATG 5 cut(s) 136, 818, 971, 1535, 1884
Hin6I GCGC 1 cut(s) 142
HinP1I GCGC 1 cut(s) 142
HincII GTYRAC 1 cut(s) 1156
HindII GTYRAC 1 cut(s) 1156
HindIII AAGCTT 5 cut(s) 719, 839, 1001, 1179, 2186
HpaII CCGG 9 cut(s) 20, 34, 228, 274, 288, 391, 445, 571, 1961
HphI GGTGA 7 cut(s) 34, 118, 560, 651, 1561, 2073, 2266
Hpy166II GTNNAC 8 cut(s) 522, 1097, 1156, 1259, 1366, 1471, 1873, 2364
Hpy8I GTNNAC 8 cut(s) 522, 1097, 1156, 1259, 1366, 1471, 1873, 2364
Hpy99I CGWCG 3 cut(s) 260, 321, 499
HpyAV CCTTC 8 cut(s) 58, 62, 93, 304, 686, 1688, 1863, 2303
HpyCH4III ACNGT 7 cut(s) 43, 387, 526, 1451, 1646, 2287, 2323
HpyCH4IV ACGT 3 cut(s) 222, 1404, 1994
HpySE526I ACGT 3 cut(s) 222, 1404, 1994
Hsp92I GRCGYC 2 cut(s) 258, 497
Hsp92II CATG 5 cut(s) 136, 818, 971, 1535, 1884
HspAI GCGC 1 cut(s) 142
KflI GGGWCCC 2 cut(s) 16, 270
Kpn2I TCCGGA 1 cut(s) 287
Ksp22I TGATCA 2 cut(s) 1213, 2398
KspI CCGCGG 1 cut(s) 358
LguI GCTCTTC 1 cut(s) 1795
LmnI GCTCC 4 cut(s) 79, 1069, 2245, 2341
Lsp1109I GCAGC 3 cut(s) 91, 379, 926
LweI GCATC 4 cut(s) 1051, 1765, 2014, 2087
MaeI CTAG 7 cut(s) 12, 266, 984, 1239, 1625, 1893, 1946
MaeII ACGT 3 cut(s) 222, 1404, 1994
MboII GAAGA 6 cut(s) 129, 757, 1782, 1809, 1994, 2033
MfeI CAATTG 3 cut(s) 1068, 1090, 1263
MhlI GDGCHC 5 cut(s) 648, 1036, 2198, 2250, 2285
MlsI TGGCCA 1 cut(s) 1008
MluNI TGGCCA 1 cut(s) 1008
MlyI GAGTC 6 cut(s) 227, 316, 890, 1114, 1721, 2116
MmeI TCCRAC 2 cut(s) 210, 299
Mox20I TGGCCA 1 cut(s) 1008
MroI TCCGGA 1 cut(s) 287
MroXI GAANNNNTTC 1 cut(s) 1956
MscI TGGCCA 1 cut(s) 1008
MseI TTAA 4 cut(s) 717, 723, 999, 2190
MslI CAYNNNNRTG 4 cut(s) 131, 968, 1221, 2116
Msp20I TGGCCA 1 cut(s) 1008
MspA1I CMGCKG 2 cut(s) 104, 357
MspCI CTTAAG 1 cut(s) 2189
MspI CCGG 9 cut(s) 20, 34, 228, 274, 288, 391, 445, 571, 1961
MspR9I CCNGG 6 cut(s) 20, 35, 274, 1010, 1760, 2252
MunI CAATTG 3 cut(s) 1068, 1090, 1263
Mva1269I GAATGC 2 cut(s) 1526, 2426
MvaI CCWGG 3 cut(s) 1010, 1760, 2252
MvnI CGCG 3 cut(s) 142, 255, 357
NciI CCSGG 3 cut(s) 20, 35, 274
NdeI CATATG 2 cut(s) 603, 1812
NlaIII CATG 5 cut(s) 136, 818, 971, 1535, 1884
NmeAIII GCCGAG 1 cut(s) 430
NmuCI GTSAC 6 cut(s) 7, 22, 124, 657, 1534, 2254
PaeR7I CTCGAG 1 cut(s) 150
PceI AGGCCT 1 cut(s) 1852
PciSI GCTCTTC 1 cut(s) 1795
PcsI WCGNNNNNNNCGW 1 cut(s) 157
PctI GAATGC 2 cut(s) 1526, 2426
PdmI GAANNNNTTC 1 cut(s) 1956
PfeI GAWTC 7 cut(s) 284, 397, 801, 821, 1682, 1709, 2234
PflFI GACNNNGTC 1 cut(s) 499
PflMI CCANNNNNTGG 4 cut(s) 770, 1053, 1832, 2388
PfoI TCCNGGA 1 cut(s) 33
PkrI GCNGC 8 cut(s) 103, 106, 109, 206, 330, 369, 432, 916
PleI GAGTC 6 cut(s) 227, 316, 889, 1113, 1721, 2116
PpsI GAGTC 6 cut(s) 227, 316, 889, 1113, 1721, 2116
PpuMI RGGWCCY 3 cut(s) 16, 270, 700
PsiI TTATAA 1 cut(s) 2265
Psp124BI GAGCTC 1 cut(s) 648
Psp1406I AACGTT 1 cut(s) 1994
Psp5II RGGWCCY 3 cut(s) 16, 270, 700
Psp6I CCWGG 3 cut(s) 1008, 1758, 2250
PspEI GGTNACC 2 cut(s) 657, 2254
PspFI CCCAGC 1 cut(s) 1229
PspGI CCWGG 3 cut(s) 1008, 1758, 2250
PspPI GGNCC 8 cut(s) 16, 270, 290, 359, 700, 811, 1232, 1256
PspPPI RGGWCCY 3 cut(s) 16, 270, 700
PspXI VCTCGAGB 1 cut(s) 150
PstI CTGCAG 1 cut(s) 2319
PstNI CAGNNNCTG 2 cut(s) 1712, 1832
PsyI GACNNNGTC 1 cut(s) 499
RsaI GTAC 5 cut(s) 389, 731, 940, 1096, 1390
RsaNI GTAC 5 cut(s) 388, 730, 939, 1095, 1389
RseI CAYNNNNRTG 4 cut(s) 131, 968, 1221, 2116
SacI GAGCTC 1 cut(s) 648
SacII CCGCGG 1 cut(s) 358
SapI GCTCTTC 1 cut(s) 1795
SaqAI TTAA 4 cut(s) 717, 723, 999, 2190
SatI GCNGC 8 cut(s) 102, 105, 108, 205, 329, 368, 431, 915
Sau96I GGNCC 8 cut(s) 16, 270, 290, 359, 700, 811, 1232, 1256
ScaI AGTACT 1 cut(s) 1390
SchI GAGTC 6 cut(s) 227, 316, 890, 1114, 1721, 2116
ScrFI CCNGG 6 cut(s) 20, 35, 274, 1010, 1760, 2252
SduI GDGCHC 5 cut(s) 648, 1036, 2198, 2250, 2285
SfaNI GCATC 4 cut(s) 1051, 1765, 2014, 2087
SfcI CTRYAG 1 cut(s) 2315
Sfr274I CTCGAG 1 cut(s) 150
Sfr303I CCGCGG 1 cut(s) 358
SgrBI CCGCGG 1 cut(s) 358
SinI GGWCC 7 cut(s) 16, 270, 290, 359, 700, 811, 1256
SlaI CTCGAG 1 cut(s) 150
SmiMI CAYNNNNRTG 4 cut(s) 131, 968, 1221, 2116
SmlI CTYRAG 4 cut(s) 150, 704, 1251, 2189
SmoI CTYRAG 4 cut(s) 150, 704, 1251, 2189
SseBI AGGCCT 1 cut(s) 1852
SspMI CTAG 7 cut(s) 12, 266, 984, 1239, 1625, 1893, 1946
SstI GAGCTC 1 cut(s) 648
StuI AGGCCT 1 cut(s) 1852
StyD4I CCNGG 6 cut(s) 18, 33, 272, 1008, 1758, 2250
StyI CCWWGG 2 cut(s) 462, 1202
TaaI ACNGT 7 cut(s) 43, 387, 526, 1451, 1646, 2287, 2323
TaiI ACGT 3 cut(s) 225, 1407, 1997
TaqI TCGA 8 cut(s) 151, 160, 339, 372, 642, 828, 1725, 1839
TatI WGTACW 2 cut(s) 1094, 1388
TauI GCSGC 5 cut(s) 104, 110, 207, 331, 433
TfiI GAWTC 7 cut(s) 284, 397, 801, 821, 1682, 1709, 2234
Tru1I TTAA 4 cut(s) 717, 723, 999, 2190
Tru9I TTAA 4 cut(s) 717, 723, 999, 2190
TscAI CASTG 2 cut(s) 623, 2366
TseFI GTSAC 6 cut(s) 7, 22, 124, 657, 1534, 2254
TseI GCWGC 3 cut(s) 104, 367, 914
Tsp45I GTSAC 6 cut(s) 7, 22, 124, 657, 1534, 2254
TspDTI ATGAA 6 cut(s) 956, 1655, 1782, 1965, 1983, 2419
TspGWI ACGGA 2 cut(s) 1098, 2033
TspRI CASTG 2 cut(s) 623, 2366
Tth111I GACNNNGTC 1 cut(s) 499
Van91I CCANNNNNTGG 4 cut(s) 770, 1053, 1832, 2388
Vha464I CTTAAG 1 cut(s) 2189
VpaK11BI GGWCC 7 cut(s) 16, 270, 290, 359, 700, 811, 1256
XagI CCTNNNNNAGG 2 cut(s) 150, 707
XapI RAATTY 6 cut(s) 482, 631, 692, 1191, 1564, 1609
XcmI CCANNNNNNNNNTGG 1 cut(s) 2385
XhoI CTCGAG 1 cut(s) 150
XmnI GAANNNNTTC 1 cut(s) 1956
XspI CTAG 7 cut(s) 12, 266, 984, 1239, 1625, 1893, 1946
ZrmI AGTACT 1 cut(s) 1390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.