Rroxscaffold_2G00141340

Zinc-finger homeodomain protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0010518
Physical Location & Seq
Forward (+)
79657439 .. 79658506
1068 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00141340.1

Sequence Viewer

Length: 1068 bp
ATGGAGGTGAGCGGCCAAGATAAGGTTTTAGGGATGCCAACCACTTTGGGGTTCAATCCCCAAAATAGAGACTCTTCTTCTTCCAAGCTCTCTTCTTCATCTCTTCATCATCATGTTAACAACACATTAATCTTCAACCACCCCCAAACCCTAGACCCTTTTTATCAGGCTCCACCTCATCATCATCATCAGCCTCAGCAATCGAATCCATACAAACCCAGTAGAGATCCTGACCCAATCCCTATCCCAATCCCAATCCCTATCCCAAACCGAGATCCAAACCTATCACCAGCTGCTGTCTGCACCAGCCCAAGAGCAACAAGCGGCACACCAATTGCAAGCCGAGCAAACCACAGCTTCAAAGCAGCACCATCGGCACCGGCACCTGAGCCAATTACAGATTCGAAAGCAGTTAGATATCGAGAATGCTTGAAGAATCACGCAGCTAGCACTGGAGGGCATGTACTGGATGGCTGCGGAGAGTTCATGCCGAGCGGAGAGGAAGACAGCCCGGGAGGACTAAAATGTGCAGCGTGTGAGTGCCACAGGAATTTCCACAGGAAAGAGATCGAGGGTGAAACCCAACTAGTACATGTACCCAATAATTACCATGTCCTTAACCACCACAACAAAAACAGCCACAGCAGCCGGAATGCATCATCAGCACCTGTTCTATCCTCACTCCATCCACCACCTCCTGTTCATCATCATCATCAGTACCATCATTTCCCAGCCACTAGTCCTTCCGTGGCCGGATCATTTCCTCCTGGGATGATGACTTTTGGAGGTGGCGGAGGGGCGGCCGAGTCATCTAGTGAGGATCTCAACCACATCCACATGTACGAGCAGTCAAATCAGGCCGGGGGTTCGAAGAAGCGGTTTCGGACAAAGTTCAGTCAAGAGCAGAAGGACAAGATGATGGAGGTGGCAGAAAAGTTGGGATGGAGGATCCAGAAGCACGATGAGCAAGAAGTGCAGCAGTTGTGTTCTGAATTGGGTATAAGGAGACAGGTTTTCAAGGTATGGATGCACAATAATAAACAAGCCATGAAGAAGAAGCAACTGTAA

Protein Analysis

355

Amino Acids

39.21

Weight (kDa)

8.76

Isoelectric Point (pI)

70.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ZF-HD_dimer PF04770 138 - 190 1.1e-29 ZF-HD protein dimerisation region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 376, 382
AccBSI CCGCTC 2 cut(s) 12, 495
AciI CCGC 7 cut(s) 12, 324, 477, 495, 792, 800, 877
AclWI GGATC 6 cut(s) 221, 269, 763, 828, 943, 956
AcoI YGGCCR 3 cut(s) 13, 750, 801
AcsI RAATTY 1 cut(s) 550
AfaI GTAC 5 cut(s) 465, 591, 597, 719, 842
AfiI CCNNNNNNNGG 2 cut(s) 22, 48
AflIII ACRYGT 2 cut(s) 592, 837
AgsI TTSAA 5 cut(s) 55, 136, 361, 433, 1018
AhlI ACTAGT 2 cut(s) 586, 737
AjnI CCWGG 1 cut(s) 766
AjuI GAANNNNNNNTTGG 2 cut(s) 385, 417
AluBI AGCT 4 cut(s) 88, 293, 357, 446
AluI AGCT 4 cut(s) 88, 293, 357, 446
Alw26I GTCTC 2 cut(s) 63, 1000
AlwI GGATC 6 cut(s) 221, 269, 763, 828, 943, 956
AlwNI CAGNNNCTG 2 cut(s) 296, 668
Ama87I CYCGRG 1 cut(s) 511
AoxI GGCC 4 cut(s) 13, 750, 801, 858
ApeKI GCWGC 7 cut(s) 293, 365, 443, 474, 530, 645, 976
ApoI RAATTY 1 cut(s) 550
AseI ATTAAT 1 cut(s) 128
AsuC2I CCSGG 3 cut(s) 512, 513, 862
AsuHPI GGTGA 3 cut(s) 19, 279, 587
AsuII TTCGAA 2 cut(s) 404, 869
AsuNHI GCTAGC 1 cut(s) 446
AvaI CYCGRG 1 cut(s) 511
BamHI GGATCC 1 cut(s) 948
BanI GGYRCC 2 cut(s) 376, 382
BbsI GAAGAC 1 cut(s) 510
BbvCI CCTCAGC 1 cut(s) 195
BbvI GCAGC 7 cut(s) 280, 377, 455, 461, 542, 657, 988
BccI CCATC 6 cut(s) 379, 464, 693, 729, 913, 936
BcgI CGANNNNNNTGC 2 cut(s) 354, 388
BciT130I CCWGG 1 cut(s) 768
BcnI CCSGG 3 cut(s) 512, 513, 862
BcoDI GTCTC 2 cut(s) 63, 1000
BcuI ACTAGT 2 cut(s) 586, 737
BfaI CTAG 5 cut(s) 152, 447, 587, 738, 813
Bme1390I CCNGG 4 cut(s) 512, 513, 768, 862
BmeT110I CYCGRG 1 cut(s) 511
BmiI GGNNCC 4 cut(s) 171, 378, 384, 950
BmrFI CCNGG 4 cut(s) 512, 513, 768, 862
BmrI ACTGGG 1 cut(s) 213
BmsI GCATC 3 cut(s) 24, 665, 1017
BmtI GCTAGC 1 cut(s) 450
BmuI ACTGGG 1 cut(s) 213
BpiI GAAGAC 1 cut(s) 510
BpmI CTGGAG 1 cut(s) 474
Bpu10I CCTNAGC 2 cut(s) 195, 387
Bpu14I TTCGAA 2 cut(s) 404, 869
BpuMI CCSGG 3 cut(s) 512, 513, 862
BsaJI CCNNGG 4 cut(s) 511, 747, 767, 861
BsaXI ACNNNNNCTCC 2 cut(s) 447, 477
Bsc4I CCNNNNNNNGG 2 cut(s) 22, 48
Bse118I RCCGGY 1 cut(s) 379
Bse1I ACTGG 3 cut(s) 219, 457, 471
BseBI CCWGG 1 cut(s) 768
BseDI CCNNGG 4 cut(s) 511, 747, 767, 861
BseGI GGATG 7 cut(s) 39, 475, 685, 777, 831, 947, 1032
BseLI CCNNNNNNNGG 2 cut(s) 22, 48
BseMII CTCAG 2 cut(s) 209, 378
BseNI ACTGG 3 cut(s) 219, 457, 471
BseX3I CGGCCG 1 cut(s) 801
BseXI GCAGC 7 cut(s) 280, 377, 455, 461, 542, 657, 988
BseYI CCCAGC 1 cut(s) 730
BsgI GTGCAG 3 cut(s) 286, 549, 995
Bsh1285I CGRYCG 1 cut(s) 804
BshFI GGCC 4 cut(s) 15, 752, 803, 860
BshNI GGYRCC 2 cut(s) 376, 382
BsiEI CGRYCG 1 cut(s) 804
BsiHKCI CYCGRG 1 cut(s) 511
BsiSI CCGG 5 cut(s) 380, 512, 649, 753, 861
BslI CCNNNNNNNGG 2 cut(s) 22, 48
BsmAI GTCTC 2 cut(s) 63, 1000
BsmI GAATGC 2 cut(s) 431, 658
BsnI GGCC 4 cut(s) 15, 752, 803, 860
BsoBI CYCGRG 1 cut(s) 511
Bsp119I TTCGAA 2 cut(s) 404, 869
Bsp143I GATC 6 cut(s) 226, 274, 567, 755, 820, 948
BspACI CCGC 7 cut(s) 12, 324, 477, 495, 792, 800, 877
BspANI GGCC 4 cut(s) 15, 752, 803, 860
BspCNI CTCAG 2 cut(s) 208, 379
BspLI GGNNCC 4 cut(s) 171, 378, 384, 950
BspOI GCTAGC 1 cut(s) 450
BspPI GGATC 6 cut(s) 221, 269, 763, 828, 943, 956
BspT104I TTCGAA 2 cut(s) 404, 869
BspT107I GGYRCC 2 cut(s) 376, 382
BsrBI CCGCTC 2 cut(s) 12, 495
BsrFI RCCGGY 1 cut(s) 379
BsrI ACTGG 3 cut(s) 219, 457, 471
BssAI RCCGGY 1 cut(s) 379
BssECI CCNNGG 4 cut(s) 511, 747, 767, 861
BssMI GATC 6 cut(s) 226, 274, 567, 755, 820, 948
Bst2UI CCWGG 1 cut(s) 768
Bst4CI ACNGT 1 cut(s) 1065
Bst6I CTCTTC 3 cut(s) 79, 97, 108
BstAPI GCANNNNNTGC 1 cut(s) 973
BstBI TTCGAA 2 cut(s) 404, 869
BstC8I GCNNGC 2 cut(s) 340, 448
BstDEI CTNAG 2 cut(s) 195, 387
BstDSI CCRYGG 1 cut(s) 747
BstF5I GGATG 7 cut(s) 39, 475, 685, 777, 831, 947, 1032
BstKTI GATC 6 cut(s) 229, 277, 570, 758, 823, 951
BstMAI GTCTC 2 cut(s) 63, 1000
BstMBI GATC 6 cut(s) 226, 274, 567, 755, 820, 948
BstMCI CGRYCG 1 cut(s) 804
BstMWI GCNNNNNNNGC 6 cut(s) 344, 374, 645, 662, 964, 973
BstNI CCWGG 1 cut(s) 768
BstNSI RCATGY 3 cut(s) 464, 596, 841
BstSCI CCNGG 4 cut(s) 510, 511, 766, 860
BstV1I GCAGC 7 cut(s) 280, 377, 455, 461, 542, 657, 988
BstV2I GAAGAC 1 cut(s) 510
BstX2I RGATCY 4 cut(s) 226, 274, 820, 948
BstYI RGATCY 4 cut(s) 226, 274, 820, 948
BstZI CGGCCG 1 cut(s) 801
BsuRI GGCC 4 cut(s) 15, 752, 803, 860
BtgI CCRYGG 1 cut(s) 747
BtsCI GGATG 7 cut(s) 39, 475, 685, 777, 831, 947, 1032
BtsIMutI CAGTG 1 cut(s) 450
Cac8I GCNNGC 2 cut(s) 340, 448
CaiI CAGNNNCTG 2 cut(s) 296, 668
Cfr10I RCCGGY 1 cut(s) 379
Cfr9I CCCGGG 1 cut(s) 511
Csp6I GTAC 5 cut(s) 464, 590, 596, 718, 841
CviAII CATG 7 cut(s) 113, 461, 487, 593, 611, 838, 1048
CviQI GTAC 5 cut(s) 464, 590, 596, 718, 841
DdeI CTNAG 2 cut(s) 195, 387
DpnI GATC 6 cut(s) 228, 276, 569, 757, 822, 950
DpnII GATC 6 cut(s) 226, 274, 567, 755, 820, 948
EaeI YGGCCR 3 cut(s) 13, 750, 801
EagI CGGCCG 1 cut(s) 801
Eam1104I CTCTTC 3 cut(s) 79, 97, 108
EarI CTCTTC 3 cut(s) 79, 97, 108
EciI GGCGGA 1 cut(s) 807
EclXI CGGCCG 1 cut(s) 801
Eco32I GATATC 1 cut(s) 419
Eco52I CGGCCG 1 cut(s) 801
Eco88I CYCGRG 1 cut(s) 511
EcoRII CCWGG 1 cut(s) 766
EcoRV GATATC 1 cut(s) 419
EcoT22I ATGCAT 1 cut(s) 658
FaeI CATG 7 cut(s) 116, 464, 490, 596, 614, 841, 1051
FatI CATG 7 cut(s) 112, 460, 486, 592, 610, 837, 1047
FokI GGATG 7 cut(s) 46, 482, 672, 784, 818, 954, 1039
FspBI CTAG 5 cut(s) 152, 447, 587, 738, 813
GsaI CCCAGC 1 cut(s) 734
GsuI CTGGAG 1 cut(s) 474
HaeIII GGCC 4 cut(s) 15, 752, 803, 860
HapII CCGG 5 cut(s) 380, 512, 649, 753, 861
Hin1II CATG 7 cut(s) 116, 464, 490, 596, 614, 841, 1051
HincII GTYRAC 1 cut(s) 118
HindII GTYRAC 1 cut(s) 118
HinfI GANTC 5 cut(s) 71, 205, 401, 436, 806
HpaI GTTAAC 1 cut(s) 118
HpaII CCGG 5 cut(s) 380, 512, 649, 753, 861
HphI GGTGA 3 cut(s) 19, 279, 587
Hpy166II GTNNAC 1 cut(s) 118
Hpy188I TCNGA 2 cut(s) 885, 991
Hpy188III TCNNGA 4 cut(s) 230, 422, 899, 952
Hpy8I GTNNAC 1 cut(s) 118
HpyAV CCTTC 2 cut(s) 753, 901
HpyCH4III ACNGT 1 cut(s) 1065
HpyCH4V TGCA 6 cut(s) 303, 338, 530, 656, 976, 1030
HpyF10VI GCNNNNNNNGC 6 cut(s) 344, 374, 645, 662, 964, 973
HpyF3I CTNAG 2 cut(s) 195, 387
Hsp92II CATG 7 cut(s) 116, 464, 490, 596, 614, 841, 1051
KspAI GTTAAC 1 cut(s) 118
Kzo9I GATC 6 cut(s) 226, 274, 567, 755, 820, 948
LmnI GCTCC 1 cut(s) 175
Lsp1109I GCAGC 7 cut(s) 280, 377, 455, 461, 542, 657, 988
LweI GCATC 3 cut(s) 24, 665, 1017
MaeI CTAG 5 cut(s) 152, 447, 587, 738, 813
MalI GATC 6 cut(s) 228, 276, 569, 757, 822, 950
MbiI CCGCTC 2 cut(s) 12, 495
MboI GATC 6 cut(s) 226, 274, 567, 755, 820, 948
MfeI CAATTG 1 cut(s) 333
MflI RGATCY 4 cut(s) 226, 274, 820, 948
MluCI AATT 5 cut(s) 333, 393, 550, 604, 992
MlyI GAGTC 2 cut(s) 65, 815
Mph1103I ATGCAT 1 cut(s) 658
MseI TTAA 3 cut(s) 117, 128, 618
MslI CAYNNNNRTG 2 cut(s) 111, 836
MspA1I CMGCKG 1 cut(s) 293
MspI CCGG 5 cut(s) 380, 512, 649, 753, 861
MspR9I CCNGG 4 cut(s) 512, 513, 768, 862
MunI CAATTG 1 cut(s) 333
Mva1269I GAATGC 2 cut(s) 431, 658
MvaI CCWGG 1 cut(s) 768
MwoI GCNNNNNNNGC 6 cut(s) 344, 374, 645, 662, 964, 973
NciI CCSGG 3 cut(s) 512, 513, 862
NdeII GATC 6 cut(s) 226, 274, 567, 755, 820, 948
NheI GCTAGC 1 cut(s) 446
NlaIII CATG 7 cut(s) 116, 464, 490, 596, 614, 841, 1051
NlaIV GGNNCC 4 cut(s) 171, 378, 384, 950
NmeAIII GCCGAG 3 cut(s) 368, 516, 829
NsiI ATGCAT 1 cut(s) 658
NspI RCATGY 3 cut(s) 464, 596, 841
NspV TTCGAA 2 cut(s) 404, 869
PciI ACATGT 2 cut(s) 592, 837
PctI GAATGC 2 cut(s) 431, 658
PfeI GAWTC 3 cut(s) 205, 401, 436
PleI GAGTC 2 cut(s) 65, 814
PpsI GAGTC 2 cut(s) 65, 814
PscI ACATGT 2 cut(s) 592, 837
PshBI ATTAAT 1 cut(s) 128
Psp6I CCWGG 1 cut(s) 766
PspFI CCCAGC 1 cut(s) 730
PspGI CCWGG 1 cut(s) 766
PspN4I GGNNCC 4 cut(s) 171, 378, 384, 950
PstNI CAGNNNCTG 2 cut(s) 296, 668
PsuI RGATCY 4 cut(s) 226, 274, 820, 948
PvuII CAGCTG 1 cut(s) 293
RsaI GTAC 5 cut(s) 465, 591, 597, 719, 842
RsaNI GTAC 5 cut(s) 464, 590, 596, 718, 841
RseI CAYNNNNRTG 2 cut(s) 111, 836
SaqAI TTAA 3 cut(s) 117, 128, 618
Sau3AI GATC 6 cut(s) 226, 274, 567, 755, 820, 948
SchI GAGTC 2 cut(s) 65, 815
ScrFI CCNGG 4 cut(s) 512, 513, 768, 862
SfaNI GCATC 3 cut(s) 24, 665, 1017
SfuI TTCGAA 2 cut(s) 404, 869
SmaI CCCGGG 1 cut(s) 513
SmiMI CAYNNNNRTG 2 cut(s) 111, 836
SpeI ACTAGT 2 cut(s) 586, 737
Sse9I AATT 5 cut(s) 333, 393, 550, 604, 992
SsiI CCGC 7 cut(s) 12, 324, 477, 495, 792, 800, 877
SspMI CTAG 5 cut(s) 152, 447, 587, 738, 813
StyD4I CCNGG 4 cut(s) 510, 511, 766, 860
TaaI ACNGT 1 cut(s) 1065
TaqI TCGA 5 cut(s) 203, 404, 421, 570, 869
TasI AATT 5 cut(s) 333, 393, 550, 604, 992
TatI WGTACW 2 cut(s) 463, 589
TauI GCSGC 3 cut(s) 15, 327, 803
TfiI GAWTC 3 cut(s) 205, 401, 436
Tru1I TTAA 3 cut(s) 117, 128, 618
Tru9I TTAA 3 cut(s) 117, 128, 618
TscAI CASTG 1 cut(s) 457
TseI GCWGC 7 cut(s) 293, 365, 443, 474, 530, 645, 976
TspDTI ATGAA 5 cut(s) 87, 95, 475, 692, 1064
TspGWI ACGGA 1 cut(s) 736
TspMI CCCGGG 1 cut(s) 511
TspRI CASTG 1 cut(s) 457
VspI ATTAAT 1 cut(s) 128
XapI RAATTY 1 cut(s) 550
XceI RCATGY 3 cut(s) 464, 596, 841
XmaI CCCGGG 1 cut(s) 511
XspI CTAG 5 cut(s) 152, 447, 587, 738, 813
Zsp2I ATGCAT 1 cut(s) 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.