MD15G1174500.v1.1

NHP2-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013069
Physical Location & Seq
Reverse (-)
13595181 .. 13597059
1879 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1174500.v1.1.491

Sequence Viewer

Length: 387 bp
ATGACCGGAGAGGCAGTGAACCCGAAAGCGTACCCGTTGGCGGACGCGCAACTGACGATCACCATACTCGACCTGGTTCAGCAAGCTGCTAACTACAAGCAGCTCAAAAAGGGCGCGAATGAAGCAACTAAAACCTTGAACAGGGGTATTTCGGAGTTTATAGTGATGGCGGCTGATACTGAGCCTCTGGAGATTCTTCTTCACCTTCCCCTGTTGGCTGAGGATAAGAATGTGCCGTATGTGTTTGTCCCTTCGAAGCAAGCACTTGGACGAGCATGCGGTGTCACAAGACCCGTCATTGCCGCCTCTGTGACAACAAACGAGGGAAGTCAATTGAAGTCACAAATACAACAACTCAAGGATGCCATTGAGAAGCTCCTTATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 ko03040 map03008 map03040
Pfam Domains
Protein Families
Ribosomal_L7Ae

Protein Analysis

129

Amino Acids

13.84

Weight (kDa)

6.57

Isoelectric Point (pI)

34.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L7Ae PF01248 23 - 111 5.6e-27 Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013069)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G12600 AT4G22380 AT5G20160
fragaria_vesca FvH4_1g03460
malus_domestica MD02G1033700.v1.1 MD15G1174500.v1.1
prunus_persica Prupe.5G003200_v2.0.a1 Prupe.7G241400_v2.0.a1
pyrus_communis pycom02g02760 pycom15g15610
rosa_chinensis RchiOBHm_Chr2g0088681
rosa_laevigata RLG00000015975
rosa_multiflora Rmu_sc0006835.1_g000011 Rmu_sc0015442.1_g000014
rosa_roxburghii Rroxscaffold_2G00152410
rosa_rugosa Rorug01G0485800
rosa_samantha Rh2AG041300
rosa_wichuraiana Rw2G003310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 47, 116
AciI CCGC 4 cut(s) 41, 170, 279, 303
AfaI GTAC 1 cut(s) 32
AfiI CCNNNNNNNGG 2 cut(s) 40, 141
AgsI TTSAA 2 cut(s) 139, 337
AjnI CCWGG 1 cut(s) 72
AluBI AGCT 3 cut(s) 86, 103, 376
AluI AGCT 3 cut(s) 86, 103, 376
ApeKI GCWGC 2 cut(s) 86, 100
AspLEI GCGC 2 cut(s) 49, 116
AsuHPI GGTGA 2 cut(s) 52, 194
AsuII TTCGAA 1 cut(s) 254
BbvCI CCTCAGC 1 cut(s) 219
BbvI GCAGC 2 cut(s) 73, 112
BccI CCATC 1 cut(s) 160
BceAI ACGGC 1 cut(s) 220
BciT130I CCWGG 1 cut(s) 74
BisI GCNGC 4 cut(s) 87, 101, 171, 303
BlsI GCNGC 4 cut(s) 88, 102, 172, 304
Bme1390I CCNGG 1 cut(s) 74
BmrFI CCNGG 1 cut(s) 74
BmsI GCATC 1 cut(s) 352
BpmI CTGGAG 1 cut(s) 209
Bpu10I CCTNAGC 1 cut(s) 219
Bpu14I TTCGAA 1 cut(s) 254
BpuEI CTTGAG 1 cut(s) 341
BsaWI WCCGGW 1 cut(s) 5
BsaXI ACNNNNNCTCC 1 cut(s) 30
Bsc4I CCNNNNNNNGG 2 cut(s) 40, 141
Bse3DI GCAATG 1 cut(s) 297
BseBI CCWGG 1 cut(s) 74
BseGI GGATG 1 cut(s) 367
BseLI CCNNNNNNNGG 2 cut(s) 40, 141
BseMI GCAATG 1 cut(s) 297
BseMII CTCAG 2 cut(s) 171, 210
BseXI GCAGC 2 cut(s) 73, 112
Bsh1236I CGCG 2 cut(s) 47, 116
BsiSI CCGG 1 cut(s) 6
BslFI GGGAC 1 cut(s) 233
BslI CCNNNNNNNGG 2 cut(s) 40, 141
BsmFI GGGAC 1 cut(s) 233
Bsp119I TTCGAA 1 cut(s) 254
Bsp143I GATC 1 cut(s) 57
BspACI CCGC 4 cut(s) 41, 170, 279, 303
BspCNI CTCAG 2 cut(s) 172, 211
BspFNI CGCG 2 cut(s) 47, 116
BspT104I TTCGAA 1 cut(s) 254
BsrDI GCAATG 1 cut(s) 297
BssMI GATC 1 cut(s) 57
Bst2UI CCWGG 1 cut(s) 74
BstBI TTCGAA 1 cut(s) 254
BstC8I GCNNGC 3 cut(s) 84, 261, 277
BstDEI CTNAG 2 cut(s) 180, 219
BstENI CCTNNNNNAGG 1 cut(s) 139
BstF5I GGATG 1 cut(s) 367
BstFNI CGCG 2 cut(s) 47, 116
BstHHI GCGC 2 cut(s) 49, 116
BstKTI GATC 1 cut(s) 60
BstMBI GATC 1 cut(s) 57
BstMWI GCNNNNNNNGC 1 cut(s) 122
BstNI CCWGG 1 cut(s) 74
BstNSI RCATGY 1 cut(s) 279
BstSCI CCNGG 1 cut(s) 72
BstUI CGCG 2 cut(s) 47, 116
BstV1I GCAGC 2 cut(s) 73, 112
BtsCI GGATG 1 cut(s) 367
BtsI GCAGTG 1 cut(s) 21
BtsIMutI CAGTG 1 cut(s) 21
Cac8I GCNNGC 3 cut(s) 84, 261, 277
CfoI GCGC 2 cut(s) 49, 116
CseI GACGC 1 cut(s) 53
CsiI ACCWGGT 1 cut(s) 72
Csp6I GTAC 1 cut(s) 31
CviAII CATG 1 cut(s) 276
CviJI RGCY 6 cut(s) 86, 103, 173, 184, 218, 376
CviKI_1 RGCY 6 cut(s) 86, 103, 173, 184, 218, 376
CviQI GTAC 1 cut(s) 31
DdeI CTNAG 2 cut(s) 180, 219
DpnI GATC 1 cut(s) 59
DpnII GATC 1 cut(s) 57
EciI GGCGGA 1 cut(s) 56
EcoNI CCTNNNNNAGG 1 cut(s) 139
EcoRII CCWGG 1 cut(s) 72
FaeI CATG 1 cut(s) 279
FaiI YATR 4 cut(s) 65, 161, 240, 277
FaqI GGGAC 1 cut(s) 233
FatI CATG 1 cut(s) 275
Fnu4HI GCNGC 4 cut(s) 87, 101, 171, 303
FokI GGATG 1 cut(s) 374
Fsp4HI GCNGC 4 cut(s) 87, 101, 171, 303
GlaI GCGC 2 cut(s) 48, 115
GluI GCNGC 4 cut(s) 87, 101, 171, 303
GsuI CTGGAG 1 cut(s) 209
HapII CCGG 1 cut(s) 6
HgaI GACGC 1 cut(s) 53
HhaI GCGC 2 cut(s) 49, 116
Hin1II CATG 1 cut(s) 279
Hin6I GCGC 2 cut(s) 47, 114
HinP1I GCGC 2 cut(s) 47, 114
HinfI GANTC 1 cut(s) 193
HpaII CCGG 1 cut(s) 6
HphI GGTGA 2 cut(s) 52, 194
Hpy166II GTNNAC 1 cut(s) 19
Hpy188I TCNGA 1 cut(s) 154
Hpy188III TCNNGA 1 cut(s) 188
Hpy8I GTNNAC 1 cut(s) 19
HpyAV CCTTC 2 cut(s) 215, 261
HpyF10VI GCNNNNNNNGC 1 cut(s) 122
HpyF3I CTNAG 2 cut(s) 180, 219
Hsp92II CATG 1 cut(s) 279
HspAI GCGC 2 cut(s) 47, 114
Kzo9I GATC 1 cut(s) 57
LmnI GCTCC 1 cut(s) 381
LpnPI CCDG 6 cut(s) 19, 59, 86, 127, 173, 224
Lsp1109I GCAGC 2 cut(s) 73, 112
LweI GCATC 1 cut(s) 352
MabI ACCWGGT 1 cut(s) 72
MaeIII GTNAC 3 cut(s) 283, 310, 339
MalI GATC 1 cut(s) 59
MboI GATC 1 cut(s) 57
MboII GAAGA 2 cut(s) 188, 191
MfeI CAATTG 1 cut(s) 332
MluCI AATT 1 cut(s) 332
MnlI CCTC 5 cut(s) 4, 195, 214, 316, 316
MspI CCGG 1 cut(s) 6
MspR9I CCNGG 1 cut(s) 74
MunI CAATTG 1 cut(s) 332
MvaI CCWGG 1 cut(s) 74
MvnI CGCG 2 cut(s) 47, 116
MwoI GCNNNNNNNGC 1 cut(s) 122
NdeII GATC 1 cut(s) 57
NlaIII CATG 1 cut(s) 279
NmuCI GTSAC 3 cut(s) 283, 310, 339
NspI RCATGY 1 cut(s) 279
NspV TTCGAA 1 cut(s) 254
PaeI GCATGC 1 cut(s) 279
PfeI GAWTC 1 cut(s) 193
PkrI GCNGC 4 cut(s) 88, 102, 172, 304
Psp6I CCWGG 1 cut(s) 72
PspGI CCWGG 1 cut(s) 72
RsaI GTAC 1 cut(s) 32
RsaNI GTAC 1 cut(s) 31
SatI GCNGC 4 cut(s) 87, 101, 171, 303
Sau3AI GATC 1 cut(s) 57
ScrFI CCNGG 1 cut(s) 74
SetI ASST 6 cut(s) 75, 88, 105, 137, 207, 378
SexAI ACCWGGT 1 cut(s) 72
SfaNI GCATC 1 cut(s) 352
SfuI TTCGAA 1 cut(s) 254
SmlI CTYRAG 1 cut(s) 356
SmoI CTYRAG 1 cut(s) 356
SphI GCATGC 1 cut(s) 279
Sse9I AATT 1 cut(s) 332
SsiI CCGC 4 cut(s) 41, 170, 279, 303
StyD4I CCNGG 1 cut(s) 72
TaqI TCGA 2 cut(s) 69, 254
TasI AATT 1 cut(s) 332
TauI GCSGC 2 cut(s) 173, 305
TfiI GAWTC 1 cut(s) 193
TscAI CASTG 1 cut(s) 21
TseFI GTSAC 3 cut(s) 283, 310, 339
TseI GCWGC 2 cut(s) 86, 100
Tsp45I GTSAC 3 cut(s) 283, 310, 339
TspDTI ATGAA 1 cut(s) 135
TspRI CASTG 1 cut(s) 21
XagI CCTNNNNNAGG 1 cut(s) 139
XceI RCATGY 1 cut(s) 279
XcmI CCANNNNNNNNNTGG 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.