Rw2G011950

Cupin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0012303
Physical Location & Seq
Reverse (-)
12127979 .. 12131075
3097 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G011950.1

Sequence Viewer

Length: 1854 bp
ATGTCTAACAACGATTCAAATCCACCGTTTTCACTTACTCTGTTCATCTTATTCTTCTTTCTTTCGCTACCTGCCTTCTCTTCCTCTTTGAAGTCTTTGAAAAATGAGGAGGATTTTTCCAGTAGCGATGCCGGGTATCTGGTGAGGAGAGCTCAGAGGAGGGCGTTGGTTCAGACAGAGTACGGAGAAATCTCGTCCATTGATGTCACCGATGGACGGAGAGGGCCTTACCATGTCCAGTTCATCATCATGGAACCCAACTCGCTGTTCCTTCCGGTGCTGCTACACTCTGACATGGTGTTCTATGTCCACACAGGGAGTGGGAGGTTGAATTGGGCTCATGGAAAGGATGATCAAATCAACGAGGTTCCTTTAAAGCAAGGTGATATCCATAGACTCCGCCCAGGATCTGTCTTCTTTGTACAGAGTGATTTACAGACCGAGCGTCAGAAACTTAGAATTAATGCAATCTTTGCAAATAGCGATGATGACGTATTTGACCCAACAATAGGAGCATACTCCAGTGTTAGGGACCTGGTCCGAGGCTTCGACACCAAAATCCTTCGGTCAGCTTTTCAGGTTTCTGAGGATGTGATCGAGTCGATAACAAATTCAACAGACCAATCAGCCATTATCCATGCAGTGCCAACGAAGAAAGAAACCTTTTGGGATTTGGAAGCTCGGTTCTTGAAAACCTTTATAGGAGCCAGTTATGATGGCATGGCATTAAACAAGAAGAAGCATAAGAAGAAAGAAAAATCAAAAACATATAATATTTTTGATGAGGACCCAGATTTCAAGAATTGTAACGGTTGGAGCCTCACCGTGAATGAGAGAAACTCGCCTTCATTGACTGGATCAAACATTGGTCTTTTCATGGTGAACTTGACGAAGGGTTCAATGATGGGGCCTCATTGGAATCCAAGGGCAACTGAGATCGCAGTTGTGTTACAGGGACAAGGAATGGTCAGGGTGGTATGTGCTAGCACAGCAAAGAGATCAGAATGCAGAAATTTGAGGTTTAGGGTTCATGAAGGAGATGTATTTGCTGTACCTATGTTCCATCCAATGGCTCAGATGTCATATAATAATGACTCATTTGTGTTTATGGGGTTCAGCACGACAACAAGGAGAAATTATCCTCAATTTTTGGCTGGGAAATACTCGGTTCTCCAAACTTTGAACAGACAGGTCTTGGCAGTGTCTTTGAATGTTACCAACACCACAATCGATCAGCTTTTGGCTGCACAAACAGAATCAATCATACTCGACTGTACTTCTTGCGCTGAAAAAGAGGAGAAAGTAATGTTGGAAGAAATTGAAAAGGAAAGGGAGGAAGAGGAGGCTAAGAAGAGAGAGGAACAGAGGAAGAGAGAAGAGGAGCGAAGGAAGAGAGAGGAGGAGGAAGCGAGAAAGAGAGAAGAGGAAGAAGAGAGGAGAAAGAGAGAAGAGGAACAAAGGAAGAGAGAGGAGGAGCAGAGACAGAGAGAGGAGGAGGAAGCGAGAAAGAGAGAAGAGGAAGAAGAGAGGAGAAAGAGAGAAGAGGAAGAAGAAGAAGAGAGGAGAAGGAGAGAAGAAGAAAGAAGAGAGGAGGAAGAACAAGAGAGGGAGAGGCAACAGGAAGAAGAAGGAGAAGCAGCAGAAAGGGAGCAAGAAGAGGCTAGGAGGCAAGAGGAAGAAGCAGAGAGTGAGAAAGAAGACACCAGGAGGCAACAACAAGAGGAGTACAGTAAAAGAAAAGAGAAAGAGGCCCAAACAGAGCAAGAGGAGGCTAGGAAACAACAAGAGGAAATAGAAGGAAGAACCAGACCGGAGGAGCAAGAGACGGAAGGTGACCAACGTTTTGACGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0043207 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

617

Amino Acids

72.49

Weight (kDa)

5.1

Isoelectric Point (pI)

86.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 257 - 410 5.9e-38 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012303)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G36700
fragaria_vesca FvH4_1g13730 FvH4_1g13730 FvH4_1g13730
malus_domestica MD02G1152000.v1.1 MD15G1266400.v1.1
prunus_persica Prupe.7G151000_v2.0.a1 Prupe.7G151000_v2.0.a1
pyrus_communis pycom02g11940
rosa_chinensis RchiOBHm_Chr2g0102141
rosa_laevigata RLG00000017133
rosa_roxburghii Rroxscaffold_2G00140750
rosa_rugosa Rorug02G0104600 Rorug02G0104700
rosa_samantha Rh2AG153100 Rh2BG159000 Rh2CG158300 Rh2DG158700
rosa_wichuraiana Rw2G011950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 79
AccB7I CCANNNNNTGG 1 cut(s) 1069
AciI CCGC 1 cut(s) 400
AclI AACGTT 1 cut(s) 1840
AclWI GGATC 2 cut(s) 415, 865
AcsI RAATTY 2 cut(s) 610, 1012
AfaI GTAC 5 cut(s) 182, 423, 1053, 1276, 1727
AfiI CCNNNNNNNGG 4 cut(s) 216, 509, 528, 1069
AhdI GACNNNNNGTC 1 cut(s) 444
AjnI CCWGG 3 cut(s) 403, 534, 1703
AjuI GAANNNNNNNTTGG 4 cut(s) 251, 283, 1292, 1324
AloI GAACNNNNNNTCC 2 cut(s) 668, 700
AluBI AGCT 4 cut(s) 152, 572, 680, 1237
AluI AGCT 4 cut(s) 152, 572, 680, 1237
Alw21I GWGCWC 1 cut(s) 154
Alw26I GTCTC 2 cut(s) 1474, 1817
AlwI GGATC 2 cut(s) 415, 865
AlwNI CAGNNNCTG 1 cut(s) 410
AoxI GGCC 3 cut(s) 224, 908, 1749
ApeKI GCWGC 3 cut(s) 280, 1244, 1637
ApoI RAATTY 2 cut(s) 610, 1012
ArsI GACNNNNNNTTYG 1 cut(s) 1826
AseI ATTAAT 1 cut(s) 462
AspLEI GCGC 1 cut(s) 1286
AspS9I GGNCC 6 cut(s) 224, 532, 538, 787, 908, 1750
AsuC2I CCSGG 1 cut(s) 133
AsuHPI GGTGA 6 cut(s) 154, 199, 395, 814, 892, 1844
AsuNHI GCTAGC 1 cut(s) 983
AvaII GGWCC 3 cut(s) 532, 538, 787
BanII GRGCYC 2 cut(s) 154, 340
BbsI GAAGAC 2 cut(s) 406, 1704
Bbv12I GWGCWC 1 cut(s) 154
BbvI GCAGC 3 cut(s) 267, 1231, 1649
BccI CCATC 4 cut(s) 206, 710, 898, 1071
BciT130I CCWGG 3 cut(s) 405, 536, 1705
BclI TGATCA 1 cut(s) 352
BcnI CCSGG 1 cut(s) 133
BcoDI GTCTC 2 cut(s) 1474, 1817
BfaI CTAG 3 cut(s) 984, 1662, 1773
BfuAI ACCTGC 1 cut(s) 79
BisI GCNGC 3 cut(s) 281, 1245, 1638
BlsI GCNGC 3 cut(s) 282, 1246, 1639
Bme1390I CCNGG 4 cut(s) 133, 405, 536, 1705
Bme18I GGWCC 3 cut(s) 532, 538, 787
BmeRI GACNNNNNGTC 1 cut(s) 444
BmgT120I GGNCC 6 cut(s) 224, 532, 538, 787, 908, 1750
BmiI GGNNCC 7 cut(s) 255, 369, 533, 706, 789, 818, 909
BmrFI CCNGG 4 cut(s) 133, 405, 536, 1705
BmsI GCATC 1 cut(s) 118
BmtI GCTAGC 1 cut(s) 987
BpiI GAAGAC 2 cut(s) 406, 1704
BplI GAGNNNNNCTC 4 cut(s) 136, 168, 824, 856
BpmI CTGGAG 1 cut(s) 505
BpuMI CCSGG 1 cut(s) 133
Bsa29I ATCGAT 1 cut(s) 1230
BsaBI GATNNNNATC 1 cut(s) 18
BsaJI CCNNGG 3 cut(s) 403, 541, 923
BsaWI WCCGGW 2 cut(s) 274, 1810
Bsc4I CCNNNNNNNGG 4 cut(s) 216, 509, 528, 1069
Bse1I ACTGG 5 cut(s) 120, 238, 522, 708, 859
Bse8I GATNNNNATC 1 cut(s) 18
BseBI CCWGG 3 cut(s) 405, 536, 1705
BseCI ATCGAT 1 cut(s) 1230
BseDI CCNNGG 3 cut(s) 403, 541, 923
BseGI GGATG 3 cut(s) 355, 595, 1063
BseJI GATNNNNATC 1 cut(s) 18
BseLI CCNNNNNNNGG 4 cut(s) 216, 509, 528, 1069
BseMII CTCAG 4 cut(s) 167, 576, 924, 1088
BseNI ACTGG 5 cut(s) 120, 238, 522, 708, 859
BseXI GCAGC 3 cut(s) 267, 1231, 1649
BseYI CCCAGC 1 cut(s) 1154
BsgI GTGCAG 1 cut(s) 1230
BshFI GGCC 3 cut(s) 226, 910, 1751
BshVI ATCGAT 1 cut(s) 1230
BsiHKAI GWGCWC 1 cut(s) 154
BsiSI CCGG 3 cut(s) 132, 275, 1811
BslFI GGGAC 2 cut(s) 545, 969
BslI CCNNNNNNNGG 4 cut(s) 216, 509, 528, 1069
BsmAI GTCTC 2 cut(s) 1474, 1817
BsmBI CGTCTC 1 cut(s) 1817
BsmFI GGGAC 2 cut(s) 545, 969
BsmI GAATGC 1 cut(s) 1010
BsnI GGCC 3 cut(s) 226, 910, 1751
Bsp1286I GDGCHC 2 cut(s) 154, 340
Bsp1407I TGTACA 1 cut(s) 421
Bsp143I GATC 7 cut(s) 352, 407, 594, 857, 936, 998, 1231
BspACI CCGC 1 cut(s) 400
BspANI GGCC 3 cut(s) 226, 910, 1751
BspCNI CTCAG 4 cut(s) 166, 577, 925, 1087
BspDI ATCGAT 1 cut(s) 1230
BspHI TCATGA 1 cut(s) 1030
BspLI GGNNCC 7 cut(s) 255, 369, 533, 706, 789, 818, 909
BspMI ACCTGC 1 cut(s) 79
BspOI GCTAGC 1 cut(s) 987
BspPI GGATC 2 cut(s) 415, 865
BsrGI TGTACA 1 cut(s) 421
BsrI ACTGG 5 cut(s) 120, 238, 522, 708, 859
BssECI CCNNGG 3 cut(s) 403, 541, 923
BssMI GATC 7 cut(s) 352, 407, 594, 857, 936, 998, 1231
BssT1I CCWWGG 1 cut(s) 923
Bst2UI CCWGG 3 cut(s) 405, 536, 1705
Bst4CI ACNGT 6 cut(s) 27, 812, 826, 1274, 1730, 1850
BstAPI GCANNNNNTGC 1 cut(s) 473
BstAUI TGTACA 1 cut(s) 421
BstC8I GCNNGC 1 cut(s) 985
BstDEI CTNAG 6 cut(s) 153, 455, 585, 933, 1074, 1347
BstEII GGTNACC 1 cut(s) 1832
BstF5I GGATG 3 cut(s) 355, 595, 1063
BstHHI GCGC 1 cut(s) 1286
BstKTI GATC 7 cut(s) 355, 410, 597, 860, 939, 1001, 1234
BstMAI GTCTC 2 cut(s) 1474, 1817
BstMBI GATC 7 cut(s) 352, 407, 594, 857, 936, 998, 1231
BstMWI GCNNNNNNNGC 2 cut(s) 473, 989
BstNI CCWGG 3 cut(s) 405, 536, 1705
BstPI GGTNACC 1 cut(s) 1832
BstSCI CCNGG 4 cut(s) 131, 403, 534, 1703
BstV1I GCAGC 3 cut(s) 267, 1231, 1649
BstV2I GAAGAC 2 cut(s) 406, 1704
BstX2I RGATCY 1 cut(s) 407
BstYI RGATCY 1 cut(s) 407
Bsu15I ATCGAT 1 cut(s) 1230
BsuRI GGCC 3 cut(s) 226, 910, 1751
BsuTUI ATCGAT 1 cut(s) 1230
BtgZI GCGATG 2 cut(s) 141, 498
BtsCI GGATG 3 cut(s) 355, 595, 1063
BtsI GCAGTG 2 cut(s) 648, 1206
BtsIMutI CAGTG 3 cut(s) 529, 648, 1206
BveI ACCTGC 1 cut(s) 79
Cac8I GCNNGC 1 cut(s) 985
CaiI CAGNNNCTG 1 cut(s) 410
CciI TCATGA 1 cut(s) 1030
CfoI GCGC 1 cut(s) 1286
Cfr13I GGNCC 6 cut(s) 224, 532, 538, 787, 908, 1750
ClaI ATCGAT 1 cut(s) 1230
CseI GACGC 1 cut(s) 434
CsiI ACCWGGT 1 cut(s) 534
Csp6I GTAC 5 cut(s) 181, 422, 1052, 1275, 1726
CviAII CATG 8 cut(s) 233, 250, 295, 341, 638, 721, 877, 1031
CviQI GTAC 5 cut(s) 181, 422, 1052, 1275, 1726
DdeI CTNAG 6 cut(s) 153, 455, 585, 933, 1074, 1347
DpnI GATC 7 cut(s) 354, 409, 596, 859, 938, 1000, 1233
DpnII GATC 7 cut(s) 352, 407, 594, 857, 936, 998, 1231
DraI TTTAAA 1 cut(s) 375
DriI GACNNNNNGTC 1 cut(s) 444
Eam1105I GACNNNNNGTC 1 cut(s) 444
EciI GGCGGA 1 cut(s) 389
Ecl136II GAGCTC 1 cut(s) 152
Eco130I CCWWGG 1 cut(s) 923
Eco24I GRGCYC 2 cut(s) 154, 340
Eco32I GATATC 1 cut(s) 388
Eco47I GGWCC 3 cut(s) 532, 538, 787
Eco53kI GAGCTC 1 cut(s) 152
Eco91I GGTNACC 1 cut(s) 1832
EcoICRI GAGCTC 1 cut(s) 152
EcoO109I RGGNCCY 4 cut(s) 224, 532, 787, 908
EcoO65I GGTNACC 1 cut(s) 1832
EcoRII CCWGG 3 cut(s) 403, 534, 1703
EcoRV GATATC 1 cut(s) 388
EcoT14I CCWWGG 1 cut(s) 923
EcoT38I GRGCYC 2 cut(s) 154, 340
ErhI CCWWGG 1 cut(s) 923
Esp3I CGTCTC 1 cut(s) 1817
FaeI CATG 8 cut(s) 236, 253, 298, 344, 641, 724, 880, 1034
FaqI GGGAC 2 cut(s) 545, 969
FatI CATG 8 cut(s) 232, 249, 294, 340, 637, 720, 876, 1030
FbaI TGATCA 1 cut(s) 352
Fnu4HI GCNGC 3 cut(s) 281, 1245, 1638
FokI GGATG 3 cut(s) 362, 602, 1050
FriOI GRGCYC 2 cut(s) 154, 340
Fsp4HI GCNGC 3 cut(s) 281, 1245, 1638
FspBI CTAG 3 cut(s) 984, 1662, 1773
GlaI GCGC 1 cut(s) 1285
GluI GCNGC 3 cut(s) 281, 1245, 1638
GsaI CCCAGC 1 cut(s) 1158
GsuI CTGGAG 1 cut(s) 505
HaeIII GGCC 3 cut(s) 226, 910, 1751
HapII CCGG 3 cut(s) 132, 275, 1811
HgaI GACGC 1 cut(s) 434
HhaI GCGC 1 cut(s) 1286
Hin1II CATG 8 cut(s) 236, 253, 298, 344, 641, 724, 880, 1034
Hin6I GCGC 1 cut(s) 1284
HinP1I GCGC 1 cut(s) 1284
HinfI GANTC 6 cut(s) 14, 396, 599, 919, 1094, 1256
HpaII CCGG 3 cut(s) 132, 275, 1811
HphI GGTGA 6 cut(s) 154, 199, 395, 814, 892, 1844
Hpy166II GTNNAC 2 cut(s) 310, 883
Hpy188I TCNGA 8 cut(s) 156, 174, 292, 450, 542, 586, 1003, 1077
Hpy188III TCNNGA 3 cut(s) 688, 799, 1031
Hpy8I GTNNAC 2 cut(s) 310, 883
HpyCH4III ACNGT 6 cut(s) 27, 812, 826, 1274, 1730, 1850
HpyCH4IV ACGT 2 cut(s) 492, 1840
HpyCH4V TGCA 5 cut(s) 467, 476, 641, 1008, 1247
HpyF10VI GCNNNNNNNGC 2 cut(s) 473, 989
HpyF3I CTNAG 6 cut(s) 153, 455, 585, 933, 1074, 1347
HpySE526I ACGT 2 cut(s) 492, 1840
Hsp92II CATG 8 cut(s) 236, 253, 298, 344, 641, 724, 880, 1034
HspAI GCGC 1 cut(s) 1284
Ksp22I TGATCA 1 cut(s) 352
Kzo9I GATC 7 cut(s) 352, 407, 594, 857, 936, 998, 1231
LmnI GCTCC 7 cut(s) 512, 704, 816, 1381, 1474, 1648, 1816
Lsp1109I GCAGC 3 cut(s) 267, 1231, 1649
LweI GCATC 1 cut(s) 118
MabI ACCWGGT 1 cut(s) 534
MaeI CTAG 3 cut(s) 984, 1662, 1773
MaeII ACGT 2 cut(s) 492, 1840
MaeIII GTNAC 5 cut(s) 205, 806, 948, 1213, 1832
MalI GATC 7 cut(s) 354, 409, 596, 859, 938, 1000, 1233
MboI GATC 7 cut(s) 352, 407, 594, 857, 936, 998, 1231
MflI RGATCY 1 cut(s) 407
MhlI GDGCHC 2 cut(s) 154, 340
MluCI AATT 8 cut(s) 331, 459, 610, 802, 1012, 1135, 1145, 1317
MlyI GAGTC 3 cut(s) 390, 608, 1088
MmeI TCCRAC 2 cut(s) 794, 1290
MseI TTAA 4 cut(s) 374, 462, 728, 1852
MslI CAYNNNNRTG 1 cut(s) 248
MspI CCGG 3 cut(s) 132, 275, 1811
MspR9I CCNGG 4 cut(s) 133, 405, 536, 1705
Mva1269I GAATGC 1 cut(s) 1010
MvaI CCWGG 3 cut(s) 405, 536, 1705
MwoI GCNNNNNNNGC 2 cut(s) 473, 989
NciI CCSGG 1 cut(s) 133
NdeII GATC 7 cut(s) 352, 407, 594, 857, 936, 998, 1231
NheI GCTAGC 1 cut(s) 983
NlaIII CATG 8 cut(s) 236, 253, 298, 344, 641, 724, 880, 1034
NlaIV GGNNCC 7 cut(s) 255, 369, 533, 706, 789, 818, 909
NmuCI GTSAC 2 cut(s) 205, 1832
PagI TCATGA 1 cut(s) 1030
PctI GAATGC 1 cut(s) 1010
PfeI GAWTC 3 cut(s) 14, 919, 1256
PflFI GACNNNGTC 1 cut(s) 536
PflMI CCANNNNNTGG 1 cut(s) 1069
PkrI GCNGC 3 cut(s) 282, 1246, 1639
PleI GAGTC 3 cut(s) 390, 607, 1088
PpsI GAGTC 3 cut(s) 390, 607, 1088
PpuMI RGGWCCY 2 cut(s) 532, 787
PshBI ATTAAT 1 cut(s) 462
Psp124BI GAGCTC 1 cut(s) 154
Psp1406I AACGTT 1 cut(s) 1840
Psp5II RGGWCCY 2 cut(s) 532, 787
Psp6I CCWGG 3 cut(s) 403, 534, 1703
PspEI GGTNACC 1 cut(s) 1832
PspFI CCCAGC 1 cut(s) 1154
PspGI CCWGG 3 cut(s) 403, 534, 1703
PspN4I GGNNCC 7 cut(s) 255, 369, 533, 706, 789, 818, 909
PspPI GGNCC 6 cut(s) 224, 532, 538, 787, 908, 1750
PspPPI RGGWCCY 2 cut(s) 532, 787
PstNI CAGNNNCTG 1 cut(s) 410
PsuI RGATCY 1 cut(s) 407
PsyI GACNNNGTC 1 cut(s) 536
RsaI GTAC 5 cut(s) 182, 423, 1053, 1276, 1727
RsaNI GTAC 5 cut(s) 181, 422, 1052, 1275, 1726
RseI CAYNNNNRTG 1 cut(s) 248
SacI GAGCTC 1 cut(s) 154
SaqAI TTAA 4 cut(s) 374, 462, 728, 1852
SatI GCNGC 3 cut(s) 281, 1245, 1638
Sau3AI GATC 7 cut(s) 352, 407, 594, 857, 936, 998, 1231
Sau96I GGNCC 6 cut(s) 224, 532, 538, 787, 908, 1750
SchI GAGTC 3 cut(s) 390, 608, 1088
ScrFI CCNGG 4 cut(s) 133, 405, 536, 1705
SduI GDGCHC 2 cut(s) 154, 340
SexAI ACCWGGT 1 cut(s) 534
SfaNI GCATC 1 cut(s) 118
SinI GGWCC 3 cut(s) 532, 538, 787
SmiMI CAYNNNNRTG 1 cut(s) 248
Sse9I AATT 8 cut(s) 331, 459, 610, 802, 1012, 1135, 1145, 1317
SsiI CCGC 1 cut(s) 400
SspI AATATT 1 cut(s) 775
SspMI CTAG 3 cut(s) 984, 1662, 1773
SstI GAGCTC 1 cut(s) 154
StyD4I CCNGG 4 cut(s) 131, 403, 534, 1703
StyI CCWWGG 1 cut(s) 923
TaaI ACNGT 6 cut(s) 27, 812, 826, 1274, 1730, 1850
TaiI ACGT 2 cut(s) 495, 1843
TaqI TCGA 5 cut(s) 549, 597, 602, 1230, 1269
TaqII GACCGA 2 cut(s) 455, 555
TasI AATT 8 cut(s) 331, 459, 610, 802, 1012, 1135, 1145, 1317
TatI WGTACW 3 cut(s) 421, 1274, 1725
TfiI GAWTC 3 cut(s) 14, 919, 1256
Tru1I TTAA 4 cut(s) 374, 462, 728, 1852
Tru9I TTAA 4 cut(s) 374, 462, 728, 1852
TscAI CASTG 3 cut(s) 529, 648, 1206
TseFI GTSAC 2 cut(s) 205, 1832
TseI GCWGC 3 cut(s) 280, 1244, 1637
Tsp45I GTSAC 2 cut(s) 205, 1832
TspDTI ATGAA 6 cut(s) 34, 232, 837, 865, 1019, 1047
TspGWI ACGGA 3 cut(s) 198, 232, 1841
TspRI CASTG 3 cut(s) 529, 648, 1206
Tth111I GACNNNGTC 1 cut(s) 536
Van91I CCANNNNNTGG 1 cut(s) 1069
VpaK11BI GGWCC 3 cut(s) 532, 538, 787
VspI ATTAAT 1 cut(s) 462
XapI RAATTY 2 cut(s) 610, 1012
XcmI CCANNNNNNNNNTGG 1 cut(s) 317
XspI CTAG 3 cut(s) 984, 1662, 1773
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.