MD15G1229800.v1.1

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008979
Physical Location & Seq
Forward (+)
18839991 .. 18842710
2720 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1229800.v1.1.491

Sequence Viewer

Length: 1155 bp
ATGGCTTCAGGAACTGCAGATATCTCGCCACAAGTCACCGGATTGGATGGTGGGTGCTTAAACGGAGGTGGAATGCCGATCATGGAAGATCAAAACGTCGACGCTTTGGCCAATTCGAACCACATTCCAACCGGAAAGCCTCCGAGAAACCTCTCGGTTATGCGAAATTGCACTAGCTATGCATTGTTGACCGAATCAGATTCAGATATTGGAAGTATGGGATTGAAGTCACCGGCAAGTGAGACAGCTGAGTTCGTATTTGTATTCCGTTCAGGAAGCTGCTCTGAGAAAGGACCTAAACAGTACATGGAGGATGAGTACATTTGCGTAGATAATCTCCATGATCATATTGCTGAAATTGAAAACTTGCCTTCTCCTGGGGCATTTTATGGGGTCTTTGATGGACATAATGGTGTTGATGCAGCGTCATTCATCAAAAGCAACATCCTTAAATTCATCGTTGAAGACTCTCACTTCCCATCTGGCGTTAAAAAGGCAGTTAGAAGCGCTTTTGTGAAGGCTGATCATGCTTTTGCAGATGCTAGCTCAGTTGACAAGTCCTCCGGTACCACTGCCTTGACTGCCCTGATCTTAGGAAGGACCATGCTAATCGCTAATGCTGGGGACTGTCGAGCTGTGTTGGGCAAGCGGGGAAGGGCAATTGAGCTATCTAAAGATCACAAGCCTAACTGCACCTCTGAAAGATTAAGAATTGAAAAGCTGGGTGGTGTTATCTATGATGGCTACCTCAATGGCCAACTATCCGTAGCGCGTGCTCTTGGAGACTGGCATATCAAGGGCTCGAAAGGTTCAAAAAGCCCCTTGAGCTCCGAGCCAGAGTTGGAGGAAATTGTCCTAACAGAAGAAGACGAGTTCTTGATAATGGGCTGTGATGGCTTGTGGGATGTGATGAGCAGCCAGTGCGCAGTTACAATGGTGAGGAAGGAGCTCATGCAGCACAATGATCCCGAGAGATGTTCAAAAGCGCTCGTCAAGGAAGCGCTCCAGCGAAACACGTGCGATAACCTCACTGTTGTCGTGGTCTGTTTCTCCGAAGATCCACCTCCTAAAACCGAAATATCTAAATCCCACAAGAGAAGGAGCATTTCAGCTGAAGGGTTGGACCTTCTCAAGGGTGTCTTAAGCACCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

385

Amino Acids

41.33

Weight (kDa)

5.37

Isoelectric Point (pI)

56.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 91 - 342 5e-71 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 925
Acc65I GGTACC 1 cut(s) 566
AccB1I GGYRCC 1 cut(s) 566
AccI GTMKAC 1 cut(s) 99
AccII CGCG 1 cut(s) 772
AciI CCGC 1 cut(s) 649
AclWI GGATC 2 cut(s) 959, 1052
AcoI YGGCCR 2 cut(s) 108, 754
AcsI RAATTY 1 cut(s) 452
AcuI CTGAAG 1 cut(s) 1134
AcvI CACGTG 1 cut(s) 1017
AfaI GTAC 3 cut(s) 305, 320, 568
AfeI AGCGCT 3 cut(s) 508, 987, 1002
AfiI CCNNNNNNNGG 2 cut(s) 377, 1132
AflII CTTAAG 1 cut(s) 1141
AflIII ACRYGT 1 cut(s) 1014
AgsI TTSAA 6 cut(s) 226, 362, 464, 716, 813, 981
AjnI CCWGG 1 cut(s) 376
Alw21I GWGCWC 3 cut(s) 778, 830, 951
Alw26I GTCTC 2 cut(s) 236, 777
AlwI GGATC 2 cut(s) 959, 1052
AlwNI CAGNNNCTG 1 cut(s) 14
Ama87I CYCGRG 1 cut(s) 968
Aor51HI AGCGCT 3 cut(s) 508, 987, 1002
AoxI GGCC 2 cut(s) 108, 754
ApeKI GCWGC 4 cut(s) 279, 422, 915, 955
ApoI RAATTY 1 cut(s) 452
Asp718I GGTACC 1 cut(s) 566
AspLEI GCGC 5 cut(s) 509, 772, 926, 988, 1003
AspS9I GGNCC 3 cut(s) 293, 600, 1123
AsuHPI GGTGA 3 cut(s) 28, 222, 949
AsuII TTCGAA 1 cut(s) 116
AsuNHI GCTAGC 1 cut(s) 542
AvaI CYCGRG 1 cut(s) 968
AvaII GGWCC 3 cut(s) 293, 600, 1123
BalI TGGCCA 2 cut(s) 110, 756
BanI GGYRCC 1 cut(s) 566
BanII GRGCYC 3 cut(s) 803, 830, 951
BbrPI CACGTG 1 cut(s) 1017
BbsI GAAGAC 2 cut(s) 471, 873
Bbv12I GWGCWC 3 cut(s) 778, 830, 951
BbvI GCAGC 4 cut(s) 266, 434, 927, 967
BccI CCATC 5 cut(s) 41, 395, 487, 734, 887
BcgI CGANNNNNNTGC 4 cut(s) 6, 40, 999, 1033
BciT130I CCWGG 1 cut(s) 378
BclI TGATCA 2 cut(s) 343, 523
BcoDI GTCTC 2 cut(s) 236, 777
BfaI CTAG 2 cut(s) 174, 543
BfmI CTRYAG 1 cut(s) 15
BfoI RGCGCY 3 cut(s) 510, 989, 1004
BfrI CTTAAG 1 cut(s) 1141
BisI GCNGC 4 cut(s) 280, 423, 916, 956
BlsI GCNGC 4 cut(s) 281, 424, 917, 957
Bme1390I CCNGG 1 cut(s) 378
Bme18I GGWCC 3 cut(s) 293, 600, 1123
BmeT110I CYCGRG 1 cut(s) 968
BmgT120I GGNCC 3 cut(s) 293, 600, 1123
BmiI GGNNCC 1 cut(s) 568
BmrFI CCNGG 1 cut(s) 378
BmsI GCATC 2 cut(s) 409, 529
BmtI GCTAGC 1 cut(s) 546
BpiI GAAGAC 2 cut(s) 471, 873
BpmI CTGGAG 1 cut(s) 989
Bpu14I TTCGAA 1 cut(s) 116
BpuEI CTTGAG 2 cut(s) 844, 1115
BsaAI YACGTR 1 cut(s) 1017
BsaJI CCNNGG 1 cut(s) 377
BsaWI WCCGGW 3 cut(s) 38, 131, 563
BsaXI ACNNNNNCTCC 4 cut(s) 302, 332, 545, 575
Bsc4I CCNNNNNNNGG 2 cut(s) 377, 1132
Bse118I RCCGGY 1 cut(s) 232
Bse1I ACTGG 2 cut(s) 791, 919
BseBI CCWGG 1 cut(s) 378
BseDI CCNNGG 1 cut(s) 377
BseGI GGATG 4 cut(s) 52, 319, 444, 910
BseLI CCNNNNNNNGG 2 cut(s) 377, 1132
BseMII CTCAG 3 cut(s) 240, 276, 561
BseNI ACTGG 2 cut(s) 791, 919
BseXI GCAGC 4 cut(s) 266, 434, 927, 967
BseYI CCCAGC 2 cut(s) 620, 721
BsgI GTGCAG 1 cut(s) 676
Bsh1236I CGCG 1 cut(s) 772
BshFI GGCC 2 cut(s) 110, 756
BshNI GGYRCC 1 cut(s) 566
BsiHKAI GWGCWC 3 cut(s) 778, 830, 951
BsiHKCI CYCGRG 1 cut(s) 968
BsiSI CCGG 4 cut(s) 39, 132, 233, 564
BslFI GGGAC 1 cut(s) 638
BslI CCNNNNNNNGG 2 cut(s) 377, 1132
BsmAI GTCTC 2 cut(s) 236, 777
BsmFI GGGAC 1 cut(s) 638
BsmI GAATGC 1 cut(s) 78
BsnI GGCC 2 cut(s) 110, 756
BsoBI CYCGRG 1 cut(s) 968
Bsp119I TTCGAA 1 cut(s) 116
Bsp1286I GDGCHC 4 cut(s) 778, 803, 830, 951
Bsp143I GATC 8 cut(s) 78, 88, 343, 523, 588, 676, 964, 1057
BspACI CCGC 1 cut(s) 649
BspANI GGCC 2 cut(s) 110, 756
BspCNI CTCAG 3 cut(s) 241, 277, 560
BspFNI CGCG 1 cut(s) 772
BspLI GGNNCC 1 cut(s) 568
BspMAI CTGCAG 1 cut(s) 19
BspOI GCTAGC 1 cut(s) 546
BspPI GGATC 2 cut(s) 959, 1052
BspT104I TTCGAA 1 cut(s) 116
BspT107I GGYRCC 1 cut(s) 566
BspTI CTTAAG 1 cut(s) 1141
BsrFI RCCGGY 1 cut(s) 232
BsrI ACTGG 2 cut(s) 791, 919
BssAI RCCGGY 1 cut(s) 232
BssECI CCNNGG 1 cut(s) 377
BssMI GATC 8 cut(s) 78, 88, 343, 523, 588, 676, 964, 1057
Bst2UI CCWGG 1 cut(s) 378
Bst4CI ACNGT 3 cut(s) 303, 629, 1033
BstAFI CTTAAG 1 cut(s) 1141
BstAPI GCANNNNNTGC 1 cut(s) 921
BstBAI YACGTR 1 cut(s) 1017
BstBI TTCGAA 1 cut(s) 116
BstC8I GCNNGC 3 cut(s) 544, 647, 774
BstDEI CTNAG 4 cut(s) 249, 285, 547, 592
BstENI CCTNNNNNAGG 1 cut(s) 1130
BstF5I GGATG 4 cut(s) 52, 319, 444, 910
BstFNI CGCG 1 cut(s) 772
BstH2I RGCGCY 3 cut(s) 510, 989, 1004
BstHHI GCGC 5 cut(s) 509, 772, 926, 988, 1003
BstKTI GATC 8 cut(s) 81, 91, 346, 526, 591, 679, 967, 1060
BstMAI GTCTC 2 cut(s) 236, 777
BstMBI GATC 8 cut(s) 78, 88, 343, 523, 588, 676, 964, 1057
BstMWI GCNNNNNNNGC 6 cut(s) 527, 581, 825, 894, 921, 955
BstNI CCWGG 1 cut(s) 378
BstSCI CCNGG 1 cut(s) 376
BstSFI CTRYAG 1 cut(s) 15
BstUI CGCG 1 cut(s) 772
BstV1I GCAGC 4 cut(s) 266, 434, 927, 967
BstV2I GAAGAC 2 cut(s) 471, 873
BstX2I RGATCY 1 cut(s) 1057
BstYI RGATCY 1 cut(s) 1057
BsuRI GGCC 2 cut(s) 110, 756
BtsCI GGATG 4 cut(s) 52, 319, 444, 910
BtsI GCAGTG 1 cut(s) 570
BtsIMutI CAGTG 3 cut(s) 570, 926, 1029
Cac8I GCNNGC 3 cut(s) 544, 647, 774
CaiI CAGNNNCTG 1 cut(s) 14
CfoI GCGC 5 cut(s) 509, 772, 926, 988, 1003
Cfr10I RCCGGY 1 cut(s) 232
Cfr13I GGNCC 3 cut(s) 293, 600, 1123
CseI GACGC 2 cut(s) 110, 414
Csp6I GTAC 3 cut(s) 304, 319, 567
CviAII CATG 6 cut(s) 82, 307, 341, 527, 604, 952
CviQI GTAC 3 cut(s) 304, 319, 567
DdeI CTNAG 4 cut(s) 249, 285, 547, 592
DpnI GATC 8 cut(s) 80, 90, 345, 525, 590, 678, 966, 1059
DpnII GATC 8 cut(s) 78, 88, 343, 523, 588, 676, 964, 1057
EaeI YGGCCR 2 cut(s) 108, 754
Ecl136II GAGCTC 2 cut(s) 828, 949
Eco24I GRGCYC 3 cut(s) 803, 830, 951
Eco32I GATATC 1 cut(s) 22
Eco47I GGWCC 3 cut(s) 293, 600, 1123
Eco47III AGCGCT 3 cut(s) 508, 987, 1002
Eco53kI GAGCTC 2 cut(s) 828, 949
Eco57I CTGAAG 1 cut(s) 1134
Eco72I CACGTG 1 cut(s) 1017
Eco88I CYCGRG 1 cut(s) 968
EcoICRI GAGCTC 2 cut(s) 828, 949
EcoNI CCTNNNNNAGG 1 cut(s) 1130
EcoO109I RGGNCCY 1 cut(s) 293
EcoRII CCWGG 1 cut(s) 376
EcoRV GATATC 1 cut(s) 22
EcoT22I ATGCAT 1 cut(s) 184
EcoT38I GRGCYC 3 cut(s) 803, 830, 951
FaeI CATG 6 cut(s) 85, 310, 344, 530, 607, 955
FalI AAGNNNNNCTT 1 cut(s) 1124
FaqI GGGAC 1 cut(s) 638
FatI CATG 6 cut(s) 81, 306, 340, 526, 603, 951
FauI CCCGC 1 cut(s) 642
FbaI TGATCA 2 cut(s) 343, 523
FblI GTMKAC 1 cut(s) 99
Fnu4HI GCNGC 4 cut(s) 280, 423, 916, 956
FokI GGATG 4 cut(s) 59, 326, 431, 917
FriOI GRGCYC 3 cut(s) 803, 830, 951
Fsp4HI GCNGC 4 cut(s) 280, 423, 916, 956
FspBI CTAG 2 cut(s) 174, 543
FspI TGCGCA 1 cut(s) 925
GlaI GCGC 5 cut(s) 508, 771, 925, 987, 1002
GluI GCNGC 4 cut(s) 280, 423, 916, 956
GsaI CCCAGC 2 cut(s) 624, 725
GsuI CTGGAG 1 cut(s) 989
HaeII RGCGCY 3 cut(s) 510, 989, 1004
HaeIII GGCC 2 cut(s) 110, 756
HapII CCGG 4 cut(s) 39, 132, 233, 564
HgaI GACGC 2 cut(s) 110, 414
HhaI GCGC 5 cut(s) 509, 772, 926, 988, 1003
Hin1II CATG 6 cut(s) 85, 310, 344, 530, 607, 955
Hin6I GCGC 5 cut(s) 507, 770, 924, 986, 1001
HinP1I GCGC 5 cut(s) 507, 770, 924, 986, 1001
HincII GTYRAC 3 cut(s) 100, 189, 553
HindII GTYRAC 3 cut(s) 100, 189, 553
HinfI GANTC 3 cut(s) 194, 200, 467
HpaII CCGG 4 cut(s) 39, 132, 233, 564
HphI GGTGA 3 cut(s) 28, 222, 949
Hpy166II GTNNAC 3 cut(s) 100, 189, 553
Hpy188I TCNGA 7 cut(s) 144, 199, 205, 286, 700, 832, 1054
Hpy188III TCNNGA 4 cut(s) 9, 273, 877, 968
Hpy8I GTNNAC 3 cut(s) 100, 189, 553
Hpy99I CGWCG 2 cut(s) 101, 104
HpyAV CCTTC 8 cut(s) 381, 511, 591, 648, 937, 1092, 1109, 1136
HpyCH4III ACNGT 3 cut(s) 303, 629, 1033
HpyCH4IV ACGT 2 cut(s) 96, 1016
HpyCH4V TGCA 7 cut(s) 17, 171, 182, 422, 536, 693, 955
HpyF10VI GCNNNNNNNGC 6 cut(s) 527, 581, 825, 894, 921, 955
HpyF3I CTNAG 4 cut(s) 249, 285, 547, 592
HpySE526I ACGT 2 cut(s) 96, 1016
Hsp92II CATG 6 cut(s) 85, 310, 344, 530, 607, 955
HspAI GCGC 5 cut(s) 507, 770, 924, 986, 1001
KpnI GGTACC 1 cut(s) 570
Ksp22I TGATCA 2 cut(s) 343, 523
Kzo9I GATC 8 cut(s) 78, 88, 343, 523, 588, 676, 964, 1057
LmnI GCTCC 4 cut(s) 833, 946, 1008, 1101
Lsp1109I GCAGC 4 cut(s) 266, 434, 927, 967
LweI GCATC 2 cut(s) 409, 529
MaeI CTAG 2 cut(s) 174, 543
MaeII ACGT 2 cut(s) 96, 1016
MaeIII GTNAC 3 cut(s) 34, 228, 928
MalI GATC 8 cut(s) 80, 90, 345, 525, 590, 678, 966, 1059
MboI GATC 8 cut(s) 78, 88, 343, 523, 588, 676, 964, 1057
MboII GAAGA 5 cut(s) 98, 476, 875, 878, 1067
MfeI CAATTG 1 cut(s) 660
MflI RGATCY 1 cut(s) 1057
MhlI GDGCHC 4 cut(s) 778, 803, 830, 951
MlsI TGGCCA 2 cut(s) 110, 756
MluCI AATT 7 cut(s) 112, 166, 357, 452, 660, 711, 849
MluNI TGGCCA 2 cut(s) 110, 756
MlyI GAGTC 1 cut(s) 461
MmeI TCCRAC 3 cut(s) 152, 822, 1101
Mox20I TGGCCA 2 cut(s) 110, 756
Mph1103I ATGCAT 1 cut(s) 184
MscI TGGCCA 2 cut(s) 110, 756
MseI TTAA 5 cut(s) 59, 450, 489, 707, 1142
MslI CAYNNNNRTG 1 cut(s) 411
Msp20I TGGCCA 2 cut(s) 110, 756
MspA1I CMGCKG 2 cut(s) 248, 1112
MspCI CTTAAG 1 cut(s) 1141
MspI CCGG 4 cut(s) 39, 132, 233, 564
MspR9I CCNGG 1 cut(s) 378
MunI CAATTG 1 cut(s) 660
Mva1269I GAATGC 1 cut(s) 78
MvaI CCWGG 1 cut(s) 378
MvnI CGCG 1 cut(s) 772
MwoI GCNNNNNNNGC 6 cut(s) 527, 581, 825, 894, 921, 955
NdeII GATC 8 cut(s) 78, 88, 343, 523, 588, 676, 964, 1057
NheI GCTAGC 1 cut(s) 542
NlaIII CATG 6 cut(s) 85, 310, 344, 530, 607, 955
NlaIV GGNNCC 1 cut(s) 568
NmuCI GTSAC 2 cut(s) 34, 228
NsbI TGCGCA 1 cut(s) 925
NsiI ATGCAT 1 cut(s) 184
NspV TTCGAA 1 cut(s) 116
PctI GAATGC 1 cut(s) 78
PfeI GAWTC 2 cut(s) 194, 200
PkrI GCNGC 4 cut(s) 281, 424, 917, 957
PleI GAGTC 1 cut(s) 461
PmaCI CACGTG 1 cut(s) 1017
PmlI CACGTG 1 cut(s) 1017
PpsI GAGTC 1 cut(s) 461
Ppu21I YACGTR 1 cut(s) 1017
PpuMI RGGWCCY 1 cut(s) 293
Psp124BI GAGCTC 2 cut(s) 830, 951
Psp5II RGGWCCY 1 cut(s) 293
Psp6I CCWGG 1 cut(s) 376
PspCI CACGTG 1 cut(s) 1017
PspFI CCCAGC 2 cut(s) 620, 721
PspGI CCWGG 1 cut(s) 376
PspN4I GGNNCC 1 cut(s) 568
PspPI GGNCC 3 cut(s) 293, 600, 1123
PspPPI RGGWCCY 1 cut(s) 293
PstI CTGCAG 1 cut(s) 19
PstNI CAGNNNCTG 1 cut(s) 14
PsuI RGATCY 1 cut(s) 1057
PvuII CAGCTG 2 cut(s) 248, 1112
RsaI GTAC 3 cut(s) 305, 320, 568
RsaNI GTAC 3 cut(s) 304, 319, 567
RseI CAYNNNNRTG 1 cut(s) 411
SacI GAGCTC 2 cut(s) 830, 951
SalI GTCGAC 1 cut(s) 98
SaqAI TTAA 5 cut(s) 59, 450, 489, 707, 1142
SatI GCNGC 4 cut(s) 280, 423, 916, 956
Sau3AI GATC 8 cut(s) 78, 88, 343, 523, 588, 676, 964, 1057
Sau96I GGNCC 3 cut(s) 293, 600, 1123
SchI GAGTC 1 cut(s) 461
ScrFI CCNGG 1 cut(s) 378
SduI GDGCHC 4 cut(s) 778, 803, 830, 951
SfaNI GCATC 2 cut(s) 409, 529
SfcI CTRYAG 1 cut(s) 15
SfuI TTCGAA 1 cut(s) 116
SgrDI CGTCGACG 1 cut(s) 98
SinI GGWCC 3 cut(s) 293, 600, 1123
SmiMI CAYNNNNRTG 1 cut(s) 411
SmlI CTYRAG 3 cut(s) 823, 1130, 1141
SmoI CTYRAG 3 cut(s) 823, 1130, 1141
Sse9I AATT 7 cut(s) 112, 166, 357, 452, 660, 711, 849
SsiI CCGC 1 cut(s) 649
SspMI CTAG 2 cut(s) 174, 543
SstI GAGCTC 2 cut(s) 830, 951
StyD4I CCNGG 1 cut(s) 376
TaaI ACNGT 3 cut(s) 303, 629, 1033
TaiI ACGT 2 cut(s) 99, 1019
TaqI TCGA 4 cut(s) 99, 116, 631, 803
TaqII GACCGA 1 cut(s) 206
TasI AATT 7 cut(s) 112, 166, 357, 452, 660, 711, 849
TatI WGTACW 2 cut(s) 303, 318
TfiI GAWTC 2 cut(s) 194, 200
Tru1I TTAA 5 cut(s) 59, 450, 489, 707, 1142
Tru9I TTAA 5 cut(s) 59, 450, 489, 707, 1142
TscAI CASTG 3 cut(s) 577, 926, 1036
TseFI GTSAC 2 cut(s) 34, 228
TseI GCWGC 4 cut(s) 279, 422, 915, 955
Tsp45I GTSAC 2 cut(s) 34, 228
TspDTI ATGAA 2 cut(s) 421, 445
TspGWI ACGGA 3 cut(s) 78, 257, 754
TspRI CASTG 3 cut(s) 577, 926, 1036
Vha464I CTTAAG 1 cut(s) 1141
VpaK11BI GGWCC 3 cut(s) 293, 600, 1123
XagI CCTNNNNNAGG 1 cut(s) 1130
XapI RAATTY 1 cut(s) 452
XmiI GTMKAC 1 cut(s) 99
XspI CTAG 2 cut(s) 174, 543
Zsp2I ATGCAT 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.