RLG00000011486

Carbon catabolite repressor protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0005330
Physical Location & Seq
Forward (+)
9855996 .. 9861550
5555 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011486

Sequence Viewer

Length: 2403 bp
ATGAAGCGCCCTCCTCCTTCTCTTCAATACGTCGTCGTTTTAGCCACACACGCCACCGCAATGTCTTCTCGCCCTCCCTTTCGAGGCGGACGGAACCAATGGAAGAGAGGCTACTCGAATCGTCCGTTTTCCGGCGGCGACAGAGCTCAGTACGTCACCGGAGACTCGCACATCCGGTCGGTACAGGAAGCCAATCTCGGATTCCGGCAAGGCGACGCCGGAAGCTTCACCAACCAACCCGGTCATCACCGTCCGTTTAGTCAAAACAATCAGTTCCGTCGACCTCCGCCGTCGGATCACAGCCAACACTACCGGCCTCACAAGCAATTCCGGCCACCTCAGCAGTCGCGGCAGCGGCATCAGAAGCCGTTGGATTATCGAAATTGGGAGTATGCCAAAACCACACTGCCCCCTAATTCTGAGAAGTTTATAGTGCTTTCTTATAATATACTGGCTGATTACTTGGCCAATGGCCACCGGAGTAAGCTTTACTATCACATTCCCCGGCATATGATGGACTGGCAATGGAGGAAGAAGAATTTGATTTTCGAGCTTGGCTTATGGTCAGCTGACATAATGTGCTTTCAGGAGGTTGATAGATTTCAGGAACTTCAGGACGAGTTGAGGCCTCGGGGGTACAGTGGCATATGGAAGATGCGAACTGGCAATCCAGTAGATGGCTGTGCGGTTTTTTGGCGGTCATCAAGGCTTAAGCTGCTTCATGAGGAATGTATTGAGTTCAATAAGCTTGGTCTTCGGGATAATATTGCTCAGATATGTGTGCTGGAGTCGTTGCGTCAAACTTCCAAGGATAATAATGCAGCTTTATCAACAAGCTCACCAGATTCTAATAAAGTTGTTATTTGTAACATTCATGTTCTTTATAATCCTGGACGAGGAGAAATTAAGCTTGGCCAGGTCAGGGTGCTCCTGGATAAGGCTTGTGCTGTTTCCAAATTCTGGAATGATGCTCCCATTGTTCTTTGTGGGGATTTCAATTGTACTCCAAAGAGTCCATTATATAACTTTATTTCAGAGCAGAAGTTAGATTTGTCTGAAGTGGACAGAGATAAGGTATCAGGGCAAGCTTCAGCTGAAATTGGCCCACCTAGGTCATTTAATCCTAATTATAGGGGTCAGCATGCTAGTAATTTCGTTCAAGGTTCACCAATGGTAGAATGGAAGGAAGCTAACATAAACATGAATGATTTAACCTCGGATCCACAGAACCTGAATAATCCAGAAAGCAGTGTGGAGGGTGCCGGTGGGCATGATGCTGAAGCAAACCTGGAATCTAAGCAGAATGCTGTGGATGTCTGTAAGGTTGAAACTGGATCAGCTTTCCACGTTCAGGGTGATGGATTTGAGGAACATCCAAGTGATGGTGACTTGTCTGTAAATTTGAGTGATGAGTTTCATAATTTTTCTCCTGCAATATCTTCCAATTCTGAAAAGGTTAACTCTGATGTGACTGACATTAGATACAAAGACAGTGTGGAGTACACCTCTAATTCAAATGATGACTCAAATGTTTGTGTAGAAAGAGAAAGCATGAACTGTAGTATCCTTAAACTTTCCTCTACAAAGGACAATGTAACTACTGGCGAGGGATGTGATACAGATTACGTGGATCCTTTCAGCTCTAAAACTCCTTCGTCTGAGGTCTCTGGTCAGACTTCGTTTGCTGATGCTACTGAAGGCCCCCAATTGGGGAACTTGAGACATTTGCCATCCAAAGAAATTTCTGTTGTTGAGAAGTCAGCCCAACATGAAGTCAATATCACAAGTGCATCTACTAGTGTTGACCCTGAAGTGCAGAAAAATTTGGAAAAAATGTCTCTTACCGAGCTTGATGAAGCCATACCAGAAGGTGGAAACACAGTTGAAGATGATAGTACATTTTTATCTGCATTACACAACACTGGAGATGGCTTCGCATCTGATTTTGCTTCTGATCCTCCTAAGTACATGTTGCCTGCATCAACTGATAGGATTGAGGATGAATTCTCTCTAGGATTGGATTCAGAGACAGTTGGTGTGGAGAAAACTACCTATGATCCATCACTATGGACTCCAATGGAGATAGAAACTGCTACAGGCAATGGGGAGTGCACTGTTTTAGAACATCCTTTGAAGCTCATGAGCACATATACTGAAGTTGAGGGTTGTACTGGGACTAGGGACTCAAATGGAGAGCCCCTGGTGACCAGTTATAATAGGTGTTTCTTCGGGACGGTTGATTACATCTGGCGATCTGAAGGTCTGCAAACTGTCAGGGTCCTTGCTCCAATTCCAAAACATGCTATGCAATGGACTTCAGGATTCCCAACCAGGAAATGGGGTAGCGATCACATTGCCTTGGCTACTGAGTTGGTGTTCATGAAGGGCTCTGACCAATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

801

Amino Acids

89.37

Weight (kDa)

5.39

Isoelectric Point (pI)

48.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 188 - 376 2.5e-08 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 444, 885, 2214
AccB1I GGYRCC 1 cut(s) 1259
AccB7I CCANNNNNTGG 5 cut(s) 677, 960, 1382, 1871, 2337
AccI GTMKAC 1 cut(s) 280
AccII CGCG 1 cut(s) 349
AciI CCGC 8 cut(s) 57, 87, 135, 287, 349, 355, 686, 697
AclWI GGATC 8 cut(s) 303, 1214, 1227, 1342, 1625, 1638, 1949, 2051
AcoI YGGCCR 4 cut(s) 332, 465, 472, 913
AcsI RAATTY 6 cut(s) 538, 956, 1399, 1740, 1822, 2003
AcuI CTGAAG 9 cut(s) 596, 1074, 1077, 1299, 1716, 1830, 2175, 2277, 2301
AcyI GRCGYC 1 cut(s) 216
AfaI GTAC 8 cut(s) 152, 183, 638, 1003, 1502, 1897, 1967, 2170
AflII CTTAAG 1 cut(s) 710
AflIII ACRYGT 1 cut(s) 1968
AgsI TTSAA 8 cut(s) 26, 742, 997, 1160, 1328, 1515, 1886, 2134
AhlI ACTAGT 1 cut(s) 1796
AjnI CCWGG 6 cut(s) 889, 915, 930, 1287, 2199, 2330
AjuI GAANNNNNNNTTGG 4 cut(s) 185, 217, 894, 926
AloI GAACNNNNNNTCC 2 cut(s) 652, 684
Alw21I GWGCWC 4 cut(s) 148, 930, 2114, 2147
Alw26I GTCTC 5 cut(s) 156, 1669, 1714, 1842, 2021
Alw44I GTGCAC 1 cut(s) 2110
AlwI GGATC 8 cut(s) 303, 1214, 1227, 1342, 1625, 1638, 1949, 2051
AlwNI CAGNNNCTG 1 cut(s) 1231
Ama87I CYCGRG 1 cut(s) 630
AoxI GGCC 8 cut(s) 314, 332, 465, 472, 626, 913, 1102, 1699
ApaLI GTGCAC 1 cut(s) 2110
ApeKI GCWGC 3 cut(s) 352, 715, 821
ApoI RAATTY 6 cut(s) 538, 956, 1399, 1740, 1822, 2003
AspA2I CCTAGG 1 cut(s) 1109
AspLEI GCGC 1 cut(s) 9
AspS9I GGNCC 3 cut(s) 1103, 1700, 2278
AsuC2I CCSGG 2 cut(s) 240, 505
AsuHPI GGTGA 8 cut(s) 148, 220, 239, 831, 1158, 1367, 1397, 2215
AvaI CYCGRG 1 cut(s) 630
AvaII GGWCC 1 cut(s) 2278
AvrII CCTAGG 1 cut(s) 1109
BaeGI GKGCMC 1 cut(s) 2114
BaeI ACNNNNGTAYC 2 cut(s) 1546, 1579
BalI TGGCCA 3 cut(s) 467, 474, 915
BamHI GGATCC 2 cut(s) 1219, 1630
BanI GGYRCC 1 cut(s) 1259
BanII GRGCYC 3 cut(s) 148, 2199, 2390
BarI GAAGNNNNNNTAC 2 cut(s) 95, 127
BbsI GAAGAC 2 cut(s) 57, 746
Bbv12I GWGCWC 4 cut(s) 148, 930, 2114, 2147
BbvCI CCTCAGC 1 cut(s) 339
BbvI GCAGC 3 cut(s) 364, 702, 833
BccI CCATC 7 cut(s) 508, 671, 1352, 1376, 1738, 1922, 2068
BceAI ACGGC 2 cut(s) 274, 352
BcgI CGANNNNNNTGC 2 cut(s) 2336, 2370
BciT130I CCWGG 6 cut(s) 891, 917, 932, 1289, 2201, 2332
BciVI GTATCC 1 cut(s) 1574
BcnI CCSGG 2 cut(s) 240, 505
BcoDI GTCTC 5 cut(s) 156, 1669, 1714, 1842, 2021
BcuI ACTAGT 1 cut(s) 1796
BfaI CTAG 5 cut(s) 1110, 1146, 1797, 2012, 2178
BfmI CTRYAG 2 cut(s) 1558, 2094
BfoI RGCGCY 1 cut(s) 10
BfrI CTTAAG 1 cut(s) 710
BfuI GTATCC 1 cut(s) 1574
BisI GCNGC 6 cut(s) 136, 350, 353, 356, 716, 822
BlnI CCTAGG 1 cut(s) 1109
BlsI GCNGC 6 cut(s) 137, 351, 354, 357, 717, 823
Bme1390I CCNGG 8 cut(s) 240, 505, 891, 917, 932, 1289, 2201, 2332
Bme18I GGWCC 1 cut(s) 2278
BmeT110I CYCGRG 1 cut(s) 630
BmgT120I GGNCC 3 cut(s) 1103, 1700, 2278
BmiI GGNNCC 6 cut(s) 95, 1221, 1261, 1632, 1702, 2279
BmrFI CCNGG 8 cut(s) 240, 505, 891, 917, 932, 1289, 2201, 2332
BmrI ACTGGG 1 cut(s) 2181
BmsI GCATC 8 cut(s) 367, 645, 958, 1264, 1678, 1799, 1946, 1988
BmuI ACTGGG 1 cut(s) 2181
BpiI GAAGAC 2 cut(s) 57, 746
BplI GAGNNNNNCTC 2 cut(s) 1490, 1522
BpmI CTGGAG 2 cut(s) 806, 1944
Bpu10I CCTNAGC 1 cut(s) 339
BpuEI CTTGAG 1 cut(s) 1738
BpuMI CCSGG 2 cut(s) 240, 505
BsaAI YACGTR 1 cut(s) 1627
BsaHI GRCGYC 1 cut(s) 216
BsaI GGTCTC 1 cut(s) 1669
BsaJI CCNNGG 7 cut(s) 503, 629, 807, 1109, 1215, 2199, 2358
BsaWI WCCGGW 3 cut(s) 158, 174, 477
Bse118I RCCGGY 2 cut(s) 312, 1262
Bse1I ACTGG 9 cut(s) 456, 524, 667, 671, 1336, 1606, 1927, 2176, 2208
Bse3DI GCAATG 5 cut(s) 66, 530, 2107, 2315, 2352
BseBI CCWGG 6 cut(s) 891, 917, 932, 1289, 2201, 2332
BseDI CCNNGG 7 cut(s) 503, 629, 807, 1109, 1215, 2199, 2358
BseGI GGATG 7 cut(s) 171, 1318, 1372, 1616, 1730, 2005, 2125
BseMI GCAATG 5 cut(s) 66, 530, 2107, 2315, 2352
BseMII CTCAG 6 cut(s) 161, 353, 411, 785, 1650, 2358
BseNI ACTGG 9 cut(s) 456, 524, 667, 671, 1336, 1606, 1927, 2176, 2208
BseRI GAGGAG 1 cut(s) 912
BseSI GKGCMC 1 cut(s) 2114
BseXI GCAGC 3 cut(s) 364, 702, 833
BsgI GTGCAG 1 cut(s) 1835
Bsh1236I CGCG 1 cut(s) 349
Bsh1285I CGRYCG 1 cut(s) 179
BshFI GGCC 8 cut(s) 316, 334, 467, 474, 628, 915, 1104, 1701
BshNI GGYRCC 1 cut(s) 1259
BsiEI CGRYCG 1 cut(s) 179
BsiHKAI GWGCWC 4 cut(s) 148, 930, 2114, 2147
BsiHKCI CYCGRG 1 cut(s) 630
BslFI GGGAC 3 cut(s) 2188, 2195, 2245
BsmAI GTCTC 5 cut(s) 156, 1669, 1714, 1842, 2021
BsmFI GGGAC 3 cut(s) 2188, 2195, 2245
BsmI GAATGC 1 cut(s) 1309
BsnI GGCC 8 cut(s) 316, 334, 467, 474, 628, 915, 1104, 1701
Bso31I GGTCTC 1 cut(s) 1669
BsoBI CYCGRG 1 cut(s) 630
Bsp1286I GDGCHC 6 cut(s) 148, 930, 2114, 2147, 2199, 2390
Bsp143I GATC 8 cut(s) 295, 1219, 1334, 1630, 1954, 2056, 2252, 2347
BspACI CCGC 8 cut(s) 57, 87, 135, 287, 349, 355, 686, 697
BspANI GGCC 8 cut(s) 316, 334, 467, 474, 628, 915, 1104, 1701
BspCNI CTCAG 6 cut(s) 160, 352, 412, 784, 1651, 2359
BspFNI CGCG 1 cut(s) 349
BspHI TCATGA 3 cut(s) 721, 2139, 2379
BspLI GGNNCC 6 cut(s) 95, 1221, 1261, 1632, 1702, 2279
BspPI GGATC 8 cut(s) 303, 1214, 1227, 1342, 1625, 1638, 1949, 2051
BspT107I GGYRCC 1 cut(s) 1259
BspTI CTTAAG 1 cut(s) 710
BspTNI GGTCTC 1 cut(s) 1669
BsrDI GCAATG 5 cut(s) 66, 530, 2107, 2315, 2352
BsrFI RCCGGY 2 cut(s) 312, 1262
BsrI ACTGG 9 cut(s) 456, 524, 667, 671, 1336, 1606, 1927, 2176, 2208
BssAI RCCGGY 2 cut(s) 312, 1262
BssECI CCNNGG 7 cut(s) 503, 629, 807, 1109, 1215, 2199, 2358
BssMI GATC 8 cut(s) 295, 1219, 1334, 1630, 1954, 2056, 2252, 2347
BssNI GRCGYC 1 cut(s) 216
BssT1I CCWWGG 3 cut(s) 807, 1109, 2358
Bst2UI CCWGG 6 cut(s) 891, 917, 932, 1289, 2201, 2332
Bst4CI ACNGT 9 cut(s) 251, 641, 1493, 1559, 1882, 2032, 2116, 2236, 2272
Bst6I CTCTTC 2 cut(s) 27, 98
BstACI GRCGYC 1 cut(s) 216
BstAFI CTTAAG 1 cut(s) 710
BstBAI YACGTR 1 cut(s) 1627
BstC8I GCNNGC 3 cut(s) 1086, 1143, 1977
BstDEI CTNAG 8 cut(s) 147, 339, 420, 771, 1296, 1659, 1962, 2367
BstEII GGTNACC 1 cut(s) 2203
BstENI CCTNNNNNAGG 2 cut(s) 894, 935
BstF5I GGATG 7 cut(s) 171, 1318, 1372, 1616, 1730, 2005, 2125
BstFNI CGCG 1 cut(s) 349
BstH2I RGCGCY 1 cut(s) 10
BstHHI GCGC 1 cut(s) 9
BstKTI GATC 8 cut(s) 298, 1222, 1337, 1633, 1957, 2059, 2255, 2350
BstMAI GTCTC 5 cut(s) 156, 1669, 1714, 1842, 2021
BstMBI GATC 8 cut(s) 295, 1219, 1334, 1630, 1954, 2056, 2252, 2347
BstMCI CGRYCG 1 cut(s) 179
BstMWI GCNNNNNNNGC 8 cut(s) 50, 322, 331, 340, 349, 355, 364, 715
BstNI CCWGG 6 cut(s) 891, 917, 932, 1289, 2201, 2332
BstNSI RCATGY 3 cut(s) 1145, 1972, 2303
BstPI GGTNACC 1 cut(s) 2203
BstSCI CCNGG 8 cut(s) 238, 503, 889, 915, 930, 1287, 2199, 2330
BstSFI CTRYAG 2 cut(s) 1558, 2094
BstSLI GKGCMC 1 cut(s) 2114
BstUI CGCG 1 cut(s) 349
BstV1I GCAGC 3 cut(s) 364, 702, 833
BstV2I GAAGAC 2 cut(s) 57, 746
BstX2I RGATCY 2 cut(s) 1219, 1630
BstXI CCANNNNNNTGG 1 cut(s) 2067
BstYI RGATCY 2 cut(s) 1219, 1630
BsuI GTATCC 1 cut(s) 1574
BsuRI GGCC 8 cut(s) 316, 334, 467, 474, 628, 915, 1104, 1701
BtsCI GGATG 7 cut(s) 171, 1318, 1372, 1616, 1730, 2005, 2125
BtsI GCAGTG 2 cut(s) 404, 1255
BtsIMutI CAGTG 6 cut(s) 404, 646, 1255, 1498, 1920, 2112
Cac8I GCNNGC 3 cut(s) 1086, 1143, 1977
CaiI CAGNNNCTG 1 cut(s) 1231
CciI TCATGA 3 cut(s) 721, 2139, 2379
CfoI GCGC 1 cut(s) 9
Cfr10I RCCGGY 2 cut(s) 312, 1262
Cfr13I GGNCC 3 cut(s) 1103, 1700, 2278
CseI GACGC 2 cut(s) 224, 785
Csp6I GTAC 8 cut(s) 151, 182, 637, 1002, 1501, 1896, 1966, 2169
CviQI GTAC 8 cut(s) 151, 182, 637, 1002, 1501, 1896, 1966, 2169
DdeI CTNAG 8 cut(s) 147, 339, 420, 771, 1296, 1659, 1962, 2367
DpnI GATC 8 cut(s) 297, 1221, 1336, 1632, 1956, 2058, 2254, 2349
DpnII GATC 8 cut(s) 295, 1219, 1334, 1630, 1954, 2056, 2252, 2347
EaeI YGGCCR 4 cut(s) 332, 465, 472, 913
Eam1104I CTCTTC 2 cut(s) 27, 98
EarI CTCTTC 2 cut(s) 27, 98
EciI GGCGGA 2 cut(s) 102, 276
Ecl136II GAGCTC 1 cut(s) 146
Eco130I CCWWGG 3 cut(s) 807, 1109, 2358
Eco147I AGGCCT 1 cut(s) 628
Eco24I GRGCYC 3 cut(s) 148, 2199, 2390
Eco31I GGTCTC 1 cut(s) 1669
Eco47I GGWCC 1 cut(s) 2278
Eco53kI GAGCTC 1 cut(s) 146
Eco57I CTGAAG 9 cut(s) 596, 1074, 1077, 1299, 1716, 1830, 2175, 2277, 2301
Eco88I CYCGRG 1 cut(s) 630
Eco91I GGTNACC 1 cut(s) 2203
EcoICRI GAGCTC 1 cut(s) 146
EcoNI CCTNNNNNAGG 2 cut(s) 894, 935
EcoO109I RGGNCCY 2 cut(s) 1700, 2278
EcoO65I GGTNACC 1 cut(s) 2203
EcoRI GAATTC 1 cut(s) 2003
EcoRII CCWGG 6 cut(s) 889, 915, 930, 1287, 2199, 2330
EcoT14I CCWWGG 3 cut(s) 807, 1109, 2358
EcoT38I GRGCYC 3 cut(s) 148, 2199, 2390
ErhI CCWWGG 3 cut(s) 807, 1109, 2358
FaqI GGGAC 3 cut(s) 2188, 2195, 2245
FauNDI CATATG 2 cut(s) 510, 647
FblI GTMKAC 1 cut(s) 280
Fnu4HI GCNGC 6 cut(s) 136, 350, 353, 356, 716, 822
FokI GGATG 7 cut(s) 158, 1325, 1359, 1623, 1717, 2012, 2112
FriOI GRGCYC 3 cut(s) 148, 2199, 2390
Fsp4HI GCNGC 6 cut(s) 136, 350, 353, 356, 716, 822
FspBI CTAG 5 cut(s) 1110, 1146, 1797, 2012, 2178
GlaI GCGC 1 cut(s) 8
GluI GCNGC 6 cut(s) 136, 350, 353, 356, 716, 822
GsuI CTGGAG 2 cut(s) 806, 1944
HaeII RGCGCY 1 cut(s) 10
HaeIII GGCC 8 cut(s) 316, 334, 467, 474, 628, 915, 1104, 1701
HgaI GACGC 2 cut(s) 224, 785
HhaI GCGC 1 cut(s) 9
Hin1I GRCGYC 1 cut(s) 216
Hin6I GCGC 1 cut(s) 7
HinP1I GCGC 1 cut(s) 7
HincII GTYRAC 3 cut(s) 281, 1459, 1804
HindII GTYRAC 3 cut(s) 281, 1459, 1804
HindIII AAGCTT 5 cut(s) 223, 485, 746, 908, 1086
HpaI GTTAAC 1 cut(s) 1459
HphI GGTGA 8 cut(s) 148, 220, 239, 831, 1158, 1367, 1397, 2215
Hpy166II GTNNAC 7 cut(s) 281, 1063, 1166, 1459, 1503, 1804, 2112
Hpy8I GTNNAC 7 cut(s) 281, 1063, 1166, 1459, 1503, 1804, 2112
Hpy99I CGWCG 5 cut(s) 35, 38, 218, 282, 295
HpyAV CCTTC 7 cut(s) 27, 1177, 1662, 1691, 1862, 2252, 2377
HpyCH4III ACNGT 9 cut(s) 251, 641, 1493, 1559, 1882, 2032, 2116, 2236, 2272
HpyCH4IV ACGT 4 cut(s) 30, 153, 1347, 1626
HpyCH4V TGCA 9 cut(s) 821, 1433, 1790, 1816, 1910, 1979, 2112, 2266, 2308
HpyF10VI GCNNNNNNNGC 8 cut(s) 50, 322, 331, 340, 349, 355, 364, 715
HpyF3I CTNAG 8 cut(s) 147, 339, 420, 771, 1296, 1659, 1962, 2367
HpySE526I ACGT 4 cut(s) 30, 153, 1347, 1626
Hsp92I GRCGYC 1 cut(s) 216
HspAI GCGC 1 cut(s) 7
KspAI GTTAAC 1 cut(s) 1459
Kzo9I GATC 8 cut(s) 295, 1219, 1334, 1630, 1954, 2056, 2252, 2347
LmnI GCTCC 3 cut(s) 933, 976, 2290
Lsp1109I GCAGC 3 cut(s) 364, 702, 833
LweI GCATC 8 cut(s) 367, 645, 958, 1264, 1678, 1799, 1946, 1988
MaeI CTAG 5 cut(s) 1110, 1146, 1797, 2012, 2178
MaeII ACGT 4 cut(s) 30, 153, 1347, 1626
MaeIII GTNAC 6 cut(s) 154, 866, 1385, 1468, 1594, 2203
MalI GATC 8 cut(s) 297, 1221, 1336, 1632, 1956, 2058, 2254, 2349
MboI GATC 8 cut(s) 295, 1219, 1334, 1630, 1954, 2056, 2252, 2347
MfeI CAATTG 2 cut(s) 997, 1706
MflI RGATCY 2 cut(s) 1219, 1630
MhlI GDGCHC 6 cut(s) 148, 930, 2114, 2147, 2199, 2390
MlsI TGGCCA 3 cut(s) 467, 474, 915
MluNI TGGCCA 3 cut(s) 467, 474, 915
MlyI GAGTC 6 cut(s) 158, 797, 1021, 1517, 2065, 2177
MmeI TCCRAC 2 cut(s) 273, 351
Mox20I TGGCCA 3 cut(s) 467, 474, 915
MscI TGGCCA 3 cut(s) 467, 474, 915
MseI TTAA 6 cut(s) 711, 906, 1119, 1211, 1458, 1569
MslI CAYNNNNRTG 4 cut(s) 59, 1199, 1377, 2065
Msp20I TGGCCA 3 cut(s) 467, 474, 915
MspA1I CMGCKG 3 cut(s) 355, 569, 1094
MspCI CTTAAG 1 cut(s) 710
MspR9I CCNGG 8 cut(s) 240, 505, 891, 917, 932, 1289, 2201, 2332
MunI CAATTG 2 cut(s) 997, 1706
Mva1269I GAATGC 1 cut(s) 1309
MvaI CCWGG 6 cut(s) 891, 917, 932, 1289, 2201, 2332
MvnI CGCG 1 cut(s) 349
MwoI GCNNNNNNNGC 8 cut(s) 50, 322, 331, 340, 349, 355, 364, 715
NciI CCSGG 2 cut(s) 240, 505
NdeI CATATG 2 cut(s) 510, 647
NdeII GATC 8 cut(s) 295, 1219, 1334, 1630, 1954, 2056, 2252, 2347
NlaIV GGNNCC 6 cut(s) 95, 1221, 1261, 1632, 1702, 2279
NmuCI GTSAC 4 cut(s) 154, 1385, 1468, 2203
NspI RCATGY 3 cut(s) 1145, 1972, 2303
PaeI GCATGC 1 cut(s) 1145
PagI TCATGA 3 cut(s) 721, 2139, 2379
PceI AGGCCT 1 cut(s) 628
PciI ACATGT 1 cut(s) 1968
PcsI WCGNNNNNNNCGW 1 cut(s) 122
PctI GAATGC 1 cut(s) 1309
PfeI GAWTC 6 cut(s) 118, 201, 845, 1292, 2021, 2322
PflMI CCANNNNNTGG 5 cut(s) 677, 960, 1382, 1871, 2337
PfoI TCCNGGA 2 cut(s) 889, 930
PkrI GCNGC 6 cut(s) 137, 351, 354, 357, 717, 823
PleI GAGTC 6 cut(s) 158, 796, 1020, 1517, 2065, 2177
PpsI GAGTC 6 cut(s) 158, 796, 1020, 1517, 2065, 2177
Ppu21I YACGTR 1 cut(s) 1627
PpuMI RGGWCCY 1 cut(s) 2278
PscI ACATGT 1 cut(s) 1968
PsiI TTATAA 3 cut(s) 444, 885, 2214
Psp124BI GAGCTC 1 cut(s) 148
Psp5II RGGWCCY 1 cut(s) 2278
Psp6I CCWGG 6 cut(s) 889, 915, 930, 1287, 2199, 2330
PspEI GGTNACC 1 cut(s) 2203
PspGI CCWGG 6 cut(s) 889, 915, 930, 1287, 2199, 2330
PspN4I GGNNCC 6 cut(s) 95, 1221, 1261, 1632, 1702, 2279
PspPI GGNCC 3 cut(s) 1103, 1700, 2278
PspPPI RGGWCCY 1 cut(s) 2278
PstNI CAGNNNCTG 1 cut(s) 1231
PsuI RGATCY 2 cut(s) 1219, 1630
PvuII CAGCTG 2 cut(s) 569, 1094
RsaI GTAC 8 cut(s) 152, 183, 638, 1003, 1502, 1897, 1967, 2170
RsaNI GTAC 8 cut(s) 151, 182, 637, 1002, 1501, 1896, 1966, 2169
RseI CAYNNNNRTG 4 cut(s) 59, 1199, 1377, 2065
SacI GAGCTC 1 cut(s) 148
SalI GTCGAC 1 cut(s) 279
SaqAI TTAA 6 cut(s) 711, 906, 1119, 1211, 1458, 1569
SatI GCNGC 6 cut(s) 136, 350, 353, 356, 716, 822
Sau3AI GATC 8 cut(s) 295, 1219, 1334, 1630, 1954, 2056, 2252, 2347
Sau96I GGNCC 3 cut(s) 1103, 1700, 2278
SchI GAGTC 6 cut(s) 158, 797, 1021, 1517, 2065, 2177
ScrFI CCNGG 8 cut(s) 240, 505, 891, 917, 932, 1289, 2201, 2332
SduI GDGCHC 6 cut(s) 148, 930, 2114, 2147, 2199, 2390
SfaNI GCATC 8 cut(s) 367, 645, 958, 1264, 1678, 1799, 1946, 1988
SfcI CTRYAG 2 cut(s) 1558, 2094
SinI GGWCC 1 cut(s) 2278
SmiMI CAYNNNNRTG 4 cut(s) 59, 1199, 1377, 2065
SmlI CTYRAG 2 cut(s) 710, 1717
SmoI CTYRAG 2 cut(s) 710, 1717
SpeI ACTAGT 1 cut(s) 1796
SphI GCATGC 1 cut(s) 1145
SseBI AGGCCT 1 cut(s) 628
SsiI CCGC 8 cut(s) 57, 87, 135, 287, 349, 355, 686, 697
SspI AATATT 1 cut(s) 766
SspMI CTAG 5 cut(s) 1110, 1146, 1797, 2012, 2178
SstI GAGCTC 1 cut(s) 148
StuI AGGCCT 1 cut(s) 628
StyD4I CCNGG 8 cut(s) 238, 503, 889, 915, 930, 1287, 2199, 2330
StyI CCWWGG 3 cut(s) 807, 1109, 2358
TaaI ACNGT 9 cut(s) 251, 641, 1493, 1559, 1882, 2032, 2116, 2236, 2272
TaiI ACGT 4 cut(s) 33, 156, 1350, 1629
TaqI TCGA 5 cut(s) 82, 116, 280, 379, 549
TatI WGTACW 5 cut(s) 1001, 1500, 1895, 1965, 2168
TauI GCSGC 3 cut(s) 138, 352, 358
TfiI GAWTC 6 cut(s) 118, 201, 845, 1292, 2021, 2322
Tru1I TTAA 6 cut(s) 711, 906, 1119, 1211, 1458, 1569
Tru9I TTAA 6 cut(s) 711, 906, 1119, 1211, 1458, 1569
TscAI CASTG 6 cut(s) 411, 646, 1255, 1498, 1927, 2119
TseFI GTSAC 4 cut(s) 154, 1385, 1468, 2203
TseI GCWGC 3 cut(s) 352, 715, 821
Tsp45I GTSAC 4 cut(s) 154, 1385, 1468, 2203
TspGWI ACGGA 4 cut(s) 106, 114, 243, 266
TspRI CASTG 6 cut(s) 411, 646, 1255, 1498, 1927, 2119
Van91I CCANNNNNTGG 5 cut(s) 677, 960, 1382, 1871, 2337
Vha464I CTTAAG 1 cut(s) 710
VneI GTGCAC 1 cut(s) 2110
VpaK11BI GGWCC 1 cut(s) 2278
XagI CCTNNNNNAGG 2 cut(s) 894, 935
XapI RAATTY 6 cut(s) 538, 956, 1399, 1740, 1822, 2003
XceI RCATGY 3 cut(s) 1145, 1972, 2303
XcmI CCANNNNNNNNNTGG 2 cut(s) 1176, 2334
XmaJI CCTAGG 1 cut(s) 1109
XmiI GTMKAC 1 cut(s) 280
XspI CTAG 5 cut(s) 1110, 1146, 1797, 2012, 2178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.