Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0014635

Physical Location & Seq

Genomic Coordinates Forward (+)

10441189 .. 10443460

Gene Structure 2272 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG123100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1404 bp

ATGGATTCACCACAGTCTCTTCAAGATTCTGTTCCTCATGCTAGTCATCAAACACATATATGGGGAGGCTTCGTTATAGGCATTCTAACAGTCGTGATAGCTGCGTTGTTATACTTCCTTTGCAGCAGGAAGCTTATACCGGCTCTCAGGCGCCGGCGAGAACAAGGGCAGTTGTCACCAAAGAAACTTGCTCTAAAAAGAACTTTGAAACAGGAGAAGATAGATCTGAGGAGATTCCATCTGGAGGAACTAGTGAAGGCTACAAATAATTTCAACAGAGACTGCATGTTGGGCTGTGGTGCTTTTGCAAATGTTTACAAGGGGAACTTTGATGATCTGGGAACCCTGGCCATCAAGAGAGCTCACGCCGATTCGTTTCAGAGTGTTGAAGAATTCAGAAACGAAGTGAGGCTACTCTCTAAAGTTAAGCACAGAAACCTTGTAGGACTGGTTGGATATTGTGAAGAACCTGTTGGAGCAAGAGGGGGAAAAGTTTTGATCTATGAATACGTACCCAATGGTTCTCTGCTTGATTATATAATGGGAAAGAAATGGATAAGCTTAAGTTGGAGGCAAAGAGTGAACATAGCCATTGGAGCAGCAAAAGGCATTGCTCATTTGCATGAAGGGGTTAACCCAAGCATAATTCACCGTGACATAAAGCCAAGTAACATCTTGATAGGAGATGGGTTTGAAGCCAAGGTTTCAGATTTTGGACTTGTAAAATCAGGGCCTGTAGGAGACGATTCACATGTCAGTAGCCAAATCAAAGGAACTCCAGGGTACCTCGACCCTGCTTATTGTGCAAGTTTCCACTTATCCCAATTCAGTGATGTCTATAGCTTTGGAATCATTCTTCTCCAACTTGTTTCTTCCCGGCCTGCTGTTGATATGACCGGAAATCGCCCTAATTTTCACATCATTGACTGGGCAAGGCCTAGCTTAGAGAGAGGTCGTGTTGAGGAGATTTTAGATGCTAATCTTTTATCAGAACCATGTAACACAGAGGTGATGCTAAAGATGGGAAAACTTGGCTTGAGGTGTGTTGTGAAAATACCCAAGAACCGTCCTACAATGTCCCAGGTGTGTCGAGAACTCGAAGAAGCCCTCTATTCTGCAGACAATAAACAGCAGTTGAGAGAGAGTCGAAGATCAAATGGTGCACCTCGTCAACCAACAGACCTAGGGTTGGGGAGATCAATAGACTATGATCAATCTCTAAACTCTGTGAGCCTAGATGGCATTGGATTCCAGAAGTTTCATGTAGACCTAGATAGCATGTCCTTTCAGAGCACAAGCTTAAGGTGTTTAGAGAACAACAGTATTGAAATCGACATCGACGGGGACGATTTGAAAGATATACGAGAGGAAATTAGTACAGAAGAAGACCCAAACATGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0009814 GO:0009817 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0030054 GO:0036211 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0046777 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0071704 GO:0071944 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

467

Amino Acids

52.2

Weight (kDa)

7.62

Isoelectric Point (pI)

52.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	95 - 366	1.1e-34	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	95 - 364	2.1e-34	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014635)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g11130
malus_domestica	MD02G1123900.v1.1 MD15G1237900.v1.1
prunus_persica	Prupe.7G174600_v2.0.a1 Prupe.7G174600_v2.0.a1 Prupe.7G174600_v2.0.a1
pyrus_communis	pycom02g09680
rosa_chinensis	RchiOBHm_Chr2g0098411
rosa_laevigata	RLG00000016797
rosa_multiflora	Rmu_ssc0000196.1_g000021
rosa_roxburghii	Rroxscaffold_2G00143990
rosa_rugosa	Rorug02G0073000
rosa_samantha	Rh2AG120300 Rh2BG123100 Rh2CG124700 Rh2DG124800
rosa_wichuraiana	Rw2G009410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	783
AccB1I	GGYRCC	2 cut(s)	150, 783
AccI	GTMKAC	1 cut(s)	1266
AcoI	YGGCCR	1 cut(s)	348
AcsI	RAATTY	1 cut(s)	392
AcyI	GRCGYC	1 cut(s)	151
AfaI	GTAC	3 cut(s)	513, 785, 1378
AfiI	CCNNNNNNNGG	2 cut(s)	244, 1189
AflII	CTTAAG	2 cut(s)	562, 1300
AflIII	ACRYGT	1 cut(s)	751
AgsI	TTSAA	7 cut(s)	23, 208, 274, 389, 695, 1328, 1354
AhlI	ACTAGT	1 cut(s)	250
AjnI	CCWGG	3 cut(s)	345, 778, 1080
AjuI	GAANNNNNNNTTGG	2 cut(s)	456, 488
AleI	CACNNNNGTG	1 cut(s)	1007
AluBI	AGCT	7 cut(s)	101, 133, 362, 561, 843, 942, 1299
AluI	AGCT	7 cut(s)	101, 133, 362, 561, 843, 942, 1299
Alw21I	GWGCWC	3 cut(s)	364, 1165, 1295
Alw26I	GTCTC	3 cut(s)	21, 273, 735
Alw44I	GTGCAC	1 cut(s)	1161
AlwNI	CAGNNNCTG	2 cut(s)	282, 734
AoxI	GGCC	4 cut(s)	348, 731, 878, 935
ApaLI	GTGCAC	1 cut(s)	1161
ApeKI	GCWGC	3 cut(s)	101, 123, 599
ApoI	RAATTY	1 cut(s)	392
Asp718I	GGTACC	1 cut(s)	783
AspA2I	CCTAGG	1 cut(s)	1183
AspLEI	GCGC	1 cut(s)	153
AspS9I	GGNCC	1 cut(s)	731
AsuC2I	CCSGG	1 cut(s)	877
AsuHPI	GGTGA	3 cut(s)	168, 641, 1021
AvrII	CCTAGG	1 cut(s)	1183
BaeGI	GKGCMC	1 cut(s)	1165
BalI	TGGCCA	1 cut(s)	350
BanI	GGYRCC	2 cut(s)	150, 783
BanII	GRGCYC	1 cut(s)	364
BarI	GAAGNNNNNNTAC	2 cut(s)	396, 428
BbsI	GAAGAC	1 cut(s)	1392
Bbv12I	GWGCWC	3 cut(s)	364, 1165, 1295
BbvI	GCAGC	3 cut(s)	88, 135, 611
BccI	CCATC	5 cut(s)	246, 359, 680, 1015, 1232
BciT130I	CCWGG	3 cut(s)	347, 780, 1082
BclI	TGATCA	1 cut(s)	1210
BcnI	CCSGG	1 cut(s)	877
BcoDI	GTCTC	3 cut(s)	21, 273, 735
BcuI	ACTAGT	1 cut(s)	250
BfaI	CTAG	6 cut(s)	42, 251, 939, 1184, 1235, 1271
BfmI	CTRYAG	3 cut(s)	735, 838, 1116
BfoI	RGCGCY	1 cut(s)	154
BfrI	CTTAAG	2 cut(s)	562, 1300
BglI	GCCNNNNNGGC	1 cut(s)	1239
BglII	AGATCT	1 cut(s)	223
BisI	GCNGC	3 cut(s)	102, 124, 600
BlnI	CCTAGG	1 cut(s)	1183
BlsI	GCNGC	3 cut(s)	103, 125, 601
Bme1390I	CCNGG	4 cut(s)	347, 780, 877, 1082
BmgT120I	GGNCC	1 cut(s)	731
BmiI	GGNNCC	3 cut(s)	152, 343, 785
BmrFI	CCNGG	4 cut(s)	347, 780, 877, 1082
BmrI	ACTGGG	1 cut(s)	937
BmsI	GCATC	2 cut(s)	964, 1002
BmuI	ACTGGG	1 cut(s)	937
BpiI	GAAGAC	1 cut(s)	1392
BpmI	CTGGAG	2 cut(s)	263, 762
BpuEI	CTTGAG	1 cut(s)	1057
BpuMI	CCSGG	1 cut(s)	877
BsaAI	YACGTR	1 cut(s)	511
BsaBI	GATNNNNATC	1 cut(s)	978
BsaHI	GRCGYC	1 cut(s)	151
BsaJI	CCNNGG	5 cut(s)	345, 699, 779, 1080, 1183
BsaWI	WCCGGW	1 cut(s)	896
BsaXI	ACNNNNNCTCC	2 cut(s)	57, 87
Bsc4I	CCNNNNNNNGG	2 cut(s)	244, 1189
Bse118I	RCCGGY	2 cut(s)	139, 153
Bse1I	ACTGG	2 cut(s)	453, 932
Bse3DI	GCAATG	1 cut(s)	609
Bse8I	GATNNNNATC	1 cut(s)	978
BseBI	CCWGG	3 cut(s)	347, 780, 1082
BseDI	CCNNGG	5 cut(s)	345, 699, 779, 1080, 1183
BseJI	GATNNNNATC	1 cut(s)	978
BseLI	CCNNNNNNNGG	2 cut(s)	244, 1189
BseMI	GCAATG	1 cut(s)	609
BseMII	CTCAG	2 cut(s)	160, 218
BseNI	ACTGG	2 cut(s)	453, 932
BseRI	GAGGAG	2 cut(s)	244, 977
BseSI	GKGCMC	1 cut(s)	1165
BseXI	GCAGC	3 cut(s)	88, 135, 611
BshFI	GGCC	4 cut(s)	350, 733, 880, 937
BshNI	GGYRCC	2 cut(s)	150, 783
BsiHKAI	GWGCWC	3 cut(s)	364, 1165, 1295
BsiSI	CCGG	4 cut(s)	140, 154, 877, 897
BslFI	GGGAC	2 cut(s)	1063, 1358
BslI	CCNNNNNNNGG	2 cut(s)	244, 1189
BsmAI	GTCTC	3 cut(s)	21, 273, 735
BsmBI	CGTCTC	1 cut(s)	735
BsmFI	GGGAC	2 cut(s)	1063, 1358
BsmI	GAATGC	1 cut(s)	81
BsnI	GGCC	4 cut(s)	350, 733, 880, 937
Bsp1286I	GDGCHC	3 cut(s)	364, 1165, 1295
Bsp143I	GATC	6 cut(s)	223, 334, 498, 1151, 1196, 1210
BspANI	GGCC	4 cut(s)	350, 733, 880, 937
BspCNI	CTCAG	2 cut(s)	159, 219
BspLI	GGNNCC	3 cut(s)	152, 343, 785
BspMAI	CTGCAG	1 cut(s)	1120
BspT107I	GGYRCC	2 cut(s)	150, 783
BspTI	CTTAAG	2 cut(s)	562, 1300
BsrDI	GCAATG	1 cut(s)	609
BsrFI	RCCGGY	2 cut(s)	139, 153
BsrI	ACTGG	2 cut(s)	453, 932
BssAI	RCCGGY	2 cut(s)	139, 153
BssECI	CCNNGG	5 cut(s)	345, 699, 779, 1080, 1183
BssMI	GATC	6 cut(s)	223, 334, 498, 1151, 1196, 1210
BssNI	GRCGYC	1 cut(s)	151
BssT1I	CCWWGG	2 cut(s)	699, 1183
Bst2UI	CCWGG	3 cut(s)	347, 780, 1082
Bst4CI	ACNGT	5 cut(s)	15, 91, 653, 1067, 1322
Bst6I	CTCTTC	1 cut(s)	24
BstACI	GRCGYC	1 cut(s)	151
BstAFI	CTTAAG	2 cut(s)	562, 1300
BstBAI	YACGTR	1 cut(s)	511
BstC8I	GCNNGC	2 cut(s)	155, 882
BstDEI	CTNAG	3 cut(s)	146, 227, 943
BstH2I	RGCGCY	1 cut(s)	154
BstHHI	GCGC	1 cut(s)	153
BstKTI	GATC	6 cut(s)	226, 337, 501, 1154, 1199, 1213
BstMAI	GTCTC	3 cut(s)	21, 273, 735
BstMBI	GATC	6 cut(s)	223, 334, 498, 1151, 1196, 1210
BstMWI	GCNNNNNNNGC	4 cut(s)	291, 596, 803, 1239
BstNI	CCWGG	3 cut(s)	347, 780, 1082
BstNSI	RCATGY	3 cut(s)	289, 755, 1282
BstSCI	CCNGG	4 cut(s)	345, 778, 875, 1080
BstSFI	CTRYAG	3 cut(s)	735, 838, 1116
BstSLI	GKGCMC	1 cut(s)	1165
BstSNI	TACGTA	1 cut(s)	511
BstV1I	GCAGC	3 cut(s)	88, 135, 611
BstV2I	GAAGAC	1 cut(s)	1392
BstX2I	RGATCY	1 cut(s)	223
BstYI	RGATCY	1 cut(s)	223
BsuRI	GGCC	4 cut(s)	350, 733, 880, 937
BtsIMutI	CAGTG	1 cut(s)	835
Cac8I	GCNNGC	2 cut(s)	155, 882
CaiI	CAGNNNCTG	2 cut(s)	282, 734
CfoI	GCGC	1 cut(s)	153
Cfr10I	RCCGGY	2 cut(s)	139, 153
Cfr13I	GGNCC	1 cut(s)	731
Csp6I	GTAC	3 cut(s)	512, 784, 1377
CviAII	CATG	8 cut(s)	38, 286, 623, 752, 996, 1262, 1279, 1396
CviQI	GTAC	3 cut(s)	512, 784, 1377
DdeI	CTNAG	3 cut(s)	146, 227, 943
DinI	GGCGCC	1 cut(s)	152
DpnI	GATC	6 cut(s)	225, 336, 500, 1153, 1198, 1212
DpnII	GATC	6 cut(s)	223, 334, 498, 1151, 1196, 1210
EaeI	YGGCCR	1 cut(s)	348
Eam1104I	CTCTTC	1 cut(s)	24
EarI	CTCTTC	1 cut(s)	24
Ecl136II	GAGCTC	1 cut(s)	362
Eco105I	TACGTA	1 cut(s)	511
Eco130I	CCWWGG	2 cut(s)	699, 1183
Eco147I	AGGCCT	1 cut(s)	937
Eco24I	GRGCYC	1 cut(s)	364
Eco53kI	GAGCTC	1 cut(s)	362
EcoICRI	GAGCTC	1 cut(s)	362
EcoO109I	RGGNCCY	1 cut(s)	731
EcoRI	GAATTC	1 cut(s)	392
EcoRII	CCWGG	3 cut(s)	345, 778, 1080
EcoT14I	CCWWGG	2 cut(s)	699, 1183
EcoT38I	GRGCYC	1 cut(s)	364
EgeI	GGCGCC	1 cut(s)	152
EheI	GGCGCC	1 cut(s)	152
ErhI	CCWWGG	2 cut(s)	699, 1183
Esp3I	CGTCTC	1 cut(s)	735
FaeI	CATG	8 cut(s)	41, 289, 626, 755, 999, 1265, 1282, 1399
FalI	AAGNNNNNCTT	2 cut(s)	311, 343
FaqI	GGGAC	2 cut(s)	1063, 1358
FatI	CATG	8 cut(s)	37, 285, 622, 751, 995, 1261, 1278, 1395
FbaI	TGATCA	1 cut(s)	1210
FblI	GTMKAC	1 cut(s)	1266
Fnu4HI	GCNGC	3 cut(s)	102, 124, 600
FriOI	GRGCYC	1 cut(s)	364
Fsp4HI	GCNGC	3 cut(s)	102, 124, 600
FspBI	CTAG	6 cut(s)	42, 251, 939, 1184, 1235, 1271
GlaI	GCGC	1 cut(s)	152
GluI	GCNGC	3 cut(s)	102, 124, 600
GsuI	CTGGAG	2 cut(s)	263, 762
HaeII	RGCGCY	1 cut(s)	154
HaeIII	GGCC	4 cut(s)	350, 733, 880, 937
HapII	CCGG	4 cut(s)	140, 154, 877, 897
HhaI	GCGC	1 cut(s)	153
Hin1I	GRCGYC	1 cut(s)	151
Hin1II	CATG	8 cut(s)	41, 289, 626, 755, 999, 1265, 1282, 1399
Hin6I	GCGC	1 cut(s)	151
HinP1I	GCGC	1 cut(s)	151
HincII	GTYRAC	2 cut(s)	634, 1172
HindII	GTYRAC	2 cut(s)	634, 1172
HindIII	AAGCTT	3 cut(s)	131, 559, 1297
HinfI	GANTC	8 cut(s)	5, 26, 234, 371, 746, 849, 1144, 1248
HpaI	GTTAAC	1 cut(s)	634
HpaII	CCGG	4 cut(s)	140, 154, 877, 897
HphI	GGTGA	3 cut(s)	168, 641, 1021
Hpy166II	GTNNAC	6 cut(s)	316, 583, 634, 1163, 1172, 1267
Hpy188I	TCNGA	6 cut(s)	228, 381, 398, 709, 991, 1290
Hpy188III	TCNNGA	7 cut(s)	23, 94, 242, 355, 676, 1091, 1252
Hpy8I	GTNNAC	6 cut(s)	316, 583, 634, 1163, 1172, 1267
Hpy99I	CGWCG	1 cut(s)	1343
HpyAV	CCTTC	2 cut(s)	250, 620
HpyCH4III	ACNGT	5 cut(s)	15, 91, 653, 1067, 1322
HpyCH4IV	ACGT	1 cut(s)	510
HpyCH4V	TGCA	7 cut(s)	123, 285, 308, 622, 806, 1118, 1163
HpyF10VI	GCNNNNNNNGC	4 cut(s)	291, 596, 803, 1239
HpyF3I	CTNAG	3 cut(s)	146, 227, 943
HpySE526I	ACGT	1 cut(s)	510
Hsp92I	GRCGYC	1 cut(s)	151
Hsp92II	CATG	8 cut(s)	41, 289, 626, 755, 999, 1265, 1282, 1399
HspAI	GCGC	1 cut(s)	151
KasI	GGCGCC	1 cut(s)	150
KpnI	GGTACC	1 cut(s)	787
KroI	GCCGGC	1 cut(s)	153
KroNI	GCCGGC	1 cut(s)	155
Ksp22I	TGATCA	1 cut(s)	1210
KspAI	GTTAAC	1 cut(s)	634
Kzo9I	GATC	6 cut(s)	223, 334, 498, 1151, 1196, 1210
LmnI	GCTCC	2 cut(s)	476, 596
Lsp1109I	GCAGC	3 cut(s)	88, 135, 611
LweI	GCATC	2 cut(s)	964, 1002
MaeI	CTAG	6 cut(s)	42, 251, 939, 1184, 1235, 1271
MaeII	ACGT	1 cut(s)	510
MaeIII	GTNAC	4 cut(s)	174, 653, 668, 998
MalI	GATC	6 cut(s)	225, 336, 500, 1153, 1198, 1212
MboI	GATC	6 cut(s)	223, 334, 498, 1151, 1196, 1210
MflI	RGATCY	1 cut(s)	223
MhlI	GDGCHC	3 cut(s)	364, 1165, 1295
MlsI	TGGCCA	1 cut(s)	350
MluCI	AATT	6 cut(s)	268, 392, 645, 824, 910, 1371
MluNI	TGGCCA	1 cut(s)	350
Mly113I	GGCGCC	1 cut(s)	151
MlyI	GAGTC	1 cut(s)	1153
MmeI	TCCRAC	4 cut(s)	433, 454, 548, 886
Mox20I	TGGCCA	1 cut(s)	350
MreI	CGCCGGCG	1 cut(s)	153
MroNI	GCCGGC	1 cut(s)	153
MscI	TGGCCA	1 cut(s)	350
MseI	TTAA	4 cut(s)	426, 563, 633, 1301
MslI	CAYNNNNRTG	3 cut(s)	58, 621, 1007
Msp20I	TGGCCA	1 cut(s)	350
MspCI	CTTAAG	2 cut(s)	562, 1300
MspI	CCGG	4 cut(s)	140, 154, 877, 897
MspR9I	CCNGG	4 cut(s)	347, 780, 877, 1082
Mva1269I	GAATGC	1 cut(s)	81
MvaI	CCWGG	3 cut(s)	347, 780, 1082
MwoI	GCNNNNNNNGC	4 cut(s)	291, 596, 803, 1239
NaeI	GCCGGC	1 cut(s)	155
NarI	GGCGCC	1 cut(s)	151
NciI	CCSGG	1 cut(s)	877
NdeII	GATC	6 cut(s)	223, 334, 498, 1151, 1196, 1210
NgoMIV	GCCGGC	1 cut(s)	153
NlaIII	CATG	8 cut(s)	41, 289, 626, 755, 999, 1265, 1282, 1399
NlaIV	GGNNCC	3 cut(s)	152, 343, 785
NmuCI	GTSAC	2 cut(s)	174, 653
NspI	RCATGY	3 cut(s)	289, 755, 1282
OliI	CACNNNNGTG	1 cut(s)	1007
PceI	AGGCCT	1 cut(s)	937
PciI	ACATGT	1 cut(s)	751
PcsI	WCGNNNNNNNCGW	1 cut(s)	1344
PctI	GAATGC	1 cut(s)	81
PdiI	GCCGGC	1 cut(s)	155
PfeI	GAWTC	7 cut(s)	5, 26, 234, 371, 746, 849, 1248
PkrI	GCNGC	3 cut(s)	103, 125, 601
PleI	GAGTC	1 cut(s)	1152
PluTI	GGCGCC	1 cut(s)	154
PpsI	GAGTC	1 cut(s)	1152
Ppu21I	YACGTR	1 cut(s)	511
PscI	ACATGT	1 cut(s)	751
Psp124BI	GAGCTC	1 cut(s)	364
Psp6I	CCWGG	3 cut(s)	345, 778, 1080
PspGI	CCWGG	3 cut(s)	345, 778, 1080
PspN4I	GGNNCC	3 cut(s)	152, 343, 785
PspPI	GGNCC	1 cut(s)	731
PstI	CTGCAG	1 cut(s)	1120
PstNI	CAGNNNCTG	2 cut(s)	282, 734
PsuI	RGATCY	1 cut(s)	223
RsaI	GTAC	3 cut(s)	513, 785, 1378
RsaNI	GTAC	3 cut(s)	512, 784, 1377
RseI	CAYNNNNRTG	3 cut(s)	58, 621, 1007
SacI	GAGCTC	1 cut(s)	364
SaqAI	TTAA	4 cut(s)	426, 563, 633, 1301
SatI	GCNGC	3 cut(s)	102, 124, 600
Sau3AI	GATC	6 cut(s)	223, 334, 498, 1151, 1196, 1210
Sau96I	GGNCC	1 cut(s)	731
SchI	GAGTC	1 cut(s)	1153
ScrFI	CCNGG	4 cut(s)	347, 780, 877, 1082
SduI	GDGCHC	3 cut(s)	364, 1165, 1295
SfaNI	GCATC	2 cut(s)	964, 1002
SfcI	CTRYAG	3 cut(s)	735, 838, 1116
SfoI	GGCGCC	1 cut(s)	152
SgrAI	CRCCGGYG	1 cut(s)	153
SmiMI	CAYNNNNRTG	3 cut(s)	58, 621, 1007
SmlI	CTYRAG	3 cut(s)	562, 1036, 1300
SmoI	CTYRAG	3 cut(s)	562, 1036, 1300
SnaBI	TACGTA	1 cut(s)	511
SpeI	ACTAGT	1 cut(s)	250
Sse9I	AATT	6 cut(s)	268, 392, 645, 824, 910, 1371
SseBI	AGGCCT	1 cut(s)	937
SspDI	GGCGCC	1 cut(s)	150
SspMI	CTAG	6 cut(s)	42, 251, 939, 1184, 1235, 1271
SstI	GAGCTC	1 cut(s)	364
StuI	AGGCCT	1 cut(s)	937
StyD4I	CCNGG	4 cut(s)	345, 778, 875, 1080
StyI	CCWWGG	2 cut(s)	699, 1183
TaaI	ACNGT	5 cut(s)	15, 91, 653, 1067, 1322
TaiI	ACGT	1 cut(s)	513
TaqI	TCGA	6 cut(s)	789, 1090, 1098, 1147, 1332, 1338
TasI	AATT	6 cut(s)	268, 392, 645, 824, 910, 1371
TatI	WGTACW	1 cut(s)	1376
TfiI	GAWTC	7 cut(s)	5, 26, 234, 371, 746, 849, 1248
Tru1I	TTAA	4 cut(s)	426, 563, 633, 1301
Tru9I	TTAA	4 cut(s)	426, 563, 633, 1301
TscAI	CASTG	1 cut(s)	835
TseFI	GTSAC	2 cut(s)	174, 653
TseI	GCWGC	3 cut(s)	101, 123, 599
Tsp45I	GTSAC	2 cut(s)	174, 653
TspDTI	ATGAA	3 cut(s)	519, 639, 1250
TspRI	CASTG	1 cut(s)	835
Vha464I	CTTAAG	2 cut(s)	562, 1300
VneI	GTGCAC	1 cut(s)	1161
XapI	RAATTY	1 cut(s)	392
XceI	RCATGY	3 cut(s)	289, 755, 1282
XmaJI	CCTAGG	1 cut(s)	1183
XmiI	GTMKAC	1 cut(s)	1266
XspI	CTAG	6 cut(s)	42, 251, 939, 1184, 1235, 1271

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.