pycom02g08910

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0008979
Physical Location & Seq
Forward (+)
6156478 .. 6160258
3781 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g08910.1

Sequence Viewer

Length: 1647 bp
ATGGCGTCAGGAACCGCAGATATCTCGCCACAGGTGACCGGATTGGATGGTGGGTGCTTAAAGGGAGGTGGAATGCCGATCATGGAAGATCAAAACGTCGACGCTGGGGCCAATTCGAACCACATTCCTACTGGAAAGCCTCCGAGAAACCTCTCGGTTATGCGACATTGCACTAGCTATGCATTGTTGACCGAATCAGATTCAGATGTTGGAAGCATGGGATTAAAGTCGCCGGCAAGTGAGACAGCCGAATTCGTATTTGTATTCCGTTCAGGAAGCTGCTCGGAGAAAGGACCTAAACAGTACATGGAGGATGAGTATATTTGCGTAGATAATCTTAGCGATCGTATTGGTGAAATCGAAAACTTGCCTTCCCCTGGGGCATTTTATGGGGTCTTTGACGGACATAATGGTGTTGATGCAGCATCATTCATCAAAAGCAACATCCTTAAGTTCATCGTTGAAGACTCGCACTTTCCATCTGTCGTTAGAAAGGCAGTTAGAAGGGCTTTTGTGAAGGCTGATCATGCTTTTGCAGATGCTAGCTCTGTCGATAAGTCCTCCGGTACCACTGCCTTAACTGCCCTGATCTTAGGAAGGACCATGCTAATCGCTAATGCTGGGGACTGTCGAGCTGTATTAAGAATTGAAAAGCTGGGTGGTGTCATCTATGATGGCTACCTCAATGGCCAACTATCCGTGGCGCGTGCTCTTGGAGACTGGCATATCAAGGGCTCGAAAGGTTCAACTTGCCCCTTGAGCTCCGAGCCAGAGCTGGAGGAAATTGTCCTAACAGAAGAAGACGAGTTCTTGATAATTGGCTGTGATGGCTTGTGGGATGTGATGAGCAGCCAGTGTGCAGTCACAATGGTGAGGAAGGAGCTCATGCAGCACAATGATCCCGAGAGATGTTCAAAAGTGCTTGTCAAGGAGGCACTCCAACGGAACACCTGTGATAACCTCACTGTTGTCGTGGTCTGTTTCTCCGAAGATCCACCTCCTAAAACCGAAATTCCCAAATCCCACAAGAGGAGAAGCATTTCAGCTGAAGGGTTGGACCTTCTCAAGGGTGTCTTAAGCAACATTCCCACCAAGGCCTACTCATACTTGCTTCCCTCACCATTTCCCACAGAACCCAGACCTCCCAGAGCGAGTGTCTGTAGTCGAGTAGCAAAACCCATGGCAACCCTGAAGGAAATCCTTACCCGACGACCCGTGGCGGCGACGATCCGCCTCACAGTCCCGGCGGGAGCCGCTCGACCCGTAGCTCCGGTGGGTCCCGCTCTGGGTCAATACAGGCTCAACCTGATGGCCTTCTGCAAGGACTTCAACGCCCGGACCCAAAAGTACAAACCCGACACGCCCATGGCGGTGATCATCACCGCCTTCAAAGACAACACCTTCGAGTTCACGGTGAGGTCGCCGTCCGTCACCTGGTACTTGAAGAAGGCCGCGGGGATCGAGTCAGGCAGCAGCCGTCCCGGACACGCGGTGGCGTCGACGCTGTCGGTGAGGCACGTGTACGAGATCGCGAAGGTGAAGCAGTCCGACCCGTATTGCCAGTACATGCCATTGGAGTCCATTTGTAAGTCAGTTATTGGTACCGCTAATTCTATGGGAATTAAGGTTGTTAAGGAATTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

549

Amino Acids

59.26

Weight (kDa)

7.49

Isoelectric Point (pI)

52.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 91 - 214 1.1e-18 Protein phosphatase 2C
PP2C PF00481 215 - 320 8.2e-33 Protein phosphatase 2C
Ribosomal_L11_N PF03946 410 - 468 5.7e-21 Ribosomal protein L11, N-terminal domain
Ribosomal_L11 PF00298 474 - 543 2e-25 Ribosomal protein L11, RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 566, 1601
AccB1I GGYRCC 2 cut(s) 566, 1601
AccBSI CCGCTC 2 cut(s) 1256, 1283
AccI GTMKAC 2 cut(s) 99, 1499
AccII CGCG 4 cut(s) 706, 1454, 1490, 1532
AclWI GGATC 4 cut(s) 893, 986, 1222, 1466
AcoI YGGCCR 1 cut(s) 688
AcsI RAATTY 2 cut(s) 251, 1011
AcuI CTGAAG 2 cut(s) 1068, 1211
AcvI CACGTG 1 cut(s) 1519
AcyI GRCGYC 2 cut(s) 5, 1496
AdeI CACNNNGTG 1 cut(s) 1492
AfaI GTAC 7 cut(s) 305, 568, 1349, 1439, 1523, 1565, 1603
AfiI CCNNNNNNNGG 5 cut(s) 377, 1029, 1066, 1286, 1434
AflII CTTAAG 2 cut(s) 449, 1075
AflIII ACRYGT 1 cut(s) 1518
AgsI TTSAA 7 cut(s) 464, 650, 747, 915, 1330, 1390, 1444
AjnI CCWGG 2 cut(s) 376, 1433
AleI CACNNNNGTG 1 cut(s) 869
Alw21I GWGCWC 3 cut(s) 712, 764, 885
Alw26I GTCTC 2 cut(s) 236, 711
AlwI GGATC 4 cut(s) 893, 986, 1222, 1466
Ama87I CYCGRG 1 cut(s) 902
AoxI GGCC 5 cut(s) 108, 688, 1095, 1311, 1449
ApeKI GCWGC 6 cut(s) 279, 422, 849, 889, 1470, 1473
ApoI RAATTY 2 cut(s) 251, 1011
ArsI GACNNNNNNTTYG 2 cut(s) 1385, 1417
Asp700I GAANNNNTTC 1 cut(s) 1039
Asp718I GGTACC 2 cut(s) 566, 1601
AspLEI GCGC 1 cut(s) 706
AspS9I GGNCC 6 cut(s) 108, 293, 600, 1057, 1277, 1338
AsuC2I CCSGG 3 cut(s) 1244, 1336, 1482
AsuII TTCGAA 1 cut(s) 116
AsuNHI GCTAGC 1 cut(s) 542
AvaI CYCGRG 1 cut(s) 902
AvaII GGWCC 5 cut(s) 293, 600, 1057, 1277, 1338
BalI TGGCCA 1 cut(s) 690
BanI GGYRCC 2 cut(s) 566, 1601
BanII GRGCYC 3 cut(s) 737, 764, 885
BbrPI CACGTG 1 cut(s) 1519
BbsI GAAGAC 2 cut(s) 471, 807
Bbv12I GWGCWC 3 cut(s) 712, 764, 885
BbvI GCAGC 6 cut(s) 266, 434, 861, 901, 1482, 1485
BccI CCATC 5 cut(s) 41, 487, 668, 821, 1303
BceAI ACGGC 2 cut(s) 1408, 1461
BcgI CGANNNNNNTGC 2 cut(s) 6, 40
BciT130I CCWGG 2 cut(s) 378, 1435
BclI TGATCA 2 cut(s) 523, 1374
BcnI CCSGG 3 cut(s) 1244, 1336, 1482
BcoDI GTCTC 2 cut(s) 236, 711
BfaI CTAG 2 cut(s) 174, 543
BfmI CTRYAG 1 cut(s) 1159
BfrI CTTAAG 2 cut(s) 449, 1075
BisI GCNGC 9 cut(s) 280, 423, 850, 890, 1221, 1254, 1452, 1471, 1474
BlsI GCNGC 9 cut(s) 281, 424, 851, 891, 1222, 1255, 1453, 1472, 1475
Bme1390I CCNGG 5 cut(s) 378, 1244, 1336, 1435, 1482
Bme18I GGWCC 5 cut(s) 293, 600, 1057, 1277, 1338
BmeT110I CYCGRG 1 cut(s) 902
BmgT120I GGNCC 6 cut(s) 108, 293, 600, 1057, 1277, 1338
BmiI GGNNCC 8 cut(s) 13, 109, 568, 1252, 1278, 1279, 1340, 1603
BmrFI CCNGG 5 cut(s) 378, 1244, 1336, 1435, 1482
BmsI GCATC 3 cut(s) 409, 434, 529
BmtI GCTAGC 1 cut(s) 546
BpiI GAAGAC 2 cut(s) 471, 807
BpmI CTGGAG 1 cut(s) 797
Bpu14I TTCGAA 1 cut(s) 116
BpuEI CTTGAG 2 cut(s) 778, 1049
BpuMI CCSGG 3 cut(s) 1244, 1336, 1482
BsaAI YACGTR 1 cut(s) 1519
BsaHI GRCGYC 2 cut(s) 5, 1496
BsaJI CCNNGG 8 cut(s) 376, 377, 699, 1092, 1179, 1215, 1365, 1452
BsaWI WCCGGW 3 cut(s) 38, 563, 1270
BsaXI ACNNNNNCTCC 2 cut(s) 302, 332
Bsc4I CCNNNNNNNGG 5 cut(s) 377, 1029, 1066, 1286, 1434
Bse118I RCCGGY 1 cut(s) 232
Bse1I ACTGG 4 cut(s) 136, 725, 853, 1561
Bse3DI GCAATG 1 cut(s) 166
BseBI CCWGG 2 cut(s) 378, 1435
BseDI CCNNGG 8 cut(s) 376, 377, 699, 1092, 1179, 1215, 1365, 1452
BseGI GGATG 4 cut(s) 52, 319, 444, 844
BseLI CCNNNNNNNGG 5 cut(s) 377, 1029, 1066, 1286, 1434
BseMI GCAATG 1 cut(s) 166
BseNI ACTGG 4 cut(s) 136, 725, 853, 1561
BseRI GAGGAG 1 cut(s) 1045
BseXI GCAGC 6 cut(s) 266, 434, 861, 901, 1482, 1485
BseYI CCCAGC 3 cut(s) 104, 620, 655
BsgI GTGCAG 1 cut(s) 879
Bsh1236I CGCG 4 cut(s) 706, 1454, 1490, 1532
Bsh1285I CGRYCG 1 cut(s) 346
BshFI GGCC 5 cut(s) 110, 690, 1097, 1313, 1451
BshNI GGYRCC 2 cut(s) 566, 1601
BsiEI CGRYCG 1 cut(s) 346
BsiHKAI GWGCWC 3 cut(s) 712, 764, 885
BsiHKCI CYCGRG 1 cut(s) 902
BsiSI CCGG 7 cut(s) 39, 233, 564, 1244, 1271, 1336, 1482
BslFI GGGAC 4 cut(s) 638, 1226, 1263, 1464
BslI CCNNNNNNNGG 5 cut(s) 377, 1029, 1066, 1286, 1434
BsmAI GTCTC 2 cut(s) 236, 711
BsmFI GGGAC 4 cut(s) 638, 1226, 1263, 1464
BsmI GAATGC 1 cut(s) 78
BsnI GGCC 5 cut(s) 110, 690, 1097, 1313, 1451
BsoBI CYCGRG 1 cut(s) 902
Bsp119I TTCGAA 1 cut(s) 116
Bsp1286I GDGCHC 4 cut(s) 712, 737, 764, 885
Bsp19I CCATGG 2 cut(s) 1179, 1365
Bsp68I TCGCGA 1 cut(s) 1532
BspANI GGCC 5 cut(s) 110, 690, 1097, 1313, 1451
BspFNI CGCG 4 cut(s) 706, 1454, 1490, 1532
BspLI GGNNCC 8 cut(s) 13, 109, 568, 1252, 1278, 1279, 1340, 1603
BspOI GCTAGC 1 cut(s) 546
BspPI GGATC 4 cut(s) 893, 986, 1222, 1466
BspT104I TTCGAA 1 cut(s) 116
BspT107I GGYRCC 2 cut(s) 566, 1601
BspTI CTTAAG 2 cut(s) 449, 1075
BsrBI CCGCTC 2 cut(s) 1256, 1283
BsrDI GCAATG 1 cut(s) 166
BsrFI RCCGGY 1 cut(s) 232
BsrI ACTGG 4 cut(s) 136, 725, 853, 1561
BssAI RCCGGY 1 cut(s) 232
BssECI CCNNGG 8 cut(s) 376, 377, 699, 1092, 1179, 1215, 1365, 1452
BssNI GRCGYC 2 cut(s) 5, 1496
BssT1I CCWWGG 3 cut(s) 1092, 1179, 1365
Bst2UI CCWGG 2 cut(s) 378, 1435
Bst4CI ACNGT 5 cut(s) 303, 629, 967, 1240, 1414
BstACI GRCGYC 2 cut(s) 5, 1496
BstAFI CTTAAG 2 cut(s) 449, 1075
BstBAI YACGTR 1 cut(s) 1519
BstBI TTCGAA 1 cut(s) 116
BstC8I GCNNGC 3 cut(s) 234, 544, 708
BstDEI CTNAG 2 cut(s) 338, 592
BstDSI CCRYGG 5 cut(s) 699, 1179, 1215, 1365, 1452
BstEII GGTNACC 1 cut(s) 34
BstENI CCTNNNNNAGG 1 cut(s) 1064
BstF5I GGATG 4 cut(s) 52, 319, 444, 844
BstFNI CGCG 4 cut(s) 706, 1454, 1490, 1532
BstHHI GCGC 1 cut(s) 706
BstMAI GTCTC 2 cut(s) 236, 711
BstMCI CGRYCG 1 cut(s) 346
BstMWI GCNNNNNNNGC 6 cut(s) 527, 581, 759, 828, 889, 1253
BstNI CCWGG 2 cut(s) 378, 1435
BstNSI RCATGY 1 cut(s) 1570
BstPI GGTNACC 1 cut(s) 34
BstSCI CCNGG 5 cut(s) 376, 1242, 1334, 1433, 1480
BstSFI CTRYAG 1 cut(s) 1159
BstUI CGCG 4 cut(s) 706, 1454, 1490, 1532
BstV1I GCAGC 6 cut(s) 266, 434, 861, 901, 1482, 1485
BstV2I GAAGAC 2 cut(s) 471, 807
BstX2I RGATCY 1 cut(s) 991
BstYI RGATCY 1 cut(s) 991
BsuRI GGCC 5 cut(s) 110, 690, 1097, 1313, 1451
BtgI CCRYGG 5 cut(s) 699, 1179, 1215, 1365, 1452
BtsCI GGATG 4 cut(s) 52, 319, 444, 844
BtsI GCAGTG 1 cut(s) 570
BtsIMutI CAGTG 3 cut(s) 570, 860, 963
BtuMI TCGCGA 1 cut(s) 1532
Cac8I GCNNGC 3 cut(s) 234, 544, 708
CfoI GCGC 1 cut(s) 706
Cfr10I RCCGGY 1 cut(s) 232
Cfr13I GGNCC 6 cut(s) 108, 293, 600, 1057, 1277, 1338
Cfr42I CCGCGG 1 cut(s) 1455
CseI GACGC 3 cut(s) 110, 1485, 1510
CsiI ACCWGGT 1 cut(s) 1433
Csp6I GTAC 7 cut(s) 304, 567, 1348, 1438, 1522, 1564, 1602
CviAII CATG 9 cut(s) 82, 217, 307, 527, 604, 886, 1180, 1366, 1567
CviQI GTAC 7 cut(s) 304, 567, 1348, 1438, 1522, 1564, 1602
DdeI CTNAG 2 cut(s) 338, 592
DraIII CACNNNGTG 1 cut(s) 1492
EaeI YGGCCR 1 cut(s) 688
EciI GGCGGA 1 cut(s) 1220
Ecl136II GAGCTC 2 cut(s) 762, 883
Eco130I CCWWGG 3 cut(s) 1092, 1179, 1365
Eco147I AGGCCT 1 cut(s) 1097
Eco24I GRGCYC 3 cut(s) 737, 764, 885
Eco32I GATATC 1 cut(s) 22
Eco47I GGWCC 5 cut(s) 293, 600, 1057, 1277, 1338
Eco53kI GAGCTC 2 cut(s) 762, 883
Eco57I CTGAAG 2 cut(s) 1068, 1211
Eco72I CACGTG 1 cut(s) 1519
Eco88I CYCGRG 1 cut(s) 902
Eco91I GGTNACC 1 cut(s) 34
EcoICRI GAGCTC 2 cut(s) 762, 883
EcoNI CCTNNNNNAGG 1 cut(s) 1064
EcoO109I RGGNCCY 2 cut(s) 293, 1277
EcoO65I GGTNACC 1 cut(s) 34
EcoRI GAATTC 1 cut(s) 251
EcoRII CCWGG 2 cut(s) 376, 1433
EcoRV GATATC 1 cut(s) 22
EcoT14I CCWWGG 3 cut(s) 1092, 1179, 1365
EcoT22I ATGCAT 1 cut(s) 184
EcoT38I GRGCYC 3 cut(s) 737, 764, 885
ErhI CCWWGG 3 cut(s) 1092, 1179, 1365
FaeI CATG 9 cut(s) 85, 220, 310, 530, 607, 889, 1183, 1369, 1570
FalI AAGNNNNNCTT 2 cut(s) 1058, 1090
FaqI GGGAC 4 cut(s) 638, 1226, 1263, 1464
FatI CATG 9 cut(s) 81, 216, 306, 526, 603, 885, 1179, 1365, 1566
FauI CCCGC 3 cut(s) 1240, 1288, 1447
FbaI TGATCA 2 cut(s) 523, 1374
FblI GTMKAC 2 cut(s) 99, 1499
Fnu4HI GCNGC 9 cut(s) 280, 423, 850, 890, 1221, 1254, 1452, 1471, 1474
FokI GGATG 4 cut(s) 59, 326, 431, 851
FriOI GRGCYC 3 cut(s) 737, 764, 885
Fsp4HI GCNGC 9 cut(s) 280, 423, 850, 890, 1221, 1254, 1452, 1471, 1474
FspBI CTAG 2 cut(s) 174, 543
GlaI GCGC 1 cut(s) 705
GluI GCNGC 9 cut(s) 280, 423, 850, 890, 1221, 1254, 1452, 1471, 1474
GsaI CCCAGC 3 cut(s) 108, 624, 659
GsuI CTGGAG 1 cut(s) 797
HaeIII GGCC 5 cut(s) 110, 690, 1097, 1313, 1451
HapII CCGG 7 cut(s) 39, 233, 564, 1244, 1271, 1336, 1482
HgaI GACGC 3 cut(s) 110, 1485, 1510
HhaI GCGC 1 cut(s) 706
Hin1I GRCGYC 2 cut(s) 5, 1496
Hin1II CATG 9 cut(s) 85, 220, 310, 530, 607, 889, 1183, 1369, 1570
Hin6I GCGC 1 cut(s) 704
HinP1I GCGC 1 cut(s) 704
HincII GTYRAC 3 cut(s) 100, 189, 1500
HindII GTYRAC 3 cut(s) 100, 189, 1500
HinfI GANTC 5 cut(s) 194, 200, 467, 1463, 1577
HpaII CCGG 7 cut(s) 39, 233, 564, 1244, 1271, 1336, 1482
Hpy166II GTNNAC 5 cut(s) 100, 189, 1410, 1500, 1522
Hpy188I TCNGA 7 cut(s) 144, 199, 205, 286, 766, 988, 1549
Hpy188III TCNNGA 5 cut(s) 9, 273, 811, 902, 1531
Hpy8I GTNNAC 5 cut(s) 100, 189, 1410, 1500, 1522
Hpy99I CGWCG 6 cut(s) 101, 104, 1212, 1228, 1501, 1504
HpyCH4III ACNGT 5 cut(s) 303, 629, 967, 1240, 1414
HpyCH4IV ACGT 2 cut(s) 96, 1518
HpyCH4V TGCA 7 cut(s) 171, 182, 422, 536, 860, 889, 1320
HpyF10VI GCNNNNNNNGC 6 cut(s) 527, 581, 759, 828, 889, 1253
HpyF3I CTNAG 2 cut(s) 338, 592
HpySE526I ACGT 2 cut(s) 96, 1518
Hsp92I GRCGYC 2 cut(s) 5, 1496
Hsp92II CATG 9 cut(s) 85, 220, 310, 530, 607, 889, 1183, 1369, 1570
HspAI GCGC 1 cut(s) 704
KflI GGGWCCC 1 cut(s) 1277
KpnI GGTACC 2 cut(s) 570, 1605
KroI GCCGGC 1 cut(s) 232
KroNI GCCGGC 1 cut(s) 234
Ksp22I TGATCA 2 cut(s) 523, 1374
KspI CCGCGG 1 cut(s) 1455
LmnI GCTCC 4 cut(s) 767, 880, 1250, 1273
Lsp1109I GCAGC 6 cut(s) 266, 434, 861, 901, 1482, 1485
LweI GCATC 3 cut(s) 409, 434, 529
MabI ACCWGGT 1 cut(s) 1433
MaeI CTAG 2 cut(s) 174, 543
MaeII ACGT 2 cut(s) 96, 1518
MaeIII GTNAC 3 cut(s) 34, 862, 1429
MbiI CCGCTC 2 cut(s) 1256, 1283
MboII GAAGA 6 cut(s) 98, 476, 809, 812, 1001, 1456
MflI RGATCY 1 cut(s) 991
MhlI GDGCHC 4 cut(s) 712, 737, 764, 885
MlsI TGGCCA 1 cut(s) 690
MluCI AATT 9 cut(s) 112, 251, 645, 783, 816, 1011, 1609, 1620, 1637
MluNI TGGCCA 1 cut(s) 690
MlyI GAGTC 3 cut(s) 461, 1472, 1586
MmeI TCCRAC 4 cut(s) 190, 964, 1035, 1572
Mox20I TGGCCA 1 cut(s) 690
Mph1103I ATGCAT 1 cut(s) 184
MroNI GCCGGC 1 cut(s) 232
MroXI GAANNNNTTC 1 cut(s) 1039
MscI TGGCCA 1 cut(s) 690
MseI TTAA 8 cut(s) 59, 224, 450, 578, 641, 1076, 1623, 1632
MslI CAYNNNNRTG 4 cut(s) 411, 869, 1364, 1370
Msp20I TGGCCA 1 cut(s) 690
MspA1I CMGCKG 2 cut(s) 1046, 1454
MspCI CTTAAG 2 cut(s) 449, 1075
MspI CCGG 7 cut(s) 39, 233, 564, 1244, 1271, 1336, 1482
MspR9I CCNGG 5 cut(s) 378, 1244, 1336, 1435, 1482
Mva1269I GAATGC 1 cut(s) 78
MvaI CCWGG 2 cut(s) 378, 1435
MvnI CGCG 4 cut(s) 706, 1454, 1490, 1532
MwoI GCNNNNNNNGC 6 cut(s) 527, 581, 759, 828, 889, 1253
NaeI GCCGGC 1 cut(s) 234
NciI CCSGG 3 cut(s) 1244, 1336, 1482
NcoI CCATGG 2 cut(s) 1179, 1365
NgoMIV GCCGGC 1 cut(s) 232
NheI GCTAGC 1 cut(s) 542
NlaIII CATG 9 cut(s) 85, 220, 310, 530, 607, 889, 1183, 1369, 1570
NlaIV GGNNCC 8 cut(s) 13, 109, 568, 1252, 1278, 1279, 1340, 1603
NmuCI GTSAC 3 cut(s) 34, 862, 1429
NruI TCGCGA 1 cut(s) 1532
NsiI ATGCAT 1 cut(s) 184
NspI RCATGY 1 cut(s) 1570
NspV TTCGAA 1 cut(s) 116
OliI CACNNNNGTG 1 cut(s) 869
PasI CCCWGGG 1 cut(s) 377
PceI AGGCCT 1 cut(s) 1097
PctI GAATGC 1 cut(s) 78
PdiI GCCGGC 1 cut(s) 234
PdmI GAANNNNTTC 1 cut(s) 1039
PfeI GAWTC 2 cut(s) 194, 200
PflFI GACNNNGTC 1 cut(s) 1504
PfoI TCCNGGA 1 cut(s) 1480
PkrI GCNGC 9 cut(s) 281, 424, 851, 891, 1222, 1255, 1453, 1472, 1475
Ple19I CGATCG 1 cut(s) 346
PleI GAGTC 3 cut(s) 461, 1471, 1585
PmaCI CACGTG 1 cut(s) 1519
PmlI CACGTG 1 cut(s) 1519
PpsI GAGTC 3 cut(s) 461, 1471, 1585
Ppu21I YACGTR 1 cut(s) 1519
PpuMI RGGWCCY 2 cut(s) 293, 1277
Psp124BI GAGCTC 2 cut(s) 764, 885
Psp5II RGGWCCY 2 cut(s) 293, 1277
Psp6I CCWGG 2 cut(s) 376, 1433
PspCI CACGTG 1 cut(s) 1519
PspEI GGTNACC 1 cut(s) 34
PspFI CCCAGC 3 cut(s) 104, 620, 655
PspGI CCWGG 2 cut(s) 376, 1433
PspN4I GGNNCC 8 cut(s) 13, 109, 568, 1252, 1278, 1279, 1340, 1603
PspPI GGNCC 6 cut(s) 108, 293, 600, 1057, 1277, 1338
PspPPI RGGWCCY 2 cut(s) 293, 1277
PsuI RGATCY 1 cut(s) 991
PsyI GACNNNGTC 1 cut(s) 1504
PvuI CGATCG 1 cut(s) 346
PvuII CAGCTG 1 cut(s) 1046
RruI TCGCGA 1 cut(s) 1532
RsaI GTAC 7 cut(s) 305, 568, 1349, 1439, 1523, 1565, 1603
RsaNI GTAC 7 cut(s) 304, 567, 1348, 1438, 1522, 1564, 1602
RseI CAYNNNNRTG 4 cut(s) 411, 869, 1364, 1370
SacI GAGCTC 2 cut(s) 764, 885
SacII CCGCGG 1 cut(s) 1455
SalI GTCGAC 2 cut(s) 98, 1498
SaqAI TTAA 8 cut(s) 59, 224, 450, 578, 641, 1076, 1623, 1632
SatI GCNGC 9 cut(s) 280, 423, 850, 890, 1221, 1254, 1452, 1471, 1474
Sau96I GGNCC 6 cut(s) 108, 293, 600, 1057, 1277, 1338
SchI GAGTC 3 cut(s) 461, 1472, 1586
ScrFI CCNGG 5 cut(s) 378, 1244, 1336, 1435, 1482
SduI GDGCHC 4 cut(s) 712, 737, 764, 885
SexAI ACCWGGT 1 cut(s) 1433
SfaNI GCATC 3 cut(s) 409, 434, 529
SfcI CTRYAG 1 cut(s) 1159
Sfr303I CCGCGG 1 cut(s) 1455
SfuI TTCGAA 1 cut(s) 116
SgrBI CCGCGG 1 cut(s) 1455
SgrDI CGTCGACG 2 cut(s) 98, 1498
SinI GGWCC 5 cut(s) 293, 600, 1057, 1277, 1338
SmiMI CAYNNNNRTG 4 cut(s) 411, 869, 1364, 1370
SmlI CTYRAG 4 cut(s) 449, 757, 1064, 1075
SmoI CTYRAG 4 cut(s) 449, 757, 1064, 1075
Sse9I AATT 9 cut(s) 112, 251, 645, 783, 816, 1011, 1609, 1620, 1637
SseBI AGGCCT 1 cut(s) 1097
SspMI CTAG 2 cut(s) 174, 543
SstI GAGCTC 2 cut(s) 764, 885
StuI AGGCCT 1 cut(s) 1097
StyD4I CCNGG 5 cut(s) 376, 1242, 1334, 1433, 1480
StyI CCWWGG 3 cut(s) 1092, 1179, 1365
TaaI ACNGT 5 cut(s) 303, 629, 967, 1240, 1414
TaiI ACGT 2 cut(s) 99, 1521
TaqII GACCGA 1 cut(s) 206
TasI AATT 9 cut(s) 112, 251, 645, 783, 816, 1011, 1609, 1620, 1637
TatI WGTACW 3 cut(s) 303, 1347, 1563
TauI GCSGC 3 cut(s) 1223, 1256, 1454
TfiI GAWTC 2 cut(s) 194, 200
Tru1I TTAA 8 cut(s) 59, 224, 450, 578, 641, 1076, 1623, 1632
Tru9I TTAA 8 cut(s) 59, 224, 450, 578, 641, 1076, 1623, 1632
TscAI CASTG 3 cut(s) 577, 860, 970
TseFI GTSAC 3 cut(s) 34, 862, 1429
TseI GCWGC 6 cut(s) 279, 422, 849, 889, 1470, 1473
Tsp45I GTSAC 3 cut(s) 34, 862, 1429
TspDTI ATGAA 2 cut(s) 421, 445
TspGWI ACGGA 5 cut(s) 257, 417, 688, 958, 1417
TspRI CASTG 3 cut(s) 577, 860, 970
Tth111I GACNNNGTC 1 cut(s) 1504
Vha464I CTTAAG 2 cut(s) 449, 1075
VpaK11BI GGWCC 5 cut(s) 293, 600, 1057, 1277, 1338
XagI CCTNNNNNAGG 1 cut(s) 1064
XapI RAATTY 2 cut(s) 251, 1011
XceI RCATGY 1 cut(s) 1570
XcmI CCANNNNNNNNNTGG 1 cut(s) 128
XmiI GTMKAC 2 cut(s) 99, 1499
XmnI GAANNNNTTC 1 cut(s) 1039
XspI CTAG 2 cut(s) 174, 543
Zsp2I ATGCAT 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.