RLG00000016880

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0010899
Physical Location & Seq
Reverse (-)
10919855 .. 10921358
1504 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016880

Sequence Viewer

Length: 810 bp
ATGGCCCCTACTCCTACTACTCTGACCGCCATAGCGGGGGAGAAGACCCTCCAACAGAGCTTCGTCCGCGACGAAGACGAGCGCCCCAAGGTGGCCTACAACCAATTCAGCAACGAAATTCCGATCATTTCCCTCTCCGGCATCGAAGAGGTCGACGGCCGCCGCGCTGAGATATGCAAGAAGATCGTCGAGGCCTGCGAGGACTGGGGCGTTTTCCAGATTGTTGATCACGGCATCGACTCCAAGCTCATCTCGGAAATGACCCGTCTCGCCAGAGAGTTCTTCGCTTTGCCGCCGGAGGAAAAGCTCCGGTTCGACATGACCGGTGGCAAAAAGGGTGGCTTCATCGTCTCCAGCCACTTACAGGGAGAGGCTGTGCAGGACTGGCGCGAGATTGTGACCTACTTCTCATACCCGGTGCGCCACCGCGACTACTCGAGGTGGCCCGACAAGCCGGAGGGATGGAGAGATGTGACCAAGCAGTACAGTGACGAGCTGATGGGCTTGGCATGCAAGCTATTGGAGGTCTTATCAGAGGCCATGGGTTTAGAGAAGGAGGCGTTGACCAAGGCATGTGTGGACATGGACCAAAAGGTTGTGGTGAATTACTACCCGAAATGCCCCCAACCCGACCTCACTCTCGGACTCAAGCGCCACACAGATCCGGGTACAATTACCCTTTTGCTCCAGGACCAAGTCGGTGGACTTCAGGCCACCAGGGACGGCGGAAAGACGTGGATCACCGTTCAACCCGTGGAAGGAGCCTTTGTCGTCAACCTTGGAGATCATGGACATGTGAGTATATTTTGA

Protein Analysis

270

Amino Acids

30.22

Weight (kDa)

5.18

Isoelectric Point (pI)

42.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 40 - 150 9.4e-25 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 196 - 263 1.7e-21 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010899)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51240 AT3G51240
fragaria_vesca FvH4_1g11810
malus_domestica MD02G1132200.v1.1 MD15G1246200.v1.1
prunus_persica Prupe.7G168300_v2.0.a1 Prupe.7G168300_v2.0.a1
pyrus_communis pycom02g10320 pycom15g21590
rosa_chinensis RchiOBHm_Chr2g0099421
rosa_laevigata RLG00000016880
rosa_multiflora Rmu_co8440373.1_g000001 Rmu_sc0002487.1_g000013
rosa_roxburghii Rroxscaffold_2G00143090
rosa_rugosa Rorug02G0079600
rosa_samantha Rh2AG129400 Rh2BG132300 Rh2CG133200 Rh2DG133500
rosa_wichuraiana Rw2G009960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 153
AccII CGCG 4 cut(s) 69, 165, 390, 429
AciI CCGC 8 cut(s) 27, 35, 67, 160, 163, 293, 427, 726
AclWI GGATC 2 cut(s) 656, 746
AcoI YGGCCR 1 cut(s) 157
AcsI RAATTY 1 cut(s) 117
AcuI CTGAAG 1 cut(s) 692
AfaI GTAC 2 cut(s) 485, 670
AfiI CCNNNNNNNGG 4 cut(s) 36, 91, 364, 758
AflIII ACRYGT 1 cut(s) 793
AgeI ACCGGT 1 cut(s) 323
AgsI TTSAA 1 cut(s) 749
AjiI CACGTC 1 cut(s) 735
AjnI CCWGG 2 cut(s) 687, 716
AluBI AGCT 5 cut(s) 60, 247, 307, 496, 517
AluI AGCT 5 cut(s) 60, 247, 307, 496, 517
Alw26I GTCTC 2 cut(s) 272, 355
AlwI GGATC 2 cut(s) 656, 746
Ama87I CYCGRG 1 cut(s) 436
AoxI GGCC 7 cut(s) 3, 93, 157, 192, 443, 537, 711
ApoI RAATTY 1 cut(s) 117
AsiGI ACCGGT 1 cut(s) 323
AspLEI GCGC 5 cut(s) 84, 167, 390, 423, 654
AspS9I GGNCC 4 cut(s) 4, 444, 586, 691
AsuC2I CCSGG 2 cut(s) 416, 666
AsuHPI GGTGA 2 cut(s) 613, 733
AvaI CYCGRG 1 cut(s) 436
AvaII GGWCC 2 cut(s) 586, 691
BbsI GAAGAC 2 cut(s) 50, 81
BccI CCATC 2 cut(s) 456, 493
BceAI ACGGC 3 cut(s) 172, 247, 739
BcgI CGANNNNNNTGC 2 cut(s) 166, 200
BciT130I CCWGG 2 cut(s) 689, 718
BclI TGATCA 1 cut(s) 226
BcnI CCSGG 2 cut(s) 416, 666
BcoDI GTCTC 2 cut(s) 272, 355
BfoI RGCGCY 2 cut(s) 85, 655
BisI GCNGC 3 cut(s) 160, 163, 293
BlsI GCNGC 3 cut(s) 161, 164, 294
Bme1390I CCNGG 4 cut(s) 416, 666, 689, 718
Bme18I GGWCC 2 cut(s) 586, 691
BmeT110I CYCGRG 1 cut(s) 436
BmgBI CACGTC 1 cut(s) 735
BmgT120I GGNCC 4 cut(s) 4, 444, 586, 691
BmiI GGNNCC 2 cut(s) 6, 763
BmrFI CCNGG 4 cut(s) 416, 666, 689, 718
BmrI ACTGGG 1 cut(s) 214
BmsI GCATC 2 cut(s) 150, 243
BmuI ACTGGG 1 cut(s) 214
BpiI GAAGAC 2 cut(s) 50, 81
BpmI CTGGAG 2 cut(s) 337, 671
BpuEI CTTGAG 1 cut(s) 632
BpuMI CCSGG 2 cut(s) 416, 666
BsaJI CCNNGG 6 cut(s) 87, 540, 567, 717, 753, 778
BsaWI WCCGGW 2 cut(s) 309, 323
BsaXI ACNNNNNCTCC 4 cut(s) 360, 390, 753, 783
Bsc4I CCNNNNNNNGG 4 cut(s) 36, 91, 364, 758
Bse118I RCCGGY 1 cut(s) 323
Bse1I ACTGG 2 cut(s) 209, 389
BseBI CCWGG 2 cut(s) 689, 718
BseDI CCNNGG 6 cut(s) 87, 540, 567, 717, 753, 778
BseGI GGATG 1 cut(s) 467
BseLI CCNNNNNNNGG 4 cut(s) 36, 91, 364, 758
BseMII CTCAG 1 cut(s) 159
BseNI ACTGG 2 cut(s) 209, 389
BseX3I CGGCCG 1 cut(s) 157
BsgI GTGCAG 1 cut(s) 398
Bsh1236I CGCG 4 cut(s) 69, 165, 390, 429
Bsh1285I CGRYCG 1 cut(s) 160
BshFI GGCC 7 cut(s) 5, 95, 159, 194, 445, 539, 713
BshTI ACCGGT 1 cut(s) 323
BsiEI CGRYCG 1 cut(s) 160
BsiHKCI CYCGRG 1 cut(s) 436
BsiSI CCGG 7 cut(s) 138, 296, 310, 324, 416, 455, 665
BslFI GGGAC 1 cut(s) 734
BslI CCNNNNNNNGG 4 cut(s) 36, 91, 364, 758
BsmAI GTCTC 2 cut(s) 272, 355
BsmBI CGTCTC 2 cut(s) 272, 355
BsmFI GGGAC 1 cut(s) 734
BsnI GGCC 7 cut(s) 5, 95, 159, 194, 445, 539, 713
BsoBI CYCGRG 1 cut(s) 436
Bsp143I GATC 6 cut(s) 123, 183, 226, 661, 738, 784
Bsp19I CCATGG 1 cut(s) 540
BspACI CCGC 8 cut(s) 27, 35, 67, 160, 163, 293, 427, 726
BspANI GGCC 7 cut(s) 5, 95, 159, 194, 445, 539, 713
BspCNI CTCAG 1 cut(s) 160
BspFNI CGCG 4 cut(s) 69, 165, 390, 429
BspLI GGNNCC 2 cut(s) 6, 763
BspPI GGATC 2 cut(s) 656, 746
BsrFI RCCGGY 1 cut(s) 323
BsrI ACTGG 2 cut(s) 209, 389
BssAI RCCGGY 1 cut(s) 323
BssECI CCNNGG 6 cut(s) 87, 540, 567, 717, 753, 778
BssMI GATC 6 cut(s) 123, 183, 226, 661, 738, 784
BssT1I CCWWGG 4 cut(s) 87, 540, 567, 778
Bst2UI CCWGG 2 cut(s) 689, 718
Bst4CI ACNGT 2 cut(s) 488, 745
Bst6I CTCTTC 1 cut(s) 141
BstC8I GCNNGC 3 cut(s) 196, 511, 515
BstDEI CTNAG 1 cut(s) 168
BstDSI CCRYGG 2 cut(s) 540, 753
BstF5I GGATG 1 cut(s) 467
BstFNI CGCG 4 cut(s) 69, 165, 390, 429
BstH2I RGCGCY 2 cut(s) 85, 655
BstHHI GCGC 5 cut(s) 84, 167, 390, 423, 654
BstKTI GATC 6 cut(s) 126, 186, 229, 664, 741, 787
BstMAI GTCTC 2 cut(s) 272, 355
BstMBI GATC 6 cut(s) 123, 183, 226, 661, 738, 784
BstMCI CGRYCG 1 cut(s) 160
BstMWI GCNNNNNNNGC 4 cut(s) 66, 385, 451, 510
BstNI CCWGG 2 cut(s) 689, 718
BstNSI RCATGY 3 cut(s) 513, 576, 797
BstSCI CCNGG 4 cut(s) 414, 664, 687, 716
BstUI CGCG 4 cut(s) 69, 165, 390, 429
BstV2I GAAGAC 2 cut(s) 50, 81
BstX2I RGATCY 1 cut(s) 661
BstXI CCANNNNNNTGG 1 cut(s) 701
BstYI RGATCY 1 cut(s) 661
BstZI CGGCCG 1 cut(s) 157
BsuRI GGCC 7 cut(s) 5, 95, 159, 194, 445, 539, 713
BtgI CCRYGG 2 cut(s) 540, 753
BtrI CACGTC 1 cut(s) 735
BtsCI GGATG 1 cut(s) 467
BtsIMutI CAGTG 1 cut(s) 493
Cac8I GCNNGC 3 cut(s) 196, 511, 515
CfoI GCGC 5 cut(s) 84, 167, 390, 423, 654
Cfr10I RCCGGY 1 cut(s) 323
Cfr13I GGNCC 4 cut(s) 4, 444, 586, 691
Csp6I GTAC 2 cut(s) 484, 669
CspAI ACCGGT 1 cut(s) 323
CspCI CAANNNNNGTGG 2 cut(s) 319, 354
CviAII CATG 7 cut(s) 319, 510, 541, 573, 583, 788, 794
CviQI GTAC 2 cut(s) 484, 669
DdeI CTNAG 1 cut(s) 168
DpnI GATC 6 cut(s) 125, 185, 228, 663, 740, 786
DpnII GATC 6 cut(s) 123, 183, 226, 661, 738, 784
EaeI YGGCCR 1 cut(s) 157
EagI CGGCCG 1 cut(s) 157
Eam1104I CTCTTC 1 cut(s) 141
EarI CTCTTC 1 cut(s) 141
EciI GGCGGA 1 cut(s) 741
EclXI CGGCCG 1 cut(s) 157
Eco130I CCWWGG 4 cut(s) 87, 540, 567, 778
Eco147I AGGCCT 1 cut(s) 194
Eco47I GGWCC 2 cut(s) 586, 691
Eco52I CGGCCG 1 cut(s) 157
Eco57I CTGAAG 1 cut(s) 692
Eco88I CYCGRG 1 cut(s) 436
EcoRII CCWGG 2 cut(s) 687, 716
EcoT14I CCWWGG 4 cut(s) 87, 540, 567, 778
ErhI CCWWGG 4 cut(s) 87, 540, 567, 778
Esp3I CGTCTC 2 cut(s) 272, 355
FaeI CATG 7 cut(s) 322, 513, 544, 576, 586, 791, 797
FalI AAGNNNNNCTT 2 cut(s) 326, 358
FaqI GGGAC 1 cut(s) 734
FatI CATG 7 cut(s) 318, 509, 540, 572, 582, 787, 793
FauI CCCGC 1 cut(s) 28
FbaI TGATCA 1 cut(s) 226
FblI GTMKAC 1 cut(s) 153
Fnu4HI GCNGC 3 cut(s) 160, 163, 293
FokI GGATG 1 cut(s) 474
Fsp4HI GCNGC 3 cut(s) 160, 163, 293
GlaI GCGC 5 cut(s) 83, 166, 389, 422, 653
GluI GCNGC 3 cut(s) 160, 163, 293
GsuI CTGGAG 2 cut(s) 337, 671
HaeII RGCGCY 2 cut(s) 85, 655
HaeIII GGCC 7 cut(s) 5, 95, 159, 194, 445, 539, 713
HapII CCGG 7 cut(s) 138, 296, 310, 324, 416, 455, 665
HhaI GCGC 5 cut(s) 84, 167, 390, 423, 654
Hin1II CATG 7 cut(s) 322, 513, 544, 576, 586, 791, 797
Hin6I GCGC 5 cut(s) 82, 165, 388, 421, 652
HinP1I GCGC 5 cut(s) 82, 165, 388, 421, 652
HincII GTYRAC 3 cut(s) 154, 564, 775
HindII GTYRAC 3 cut(s) 154, 564, 775
HinfI GANTC 2 cut(s) 239, 645
HpaII CCGG 7 cut(s) 138, 296, 310, 324, 416, 455, 665
HphI GGTGA 2 cut(s) 613, 733
Hpy166II GTNNAC 5 cut(s) 154, 564, 580, 704, 775
Hpy188I TCNGA 5 cut(s) 24, 123, 256, 535, 644
Hpy188III TCNNGA 1 cut(s) 217
Hpy8I GTNNAC 5 cut(s) 154, 564, 580, 704, 775
Hpy99I CGWCG 3 cut(s) 74, 158, 191
HpyAV CCTTC 2 cut(s) 547, 752
HpyCH4III ACNGT 2 cut(s) 488, 745
HpyCH4IV ACGT 1 cut(s) 734
HpyCH4V TGCA 3 cut(s) 177, 379, 513
HpyF10VI GCNNNNNNNGC 4 cut(s) 66, 385, 451, 510
HpyF3I CTNAG 1 cut(s) 168
HpySE526I ACGT 1 cut(s) 734
Hsp92II CATG 7 cut(s) 322, 513, 544, 576, 586, 791, 797
HspAI GCGC 5 cut(s) 82, 165, 388, 421, 652
Ksp22I TGATCA 1 cut(s) 226
Kzo9I GATC 6 cut(s) 123, 183, 226, 661, 738, 784
LmnI GCTCC 3 cut(s) 312, 690, 761
LweI GCATC 2 cut(s) 150, 243
MaeII ACGT 1 cut(s) 734
MaeIII GTNAC 3 cut(s) 397, 472, 488
MalI GATC 6 cut(s) 125, 185, 228, 663, 740, 786
MboI GATC 6 cut(s) 123, 183, 226, 661, 738, 784
MboII GAAGA 5 cut(s) 55, 86, 158, 193, 274
MflI RGATCY 1 cut(s) 661
MluCI AATT 4 cut(s) 104, 117, 604, 672
MlyI GAGTC 2 cut(s) 233, 639
MmeI TCCRAC 1 cut(s) 76
MslI CAYNNNNRTG 1 cut(s) 792
MspI CCGG 7 cut(s) 138, 296, 310, 324, 416, 455, 665
MspR9I CCNGG 4 cut(s) 416, 666, 689, 718
MvaI CCWGG 2 cut(s) 689, 718
MvnI CGCG 4 cut(s) 69, 165, 390, 429
MwoI GCNNNNNNNGC 4 cut(s) 66, 385, 451, 510
NciI CCSGG 2 cut(s) 416, 666
NcoI CCATGG 1 cut(s) 540
NdeII GATC 6 cut(s) 123, 183, 226, 661, 738, 784
NlaIII CATG 7 cut(s) 322, 513, 544, 576, 586, 791, 797
NlaIV GGNNCC 2 cut(s) 6, 763
NmuCI GTSAC 3 cut(s) 397, 472, 488
NspI RCATGY 3 cut(s) 513, 576, 797
PaeI GCATGC 1 cut(s) 513
PaeR7I CTCGAG 1 cut(s) 436
PceI AGGCCT 1 cut(s) 194
PciI ACATGT 1 cut(s) 793
PcsI WCGNNNNNNNCGW 3 cut(s) 69, 150, 195
PflFI GACNNNGTC 1 cut(s) 695
PfoI TCCNGGA 1 cut(s) 687
PinAI ACCGGT 1 cut(s) 323
PkrI GCNGC 3 cut(s) 161, 164, 294
PleI GAGTC 2 cut(s) 233, 639
PpsI GAGTC 2 cut(s) 233, 639
PscI ACATGT 1 cut(s) 793
Psp6I CCWGG 2 cut(s) 687, 716
PspGI CCWGG 2 cut(s) 687, 716
PspN4I GGNNCC 2 cut(s) 6, 763
PspPI GGNCC 4 cut(s) 4, 444, 586, 691
PspXI VCTCGAGB 1 cut(s) 436
PsuI RGATCY 1 cut(s) 661
PsyI GACNNNGTC 1 cut(s) 695
RsaI GTAC 2 cut(s) 485, 670
RsaNI GTAC 2 cut(s) 484, 669
RseI CAYNNNNRTG 1 cut(s) 792
SalI GTCGAC 1 cut(s) 152
SatI GCNGC 3 cut(s) 160, 163, 293
Sau3AI GATC 6 cut(s) 123, 183, 226, 661, 738, 784
Sau96I GGNCC 4 cut(s) 4, 444, 586, 691
SchI GAGTC 2 cut(s) 233, 639
ScrFI CCNGG 4 cut(s) 416, 666, 689, 718
SfaNI GCATC 2 cut(s) 150, 243
Sfr274I CTCGAG 1 cut(s) 436
SinI GGWCC 2 cut(s) 586, 691
SlaI CTCGAG 1 cut(s) 436
SmiMI CAYNNNNRTG 1 cut(s) 792
SmlI CTYRAG 2 cut(s) 436, 647
SmoI CTYRAG 2 cut(s) 436, 647
SphI GCATGC 1 cut(s) 513
Sse9I AATT 4 cut(s) 104, 117, 604, 672
SseBI AGGCCT 1 cut(s) 194
SsiI CCGC 8 cut(s) 27, 35, 67, 160, 163, 293, 427, 726
StuI AGGCCT 1 cut(s) 194
StyD4I CCNGG 4 cut(s) 414, 664, 687, 716
StyI CCWWGG 4 cut(s) 87, 540, 567, 778
TaaI ACNGT 2 cut(s) 488, 745
TaiI ACGT 1 cut(s) 737
TaqI TCGA 6 cut(s) 144, 153, 189, 237, 315, 437
TasI AATT 4 cut(s) 104, 117, 604, 672
TatI WGTACW 1 cut(s) 483
TauI GCSGC 3 cut(s) 162, 165, 295
TscAI CASTG 1 cut(s) 493
TseFI GTSAC 3 cut(s) 397, 472, 488
Tsp45I GTSAC 3 cut(s) 397, 472, 488
TspDTI ATGAA 1 cut(s) 334
TspRI CASTG 1 cut(s) 493
Tth111I GACNNNGTC 1 cut(s) 695
VpaK11BI GGWCC 2 cut(s) 586, 691
XapI RAATTY 1 cut(s) 117
XceI RCATGY 3 cut(s) 513, 576, 797
XcmI CCANNNNNNNNNTGG 1 cut(s) 574
XhoI CTCGAG 1 cut(s) 436
XmiI GTMKAC 1 cut(s) 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.