Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0010899

Physical Location & Seq

Genomic Coordinates Forward (+)

20430025 .. 20432315

Gene Structure 2291 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1246200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1098 bp

ATGGCTCCTCCTGCTACTACGCTGACATCCATTGCGCATGAGAAAACCCTACAACAAAAATTCGTCCGAGACGAAGACGAGCGTCCAAAGGTTGCCTACAACGAATTCAGCAACGAAATTCCGATCATCTCGCTTGCCGGGATCGATGAGGTTGAAGGCCGCCGGGCCGAGATTTGCAAGAAGATTGTGGAAGCTTGTGAGGACTGGGGTATTTTCCAGATTGTTGATCATGGAGTTGATGCCGAGCTCATATCGGAAATGACCGGTCTCGCCAAAGAGTTCTTTGATTTGCCATCGGAGGAGAAGCTCCGCTTCGACATGTCCGGTGGCAAAAAGGGTGGATTCATCGTGTCCAGTCATTTACAGGGAGAAGCTGTGCAAGATTGGCGTGAAATTGTGACCTACTTTTCATACCCGATTCGCCACCGGGACTACTCGAGGTGGCCGGACAAGCCAGAGGCATGGAGGGAGGTGACGAAGAAGTACAGCGACGAGCTGATGGGGCTGGCATGCAAGCTCTTGGGGGTTTTATCAGAAGCCATGGGGTTGGATACAGAGGCATTGACAAAGGCATGTGTGGACATGGACCAAAAAGTGGTGGTGAATTTCTATCCGAAGTGCCCTCAGCCCGACCTAACTCTTGGCCTCAAGCGCCACACGGACCCGGGCACAATTACCCTTTTGCTTCAGGACCAAGTTGGTGGCCTTCAGGCTACTAGGGATGATGGGAAGACATGGATCACCGTTCAACCAGTGGAAGGAGCTTTTGTGGTCAATCTCGGAGATCATGGTCATTTTCTGAGCAATGGGAGGTTCAAGAATGCTGATCACCAAGCAGTGGTGAACTCAAACAGCAGCAGGCTGTCCATAGCCACATTCCAGAACCCAGCTCAAGATGCAATAGTGTATCCACTCAGTGTGAGGGAGGGAGAGAAGCCGATTCTCGAGGCGCCGATCACCTACACCGAGATGTACAAGAAGAAGATGAGCAAAGATCTTGAGCTTGCCAGGCTGAAAAAGCTGGCCAAGGAACAGCAACTGCAGGACTTGGAGAAAGCCAAAGTGGAGACAAAGCCAGCGGACGACATATTTGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009058 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009812 GO:0009813 GO:0010224 GO:0016491 GO:0016705 GO:0016706 GO:0045486 GO:0050896 GO:0051213 GO:0055114 GO:0071704 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00941 ko01100 ko01110 map00941 map01100 map01110

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

366

Amino Acids

41.0

Weight (kDa)

5.29

Isoelectric Point (pI)

38.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	40 - 150	2.1e-24	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	196 - 296	1.5e-31	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010899)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G51240 AT3G51240
fragaria_vesca	FvH4_1g11810
malus_domestica	MD02G1132200.v1.1 MD15G1246200.v1.1
prunus_persica	Prupe.7G168300_v2.0.a1 Prupe.7G168300_v2.0.a1
pyrus_communis	pycom02g10320 pycom15g21590
rosa_chinensis	RchiOBHm_Chr2g0099421
rosa_laevigata	RLG00000016880
rosa_multiflora	Rmu_co8440373.1_g000001 Rmu_sc0002487.1_g000013
rosa_roxburghii	Rroxscaffold_2G00143090
rosa_rugosa	Rorug02G0079600
rosa_samantha	Rh2AG129400 Rh2BG132300 Rh2CG133200 Rh2DG133500
rosa_wichuraiana	Rw2G009960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	36
AccB1I	GGYRCC	1 cut(s)	949
AccB7I	CCANNNNNTGG	2 cut(s)	595, 838
AciI	CCGC	3 cut(s)	160, 310, 1079
AclWI	GGATC	2 cut(s)	149, 746
AcoI	YGGCCR	2 cut(s)	443, 1023
AcsI	RAATTY	4 cut(s)	59, 104, 117, 604
AcuI	CTGAAG	2 cut(s)	671, 692
AcyI	GRCGYC	1 cut(s)	950
AdeI	CACNNNGTG	1 cut(s)	917
AfaI	GTAC	2 cut(s)	485, 974
AfiI	CCNNNNNNNGG	3 cut(s)	595, 758, 838
AflIII	ACRYGT	1 cut(s)	318
AgeI	ACCGGT	1 cut(s)	263
AgsI	TTSAA	3 cut(s)	155, 749, 817
AjnI	CCWGG	1 cut(s)	1007
Alw21I	GWGCWC	1 cut(s)	249
Alw26I	GTCTC	3 cut(s)	63, 272, 1061
AlwI	GGATC	2 cut(s)	149, 746
AlwNI	CAGNNNCTG	1 cut(s)	1039
Ama87I	CYCGRG	3 cut(s)	436, 664, 944
AoxI	GGCC	6 cut(s)	157, 165, 443, 643, 703, 1023
ApeKI	GCWGC	1 cut(s)	855
ApoI	RAATTY	4 cut(s)	59, 104, 117, 604
ArsI	GACNNNNNNTTYG	1 cut(s)	1073
AsiGI	ACCGGT	1 cut(s)	263
AspLEI	GCGC	3 cut(s)	37, 654, 952
AspS9I	GGNCC	4 cut(s)	165, 586, 661, 691
AsuC2I	CCSGG	5 cut(s)	139, 164, 428, 665, 666
AsuHPI	GGTGA	6 cut(s)	484, 613, 733, 821, 853, 949
AvaI	CYCGRG	3 cut(s)	436, 664, 944
AvaII	GGWCC	3 cut(s)	586, 661, 691
BaeGI	GKGCMC	2 cut(s)	623, 671
BalI	TGGCCA	1 cut(s)	1025
BanI	GGYRCC	1 cut(s)	949
BanII	GRGCYC	1 cut(s)	249
BbsI	GAAGAC	2 cut(s)	81, 737
Bbv12I	GWGCWC	1 cut(s)	249
BbvCI	CCTCAGC	1 cut(s)	624
BbvI	GCAGC	1 cut(s)	867
BccI	CCATC	3 cut(s)	301, 493, 719
BcgI	CGANNNNNNTGC	1 cut(s)	1073
BciT130I	CCWGG	1 cut(s)	1009
BciVI	GTATCC	2 cut(s)	544, 918
BclI	TGATCA	2 cut(s)	226, 826
BcnI	CCSGG	5 cut(s)	139, 164, 428, 665, 666
BcoDI	GTCTC	3 cut(s)	63, 272, 1061
BfaI	CTAG	1 cut(s)	717
BfmI	CTRYAG	1 cut(s)	1040
BfoI	RGCGCY	2 cut(s)	655, 953
BfuI	GTATCC	2 cut(s)	544, 918
BglII	AGATCT	1 cut(s)	994
BisI	GCNGC	2 cut(s)	160, 856
BlsI	GCNGC	2 cut(s)	161, 857
Bme1390I	CCNGG	6 cut(s)	139, 164, 428, 665, 666, 1009
Bme18I	GGWCC	3 cut(s)	586, 661, 691
BmeT110I	CYCGRG	3 cut(s)	436, 664, 944
BmgT120I	GGNCC	4 cut(s)	165, 586, 661, 691
BmiI	GGNNCC	3 cut(s)	6, 663, 951
BmrFI	CCNGG	6 cut(s)	139, 164, 428, 665, 666, 1009
BmrI	ACTGGG	1 cut(s)	214
BmsI	GCATC	2 cut(s)	229, 886
BmuI	ACTGGG	1 cut(s)	214
BoxI	GACNNNNGTC	1 cut(s)	81
BpiI	GAAGAC	2 cut(s)	81, 737
Bpu10I	CCTNAGC	1 cut(s)	624
BpuEI	CTTGAG	3 cut(s)	632, 876, 1019
BpuMI	CCSGG	5 cut(s)	139, 164, 428, 665, 666
Bsa29I	ATCGAT	1 cut(s)	144
BsaHI	GRCGYC	1 cut(s)	950
BsaI	GGTCTC	1 cut(s)	272
BsaJI	CCNNGG	3 cut(s)	540, 664, 1026
BsaWI	WCCGGW	2 cut(s)	263, 323
BsaXI	ACNNNNNCTCC	4 cut(s)	360, 390, 753, 783
Bsc4I	CCNNNNNNNGG	3 cut(s)	595, 758, 838
Bse118I	RCCGGY	1 cut(s)	263
Bse1I	ACTGG	3 cut(s)	209, 354, 752
Bse3DI	GCAATG	2 cut(s)	30, 811
BseBI	CCWGG	1 cut(s)	1009
BseCI	ATCGAT	1 cut(s)	144
BseDI	CCNNGG	3 cut(s)	540, 664, 1026
BseGI	GGATG	2 cut(s)	26, 727
BseLI	CCNNNNNNNGG	3 cut(s)	595, 758, 838
BseMI	GCAATG	2 cut(s)	30, 811
BseMII	CTCAG	3 cut(s)	638, 791, 928
BseNI	ACTGG	3 cut(s)	209, 354, 752
BseRI	GAGGAG	1 cut(s)	314
BseSI	GKGCMC	2 cut(s)	623, 671
BseXI	GCAGC	1 cut(s)	867
BseYI	CCCAGC	1 cut(s)	886
BshFI	GGCC	6 cut(s)	159, 167, 445, 645, 705, 1025
BshNI	GGYRCC	1 cut(s)	949
BshTI	ACCGGT	1 cut(s)	263
BshVI	ATCGAT	1 cut(s)	144
BsiHKAI	GWGCWC	1 cut(s)	249
BsiHKCI	CYCGRG	3 cut(s)	436, 664, 944
BsiSI	CCGG	7 cut(s)	138, 163, 264, 324, 427, 446, 665
BslFI	GGGAC	1 cut(s)	443
BslI	CCNNNNNNNGG	3 cut(s)	595, 758, 838
BsmAI	GTCTC	3 cut(s)	63, 272, 1061
BsmBI	CGTCTC	1 cut(s)	63
BsmFI	GGGAC	1 cut(s)	443
BsmI	GAATGC	1 cut(s)	826
BsnI	GGCC	6 cut(s)	159, 167, 445, 645, 705, 1025
Bso31I	GGTCTC	1 cut(s)	272
BsoBI	CYCGRG	3 cut(s)	436, 664, 944
Bsp1286I	GDGCHC	3 cut(s)	249, 623, 671
Bsp1407I	TGTACA	1 cut(s)	972
Bsp143I	GATC	8 cut(s)	123, 141, 226, 738, 784, 826, 954, 994
Bsp19I	CCATGG	1 cut(s)	540
BspACI	CCGC	3 cut(s)	160, 310, 1079
BspANI	GGCC	6 cut(s)	159, 167, 445, 645, 705, 1025
BspCNI	CTCAG	3 cut(s)	637, 792, 927
BspDI	ATCGAT	1 cut(s)	144
BspLI	GGNNCC	3 cut(s)	6, 663, 951
BspMAI	CTGCAG	1 cut(s)	1044
BspPI	GGATC	2 cut(s)	149, 746
BspT107I	GGYRCC	1 cut(s)	949
BspTNI	GGTCTC	1 cut(s)	272
BsrDI	GCAATG	2 cut(s)	30, 811
BsrFI	RCCGGY	1 cut(s)	263
BsrGI	TGTACA	1 cut(s)	972
BsrI	ACTGG	3 cut(s)	209, 354, 752
BssAI	RCCGGY	1 cut(s)	263
BssECI	CCNNGG	3 cut(s)	540, 664, 1026
BssMI	GATC	8 cut(s)	123, 141, 226, 738, 784, 826, 954, 994
BssNI	GRCGYC	1 cut(s)	950
BssT1I	CCWWGG	2 cut(s)	540, 1026
Bst2UI	CCWGG	1 cut(s)	1009
Bst4CI	ACNGT	1 cut(s)	745
BstACI	GRCGYC	1 cut(s)	950
BstAUI	TGTACA	1 cut(s)	972
BstC8I	GCNNGC	8 cut(s)	135, 507, 511, 515, 860, 1005, 1023, 1077
BstDEI	CTNAG	4 cut(s)	624, 800, 914, 1095
BstDSI	CCRYGG	1 cut(s)	540
BstF5I	GGATG	2 cut(s)	26, 727
BstH2I	RGCGCY	2 cut(s)	655, 953
BstHHI	GCGC	3 cut(s)	37, 654, 952
BstKTI	GATC	8 cut(s)	126, 144, 229, 741, 787, 829, 957, 997
BstMAI	GTCTC	3 cut(s)	63, 272, 1061
BstMBI	GATC	8 cut(s)	123, 141, 226, 738, 784, 826, 954, 994
BstMWI	GCNNNNNNNGC	8 cut(s)	11, 385, 451, 502, 651, 896, 1009, 1018
BstNI	CCWGG	1 cut(s)	1009
BstNSI	RCATGY	3 cut(s)	322, 513, 576
BstPAI	GACNNNNGTC	1 cut(s)	81
BstSCI	CCNGG	6 cut(s)	137, 162, 426, 663, 664, 1007
BstSFI	CTRYAG	1 cut(s)	1040
BstSLI	GKGCMC	2 cut(s)	623, 671
BstV1I	GCAGC	1 cut(s)	867
BstV2I	GAAGAC	2 cut(s)	81, 737
BstX2I	RGATCY	1 cut(s)	994
BstXI	CCANNNNNNTGG	3 cut(s)	462, 547, 701
BstYI	RGATCY	1 cut(s)	994
Bsu15I	ATCGAT	1 cut(s)	144
BsuI	GTATCC	2 cut(s)	544, 918
BsuRI	GGCC	6 cut(s)	159, 167, 445, 645, 705, 1025
BsuTUI	ATCGAT	1 cut(s)	144
BtgI	CCRYGG	1 cut(s)	540
BtsCI	GGATG	2 cut(s)	26, 727
BtsI	GCAGTG	1 cut(s)	843
BtsIMutI	CAGTG	3 cut(s)	759, 843, 922
Cac8I	GCNNGC	8 cut(s)	135, 507, 511, 515, 860, 1005, 1023, 1077
CaiI	CAGNNNCTG	1 cut(s)	1039
CfoI	GCGC	3 cut(s)	37, 654, 952
Cfr10I	RCCGGY	1 cut(s)	263
Cfr13I	GGNCC	4 cut(s)	165, 586, 661, 691
Cfr9I	CCCGGG	1 cut(s)	664
ClaI	ATCGAT	1 cut(s)	144
CseI	GACGC	1 cut(s)	71
Csp6I	GTAC	2 cut(s)	484, 973
CspAI	ACCGGT	1 cut(s)	263
CspCI	CAANNNNNGTGG	2 cut(s)	319, 354
CviQI	GTAC	2 cut(s)	484, 973
DdeI	CTNAG	4 cut(s)	624, 800, 914, 1095
DinI	GGCGCC	1 cut(s)	951
DpnI	GATC	8 cut(s)	125, 143, 228, 740, 786, 828, 956, 996
DpnII	GATC	8 cut(s)	123, 141, 226, 738, 784, 826, 954, 994
DraIII	CACNNNGTG	1 cut(s)	917
EaeI	YGGCCR	2 cut(s)	443, 1023
Ecl136II	GAGCTC	1 cut(s)	247
Eco130I	CCWWGG	2 cut(s)	540, 1026
Eco24I	GRGCYC	1 cut(s)	249
Eco31I	GGTCTC	1 cut(s)	272
Eco47I	GGWCC	3 cut(s)	586, 661, 691
Eco53kI	GAGCTC	1 cut(s)	247
Eco57I	CTGAAG	2 cut(s)	671, 692
Eco88I	CYCGRG	3 cut(s)	436, 664, 944
EcoICRI	GAGCTC	1 cut(s)	247
EcoRI	GAATTC	1 cut(s)	104
EcoRII	CCWGG	1 cut(s)	1007
EcoT14I	CCWWGG	2 cut(s)	540, 1026
EcoT38I	GRGCYC	1 cut(s)	249
EgeI	GGCGCC	1 cut(s)	951
EheI	GGCGCC	1 cut(s)	951
ErhI	CCWWGG	2 cut(s)	540, 1026
Esp3I	CGTCTC	1 cut(s)	63
FalI	AAGNNNNNCTT	2 cut(s)	296, 328
FaqI	GGGAC	1 cut(s)	443
FbaI	TGATCA	2 cut(s)	226, 826
Fnu4HI	GCNGC	2 cut(s)	160, 856
FokI	GGATG	2 cut(s)	13, 734
FriOI	GRGCYC	1 cut(s)	249
Fsp4HI	GCNGC	2 cut(s)	160, 856
FspBI	CTAG	1 cut(s)	717
FspI	TGCGCA	1 cut(s)	36
GlaI	GCGC	3 cut(s)	36, 653, 951
GluI	GCNGC	2 cut(s)	160, 856
GsaI	CCCAGC	1 cut(s)	890
HaeII	RGCGCY	2 cut(s)	655, 953
HaeIII	GGCC	6 cut(s)	159, 167, 445, 645, 705, 1025
HapII	CCGG	7 cut(s)	138, 163, 264, 324, 427, 446, 665
HgaI	GACGC	1 cut(s)	71
HhaI	GCGC	3 cut(s)	37, 654, 952
Hin1I	GRCGYC	1 cut(s)	950
Hin6I	GCGC	3 cut(s)	35, 652, 950
HinP1I	GCGC	3 cut(s)	35, 652, 950
HindIII	AAGCTT	1 cut(s)	192
HinfI	GANTC	3 cut(s)	342, 418, 940
HpaII	CCGG	7 cut(s)	138, 163, 264, 324, 427, 446, 665
HphI	GGTGA	6 cut(s)	484, 613, 733, 821, 853, 949
Hpy166II	GTNNAC	2 cut(s)	580, 844
Hpy188I	TCNGA	8 cut(s)	68, 123, 256, 298, 535, 615, 782, 801
Hpy188III	TCNNGA	7 cut(s)	217, 689, 817, 880, 893, 944, 998
Hpy8I	GTNNAC	2 cut(s)	580, 844
Hpy99I	CGWCG	1 cut(s)	494
HpyAV	CCTTC	3 cut(s)	149, 716, 752
HpyCH4III	ACNGT	1 cut(s)	745
HpyCH4V	TGCA	5 cut(s)	177, 379, 513, 899, 1042
HpyF10VI	GCNNNNNNNGC	8 cut(s)	11, 385, 451, 502, 651, 896, 1009, 1018
HpyF3I	CTNAG	4 cut(s)	624, 800, 914, 1095
Hsp92I	GRCGYC	1 cut(s)	950
HspAI	GCGC	3 cut(s)	35, 652, 950
KasI	GGCGCC	1 cut(s)	949
Ksp22I	TGATCA	2 cut(s)	226, 826
Kzo9I	GATC	8 cut(s)	123, 141, 226, 738, 784, 826, 954, 994
LmnI	GCTCC	3 cut(s)	10, 312, 761
Lsp1109I	GCAGC	1 cut(s)	867
LweI	GCATC	2 cut(s)	229, 886
MaeI	CTAG	1 cut(s)	717
MaeIII	GTNAC	2 cut(s)	397, 472
MalI	GATC	8 cut(s)	125, 143, 228, 740, 786, 828, 956, 996
MboI	GATC	8 cut(s)	123, 141, 226, 738, 784, 826, 954, 994
MboII	GAAGA	6 cut(s)	86, 193, 490, 742, 991, 994
MflI	RGATCY	1 cut(s)	994
MhlI	GDGCHC	3 cut(s)	249, 623, 671
MlsI	TGGCCA	1 cut(s)	1025
MluCI	AATT	6 cut(s)	59, 104, 117, 393, 604, 672
MluNI	TGGCCA	1 cut(s)	1025
Mly113I	GGCGCC	1 cut(s)	950
MmeI	TCCRAC	1 cut(s)	528
Mox20I	TGGCCA	1 cut(s)	1025
MscI	TGGCCA	1 cut(s)	1025
MslI	CAYNNNNRTG	1 cut(s)	968
Msp20I	TGGCCA	1 cut(s)	1025
MspA1I	CMGCKG	1 cut(s)	1079
MspI	CCGG	7 cut(s)	138, 163, 264, 324, 427, 446, 665
MspR9I	CCNGG	6 cut(s)	139, 164, 428, 665, 666, 1009
Mva1269I	GAATGC	1 cut(s)	826
MvaI	CCWGG	1 cut(s)	1009
MwoI	GCNNNNNNNGC	8 cut(s)	11, 385, 451, 502, 651, 896, 1009, 1018
NarI	GGCGCC	1 cut(s)	950
NciI	CCSGG	5 cut(s)	139, 164, 428, 665, 666
NcoI	CCATGG	1 cut(s)	540
NdeII	GATC	8 cut(s)	123, 141, 226, 738, 784, 826, 954, 994
NlaIV	GGNNCC	3 cut(s)	6, 663, 951
NmeAIII	GCCGAG	2 cut(s)	193, 268
NmuCI	GTSAC	2 cut(s)	397, 472
NsbI	TGCGCA	1 cut(s)	36
NspI	RCATGY	3 cut(s)	322, 513, 576
PaeI	GCATGC	1 cut(s)	513
PaeR7I	CTCGAG	2 cut(s)	436, 944
PciI	ACATGT	1 cut(s)	318
PcsI	WCGNNNNNNNCGW	1 cut(s)	69
PctI	GAATGC	1 cut(s)	826
PfeI	GAWTC	3 cut(s)	342, 418, 940
PflMI	CCANNNNNTGG	2 cut(s)	595, 838
PinAI	ACCGGT	1 cut(s)	263
PkrI	GCNGC	2 cut(s)	161, 857
PluTI	GGCGCC	1 cut(s)	953
PscI	ACATGT	1 cut(s)	318
PshAI	GACNNNNGTC	1 cut(s)	81
Psp124BI	GAGCTC	1 cut(s)	249
Psp6I	CCWGG	1 cut(s)	1007
PspFI	CCCAGC	1 cut(s)	886
PspGI	CCWGG	1 cut(s)	1007
PspN4I	GGNNCC	3 cut(s)	6, 663, 951
PspPI	GGNCC	4 cut(s)	165, 586, 661, 691
PspXI	VCTCGAGB	1 cut(s)	436
PstI	CTGCAG	1 cut(s)	1044
PstNI	CAGNNNCTG	1 cut(s)	1039
PsuI	RGATCY	1 cut(s)	994
RsaI	GTAC	2 cut(s)	485, 974
RsaNI	GTAC	2 cut(s)	484, 973
RseI	CAYNNNNRTG	1 cut(s)	968
SacI	GAGCTC	1 cut(s)	249
SatI	GCNGC	2 cut(s)	160, 856
Sau3AI	GATC	8 cut(s)	123, 141, 226, 738, 784, 826, 954, 994
Sau96I	GGNCC	4 cut(s)	165, 586, 661, 691
ScrFI	CCNGG	6 cut(s)	139, 164, 428, 665, 666, 1009
SduI	GDGCHC	3 cut(s)	249, 623, 671
SfaNI	GCATC	2 cut(s)	229, 886
SfcI	CTRYAG	1 cut(s)	1040
SfoI	GGCGCC	1 cut(s)	951
Sfr274I	CTCGAG	2 cut(s)	436, 944
SinI	GGWCC	3 cut(s)	586, 661, 691
SlaI	CTCGAG	2 cut(s)	436, 944
SmaI	CCCGGG	1 cut(s)	666
SmiMI	CAYNNNNRTG	1 cut(s)	968
SmlI	CTYRAG	5 cut(s)	436, 647, 891, 944, 998
SmoI	CTYRAG	5 cut(s)	436, 647, 891, 944, 998
SphI	GCATGC	1 cut(s)	513
Sse9I	AATT	6 cut(s)	59, 104, 117, 393, 604, 672
SsiI	CCGC	3 cut(s)	160, 310, 1079
SspDI	GGCGCC	1 cut(s)	949
SspMI	CTAG	1 cut(s)	717
SstI	GAGCTC	1 cut(s)	249
StyD4I	CCNGG	6 cut(s)	137, 162, 426, 663, 664, 1007
StyI	CCWWGG	2 cut(s)	540, 1026
TaaI	ACNGT	1 cut(s)	745
TaqI	TCGA	4 cut(s)	144, 315, 437, 945
TasI	AATT	6 cut(s)	59, 104, 117, 393, 604, 672
TatI	WGTACW	2 cut(s)	483, 972
TauI	GCSGC	1 cut(s)	162
TfiI	GAWTC	3 cut(s)	342, 418, 940
TscAI	CASTG	3 cut(s)	759, 843, 922
TseFI	GTSAC	2 cut(s)	397, 472
TseI	GCWGC	1 cut(s)	855
Tsp45I	GTSAC	2 cut(s)	397, 472
TspDTI	ATGAA	2 cut(s)	334, 399
TspGWI	ACGGA	1 cut(s)	674
TspMI	CCCGGG	1 cut(s)	664
TspRI	CASTG	3 cut(s)	759, 843, 922
Van91I	CCANNNNNTGG	2 cut(s)	595, 838
VpaK11BI	GGWCC	3 cut(s)	586, 661, 691
XapI	RAATTY	4 cut(s)	59, 104, 117, 604
XceI	RCATGY	3 cut(s)	322, 513, 576
XhoI	CTCGAG	2 cut(s)	436, 944
XmaI	CCCGGG	1 cut(s)	664
XspI	CTAG	1 cut(s)	717

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.