Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0014635

Physical Location & Seq

Genomic Coordinates Forward (+)

19587857 .. 19593674

Gene Structure 5818 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1237900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1251 bp

ATGTGTTTTGTTAATCCGTTTGACAAGACAACATTAGTTACAAAGTTGAAAATCTGCAGAGAGCACTCGAAGATTTTGGGGTGTGACTACATATGTTTTTGTATATTAGGGAATTTGAAAGAAGAGAAAATAATGTTGAGGCGCTTCCAGTTGGATGAACTAGTGAAGGCTACTAAGAATTTCAGCAGTGAGTGCTTGTTGGGTTCCGGTGCTTTTGCAAATGTCTTCAAGGGGACCTTTGATGATTTAGGAACCCTAGCCATTAAGCGAACCGCCAATTCATTTCATAGTGTCGAAGAATTCAGAAATGAAGTGAGGTTACTTTCTAGGGTCAAGCACAGAAACCTTGTACGTTTGGTTGGATATTGTGAAGAAGGATCATCTGGAAGAAAATGGAGGAGCTTAACTTGGAGGCAAAGAGTCAACATAGCCATTGGAGCAGCAAAAGGCATTGCTCATTTACATGAAGGAGTAAACCCGAACATAATCCACCGCGATATAAAGCTTAATAACATCTTGATAGGAGATGAATTTGAAGCCAAGGTTTCAGATTTTGGACTTGTTAGGTCAGGACCTACTGGGGATCAATCACATGTCAGCAGCCAAATCAAAGGAACTCCAGGATACCTAGACCATGCCTATTGTTCAAGTTTCCATTTGACGCCGTTTAGCGATGTCTATAGTTTTGGTGTGATACTTCTGCAACTTGTTTCTTCTCGGCCAGTGGTTGATTCAACCGAGCACCGCCCCAATCAACATATCTTTGACTGGGCAAAACCAAGCTTAGAGCGAGGCCGCATTGAGGAGATTTTAGATGCAAATCTTTTGACAGAGCCATGCAACACAGAGATGATGCTAAATATGGGGAAACTTGGCCTAAGATGTGTTGTGAAAGTGCCCAAAAACCGCCCTACAATGTCGATGGTGTGGCAAGAACTCGAAGCGGCCCTCTACTGTGCGAACAATTTCATTAGCAAACATCCTTCAAGGGGATCTCGAAGATCAGCTGGTACATCCCGCCGACCATCAGATATAGGGCTTAGAAGATCGATGGACTACGACCAGTCTCAAAACTCGGTGAGCATAGATGACATTGGATTCCAGAGGTTTCATGTCGACATAAATAGCATCTCCTTACGGAGCACAAGCTTAGGGTGTTTGGAGTTGGACGATAGCAGCATCATCGTCGACATCGAAAATTTGGAAGATATACATGAGGAATCTGTTAGAGAAAAAGAATTGAACATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0009814 GO:0009817 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0030054 GO:0036211 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0046777 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0071704 GO:0071944 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

417

Amino Acids

47.0

Weight (kDa)

7.59

Isoelectric Point (pI)

49.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	64 - 311	9.4e-25	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	65 - 126	2.2e-07	Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr	PF07714	133 - 313	1.8e-17	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014635)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g11130
malus_domestica	MD02G1123900.v1.1 MD15G1237900.v1.1
prunus_persica	Prupe.7G174600_v2.0.a1 Prupe.7G174600_v2.0.a1 Prupe.7G174600_v2.0.a1
pyrus_communis	pycom02g09680
rosa_chinensis	RchiOBHm_Chr2g0098411
rosa_laevigata	RLG00000016797
rosa_multiflora	Rmu_ssc0000196.1_g000021
rosa_roxburghii	Rroxscaffold_2G00143990
rosa_rugosa	Rorug02G0073000
rosa_samantha	Rh2AG120300 Rh2BG123100 Rh2CG124700 Rh2DG124800
rosa_wichuraiana	Rw2G009410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	2 cut(s)	1116, 1188
AccII	CGCG	1 cut(s)	495
AciI	CCGC	7 cut(s)	273, 493, 745, 796, 907, 944, 1018
AclWI	GGATC	3 cut(s)	385, 591, 1000
AcoI	YGGCCR	1 cut(s)	719
AcsI	RAATTY	5 cut(s)	112, 178, 299, 530, 1198
AcyI	GRCGYC	1 cut(s)	662
AfaI	GTAC	2 cut(s)	351, 1012
AfiI	CCNNNNNNNGG	2 cut(s)	802, 989
AflIII	ACRYGT	2 cut(s)	592, 1245
AgsI	TTSAA	8 cut(s)	49, 118, 229, 536, 648, 735, 987, 1243
AhlI	ACTAGT	1 cut(s)	160
AjnI	CCWGG	1 cut(s)	619
AluBI	AGCT	5 cut(s)	402, 505, 783, 1007, 1149
AluI	AGCT	5 cut(s)	402, 505, 783, 1007, 1149
Alw21I	GWGCWC	3 cut(s)	66, 744, 1145
Alw26I	GTCTC	1 cut(s)	1071
AlwI	GGATC	3 cut(s)	385, 591, 1000
AoxI	GGCC	4 cut(s)	719, 793, 874, 945
ApeKI	GCWGC	3 cut(s)	440, 600, 1176
ApoI	RAATTY	5 cut(s)	112, 178, 299, 530, 1198
Asp700I	GAANNNNTTC	1 cut(s)	965
AspLEI	GCGC	1 cut(s)	144
AspS9I	GGNCC	3 cut(s)	234, 572, 946
AsuHPI	GGTGA	1 cut(s)	1090
AvaII	GGWCC	2 cut(s)	234, 572
BaeGI	GKGCMC	1 cut(s)	900
BarI	GAAGNNNNNNTAC	2 cut(s)	303, 335
BbsI	GAAGAC	1 cut(s)	217
Bbv12I	GWGCWC	3 cut(s)	66, 744, 1145
BbvI	GCAGC	3 cut(s)	452, 612, 1188
BccI	CCATC	3 cut(s)	916, 1033, 1045
BceAI	ACGGC	1 cut(s)	649
BcgI	CGANNNNNNTGC	4 cut(s)	1165, 1168, 1199, 1202
BciT130I	CCWGG	1 cut(s)	621
BciVI	GTATCC	1 cut(s)	617
BcoDI	GTCTC	1 cut(s)	1071
BcuI	ACTAGT	1 cut(s)	160
BfaI	CTAG	4 cut(s)	161, 257, 327, 629
BfmI	CTRYAG	2 cut(s)	55, 679
BfoI	RGCGCY	1 cut(s)	145
BfuI	GTATCC	1 cut(s)	617
BisI	GCNGC	5 cut(s)	441, 601, 796, 945, 1177
BlsI	GCNGC	5 cut(s)	442, 602, 797, 946, 1178
Bme1390I	CCNGG	1 cut(s)	621
Bme18I	GGWCC	2 cut(s)	234, 572
BmgT120I	GGNCC	3 cut(s)	234, 572, 946
BmiI	GGNNCC	3 cut(s)	205, 235, 253
BmrFI	CCNGG	1 cut(s)	621
BmrI	ACTGGG	2 cut(s)	588, 778
BmsI	GCATC	4 cut(s)	805, 843, 1137, 1188
BmuI	ACTGGG	2 cut(s)	588, 778
BpiI	GAAGAC	1 cut(s)	217
BpmI	CTGGAG	1 cut(s)	603
Bpu10I	CCTNAGC	1 cut(s)	1150
Bsa29I	ATCGAT	1 cut(s)	1049
BsaBI	GATNNNNATC	1 cut(s)	819
BsaHI	GRCGYC	1 cut(s)	662
BsaJI	CCNNGG	1 cut(s)	540
BsaWI	WCCGGW	1 cut(s)	206
Bsc4I	CCNNNNNNNGG	2 cut(s)	802, 989
Bse1I	ACTGG	5 cut(s)	148, 583, 722, 773, 1063
Bse3DI	GCAATG	1 cut(s)	450
Bse8I	GATNNNNATC	1 cut(s)	819
BseBI	CCWGG	1 cut(s)	621
BseCI	ATCGAT	1 cut(s)	1049
BseDI	CCNNGG	1 cut(s)	540
BseGI	GGATG	3 cut(s)	160, 979, 1013
BseJI	GATNNNNATC	1 cut(s)	819
BseLI	CCNNNNNNNGG	2 cut(s)	802, 989
BseMI	GCAATG	1 cut(s)	450
BseNI	ACTGG	5 cut(s)	148, 583, 722, 773, 1063
BseRI	GAGGAG	2 cut(s)	412, 818
BseSI	GKGCMC	1 cut(s)	900
BseXI	GCAGC	3 cut(s)	452, 612, 1188
Bsh1236I	CGCG	1 cut(s)	495
BshFI	GGCC	4 cut(s)	721, 795, 876, 947
BshVI	ATCGAT	1 cut(s)	1049
BsiHKAI	GWGCWC	3 cut(s)	66, 744, 1145
BsiSI	CCGG	1 cut(s)	207
BslFI	GGGAC	1 cut(s)	247
BslI	CCNNNNNNNGG	2 cut(s)	802, 989
BsmAI	GTCTC	1 cut(s)	1071
BsmFI	GGGAC	1 cut(s)	247
BsnI	GGCC	4 cut(s)	721, 795, 876, 947
Bsp1286I	GDGCHC	4 cut(s)	66, 744, 900, 1145
Bsp143I	GATC	5 cut(s)	377, 583, 992, 1001, 1046
BspACI	CCGC	7 cut(s)	273, 493, 745, 796, 907, 944, 1018
BspANI	GGCC	4 cut(s)	721, 795, 876, 947
BspDI	ATCGAT	1 cut(s)	1049
BspFNI	CGCG	1 cut(s)	495
BspLI	GGNNCC	3 cut(s)	205, 235, 253
BspMAI	CTGCAG	1 cut(s)	59
BspPI	GGATC	3 cut(s)	385, 591, 1000
BsrDI	GCAATG	1 cut(s)	450
BsrI	ACTGG	5 cut(s)	148, 583, 722, 773, 1063
BssECI	CCNNGG	1 cut(s)	540
BssMI	GATC	5 cut(s)	377, 583, 992, 1001, 1046
BssNI	GRCGYC	1 cut(s)	662
BssT1I	CCWWGG	1 cut(s)	540
Bst2UI	CCWGG	1 cut(s)	621
Bst4CI	ACNGT	1 cut(s)	956
Bst6I	CTCTTC	1 cut(s)	117
BstACI	GRCGYC	1 cut(s)	662
BstAPI	GCANNNNNTGC	1 cut(s)	192
BstDEI	CTNAG	5 cut(s)	174, 784, 878, 1040, 1150
BstF5I	GGATG	3 cut(s)	160, 979, 1013
BstFNI	CGCG	1 cut(s)	495
BstH2I	RGCGCY	1 cut(s)	145
BstHHI	GCGC	1 cut(s)	144
BstKTI	GATC	5 cut(s)	380, 586, 995, 1004, 1049
BstMAI	GTCTC	1 cut(s)	1071
BstMBI	GATC	5 cut(s)	377, 583, 992, 1001, 1046
BstMWI	GCNNNNNNNGC	2 cut(s)	192, 437
BstNI	CCWGG	1 cut(s)	621
BstNSI	RCATGY	2 cut(s)	596, 1249
BstSCI	CCNGG	1 cut(s)	619
BstSFI	CTRYAG	2 cut(s)	55, 679
BstSLI	GKGCMC	1 cut(s)	900
BstUI	CGCG	1 cut(s)	495
BstV1I	GCAGC	3 cut(s)	452, 612, 1188
BstV2I	GAAGAC	1 cut(s)	217
BstX2I	RGATCY	1 cut(s)	992
BstYI	RGATCY	1 cut(s)	992
Bsu15I	ATCGAT	1 cut(s)	1049
BsuI	GTATCC	1 cut(s)	617
BsuRI	GGCC	4 cut(s)	721, 795, 876, 947
BsuTUI	ATCGAT	1 cut(s)	1049
BtgZI	GCGATG	1 cut(s)	687
BtsCI	GGATG	3 cut(s)	160, 979, 1013
BtsI	GCAGTG	1 cut(s)	193
BtsIMutI	CAGTG	2 cut(s)	193, 729
CfoI	GCGC	1 cut(s)	144
Cfr13I	GGNCC	3 cut(s)	234, 572, 946
ClaI	ATCGAT	1 cut(s)	1049
CseI	GACGC	1 cut(s)	670
Csp6I	GTAC	2 cut(s)	350, 1011
CviAII	CATG	7 cut(s)	464, 593, 635, 837, 1112, 1214, 1246
CviQI	GTAC	2 cut(s)	350, 1011
DdeI	CTNAG	5 cut(s)	174, 784, 878, 1040, 1150
DpnI	GATC	5 cut(s)	379, 585, 994, 1003, 1048
DpnII	GATC	5 cut(s)	377, 583, 992, 1001, 1046
EaeI	YGGCCR	1 cut(s)	719
Eam1104I	CTCTTC	1 cut(s)	117
EarI	CTCTTC	1 cut(s)	117
Eco130I	CCWWGG	1 cut(s)	540
Eco47I	GGWCC	2 cut(s)	234, 572
EcoO109I	RGGNCCY	2 cut(s)	234, 572
EcoRI	GAATTC	1 cut(s)	299
EcoRII	CCWGG	1 cut(s)	619
EcoT14I	CCWWGG	1 cut(s)	540
ErhI	CCWWGG	1 cut(s)	540
FaeI	CATG	7 cut(s)	467, 596, 638, 840, 1115, 1217, 1249
FalI	AAGNNNNNCTT	2 cut(s)	221, 253
FaqI	GGGAC	1 cut(s)	247
FatI	CATG	7 cut(s)	463, 592, 634, 836, 1111, 1213, 1245
FauI	CCCGC	1 cut(s)	1025
FauNDI	CATATG	1 cut(s)	92
FblI	GTMKAC	2 cut(s)	1116, 1188
Fnu4HI	GCNGC	5 cut(s)	441, 601, 796, 945, 1177
FokI	GGATG	3 cut(s)	167, 966, 1000
Fsp4HI	GCNGC	5 cut(s)	441, 601, 796, 945, 1177
FspBI	CTAG	4 cut(s)	161, 257, 327, 629
GlaI	GCGC	1 cut(s)	143
GluI	GCNGC	5 cut(s)	441, 601, 796, 945, 1177
GsuI	CTGGAG	1 cut(s)	603
HaeII	RGCGCY	1 cut(s)	145
HaeIII	GGCC	4 cut(s)	721, 795, 876, 947
HapII	CCGG	1 cut(s)	207
HgaI	GACGC	1 cut(s)	670
HhaI	GCGC	1 cut(s)	144
Hin1I	GRCGYC	1 cut(s)	662
Hin1II	CATG	7 cut(s)	467, 596, 638, 840, 1115, 1217, 1249
Hin6I	GCGC	1 cut(s)	142
HinP1I	GCGC	1 cut(s)	142
HincII	GTYRAC	3 cut(s)	424, 1117, 1189
HindII	GTYRAC	3 cut(s)	424, 1117, 1189
HindIII	AAGCTT	3 cut(s)	503, 781, 1147
HinfI	GANTC	4 cut(s)	420, 731, 1098, 1220
HpaII	CCGG	1 cut(s)	207
HphI	GGTGA	1 cut(s)	1090
Hpy166II	GTNNAC	4 cut(s)	424, 475, 1117, 1189
Hpy188I	TCNGA	3 cut(s)	305, 550, 1030
Hpy188III	TCNNGA	5 cut(s)	384, 517, 570, 996, 1102
Hpy8I	GTNNAC	4 cut(s)	424, 475, 1117, 1189
Hpy99I	CGWCG	1 cut(s)	1190
HpyAV	CCTTC	4 cut(s)	160, 368, 461, 993
HpyCH4III	ACNGT	1 cut(s)	956
HpyCH4IV	ACGT	1 cut(s)	352
HpyCH4V	TGCA	5 cut(s)	57, 218, 703, 818, 840
HpyF10VI	GCNNNNNNNGC	2 cut(s)	192, 437
HpyF3I	CTNAG	5 cut(s)	174, 784, 878, 1040, 1150
HpySE526I	ACGT	1 cut(s)	352
Hsp92I	GRCGYC	1 cut(s)	662
Hsp92II	CATG	7 cut(s)	467, 596, 638, 840, 1115, 1217, 1249
HspAI	GCGC	1 cut(s)	142
Kzo9I	GATC	5 cut(s)	377, 583, 992, 1001, 1046
LmnI	GCTCC	3 cut(s)	399, 437, 1140
Lsp1109I	GCAGC	3 cut(s)	452, 612, 1188
LweI	GCATC	4 cut(s)	805, 843, 1137, 1188
MaeI	CTAG	4 cut(s)	161, 257, 327, 629
MaeII	ACGT	1 cut(s)	352
MaeIII	GTNAC	3 cut(s)	37, 83, 318
MalI	GATC	5 cut(s)	379, 585, 994, 1003, 1048
MboI	GATC	5 cut(s)	377, 583, 992, 1001, 1046
MflI	RGATCY	1 cut(s)	992
MhlI	GDGCHC	4 cut(s)	66, 744, 900, 1145
MluCI	AATT	8 cut(s)	112, 178, 277, 299, 530, 964, 1198, 1238
MlyI	GAGTC	1 cut(s)	429
MmeI	TCCRAC	3 cut(s)	132, 340, 1146
MnlI	CCTC	9 cut(s)	132, 309, 390, 405, 785, 796, 959, 1098, 1210
MroXI	GAANNNNTTC	1 cut(s)	965
MseI	TTAA	4 cut(s)	12, 264, 404, 507
MslI	CAYNNNNRTG	2 cut(s)	462, 848
MspA1I	CMGCKG	1 cut(s)	1007
MspI	CCGG	1 cut(s)	207
MspR9I	CCNGG	1 cut(s)	621
MvaI	CCWGG	1 cut(s)	621
MvnI	CGCG	1 cut(s)	495
MwoI	GCNNNNNNNGC	2 cut(s)	192, 437
NdeI	CATATG	1 cut(s)	92
NdeII	GATC	5 cut(s)	377, 583, 992, 1001, 1046
NlaIII	CATG	7 cut(s)	467, 596, 638, 840, 1115, 1217, 1249
NlaIV	GGNNCC	3 cut(s)	205, 235, 253
NmeAIII	GCCGAG	1 cut(s)	697
NmuCI	GTSAC	1 cut(s)	83
NspI	RCATGY	2 cut(s)	596, 1249
PciI	ACATGT	2 cut(s)	592, 1245
PcsI	WCGNNNNNNNCGW	1 cut(s)	1191
PdmI	GAANNNNTTC	1 cut(s)	965
PfeI	GAWTC	3 cut(s)	731, 1098, 1220
PfoI	TCCNGGA	1 cut(s)	619
PkrI	GCNGC	5 cut(s)	442, 602, 797, 946, 1178
PleI	GAGTC	1 cut(s)	428
PpsI	GAGTC	1 cut(s)	428
PpuMI	RGGWCCY	2 cut(s)	234, 572
PscI	ACATGT	2 cut(s)	592, 1245
Psp5II	RGGWCCY	2 cut(s)	234, 572
Psp6I	CCWGG	1 cut(s)	619
PspGI	CCWGG	1 cut(s)	619
PspN4I	GGNNCC	3 cut(s)	205, 235, 253
PspPI	GGNCC	3 cut(s)	234, 572, 946
PspPPI	RGGWCCY	2 cut(s)	234, 572
PstI	CTGCAG	1 cut(s)	59
PsuI	RGATCY	1 cut(s)	992
PvuII	CAGCTG	1 cut(s)	1007
RsaI	GTAC	2 cut(s)	351, 1012
RsaNI	GTAC	2 cut(s)	350, 1011
RseI	CAYNNNNRTG	2 cut(s)	462, 848
SalI	GTCGAC	2 cut(s)	1115, 1187
SaqAI	TTAA	4 cut(s)	12, 264, 404, 507
SatI	GCNGC	5 cut(s)	441, 601, 796, 945, 1177
Sau3AI	GATC	5 cut(s)	377, 583, 992, 1001, 1046
Sau96I	GGNCC	3 cut(s)	234, 572, 946
SchI	GAGTC	1 cut(s)	429
ScrFI	CCNGG	1 cut(s)	621
SduI	GDGCHC	4 cut(s)	66, 744, 900, 1145
SfaNI	GCATC	4 cut(s)	805, 843, 1137, 1188
SfcI	CTRYAG	2 cut(s)	55, 679
SinI	GGWCC	2 cut(s)	234, 572
SmiMI	CAYNNNNRTG	2 cut(s)	462, 848
SpeI	ACTAGT	1 cut(s)	160
Sse9I	AATT	8 cut(s)	112, 178, 277, 299, 530, 964, 1198, 1238
SsiI	CCGC	7 cut(s)	273, 493, 745, 796, 907, 944, 1018
SspMI	CTAG	4 cut(s)	161, 257, 327, 629
StyD4I	CCNGG	1 cut(s)	619
StyI	CCWWGG	1 cut(s)	540
TaaI	ACNGT	1 cut(s)	956
TaiI	ACGT	1 cut(s)	355
TaqI	TCGA	9 cut(s)	68, 294, 920, 939, 997, 1049, 1116, 1188, 1194
TasI	AATT	8 cut(s)	112, 178, 277, 299, 530, 964, 1198, 1238
TauI	GCSGC	2 cut(s)	798, 947
TfiI	GAWTC	3 cut(s)	731, 1098, 1220
Tru1I	TTAA	4 cut(s)	12, 264, 404, 507
Tru9I	TTAA	4 cut(s)	12, 264, 404, 507
TscAI	CASTG	2 cut(s)	193, 729
TseFI	GTSAC	1 cut(s)	83
TseI	GCWGC	3 cut(s)	440, 600, 1176
Tsp45I	GTSAC	1 cut(s)	83
TspDTI	ATGAA	8 cut(s)	171, 270, 275, 324, 480, 543, 958, 1100
TspGWI	ACGGA	2 cut(s)	6, 1153
TspRI	CASTG	2 cut(s)	193, 729
VpaK11BI	GGWCC	2 cut(s)	234, 572
XapI	RAATTY	5 cut(s)	112, 178, 299, 530, 1198
XceI	RCATGY	2 cut(s)	596, 1249
XmiI	GTMKAC	2 cut(s)	1116, 1188
XmnI	GAANNNNTTC	1 cut(s)	965
XspI	CTAG	4 cut(s)	161, 257, 327, 629

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.