MD15G1260600.v1.1

Zinc-finger homeodomain protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010518
Physical Location & Seq
Reverse (-)
22228452 .. 22229387
936 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1260600.v1.1.491

Sequence Viewer

Length: 936 bp
ATGGAACTGAGAGGGCAAGATAAGGTAATAGGGAGGATGCCAAGCACTCTTACCTATACTCCTCCTCCCCATAGGGATTCTTCTTCCTCCAAGCTTTCTTCTTCTCCGGATTTTCCCTCAGCAGGGCATCATGGTAACAACACCACACTCTTCACCCACCCACCTCATCAAACCCTAGACAGATCAAGAAGAGATCCTGACCCAACTCCAGTTATTGTCACCCCAAGTGCTGCAACAACAATTGCTAGCGGATCAAATTTCAAAGCACCACCAGCACCACCGCAACCGCAGCCAAGTACAGCAGCATCAAGAGTTAGGTATAGGGAGTGTTTGAAAAACCACGCCGCCAGCAGCGGCGGCCACGTGCTCGATGGATGCGGAGAGTTCATGCCGGGCGGAGATGAGGACACTCCGGGATCCCTAAAATGCGCAGCTTGCGAGTGTCATAGAAATTTTCACAGAAAAGAGATCGATGGCGACCATGTATCAAATAATTACTATGTCATAAACCAACAACACAACAGCCGTAGAGACTCGGCGATTACAAGATTGCCGGTGCTAATCTCCACACCACCGTCGCTTCCTGTTCATCATTCAGCGGCTAGGGGGCCTAGTCCGGCGATGATGATGACTTTTGGCAGCAGTGGTGGGGTGGCTGCTGAGTCCTCGAGCGAGGATCTGAACATGAATAATAATCTGTTTGGCGGTGCAAATTATGCGGCGGATGCACAAGGTTCGAAGAAGCGATTTAGGACGAAGTTCAGTCAAGAGCAGAAAGAAAAGATGACGGAGGTGGCGGAGAAGTTGGGGTGGAGGATCCAGAAACATGATGAGCAAGAGGTGGAGAAGTTGTGTTCTGAAGTGGGTATAAAGAGGCAGGTGTTCAAGGTATGGATGCACAATAACAAACAAGCCATGAAGAAGAAGCAAATGTAA

Protein Analysis

312

Amino Acids

34.12

Weight (kDa)

9.19

Isoelectric Point (pI)

63.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ZF-HD_dimer PF04770 105 - 157 1e-29 ZF-HD protein dimerisation region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 868
Acc16I TGCGCA 1 cut(s) 430
Acc36I ACCTGC 1 cut(s) 868
AccIII TCCGGA 1 cut(s) 106
AclWI GGATC 7 cut(s) 188, 259, 411, 424, 684, 811, 824
AcoI YGGCCR 1 cut(s) 358
AcsI RAATTY 2 cut(s) 256, 451
AcuI CTGAAG 1 cut(s) 879
AcvI CACGTG 1 cut(s) 364
AfaI GTAC 1 cut(s) 298
AfiI CCNNNNNNNGG 2 cut(s) 122, 123
AgsI TTSAA 3 cut(s) 262, 334, 886
AluBI AGCT 2 cut(s) 94, 434
AluI AGCT 2 cut(s) 94, 434
Alw21I GWGCWC 1 cut(s) 369
Alw26I GTCTC 1 cut(s) 525
AlwI GGATC 7 cut(s) 188, 259, 411, 424, 684, 811, 824
Ama87I CYCGRG 1 cut(s) 667
Aor13HI TCCGGA 1 cut(s) 106
AoxI GGCC 2 cut(s) 358, 608
ApeKI GCWGC 7 cut(s) 230, 289, 302, 351, 431, 639, 656
ApoI RAATTY 2 cut(s) 256, 451
AspLEI GCGC 1 cut(s) 431
AspS9I GGNCC 1 cut(s) 608
AsuC2I CCSGG 2 cut(s) 393, 414
AsuHPI GGTGA 2 cut(s) 145, 211
AsuII TTCGAA 1 cut(s) 737
AsuNHI GCTAGC 1 cut(s) 245
AvaI CYCGRG 1 cut(s) 667
BamHI GGATCC 2 cut(s) 416, 816
BbrPI CACGTG 1 cut(s) 364
Bbv12I GWGCWC 1 cut(s) 369
BbvCI CCTCAGC 1 cut(s) 118
BbvI GCAGC 7 cut(s) 217, 301, 314, 363, 443, 643, 651
BccI CCATC 2 cut(s) 365, 467
BceAI ACGGC 1 cut(s) 510
BcgI CGANNNNNNTGC 2 cut(s) 717, 751
BcnI CCSGG 2 cut(s) 393, 414
BcoDI GTCTC 1 cut(s) 525
BfaI CTAG 4 cut(s) 176, 246, 603, 612
BfuAI ACCTGC 1 cut(s) 868
Bme1390I CCNGG 2 cut(s) 393, 414
BmeT110I CYCGRG 1 cut(s) 667
BmgT120I GGNCC 1 cut(s) 608
BmiI GGNNCC 3 cut(s) 418, 609, 818
BmrFI CCNGG 2 cut(s) 393, 414
BmsI GCATC 6 cut(s) 27, 136, 314, 365, 715, 885
BmtI GCTAGC 1 cut(s) 249
BpmI CTGGAG 1 cut(s) 192
Bpu10I CCTNAGC 1 cut(s) 118
Bpu14I TTCGAA 1 cut(s) 737
BpuMI CCSGG 2 cut(s) 393, 414
Bsa29I ATCGAT 1 cut(s) 471
BsaAI YACGTR 1 cut(s) 364
BsaWI WCCGGW 1 cut(s) 106
BsaXI ACNNNNNCTCC 6 cut(s) 43, 49, 73, 79, 836, 866
Bsc4I CCNNNNNNNGG 2 cut(s) 122, 123
Bse118I RCCGGY 1 cut(s) 553
Bse1I ACTGG 1 cut(s) 209
BseAI TCCGGA 1 cut(s) 106
BseCI ATCGAT 1 cut(s) 471
BseGI GGATG 4 cut(s) 42, 380, 730, 900
BseLI CCNNNNNNNGG 2 cut(s) 122, 123
BseMII CTCAG 2 cut(s) 132, 651
BseNI ACTGG 1 cut(s) 209
BseRI GAGGAG 2 cut(s) 51, 54
BseXI GCAGC 7 cut(s) 217, 301, 314, 363, 443, 643, 651
BshFI GGCC 2 cut(s) 360, 610
BshVI ATCGAT 1 cut(s) 471
BsiHKAI GWGCWC 1 cut(s) 369
BsiHKCI CYCGRG 1 cut(s) 667
BsiSI CCGG 5 cut(s) 107, 392, 413, 554, 617
BslI CCNNNNNNNGG 2 cut(s) 122, 123
BsmAI GTCTC 1 cut(s) 525
BsnI GGCC 2 cut(s) 360, 610
BsoBI CYCGRG 1 cut(s) 667
Bsp119I TTCGAA 1 cut(s) 737
Bsp1286I GDGCHC 1 cut(s) 369
Bsp13I TCCGGA 1 cut(s) 106
Bsp143I GATC 7 cut(s) 182, 193, 251, 416, 468, 676, 816
BspANI GGCC 2 cut(s) 360, 610
BspCNI CTCAG 2 cut(s) 131, 652
BspDI ATCGAT 1 cut(s) 471
BspEI TCCGGA 1 cut(s) 106
BspLI GGNNCC 3 cut(s) 418, 609, 818
BspMI ACCTGC 1 cut(s) 868
BspOI GCTAGC 1 cut(s) 249
BspPI GGATC 7 cut(s) 188, 259, 411, 424, 684, 811, 824
BspT104I TTCGAA 1 cut(s) 737
BsrFI RCCGGY 1 cut(s) 553
BsrI ACTGG 1 cut(s) 209
BssAI RCCGGY 1 cut(s) 553
BssMI GATC 7 cut(s) 182, 193, 251, 416, 468, 676, 816
Bst4CI ACNGT 1 cut(s) 576
Bst6I CTCTTC 2 cut(s) 155, 184
BstAPI GCANNNNNTGC 1 cut(s) 716
BstBAI YACGTR 1 cut(s) 364
BstBI TTCGAA 1 cut(s) 737
BstC8I GCNNGC 3 cut(s) 247, 349, 436
BstDEI CTNAG 3 cut(s) 8, 118, 660
BstF5I GGATG 4 cut(s) 42, 380, 730, 900
BstHHI GCGC 1 cut(s) 431
BstKTI GATC 7 cut(s) 185, 196, 254, 419, 471, 679, 819
BstMAI GTCTC 1 cut(s) 525
BstMBI GATC 7 cut(s) 182, 193, 251, 416, 468, 676, 816
BstMWI GCNNNNNNNGC 6 cut(s) 272, 289, 357, 435, 716, 725
BstSCI CCNGG 2 cut(s) 391, 412
BstV1I GCAGC 7 cut(s) 217, 301, 314, 363, 443, 643, 651
BstX2I RGATCY 4 cut(s) 193, 416, 676, 816
BstYI RGATCY 4 cut(s) 193, 416, 676, 816
Bsu15I ATCGAT 1 cut(s) 471
BsuRI GGCC 2 cut(s) 360, 610
BsuTUI ATCGAT 1 cut(s) 471
BtgZI GCGATG 1 cut(s) 635
BtsCI GGATG 4 cut(s) 42, 380, 730, 900
BtsI GCAGTG 1 cut(s) 649
BtsIMutI CAGTG 1 cut(s) 649
BveI ACCTGC 1 cut(s) 868
Cac8I GCNNGC 3 cut(s) 247, 349, 436
CfoI GCGC 1 cut(s) 431
Cfr10I RCCGGY 1 cut(s) 553
Cfr13I GGNCC 1 cut(s) 608
ClaI ATCGAT 1 cut(s) 471
Csp6I GTAC 1 cut(s) 297
CviAII CATG 6 cut(s) 131, 388, 482, 685, 827, 916
CviJI RGCY 9 cut(s) 94, 292, 360, 434, 525, 602, 610, 656, 914
CviKI_1 RGCY 9 cut(s) 94, 292, 360, 434, 525, 602, 610, 656, 914
CviQI GTAC 1 cut(s) 297
DdeI CTNAG 3 cut(s) 8, 118, 660
DpnI GATC 7 cut(s) 184, 195, 253, 418, 470, 678, 818
DpnII GATC 7 cut(s) 182, 193, 251, 416, 468, 676, 816
EaeI YGGCCR 1 cut(s) 358
Eam1104I CTCTTC 2 cut(s) 155, 184
EarI CTCTTC 2 cut(s) 155, 184
EciI GGCGGA 3 cut(s) 411, 737, 812
Eco57I CTGAAG 1 cut(s) 879
Eco72I CACGTG 1 cut(s) 364
Eco88I CYCGRG 1 cut(s) 667
EcoO109I RGGNCCY 1 cut(s) 608
FaeI CATG 6 cut(s) 134, 391, 485, 688, 830, 919
FatI CATG 6 cut(s) 130, 387, 481, 684, 826, 915
FokI GGATG 4 cut(s) 49, 387, 737, 907
FspBI CTAG 4 cut(s) 176, 246, 603, 612
FspI TGCGCA 1 cut(s) 430
GlaI GCGC 1 cut(s) 430
GsuI CTGGAG 1 cut(s) 192
HaeIII GGCC 2 cut(s) 360, 610
HapII CCGG 5 cut(s) 107, 392, 413, 554, 617
HhaI GCGC 1 cut(s) 431
Hin1II CATG 6 cut(s) 134, 391, 485, 688, 830, 919
Hin6I GCGC 1 cut(s) 429
HinP1I GCGC 1 cut(s) 429
HindIII AAGCTT 1 cut(s) 92
HinfI GANTC 3 cut(s) 77, 533, 662
HpaII CCGG 5 cut(s) 107, 392, 413, 554, 617
HphI GGTGA 2 cut(s) 145, 211
Hpy188I TCNGA 2 cut(s) 681, 859
Hpy188III TCNNGA 6 cut(s) 107, 186, 197, 309, 767, 820
Hpy99I CGWCG 1 cut(s) 580
HpyCH4III ACNGT 1 cut(s) 576
HpyCH4IV ACGT 1 cut(s) 363
HpyCH4V TGCA 4 cut(s) 233, 710, 728, 898
HpyF10VI GCNNNNNNNGC 6 cut(s) 272, 289, 357, 435, 716, 725
HpyF3I CTNAG 3 cut(s) 8, 118, 660
HpySE526I ACGT 1 cut(s) 363
Hsp92II CATG 6 cut(s) 134, 391, 485, 688, 830, 919
HspAI GCGC 1 cut(s) 429
Kpn2I TCCGGA 1 cut(s) 106
Kzo9I GATC 7 cut(s) 182, 193, 251, 416, 468, 676, 816
Lsp1109I GCAGC 7 cut(s) 217, 301, 314, 363, 443, 643, 651
LweI GCATC 6 cut(s) 27, 136, 314, 365, 715, 885
MaeI CTAG 4 cut(s) 176, 246, 603, 612
MaeII ACGT 1 cut(s) 363
MaeIII GTNAC 2 cut(s) 134, 217
MalI GATC 7 cut(s) 184, 195, 253, 418, 470, 678, 818
MboI GATC 7 cut(s) 182, 193, 251, 416, 468, 676, 816
MboII GAAGA 9 cut(s) 72, 75, 90, 93, 142, 201, 751, 931, 934
MfeI CAATTG 1 cut(s) 240
MflI RGATCY 4 cut(s) 193, 416, 676, 816
MhlI GDGCHC 1 cut(s) 369
MluCI AATT 5 cut(s) 240, 256, 451, 493, 712
MlyI GAGTC 2 cut(s) 527, 671
MroI TCCGGA 1 cut(s) 106
MspA1I CMGCKG 2 cut(s) 354, 599
MspI CCGG 5 cut(s) 107, 392, 413, 554, 617
MspR9I CCNGG 2 cut(s) 393, 414
MunI CAATTG 1 cut(s) 240
MwoI GCNNNNNNNGC 6 cut(s) 272, 289, 357, 435, 716, 725
NciI CCSGG 2 cut(s) 393, 414
NdeII GATC 7 cut(s) 182, 193, 251, 416, 468, 676, 816
NheI GCTAGC 1 cut(s) 245
NlaIII CATG 6 cut(s) 134, 391, 485, 688, 830, 919
NlaIV GGNNCC 3 cut(s) 418, 609, 818
NmeAIII GCCGAG 1 cut(s) 515
NmuCI GTSAC 1 cut(s) 217
NsbI TGCGCA 1 cut(s) 430
NspV TTCGAA 1 cut(s) 737
PaeR7I CTCGAG 1 cut(s) 667
PaqCI CACCTGC 1 cut(s) 868
PfeI GAWTC 1 cut(s) 77
PfoI TCCNGGA 1 cut(s) 412
PleI GAGTC 2 cut(s) 527, 670
PmaCI CACGTG 1 cut(s) 364
PmlI CACGTG 1 cut(s) 364
PpsI GAGTC 2 cut(s) 527, 670
Ppu21I YACGTR 1 cut(s) 364
PspCI CACGTG 1 cut(s) 364
PspN4I GGNNCC 3 cut(s) 418, 609, 818
PspPI GGNCC 1 cut(s) 608
PspXI VCTCGAGB 1 cut(s) 667
PsuI RGATCY 4 cut(s) 193, 416, 676, 816
RsaI GTAC 1 cut(s) 298
RsaNI GTAC 1 cut(s) 297
Sau3AI GATC 7 cut(s) 182, 193, 251, 416, 468, 676, 816
Sau96I GGNCC 1 cut(s) 608
SchI GAGTC 2 cut(s) 527, 671
ScrFI CCNGG 2 cut(s) 393, 414
SduI GDGCHC 1 cut(s) 369
SfaNI GCATC 6 cut(s) 27, 136, 314, 365, 715, 885
Sfr274I CTCGAG 1 cut(s) 667
SfuI TTCGAA 1 cut(s) 737
SlaI CTCGAG 1 cut(s) 667
SmlI CTYRAG 1 cut(s) 667
SmoI CTYRAG 1 cut(s) 667
Sse9I AATT 5 cut(s) 240, 256, 451, 493, 712
SspMI CTAG 4 cut(s) 176, 246, 603, 612
StyD4I CCNGG 2 cut(s) 391, 412
TaaI ACNGT 1 cut(s) 576
TaiI ACGT 1 cut(s) 366
TaqI TCGA 4 cut(s) 369, 471, 668, 737
TasI AATT 5 cut(s) 240, 256, 451, 493, 712
TatI WGTACW 1 cut(s) 296
TauI GCSGC 5 cut(s) 347, 357, 360, 602, 722
TfiI GAWTC 1 cut(s) 77
TscAI CASTG 1 cut(s) 649
TseFI GTSAC 1 cut(s) 217
TseI GCWGC 7 cut(s) 230, 289, 302, 351, 431, 639, 656
Tsp45I GTSAC 1 cut(s) 217
TspDTI ATGAA 4 cut(s) 376, 578, 701, 932
TspGWI ACGGA 1 cut(s) 803
TspRI CASTG 1 cut(s) 649
XapI RAATTY 2 cut(s) 256, 451
XcmI CCANNNNNNNNNTGG 1 cut(s) 368
XhoI CTCGAG 1 cut(s) 667
XspI CTAG 4 cut(s) 176, 246, 603, 612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.