MD15G1266400.v1.1

Cupin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012303
Physical Location & Seq
Reverse (-)
22882356 .. 22885095
2740 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1266400.v1.1.491

Sequence Viewer

Length: 1239 bp
ATGTTGAAAAAAGCTCACATCTTCTTCTTCTTCCTGCTTTCTGTTGCTGTGTCTTTCCATGCAGCAAATGCACTGAAAAATGAATGTGAATGTGGTTCAGACGGTGGCGGTGGTGCTCCGGCTGGGTCTCTGGTGAATATAAATCAACGGCGTTCACTGGTTTCAACTGAGTATGGAGAGGTCTGGTCCGTTGATGTGAATGATGGACACGGGAGGCCTTACCATATCCAGTTCATCACTTTGGAACCCGACTCGCTCTTCCTTCCGGTGCTTCTCCATGCAGACATGGTTTTCTATGTCAACACAGGGAGTGGGAGGTTGAGTTGGACTGATGATGATGAAATTAGAAGGGTGGATATAAAGAGAGGTGATCTCTATAGACTCTACCCAGGCACTGTTTTCTATGTACAAAGTAACTTAGAGACTGAGCGCCCGAAGCTGAGAATTAATGCAATCTTTGCAAGCAACTCTGATGATGACTTATATGATCCAGCAGTAGGGGCATACTCCAGCATTAGGGACATAGTCCGAGGGTTTGATCCGAAATTGCTTCGGTCAGCCTTCAAGGTCTCTGAGGAAGTGATAGAATCAATAGTAAATGGAACAGACCAATCTGCCATTGTGCATGCAATTCCAACAAAGAAAGAAACTTTCTGGGATTTGGAGGCTCGATTCCTGAAAACGTTTCTACTAGGCAATGATGGCATTGCATTCAACAGTAAGAAGAAGACAACAACGTATAATATTTTCGACGAGGAACCAGATTTCGAGAATTGCAACGGTTGGAGCCTAACTGTGAACAAAAAAACCTCCAACTTGTTAAAGGGATCAAACATTGGTCTTTTCATGGTGAATTTGACAAAGGGGTCAATGATGGGACCTCATTGGAATCCAAGGGCAACTGAGATAGCAATAGTACTACAAGGGCAAGGGATGGTTAGGGTGGTTTGTTCAAGCACCGCAGTGAAGAAATCGGAATGCAGAAACATGCGGTTTAGGGTTCATGAAGGAGATGTATTTGCTGTGCCTAGGTTCCATCCAATGGCTCAGATGTCATTTAACAATAACTCTCTTGTGTTCATGGGGTTCAGCACGACAGAAAAGAAAAACAAACCTCAGTTTTTGGCTGGAAAATACTCGGTTCTGCAGACTTTGGACAAACAGGTCTTGGCAGCATCGTTTAATGTTACCAACACAACGATTGATCAACTTCTGTCTGTGCAGCCCGAGTCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0043207 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

413

Amino Acids

46.06

Weight (kDa)

7.12

Isoelectric Point (pI)

39.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 247 - 401 1.3e-40 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012303)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G36700
fragaria_vesca FvH4_1g13730 FvH4_1g13730 FvH4_1g13730
malus_domestica MD02G1152000.v1.1 MD15G1266400.v1.1
prunus_persica Prupe.7G151000_v2.0.a1 Prupe.7G151000_v2.0.a1
pyrus_communis pycom02g11940
rosa_chinensis RchiOBHm_Chr2g0102141
rosa_laevigata RLG00000017133
rosa_roxburghii Rroxscaffold_2G00140750
rosa_rugosa Rorug02G0104600 Rorug02G0104700
rosa_samantha Rh2AG153100 Rh2BG159000 Rh2CG158300 Rh2DG158700
rosa_wichuraiana Rw2G011950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 865, 1163
AccB7I CCANNNNNTGG 1 cut(s) 1042
AciI CCGC 3 cut(s) 108, 960, 991
AclI AACGTT 1 cut(s) 683
AclWI GGATC 3 cut(s) 482, 533, 835
AcsI RAATTY 1 cut(s) 853
AfaI GTAC 2 cut(s) 408, 918
AfiI CCNNNNNNNGG 3 cut(s) 497, 516, 1042
AgsI TTSAA 5 cut(s) 7, 165, 565, 715, 954
AjnI CCWGG 1 cut(s) 388
AleI CACNNNNGTG 1 cut(s) 962
AluBI AGCT 2 cut(s) 14, 439
AluI AGCT 2 cut(s) 14, 439
Alw21I GWGCWC 1 cut(s) 118
Alw26I GTCTC 3 cut(s) 132, 416, 574
AlwI GGATC 3 cut(s) 482, 533, 835
AlwNI CAGNNNCTG 1 cut(s) 395
Ama87I CYCGRG 1 cut(s) 1226
AoxI GGCC 1 cut(s) 215
ApeKI GCWGC 3 cut(s) 62, 1172, 1222
ApoI RAATTY 1 cut(s) 853
AseI ATTAAT 1 cut(s) 447
AspA2I CCTAGG 1 cut(s) 1028
AspLEI GCGC 1 cut(s) 432
AspS9I GGNCC 2 cut(s) 186, 878
AsuHPI GGTGA 3 cut(s) 145, 380, 862
AvaI CYCGRG 1 cut(s) 1226
AvaII GGWCC 2 cut(s) 186, 878
AvrII CCTAGG 1 cut(s) 1028
BbsI GAAGAC 1 cut(s) 734
Bbv12I GWGCWC 1 cut(s) 118
BbvI GCAGC 3 cut(s) 74, 1184, 1234
BccI CCATC 5 cut(s) 197, 695, 868, 928, 1044
BceAI ACGGC 1 cut(s) 164
BciT130I CCWGG 1 cut(s) 390
BclI TGATCA 1 cut(s) 1204
BcoDI GTCTC 3 cut(s) 132, 416, 574
BfaI CTAG 2 cut(s) 692, 1029
BfmI CTRYAG 2 cut(s) 376, 1145
BfoI RGCGCY 1 cut(s) 433
BisI GCNGC 3 cut(s) 63, 1173, 1223
BlnI CCTAGG 1 cut(s) 1028
BlsI GCNGC 3 cut(s) 64, 1174, 1224
BmcAI AGTACT 1 cut(s) 918
Bme1390I CCNGG 1 cut(s) 390
Bme18I GGWCC 2 cut(s) 186, 878
BmeT110I CYCGRG 1 cut(s) 1226
BmgT120I GGNCC 2 cut(s) 186, 878
BmiI GGNNCC 5 cut(s) 246, 759, 788, 879, 1034
BmrFI CCNGG 1 cut(s) 390
BmsI GCATC 1 cut(s) 1184
BpiI GAAGAC 1 cut(s) 734
BplI GAGNNNNNCTC 2 cut(s) 357, 389
BpmI CTGGAG 1 cut(s) 493
BsaI GGTCTC 2 cut(s) 132, 574
BsaJI CCNNGG 4 cut(s) 388, 529, 893, 1028
BsaWI WCCGGW 1 cut(s) 265
BsaXI ACNNNNNCTCC 2 cut(s) 307, 337
Bsc4I CCNNNNNNNGG 3 cut(s) 497, 516, 1042
Bse1I ACTGG 2 cut(s) 162, 229
Bse3DI GCAATG 2 cut(s) 703, 705
BseBI CCWGG 1 cut(s) 390
BseDI CCNNGG 4 cut(s) 388, 529, 893, 1028
BseGI GGATG 2 cut(s) 939, 1036
BseLI CCNNNNNNNGG 3 cut(s) 497, 516, 1042
BseMI GCAATG 2 cut(s) 703, 705
BseMII CTCAG 7 cut(s) 159, 417, 431, 564, 894, 1061, 1130
BseNI ACTGG 2 cut(s) 162, 229
BseXI GCAGC 3 cut(s) 74, 1184, 1234
BseYI CCCAGC 1 cut(s) 122
BshFI GGCC 1 cut(s) 217
BsiHKAI GWGCWC 1 cut(s) 118
BsiHKCI CYCGRG 1 cut(s) 1226
BsiSI CCGG 2 cut(s) 119, 266
BslFI GGGAC 2 cut(s) 533, 891
BslI CCNNNNNNNGG 3 cut(s) 497, 516, 1042
BsmAI GTCTC 3 cut(s) 132, 416, 574
BsmFI GGGAC 2 cut(s) 533, 891
BsmI GAATGC 2 cut(s) 710, 983
BsnI GGCC 1 cut(s) 217
Bso31I GGTCTC 2 cut(s) 132, 574
BsoBI CYCGRG 1 cut(s) 1226
Bsp1286I GDGCHC 1 cut(s) 118
Bsp1407I TGTACA 1 cut(s) 406
Bsp143I GATC 5 cut(s) 370, 487, 538, 827, 1204
BspACI CCGC 3 cut(s) 108, 960, 991
BspANI GGCC 1 cut(s) 217
BspCNI CTCAG 7 cut(s) 160, 418, 432, 565, 895, 1060, 1129
BspHI TCATGA 1 cut(s) 1003
BspLI GGNNCC 5 cut(s) 246, 759, 788, 879, 1034
BspMAI CTGCAG 1 cut(s) 1149
BspPI GGATC 3 cut(s) 482, 533, 835
BspQI GCTCTTC 1 cut(s) 263
BspTNI GGTCTC 2 cut(s) 132, 574
BsrDI GCAATG 2 cut(s) 703, 705
BsrGI TGTACA 1 cut(s) 406
BsrI ACTGG 2 cut(s) 162, 229
BssECI CCNNGG 4 cut(s) 388, 529, 893, 1028
BssMI GATC 5 cut(s) 370, 487, 538, 827, 1204
BssT1I CCWWGG 2 cut(s) 893, 1028
Bst2UI CCWGG 1 cut(s) 390
Bst4CI ACNGT 5 cut(s) 104, 397, 719, 782, 796
Bst6I CTCTTC 1 cut(s) 263
BstAPI GCANNNNNTGC 2 cut(s) 68, 458
BstAUI TGTACA 1 cut(s) 406
BstC8I GCNNGC 2 cut(s) 463, 627
BstDEI CTNAG 8 cut(s) 168, 418, 426, 440, 573, 903, 1047, 1116
BstF5I GGATG 2 cut(s) 939, 1036
BstH2I RGCGCY 1 cut(s) 433
BstHHI GCGC 1 cut(s) 432
BstKTI GATC 5 cut(s) 373, 490, 541, 830, 1207
BstMAI GTCTC 3 cut(s) 132, 416, 574
BstMBI GATC 5 cut(s) 370, 487, 538, 827, 1204
BstMWI GCNNNNNNNGC 5 cut(s) 68, 436, 458, 500, 702
BstNI CCWGG 1 cut(s) 390
BstNSI RCATGY 2 cut(s) 629, 991
BstSCI CCNGG 1 cut(s) 388
BstSFI CTRYAG 2 cut(s) 376, 1145
BstV1I GCAGC 3 cut(s) 74, 1184, 1234
BstV2I GAAGAC 1 cut(s) 734
BsuRI GGCC 1 cut(s) 217
BtsCI GGATG 2 cut(s) 939, 1036
BtsI GCAGTG 1 cut(s) 969
BtsIMutI CAGTG 4 cut(s) 71, 155, 393, 969
Cac8I GCNNGC 2 cut(s) 463, 627
CaiI CAGNNNCTG 1 cut(s) 395
CciI TCATGA 1 cut(s) 1003
CfoI GCGC 1 cut(s) 432
Cfr13I GGNCC 2 cut(s) 186, 878
Csp6I GTAC 2 cut(s) 407, 917
CviAII CATG 8 cut(s) 59, 278, 286, 626, 847, 988, 1004, 1081
CviQI GTAC 2 cut(s) 407, 917
DdeI CTNAG 8 cut(s) 168, 418, 426, 440, 573, 903, 1047, 1116
DpnI GATC 5 cut(s) 372, 489, 540, 829, 1206
DpnII GATC 5 cut(s) 370, 487, 538, 827, 1204
DrdI GACNNNNNNGTC 2 cut(s) 865, 1163
DseDI GACNNNNNNGTC 2 cut(s) 865, 1163
Eam1104I CTCTTC 1 cut(s) 263
EarI CTCTTC 1 cut(s) 263
Eco130I CCWWGG 2 cut(s) 893, 1028
Eco147I AGGCCT 1 cut(s) 217
Eco31I GGTCTC 2 cut(s) 132, 574
Eco47I GGWCC 2 cut(s) 186, 878
Eco88I CYCGRG 1 cut(s) 1226
EcoO109I RGGNCCY 1 cut(s) 878
EcoRII CCWGG 1 cut(s) 388
EcoT14I CCWWGG 2 cut(s) 893, 1028
ErhI CCWWGG 2 cut(s) 893, 1028
FaeI CATG 8 cut(s) 62, 281, 289, 629, 850, 991, 1007, 1084
FaqI GGGAC 2 cut(s) 533, 891
FatI CATG 8 cut(s) 58, 277, 285, 625, 846, 987, 1003, 1080
FbaI TGATCA 1 cut(s) 1204
Fnu4HI GCNGC 3 cut(s) 63, 1173, 1223
FokI GGATG 2 cut(s) 946, 1023
Fsp4HI GCNGC 3 cut(s) 63, 1173, 1223
FspBI CTAG 2 cut(s) 692, 1029
GlaI GCGC 1 cut(s) 431
GluI GCNGC 3 cut(s) 63, 1173, 1223
GsaI CCCAGC 1 cut(s) 126
GsuI CTGGAG 1 cut(s) 493
HaeII RGCGCY 1 cut(s) 433
HaeIII GGCC 1 cut(s) 217
HapII CCGG 2 cut(s) 119, 266
HhaI GCGC 1 cut(s) 432
Hin1II CATG 8 cut(s) 62, 281, 289, 629, 850, 991, 1007, 1084
Hin6I GCGC 1 cut(s) 430
HinP1I GCGC 1 cut(s) 430
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HinfI GANTC 6 cut(s) 251, 381, 587, 672, 889, 1229
HpaII CCGG 2 cut(s) 119, 266
HphI GGTGA 3 cut(s) 145, 380, 862
Hpy166II GTNNAC 3 cut(s) 155, 301, 799
Hpy188I TCNGA 7 cut(s) 100, 472, 530, 543, 574, 976, 1050
Hpy188III TCNNGA 3 cut(s) 676, 769, 1004
Hpy8I GTNNAC 3 cut(s) 155, 301, 799
Hpy99I CGWCG 1 cut(s) 755
HpyAV CCTTC 4 cut(s) 272, 342, 571, 1001
HpyCH4III ACNGT 5 cut(s) 104, 397, 719, 782, 796
HpyCH4IV ACGT 2 cut(s) 683, 737
HpyF10VI GCNNNNNNNGC 5 cut(s) 68, 436, 458, 500, 702
HpyF3I CTNAG 8 cut(s) 168, 418, 426, 440, 573, 903, 1047, 1116
HpySE526I ACGT 2 cut(s) 683, 737
Hsp92II CATG 8 cut(s) 62, 281, 289, 629, 850, 991, 1007, 1084
HspAI GCGC 1 cut(s) 430
Ksp22I TGATCA 1 cut(s) 1204
Kzo9I GATC 5 cut(s) 370, 487, 538, 827, 1204
LguI GCTCTTC 1 cut(s) 263
LmnI GCTCC 2 cut(s) 121, 786
Lsp1109I GCAGC 3 cut(s) 74, 1184, 1234
LweI GCATC 1 cut(s) 1184
MaeI CTAG 2 cut(s) 692, 1029
MaeII ACGT 2 cut(s) 683, 737
MaeIII GTNAC 2 cut(s) 413, 1186
MalI GATC 5 cut(s) 372, 489, 540, 829, 1206
MboI GATC 5 cut(s) 370, 487, 538, 827, 1204
MboII GAAGA 8 cut(s) 13, 16, 19, 22, 250, 736, 739, 979
MhlI GDGCHC 1 cut(s) 118
MluCI AATT 6 cut(s) 342, 444, 545, 630, 772, 853
MlyI GAGTC 3 cut(s) 245, 375, 1238
MmeI TCCRAC 4 cut(s) 305, 659, 764, 837
MseI TTAA 4 cut(s) 447, 821, 1059, 1182
MslI CAYNNNNRTG 1 cut(s) 962
MspI CCGG 2 cut(s) 119, 266
MspR9I CCNGG 1 cut(s) 390
Mva1269I GAATGC 2 cut(s) 710, 983
MvaI CCWGG 1 cut(s) 390
MwoI GCNNNNNNNGC 5 cut(s) 68, 436, 458, 500, 702
NdeII GATC 5 cut(s) 370, 487, 538, 827, 1204
NlaIII CATG 8 cut(s) 62, 281, 289, 629, 850, 991, 1007, 1084
NlaIV GGNNCC 5 cut(s) 246, 759, 788, 879, 1034
NspI RCATGY 2 cut(s) 629, 991
OliI CACNNNNGTG 1 cut(s) 962
PaeI GCATGC 1 cut(s) 629
PagI TCATGA 1 cut(s) 1003
PceI AGGCCT 1 cut(s) 217
PciSI GCTCTTC 1 cut(s) 263
PctI GAATGC 2 cut(s) 710, 983
PfeI GAWTC 3 cut(s) 587, 672, 889
PflFI GACNNNGTC 1 cut(s) 524
PflMI CCANNNNNTGG 1 cut(s) 1042
PkrI GCNGC 3 cut(s) 64, 1174, 1224
PleI GAGTC 3 cut(s) 245, 375, 1237
PpsI GAGTC 3 cut(s) 245, 375, 1237
PpuMI RGGWCCY 1 cut(s) 878
PshBI ATTAAT 1 cut(s) 447
Psp1406I AACGTT 1 cut(s) 683
Psp5II RGGWCCY 1 cut(s) 878
Psp6I CCWGG 1 cut(s) 388
PspFI CCCAGC 1 cut(s) 122
PspGI CCWGG 1 cut(s) 388
PspN4I GGNNCC 5 cut(s) 246, 759, 788, 879, 1034
PspPI GGNCC 2 cut(s) 186, 878
PspPPI RGGWCCY 1 cut(s) 878
PstI CTGCAG 1 cut(s) 1149
PstNI CAGNNNCTG 1 cut(s) 395
PsyI GACNNNGTC 1 cut(s) 524
RsaI GTAC 2 cut(s) 408, 918
RsaNI GTAC 2 cut(s) 407, 917
RseI CAYNNNNRTG 1 cut(s) 962
SapI GCTCTTC 1 cut(s) 263
SaqAI TTAA 4 cut(s) 447, 821, 1059, 1182
SatI GCNGC 3 cut(s) 63, 1173, 1223
Sau3AI GATC 5 cut(s) 370, 487, 538, 827, 1204
Sau96I GGNCC 2 cut(s) 186, 878
ScaI AGTACT 1 cut(s) 918
SchI GAGTC 3 cut(s) 245, 375, 1238
ScrFI CCNGG 1 cut(s) 390
SduI GDGCHC 1 cut(s) 118
SfaNI GCATC 1 cut(s) 1184
SfcI CTRYAG 2 cut(s) 376, 1145
SinI GGWCC 2 cut(s) 186, 878
SmiMI CAYNNNNRTG 1 cut(s) 962
SphI GCATGC 1 cut(s) 629
Sse9I AATT 6 cut(s) 342, 444, 545, 630, 772, 853
SseBI AGGCCT 1 cut(s) 217
SsiI CCGC 3 cut(s) 108, 960, 991
SspI AATATT 1 cut(s) 745
SspMI CTAG 2 cut(s) 692, 1029
StuI AGGCCT 1 cut(s) 217
StyD4I CCNGG 1 cut(s) 388
StyI CCWWGG 2 cut(s) 893, 1028
TaaI ACNGT 5 cut(s) 104, 397, 719, 782, 796
TaiI ACGT 2 cut(s) 686, 740
TaqI TCGA 3 cut(s) 670, 750, 768
TaqII GACCGA 1 cut(s) 543
TasI AATT 6 cut(s) 342, 444, 545, 630, 772, 853
TatI WGTACW 2 cut(s) 406, 916
TfiI GAWTC 3 cut(s) 587, 672, 889
Tru1I TTAA 4 cut(s) 447, 821, 1059, 1182
Tru9I TTAA 4 cut(s) 447, 821, 1059, 1182
TscAI CASTG 4 cut(s) 78, 162, 400, 969
TseI GCWGC 3 cut(s) 62, 1172, 1222
TspDTI ATGAA 7 cut(s) 96, 223, 354, 835, 992, 1020, 1069
TspGWI ACGGA 1 cut(s) 178
TspRI CASTG 4 cut(s) 78, 162, 400, 969
Tth111I GACNNNGTC 1 cut(s) 524
Van91I CCANNNNNTGG 1 cut(s) 1042
VpaK11BI GGWCC 2 cut(s) 186, 878
VspI ATTAAT 1 cut(s) 447
XapI RAATTY 1 cut(s) 853
XceI RCATGY 2 cut(s) 629, 991
XmaJI CCTAGG 1 cut(s) 1028
XspI CTAG 2 cut(s) 692, 1029
ZrmI AGTACT 1 cut(s) 918
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.