MD15G1264400.v1.1

U1 small nuclear ribonucleoprotein 70

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009950
Physical Location & Seq
Reverse (-)
22655808 .. 22657266
1459 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1264400.v1.1.491

Sequence Viewer

Length: 540 bp
ATGGGAGACTTCAACAACGATGCTTTCATGCGCGGCCAAAACGCCGCCGTTCAGGGCCGAACCAAGGCCCAGAACCGCCAAAATGTTCTTCAGCTCAAGATGATTGGGCAGAGCCACCCCACCGGTCTAACAACAAACCTTTTGAAGCTCTTTGAGCCTAGACTGCCGTTGGATTTCAAACCGCCTCCAGAGAAGAAAAAGTGCCCACCTTTGACAGGGGTAGCGCAATTTGTGAGTAAGTTTGCGGAGCCCGGAGATCCTGAGTATAATCCGCCTGTCCAAAAGGGCGAAACTCCTACACAGAGAAGGGCTCGAATACACCAGCTAAGACTTGAGAAGGGTGCAGCAAAGGCTGCGGAAGAGCTAGAGAAATATGATCCAAATAATGACCCAAGTATTTCTGGAGATCCATACAAGACTTTGTTTGTTGCAAGACTCAATTACGAGACAACTGAGAGCAGAATCAAAAGGGAGTTTGACGTTTATGGTCCAATCAAGCGGGTAGGTATTTTTTTGAGAAACCCCAAGGGGGTTTGGTAA

Protein Analysis

180

Amino Acids

20.26

Weight (kDa)

9.71

Isoelectric Point (pI)

33.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U1snRNP70_N PF12220 42 - 130 8.5e-20 U1 small nuclear ribonucleoprotein of 70kDa MW N terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 33
AciI CCGC 8 cut(s) 33, 45, 76, 182, 245, 272, 356, 499
AclWI GGATC 3 cut(s) 251, 371, 401
AcoI YGGCCR 1 cut(s) 34
AcuI CTGAAG 1 cut(s) 74
AfiI CCNNNNNNNGG 3 cut(s) 64, 215, 529
AgeI ACCGGT 1 cut(s) 122
AgsI TTSAA 3 cut(s) 13, 145, 178
AjuI GAANNNNNNNTTGG 2 cut(s) 72, 104
AluBI AGCT 4 cut(s) 94, 148, 325, 364
AluI AGCT 4 cut(s) 94, 148, 325, 364
Alw26I GTCTC 1 cut(s) 440
AlwI GGATC 3 cut(s) 251, 371, 401
AoxI GGCC 3 cut(s) 34, 55, 66
ApeKI GCWGC 2 cut(s) 344, 353
AsiGI ACCGGT 1 cut(s) 122
AspLEI GCGC 2 cut(s) 33, 226
AspS9I GGNCC 3 cut(s) 55, 67, 488
AsuC2I CCSGG 1 cut(s) 252
AvaII GGWCC 1 cut(s) 488
BaeGI GKGCMC 1 cut(s) 206
BanII GRGCYC 2 cut(s) 252, 313
BbvI GCAGC 2 cut(s) 340, 356
BceAI ACGGC 2 cut(s) 32, 151
BcnI CCSGG 1 cut(s) 252
BcoDI GTCTC 1 cut(s) 440
BfaI CTAG 2 cut(s) 159, 365
BisI GCNGC 4 cut(s) 34, 45, 345, 354
BlsI GCNGC 4 cut(s) 35, 46, 346, 355
Bme1390I CCNGG 1 cut(s) 252
Bme18I GGWCC 1 cut(s) 488
BmgT120I GGNCC 3 cut(s) 55, 67, 488
BmiI GGNNCC 1 cut(s) 249
BmrFI CCNGG 1 cut(s) 252
BmsI GCATC 1 cut(s) 10
BplI GAGNNNNNCTC 2 cut(s) 295, 327
BpmI CTGGAG 2 cut(s) 171, 423
BpuEI CTTGAG 2 cut(s) 80, 353
BpuMI CCSGG 1 cut(s) 252
BsaJI CCNNGG 2 cut(s) 63, 525
BsaWI WCCGGW 1 cut(s) 122
Bsc4I CCNNNNNNNGG 3 cut(s) 64, 215, 529
Bse118I RCCGGY 1 cut(s) 122
BseDI CCNNGG 2 cut(s) 63, 525
BseLI CCNNNNNNNGG 3 cut(s) 64, 215, 529
BseMII CTCAG 2 cut(s) 252, 444
BseSI GKGCMC 1 cut(s) 206
BseXI GCAGC 2 cut(s) 340, 356
BsgI GTGCAG 1 cut(s) 363
Bsh1236I CGCG 1 cut(s) 33
BshFI GGCC 3 cut(s) 36, 57, 68
BshTI ACCGGT 1 cut(s) 122
BsiSI CCGG 2 cut(s) 123, 252
BslI CCNNNNNNNGG 3 cut(s) 64, 215, 529
BsmAI GTCTC 1 cut(s) 440
BsnI GGCC 3 cut(s) 36, 57, 68
Bsp1286I GDGCHC 3 cut(s) 206, 252, 313
Bsp143I GATC 3 cut(s) 256, 376, 406
BspACI CCGC 8 cut(s) 33, 45, 76, 182, 245, 272, 356, 499
BspANI GGCC 3 cut(s) 36, 57, 68
BspCNI CTCAG 2 cut(s) 253, 445
BspFNI CGCG 1 cut(s) 33
BspLI GGNNCC 1 cut(s) 249
BspPI GGATC 3 cut(s) 251, 371, 401
BspQI GCTCTTC 1 cut(s) 354
BsrFI RCCGGY 1 cut(s) 122
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 2 cut(s) 63, 525
BssMI GATC 3 cut(s) 256, 376, 406
BssT1I CCWWGG 2 cut(s) 63, 525
Bst6I CTCTTC 1 cut(s) 354
BstAPI GCANNNNNTGC 1 cut(s) 353
BstDEI CTNAG 3 cut(s) 261, 326, 453
BstENI CCTNNNNNAGG 1 cut(s) 213
BstFNI CGCG 1 cut(s) 33
BstHHI GCGC 2 cut(s) 33, 226
BstKTI GATC 3 cut(s) 259, 379, 409
BstMAI GTCTC 1 cut(s) 440
BstMBI GATC 3 cut(s) 256, 376, 406
BstMWI GCNNNNNNNGC 4 cut(s) 154, 163, 350, 353
BstSCI CCNGG 1 cut(s) 250
BstSLI GKGCMC 1 cut(s) 206
BstUI CGCG 1 cut(s) 33
BstV1I GCAGC 2 cut(s) 340, 356
BstX2I RGATCY 2 cut(s) 256, 406
BstYI RGATCY 2 cut(s) 256, 406
BsuRI GGCC 3 cut(s) 36, 57, 68
CfoI GCGC 2 cut(s) 33, 226
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 3 cut(s) 55, 67, 488
CspAI ACCGGT 1 cut(s) 122
CviAII CATG 1 cut(s) 28
DdeI CTNAG 3 cut(s) 261, 326, 453
DpnI GATC 3 cut(s) 258, 378, 408
DpnII GATC 3 cut(s) 256, 376, 406
EaeI YGGCCR 1 cut(s) 34
Eam1104I CTCTTC 1 cut(s) 354
EarI CTCTTC 1 cut(s) 354
EciI GGCGGA 1 cut(s) 261
Eco130I CCWWGG 2 cut(s) 63, 525
Eco24I GRGCYC 2 cut(s) 252, 313
Eco47I GGWCC 1 cut(s) 488
Eco57I CTGAAG 1 cut(s) 74
EcoNI CCTNNNNNAGG 1 cut(s) 213
EcoT14I CCWWGG 2 cut(s) 63, 525
EcoT38I GRGCYC 2 cut(s) 252, 313
ErhI CCWWGG 2 cut(s) 63, 525
FaeI CATG 1 cut(s) 31
FaiI YATR 5 cut(s) 29, 267, 375, 412, 486
FatI CATG 1 cut(s) 27
FauI CCCGC 1 cut(s) 492
Fnu4HI GCNGC 4 cut(s) 34, 45, 345, 354
FriOI GRGCYC 2 cut(s) 252, 313
Fsp4HI GCNGC 4 cut(s) 34, 45, 345, 354
FspBI CTAG 2 cut(s) 159, 365
GlaI GCGC 2 cut(s) 32, 225
GluI GCNGC 4 cut(s) 34, 45, 345, 354
GsuI CTGGAG 2 cut(s) 171, 423
HaeIII GGCC 3 cut(s) 36, 57, 68
HapII CCGG 2 cut(s) 123, 252
HhaI GCGC 2 cut(s) 33, 226
Hin1II CATG 1 cut(s) 31
Hin6I GCGC 2 cut(s) 31, 224
HinP1I GCGC 2 cut(s) 31, 224
HinfI GANTC 2 cut(s) 435, 462
HpaII CCGG 2 cut(s) 123, 252
Hpy188III TCNNGA 4 cut(s) 97, 188, 260, 402
HpyAV CCTTC 2 cut(s) 300, 331
HpyCH4IV ACGT 1 cut(s) 480
HpyCH4V TGCA 2 cut(s) 344, 431
HpyF10VI GCNNNNNNNGC 4 cut(s) 154, 163, 350, 353
HpyF3I CTNAG 3 cut(s) 261, 326, 453
HpySE526I ACGT 1 cut(s) 480
Hsp92II CATG 1 cut(s) 31
HspAI GCGC 2 cut(s) 31, 224
Kzo9I GATC 3 cut(s) 256, 376, 406
LguI GCTCTTC 1 cut(s) 354
LmnI GCTCC 1 cut(s) 247
Lsp1109I GCAGC 2 cut(s) 340, 356
LweI GCATC 1 cut(s) 10
MaeI CTAG 2 cut(s) 159, 365
MaeII ACGT 1 cut(s) 480
MalI GATC 3 cut(s) 258, 378, 408
MboI GATC 3 cut(s) 256, 376, 406
MboII GAAGA 3 cut(s) 80, 205, 371
MflI RGATCY 2 cut(s) 256, 406
MhlI GDGCHC 3 cut(s) 206, 252, 313
MluCI AATT 2 cut(s) 227, 439
MlyI GAGTC 1 cut(s) 429
MmeI TCCRAC 1 cut(s) 150
MnlI CCTC 1 cut(s) 195
MspI CCGG 2 cut(s) 123, 252
MspR9I CCNGG 1 cut(s) 252
MvnI CGCG 1 cut(s) 33
MwoI GCNNNNNNNGC 4 cut(s) 154, 163, 350, 353
NciI CCSGG 1 cut(s) 252
NdeII GATC 3 cut(s) 256, 376, 406
NlaIII CATG 1 cut(s) 31
NlaIV GGNNCC 1 cut(s) 249
PciSI GCTCTTC 1 cut(s) 354
PfeI GAWTC 1 cut(s) 462
PinAI ACCGGT 1 cut(s) 122
PkrI GCNGC 4 cut(s) 35, 46, 346, 355
PleI GAGTC 1 cut(s) 429
PpsI GAGTC 1 cut(s) 429
PspN4I GGNNCC 1 cut(s) 249
PspPI GGNCC 3 cut(s) 55, 67, 488
PsuI RGATCY 2 cut(s) 256, 406
SapI GCTCTTC 1 cut(s) 354
SatI GCNGC 4 cut(s) 34, 45, 345, 354
Sau3AI GATC 3 cut(s) 256, 376, 406
Sau96I GGNCC 3 cut(s) 55, 67, 488
SchI GAGTC 1 cut(s) 429
ScrFI CCNGG 1 cut(s) 252
SduI GDGCHC 3 cut(s) 206, 252, 313
SetI ASST 8 cut(s) 96, 141, 150, 211, 327, 366, 483, 508
SfaNI GCATC 1 cut(s) 10
SinI GGWCC 1 cut(s) 488
SmlI CTYRAG 2 cut(s) 95, 332
SmoI CTYRAG 2 cut(s) 95, 332
Sse9I AATT 2 cut(s) 227, 439
SsiI CCGC 8 cut(s) 33, 45, 76, 182, 245, 272, 356, 499
SspMI CTAG 2 cut(s) 159, 365
StyD4I CCNGG 1 cut(s) 250
StyI CCWWGG 2 cut(s) 63, 525
TaiI ACGT 1 cut(s) 483
TaqI TCGA 1 cut(s) 313
TasI AATT 2 cut(s) 227, 439
TauI GCSGC 2 cut(s) 36, 47
TfiI GAWTC 1 cut(s) 462
TseI GCWGC 2 cut(s) 344, 353
TspDTI ATGAA 1 cut(s) 16
VpaK11BI GGWCC 1 cut(s) 488
XagI CCTNNNNNAGG 1 cut(s) 213
XspI CTAG 2 cut(s) 159, 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.