Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0007645

Physical Location & Seq

Genomic Coordinates Reverse (-)

9676618 .. 9680850

Gene Structure 4233 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1133500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2280 bp

ATGGTGGAAAAAGGCTGTGGCGGAGGACATGGAGAGGCGGAGAGCTGCGGAGGCCGCTCGGTTGTGGTGGGGGTGAAGCTGGACTCGAAGAGTAGAGAGCTGCTGACATGGGCTCTGGTTAAGGTTGCTCAGCCTGGTGATCGCGTGATTGCCCTCCATGTGCTCGGTAAAAATGAAATTGTTGATCGAGATGGGAAGTCGTCGCTTCTTTCTCTTGTCAAAGCGTTTGACTCTGTTCTTGCAGTCTATGATGGTTTCTGCAACTTGAAGCAGGTGGATCTCAAGCTTAAAATCTGCAGAGGAACGTCAGTCAAGAAAATTTTAGTCCGGGAAGCGAATTCATATACTGCAAGTAAGGTTATAGTGGGAACTGCCCAGAACCACCATAAAATCCGGTCATCGACCACCGTGGCCAAGTACTGTGCCAAGAAACTGTCTAAGGATTGTGGGGTTCTTGCTGTAAATAATGGGAAGGTTTTGTTTAACAGAGAAGGCTCTCAAACAACTTACTGTGACCCCCAAGGAAGTGAAGAGCATCGCCGGAACAGTTTGCTCAGCGCATTTCACCGATCAGTGTATAAGTCTTCCAAAGTACTAAATGAAGGCAGTGACAGTGTGACCTTAAAGGACACATATGGTCAGGTTAATTGTCAAAACTTGGAGCAGGGATTAGCTAAAGTCTTTCTGGAATGTTCGGAGAGTGTTGAGAAACAGAAATGCTCAGTTTGTTCCCGGCCCTCGGTAGATAGTTCTTGTCACCAATCTGCAGAAGAGTCCTCGGATGACAGTGATAATAAATATATGGCTATAGTGCCAGTGCAGAAAGAAGAGGCACTAGCACCGAGCTCAATTTCTATGTTGATCAAAGAATTACCGGAAGTGAGACCTGGTTGGCCACTGCTTCGACGGGCTGTATTACCAGACCGACAATCTCCAGAGAGGTCTTGGATCCGGAAGATCTCAGTAGTTCAGTGGGCAATGCAGTTGCCCAGTAGACAGCATTCAACTGCCAGCAATTTCGATGAAGGGAAAAATAGTTGTGATTCAGATCAACCTTCCTGTTTGAATGGAGAAAGTGGCGCAATTGTTGCGGTGGGTGGTGAGGCAATGACTGCCCCACCTTCACCGGACTACAATTCAAAAAGCCTGCCTAAAGAATTGGAGGGACTGCATGAAAAGTACTCTGCTACTTGCAGATTGTTCAACTACCAGGAACTTCAGTCAGCTACATCATACTTCTTGGCCGAAAACTTCATCGGGAGAGGCGGTAGCAGTCAAGTTTATAGAGGCTGCCTTCCTGATGGCAAGGAACTCGCTGTGAAAATCTTAAAACCATCCGAAGATGTGTTGAAGGAGTTTGTTTTGGAAATAGAGATCATTACTACCTTAAATCACAAGAACATTATTTCCCTTCTGGGGTTCTGTTTTGAGGATAACAATCTTCTCTTGGTTTATGATTTCTTATCAAGAGGAAGCCTTGAAGAGAATCTCCATGGAACTAAGAAGGATCCCCTTGCATTTGGTTGGAACGAGAGATATAAGGTGGCCGTGGGGGTAGCTGAGGCCTTGGATTATCTGCACACCGGCAGTGCACAACCTGTAATCCACAGGGATGTCAAATCATCCAATATTCTACTATCTGATGATTTTGAGCCGCAGCTCTCTGATTTTGGACTAGCAAAATGGGCATCAACCTCATCTTCGCATATAACATGCACTGATGTTGCAGGCACCTTTGGCTATTTGGCTCCTGAATACTTCATGTATGGCAAAGTAAACGACAAAATTGACGTGTATGCATTTGGTGTTGTACTCCTCGAGCTTCTTTCAGGAAGAAAGCCTATAAGCAGTGACCATCCAAAAGGCCATGAGAGCCTAGTCATGTGGGCCAAGCCAATTCTAAACAGTGGGAAGGTGTCCCAACTATTAGATCCGTGTTTGGGCGATAACTATGACCAGGGCCAGATAGAGAGAATGGTTTTAGCAGCCACTCTTTGTATCAGACATGCTTCACGAGCTAGGCCCCAGATGAGCTTTATCGTGAAGCTTCTTCAAGGCGATGCAGATGTGACTAAGTGGTCCAGGCTGCAAGTTCATGCCATGGAGGAATCTGATGTGCTCGAAAACGAAACGTGTCCACGTTCAAATCTGCAGTCTCACCTTAACCTTGCACTGCTTGATGTGGAGGATGACTCACTCTCCATGAGCAGCATTGAGCAAACTGTCTCCTTAGAGGACTACCTGAAAGGGCGGTGGAGCCGCTCGTCGAGCTTCGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009611 GO:0009620 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Usp

Protein Analysis

760

Amino Acids

83.59

Weight (kDa)

6.07

Isoelectric Point (pI)

47.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	416 - 622	4.7e-42	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	417 - 618	7.3e-38	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007645)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G77280 AT1G77280
fragaria_vesca	FvH4_2g27940 FvH4_2g27940 FvH4_2g27940 FvH4_2g27940
malus_domestica	MD08G1160300.v1.1 MD08G1160400.v1.1 MD15G1133500.v1.1
prunus_persica	Prupe.1G489100_v2.0.a1 Prupe.1G489100_v2.0.a1
pyrus_communis	pycom08g13520 pycom15g12030
rosa_chinensis	RchiOBHm_Chr6g0296761
rosa_laevigata	RLG00000011661
rosa_multiflora	Rmu_ssc0000192.1_g000010
rosa_roxburghii	Rroxscaffold_7G00171190
rosa_rugosa	Rorug06G0265700 Rorug06G0265800
rosa_samantha	Rh6AG376800 Rh6BG385100 Rh6CG390400 Rh6DG377500
rosa_wichuraiana	Rw6G032940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	262
AasI	GACNNNNNNGTC	1 cut(s)	2077
Acc36I	ACCTGC	1 cut(s)	262
AccB1I	GGYRCC	1 cut(s)	1730
AccBSI	CCGCTC	2 cut(s)	57, 2262
AccI	GTMKAC	1 cut(s)	994
AccII	CGCG	1 cut(s)	144
AccIII	TCCGGA	1 cut(s)	951
AciI	CCGC	9 cut(s)	21, 38, 48, 55, 1091, 1266, 1655, 2251, 2260
AclWI	GGATC	6 cut(s)	285, 943, 956, 1502, 1515, 1925
AcoI	YGGCCR	4 cut(s)	411, 893, 1242, 1545
AcsI	RAATTY	2 cut(s)	318, 337
AcuI	CTGAAG	1 cut(s)	1202
AfaI	GTAC	4 cut(s)	419, 594, 1181, 1812
AfiI	CCNNNNNNNGG	3 cut(s)	739, 1416, 1940
AflIII	ACRYGT	2 cut(s)	1791, 2132
AgsI	TTSAA	9 cut(s)	268, 1005, 1066, 1140, 1204, 1351, 1481, 2054, 2145
AjiI	CACGTC	1 cut(s)	1792
AjnI	CCWGG	5 cut(s)	133, 886, 1209, 1956, 2081
AloI	GAACNNNNNNTCC	2 cut(s)	1391, 1423
Alw21I	GWGCWC	4 cut(s)	165, 848, 1594, 2121
Alw26I	GTCTC	3 cut(s)	877, 2160, 2230
Alw44I	GTGCAC	1 cut(s)	1590
AlwI	GGATC	6 cut(s)	285, 943, 956, 1502, 1515, 1925
Ama87I	CYCGRG	1 cut(s)	1817
Aor13HI	TCCGGA	1 cut(s)	951
ApaLI	GTGCAC	1 cut(s)	1590
ApeKI	GCWGC	7 cut(s)	45, 100, 1290, 1657, 1985, 2086, 2208
ApoI	RAATTY	2 cut(s)	318, 337
ArsI	GACNNNNNNTTYG	2 cut(s)	2138, 2170
AspLEI	GCGC	2 cut(s)	560, 1082
AspS9I	GGNCC	5 cut(s)	735, 1887, 1960, 2022, 2079
AsuC2I	CCSGG	2 cut(s)	329, 733
AsuHPI	GGTGA	7 cut(s)	85, 149, 557, 749, 1112, 1116, 2150
AvaI	CYCGRG	1 cut(s)	1817
AvaII	GGWCC	1 cut(s)	2079
BaeGI	GKGCMC	1 cut(s)	1594
BalI	TGGCCA	2 cut(s)	413, 895
BamHI	GGATCC	2 cut(s)	948, 1507
BanI	GGYRCC	1 cut(s)	1730
BanII	GRGCYC	2 cut(s)	115, 848
BauI	CACGAG	1 cut(s)	2013
BbsI	GAAGAC	1 cut(s)	576
Bbv12I	GWGCWC	4 cut(s)	165, 848, 1594, 2121
BbvCI	CCTCAGC	1 cut(s)	1560
BbvI	GCAGC	7 cut(s)	32, 87, 1277, 1669, 1997, 2073, 2220
BccI	CCATC	5 cut(s)	185, 245, 1295, 1342, 1863
BceAI	ACGGC	1 cut(s)	1532
BcgI	CGANNNNNNTGC	2 cut(s)	1294, 1328
BciT130I	CCWGG	5 cut(s)	135, 888, 1211, 1958, 2083
BclI	TGATCA	1 cut(s)	861
BcnI	CCSGG	2 cut(s)	329, 733
BcoDI	GTCTC	3 cut(s)	877, 2160, 2230
BfaI	CTAG	4 cut(s)	836, 1676, 1877, 2019
BfmI	CTRYAG	4 cut(s)	295, 765, 807, 2150
BfuAI	ACCTGC	1 cut(s)	262
BglII	AGATCT	1 cut(s)	957
BlpI	GCTNAGC	2 cut(s)	129, 554
BmcAI	AGTACT	3 cut(s)	419, 594, 1181
Bme1390I	CCNGG	7 cut(s)	135, 329, 733, 888, 1211, 1958, 2083
Bme18I	GGWCC	1 cut(s)	2079
BmeT110I	CYCGRG	1 cut(s)	1817
BmgBI	CACGTC	1 cut(s)	1792
BmgT120I	GGNCC	5 cut(s)	735, 1887, 1960, 2022, 2079
BmiI	GGNNCC	6 cut(s)	950, 1509, 1732, 1749, 2024, 2258
BmrFI	CCNGG	7 cut(s)	135, 329, 733, 888, 1211, 1958, 2083
BmrI	ACTGGG	1 cut(s)	984
BmsI	GCATC	3 cut(s)	544, 1697, 2050
BmuI	ACTGGG	1 cut(s)	984
BpiI	GAAGAC	1 cut(s)	576
BplI	GAGNNNNNCTC	2 cut(s)	2177, 2209
BpmI	CTGGAG	1 cut(s)	918
Bpu10I	CCTNAGC	1 cut(s)	1560
Bpu1102I	GCTNAGC	2 cut(s)	129, 554
BpuEI	CTTGAG	1 cut(s)	266
BpuMI	CCSGG	2 cut(s)	329, 733
BsaBI	GATNNNNATC	2 cut(s)	1047, 1437
BsaI	GGTCTC	1 cut(s)	877
BsaJI	CCNNGG	9 cut(s)	408, 520, 738, 777, 1492, 1548, 1566, 1957, 2100
BsaWI	WCCGGW	4 cut(s)	393, 874, 951, 1126
BsaXI	ACNNNNNCTCC	4 cut(s)	1252, 1282, 2183, 2213
Bsc4I	CCNNNNNNNGG	3 cut(s)	739, 1416, 1940
Bse118I	RCCGGY	1 cut(s)	1583
Bse1I	ACTGG	2 cut(s)	815, 990
Bse3DI	GCAATG	2 cut(s)	984, 1113
Bse8I	GATNNNNATC	2 cut(s)	1047, 1437
BseAI	TCCGGA	1 cut(s)	951
BseBI	CCWGG	5 cut(s)	135, 888, 1211, 1958, 2083
BseDI	CCNNGG	9 cut(s)	408, 520, 738, 777, 1492, 1548, 1566, 1957, 2100
BseGI	GGATG	6 cut(s)	787, 1334, 1618, 1622, 1855, 2194
BseJI	GATNNNNATC	2 cut(s)	1047, 1437
BseLI	CCNNNNNNNGG	3 cut(s)	739, 1416, 1940
BseMI	GCAATG	2 cut(s)	984, 1113
BseMII	CTCAG	5 cut(s)	143, 568, 735, 975, 1551
BseNI	ACTGG	2 cut(s)	815, 990
BseRI	GAGGAG	1 cut(s)	1805
BseSI	GKGCMC	1 cut(s)	1594
BseXI	GCAGC	7 cut(s)	32, 87, 1277, 1669, 1997, 2073, 2220
BsgI	GTGCAG	2 cut(s)	839, 1562
Bsh1236I	CGCG	1 cut(s)	144
BshNI	GGYRCC	1 cut(s)	1730
BsiHKAI	GWGCWC	4 cut(s)	165, 848, 1594, 2121
BsiHKCI	CYCGRG	1 cut(s)	1817
BsiSI	CCGG	8 cut(s)	328, 394, 541, 733, 875, 952, 1127, 1584
BslFI	GGGAC	2 cut(s)	1179, 1903
BslI	CCNNNNNNNGG	3 cut(s)	739, 1416, 1940
BsmAI	GTCTC	3 cut(s)	877, 2160, 2230
BsmFI	GGGAC	2 cut(s)	1179, 1903
BsmI	GAATGC	1 cut(s)	1000
Bso31I	GGTCTC	1 cut(s)	877
BsoBI	CYCGRG	1 cut(s)	1817
Bsp1286I	GDGCHC	5 cut(s)	115, 165, 848, 1594, 2121
Bsp13I	TCCGGA	1 cut(s)	951
Bsp1720I	GCTNAGC	2 cut(s)	129, 554
Bsp19I	CCATGG	2 cut(s)	1492, 2100
BspACI	CCGC	9 cut(s)	21, 38, 48, 55, 1091, 1266, 1655, 2251, 2260
BspCNI	CTCAG	5 cut(s)	142, 567, 734, 974, 1552
BspEI	TCCGGA	1 cut(s)	951
BspFNI	CGCG	1 cut(s)	144
BspLI	GGNNCC	6 cut(s)	950, 1509, 1732, 1749, 2024, 2258
BspMAI	CTGCAG	3 cut(s)	299, 769, 2154
BspMI	ACCTGC	1 cut(s)	262
BspPI	GGATC	6 cut(s)	285, 943, 956, 1502, 1515, 1925
BspQI	GCTCTTC	1 cut(s)	525
BspT107I	GGYRCC	1 cut(s)	1730
BspTNI	GGTCTC	1 cut(s)	877
BsrBI	CCGCTC	2 cut(s)	57, 2262
BsrDI	GCAATG	2 cut(s)	984, 1113
BsrFI	RCCGGY	1 cut(s)	1583
BsrI	ACTGG	2 cut(s)	815, 990
BssAI	RCCGGY	1 cut(s)	1583
BssECI	CCNNGG	9 cut(s)	408, 520, 738, 777, 1492, 1548, 1566, 1957, 2100
BssSI	CACGAG	1 cut(s)	2013
BssT1I	CCWWGG	4 cut(s)	520, 1492, 1566, 2100
Bst2BI	CACGAG	1 cut(s)	2013
Bst2UI	CCWGG	5 cut(s)	135, 888, 1211, 1958, 2083
Bst4CI	ACNGT	9 cut(s)	409, 422, 435, 512, 548, 614, 788, 1907, 2224
Bst6I	CTCTTC	5 cut(s)	83, 525, 765, 822, 1476
BstAPI	GCANNNNNTGC	2 cut(s)	1088, 1112
BstC8I	GCNNGC	3 cut(s)	1012, 1148, 1729
BstDEI	CTNAG	9 cut(s)	129, 438, 554, 721, 961, 1500, 1560, 2073, 2230
BstDSI	CCRYGG	4 cut(s)	408, 1492, 1548, 2100
BstF5I	GGATG	6 cut(s)	787, 1334, 1618, 1622, 1855, 2194
BstFNI	CGCG	1 cut(s)	144
BstHHI	GCGC	2 cut(s)	560, 1082
BstMAI	GTCTC	3 cut(s)	877, 2160, 2230
BstMWI	GCNNNNNNNGC	9 cut(s)	51, 54, 1088, 1112, 1685, 1737, 1872, 2015, 2268
BstNI	CCWGG	5 cut(s)	135, 888, 1211, 1958, 2083
BstNSI	RCATGY	2 cut(s)	1716, 2009
BstSCI	CCNGG	7 cut(s)	133, 327, 731, 886, 1209, 1956, 2081
BstSFI	CTRYAG	4 cut(s)	295, 765, 807, 2150
BstSLI	GKGCMC	1 cut(s)	1594
BstUI	CGCG	1 cut(s)	144
BstV1I	GCAGC	7 cut(s)	32, 87, 1277, 1669, 1997, 2073, 2220
BstV2I	GAAGAC	1 cut(s)	576
BstX2I	RGATCY	5 cut(s)	277, 948, 957, 1507, 1930
BstYI	RGATCY	5 cut(s)	277, 948, 957, 1507, 1930
BtgI	CCRYGG	4 cut(s)	408, 1492, 1548, 2100
BtgZI	GCGATG	2 cut(s)	521, 2073
BtrI	CACGTC	1 cut(s)	1792
BtsCI	GGATG	6 cut(s)	787, 1334, 1618, 1622, 1855, 2194
BtsI	GCAGTG	5 cut(s)	613, 896, 1594, 1855, 2171
BveI	ACCTGC	1 cut(s)	262
Cac8I	GCNNGC	3 cut(s)	1012, 1148, 1729
CfoI	GCGC	2 cut(s)	560, 1082
Cfr10I	RCCGGY	1 cut(s)	1583
Cfr13I	GGNCC	5 cut(s)	735, 1887, 1960, 2022, 2079
CsiI	ACCWGGT	1 cut(s)	886
Csp6I	GTAC	4 cut(s)	418, 593, 1180, 1811
CviQI	GTAC	4 cut(s)	418, 593, 1180, 1811
DdeI	CTNAG	9 cut(s)	129, 438, 554, 721, 961, 1500, 1560, 2073, 2230
DrdI	GACNNNNNNGTC	1 cut(s)	2077
DseDI	GACNNNNNNGTC	1 cut(s)	2077
EaeI	YGGCCR	4 cut(s)	411, 893, 1242, 1545
Eam1104I	CTCTTC	5 cut(s)	83, 525, 765, 822, 1476
EarI	CTCTTC	5 cut(s)	83, 525, 765, 822, 1476
EciI	GGCGGA	2 cut(s)	36, 53
Ecl136II	GAGCTC	1 cut(s)	846
Eco130I	CCWWGG	4 cut(s)	520, 1492, 1566, 2100
Eco147I	AGGCCT	1 cut(s)	1565
Eco24I	GRGCYC	2 cut(s)	115, 848
Eco31I	GGTCTC	1 cut(s)	877
Eco47I	GGWCC	1 cut(s)	2079
Eco53kI	GAGCTC	1 cut(s)	846
Eco57I	CTGAAG	1 cut(s)	1202
Eco88I	CYCGRG	1 cut(s)	1817
EcoICRI	GAGCTC	1 cut(s)	846
EcoO109I	RGGNCCY	1 cut(s)	2022
EcoRI	GAATTC	1 cut(s)	337
EcoRII	CCWGG	5 cut(s)	133, 886, 1209, 1956, 2081
EcoT14I	CCWWGG	4 cut(s)	520, 1492, 1566, 2100
EcoT22I	ATGCAT	1 cut(s)	1801
EcoT38I	GRGCYC	2 cut(s)	115, 848
ErhI	CCWWGG	4 cut(s)	520, 1492, 1566, 2100
FaqI	GGGAC	2 cut(s)	1179, 1903
FauNDI	CATATG	1 cut(s)	634
FbaI	TGATCA	1 cut(s)	861
FblI	GTMKAC	1 cut(s)	994
FokI	GGATG	6 cut(s)	794, 1321, 1609, 1625, 1842, 2201
FriOI	GRGCYC	2 cut(s)	115, 848
FspBI	CTAG	4 cut(s)	836, 1676, 1877, 2019
GlaI	GCGC	2 cut(s)	559, 1081
GsuI	CTGGAG	1 cut(s)	918
HapII	CCGG	8 cut(s)	328, 394, 541, 733, 875, 952, 1127, 1584
HhaI	GCGC	2 cut(s)	560, 1082
Hin6I	GCGC	2 cut(s)	558, 1080
HinP1I	GCGC	2 cut(s)	558, 1080
HindIII	AAGCTT	2 cut(s)	284, 2045
HinfI	GANTC	7 cut(s)	83, 230, 773, 1043, 1486, 2108, 2192
HpaII	CCGG	8 cut(s)	328, 394, 541, 733, 875, 952, 1127, 1584
HphI	GGTGA	7 cut(s)	85, 149, 557, 749, 1112, 1116, 2150
Hpy166II	GTNNAC	4 cut(s)	995, 1592, 1777, 2138
Hpy188I	TCNGA	8 cut(s)	697, 781, 1048, 1339, 1642, 1666, 2003, 2113
Hpy8I	GTNNAC	4 cut(s)	995, 1592, 1777, 2138
Hpy99I	CGWCG	3 cut(s)	205, 909, 2269
HpyCH4III	ACNGT	9 cut(s)	409, 422, 435, 512, 548, 614, 788, 1907, 2224
HpyCH4IV	ACGT	4 cut(s)	305, 1791, 2132, 2140
HpyF10VI	GCNNNNNNNGC	9 cut(s)	51, 54, 1088, 1112, 1685, 1737, 1872, 2015, 2268
HpyF3I	CTNAG	9 cut(s)	129, 438, 554, 721, 961, 1500, 1560, 2073, 2230
HpySE526I	ACGT	4 cut(s)	305, 1791, 2132, 2140
HspAI	GCGC	2 cut(s)	558, 1080
Kpn2I	TCCGGA	1 cut(s)	951
Ksp22I	TGATCA	1 cut(s)	861
LguI	GCTCTTC	1 cut(s)	525
LmnI	GCTCC	3 cut(s)	661, 1753, 2256
Lsp1109I	GCAGC	7 cut(s)	32, 87, 1277, 1669, 1997, 2073, 2220
LweI	GCATC	3 cut(s)	544, 1697, 2050
MabI	ACCWGGT	1 cut(s)	886
MaeI	CTAG	4 cut(s)	836, 1676, 1877, 2019
MaeII	ACGT	4 cut(s)	305, 1791, 2132, 2140
MaeIII	GTNAC	6 cut(s)	512, 608, 616, 755, 1850, 2068
MbiI	CCGCTC	2 cut(s)	57, 2262
MfeI	CAATTG	1 cut(s)	1083
MflI	RGATCY	5 cut(s)	277, 948, 957, 1507, 1930
MhlI	GDGCHC	5 cut(s)	115, 165, 848, 1594, 2121
MlsI	TGGCCA	2 cut(s)	413, 895
MluNI	TGGCCA	2 cut(s)	413, 895
MlyI	GAGTC	4 cut(s)	77, 224, 782, 2186
MmeI	TCCRAC	1 cut(s)	1505
Mox20I	TGGCCA	2 cut(s)	413, 895
Mph1103I	ATGCAT	1 cut(s)	1801
MroI	TCCGGA	1 cut(s)	951
MscI	TGGCCA	2 cut(s)	413, 895
MseI	TTAA	8 cut(s)	120, 288, 483, 623, 645, 1328, 1388, 2163
MslI	CAYNNNNRTG	1 cut(s)	1611
Msp20I	TGGCCA	2 cut(s)	413, 895
MspI	CCGG	8 cut(s)	328, 394, 541, 733, 875, 952, 1127, 1584
MspR9I	CCNGG	7 cut(s)	135, 329, 733, 888, 1211, 1958, 2083
MunI	CAATTG	1 cut(s)	1083
Mva1269I	GAATGC	1 cut(s)	1000
MvaI	CCWGG	5 cut(s)	135, 888, 1211, 1958, 2083
MvnI	CGCG	1 cut(s)	144
MwoI	GCNNNNNNNGC	9 cut(s)	51, 54, 1088, 1112, 1685, 1737, 1872, 2015, 2268
NciI	CCSGG	2 cut(s)	329, 733
NcoI	CCATGG	2 cut(s)	1492, 2100
NdeI	CATATG	1 cut(s)	634
NlaIV	GGNNCC	6 cut(s)	950, 1509, 1732, 1749, 2024, 2258
NmuCI	GTSAC	6 cut(s)	512, 608, 616, 755, 1850, 2068
NsiI	ATGCAT	1 cut(s)	1801
NspI	RCATGY	2 cut(s)	1716, 2009
PaeR7I	CTCGAG	1 cut(s)	1817
PaqCI	CACCTGC	1 cut(s)	262
PceI	AGGCCT	1 cut(s)	1565
PciSI	GCTCTTC	1 cut(s)	525
PctI	GAATGC	1 cut(s)	1000
PfeI	GAWTC	3 cut(s)	1043, 1486, 2108
PfoI	TCCNGGA	1 cut(s)	327
PleI	GAGTC	4 cut(s)	77, 224, 781, 2186
PpsI	GAGTC	4 cut(s)	77, 224, 781, 2186
Psp124BI	GAGCTC	1 cut(s)	848
Psp6I	CCWGG	5 cut(s)	133, 886, 1209, 1956, 2081
PspGI	CCWGG	5 cut(s)	133, 886, 1209, 1956, 2081
PspN4I	GGNNCC	6 cut(s)	950, 1509, 1732, 1749, 2024, 2258
PspPI	GGNCC	5 cut(s)	735, 1887, 1960, 2022, 2079
PspXI	VCTCGAGB	1 cut(s)	1817
PstI	CTGCAG	3 cut(s)	299, 769, 2154
PsuI	RGATCY	5 cut(s)	277, 948, 957, 1507, 1930
RsaI	GTAC	4 cut(s)	419, 594, 1181, 1812
RsaNI	GTAC	4 cut(s)	418, 593, 1180, 1811
RseI	CAYNNNNRTG	1 cut(s)	1611
SacI	GAGCTC	1 cut(s)	848
SapI	GCTCTTC	1 cut(s)	525
SaqAI	TTAA	8 cut(s)	120, 288, 483, 623, 645, 1328, 1388, 2163
Sau96I	GGNCC	5 cut(s)	735, 1887, 1960, 2022, 2079
ScaI	AGTACT	3 cut(s)	419, 594, 1181
SchI	GAGTC	4 cut(s)	77, 224, 782, 2186
ScrFI	CCNGG	7 cut(s)	135, 329, 733, 888, 1211, 1958, 2083
SduI	GDGCHC	5 cut(s)	115, 165, 848, 1594, 2121
SexAI	ACCWGGT	1 cut(s)	886
SfaNI	GCATC	3 cut(s)	544, 1697, 2050
SfcI	CTRYAG	4 cut(s)	295, 765, 807, 2150
Sfr274I	CTCGAG	1 cut(s)	1817
SinI	GGWCC	1 cut(s)	2079
SlaI	CTCGAG	1 cut(s)	1817
SmiMI	CAYNNNNRTG	1 cut(s)	1611
SmlI	CTYRAG	2 cut(s)	281, 1817
SmoI	CTYRAG	2 cut(s)	281, 1817
SseBI	AGGCCT	1 cut(s)	1565
SsiI	CCGC	9 cut(s)	21, 38, 48, 55, 1091, 1266, 1655, 2251, 2260
SspI	AATATT	1 cut(s)	1630
SspMI	CTAG	4 cut(s)	836, 1676, 1877, 2019
SstI	GAGCTC	1 cut(s)	848
StuI	AGGCCT	1 cut(s)	1565
StyD4I	CCNGG	7 cut(s)	133, 327, 731, 886, 1209, 1956, 2081
StyI	CCWWGG	4 cut(s)	520, 1492, 1566, 2100
TaaI	ACNGT	9 cut(s)	409, 422, 435, 512, 548, 614, 788, 1907, 2224
TaiI	ACGT	4 cut(s)	308, 1794, 2135, 2143
TaqI	TCGA	9 cut(s)	86, 187, 401, 904, 1020, 1818, 2121, 2267, 2274
TaqII	GACCGA	1 cut(s)	939
TatI	WGTACW	4 cut(s)	417, 592, 1179, 1810
TauI	GCSGC	3 cut(s)	57, 1657, 2262
TfiI	GAWTC	3 cut(s)	1043, 1486, 2108
Tru1I	TTAA	8 cut(s)	120, 288, 483, 623, 645, 1328, 1388, 2163
Tru9I	TTAA	8 cut(s)	120, 288, 483, 623, 645, 1328, 1388, 2163
TseFI	GTSAC	6 cut(s)	512, 608, 616, 755, 1850, 2068
TseI	GCWGC	7 cut(s)	45, 100, 1290, 1657, 1985, 2086, 2208
Tsp45I	GTSAC	6 cut(s)	512, 608, 616, 755, 1850, 2068
TspDTI	ATGAA	8 cut(s)	189, 330, 615, 1038, 1188, 1243, 1750, 2084
TspGWI	ACGGA	1 cut(s)	1923
VneI	GTGCAC	1 cut(s)	1590
VpaK11BI	GGWCC	1 cut(s)	2079
XapI	RAATTY	2 cut(s)	318, 337
XceI	RCATGY	2 cut(s)	1716, 2009
XcmI	CCANNNNNNNNNTGG	1 cut(s)	942
XhoI	CTCGAG	1 cut(s)	1817
XmiI	GTMKAC	1 cut(s)	994
XspI	CTAG	4 cut(s)	836, 1676, 1877, 2019
ZrmI	AGTACT	3 cut(s)	419, 594, 1181
Zsp2I	ATGCAT	1 cut(s)	1801

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.