Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0011788

Physical Location & Seq

Genomic Coordinates Forward (+)

13326363 .. 13330874

Gene Structure 4512 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1171600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1308 bp

ATGAACAAGTCGAGAAAGGTAGGGAAGTATGAGGTGGGGCGGATGGTTGGCGAAGGGACATTCGGCAAGGTTAAGTTTGCGAGGAACTGCGAGACCGGCGAAAGCGTGGCCATGAAGATTTTGGCCAAAAGCACCATTCTCAAGCACAGAATGGTTGATCAGATTAAAAGAGAGATATCAATAATGAAGATTGTCAGGCATCCTAATATAGTAAGGCTGCATGAGGTTTTGGCTGGTCGAGCGAAGATATTTATAATTCTCGAGTTTGTAACTGGAGGAGAATTGTTTGATAAAATTGTTCACCGAGGAAAGCTTCCTGAAAATGAATCCAGGAAATACTTTCAACAGCTTGTGGATGCAGTTGCCCACTGTCACAGTAAGGGTGTGTACCACAGAGACCTGAAGCCCGAAAATCTTCTCCTTGATGCTTATGGAAATTTGAAGGTTTCTGATTTTGGACTAAGTGCATTGCCCCTGCAAGGGGACGGTCTTCTTCGCACCACATGTGGAACCCCAAACTATGTTGCTCCTGAGGTGCTCGGCGATCAGGGTTACGATGGAGCTGCTGCTGATATTTGGTCATGTGGGGTCATCCTGTATGTTCTGATGGCTGGATACCTCCCATTTGATGAGACAAATCTATATACCCTCTATAGCAAAATTACTGCAGCAGAGTTCTCCTGTCCATATTGGTTTTCTCCCGAGGCAAATAAATTGATACAAAAGATACTTGATCCCAATCCTAAAACTCGAATTCGGATTGATGGTATCAGAAATGATCCATGGTTTAAGAAAAATTATGCTCCTGTCAAATATAGAGAAGATGAGGAAGTAAGCTTGGATGATGTTCGTGCAGTCTTTGAGGATATTGAGGATCAATATATAGCGGAGCGGTCAGAAATTAAGGATGGTGGTCCTTTGTTAATGAATGCGTTCGAGATGATTACCCTATCCCAAGGGCTAAATCTATCTGCATTGTTTGACCGAAGACAGGATTATGTAAATCGGCAAACCCGGTTTGTTTCCCGCAAACCAGCTCAAGTTATAATTTCAAATGTAGAAGCTGTTGCAGAATCAATGAGTCTCAAGGTCCATACGCGCAATTACAAGACAAGACTTGAAGGAATATCAGCAAATAGGACGGGACAATTTGCAGTTGTCCTCGAGGTTTATGAAGTTGCACCATCCCTTTTCATGGTAGATGTTCGGAAGGCCGCAGGAGATACTCTTGAATATCATAAGTTTTACAAGAATTTCTGTGCCAAATTAGAAGATATTATTTGGAACCCAATAGAGGGTACGGGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009628 GO:0009651 GO:0009705 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0031090 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0098588 GO:0098805 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04068 ko04140 ko04150 ko04151 ko04152 ko04211 ko04213 ko04371 ko04530 ko04710 ko04714 ko04910 ko04920 ko04921 ko04922 ko04931 ko04932 ko05410 ko05418 map04068 map04140 map04150 map04151 map04152 map04211 map04213 map04371 map04530 map04710 map04714 map04910 map04920 map04921 map04922 map04931 map04932 map05410 map05418

Pfam Domains

Protein Families

NAF Pkinase

Protein Analysis

436

Amino Acids

49.31

Weight (kDa)

8.32

Isoelectric Point (pI)

30.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	10 - 263	5.9e-72	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	12 - 258	1.3e-35	Protein tyrosine and serine/threonine kinase
Kinase-like	PF14531	110 - 212	3.8e-07	Kinase-like
NAF	PF03822	306 - 365	3.1e-19	NAF domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011788)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G35410 AT5G35410
fragaria_vesca	FvH4_1g03090 FvH4_1g03090 FvH4_1g03090
malus_domestica	MD02G1030100.v1.1 MD15G1171600.v1.1
prunus_persica	Prupe.7G244500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0088231
rosa_laevigata	RLG00000015934
rosa_multiflora	Rmu_sc0004235.1_g000049
rosa_roxburghii	Rroxscaffold_2G00152820
rosa_rugosa	Rorug01G0482000 Rorug01G0482000 Rorug01G0482100 Rorug01G0482200
rosa_samantha	Rh2AG037200 Rh2BG036400
rosa_wichuraiana	Rw2G002920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	254, 1046
AbsI	CCTCGAGG	1 cut(s)	1163
AccBSI	CCGCTC	1 cut(s)	892
AccII	CGCG	1 cut(s)	1099
AciI	CCGC	5 cut(s)	40, 887, 892, 1027, 1215
AclWI	GGATC	3 cut(s)	728, 773, 882
AcoI	YGGCCR	2 cut(s)	108, 123
AcsI	RAATTY	3 cut(s)	436, 753, 1252
AcuI	CTGAAG	1 cut(s)	422
AfaI	GTAC	2 cut(s)	389, 1300
AfiI	CCNNNNNNNGG	7 cut(s)	479, 480, 481, 991, 1195, 1294, 1295
AflIII	ACRYGT	1 cut(s)	503
AgsI	TTSAA	5 cut(s)	344, 442, 1053, 1121, 1232
AjnI	CCWGG	1 cut(s)	329
AluBI	AGCT	6 cut(s)	313, 349, 563, 837, 1037, 1064
AluI	AGCT	6 cut(s)	313, 349, 563, 837, 1037, 1064
Alw21I	GWGCWC	1 cut(s)	540
Alw26I	GTCTC	4 cut(s)	86, 390, 626, 1088
AlwI	GGATC	3 cut(s)	728, 773, 882
Ama87I	CYCGRG	3 cut(s)	260, 701, 1163
AoxI	GGCC	3 cut(s)	108, 123, 1212
ApeKI	GCWGC	4 cut(s)	217, 563, 566, 668
ApoI	RAATTY	3 cut(s)	436, 753, 1252
Asp700I	GAANNNNTTC	2 cut(s)	414, 932
AspLEI	GCGC	1 cut(s)	1101
AspS9I	GGNCC	2 cut(s)	914, 1090
AsuC2I	CCSGG	1 cut(s)	1015
AsuHPI	GGTGA	1 cut(s)	293
AvaI	CYCGRG	3 cut(s)	260, 701, 1163
AvaII	GGWCC	2 cut(s)	914, 1090
AxyI	CCTNAGG	1 cut(s)	531
BalI	TGGCCA	2 cut(s)	110, 125
BbsI	GAAGAC	2 cut(s)	482, 994
Bbv12I	GWGCWC	1 cut(s)	540
BbvI	GCAGC	4 cut(s)	204, 550, 553, 680
BccI	CCATC	6 cut(s)	37, 551, 601, 758, 902, 1192
BcgI	CGANNNNNNTGC	2 cut(s)	545, 579
BciT130I	CCWGG	1 cut(s)	331
BciVI	GTATCC	1 cut(s)	608
BclI	TGATCA	1 cut(s)	157
BcnI	CCSGG	1 cut(s)	1015
BcoDI	GTCTC	4 cut(s)	86, 390, 626, 1088
BfmI	CTRYAG	2 cut(s)	652, 666
BfuI	GTATCC	1 cut(s)	608
BisI	GCNGC	5 cut(s)	218, 564, 567, 669, 1215
BlsI	GCNGC	5 cut(s)	219, 565, 568, 670, 1216
Bme1390I	CCNGG	2 cut(s)	331, 1015
Bme18I	GGWCC	2 cut(s)	914, 1090
BmeT110I	CYCGRG	3 cut(s)	260, 701, 1163
BmgT120I	GGNCC	2 cut(s)	914, 1090
BmiI	GGNNCC	2 cut(s)	511, 1286
BmrFI	CCNGG	2 cut(s)	331, 1015
BmsI	GCATC	3 cut(s)	208, 346, 415
BpiI	GAAGAC	2 cut(s)	482, 994
BpmI	CTGGAG	1 cut(s)	294
BpuEI	CTTGAG	3 cut(s)	125, 1023, 1070
BpuMI	CCSGG	1 cut(s)	1015
BsaBI	GATNNNNATC	1 cut(s)	738
BsaI	GGTCTC	2 cut(s)	86, 390
BsaJI	CCNNGG	4 cut(s)	304, 702, 782, 955
Bsc4I	CCNNNNNNNGG	7 cut(s)	479, 480, 481, 991, 1195, 1294, 1295
Bse118I	RCCGGY	1 cut(s)	95
Bse1I	ACTGG	1 cut(s)	277
Bse21I	CCTNAGG	1 cut(s)	531
Bse3DI	GCAATG	1 cut(s)	467
Bse8I	GATNNNNATC	1 cut(s)	738
BseBI	CCWGG	1 cut(s)	331
BseDI	CCNNGG	4 cut(s)	304, 702, 782, 955
BseGI	GGATG	7 cut(s)	48, 199, 361, 591, 847, 913, 1184
BseJI	GATNNNNATC	1 cut(s)	738
BseLI	CCNNNNNNNGG	7 cut(s)	479, 480, 481, 991, 1195, 1294, 1295
BseMI	GCAATG	1 cut(s)	467
BseMII	CTCAG	1 cut(s)	522
BseNI	ACTGG	1 cut(s)	277
BseRI	GAGGAG	1 cut(s)	291
BseXI	GCAGC	4 cut(s)	204, 550, 553, 680
BsgI	GTGCAG	1 cut(s)	873
Bsh1236I	CGCG	1 cut(s)	1099
BshFI	GGCC	3 cut(s)	110, 125, 1214
BsiHKAI	GWGCWC	1 cut(s)	540
BsiHKCI	CYCGRG	3 cut(s)	260, 701, 1163
BsiSI	CCGG	2 cut(s)	96, 1015
BslFI	GGGAC	3 cut(s)	70, 497, 1158
BslI	CCNNNNNNNGG	7 cut(s)	479, 480, 481, 991, 1195, 1294, 1295
BsmAI	GTCTC	4 cut(s)	86, 390, 626, 1088
BsmFI	GGGAC	3 cut(s)	70, 497, 1158
BsmI	GAATGC	1 cut(s)	934
BsnI	GGCC	3 cut(s)	110, 125, 1214
Bso31I	GGTCTC	2 cut(s)	86, 390
BsoBI	CYCGRG	3 cut(s)	260, 701, 1163
Bsp1286I	GDGCHC	1 cut(s)	540
Bsp143I	GATC	5 cut(s)	157, 544, 733, 778, 874
Bsp19I	CCATGG	1 cut(s)	782
BspACI	CCGC	5 cut(s)	40, 887, 892, 1027, 1215
BspANI	GGCC	3 cut(s)	110, 125, 1214
BspCNI	CTCAG	1 cut(s)	523
BspFNI	CGCG	1 cut(s)	1099
BspLI	GGNNCC	2 cut(s)	511, 1286
BspMAI	CTGCAG	1 cut(s)	670
BspPI	GGATC	3 cut(s)	728, 773, 882
BspTNI	GGTCTC	2 cut(s)	86, 390
BsrBI	CCGCTC	1 cut(s)	892
BsrDI	GCAATG	1 cut(s)	467
BsrFI	RCCGGY	1 cut(s)	95
BsrI	ACTGG	1 cut(s)	277
BssAI	RCCGGY	1 cut(s)	95
BssECI	CCNNGG	4 cut(s)	304, 702, 782, 955
BssMI	GATC	5 cut(s)	157, 544, 733, 778, 874
BssT1I	CCWWGG	2 cut(s)	782, 955
Bst2UI	CCWGG	1 cut(s)	331
Bst4CI	ACNGT	3 cut(s)	371, 377, 488
BstDEI	CTNAG	2 cut(s)	461, 531
BstDSI	CCRYGG	1 cut(s)	782
BstF5I	GGATG	7 cut(s)	48, 199, 361, 591, 847, 913, 1184
BstFNI	CGCG	1 cut(s)	1099
BstHHI	GCGC	1 cut(s)	1101
BstKTI	GATC	5 cut(s)	160, 547, 736, 781, 877
BstMAI	GTCTC	4 cut(s)	86, 390, 626, 1088
BstMBI	GATC	5 cut(s)	157, 544, 733, 778, 874
BstMWI	GCNNNNNNNGC	2 cut(s)	96, 239
BstNI	CCWGG	1 cut(s)	331
BstNSI	RCATGY	1 cut(s)	507
BstSCI	CCNGG	2 cut(s)	329, 1013
BstSFI	CTRYAG	2 cut(s)	652, 666
BstUI	CGCG	1 cut(s)	1099
BstV1I	GCAGC	4 cut(s)	204, 550, 553, 680
BstV2I	GAAGAC	2 cut(s)	482, 994
Bsu36I	CCTNAGG	1 cut(s)	531
BsuI	GTATCC	1 cut(s)	608
BsuRI	GGCC	3 cut(s)	110, 125, 1214
BtgI	CCRYGG	1 cut(s)	782
BtsCI	GGATG	7 cut(s)	48, 199, 361, 591, 847, 913, 1184
BtsIMutI	CAGTG	1 cut(s)	367
CfoI	GCGC	1 cut(s)	1101
Cfr10I	RCCGGY	1 cut(s)	95
Cfr13I	GGNCC	2 cut(s)	914, 1090
Csp6I	GTAC	2 cut(s)	388, 1299
CviAII	CATG	6 cut(s)	112, 221, 504, 582, 783, 1195
CviQI	GTAC	2 cut(s)	388, 1299
DdeI	CTNAG	2 cut(s)	461, 531
DpnI	GATC	5 cut(s)	159, 546, 735, 780, 876
DpnII	GATC	5 cut(s)	157, 544, 733, 778, 874
EaeI	YGGCCR	2 cut(s)	108, 123
EciI	GGCGGA	1 cut(s)	55
Eco130I	CCWWGG	2 cut(s)	782, 955
Eco31I	GGTCTC	2 cut(s)	86, 390
Eco32I	GATATC	1 cut(s)	177
Eco47I	GGWCC	2 cut(s)	914, 1090
Eco57I	CTGAAG	1 cut(s)	422
Eco81I	CCTNAGG	1 cut(s)	531
Eco88I	CYCGRG	3 cut(s)	260, 701, 1163
EcoRI	GAATTC	1 cut(s)	753
EcoRII	CCWGG	1 cut(s)	329
EcoRV	GATATC	1 cut(s)	177
EcoT14I	CCWWGG	2 cut(s)	782, 955
ErhI	CCWWGG	2 cut(s)	782, 955
FaeI	CATG	6 cut(s)	115, 224, 507, 585, 786, 1198
FaqI	GGGAC	3 cut(s)	70, 497, 1158
FatI	CATG	6 cut(s)	111, 220, 503, 581, 782, 1194
FauI	CCCGC	1 cut(s)	1034
FbaI	TGATCA	1 cut(s)	157
Fnu4HI	GCNGC	5 cut(s)	218, 564, 567, 669, 1215
FokI	GGATG	7 cut(s)	55, 186, 368, 578, 854, 920, 1171
Fsp4HI	GCNGC	5 cut(s)	218, 564, 567, 669, 1215
GlaI	GCGC	1 cut(s)	1100
GluI	GCNGC	5 cut(s)	218, 564, 567, 669, 1215
GsuI	CTGGAG	1 cut(s)	294
HaeIII	GGCC	3 cut(s)	110, 125, 1214
HapII	CCGG	2 cut(s)	96, 1015
HhaI	GCGC	1 cut(s)	1101
Hin1II	CATG	6 cut(s)	115, 224, 507, 585, 786, 1198
Hin6I	GCGC	1 cut(s)	1099
HinP1I	GCGC	1 cut(s)	1099
HindIII	AAGCTT	2 cut(s)	311, 835
HinfI	GANTC	3 cut(s)	326, 1073, 1081
HpaII	CCGG	2 cut(s)	96, 1015
HphI	GGTGA	1 cut(s)	293
Hpy166II	GTNNAC	2 cut(s)	301, 388
Hpy188I	TCNGA	7 cut(s)	162, 451, 606, 759, 773, 898, 1209
Hpy188III	TCNNGA	7 cut(s)	12, 260, 317, 530, 701, 937, 1229
Hpy8I	GTNNAC	2 cut(s)	301, 388
HpyAV	CCTTC	4 cut(s)	47, 436, 1115, 1204
HpyCH4III	ACNGT	3 cut(s)	371, 377, 488
HpyF10VI	GCNNNNNNNGC	2 cut(s)	96, 239
HpyF3I	CTNAG	2 cut(s)	461, 531
Hsp92II	CATG	6 cut(s)	115, 224, 507, 585, 786, 1198
HspAI	GCGC	1 cut(s)	1099
Ksp22I	TGATCA	1 cut(s)	157
Kzo9I	GATC	5 cut(s)	157, 544, 733, 778, 874
LmnI	GCTCC	4 cut(s)	532, 560, 808, 889
Lsp1109I	GCAGC	4 cut(s)	204, 550, 553, 680
LweI	GCATC	3 cut(s)	208, 346, 415
MaeIII	GTNAC	3 cut(s)	268, 371, 551
MalI	GATC	5 cut(s)	159, 546, 735, 780, 876
MbiI	CCGCTC	1 cut(s)	892
MboI	GATC	5 cut(s)	157, 544, 733, 778, 874
MboII	GAAGA	9 cut(s)	127, 199, 256, 407, 482, 485, 833, 999, 1283
MhlI	GDGCHC	1 cut(s)	540
MlsI	TGGCCA	2 cut(s)	110, 125
MluNI	TGGCCA	2 cut(s)	110, 125
MlyI	GAGTC	1 cut(s)	1090
Mox20I	TGGCCA	2 cut(s)	110, 125
MroXI	GAANNNNTTC	2 cut(s)	414, 932
MscI	TGGCCA	2 cut(s)	110, 125
MseI	TTAA	5 cut(s)	72, 165, 789, 903, 923
Msp20I	TGGCCA	2 cut(s)	110, 125
MspI	CCGG	2 cut(s)	96, 1015
MspR9I	CCNGG	2 cut(s)	331, 1015
Mva1269I	GAATGC	1 cut(s)	934
MvaI	CCWGG	1 cut(s)	331
MvnI	CGCG	1 cut(s)	1099
MwoI	GCNNNNNNNGC	2 cut(s)	96, 239
NciI	CCSGG	1 cut(s)	1015
NcoI	CCATGG	1 cut(s)	782
NdeII	GATC	5 cut(s)	157, 544, 733, 778, 874
NlaIII	CATG	6 cut(s)	115, 224, 507, 585, 786, 1198
NlaIV	GGNNCC	2 cut(s)	511, 1286
NmeAIII	GCCGAG	1 cut(s)	519
NmuCI	GTSAC	1 cut(s)	371
NspI	RCATGY	1 cut(s)	507
PaeR7I	CTCGAG	2 cut(s)	260, 1163
PciI	ACATGT	1 cut(s)	503
PctI	GAATGC	1 cut(s)	934
PdmI	GAANNNNTTC	2 cut(s)	414, 932
PfeI	GAWTC	2 cut(s)	326, 1073
PfoI	TCCNGGA	1 cut(s)	329
PkrI	GCNGC	5 cut(s)	219, 565, 568, 670, 1216
PleI	GAGTC	1 cut(s)	1089
PpsI	GAGTC	1 cut(s)	1089
PscI	ACATGT	1 cut(s)	503
PsiI	TTATAA	2 cut(s)	254, 1046
Psp6I	CCWGG	1 cut(s)	329
PspGI	CCWGG	1 cut(s)	329
PspN4I	GGNNCC	2 cut(s)	511, 1286
PspPI	GGNCC	2 cut(s)	914, 1090
PspXI	VCTCGAGB	1 cut(s)	1163
PstI	CTGCAG	1 cut(s)	670
RsaI	GTAC	2 cut(s)	389, 1300
RsaNI	GTAC	2 cut(s)	388, 1299
SaqAI	TTAA	5 cut(s)	72, 165, 789, 903, 923
SatI	GCNGC	5 cut(s)	218, 564, 567, 669, 1215
Sau3AI	GATC	5 cut(s)	157, 544, 733, 778, 874
Sau96I	GGNCC	2 cut(s)	914, 1090
SchI	GAGTC	1 cut(s)	1090
ScrFI	CCNGG	2 cut(s)	331, 1015
SduI	GDGCHC	1 cut(s)	540
SfaNI	GCATC	3 cut(s)	208, 346, 415
SfcI	CTRYAG	2 cut(s)	652, 666
Sfr274I	CTCGAG	2 cut(s)	260, 1163
SinI	GGWCC	2 cut(s)	914, 1090
SlaI	CTCGAG	2 cut(s)	260, 1163
SmlI	CTYRAG	5 cut(s)	140, 260, 1038, 1085, 1163
SmoI	CTYRAG	5 cut(s)	140, 260, 1038, 1085, 1163
SsiI	CCGC	5 cut(s)	40, 887, 892, 1027, 1215
StyD4I	CCNGG	2 cut(s)	329, 1013
StyI	CCWWGG	2 cut(s)	782, 955
TaaI	ACNGT	3 cut(s)	371, 377, 488
TaqI	TCGA	6 cut(s)	11, 238, 261, 751, 936, 1164
TaqII	GACCGA	1 cut(s)	999
TauI	GCSGC	1 cut(s)	1217
TfiI	GAWTC	2 cut(s)	326, 1073
Tru1I	TTAA	5 cut(s)	72, 165, 789, 903, 923
Tru9I	TTAA	5 cut(s)	72, 165, 789, 903, 923
TscAI	CASTG	1 cut(s)	374
TseFI	GTSAC	1 cut(s)	371
TseI	GCWGC	4 cut(s)	217, 563, 566, 668
Tsp45I	GTSAC	1 cut(s)	371
TspDTI	ATGAA	7 cut(s)	17, 128, 200, 339, 941, 1183, 1188
TspRI	CASTG	1 cut(s)	374
VpaK11BI	GGWCC	2 cut(s)	914, 1090
XapI	RAATTY	3 cut(s)	436, 753, 1252
XceI	RCATGY	1 cut(s)	507
XcmI	CCANNNNNNNNNTGG	1 cut(s)	118
XhoI	CTCGAG	2 cut(s)	260, 1163
XmnI	GAANNNNTTC	2 cut(s)	414, 932

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.