Rw6G034500

Carbon catabolite repressor protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0005330
Physical Location & Seq
Reverse (-)
59076150 .. 59081961
5812 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G034500.1

Sequence Viewer

Length: 2430 bp
ATGAGAACGAGTGTACCAACGGCCTTGATCCCAAGAACAGTGTACTACTCTAACATGAAGCGCCCTCCTCCTCCTCTTCAATACGTCGTCGTTTTAGCCACACGCGCCACCGCAATGTCTTCTCGCCCTCCCTTTCGAGGCGGACGGAACCAATGGAAGAGAGGCTACTCGAATCGTCCGTTTTCCGGCGGCGACAGAGCTCAGTACGTCACCGGAGACTCTCACATCCGGTCGGTCCAGGACGCCAATCTCGGATTCCGGCAAGGCGACGCCGGAAGCTTCACCAACCAACCCGGTCATCACCGTCCGTTTGGTCAGAACAATCAGTTCCGCCGACCTCCGCCGGCGGATCACAGCCAACACTACCGGCCTCACCAGCAATTCCGGCCACCTCAGCAGTCGCGGCAGCGGCATCAGAAGCCGTTGGATTATCGGAATTGGGAGTCTGCCAAAACCACACTGCCCCTTAATTCTGAGAAGTTTATAGTGCTTTCTTATAATATACTGGCTGATTACTTGGCCAATGGCCACCGGAGTAAGCTTTACTATCACATTCCCCGGCATATGATGGACTGGCAATGGAGGAAGAAGAATTTGATTTTCGAGCTTGGCTTGTGGTCAGCTGACATAATGTGCCTTCAGGAGGTTGATAGATTTCAGGAACTTCAGGACGAGTTGAGGCCTCGGGGGTACAGTGGCATATGGAAGATGCGAACTGGCAATCCAGTAGATGGCTGTGCGGTATTTTGGCGGTCATCAAGGTTTAAGCTGCTTCATGAGGAATGCATTGAGTTCAATAAGCTTGGTCTTCGGGATAATGTTGCTCAGATATGTGTGCTGGAGTCGTTGCGTCAAACTTCCAAGGAAAATAATGCAGCTCTACCAACAAGTTCACCAGATTCTAATAAAGTTGTTATTTGTAACATTCATGTTCTTTATAATCCTGGACGAGGAGAAATTAAGCTTGGGCAGGTCAGGGTGCTCCTGGATAAGGCTTGTGCTGTTTCCAAATTCTGGAATGATGCTCCCATTGTTCTTTGTGGGGATTTCAATTGTACTCCAAAGAGTCCATTATATAACTTTATTTCAGAGCAGAAGTTAGATTTGTCTGAAGTGGACAGAGATAAGGTATCAGGGCAAGCCTCAGCTGAAATTGGTCCACCTAGGTCATTTAATCCTAATTATAGGGGTCAGCATGCTAGTAATTTCGTTCGAGGTTCACCAATGGTAGAATGGAAGGAAGTTAACATAAACATGAATGATTTAACCTCAGATCCACAGAACCTGAATAATCCAGAGAGCAGTGTGGAGGATGCCGGTGGGCATGATGCTGAAGCAAACCTGGAATCTAAGCAGAATGCTGTGGATGTCTGTAAGGTTGAAATTGGATTTGAGGAACATCCAAGTGATGGTGACTTGTCTGTAAATTTGAGTGATGAGTTTCATAATTGTTCTCCTGCAGTATCTTCCAATTCTGAAAAGGTTAACTCTGATGTGACTGACATTAGATACAAAGACAGTGTGGAGTACACCTCTAATTCAAATGATGACTCAAATGTTTGTGTAGAAAGAGAAAGCATGAACTGTAGTATCCTTAAACTTTCCTCTACAAAGGACAATGTAACTACTGGCGAGGGTTGTGATACAAATTATGTGGATCCTTTCAGCTCTAAAACTCCTTCGTCTGAGGTCTCTGGTCAGACTTTGTTTGCTGATGCTACTGAAGGCCCCCGATTGGGGAACTTGAGACATTTGCCATCCAAAGAAATTTTTGTTGTTGAGAAGTCAGCCCAACATGAAGTCAATATCACAAGTGCATCTACTAGTGTTGACTTTGAAGTGCAGAAAAATTTGGAAAAAATGTCTCTTACTGAGCTTGATGAAGCCATACCAGAAGGTGGAAACACAGTTGAAGATGATAGTACATTTTTATCTGCATTACACAACACTGGAGATGGCTTCGCATCTGATTTTGCTTCTGATCCTCCTAAGTACATGTTGCCTGCATCAACCGATAGGATTGAGGATGAATTATCTCCAGGATTGGATTCAGAGACAGTTGGTGTGGAGAAAACTATCTATGATCCATCACTATGGACTCCAATGGAGATAGAAACTGCTACAGGCAATGGGGAGTGCACTGATTTAGAACATCCTTTGAAGCTTAAGAGCACATATACTGAAGTTGAGGATTGTACTGGGACTAGGGACTCAAATGGAGAGCCCCTGGTGACCAGTTATAATAGGTGTTTCTTCGGGACAGTTGATTACATCTGGCGATCTGAAGGTCTGCAAACTGTCAGGGTCCTTGCTCCAATTCCAAAACATGCTATGCAATGGACTTCAGGATTCCCAACCAGGAAATGGGGTAGCGATCACATTGCCTTGGCTACTGAGTTGGTGTTCATGAAGTGCTCTGACCAATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

809

Amino Acids

90.75

Weight (kDa)

5.49

Isoelectric Point (pI)

47.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 206 - 394 4e-09 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 498, 939, 2241
Acc36I ACCTGC 1 cut(s) 961
AccB7I CCANNNNNTGG 5 cut(s) 731, 1014, 1409, 1898, 2364
AccII CGCG 2 cut(s) 105, 403
AclWI GGATC 7 cut(s) 22, 357, 1268, 1652, 1665, 1976, 2078
AcoI YGGCCR 3 cut(s) 386, 519, 526
AcsI RAATTY 5 cut(s) 592, 1010, 1426, 1767, 1849
AcuI CTGAAG 8 cut(s) 623, 650, 1131, 1353, 1743, 2202, 2304, 2328
AcyI GRCGYC 2 cut(s) 243, 270
AfaI GTAC 9 cut(s) 15, 44, 206, 692, 1057, 1529, 1924, 1994, 2197
AflII CTTAAG 1 cut(s) 2165
AflIII ACRYGT 1 cut(s) 1995
AgsI TTSAA 8 cut(s) 80, 796, 1051, 1382, 1542, 1838, 1913, 2161
AhlI ACTAGT 1 cut(s) 1823
AjnI CCWGG 7 cut(s) 237, 943, 984, 1341, 2038, 2226, 2357
AjuI GAANNNNNNNTTGG 4 cut(s) 239, 271, 948, 980
AloI GAACNNNNNNTCC 2 cut(s) 706, 738
Alw21I GWGCWC 5 cut(s) 202, 984, 2141, 2174, 2417
Alw26I GTCTC 5 cut(s) 210, 1696, 1741, 1869, 2048
Alw44I GTGCAC 1 cut(s) 2137
AlwI GGATC 7 cut(s) 22, 357, 1268, 1652, 1665, 1976, 2078
AlwNI CAGNNNCTG 1 cut(s) 1285
Ama87I CYCGRG 1 cut(s) 684
AoxI GGCC 7 cut(s) 21, 368, 386, 519, 526, 680, 1726
ApaLI GTGCAC 1 cut(s) 2137
ApeKI GCWGC 3 cut(s) 406, 769, 875
ApoI RAATTY 5 cut(s) 592, 1010, 1426, 1767, 1849
AspA2I CCTAGG 1 cut(s) 1163
AspLEI GCGC 2 cut(s) 63, 107
AspS9I GGNCC 4 cut(s) 235, 1157, 1727, 2305
AsuC2I CCSGG 2 cut(s) 294, 559
AsuHPI GGTGA 8 cut(s) 202, 274, 293, 365, 885, 1212, 1424, 2242
AvaI CYCGRG 1 cut(s) 684
AvaII GGWCC 3 cut(s) 235, 1157, 2305
AvrII CCTAGG 1 cut(s) 1163
BaeGI GKGCMC 1 cut(s) 2141
BaeI ACNNNNGTAYC 3 cut(s) 30, 1573, 1606
BalI TGGCCA 2 cut(s) 521, 528
BamHI GGATCC 1 cut(s) 1657
BanII GRGCYC 2 cut(s) 202, 2226
BarI GAAGNNNNNNTAC 2 cut(s) 149, 181
BbsI GAAGAC 2 cut(s) 111, 800
Bbv12I GWGCWC 5 cut(s) 202, 984, 2141, 2174, 2417
BbvCI CCTCAGC 2 cut(s) 393, 1144
BbvI GCAGC 3 cut(s) 418, 756, 887
BccI CCATC 6 cut(s) 562, 725, 1403, 1765, 1949, 2095
BceAI ACGGC 2 cut(s) 36, 406
BcgI CGANNNNNNTGC 2 cut(s) 2363, 2397
BciT130I CCWGG 7 cut(s) 239, 945, 986, 1343, 2040, 2228, 2359
BciVI GTATCC 1 cut(s) 1601
BcnI CCSGG 2 cut(s) 294, 559
BcoDI GTCTC 5 cut(s) 210, 1696, 1741, 1869, 2048
BcuI ACTAGT 1 cut(s) 1823
BfaI CTAG 4 cut(s) 1164, 1200, 1824, 2205
BfmI CTRYAG 3 cut(s) 1458, 1585, 2121
BfoI RGCGCY 1 cut(s) 64
BfrI CTTAAG 1 cut(s) 2165
BfuAI ACCTGC 1 cut(s) 961
BfuI GTATCC 1 cut(s) 1601
BisI GCNGC 6 cut(s) 190, 404, 407, 410, 770, 876
BlnI CCTAGG 1 cut(s) 1163
BlsI GCNGC 6 cut(s) 191, 405, 408, 411, 771, 877
Bme1390I CCNGG 9 cut(s) 239, 294, 559, 945, 986, 1343, 2040, 2228, 2359
Bme18I GGWCC 3 cut(s) 235, 1157, 2305
BmeT110I CYCGRG 1 cut(s) 684
BmgT120I GGNCC 4 cut(s) 235, 1157, 1727, 2305
BmiI GGNNCC 4 cut(s) 149, 1659, 1729, 2306
BmrFI CCNGG 9 cut(s) 239, 294, 559, 945, 986, 1343, 2040, 2228, 2359
BmrI ACTGGG 1 cut(s) 2208
BmsI GCATC 9 cut(s) 421, 699, 1012, 1303, 1318, 1705, 1826, 1973, 2015
BmuI ACTGGG 1 cut(s) 2208
BpiI GAAGAC 2 cut(s) 111, 800
BplI GAGNNNNNCTC 2 cut(s) 1517, 1549
BpmI CTGGAG 3 cut(s) 860, 1971, 2022
Bpu10I CCTNAGC 2 cut(s) 393, 1144
BpuEI CTTGAG 1 cut(s) 1765
BpuMI CCSGG 2 cut(s) 294, 559
BsaHI GRCGYC 2 cut(s) 243, 270
BsaI GGTCTC 1 cut(s) 1696
BsaJI CCNNGG 6 cut(s) 557, 683, 861, 1163, 2226, 2385
BsaWI WCCGGW 3 cut(s) 212, 228, 531
Bse118I RCCGGY 3 cut(s) 343, 366, 1316
Bse1I ACTGG 8 cut(s) 510, 578, 721, 725, 1633, 1954, 2203, 2235
Bse3DI GCAATG 5 cut(s) 120, 584, 2134, 2342, 2379
BseBI CCWGG 7 cut(s) 239, 945, 986, 1343, 2040, 2228, 2359
BseDI CCNNGG 6 cut(s) 557, 683, 861, 1163, 2226, 2385
BseGI GGATG 7 cut(s) 225, 1318, 1372, 1399, 1757, 2032, 2152
BseMI GCAATG 5 cut(s) 120, 584, 2134, 2342, 2379
BseMII CTCAG 9 cut(s) 215, 407, 465, 839, 1158, 1284, 1677, 1863, 2385
BseNI ACTGG 8 cut(s) 510, 578, 721, 725, 1633, 1954, 2203, 2235
BseRI GAGGAG 4 cut(s) 57, 60, 63, 966
BseSI GKGCMC 1 cut(s) 2141
BseXI GCAGC 3 cut(s) 418, 756, 887
BsgI GTGCAG 1 cut(s) 1862
Bsh1236I CGCG 2 cut(s) 105, 403
Bsh1285I CGRYCG 1 cut(s) 233
BshFI GGCC 7 cut(s) 23, 370, 388, 521, 528, 682, 1728
BsiEI CGRYCG 1 cut(s) 233
BsiHKAI GWGCWC 5 cut(s) 202, 984, 2141, 2174, 2417
BsiHKCI CYCGRG 1 cut(s) 684
BslFI GGGAC 3 cut(s) 2215, 2222, 2272
BsmAI GTCTC 5 cut(s) 210, 1696, 1741, 1869, 2048
BsmFI GGGAC 3 cut(s) 2215, 2222, 2272
BsmI GAATGC 2 cut(s) 788, 1363
BsnI GGCC 7 cut(s) 23, 370, 388, 521, 528, 682, 1728
Bso31I GGTCTC 1 cut(s) 1696
BsoBI CYCGRG 1 cut(s) 684
Bsp1286I GDGCHC 6 cut(s) 202, 984, 2141, 2174, 2226, 2417
Bsp143I GATC 8 cut(s) 27, 349, 1273, 1657, 1981, 2083, 2279, 2374
BspANI GGCC 7 cut(s) 23, 370, 388, 521, 528, 682, 1728
BspCNI CTCAG 9 cut(s) 214, 406, 466, 838, 1157, 1283, 1678, 1864, 2386
BspFNI CGCG 2 cut(s) 105, 403
BspHI TCATGA 2 cut(s) 775, 2406
BspLI GGNNCC 4 cut(s) 149, 1659, 1729, 2306
BspMAI CTGCAG 1 cut(s) 1462
BspMI ACCTGC 1 cut(s) 961
BspPI GGATC 7 cut(s) 22, 357, 1268, 1652, 1665, 1976, 2078
BspTI CTTAAG 1 cut(s) 2165
BspTNI GGTCTC 1 cut(s) 1696
BsrDI GCAATG 5 cut(s) 120, 584, 2134, 2342, 2379
BsrFI RCCGGY 3 cut(s) 343, 366, 1316
BsrI ACTGG 8 cut(s) 510, 578, 721, 725, 1633, 1954, 2203, 2235
BssAI RCCGGY 3 cut(s) 343, 366, 1316
BssECI CCNNGG 6 cut(s) 557, 683, 861, 1163, 2226, 2385
BssMI GATC 8 cut(s) 27, 349, 1273, 1657, 1981, 2083, 2279, 2374
BssNI GRCGYC 2 cut(s) 243, 270
BssT1I CCWWGG 3 cut(s) 861, 1163, 2385
Bst2UI CCWGG 7 cut(s) 239, 945, 986, 1343, 2040, 2228, 2359
Bst4CI ACNGT 9 cut(s) 40, 305, 695, 1520, 1586, 1909, 2059, 2263, 2299
Bst6I CTCTTC 2 cut(s) 81, 152
BstACI GRCGYC 2 cut(s) 243, 270
BstAFI CTTAAG 1 cut(s) 2165
BstC8I GCNNGC 4 cut(s) 345, 1140, 1197, 2004
BstEII GGTNACC 1 cut(s) 2230
BstENI CCTNNNNNAGG 3 cut(s) 641, 948, 989
BstF5I GGATG 7 cut(s) 225, 1318, 1372, 1399, 1757, 2032, 2152
BstFNI CGCG 2 cut(s) 105, 403
BstH2I RGCGCY 1 cut(s) 64
BstHHI GCGC 2 cut(s) 63, 107
BstKTI GATC 8 cut(s) 30, 352, 1276, 1660, 1984, 2086, 2282, 2377
BstMAI GTCTC 5 cut(s) 210, 1696, 1741, 1869, 2048
BstMBI GATC 8 cut(s) 27, 349, 1273, 1657, 1981, 2083, 2279, 2374
BstMCI CGRYCG 1 cut(s) 233
BstMWI GCNNNNNNNGC 7 cut(s) 104, 376, 385, 394, 403, 409, 418
BstNI CCWGG 7 cut(s) 239, 945, 986, 1343, 2040, 2228, 2359
BstNSI RCATGY 3 cut(s) 1199, 1999, 2330
BstPI GGTNACC 1 cut(s) 2230
BstSCI CCNGG 9 cut(s) 237, 292, 557, 943, 984, 1341, 2038, 2226, 2357
BstSFI CTRYAG 3 cut(s) 1458, 1585, 2121
BstSLI GKGCMC 1 cut(s) 2141
BstUI CGCG 2 cut(s) 105, 403
BstV1I GCAGC 3 cut(s) 418, 756, 887
BstV2I GAAGAC 2 cut(s) 111, 800
BstX2I RGATCY 2 cut(s) 1273, 1657
BstXI CCANNNNNNTGG 1 cut(s) 2094
BstYI RGATCY 2 cut(s) 1273, 1657
BsuI GTATCC 1 cut(s) 1601
BsuRI GGCC 7 cut(s) 23, 370, 388, 521, 528, 682, 1728
BtsCI GGATG 7 cut(s) 225, 1318, 1372, 1399, 1757, 2032, 2152
BtsI GCAGTG 2 cut(s) 458, 1309
BtsIMutI CAGTG 7 cut(s) 45, 458, 700, 1309, 1525, 1947, 2139
BveI ACCTGC 1 cut(s) 961
Cac8I GCNNGC 4 cut(s) 345, 1140, 1197, 2004
CaiI CAGNNNCTG 1 cut(s) 1285
CciI TCATGA 2 cut(s) 775, 2406
CfoI GCGC 2 cut(s) 63, 107
Cfr10I RCCGGY 3 cut(s) 343, 366, 1316
Cfr13I GGNCC 4 cut(s) 235, 1157, 1727, 2305
CseI GACGC 3 cut(s) 251, 278, 839
Csp6I GTAC 9 cut(s) 14, 43, 205, 691, 1056, 1528, 1923, 1993, 2196
CspCI CAANNNNNGTGG 2 cut(s) 1635, 1670
CviQI GTAC 9 cut(s) 14, 43, 205, 691, 1056, 1528, 1923, 1993, 2196
DpnI GATC 8 cut(s) 29, 351, 1275, 1659, 1983, 2085, 2281, 2376
DpnII GATC 8 cut(s) 27, 349, 1273, 1657, 1981, 2083, 2279, 2374
EaeI YGGCCR 3 cut(s) 386, 519, 526
Eam1104I CTCTTC 2 cut(s) 81, 152
EarI CTCTTC 2 cut(s) 81, 152
EciI GGCGGA 4 cut(s) 156, 320, 330, 362
Ecl136II GAGCTC 1 cut(s) 200
Eco130I CCWWGG 3 cut(s) 861, 1163, 2385
Eco147I AGGCCT 1 cut(s) 682
Eco24I GRGCYC 2 cut(s) 202, 2226
Eco31I GGTCTC 1 cut(s) 1696
Eco47I GGWCC 3 cut(s) 235, 1157, 2305
Eco53kI GAGCTC 1 cut(s) 200
Eco57I CTGAAG 8 cut(s) 623, 650, 1131, 1353, 1743, 2202, 2304, 2328
Eco88I CYCGRG 1 cut(s) 684
Eco91I GGTNACC 1 cut(s) 2230
EcoICRI GAGCTC 1 cut(s) 200
EcoNI CCTNNNNNAGG 3 cut(s) 641, 948, 989
EcoO109I RGGNCCY 2 cut(s) 1727, 2305
EcoO65I GGTNACC 1 cut(s) 2230
EcoRII CCWGG 7 cut(s) 237, 943, 984, 1341, 2038, 2226, 2357
EcoT14I CCWWGG 3 cut(s) 861, 1163, 2385
EcoT22I ATGCAT 1 cut(s) 788
EcoT38I GRGCYC 2 cut(s) 202, 2226
ErhI CCWWGG 3 cut(s) 861, 1163, 2385
FaqI GGGAC 3 cut(s) 2215, 2222, 2272
FauNDI CATATG 2 cut(s) 564, 701
Fnu4HI GCNGC 6 cut(s) 190, 404, 407, 410, 770, 876
FokI GGATG 7 cut(s) 212, 1325, 1379, 1386, 1744, 2039, 2139
FriOI GRGCYC 2 cut(s) 202, 2226
Fsp4HI GCNGC 6 cut(s) 190, 404, 407, 410, 770, 876
FspBI CTAG 4 cut(s) 1164, 1200, 1824, 2205
GlaI GCGC 2 cut(s) 62, 106
GluI GCNGC 6 cut(s) 190, 404, 407, 410, 770, 876
GsuI CTGGAG 3 cut(s) 860, 1971, 2022
HaeII RGCGCY 1 cut(s) 64
HaeIII GGCC 7 cut(s) 23, 370, 388, 521, 528, 682, 1728
HgaI GACGC 3 cut(s) 251, 278, 839
HhaI GCGC 2 cut(s) 63, 107
Hin1I GRCGYC 2 cut(s) 243, 270
Hin6I GCGC 2 cut(s) 61, 105
HinP1I GCGC 2 cut(s) 61, 105
HincII GTYRAC 3 cut(s) 1246, 1486, 1831
HindII GTYRAC 3 cut(s) 1246, 1486, 1831
HindIII AAGCTT 5 cut(s) 277, 539, 800, 962, 2162
HpaI GTTAAC 2 cut(s) 1246, 1486
HphI GGTGA 8 cut(s) 202, 274, 293, 365, 885, 1212, 1424, 2242
Hpy99I CGWCG 3 cut(s) 89, 92, 272
HpyAV CCTTC 6 cut(s) 647, 1231, 1689, 1718, 1889, 2279
HpyCH4III ACNGT 9 cut(s) 40, 305, 695, 1520, 1586, 1909, 2059, 2263, 2299
HpyCH4IV ACGT 2 cut(s) 84, 207
HpyF10VI GCNNNNNNNGC 7 cut(s) 104, 376, 385, 394, 403, 409, 418
HpySE526I ACGT 2 cut(s) 84, 207
Hsp92I GRCGYC 2 cut(s) 243, 270
HspAI GCGC 2 cut(s) 61, 105
KroI GCCGGC 1 cut(s) 343
KroNI GCCGGC 1 cut(s) 345
KspAI GTTAAC 2 cut(s) 1246, 1486
Kzo9I GATC 8 cut(s) 27, 349, 1273, 1657, 1981, 2083, 2279, 2374
LmnI GCTCC 3 cut(s) 987, 1030, 2317
Lsp1109I GCAGC 3 cut(s) 418, 756, 887
LweI GCATC 9 cut(s) 421, 699, 1012, 1303, 1318, 1705, 1826, 1973, 2015
MaeI CTAG 4 cut(s) 1164, 1200, 1824, 2205
MaeII ACGT 2 cut(s) 84, 207
MaeIII GTNAC 6 cut(s) 208, 920, 1412, 1495, 1621, 2230
MalI GATC 8 cut(s) 29, 351, 1275, 1659, 1983, 2085, 2281, 2376
MboI GATC 8 cut(s) 27, 349, 1273, 1657, 1981, 2083, 2279, 2374
MfeI CAATTG 1 cut(s) 1051
MflI RGATCY 2 cut(s) 1273, 1657
MhlI GDGCHC 6 cut(s) 202, 984, 2141, 2174, 2226, 2417
MlsI TGGCCA 2 cut(s) 521, 528
MluNI TGGCCA 2 cut(s) 521, 528
MlyI GAGTC 7 cut(s) 212, 452, 851, 1075, 1544, 2092, 2204
MmeI TCCRAC 1 cut(s) 405
Mox20I TGGCCA 2 cut(s) 521, 528
Mph1103I ATGCAT 1 cut(s) 788
MreI CGCCGGCG 1 cut(s) 343
MroNI GCCGGC 1 cut(s) 343
MscI TGGCCA 2 cut(s) 521, 528
MseI TTAA 9 cut(s) 468, 765, 960, 1173, 1245, 1265, 1485, 1596, 2166
MslI CAYNNNNRTG 4 cut(s) 113, 1253, 1404, 2092
Msp20I TGGCCA 2 cut(s) 521, 528
MspA1I CMGCKG 3 cut(s) 409, 623, 1148
MspCI CTTAAG 1 cut(s) 2165
MspR9I CCNGG 9 cut(s) 239, 294, 559, 945, 986, 1343, 2040, 2228, 2359
MunI CAATTG 1 cut(s) 1051
Mva1269I GAATGC 2 cut(s) 788, 1363
MvaI CCWGG 7 cut(s) 239, 945, 986, 1343, 2040, 2228, 2359
MvnI CGCG 2 cut(s) 105, 403
MwoI GCNNNNNNNGC 7 cut(s) 104, 376, 385, 394, 403, 409, 418
NaeI GCCGGC 1 cut(s) 345
NciI CCSGG 2 cut(s) 294, 559
NdeI CATATG 2 cut(s) 564, 701
NdeII GATC 8 cut(s) 27, 349, 1273, 1657, 1981, 2083, 2279, 2374
NgoMIV GCCGGC 1 cut(s) 343
NlaIV GGNNCC 4 cut(s) 149, 1659, 1729, 2306
NmuCI GTSAC 4 cut(s) 208, 1412, 1495, 2230
NsiI ATGCAT 1 cut(s) 788
NspI RCATGY 3 cut(s) 1199, 1999, 2330
PaeI GCATGC 1 cut(s) 1199
PagI TCATGA 2 cut(s) 775, 2406
PceI AGGCCT 1 cut(s) 682
PciI ACATGT 1 cut(s) 1995
PcsI WCGNNNNNNNCGW 1 cut(s) 176
PctI GAATGC 2 cut(s) 788, 1363
PdiI GCCGGC 1 cut(s) 345
PfeI GAWTC 6 cut(s) 172, 255, 899, 1346, 2048, 2349
PflMI CCANNNNNTGG 5 cut(s) 731, 1014, 1409, 1898, 2364
PfoI TCCNGGA 4 cut(s) 237, 943, 984, 2038
PkrI GCNGC 6 cut(s) 191, 405, 408, 411, 771, 877
PleI GAGTC 7 cut(s) 212, 451, 850, 1074, 1544, 2092, 2204
PpsI GAGTC 7 cut(s) 212, 451, 850, 1074, 1544, 2092, 2204
PpuMI RGGWCCY 1 cut(s) 2305
PscI ACATGT 1 cut(s) 1995
PsiI TTATAA 3 cut(s) 498, 939, 2241
Psp124BI GAGCTC 1 cut(s) 202
Psp5II RGGWCCY 1 cut(s) 2305
Psp6I CCWGG 7 cut(s) 237, 943, 984, 1341, 2038, 2226, 2357
PspEI GGTNACC 1 cut(s) 2230
PspGI CCWGG 7 cut(s) 237, 943, 984, 1341, 2038, 2226, 2357
PspN4I GGNNCC 4 cut(s) 149, 1659, 1729, 2306
PspPI GGNCC 4 cut(s) 235, 1157, 1727, 2305
PspPPI RGGWCCY 1 cut(s) 2305
PstI CTGCAG 1 cut(s) 1462
PstNI CAGNNNCTG 1 cut(s) 1285
PsuI RGATCY 2 cut(s) 1273, 1657
PvuII CAGCTG 2 cut(s) 623, 1148
RsaI GTAC 9 cut(s) 15, 44, 206, 692, 1057, 1529, 1924, 1994, 2197
RsaNI GTAC 9 cut(s) 14, 43, 205, 691, 1056, 1528, 1923, 1993, 2196
RseI CAYNNNNRTG 4 cut(s) 113, 1253, 1404, 2092
SacI GAGCTC 1 cut(s) 202
SaqAI TTAA 9 cut(s) 468, 765, 960, 1173, 1245, 1265, 1485, 1596, 2166
SatI GCNGC 6 cut(s) 190, 404, 407, 410, 770, 876
Sau3AI GATC 8 cut(s) 27, 349, 1273, 1657, 1981, 2083, 2279, 2374
Sau96I GGNCC 4 cut(s) 235, 1157, 1727, 2305
SchI GAGTC 7 cut(s) 212, 452, 851, 1075, 1544, 2092, 2204
ScrFI CCNGG 9 cut(s) 239, 294, 559, 945, 986, 1343, 2040, 2228, 2359
SduI GDGCHC 6 cut(s) 202, 984, 2141, 2174, 2226, 2417
SfaNI GCATC 9 cut(s) 421, 699, 1012, 1303, 1318, 1705, 1826, 1973, 2015
SfcI CTRYAG 3 cut(s) 1458, 1585, 2121
SgrAI CRCCGGYG 1 cut(s) 343
SinI GGWCC 3 cut(s) 235, 1157, 2305
SmiMI CAYNNNNRTG 4 cut(s) 113, 1253, 1404, 2092
SmlI CTYRAG 2 cut(s) 1744, 2165
SmoI CTYRAG 2 cut(s) 1744, 2165
SpeI ACTAGT 1 cut(s) 1823
SphI GCATGC 1 cut(s) 1199
SseBI AGGCCT 1 cut(s) 682
SspMI CTAG 4 cut(s) 1164, 1200, 1824, 2205
SstI GAGCTC 1 cut(s) 202
StuI AGGCCT 1 cut(s) 682
StyD4I CCNGG 9 cut(s) 237, 292, 557, 943, 984, 1341, 2038, 2226, 2357
StyI CCWWGG 3 cut(s) 861, 1163, 2385
TaaI ACNGT 9 cut(s) 40, 305, 695, 1520, 1586, 1909, 2059, 2263, 2299
TaiI ACGT 2 cut(s) 87, 210
TaqI TCGA 4 cut(s) 136, 170, 603, 1213
TaqII GACCGA 1 cut(s) 223
TatI WGTACW 6 cut(s) 42, 1055, 1527, 1922, 1992, 2195
TauI GCSGC 3 cut(s) 192, 406, 412
TfiI GAWTC 6 cut(s) 172, 255, 899, 1346, 2048, 2349
Tru1I TTAA 9 cut(s) 468, 765, 960, 1173, 1245, 1265, 1485, 1596, 2166
Tru9I TTAA 9 cut(s) 468, 765, 960, 1173, 1245, 1265, 1485, 1596, 2166
TscAI CASTG 7 cut(s) 45, 465, 700, 1309, 1525, 1954, 2146
TseFI GTSAC 4 cut(s) 208, 1412, 1495, 2230
TseI GCWGC 3 cut(s) 406, 769, 875
Tsp45I GTSAC 4 cut(s) 208, 1412, 1495, 2230
TspGWI ACGGA 3 cut(s) 160, 168, 297
TspRI CASTG 7 cut(s) 45, 465, 700, 1309, 1525, 1954, 2146
Van91I CCANNNNNTGG 5 cut(s) 731, 1014, 1409, 1898, 2364
Vha464I CTTAAG 1 cut(s) 2165
VneI GTGCAC 1 cut(s) 2137
VpaK11BI GGWCC 3 cut(s) 235, 1157, 2305
XagI CCTNNNNNAGG 3 cut(s) 641, 948, 989
XapI RAATTY 5 cut(s) 592, 1010, 1426, 1767, 1849
XceI RCATGY 3 cut(s) 1199, 1999, 2330
XcmI CCANNNNNNNNNTGG 2 cut(s) 1230, 2361
XmaJI CCTAGG 1 cut(s) 1163
XspI CTAG 4 cut(s) 1164, 1200, 1824, 2205
Zsp2I ATGCAT 1 cut(s) 788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.